Differential expression of cell adhesion molecules on lung cancer cell lines

12

BIOLOGY

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DIFFERENTIAL EXPRESSION OF CELL ADHESION MOLECULES ON LUNG CANCER CELL LINES

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M.Rotsch. J.Heymanns. C.Hennig, K.Havemann. Dept. of Internal Medicine, Philipps-University,Marhurg,Germany

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The organization of cells in differentiated organs and tissues is depend on cell-surface interactions with molecules on the surface of other cells and with the extracellular matrix. Small cell lu!~:,cancer cell lines (SCLC) express a number of neurors)rjocrine features which non small cell lung cancer cells (NSCLC) genrally lack. The presence of the neuronal adhesion molecule (NCAM) which occurs in nervous tissues and in neuroendocrine tumors has been demonstrated in SCLC cell lines (FEBS-Letters 267,295,1990). Since the identity of the 140kOa isoform of NCAM with the leucocyte differentiation antigen CD56 expressed on natural killer cells has been shown (J.Exp.Med. 1'!(q2?33.1989) we analysed the expression of CD56 (NHK-1 j an2 (l-INK-l: carbohydrate epitope on NCAM)on 19 lung cancer cell lines (7 SCLC classic type, 3 SCLC variant type and 9 NSCLC) by flow cytometry and immunoblot. All SCLC cell lines but one with a substrate adherent growth showed an intense staining with CD56. the coexpression of CD57 varied with two variant lines being negative. Only one large cell lung cancer line was CD56 positive. In contrast to the SCLC the NSCLC showed a staining with CD54 (ICAM). In SCLC lines expression of CD56 correlates with in vitro growth properties and lack of CO57 in the variant subtype indicates differences in glycosylation

vasive

of NCAM. This might be implicated behavior of these lung tumors.

in the in-