Diagnosis of Leishmania species from formalin-fixed biopsy samples of ecuadorian patients by PCR

Poster SeSSionSI hzrasito~ogy InternaIiond

47

(Suppl.)

(1998)

283-389

369

G. Diagnosis P-0926

LEISHMANIASIS ASPIRATES WITH

DIAGNOSIS

BY PCR ON LYMPH

AND PERIPHERAL

INDIRECT

IMMUNO

BLOOD.

NODE

COMPARISON

FUORESCENCE

P-0928

SPECIES FROM FORMALIN-

DIAGNOSIS OF LElSHMANlA FIXED

METHOD.

BIOPSY SAMPLES OF ECUADORIAN

PATIENTS

BY PCR Mimori I’, Matsumoto

p, Sasaki J’, Nakala MC, Gomez Ed, Uezato Hr.

Nonaka S, Furuya M’, Saya H’ and Hashiguchi ‘Department

of Tumor Genetics

School of Medicine, Japan, %inkyo ‘Biomedical

R&D Department,

Yokohama,

Universidad

Ecuador,

‘Department

Comprehensive

Kumamoto

Electric

de Medicina

Industries,

Tropical,

of Dermatology

Japan, LTD,

Facultad

Catolica de Santiago de Guayaquil,

de

Guayaquil,

and the Research

Medicine, Faculty of Medicine, University

Japan,‘Instltule

University

Medical College, Kumamoto,

Sumitomo

Japan, dDepartmento

Medicina,

Yg

and Biology,

Center

of

of Ryukyus,

for Laboratory Animals and ~Deparbnent of Parasitology,

Kochi Medical School, Kochi, Japan The precise diagnosis suitsble treahnent

of Leishmamo species is unportant

for doing

against each Leishmaniaspecies since different specws

cause different clinical features of the disease.

We have developed

highly specific PCR panel to enable the idenhfication

a

of the hvo Leishma-

groups and the five major Leishmaniospecies which cause

ma subgenus

New World Cutaneous

Lelshmanmses

this panel were deslgned

(NWCL).

to distinguish

The primers

the difference

used

disposrtion

for

of

nucleotldes (polymorphism) in sequences of commonly amplified DNA bands of the parasites produced by arbitrarily-pruned (AI’)-PCR. Using these polymorphism

al species-level

specific primers, we could identify Leishmpnio

strains

by polymorplusm-specific (PS)-PCR. Diagnosis of PSPCR

was performed with formalin-fixed biopsy specimens of the leishmanial lesions from patrents in Ecuador, South America, and these lesions were detemuned to be caused by L CVtanniaJ mexicana.

Our

ponamensis,

and L.&ishmaniaJ

PCR panel may offer an important and practical approach

to the standardized identiflcatlon of Leishmnma

species in field examina-

tions.

P-0927

P-0929 NALUATION

SERODIAGNOSIS

OF

NORTHWESTERN

CHINA:

ANTIGEN VS.

(RK39l

IN

PROMASTIGOTE

RAPID

LEISHMRNIASIS

SCREENING

DIPSTICK

WITH

FORMAT

ANC

A

IN

RECOMBINANT

ITS

Shulidan K'*,

I*,

Zuo

XP*, Chal a*. Reed S*+* AND

MatsumotoI’+*,

KP'+'

*Institute

of

Parasltlc

Diseases.

Chinese

Academy

of

Preventive Medicine, Shanghai, Chlna, **Institute of Science, Animal ReSOUrCe Faculty of Agriculture, University

of

University

of

School,

Tokyo,

North We

the

format

The a

Patients

product

kinesin-like and a

gene

compared

antigens. with

rapld

serologically

recombinant

used

successful

for

Laishmaniasis(VLj diagnosed

Sciences/The

Rapid

drop

of

of

blood

for

positive

indicated

more

by

and of

specific

and

in

Medical

acid

in

of

blot

from

lrK39)

was as

the

at the scene using rK39 1"

dntl-rK39

antibodles

in

dipsticks,

the

analysis titration

than

China

lysates

achieved ':erum or

further

sensitive

rK39

repeats

chaqasi

reactlox

end-point

studies

of

northwestern

amino

presence

Western

their

these

39

was

accounts

results

VL

of

diagnosx whole

aypllcation

promastiyote

The

the

for

with

format.

collected

**+Frnch

serodiagnosis of Visceral with splenomegaly were

Lelshmania

dipstick as

and Chicago

Chicago,USA

report

dipstick

Japan

Tokyo,

Health

llldicate

of by

the

promastigote

sera

ELISA.

that

rK3Y

OF W

wmlw

The 1s

lysates.

P.MWN

y L (v-t

ANllQENS IN SKIN TEST Mmava G.. Terms Y.. F&&-r H. Mmd@&n, Nabonal Health InstiMe. Lh - FW

SPECIFICITY

ANTIGENS

Qu JQ’. Feng Q*, Kawazu s**, Katakura Chang

VISCERAL

L ., Va,j8

S.

bnzl,inuis