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Disturbed carnitine metabolism in hemodialysis (HD) patients?
P.77 Effect
of carnitine on regeneration of the Z. Zadak, J. %mek, M. Hole&k, L. Sobotka University, Hradec Kralove, Czechoslovakia).
liver after partial hepatectomy in rats. (2nd Department of Intern. Med., Charles
The decrease in carnitine following severe liver damage and literary data suggesting that the liver tissue obtains energy primarily through FFA oxidation leads to the question how the administration of carnitine influences regeneration of the liver after partial hepatectomy (PH). Male rats of the Wistar strain were injected i.p. carnitinein dosesof 5 mg, 10 mg and 20 mg/kg body mass immediately and 6 hours after PH. Control rats were administered physiological saline. The course of liver regeneration was assessed on the basis of changes in specific DNA activity and of total DNA content in the liver 18 and 24 hours after PH. Eighteen hours after PH, i.e. during the onset of DNA synthesis, the values of specific DNA activity in rats following the administration of carnitine were significantly higher as compared with the control group (p< 0.01). Twenty-four hours after PH, this change induced an increase in total DNA in the liver of rats injected with carin the content of DNA depended nitine in comparison to the control group. The increase on the magnitude of the dose of carnitine administered (pd 0.05). Twenty-four hours after administration of carnitine, specific activity of DNA in the liver was significantly lower as compared with the controls (p< 0.05). The increase in specific act. of DNA in the liver 18 hours after PH, the decrease in the activity after 24 hours and the statistically significant increase in total DNA in the liver after 24 hours, as compared with the control group, suggest accelerated onset of liver regeneration after administration of carnitine.
IN HJXODIALYSIS(HD) PATIENTS?C.RGssle(l),K.P.Kohse(2) P.78 DISTURBEDCARNITINEMETABOLISM
W.Kapp(Z),H.-E.Franz(Z), A.GlGggler(l ),J.Bergstr&n(31 and P.Fiim (1) Inst. for Biol. Chem. and Nutr., Univ. of Hohenheim, Stuttgart, FRG; (2) Dept. of Nephrol., Univ. Hospital Ulm, FRG; (3) Dept. of Renal Medicine, Univ. Hospital Huddinge, Sweden.
An increase in the ratio of acylcarnitines (AC) to free camitine (FC) is a commonobservatron m HD-patients. The present study was designed to evaluate whether this abnormality is due to 1) extensive losses of FC through the dialysis fluid, 2) an incomplete oxidation of free fatty acids own to a total camitine (TC) deficiency in muscle or 3) a restricted mitochondrial transport of AC. In healthy controls and long-term HD-patients (mean dialytic age: 4.2 yrs.), FC and AC were analyzed in plasma (n=46 and n=31), resp.), in muscle biopsy specimen (n=lS and n=9, resp.) and in urine or dialysis fluid (n=lS and n=8, resp.) by using an optimized radiochemical-enzymatic method. P L A S MAa’ MUSCL@ IRIfQDIAL~Is FI.IJIDc) TC FC AC/FC TC FC AC/FC TC FC AC/FC HEALTHY CONTROLS mean 51.5 38.9 0.34 28.9 25.2 0.19 2451 1006 2.14 SEM 2.16 1.80 0.03 0.98 0.92 0.05 273 169 0.36 H&Patients 60.2* 32.4” mean 0.89*** 31.2 26.2 0.21 2435 797 2.24 SEM 2.93 1.88 0.05 2.71 2.14 0.04 366 106 0.42 al wl/l bl pi/g alkali-soluble protein c) wl,%.eek *p
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of carnitine on regeneration of the Z. Zadak, J. %mek, M. Hole&k, L. Sobotka University, Hradec Kralove, Czechoslovakia).
liver after partial hepatectomy in rats. (2nd Department of Intern. Med., Charles
The decrease in carnitine following severe liver damage and literary data suggesting that the liver tissue obtains energy primarily through FFA oxidation leads to the question how the administration of carnitine influences regeneration of the liver after partial hepatectomy (PH). Male rats of the Wistar strain were injected i.p. carnitinein dosesof 5 mg, 10 mg and 20 mg/kg body mass immediately and 6 hours after PH. Control rats were administered physiological saline. The course of liver regeneration was assessed on the basis of changes in specific DNA activity and of total DNA content in the liver 18 and 24 hours after PH. Eighteen hours after PH, i.e. during the onset of DNA synthesis, the values of specific DNA activity in rats following the administration of carnitine were significantly higher as compared with the control group (p< 0.01). Twenty-four hours after PH, this change induced an increase in total DNA in the liver of rats injected with carin the content of DNA depended nitine in comparison to the control group. The increase on the magnitude of the dose of carnitine administered (pd 0.05). Twenty-four hours after administration of carnitine, specific activity of DNA in the liver was significantly lower as compared with the controls (p< 0.05). The increase in specific act. of DNA in the liver 18 hours after PH, the decrease in the activity after 24 hours and the statistically significant increase in total DNA in the liver after 24 hours, as compared with the control group, suggest accelerated onset of liver regeneration after administration of carnitine.
IN HJXODIALYSIS(HD) PATIENTS?C.RGssle(l),K.P.Kohse(2) P.78 DISTURBEDCARNITINEMETABOLISM
W.Kapp(Z),H.-E.Franz(Z), A.GlGggler(l ),J.Bergstr&n(31 and P.Fiim (1) Inst. for Biol. Chem. and Nutr., Univ. of Hohenheim, Stuttgart, FRG; (2) Dept. of Nephrol., Univ. Hospital Ulm, FRG; (3) Dept. of Renal Medicine, Univ. Hospital Huddinge, Sweden.
An increase in the ratio of acylcarnitines (AC) to free camitine (FC) is a commonobservatron m HD-patients. The present study was designed to evaluate whether this abnormality is due to 1) extensive losses of FC through the dialysis fluid, 2) an incomplete oxidation of free fatty acids own to a total camitine (TC) deficiency in muscle or 3) a restricted mitochondrial transport of AC. In healthy controls and long-term HD-patients (mean dialytic age: 4.2 yrs.), FC and AC were analyzed in plasma (n=46 and n=31), resp.), in muscle biopsy specimen (n=lS and n=9, resp.) and in urine or dialysis fluid (n=lS and n=8, resp.) by using an optimized radiochemical-enzymatic method. P L A S MAa’ MUSCL@ IRIfQDIAL~Is FI.IJIDc) TC FC AC/FC TC FC AC/FC TC FC AC/FC HEALTHY CONTROLS mean 51.5 38.9 0.34 28.9 25.2 0.19 2451 1006 2.14 SEM 2.16 1.80 0.03 0.98 0.92 0.05 273 169 0.36 H&Patients 60.2* 32.4” mean 0.89*** 31.2 26.2 0.21 2435 797 2.24 SEM 2.93 1.88 0.05 2.71 2.14 0.04 366 106 0.42 al wl/l bl pi/g alkali-soluble protein c) wl,%.eek *p
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