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Differential regulation of immediate early genes in the rat PVH in single and repeated stress models
S259
The CRH-SECRETINGCELLSWERE LABELEDWITH FOS AND JUN PROTEINS AFTER I.P. INJECTIONOF ULCERGENICDRUGCINCHOPHEN.HISAKA .TINGU.KIYOSHIGETAKAYAMA \, AND MITSUHIKOMIURA Maebashi. Gunma, 371. Iapan. 2409
In the previous study, we reported that i.p. injection of ulcerogenic cinchophen induced expression of Fos protein in the central autonomic nuclei. The purpose of this study was to obtain evidence that cinchophen induces Fos- and/or Jun-expression in the (XII-secreting cells, because it is certain that CRH is involved in genesis of peptic ulcer. By using double-labeling immunocytochemical technique, we surveyed the distribution of Fos/CRH- and Jun/CRH-labeled cells. These double-labeled cells were located in the paraventricular hypothalamic nucleus (PVH), the central amygdaloid nucleus and the locus coeruleus, but never in the laterodorsal tegmental nucleus and the medial part of the nucleus tractus solitarii. These results indicate that cinchophen induces excitation of CRH-secreting cells in the PVH, and resulting in an increase of ACTH and glucocorticoid.
Differential regulation of immediate early genes in the rat PVH in single and repeated stress models. Santa Umemoto. Yoshinori Kawai. Takashi Uevama and Emiko Senba Dept. of Anatomv and Neurobioloov Wakavama Med. COIL Kvubancho 27 Wakavama 640 Japan.
2410
A single exposure to various kinds of stressors induce immediate early genes (IEGs) such as c-fos, fos 6, jun E, NGFI-A and NGFI-B in discrete brain regions including the hypothalamic paraventricular nucleus (PVH). The PVH which integrates stress response of the hypothalamic-pituitary-adrenal axis contains various kinds of peptides such as corticotropin-releasing factor, vasopressin etc. Previous studies demonstrated that synthesis and release of these peptides are affected by repeated or chronic stress. In this study, using in situ hybridization, we investigated whether the induction of IEGs described above in the PVH are also affected by repeated stress or not. Prior exposure to immobilization (IMO) for 6 days attenuated the induction of all IEGs except NGFI-A mRNA in response to a challenge IMO on the 7 th day. Moreover, sustained elevation of NGFI-A was observed at 24 h after 6 th IMO. These results suggested that repeated stress has different effects on the transcription of NGFI-A and other IEGs in the PVH. In addition, Fos immunostaining combined with in situ hybridization histochemistry for IEG mRNAs revealed concomitant induction of these IEGs and Fos in the same PVH neurons. This indicates that multiple interactions among these IEGs may underlie the present observation.
2411
ANIMALMODELOF DEPRESSION, USINGPRENATALSTRESS.
KATSUKI KITERA’r2, MASAHIKO MIKUNI’, KAZUKO SAITOH’. KATSUMASA MUNEOKA3.CHIHIROYAMAZAKI’, GENRO IKAWA2.KIYOHISA TAKAHASHI’, ‘Dept. of Mental Disorder Research.Natl.lnstiMe of Neuroscience.Natl.Center of Neuroloov and Psvchiatrv,Tokvo,l87,Jaoan, 2Deot. of Psvchiatrv.Facuitv of Medicine.Nara Medical Universttv,Nara534,Jaoan, 3Dept. of Neuropsvchiatrv,Facultv of Medicine,Kaooshima universitv,Kaocshima,Mo,Japan. The present work assessed the effects of prenatal saline injection stress on hypothalamo-pituitaryadrenal (HPA) activity and some behavioral responses of adult offspring. Pregnant rats were given one saline injection (lmllkg, s.c.) daily from gestation day 14 to 20 (Prenatal stress group). Control groups were maintained in no stressful condition. Adult male offspring received various test procedures. The exposure to conditioned fear stress at 8 and 28 weeks of age increased corticosterone secretion to a significantly greater The immobility duration in forced swim test was extent in prenatal stress group than in control group. significantly increased in prenatal stress group at 8 and Plweeks of age. It is well-known that this immobility duration in forced swim test is shortened by the administration of various antidepressant agents. These alterations may be permanent effects of the stressful experience during early life of rats. It is suggested that the prenatal saline injection model does reflect the dysregulation of HPA activity ,which is frequently observed in depression, and may have face validity for the animal model of depression.
The CRH-SECRETINGCELLSWERE LABELEDWITH FOS AND JUN PROTEINS AFTER I.P. INJECTIONOF ULCERGENICDRUGCINCHOPHEN.HISAKA .TINGU.KIYOSHIGETAKAYAMA \, AND MITSUHIKOMIURA Maebashi. Gunma, 371. Iapan. 2409
In the previous study, we reported that i.p. injection of ulcerogenic cinchophen induced expression of Fos protein in the central autonomic nuclei. The purpose of this study was to obtain evidence that cinchophen induces Fos- and/or Jun-expression in the (XII-secreting cells, because it is certain that CRH is involved in genesis of peptic ulcer. By using double-labeling immunocytochemical technique, we surveyed the distribution of Fos/CRH- and Jun/CRH-labeled cells. These double-labeled cells were located in the paraventricular hypothalamic nucleus (PVH), the central amygdaloid nucleus and the locus coeruleus, but never in the laterodorsal tegmental nucleus and the medial part of the nucleus tractus solitarii. These results indicate that cinchophen induces excitation of CRH-secreting cells in the PVH, and resulting in an increase of ACTH and glucocorticoid.
Differential regulation of immediate early genes in the rat PVH in single and repeated stress models. Santa Umemoto. Yoshinori Kawai. Takashi Uevama and Emiko Senba Dept. of Anatomv and Neurobioloov Wakavama Med. COIL Kvubancho 27 Wakavama 640 Japan.
2410
A single exposure to various kinds of stressors induce immediate early genes (IEGs) such as c-fos, fos 6, jun E, NGFI-A and NGFI-B in discrete brain regions including the hypothalamic paraventricular nucleus (PVH). The PVH which integrates stress response of the hypothalamic-pituitary-adrenal axis contains various kinds of peptides such as corticotropin-releasing factor, vasopressin etc. Previous studies demonstrated that synthesis and release of these peptides are affected by repeated or chronic stress. In this study, using in situ hybridization, we investigated whether the induction of IEGs described above in the PVH are also affected by repeated stress or not. Prior exposure to immobilization (IMO) for 6 days attenuated the induction of all IEGs except NGFI-A mRNA in response to a challenge IMO on the 7 th day. Moreover, sustained elevation of NGFI-A was observed at 24 h after 6 th IMO. These results suggested that repeated stress has different effects on the transcription of NGFI-A and other IEGs in the PVH. In addition, Fos immunostaining combined with in situ hybridization histochemistry for IEG mRNAs revealed concomitant induction of these IEGs and Fos in the same PVH neurons. This indicates that multiple interactions among these IEGs may underlie the present observation.
2411
ANIMALMODELOF DEPRESSION, USINGPRENATALSTRESS.
KATSUKI KITERA’r2, MASAHIKO MIKUNI’, KAZUKO SAITOH’. KATSUMASA MUNEOKA3.CHIHIROYAMAZAKI’, GENRO IKAWA2.KIYOHISA TAKAHASHI’, ‘Dept. of Mental Disorder Research.Natl.lnstiMe of Neuroscience.Natl.Center of Neuroloov and Psvchiatrv,Tokvo,l87,Jaoan, 2Deot. of Psvchiatrv.Facuitv of Medicine.Nara Medical Universttv,Nara534,Jaoan, 3Dept. of Neuropsvchiatrv,Facultv of Medicine,Kaooshima universitv,Kaocshima,Mo,Japan. The present work assessed the effects of prenatal saline injection stress on hypothalamo-pituitaryadrenal (HPA) activity and some behavioral responses of adult offspring. Pregnant rats were given one saline injection (lmllkg, s.c.) daily from gestation day 14 to 20 (Prenatal stress group). Control groups were maintained in no stressful condition. Adult male offspring received various test procedures. The exposure to conditioned fear stress at 8 and 28 weeks of age increased corticosterone secretion to a significantly greater The immobility duration in forced swim test was extent in prenatal stress group than in control group. significantly increased in prenatal stress group at 8 and Plweeks of age. It is well-known that this immobility duration in forced swim test is shortened by the administration of various antidepressant agents. These alterations may be permanent effects of the stressful experience during early life of rats. It is suggested that the prenatal saline injection model does reflect the dysregulation of HPA activity ,which is frequently observed in depression, and may have face validity for the animal model of depression.