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Distribution of mRNA encoding SCG10 family (SCG10, RB3, and SCLIP) in rat brain
S129
A GENE FOR PNUT/SEPTIN-LIKE
201
IN
SHIGENOBU
RAT
ADULT
TODA’ ‘, YASUSHI
ASAMJ UMINO’,
PROTEIN ALTERNATIVELY
EXPRESSED
BRAIN KAJII’, KOYUKI KANEDA’,
MITUMOTO SAT@, TORU NISHIKAWA’
‘Mental Disorder Research, ‘Dept. of Psychiatry,
National Institute of Neuroscience,
Tohoku Univ. School of Medicine,
NCNP, 4-1-l Ogawahigashi,
l-1 Seiryo, Aoba-ku,
Sendai-city
Kodaira-city,
Tokyo 187.8_w2
980-0872
To identify genes contributing
to developmental
changes of brain function, we have performed
compare resulting fingerprints
of rat neocortex.
A cDNA band that was specific to postnatal days (PD) SO compared with
arbitrarily primed PCR to
PD 8 rat was cloned and shown to be a gene fragment with significant homology to genes of septin family. The full-length structure obtained by rapid amplification of cDNA ends revealed that 89% of nucleic acids, and 98% of amino acids were identical to those of human CDC rel-l/ PNUTLI . There are three variants of transcripts with different seemed to encode proteins around 370 amino acids with different N-termini. Three polyadenylation
5’ end and they
recognition
sequences
were followed by each particular poly (A) tail. As reported in the human case, the longest transcript including the another adjacent 3’ gene was also identified.
202 HIROSHI
DISTRIBUTION
OF mRNA ENCODING
SCGlO FAMILY (SCGIO, RB3, AND SCLIP) IN RAT BRAIN
MORII’~*, NOZOMU MORI’,’
‘CREST, JRDC, ‘Department of Molecular Genetics, NILS, Obu-shi, Aichi 474-8522
The neuron-specific is dominantly maturation.
protein SCGIO with a M W. of about 20 kDa, is highly expressed
localized
in the neuronal
that SCGlO may work as a catastrophic been identified
as new members
remain high even in the adults. differences
growth
cone.
Thereafter,
SCGlO play an important role in the neurite outgrowth
in physiological
specific riboprobes
factor for microtubule
of the SCGlO Although
the predicted
for SCGIO family members
203
ABNORMAL
protein structures
and investigated
BRAIN DEVELOPMENT
YOSUKE TAKEI, TENG JUNLW. AND NOBUTAKA HIROKAWA Dept. of Cell Biology and Anatomy,
AKlHIRO
in the neurites.
decreased
of tubulin,
Recently,
and
with the suggesting
RB3 and SCLIP have
In contrast to SCGIO, mRNA levels for RB3 and SCLIP for those three genes
among SCGIO family members
SCGlO family members in rat brain using in situ hybridization
level is gradually
by inducing the depolymerization
dynamics
gene family.
function and distribution
the expression
during the neuronal development
are highly
conserved,
remain unknown.
We here prepared
the temporal and regional distribution
of mRNA encoding
technique.
IN MICE LACKING TAU AND MAPIB
HARADA,
SATOMI INOMATA-TERADA,
Graduate School of Medicine, Univ. of Tokyo. Tokyo I 13-0033. JAPAN
Tau and Microtubule-associated protein 1B (MAPlB) are main members of the microtubule associated proteins (MAPS), which are major components of the neuronal cytoskeleton. Lines of evidences indicate that they play crucial roles in neuronal morphogenesis and neurite extension. However, in our previous efforts, mutant mice homozygous for disrupted tau or MAPlB gene exhibited relatively mild phenotypes (Harada et al. 1994. Nature 369: 488-491; and Takei et al. 1997. J. Cell Biol. 137: 1615-1626). To better define the role of these MAPS it1 viva, we have generated double mutants lacking both tau and MAPlB. We found severe brain abnormalities including defects of corpus callosum in double mutants, which indicates that tau and MAPIB act coopelatively in neuronal morphogenesis and a lack of both MAPS results in the severe impairment of axonal elongation.
A GENE FOR PNUT/SEPTIN-LIKE
201
IN
SHIGENOBU
RAT
ADULT
TODA’ ‘, YASUSHI
ASAMJ UMINO’,
PROTEIN ALTERNATIVELY
EXPRESSED
BRAIN KAJII’, KOYUKI KANEDA’,
MITUMOTO SAT@, TORU NISHIKAWA’
‘Mental Disorder Research, ‘Dept. of Psychiatry,
National Institute of Neuroscience,
Tohoku Univ. School of Medicine,
NCNP, 4-1-l Ogawahigashi,
l-1 Seiryo, Aoba-ku,
Sendai-city
Kodaira-city,
Tokyo 187.8_w2
980-0872
To identify genes contributing
to developmental
changes of brain function, we have performed
compare resulting fingerprints
of rat neocortex.
A cDNA band that was specific to postnatal days (PD) SO compared with
arbitrarily primed PCR to
PD 8 rat was cloned and shown to be a gene fragment with significant homology to genes of septin family. The full-length structure obtained by rapid amplification of cDNA ends revealed that 89% of nucleic acids, and 98% of amino acids were identical to those of human CDC rel-l/ PNUTLI . There are three variants of transcripts with different seemed to encode proteins around 370 amino acids with different N-termini. Three polyadenylation
5’ end and they
recognition
sequences
were followed by each particular poly (A) tail. As reported in the human case, the longest transcript including the another adjacent 3’ gene was also identified.
202 HIROSHI
DISTRIBUTION
OF mRNA ENCODING
SCGlO FAMILY (SCGIO, RB3, AND SCLIP) IN RAT BRAIN
MORII’~*, NOZOMU MORI’,’
‘CREST, JRDC, ‘Department of Molecular Genetics, NILS, Obu-shi, Aichi 474-8522
The neuron-specific is dominantly maturation.
protein SCGIO with a M W. of about 20 kDa, is highly expressed
localized
in the neuronal
that SCGlO may work as a catastrophic been identified
as new members
remain high even in the adults. differences
growth
cone.
Thereafter,
SCGlO play an important role in the neurite outgrowth
in physiological
specific riboprobes
factor for microtubule
of the SCGlO Although
the predicted
for SCGIO family members
203
ABNORMAL
protein structures
and investigated
BRAIN DEVELOPMENT
YOSUKE TAKEI, TENG JUNLW. AND NOBUTAKA HIROKAWA Dept. of Cell Biology and Anatomy,
AKlHIRO
in the neurites.
decreased
of tubulin,
Recently,
and
with the suggesting
RB3 and SCLIP have
In contrast to SCGIO, mRNA levels for RB3 and SCLIP for those three genes
among SCGIO family members
SCGlO family members in rat brain using in situ hybridization
level is gradually
by inducing the depolymerization
dynamics
gene family.
function and distribution
the expression
during the neuronal development
are highly
conserved,
remain unknown.
We here prepared
the temporal and regional distribution
of mRNA encoding
technique.
IN MICE LACKING TAU AND MAPIB
HARADA,
SATOMI INOMATA-TERADA,
Graduate School of Medicine, Univ. of Tokyo. Tokyo I 13-0033. JAPAN
Tau and Microtubule-associated protein 1B (MAPlB) are main members of the microtubule associated proteins (MAPS), which are major components of the neuronal cytoskeleton. Lines of evidences indicate that they play crucial roles in neuronal morphogenesis and neurite extension. However, in our previous efforts, mutant mice homozygous for disrupted tau or MAPlB gene exhibited relatively mild phenotypes (Harada et al. 1994. Nature 369: 488-491; and Takei et al. 1997. J. Cell Biol. 137: 1615-1626). To better define the role of these MAPS it1 viva, we have generated double mutants lacking both tau and MAPlB. We found severe brain abnormalities including defects of corpus callosum in double mutants, which indicates that tau and MAPIB act coopelatively in neuronal morphogenesis and a lack of both MAPS results in the severe impairment of axonal elongation.