- Email: [email protected]
Dietary Sulforaphane supplementation induces Nrf2, attenuating hypoxia-induced vascular smooth muscle cell proliferation and remodelling following carotid artery ligation
D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107
Acknowledgements
Supported by KCL Summer Studentship. http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.205
P-121
Metabolic activity of radish sprouts isothiocyanates in Drosophila melanogaster
derived
Nieves Baenas 1,3, Stefanie Staats 2, Anke Schloesser 2, Diego A Moreno 3, Cristina García-Viguera 3, Gerald Rimbach 2, Anika E Wagner 1,2 1
Institute of Nutritional Medicine, University of Luebeck, Luebeck, Germany 2 Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany 3 National Research Council, Murcia, Spain
We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/l) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster take up and metabolize isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of αamylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus. E-mail address: [email protected] (A. Wagner) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.206
P-122
The effects of taurine on the levels of GSH and LPO on in vitro glucose-induced cataractous rabbit lenses Ali Mert Ozgonul 2, Sibel Konyalioglu 1 1
Ege University, Faculty of Medicine, Department of Biochemistry, Izmir, Turkey 2 Ege University, Faculty of Pharmacy, Department of Biochemistry, Izmir, Turke Keywords: Taurine; cataract; lipidperoxidation; reduced glutathione
lenses. With this aim lens samples were divided into two parts as cortex and nucleus, then the GSH and LPO levels were determined under the headings of control, cataract and taurine study groups. According to our results, in the nucleus samples the GSH levels in the cataract and taurine groups showed no remarkable differences as compared to the controls (respectively p ¼0.413 and p¼ 0.792). however, in the cortex samples the GSH levels in the cataract group decreased significantly relative to the controls (p ¼0.033). There was no considerable difference between the GSH levels in the taurine and the control groups of the cortex samples (p ¼0.295). Concerning the LPO levels, the control, cataract and taurine groups of the nucleus samples displayed no significant differences, whereas in the cortex samples, the cataract group was found to have significantly increased LPO levels as compared to the controls (p ¼0.022). LPO levels of the taurine group tended to yield similar results to those of the control group. According to GSH and LPO results in the taurine groups, it can be postulated that taurine may protect lens membrane lipids against oxidative destruction. E-mail address: [email protected] (A.M. Ozgonul) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.207
P-123
Circadian clock as possible protective mechanism to pollution induced skin damage Mascia Benedusi 1, Elena Frigato 1, Mattia Beltramello 1, Cristiano Bertolucci 1, Giuseppe Valacchi 1,2 1 2
University of Ferrara, Italy NC State University, Kannapolis, NC, USA
Ozone (O3) is among the most toxic stressors to which living organisms are continuously exposed and the skin is one of the most susceptible tissues to environmental damage. Many data suggests a significant role of the circadian system in the regulation of protein involved in the cellular response to oxidative stress. However, there is still an incomplete understanding of the molecular mechanism linking circadian rhythms and cellular defensive pathway. Here we investigated a possible protective role of the circadian system to O3 induced damage in human keratinocytes. Our results showed that, mimicking environmental condition of O3 exposure, synchronized keratinocytes exhibited a more efficient antioxidant response, attested by a faster activation of NRF2, compared to the arrhythmic ones.Analysis of clock gene mRNA level in rhythmic cells reveals a more rapid induction of Bmal1. Tthese data suggested that in keratinocytes,Bmal1 regulates the expression of Nrf2. Based on this findings, we suppose that an adequate coordination of the circadian system and antioxidant pathway might be essential to maintain the homeostasis in the skin. Alteration of metabolic pathways as occurs in many diseases or irregular schedule of life activity (shift work, transcontinental journey) could negatively influencegene expression and associated organ physiology via its effect on the circadian system. E-mail address: [email protected] (M. Benedusi) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.208
In this our study, we try to determine whether taurine has anticataractogenic action by the levels of glutathione (GSH) and lipid peroxidation (LPO) in vitro glucose-catarct induced rabbit
S59
Acknowledgements
Supported by KCL Summer Studentship. http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.205
P-121
Metabolic activity of radish sprouts isothiocyanates in Drosophila melanogaster
derived
Nieves Baenas 1,3, Stefanie Staats 2, Anke Schloesser 2, Diego A Moreno 3, Cristina García-Viguera 3, Gerald Rimbach 2, Anika E Wagner 1,2 1
Institute of Nutritional Medicine, University of Luebeck, Luebeck, Germany 2 Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany 3 National Research Council, Murcia, Spain
We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/l) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster take up and metabolize isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of αamylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus. E-mail address: [email protected] (A. Wagner) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.206
P-122
The effects of taurine on the levels of GSH and LPO on in vitro glucose-induced cataractous rabbit lenses Ali Mert Ozgonul 2, Sibel Konyalioglu 1 1
Ege University, Faculty of Medicine, Department of Biochemistry, Izmir, Turkey 2 Ege University, Faculty of Pharmacy, Department of Biochemistry, Izmir, Turke Keywords: Taurine; cataract; lipidperoxidation; reduced glutathione
lenses. With this aim lens samples were divided into two parts as cortex and nucleus, then the GSH and LPO levels were determined under the headings of control, cataract and taurine study groups. According to our results, in the nucleus samples the GSH levels in the cataract and taurine groups showed no remarkable differences as compared to the controls (respectively p ¼0.413 and p¼ 0.792). however, in the cortex samples the GSH levels in the cataract group decreased significantly relative to the controls (p ¼0.033). There was no considerable difference between the GSH levels in the taurine and the control groups of the cortex samples (p ¼0.295). Concerning the LPO levels, the control, cataract and taurine groups of the nucleus samples displayed no significant differences, whereas in the cortex samples, the cataract group was found to have significantly increased LPO levels as compared to the controls (p ¼0.022). LPO levels of the taurine group tended to yield similar results to those of the control group. According to GSH and LPO results in the taurine groups, it can be postulated that taurine may protect lens membrane lipids against oxidative destruction. E-mail address: [email protected] (A.M. Ozgonul) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.207
P-123
Circadian clock as possible protective mechanism to pollution induced skin damage Mascia Benedusi 1, Elena Frigato 1, Mattia Beltramello 1, Cristiano Bertolucci 1, Giuseppe Valacchi 1,2 1 2
University of Ferrara, Italy NC State University, Kannapolis, NC, USA
Ozone (O3) is among the most toxic stressors to which living organisms are continuously exposed and the skin is one of the most susceptible tissues to environmental damage. Many data suggests a significant role of the circadian system in the regulation of protein involved in the cellular response to oxidative stress. However, there is still an incomplete understanding of the molecular mechanism linking circadian rhythms and cellular defensive pathway. Here we investigated a possible protective role of the circadian system to O3 induced damage in human keratinocytes. Our results showed that, mimicking environmental condition of O3 exposure, synchronized keratinocytes exhibited a more efficient antioxidant response, attested by a faster activation of NRF2, compared to the arrhythmic ones.Analysis of clock gene mRNA level in rhythmic cells reveals a more rapid induction of Bmal1. Tthese data suggested that in keratinocytes,Bmal1 regulates the expression of Nrf2. Based on this findings, we suppose that an adequate coordination of the circadian system and antioxidant pathway might be essential to maintain the homeostasis in the skin. Alteration of metabolic pathways as occurs in many diseases or irregular schedule of life activity (shift work, transcontinental journey) could negatively influencegene expression and associated organ physiology via its effect on the circadian system. E-mail address: [email protected] (M. Benedusi) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.208
In this our study, we try to determine whether taurine has anticataractogenic action by the levels of glutathione (GSH) and lipid peroxidation (LPO) in vitro glucose-catarct induced rabbit
S59