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Direct Regulation of tRNA and 5S rRNA Gene Transcription by Polo-like Kinase 1
Molecular Cell
Errata Direct Regulation of tRNA and 5S rRNA Gene Transcription by Polo-like Kinase 1 Jennifer A. Fairley,1,2 Louise E. Mitchell,1,2 Tracy Berg,1 Niall S. Kenneth,2 Conrad von Schubert,3 Herman H.W. Sillje´,4 Rene´ H. Medema,5 Erich A. Nigg,3 and Robert J. White1,2,* 1Beatson
Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK 3Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056 Basel, Switzerland 4Department of Cardiology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The Netherlands 5Department of Medical Oncology, University Medical Center, 3584 CG Utrecht, The Netherlands *Correspondence: [email protected] http://dx.doi.org/10.1016/j.molcel.2012.06.022 2Institute
(Molecular Cell 45, 541–552; Published online January 26, 2012) In the above article, inadvertent errors were included in Figures 5E and 6D. In Figure 5E, one of the original FACS profiles was a duplication of another. In Figure 6D, the second panel was a shorter exposure of the top panel. We have reproduced the experiments, and new versions of the figures are included below. None of the conclusions of this article are altered. We apologize for these errors. Figure 5. T270 of Brf1 Is Phosphorylated When Plk1 Becomes Activated
A Brf1 P T270 Brf1 P S450 Plk1 Plk1 P T210 H3 P Ser10 TFIIB
Cell cycle phase: Plk1 inhibitor:
S -
Control siRNA
C M +
-
+
*
Plk1 siRNA 2
B
Plk1 siRNA 1
Cell cycle profiles
Brf1 P T270
Plk1
Brf1
Brf1 P T270 Brf1
TFIIB
Actin
D WT Plk1 Plk1 Induction: +
T210D Plk1 +
E
WT Plk1
Brf1 P S450 Western
Brf1 P T270
Uninduced
Total Brf1 Plk1 Actin tRNA
RT-PCR
Plk1 Induced
ARPP P0 mRNA
148 Molecular Cell 47, 148–150, July 13, 2012 ª2012 Elsevier Inc.
T210D Plk1
Molecular Cell
T270D Brf1 T270D Brf1
Figure 6. T270 of Brf1 Mediates Repression by Plk1 of Pol III Binding and Transcription
1.5
tRNA
Relative 1 tRNA Expression 0.5
5S rRNA
RT-PCR
T270A Brf1
2
Vector
Transfected:
WT Brf1
B
T270A Brf1
T270D Brf1
Transfected:
T270A Brf1
Vector
A
WT Brf1
Errata
0
Vector
C HA-Brf1
Transfected:
Western Actin
WT Brf1
TFIIB mRNA
IP Pol III WB: HA
D
WB: HA
Transfected:
Vector
WT Brf1
Plk1 T210D:
-
-
+
T270A Brf1
+
-
WB: Actin
+
tRNA
F
tRNA 5S rRNA
5S rRNA
RT-PCR
ARPP P0 mRNA
Pol III Plk1
HA-Brf1
TFIIIB
Plk1
Western
S450 P
Actin
TFIIIC
Interphase
E Transfected:
Vector
Plk1 T210D: 2
Relative 1.5 tRNA Expression 1 0.5
+
WT Brf1 -
+
T270A Brf1 -
+
T210 P Plk1 T270 P
TFIIIB
S450 P TFIIIC
0
Mitosis
Molecular Cell 47, 148–150, July 13, 2012 ª2012 Elsevier Inc. 149
Errata Direct Regulation of tRNA and 5S rRNA Gene Transcription by Polo-like Kinase 1 Jennifer A. Fairley,1,2 Louise E. Mitchell,1,2 Tracy Berg,1 Niall S. Kenneth,2 Conrad von Schubert,3 Herman H.W. Sillje´,4 Rene´ H. Medema,5 Erich A. Nigg,3 and Robert J. White1,2,* 1Beatson
Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK 3Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056 Basel, Switzerland 4Department of Cardiology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The Netherlands 5Department of Medical Oncology, University Medical Center, 3584 CG Utrecht, The Netherlands *Correspondence: [email protected] http://dx.doi.org/10.1016/j.molcel.2012.06.022 2Institute
(Molecular Cell 45, 541–552; Published online January 26, 2012) In the above article, inadvertent errors were included in Figures 5E and 6D. In Figure 5E, one of the original FACS profiles was a duplication of another. In Figure 6D, the second panel was a shorter exposure of the top panel. We have reproduced the experiments, and new versions of the figures are included below. None of the conclusions of this article are altered. We apologize for these errors. Figure 5. T270 of Brf1 Is Phosphorylated When Plk1 Becomes Activated
A Brf1 P T270 Brf1 P S450 Plk1 Plk1 P T210 H3 P Ser10 TFIIB
Cell cycle phase: Plk1 inhibitor:
S -
Control siRNA
C M +
-
+
*
Plk1 siRNA 2
B
Plk1 siRNA 1
Cell cycle profiles
Brf1 P T270
Plk1
Brf1
Brf1 P T270 Brf1
TFIIB
Actin
D WT Plk1 Plk1 Induction: +
T210D Plk1 +
E
WT Plk1
Brf1 P S450 Western
Brf1 P T270
Uninduced
Total Brf1 Plk1 Actin tRNA
RT-PCR
Plk1 Induced
ARPP P0 mRNA
148 Molecular Cell 47, 148–150, July 13, 2012 ª2012 Elsevier Inc.
T210D Plk1
Molecular Cell
T270D Brf1 T270D Brf1
Figure 6. T270 of Brf1 Mediates Repression by Plk1 of Pol III Binding and Transcription
1.5
tRNA
Relative 1 tRNA Expression 0.5
5S rRNA
RT-PCR
T270A Brf1
2
Vector
Transfected:
WT Brf1
B
T270A Brf1
T270D Brf1
Transfected:
T270A Brf1
Vector
A
WT Brf1
Errata
0
Vector
C HA-Brf1
Transfected:
Western Actin
WT Brf1
TFIIB mRNA
IP Pol III WB: HA
D
WB: HA
Transfected:
Vector
WT Brf1
Plk1 T210D:
-
-
+
T270A Brf1
+
-
WB: Actin
+
tRNA
F
tRNA 5S rRNA
5S rRNA
RT-PCR
ARPP P0 mRNA
Pol III Plk1
HA-Brf1
TFIIIB
Plk1
Western
S450 P
Actin
TFIIIC
Interphase
E Transfected:
Vector
Plk1 T210D: 2
Relative 1.5 tRNA Expression 1 0.5
+
WT Brf1 -
+
T270A Brf1 -
+
T210 P Plk1 T270 P
TFIIIB
S450 P TFIIIC
0
Mitosis
Molecular Cell 47, 148–150, July 13, 2012 ª2012 Elsevier Inc. 149