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Diastolic function in systemic lupus erythematosus with and without high levels of antiphospholipid antibodies
J Mol Cell Cardiol 24 (Supplement VI) (1992) 139
Diastolic function in systemic lupus erythematosus with and without high levels of antiphospholipid antibodies N. Coudrav. D.de Zuttere. 0. Bletrv, J.C. Pournv, J. P. Beregi, P. Godeau, B. Wechsler, Y. Lecarnentier and D. Chemla. Inserm .U27.5-Ldk-ENSTA-Ecole Polytechnique, Hop Bichat, Piti&SalpCt&e and Kremlin-Bicttre, Paris, France. To investigate the relation between diastolic dysfunction and the presence of high levels of antiphospholipid antibodies (APL) in patients with systemic lupus erythematosus (SLE), computerassisted M-mode echocardiography was performed in 41 SLE (34F/7M, 38+/-6 years) and in 15 normal subjects. Peak rate of left ventricular enlargement in diastole (D+) (11.98+/-0.47 vs 13.92+/-0.68 cm/s, u=O.O3). oeak rate of nosterior wall thinning (PWT) (7.44+/-0.41 vs 9.89+/-0.57 cm/s. ~=0.002) and maximal diastolic endocardial velocity of theposteribr~wall (PWV) (9.49+/-0.43 vs 11.53+/-0.56 cm/s, p=O.Ol) were lower in SLE than in controls, respectively. Conversely, fractionnal shortening, enddiastolic and end-systolic LV dimensions were not statistically different between SLE and control patients. In SLE, a circulating anticoagulant, or an anticardiolipin antibody IgG>lSui or IgM>6ui, or a falseVDRL were observed in 24 patients (APL+) but not in 17 patients (APL-). Although the 3 studied diastolic parameters were more impaired in APL+ than in APL-, there was no statistically significant difference between APL+ and APL- as regards D+ (p=O.35), PWT (p=O.34) and PWV (p=O.67). Our results indicate that there was no relation between the impairment of diastolic parameters and the presence of high levels of antiphospholipid antibodies in patients with systemic lupus erythematosus.
140
IMPAIRED EARLY VALVULOPLASTY
DIASTOLIC
FUNCTION
IN
MITRAL
STENOSIS
: INFLUENCE
OF
BALLOON
Michel S. SLAMA, Yves LECARPENTIER, Luc H. DRIEU, Jean-Paul BEREGI, Nicolas COUDRAY, Gilbert MOTTE, Denis CHEMLA. HBpital A. B&l&e, Clamart, INSERM U275, Palaiseau, France. It is know that the peak rate of LV dimension increase in early diastole CD+) is linked to both the extent of LV shortening (dD) and the peak rate of posterior wall thinning (E+). To determine the influence of balloon mitral valvuloplasty (BMW) on LV early diastolic function, digitized M-mode echocardiography was performed in 7 patients (32*4 yrs) with severe, pure, non calcified mitral stenosis (MS) both before (mitral valve area MVA = 1 .I +O.l cm*) and 48 hrs after successful BMV (MVA= 2.1 & 1 0.1 cm*), and in 10 controls (C, 27& 1 yrs, NS). In C, D+ was positively related to dD (r=0.89. o
141
SIMILARITY OF SR CAPACITY IN HUMAN AND FERRET HEARTS . -,C Huchet M.Ferrier, S.Baudet, J.Noireaud, C.LCoty. URA CNRS 1340, Laboratory of General Physiology, E.N.V., F44087 NANTES Cedex 03 France. The caffeine (2-20 mM) contracture developped by saponin-skinned (50 p&ml) cardiac fibers has been used to compare the sarcoplasmic reticulum (SR) content in papillary muscles isolated from the right ventricle of ferret and human hearts. The human heart was obtained fom an heart-lung transplanted patient aged 32 years with cystic fibrosis. Following a loading time (8 min), the application of 10 n&lcaffeine induced a transient contracture which time constants of relaxation were 77.2 k10.9 set in ferret heart and 75.7 +l.O set in human preparation. In both muscles the amplitude of the contractile response was related to the caffeine concentration by a rectangular hyperbola. The Km were 1.5 +0.5 mM and 1.67 &I.4 mM in ferret and human heart respectively. Addition of the calcium antagonist Bepridil(50 @I) in loading solutions resulted in a decrease of the amplitude of the transient caffeine (10 mM) contracture by 15.0 f 0.5% in ferret fibers and 13.1 M.8 % in human ones. This effect was directly related to a reduction in the calcium content of the SR. The present experiment has shown that the sensivities of the SR to caffeine and Bepridil were very similar in both right ventricles and suggested that some of results obtained in saponin-skinned fibers from ferret heart could be extended to the human cardiac muscle. This work
was supported
by the INSERM,
the FRM, s.51
and the Fondation
Langlois
Diastolic function in systemic lupus erythematosus with and without high levels of antiphospholipid antibodies N. Coudrav. D.de Zuttere. 0. Bletrv, J.C. Pournv, J. P. Beregi, P. Godeau, B. Wechsler, Y. Lecarnentier and D. Chemla. Inserm .U27.5-Ldk-ENSTA-Ecole Polytechnique, Hop Bichat, Piti&SalpCt&e and Kremlin-Bicttre, Paris, France. To investigate the relation between diastolic dysfunction and the presence of high levels of antiphospholipid antibodies (APL) in patients with systemic lupus erythematosus (SLE), computerassisted M-mode echocardiography was performed in 41 SLE (34F/7M, 38+/-6 years) and in 15 normal subjects. Peak rate of left ventricular enlargement in diastole (D+) (11.98+/-0.47 vs 13.92+/-0.68 cm/s, u=O.O3). oeak rate of nosterior wall thinning (PWT) (7.44+/-0.41 vs 9.89+/-0.57 cm/s. ~=0.002) and maximal diastolic endocardial velocity of theposteribr~wall (PWV) (9.49+/-0.43 vs 11.53+/-0.56 cm/s, p=O.Ol) were lower in SLE than in controls, respectively. Conversely, fractionnal shortening, enddiastolic and end-systolic LV dimensions were not statistically different between SLE and control patients. In SLE, a circulating anticoagulant, or an anticardiolipin antibody IgG>lSui or IgM>6ui, or a falseVDRL were observed in 24 patients (APL+) but not in 17 patients (APL-). Although the 3 studied diastolic parameters were more impaired in APL+ than in APL-, there was no statistically significant difference between APL+ and APL- as regards D+ (p=O.35), PWT (p=O.34) and PWV (p=O.67). Our results indicate that there was no relation between the impairment of diastolic parameters and the presence of high levels of antiphospholipid antibodies in patients with systemic lupus erythematosus.
140
IMPAIRED EARLY VALVULOPLASTY
DIASTOLIC
FUNCTION
IN
MITRAL
STENOSIS
: INFLUENCE
OF
BALLOON
Michel S. SLAMA, Yves LECARPENTIER, Luc H. DRIEU, Jean-Paul BEREGI, Nicolas COUDRAY, Gilbert MOTTE, Denis CHEMLA. HBpital A. B&l&e, Clamart, INSERM U275, Palaiseau, France. It is know that the peak rate of LV dimension increase in early diastole CD+) is linked to both the extent of LV shortening (dD) and the peak rate of posterior wall thinning (E+). To determine the influence of balloon mitral valvuloplasty (BMW) on LV early diastolic function, digitized M-mode echocardiography was performed in 7 patients (32*4 yrs) with severe, pure, non calcified mitral stenosis (MS) both before (mitral valve area MVA = 1 .I +O.l cm*) and 48 hrs after successful BMV (MVA= 2.1 & 1 0.1 cm*), and in 10 controls (C, 27& 1 yrs, NS). In C, D+ was positively related to dD (r=0.89. o
141
SIMILARITY OF SR CAPACITY IN HUMAN AND FERRET HEARTS . -,C Huchet M.Ferrier, S.Baudet, J.Noireaud, C.LCoty. URA CNRS 1340, Laboratory of General Physiology, E.N.V., F44087 NANTES Cedex 03 France. The caffeine (2-20 mM) contracture developped by saponin-skinned (50 p&ml) cardiac fibers has been used to compare the sarcoplasmic reticulum (SR) content in papillary muscles isolated from the right ventricle of ferret and human hearts. The human heart was obtained fom an heart-lung transplanted patient aged 32 years with cystic fibrosis. Following a loading time (8 min), the application of 10 n&lcaffeine induced a transient contracture which time constants of relaxation were 77.2 k10.9 set in ferret heart and 75.7 +l.O set in human preparation. In both muscles the amplitude of the contractile response was related to the caffeine concentration by a rectangular hyperbola. The Km were 1.5 +0.5 mM and 1.67 &I.4 mM in ferret and human heart respectively. Addition of the calcium antagonist Bepridil(50 @I) in loading solutions resulted in a decrease of the amplitude of the transient caffeine (10 mM) contracture by 15.0 f 0.5% in ferret fibers and 13.1 M.8 % in human ones. This effect was directly related to a reduction in the calcium content of the SR. The present experiment has shown that the sensivities of the SR to caffeine and Bepridil were very similar in both right ventricles and suggested that some of results obtained in saponin-skinned fibers from ferret heart could be extended to the human cardiac muscle. This work
was supported
by the INSERM,
the FRM, s.51
and the Fondation
Langlois