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Differential white blood cell count and metabolic syndrome in Japanese men
Abstracts / Atherosclerosis 252 (2016) e1ee196
e181
investigate the relationship between LIP and markers of iron metabolism, inflammation, oxidative stress, endothelial dysfunction.
S.Y. Chang, Y.H. Jo, M.J. Kim. College of Medicine- The Catholic University of Korea, Department of Physiology, Seoul, Republic of Korea
Methods: 55 men (24 with a history of acute coronary syndrome > 3 months and 31 healthy controls) underwent physical examination, ultrasound measurement of abdominal subcutaneous fat, and fasting venous blood was drawn. In isolated lympho/monocytes, LIP was measured by spectrofluorimetric method and H-ferritin by Western immunoblotting. Plasma parameters-ferritin, transferrin, hepcidin, oxLDL, anti-oxLDL, ICAM-1, E-selectin, LP-PLA2, TNF-a, MMP-1 by ELISA. Results: The CVD patients showed higher levels of LIP than controls (1.896±0.520 mM vs. 1.593±0.482 mM; p<0.05), though no difference was found in the concentrations of intracellular H-ferritin or plasma total ferritin. The CVD patient group had higher concentrations of ICAM-1 and lower anti-oxLDL, higher waist circumference, intraabdominal fat and parameters of insulin resistance: HOMA-IR, triglycerides than controls. In regression analysis there was a positive correlation of LIP with abdominal fat. Conclusions: Patients with chronic CVD have significantly higher concentrations of LIP in their monocytes than healthy individuals even though the groups did not differ in traditional markers of iron metabolism. LIP positively correlates with abdominal obesity. Supported by the Ministery of Health of the Czech Republic, Grant IGA NT 13671-4/201
Objectives: We previously reported that proinflammatory cytokines induced lipocalin-2 (LCN-2) expression in insulin-producing beta-cells. Of course, inducible nitric oxide synthase (iNOS) induction was observed in the same condition. Therefore, we examined the effect of nitric oxide (NO) on LCN-2 expression in cytokines (interleukin-1b and interferon-g)treated RINm5F beta-cells. Additionally, we observed the effect of LCN-2 on the cell viability.
EAS16-0255, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. PATIENTS WITH RHEUMATOID ARTHRITIS SHOWED ALTERED LIPOPROTEIN PROFILE WITH DYSFUNCTIONAL HIGH-DENSITY LIPOPROTEINS TO EXACERBATES INFLAMMATION AND ATHEROSCLEROTIC PROCESS
Methods: LCN-2 protein and NF-kB activity were observed by Western blot and luciferase assay. LCN-2 mRNA and protein stability assays were performed using actinomycin D chase and cycloheximide chase studies. Cell viability was measured using LCN-2 peptide and expression vector. Results: We found the existence of LCN-2 receptor and the internalization of exogenous recombinant LCN-2 peptide in RINm5F beta-cells. Next, the effects of NO on LCN-2 expression were evaluated. Aminoguanidine, an iNOS inhibitor and iNOS gene silencing significantly inhibited cytokinesinduced LCN-2 expression while sodium nitroprusside, an NO donor potentiated it. Luciferase reporter assay showed that transcription factor NF-kB was not involved in LCN-2 expression. Both LCN-2 mRNA and protein stability assays were conducted. SNP did not affect LCN-2 mRNA stability, however, it significantly reduced LCN-2 protein degradation. The LCN-2 protein degradation was significantly attenuated by MG132, a proteasome inhibitor. Finally, the effect of LCN-2 on cell viability was evaluated. LCN-2 peptide treatment and LCN-2 overexpression significantly reduced cell viability. FACS analysis showed that LCN-2 induced the apoptosis of the cells. Conclusions: The level of NO affects LCN-2 expression via regulation of LCN-2 protein stability under inflammatory condition and LCN-2 may reduce beta-cell viability by promoting apoptosis.
J.Y. Kim 1, 2, 3, E.Y. Lee 1, 2, 3, K.H. Cho 1, 2, 3. 1 Yeungnam University, School of Biotechnology, Gyeongsan, Republic of Korea; 2 Yeungnam University, Research Institute of Protein Sensor, Gyeongsan, Republic of Korea; 3 Yeungnam University, BK21plus Program Serum Biomedical Research and Education Team, Gyeongsan, Republic of Korea
EAS16-0217, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. DIFFERENTIAL WHITE BLOOD CELL COUNT AND METABOLIC SYNDROME IN JAPANESE MEN
Objectives: It has been well established that pro-inflammatory HDL is a biomarker for atherosclerosis in patients with rheumatoid arthritis (RA). Although oxidized LDL is a traditional biomarker of atherosclerosis, there has been many reports that it is also found in RA patients, suggesting lipoprotein metabolism is important in the pathogenesis of RA. In order to investigate a physiological role of lipoprotein in RA. Methods: We analyzed serum lipoproteins from rheumatoid arthritis (RA) both male and female patients. Results: Although they showed normal level of TC, LDL-C, and glucose level RA group showed significant elevation of serum TC, uric acid, CETP activity. LDL in RA group was more oxidized and glycated with more fragmentation of apo-B, suggesting more severe oxidative damage, especially in female group. The LDL was more easily uptaken into macrophage via phagocytosis with more production of oxidized species. HDL from RA group was also showed more glycated and aggregation of apoA-I. With increase of CETP activity and TG contents in lipoproteins, paraoxonase activity was reduced in HDL of RA group. RA group showed elevation of advanced glycated end (AGE) product and severe multimerization of apoAI in HDL. Microinjection of LDL caused severe death of zebrafish embryo in RA group. Under presence of oxidized LDL, HDL3 from RA group lost protectional activity from embryo death around 2-fold higher mortality than control. Conclusions: In conclusion, RA patients showed severely altered lipoprotein profile with dysfunctional HDL, which are very similar properties with that of coronary heart patients.
EAS16-1055, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. THE LEVEL OF NITRIC OXIDE REGULATES LIPOCALIN-2 EXPRESSION UNDER INFLAMMATORY CONDITION IN RINM5F BETA-CELLS
Y. Ikeda 1, N. Hisakawa 1, T. Ohguro 2, J. Nishiuchi 2, Y. Kumon 3. 1 Kochi Memorial Hospital, Diabetes Center, Kochi, Japan; 2 Kochi Red Cross Hospital, Department of Health Examination, Kochi, Japan; 3 Chikamori Hospital, Department of Diabetes and Metabolic Diseases, Kochi, Japan Objectives: White blood cell (WBC) count is a marker of inflammatory conditions. Moderate increases in WBC count have been associated with type 2 diabetes and the progression of atherosclerosis. Low-grade inflammation has been implicated in the pathophysiology of obesityrelated insulin resistance. We examined the associations between differential WBC count and components of metabolic syndrome in Japanese men. Methods: We recruited 283 unrelated Japanese men who underwent health checkups. The population included 179 individuals with normal glucose tolerance, 89 with impaired fasting glucose and/or impaired glucose tolerance, and 15 with diabetes mellitus based on a 75-g oral glucose tolerance test. Sixty-three subjects (22.3%) had metabolic syndrome according to the Japanese diagnostic criteria. Results: Total WBC count of all subjects was positively correlated with body mass index, waist circumference, triglycerides, fasting insulin, homeostasis model assessment of insulin resistance, alanine aminotransferase, uric acid, high-sensitive C-reactive protein, smoking status, and negatively with age, HDL-cholesterol and serum adiponectin concentrations. Total WBC, lymphocyte, neutrophil and monocyte counts were significantly elevated in subjects with metabolic syndrome (p<0.001, <0.001, <0.01 and <0.05, respectively). Among different WBC counts, lymphocytes were associated with serum adiponectin level, independently of high-sensitive C-reactive protein. Multiple regression analysis showed that waist circumference, HDL-cholesterol, triglycerides and smoking status were independently associated with lymphocyte counts.
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Abstracts / Atherosclerosis 252 (2016) e1ee196
Conclusions: In Japanese men, total WBC and lymphocyte counts are significantly associated with components of metabolic syndrome. These counts, in combination with other coronary risk factors, are clinically useful markers for the screening of subjects at high risk of cardiovascular events.
EAS16-0048, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. NON-HDL CHOLESTEROL RELATES TO PRO-INFLAMMATORY STATUS OF HUMAN VISCERAL ADIPOSE TISSUE R. Poledne 1, I. Kralova Lesna 1, A. Kralova 1, A. Sekerkova 2, J. Fronek 3. 1 Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, Prague 4, Czech Republic; 2 Institute for Clinical and Experimental Medicine, Laboratory of Immunology Diagnostic, Prague 4, Czech Republic; 3 Institute for Clinical and Experimental Medicine, Dept. of Transplantation Surgery, Prague 4, Czech Republic Objectives: It has been documented recently that a high fat, high cholesterol diet stimulates the number of macrophages in adipose tissue in experimental models. We analysed the possibility of this relation in detail in healthy men. Methods: Visceral adipose tissue was obtained preoperatively from living kidney donors after organ isolation. The stromal vascular fraction was obtained and purified after incubation of perirenal adipose tissue with collagenase. The number and phenotype of macrophages were determined by flow cytometry. Results: The total number of normally stimulated macrophages correlated positively with non-HDL cholesterol concentration and a significant coefficient of this relation increased gradually with more precise phenotype definition (CD14+, CD16+ and CD36+++; p<0.001). This correlation also remains identical within the normal physiological range when individuals with non-HDL cholesterol concentrations higher than 4 mmol/l were excluded. On the opposite, a significant negative correlation between nonHDL cholesterol and alternatively stimulated macrophages (CD14+, CD16-, CD163+) was documented. Conclusions: There is a positive correlation between physiological concentrations of non-HDL cholesterol and pro-inflammatory macrophages of visceral adipose tissue in men.
EAS16-0148, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. EFFECT OF FAT COMPOSITION OF A MEAL ON THE ACUTE POSTPRANDIAL INFLAMMATORY RESPONSE I. Shapira 1, O. Raz 2, S. Berliner 3. 1 Tel Aviv - Sourasky Medical Center, Directorate, Tel-Aviv, Israel; 2 Tel Aviv - Sourasky Medical Center, Diet Unit, Tel-Aviv, Israel; 3 Tel Aviv - Sourasky Medical Center, Medicine H, Tel-Aviv, Israel Objectives: The presence of low grade, internal inflammation is one of the main causes for development of insulin resistance, type 2 diabetes mellitus and atherosclerosis. Consumption of long lasting diets with various compositions affects the inflammatory response. In this study we investigated the effect of a single meal, composed of different type of fatty acids (saturated; SFA vs. mono-unsaturated fatty acids; MUFA) on the development of inflammation. Methods: Fifty healthy subjects, were randomly chosen to eat two isocaloric meals with similar amounts of fat, either high in SFA or in MUFA, given as breakfast. Following three weeks each participant was given the alternate meal. Blood samples were taken following an overnight fast, and 2, 4 hours following the meal. Results: High SFA meal, but not MUFA increases erythrocyte sedimentation rate, fibrinogen, leukocytes, CRP and IL-6 concentration. In addition, the results indicate that high BMI subjects are less sensitive to the effect of the SFA meal, and the increase in the inflammatory response is more moderate.
Conclusions: A single high SFA meal induces immediate postprandial inflammatory response. This study emphasizes the importance of consuming diet with anti-inflammatory effects, showing the harm influence of a single unfavorable meal.
EAS16-0151, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. THE RELATIONSHIP BETWEEN HELICOBACTER PYLORI INFECTION AND ARTERIAL STIFFNESS IN ADULTS WITH ABNORMAL GASTRIC ENDOSCOPIC FINDINGS K.C. Park 1, M.J. Kim 2, K.S. Park 2, Y.S. Kim 2, H.C. Cho 3, H.R. Sung 4, K.H. Park 4, B.H. Choi 5, K.C. Kim 5. 1 Bundang CHA Medical Center- CHA University, Family Medicine, Seongnam-si, Republic of Korea; 2 Bundang CHA Medical Center- CHA university, Department of Family medicine, Seong-nam si, Republic of Korea; 3 Bungdang CHA Medical Center, Family Medicine, Seong-nam si, Republic of Korea; 4 Bungdang CHA Medical Center, Family Medicine, Seong-Nam, Republic of Korea; 5 CHA Gangnam Medical Center, Family medicine, Seoul, Republic of Korea Objectives: The correlation between Helicobacter pylori (H.pylori) infection and atherosclerosis remains controversial. The purpose of this study was to determine the relationship between H.pylori infection and arterial stiffness assessed by measuring brachial-ankle pulse wave velocity (baPWV), Sbeing clinically used as a marker of atherosclerosis in adults with abnormal gastric endoscopic findings. Methods: The subjects were 1119 Korean adults with abnormal gastric endoscopic findings who have a check-up between January 2011 and June 2013. H.pylori infection status was confirmed by endoscopic biopsy. Systolic blood pressure(SBP), heart rate(HR), baPWV, fasting blood glucose(FBG), lipid profile and other traditional risk factors for cardiovascular diseases were measured. The data for H.pylori infectees and H.pylori non-infectees were compared. The relationship between H.pylori infection and arterial stiffness was investigated by multiple linear regression analysis. Results: four hundred and seventy-five subjects(42.45%) were H.pylori infectees. Male(70.3% vs 61%, p¼0.001), smoking(41.8% vs 29.8%, p<0.001) and Low density lipoprotein cholesterol( 117.3±28.5 vs 113.2±29.3, p¼0.018) were significantly higher in H. pylori infectees than in H. pylori non-infectees. baPWV was significantly higher in H. pylori infectees than in H. pylori non-infectees(1355.7±224 vs. 1329.6±214.9 cm/s, p¼0.049). Multiple regression analysis indicated that H.pylori infection(b¼19.31, P¼0.032) was significantly positive correlation with baPWV after adjustment for age, BMI and conventional risk factors of cardiovascular diseases, including SBP, ABI, HR, total cholesterol, FBG, smoking and alcohol drinking. Conclusions: These results suggest that H.pylori infection in patients with abnormal gastric enoscopic finding such as gastritis, erosion, ulcer and polyp is a potential risk factor for increased baPWV levels.
EAS16-0558, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. PROTECTIVE EFFECT OF CAROTENOIDS ON CX40 AD CX43 EXPRESSION IN THE LEFT VENTRICLE OF NORMOTENSIVE WISTAR RATS AFTER ADMINISTRATION OF LPS K. Frimmel 1, J. Krizak 1, B. Liptak 2, J. Navarova 2, V. Knezl 2, E. Breierova 3, I. Bernatova 4, L. Okruhlicova 1. 1 Institute for Hear Research Slovak Academy of Sciences, Institute for Heart Research, Bratislava, Slovakia; 2 Institute of Experimental Pharmacology & Toxicology Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Bratislava, Slovakia; 3 Institute of Chemistry Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovakia; 4 Institute of Normal and Pathological Physiology Slovak Academy of Sciences, Institute of Normal and Pathological Physiology, Bratislava, Slovakia Objectives: The aim of our pilot study was to examine the effect of LPS (bacterial endotoxin) on expression of Cx43 (mainly myocardial) and Cx40
e181
investigate the relationship between LIP and markers of iron metabolism, inflammation, oxidative stress, endothelial dysfunction.
S.Y. Chang, Y.H. Jo, M.J. Kim. College of Medicine- The Catholic University of Korea, Department of Physiology, Seoul, Republic of Korea
Methods: 55 men (24 with a history of acute coronary syndrome > 3 months and 31 healthy controls) underwent physical examination, ultrasound measurement of abdominal subcutaneous fat, and fasting venous blood was drawn. In isolated lympho/monocytes, LIP was measured by spectrofluorimetric method and H-ferritin by Western immunoblotting. Plasma parameters-ferritin, transferrin, hepcidin, oxLDL, anti-oxLDL, ICAM-1, E-selectin, LP-PLA2, TNF-a, MMP-1 by ELISA. Results: The CVD patients showed higher levels of LIP than controls (1.896±0.520 mM vs. 1.593±0.482 mM; p<0.05), though no difference was found in the concentrations of intracellular H-ferritin or plasma total ferritin. The CVD patient group had higher concentrations of ICAM-1 and lower anti-oxLDL, higher waist circumference, intraabdominal fat and parameters of insulin resistance: HOMA-IR, triglycerides than controls. In regression analysis there was a positive correlation of LIP with abdominal fat. Conclusions: Patients with chronic CVD have significantly higher concentrations of LIP in their monocytes than healthy individuals even though the groups did not differ in traditional markers of iron metabolism. LIP positively correlates with abdominal obesity. Supported by the Ministery of Health of the Czech Republic, Grant IGA NT 13671-4/201
Objectives: We previously reported that proinflammatory cytokines induced lipocalin-2 (LCN-2) expression in insulin-producing beta-cells. Of course, inducible nitric oxide synthase (iNOS) induction was observed in the same condition. Therefore, we examined the effect of nitric oxide (NO) on LCN-2 expression in cytokines (interleukin-1b and interferon-g)treated RINm5F beta-cells. Additionally, we observed the effect of LCN-2 on the cell viability.
EAS16-0255, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. PATIENTS WITH RHEUMATOID ARTHRITIS SHOWED ALTERED LIPOPROTEIN PROFILE WITH DYSFUNCTIONAL HIGH-DENSITY LIPOPROTEINS TO EXACERBATES INFLAMMATION AND ATHEROSCLEROTIC PROCESS
Methods: LCN-2 protein and NF-kB activity were observed by Western blot and luciferase assay. LCN-2 mRNA and protein stability assays were performed using actinomycin D chase and cycloheximide chase studies. Cell viability was measured using LCN-2 peptide and expression vector. Results: We found the existence of LCN-2 receptor and the internalization of exogenous recombinant LCN-2 peptide in RINm5F beta-cells. Next, the effects of NO on LCN-2 expression were evaluated. Aminoguanidine, an iNOS inhibitor and iNOS gene silencing significantly inhibited cytokinesinduced LCN-2 expression while sodium nitroprusside, an NO donor potentiated it. Luciferase reporter assay showed that transcription factor NF-kB was not involved in LCN-2 expression. Both LCN-2 mRNA and protein stability assays were conducted. SNP did not affect LCN-2 mRNA stability, however, it significantly reduced LCN-2 protein degradation. The LCN-2 protein degradation was significantly attenuated by MG132, a proteasome inhibitor. Finally, the effect of LCN-2 on cell viability was evaluated. LCN-2 peptide treatment and LCN-2 overexpression significantly reduced cell viability. FACS analysis showed that LCN-2 induced the apoptosis of the cells. Conclusions: The level of NO affects LCN-2 expression via regulation of LCN-2 protein stability under inflammatory condition and LCN-2 may reduce beta-cell viability by promoting apoptosis.
J.Y. Kim 1, 2, 3, E.Y. Lee 1, 2, 3, K.H. Cho 1, 2, 3. 1 Yeungnam University, School of Biotechnology, Gyeongsan, Republic of Korea; 2 Yeungnam University, Research Institute of Protein Sensor, Gyeongsan, Republic of Korea; 3 Yeungnam University, BK21plus Program Serum Biomedical Research and Education Team, Gyeongsan, Republic of Korea
EAS16-0217, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. DIFFERENTIAL WHITE BLOOD CELL COUNT AND METABOLIC SYNDROME IN JAPANESE MEN
Objectives: It has been well established that pro-inflammatory HDL is a biomarker for atherosclerosis in patients with rheumatoid arthritis (RA). Although oxidized LDL is a traditional biomarker of atherosclerosis, there has been many reports that it is also found in RA patients, suggesting lipoprotein metabolism is important in the pathogenesis of RA. In order to investigate a physiological role of lipoprotein in RA. Methods: We analyzed serum lipoproteins from rheumatoid arthritis (RA) both male and female patients. Results: Although they showed normal level of TC, LDL-C, and glucose level RA group showed significant elevation of serum TC, uric acid, CETP activity. LDL in RA group was more oxidized and glycated with more fragmentation of apo-B, suggesting more severe oxidative damage, especially in female group. The LDL was more easily uptaken into macrophage via phagocytosis with more production of oxidized species. HDL from RA group was also showed more glycated and aggregation of apoA-I. With increase of CETP activity and TG contents in lipoproteins, paraoxonase activity was reduced in HDL of RA group. RA group showed elevation of advanced glycated end (AGE) product and severe multimerization of apoAI in HDL. Microinjection of LDL caused severe death of zebrafish embryo in RA group. Under presence of oxidized LDL, HDL3 from RA group lost protectional activity from embryo death around 2-fold higher mortality than control. Conclusions: In conclusion, RA patients showed severely altered lipoprotein profile with dysfunctional HDL, which are very similar properties with that of coronary heart patients.
EAS16-1055, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. THE LEVEL OF NITRIC OXIDE REGULATES LIPOCALIN-2 EXPRESSION UNDER INFLAMMATORY CONDITION IN RINM5F BETA-CELLS
Y. Ikeda 1, N. Hisakawa 1, T. Ohguro 2, J. Nishiuchi 2, Y. Kumon 3. 1 Kochi Memorial Hospital, Diabetes Center, Kochi, Japan; 2 Kochi Red Cross Hospital, Department of Health Examination, Kochi, Japan; 3 Chikamori Hospital, Department of Diabetes and Metabolic Diseases, Kochi, Japan Objectives: White blood cell (WBC) count is a marker of inflammatory conditions. Moderate increases in WBC count have been associated with type 2 diabetes and the progression of atherosclerosis. Low-grade inflammation has been implicated in the pathophysiology of obesityrelated insulin resistance. We examined the associations between differential WBC count and components of metabolic syndrome in Japanese men. Methods: We recruited 283 unrelated Japanese men who underwent health checkups. The population included 179 individuals with normal glucose tolerance, 89 with impaired fasting glucose and/or impaired glucose tolerance, and 15 with diabetes mellitus based on a 75-g oral glucose tolerance test. Sixty-three subjects (22.3%) had metabolic syndrome according to the Japanese diagnostic criteria. Results: Total WBC count of all subjects was positively correlated with body mass index, waist circumference, triglycerides, fasting insulin, homeostasis model assessment of insulin resistance, alanine aminotransferase, uric acid, high-sensitive C-reactive protein, smoking status, and negatively with age, HDL-cholesterol and serum adiponectin concentrations. Total WBC, lymphocyte, neutrophil and monocyte counts were significantly elevated in subjects with metabolic syndrome (p<0.001, <0.001, <0.01 and <0.05, respectively). Among different WBC counts, lymphocytes were associated with serum adiponectin level, independently of high-sensitive C-reactive protein. Multiple regression analysis showed that waist circumference, HDL-cholesterol, triglycerides and smoking status were independently associated with lymphocyte counts.
e182
Abstracts / Atherosclerosis 252 (2016) e1ee196
Conclusions: In Japanese men, total WBC and lymphocyte counts are significantly associated with components of metabolic syndrome. These counts, in combination with other coronary risk factors, are clinically useful markers for the screening of subjects at high risk of cardiovascular events.
EAS16-0048, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. NON-HDL CHOLESTEROL RELATES TO PRO-INFLAMMATORY STATUS OF HUMAN VISCERAL ADIPOSE TISSUE R. Poledne 1, I. Kralova Lesna 1, A. Kralova 1, A. Sekerkova 2, J. Fronek 3. 1 Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, Prague 4, Czech Republic; 2 Institute for Clinical and Experimental Medicine, Laboratory of Immunology Diagnostic, Prague 4, Czech Republic; 3 Institute for Clinical and Experimental Medicine, Dept. of Transplantation Surgery, Prague 4, Czech Republic Objectives: It has been documented recently that a high fat, high cholesterol diet stimulates the number of macrophages in adipose tissue in experimental models. We analysed the possibility of this relation in detail in healthy men. Methods: Visceral adipose tissue was obtained preoperatively from living kidney donors after organ isolation. The stromal vascular fraction was obtained and purified after incubation of perirenal adipose tissue with collagenase. The number and phenotype of macrophages were determined by flow cytometry. Results: The total number of normally stimulated macrophages correlated positively with non-HDL cholesterol concentration and a significant coefficient of this relation increased gradually with more precise phenotype definition (CD14+, CD16+ and CD36+++; p<0.001). This correlation also remains identical within the normal physiological range when individuals with non-HDL cholesterol concentrations higher than 4 mmol/l were excluded. On the opposite, a significant negative correlation between nonHDL cholesterol and alternatively stimulated macrophages (CD14+, CD16-, CD163+) was documented. Conclusions: There is a positive correlation between physiological concentrations of non-HDL cholesterol and pro-inflammatory macrophages of visceral adipose tissue in men.
EAS16-0148, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. EFFECT OF FAT COMPOSITION OF A MEAL ON THE ACUTE POSTPRANDIAL INFLAMMATORY RESPONSE I. Shapira 1, O. Raz 2, S. Berliner 3. 1 Tel Aviv - Sourasky Medical Center, Directorate, Tel-Aviv, Israel; 2 Tel Aviv - Sourasky Medical Center, Diet Unit, Tel-Aviv, Israel; 3 Tel Aviv - Sourasky Medical Center, Medicine H, Tel-Aviv, Israel Objectives: The presence of low grade, internal inflammation is one of the main causes for development of insulin resistance, type 2 diabetes mellitus and atherosclerosis. Consumption of long lasting diets with various compositions affects the inflammatory response. In this study we investigated the effect of a single meal, composed of different type of fatty acids (saturated; SFA vs. mono-unsaturated fatty acids; MUFA) on the development of inflammation. Methods: Fifty healthy subjects, were randomly chosen to eat two isocaloric meals with similar amounts of fat, either high in SFA or in MUFA, given as breakfast. Following three weeks each participant was given the alternate meal. Blood samples were taken following an overnight fast, and 2, 4 hours following the meal. Results: High SFA meal, but not MUFA increases erythrocyte sedimentation rate, fibrinogen, leukocytes, CRP and IL-6 concentration. In addition, the results indicate that high BMI subjects are less sensitive to the effect of the SFA meal, and the increase in the inflammatory response is more moderate.
Conclusions: A single high SFA meal induces immediate postprandial inflammatory response. This study emphasizes the importance of consuming diet with anti-inflammatory effects, showing the harm influence of a single unfavorable meal.
EAS16-0151, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. THE RELATIONSHIP BETWEEN HELICOBACTER PYLORI INFECTION AND ARTERIAL STIFFNESS IN ADULTS WITH ABNORMAL GASTRIC ENDOSCOPIC FINDINGS K.C. Park 1, M.J. Kim 2, K.S. Park 2, Y.S. Kim 2, H.C. Cho 3, H.R. Sung 4, K.H. Park 4, B.H. Choi 5, K.C. Kim 5. 1 Bundang CHA Medical Center- CHA University, Family Medicine, Seongnam-si, Republic of Korea; 2 Bundang CHA Medical Center- CHA university, Department of Family medicine, Seong-nam si, Republic of Korea; 3 Bungdang CHA Medical Center, Family Medicine, Seong-nam si, Republic of Korea; 4 Bungdang CHA Medical Center, Family Medicine, Seong-Nam, Republic of Korea; 5 CHA Gangnam Medical Center, Family medicine, Seoul, Republic of Korea Objectives: The correlation between Helicobacter pylori (H.pylori) infection and atherosclerosis remains controversial. The purpose of this study was to determine the relationship between H.pylori infection and arterial stiffness assessed by measuring brachial-ankle pulse wave velocity (baPWV), Sbeing clinically used as a marker of atherosclerosis in adults with abnormal gastric endoscopic findings. Methods: The subjects were 1119 Korean adults with abnormal gastric endoscopic findings who have a check-up between January 2011 and June 2013. H.pylori infection status was confirmed by endoscopic biopsy. Systolic blood pressure(SBP), heart rate(HR), baPWV, fasting blood glucose(FBG), lipid profile and other traditional risk factors for cardiovascular diseases were measured. The data for H.pylori infectees and H.pylori non-infectees were compared. The relationship between H.pylori infection and arterial stiffness was investigated by multiple linear regression analysis. Results: four hundred and seventy-five subjects(42.45%) were H.pylori infectees. Male(70.3% vs 61%, p¼0.001), smoking(41.8% vs 29.8%, p<0.001) and Low density lipoprotein cholesterol( 117.3±28.5 vs 113.2±29.3, p¼0.018) were significantly higher in H. pylori infectees than in H. pylori non-infectees. baPWV was significantly higher in H. pylori infectees than in H. pylori non-infectees(1355.7±224 vs. 1329.6±214.9 cm/s, p¼0.049). Multiple regression analysis indicated that H.pylori infection(b¼19.31, P¼0.032) was significantly positive correlation with baPWV after adjustment for age, BMI and conventional risk factors of cardiovascular diseases, including SBP, ABI, HR, total cholesterol, FBG, smoking and alcohol drinking. Conclusions: These results suggest that H.pylori infection in patients with abnormal gastric enoscopic finding such as gastritis, erosion, ulcer and polyp is a potential risk factor for increased baPWV levels.
EAS16-0558, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. PROTECTIVE EFFECT OF CAROTENOIDS ON CX40 AD CX43 EXPRESSION IN THE LEFT VENTRICLE OF NORMOTENSIVE WISTAR RATS AFTER ADMINISTRATION OF LPS K. Frimmel 1, J. Krizak 1, B. Liptak 2, J. Navarova 2, V. Knezl 2, E. Breierova 3, I. Bernatova 4, L. Okruhlicova 1. 1 Institute for Hear Research Slovak Academy of Sciences, Institute for Heart Research, Bratislava, Slovakia; 2 Institute of Experimental Pharmacology & Toxicology Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Bratislava, Slovakia; 3 Institute of Chemistry Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovakia; 4 Institute of Normal and Pathological Physiology Slovak Academy of Sciences, Institute of Normal and Pathological Physiology, Bratislava, Slovakia Objectives: The aim of our pilot study was to examine the effect of LPS (bacterial endotoxin) on expression of Cx43 (mainly myocardial) and Cx40