- Email: [email protected]
Differential diagnosis between Taenia asiatica and T. Saginata adult tapeworms by enzyme-linked immunoelectrotransfer blot (EITB)
Oral Sessions
O-0398
/ Parasitology lnternarionaI47 (Suppl.) (1998) 133-281
ASSESSMENT OF OPTIMAL, AN IMMUNOCHROMATOGRAPHIC TEST FOR MALARIA, IN AREFERENCE CENTRE.
0-0~
DIFFERENTIAL DIACWOSIS BETMEN T4EN/A AS/A77CA AND T. SACYNATA ADUT ThEWORMS BY ENZYMELINKED IMWN(XLECTROTRANSFER MOT (EITB)
lea&i,
C ‘odii PL, Moody AH, Hunt Cooke AH D&rtment of Clinical Parasitology, The Hospital for Tropical Diseases, London NW1 OPE, UK
Eom K
Department
of
University,
Parasitology,
Chongju
City,
Enzyme-linked the
Blood iilm diagnosis of malaria requires a laboratory and significant technical expertise. OptiMAL is a non-microscopic strip test, which uses immunochromatographic technology to detect parasite laaate dehydrogenase specific for Pkwnoahm Iorcippvnr and P. viw. The performance of Optih4AL compared to microscopy for the diagnosis of malaria in a reference centre wiU be described.
reactivities
diagnoagnosis
of
taeniases
asiatica adult
proglottids
of
gel.
were
reactive and
or over
of
observed
between
49. 46, 40. 37 and fifteen
visible
96. 85. different
bands
individuals.
The Antigen
and
49, 45,
41,
taeniasis
19. 13kDa
kDa
in
well
major of
absorbaxes
91.
thus
they
is possible
aisatica.
cestodiases
10%
rextivities
adult
and
larval
antigens.
bands
of
80.
showed
at
113.
107.
(120.
73, least 102.
were
common revealed
in
those
distinct
69.
against
the
taeniasis
81.
73,
64,
52,
lacked
the
113, 107. 85. 69. 45,
lacked
asiatica
37kDa
bands
the 107. 85, 69. 58, 49, 45,
be differentiated
from
taeniases.
tapeworm antigens showed distinct in taeniasis saginata while showing
These
between
19kDa
tapeworm
85,
could
ElTl3
with
13kDa) by ElTB against five collected from five different
bands
and the sparganosis
bands:
major
bands
from
the
tapeworm
serum
and
Cysticercosis
cross
in all
Soluble
by
system
with
T. asMica
immunoblot
gravid
SDS-PAGE.
control
25, 19. antigens
immunoblot
with
of
nana infections were easily Thirty-six component proteins
13 kDa
asiatica 12CkDa
adult
different
taeniasis
as other
sera
5ug/ml)
observed
revealed
and T
On the other hand, T. saginata and 40 kDa immunoblot bands diagnosis
were
by
extracted
proteins
infected
and H. infections.
between
saginata.
bands
41. 31. 25kDa
IgG
for Cross
evaluated saline
concentration,
buffer
45, 41. 31, adult worm
3lkDa
comparatwely
against
121
14 kDa.
69. 58, 49, T. asiatica
specimens. 58.
the
discontinuous ELISA
attempted human.
were
using
T. asiatica or T. saginata adult
against
The sparganum. D. /aturn differentiated from Taenia were
in
by reducing
serum
tapeworm
was
Component
observed to
Wl3~
cestodiases
IgC.
National
of Korea
asiatica
(ELlSA)
(protein
Tricine
raenia
0.210
infections
other
Chungbuk
Republic
blot
assay
antigen
T. asiatica
SDS-PACE
by showing
Medicine,
taeniasis
anti-human
proteins
separating
and
worm
conjugated
using
of
360-763.
of
immunosorbent
peroxidase component
College
Chungbuk
immuncelectrotransfer
differential
enzyme-linked T
225
results
T. asia&
show and
r
that sagin&
the
69 58
differential
infections
by EITB
o-0401 AND PENTASTOMIOSIS IN CHINA PATHOLOGICAL DIAGNOSIS AND II. OF DIFFERENTIAL DIAGNOSIS PENTASTOMIOSIS
PENTASTOMIDS
Rang Yun Lung’ leu Ming Hwa** Ma Kuo Chun’ *Dept. Pathology, Shanghai Medical University. Shanghai, Chrna **Dept. Parasitology, Zhejiang Medical University. Hangzhou, China Nme pathologically studied cases of this rare parasitic drsease collected during 1950’s to 1980’s in Shanghai and Chungqing arc reported. In China we have to emphasize Ihe distmction between the etiopathological diagnosis and presumpuve pathologIca diagnosis of this disease and the sigmficance of its diffcrcntlal diagnons. The reason is that these parasitic pathogens used to be faded away entirely from the lesions in the majority of these cases (6 out of 9). In only 3 cases the precious pathognamonic relics _- the calcified cuticlechtmous oral parts of the pentastomids - had been found in those typical granulomatous lesions, however, the ldenbtication of those characteristic oral parts could only be traced to tbe Armrllr/er sp. Therefore. m most cases, the presumptive pathological diagnoses of ths disease were necessary. They wele made by a series of ‘specific’ pathological characteristics, such as the protuberant ‘pearls’ (sclerotic nodules), the concentric laminations of calcification (targetoid appearance) in a focus of caseous necrosw and the relalively specitic distributional pattern In the differential diagnosis the possibilities of tuberculosis, Pick’s drsease; and the diseases caused by other parasites, such as C~SI~CWCUS cellulose, S’arganum, Echrnococcus granubsus and Diplera larva should be ruled out. To our knowledge the case of pcntaslomiotrc myocarditis might be considered as the first report in the literature The lymphohematogenaus spread of their primary larvae might be responsible for this rntracardlac mvolvemenl.
as
O-0398
/ Parasitology lnternarionaI47 (Suppl.) (1998) 133-281
ASSESSMENT OF OPTIMAL, AN IMMUNOCHROMATOGRAPHIC TEST FOR MALARIA, IN AREFERENCE CENTRE.
0-0~
DIFFERENTIAL DIACWOSIS BETMEN T4EN/A AS/A77CA AND T. SACYNATA ADUT ThEWORMS BY ENZYMELINKED IMWN(XLECTROTRANSFER MOT (EITB)
lea&i,
C ‘odii PL, Moody AH, Hunt Cooke AH D&rtment of Clinical Parasitology, The Hospital for Tropical Diseases, London NW1 OPE, UK
Eom K
Department
of
University,
Parasitology,
Chongju
City,
Enzyme-linked the
Blood iilm diagnosis of malaria requires a laboratory and significant technical expertise. OptiMAL is a non-microscopic strip test, which uses immunochromatographic technology to detect parasite laaate dehydrogenase specific for Pkwnoahm Iorcippvnr and P. viw. The performance of Optih4AL compared to microscopy for the diagnosis of malaria in a reference centre wiU be described.
reactivities
diagnoagnosis
of
taeniases
asiatica adult
proglottids
of
gel.
were
reactive and
or over
of
observed
between
49. 46, 40. 37 and fifteen
visible
96. 85. different
bands
individuals.
The Antigen
and
49, 45,
41,
taeniasis
19. 13kDa
kDa
in
well
major of
absorbaxes
91.
thus
they
is possible
aisatica.
cestodiases
10%
rextivities
adult
and
larval
antigens.
bands
of
80.
showed
at
113.
107.
(120.
73, least 102.
were
common revealed
in
those
distinct
69.
against
the
taeniasis
81.
73,
64,
52,
lacked
the
113, 107. 85. 69. 45,
lacked
asiatica
37kDa
bands
the 107. 85, 69. 58, 49, 45,
be differentiated
from
taeniases.
tapeworm antigens showed distinct in taeniasis saginata while showing
These
between
19kDa
tapeworm
85,
could
ElTl3
with
13kDa) by ElTB against five collected from five different
bands
and the sparganosis
bands:
major
bands
from
the
tapeworm
serum
and
Cysticercosis
cross
in all
Soluble
by
system
with
T. asMica
immunoblot
gravid
SDS-PAGE.
control
25, 19. antigens
immunoblot
with
of
nana infections were easily Thirty-six component proteins
13 kDa
asiatica 12CkDa
adult
different
taeniasis
as other
sera
5ug/ml)
observed
revealed
and T
On the other hand, T. saginata and 40 kDa immunoblot bands diagnosis
were
by
extracted
proteins
infected
and H. infections.
between
saginata.
bands
41. 31. 25kDa
IgG
for Cross
evaluated saline
concentration,
buffer
45, 41. 31, adult worm
3lkDa
comparatwely
against
121
14 kDa.
69. 58, 49, T. asiatica
specimens. 58.
the
discontinuous ELISA
attempted human.
were
using
T. asiatica or T. saginata adult
against
The sparganum. D. /aturn differentiated from Taenia were
in
by reducing
serum
tapeworm
was
Component
observed to
Wl3~
cestodiases
IgC.
National
of Korea
asiatica
(ELlSA)
(protein
Tricine
raenia
0.210
infections
other
Chungbuk
Republic
blot
assay
antigen
T. asiatica
SDS-PACE
by showing
Medicine,
taeniasis
anti-human
proteins
separating
and
worm
conjugated
using
of
360-763.
of
immunosorbent
peroxidase component
College
Chungbuk
immuncelectrotransfer
differential
enzyme-linked T
225
results
T. asia&
show and
r
that sagin&
the
69 58
differential
infections
by EITB
o-0401 AND PENTASTOMIOSIS IN CHINA PATHOLOGICAL DIAGNOSIS AND II. OF DIFFERENTIAL DIAGNOSIS PENTASTOMIOSIS
PENTASTOMIDS
Rang Yun Lung’ leu Ming Hwa** Ma Kuo Chun’ *Dept. Pathology, Shanghai Medical University. Shanghai, Chrna **Dept. Parasitology, Zhejiang Medical University. Hangzhou, China Nme pathologically studied cases of this rare parasitic drsease collected during 1950’s to 1980’s in Shanghai and Chungqing arc reported. In China we have to emphasize Ihe distmction between the etiopathological diagnosis and presumpuve pathologIca diagnosis of this disease and the sigmficance of its diffcrcntlal diagnons. The reason is that these parasitic pathogens used to be faded away entirely from the lesions in the majority of these cases (6 out of 9). In only 3 cases the precious pathognamonic relics _- the calcified cuticlechtmous oral parts of the pentastomids - had been found in those typical granulomatous lesions, however, the ldenbtication of those characteristic oral parts could only be traced to tbe Armrllr/er sp. Therefore. m most cases, the presumptive pathological diagnoses of ths disease were necessary. They wele made by a series of ‘specific’ pathological characteristics, such as the protuberant ‘pearls’ (sclerotic nodules), the concentric laminations of calcification (targetoid appearance) in a focus of caseous necrosw and the relalively specitic distributional pattern In the differential diagnosis the possibilities of tuberculosis, Pick’s drsease; and the diseases caused by other parasites, such as C~SI~CWCUS cellulose, S’arganum, Echrnococcus granubsus and Diplera larva should be ruled out. To our knowledge the case of pcntaslomiotrc myocarditis might be considered as the first report in the literature The lymphohematogenaus spread of their primary larvae might be responsible for this rntracardlac mvolvemenl.
as