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Developments of novel optical and electrical biosensors by NEMS and its applications
Journal of Bioscience and Bioengineering VOL. 108 No. S1, S147 – S164, 2009 www.elsevier.com/locate/jbiosc
Abstracts
Section X Micro/Nano Technology for Analysis and Cell Manipulation
MN-K1
We present a simple cell docking method induced by receding meniscus to capture non-adherent yeast cells onto microwells inside a microfluidic channel. Microwells were fabricated either by capillary moulding of UV curable polyurethane acrylate (PUA) onto glass substrate or direct replica moulding of poly(dimethyl siloxane) (PDMS). A cell suspension of the budding yeast, Saccharomyces cerevisiae, was introduced into the microfluidic channel by surface tension driven capillary flow and a receding meniscus was subsequently generated by evaporation. As the meniscus progressed, one to multiple yeast cells were spontaneously captured onto microwells by lateral capillary force created at the bottom of the meniscus. Using this cell-based platform, we observed the response of yeast cells upon stimulation by a mating pheromone (a-factor) by monitoring the expression of green fluorescent protein (GFP) with time.
Au-coated surface of Surface plasmon resonance was modified by co-sputtering of nanoparticle. These nanoparticles enhanced the sensitivity of SPR signal that reached 10−15 fg/mm2 and this was documented by ssDNA hybridization. Also, integration of commonpath phase-shift interferometry with SPR makes SPR with high throughput capability was launched and reported by our group. Colorimetric resonant optical biosensor (guided mode resonance, GMR) was originally designed as an optical component for filtration of certain wavelengths and was based on the surface of a narrow bandwidth guided mode resonant. We successfully designed the sensor structure and fabricated by NEMS facilities and with the immobilized thermobin DNA aptamer, the binding behaviors of thrombin with the aptamer were studied. Interdigitated microelectrodes (IME) were used as impedance sensors for rapid detection of protein. The impedance variation was due to protein attached on electrode surface, which affect the ionic composition of the double layer. The interdigitated microelectrodes were fabricated by photolithography and applied to detect protein quantitatively. Finally, we used metal-assisted etching process to fabricate a nanofilament structure on silicon substrate for matrix-free MS technology and setting optimal surface structure by MS intensity with different porosity and pore depth. The detection sensitivity of the n-filament Si MS reached 15 amol of oligopeptide.
doi:10.1016/j.jbiosc.2009.08.394
doi:10.1016/j.jbiosc.2009.08.395
MN-K2
MN-K3
Developments of novel optical and electrical biosensors by NEMS and its applications
Nanobioelectronic nose using nanotubes combined with human olfactory receptors
Wen-Yih Chen,1 Jang-Zern Tsai,2 Jenq-Yang Chang,3 Tsung-Hsun Yang,3 and Chia-Wen Tsao4
Tai Hyun Park
Receding meniscus induced docking of yeast cells for quantitative single-cell analysis Min-Cheol Park, Jae-Yong Hur, Sang-Hyun Park, and Kahp-Yang Suh Seoul National University, Seoul, Republic of Korea
Seoul National University, Seoul, Republic of Korea Department of Chemical and Materials Engineering, National Central University, Jhong-Li, Taiwan 1 Department of Electrical Engineering, National Central University, Jhong-Li, Taiwan 2 Department of Optics and Photonics, Jhong-Li, Taiwan 3 and Department of Mechanical Engineering, National Central University, Jhong-Li, Taiwan 4 This paper presents recent developments of using NEMS technologies on non-labeling biosensors such as surface plasmon resonance (SPR), guided mode resonance (GMR), interdigitated microelectrode (IME) impedance. Also, metal-assisted etching process nanofilament based surface-assisted/enhanced laser desorption/ionization mass spectroscopy is discussed.
The olfactory system plays an important role in recognizing environmental conditions. Since olfactory receptor genes were identified and cloned, various researches on olfactory systems have been carried out, and the interest in olfaction research has been increasing due to its potential industrial applications. Odor discrimination is a challenging research subject for key applications in fields of food/beverage, environmental monitoring, and disease diagnosis. In the smelling process, the binding of specific odorants to the olfactory receptor proteins is the initiation step in odor recognition and triggers signal transduction in a cell. Functional expression of the olfactory receptors on the surface of culturable cells is very useful for
Abstracts
Section X Micro/Nano Technology for Analysis and Cell Manipulation
MN-K1
We present a simple cell docking method induced by receding meniscus to capture non-adherent yeast cells onto microwells inside a microfluidic channel. Microwells were fabricated either by capillary moulding of UV curable polyurethane acrylate (PUA) onto glass substrate or direct replica moulding of poly(dimethyl siloxane) (PDMS). A cell suspension of the budding yeast, Saccharomyces cerevisiae, was introduced into the microfluidic channel by surface tension driven capillary flow and a receding meniscus was subsequently generated by evaporation. As the meniscus progressed, one to multiple yeast cells were spontaneously captured onto microwells by lateral capillary force created at the bottom of the meniscus. Using this cell-based platform, we observed the response of yeast cells upon stimulation by a mating pheromone (a-factor) by monitoring the expression of green fluorescent protein (GFP) with time.
Au-coated surface of Surface plasmon resonance was modified by co-sputtering of nanoparticle. These nanoparticles enhanced the sensitivity of SPR signal that reached 10−15 fg/mm2 and this was documented by ssDNA hybridization. Also, integration of commonpath phase-shift interferometry with SPR makes SPR with high throughput capability was launched and reported by our group. Colorimetric resonant optical biosensor (guided mode resonance, GMR) was originally designed as an optical component for filtration of certain wavelengths and was based on the surface of a narrow bandwidth guided mode resonant. We successfully designed the sensor structure and fabricated by NEMS facilities and with the immobilized thermobin DNA aptamer, the binding behaviors of thrombin with the aptamer were studied. Interdigitated microelectrodes (IME) were used as impedance sensors for rapid detection of protein. The impedance variation was due to protein attached on electrode surface, which affect the ionic composition of the double layer. The interdigitated microelectrodes were fabricated by photolithography and applied to detect protein quantitatively. Finally, we used metal-assisted etching process to fabricate a nanofilament structure on silicon substrate for matrix-free MS technology and setting optimal surface structure by MS intensity with different porosity and pore depth. The detection sensitivity of the n-filament Si MS reached 15 amol of oligopeptide.
doi:10.1016/j.jbiosc.2009.08.394
doi:10.1016/j.jbiosc.2009.08.395
MN-K2
MN-K3
Developments of novel optical and electrical biosensors by NEMS and its applications
Nanobioelectronic nose using nanotubes combined with human olfactory receptors
Wen-Yih Chen,1 Jang-Zern Tsai,2 Jenq-Yang Chang,3 Tsung-Hsun Yang,3 and Chia-Wen Tsao4
Tai Hyun Park
Receding meniscus induced docking of yeast cells for quantitative single-cell analysis Min-Cheol Park, Jae-Yong Hur, Sang-Hyun Park, and Kahp-Yang Suh Seoul National University, Seoul, Republic of Korea
Seoul National University, Seoul, Republic of Korea Department of Chemical and Materials Engineering, National Central University, Jhong-Li, Taiwan 1 Department of Electrical Engineering, National Central University, Jhong-Li, Taiwan 2 Department of Optics and Photonics, Jhong-Li, Taiwan 3 and Department of Mechanical Engineering, National Central University, Jhong-Li, Taiwan 4 This paper presents recent developments of using NEMS technologies on non-labeling biosensors such as surface plasmon resonance (SPR), guided mode resonance (GMR), interdigitated microelectrode (IME) impedance. Also, metal-assisted etching process nanofilament based surface-assisted/enhanced laser desorption/ionization mass spectroscopy is discussed.
The olfactory system plays an important role in recognizing environmental conditions. Since olfactory receptor genes were identified and cloned, various researches on olfactory systems have been carried out, and the interest in olfaction research has been increasing due to its potential industrial applications. Odor discrimination is a challenging research subject for key applications in fields of food/beverage, environmental monitoring, and disease diagnosis. In the smelling process, the binding of specific odorants to the olfactory receptor proteins is the initiation step in odor recognition and triggers signal transduction in a cell. Functional expression of the olfactory receptors on the surface of culturable cells is very useful for