- Email: [email protected]
Diversity, morphology, and phylogeny of the marine pulp-producing alga gelidiales (rhodophyta)
Abstracts / Journal of Biotechnology 136S (2008) S558–S576
VI4-O-024 Role of acetate and light attenuation in hydrogen production by chlamydomonas reinhardii B. Degrenne, G. Cogne, J. Pruvost, J. Legrand ∗ GEPEA, Université de Nantes, CNRS, UMR 6144, BP 406, 44602 SaintNazaire Cedex France The green fresh mircoalgae Chlamydomonas reinhardtii is known to produce hydrogen under anoxic conditions. Sulphur deprivation is commonly used to induce those conditions, by inducing a partial inhibition of PSII activity, reducing thus the oxygen release by photosynthesis. But, this protocol is also usually applied in photoheterotrophic conditions (in the presence of acetate). So, exacts effects of both sulphur deprivation and acetate are not clear. In this context, the growth of Chlamydomonas reinhardtii was characterized under three different conditions (autotrophic, photoheterotrophic and photoheterotrophic under sulphur deprived conditions) in a fully controlled photobioreactor. As described in literature, photoheterotrophic conditions under sulphur deprivation lead to anoxia and significant hydrogen production. But PSII inhibition by sulphur deprivation revealed also to be not necessary to reach anoxic conditions when cultivated in photoheterotrophic conditions. Acetate only was sufficient to obtain anoxic conditions involving next a low production of hydrogen. When acetate was totally consumed, oxygen release was observed and hydrogen production stopped. Comparison of all these results emphasizes the important role of acetate as well as light attenuation inside the culture to reach anoxic and hydrogen production conditions. The light fraction, characterizing the ratio between illuminated and dark zone in the reactor, was shown to be of great relevance on net oxygen production by the culture. If properly defined, anoxic conditions and hydrogen production in fully autotrophic conditions can be obtained, without PSII inhibition by sulphur deprivation.
S559
duction in May in Korea. Pterocladiella capillacea occurs world-wide and P. tenuis is distributed in the east Asian waters. In both species, rhizoidal filaments are produced from the cortical cells and become abundant in the medullar layers, but more abundant in P. tenuis than P. capillacea. In P. capillacea, the density of rhizoidal filaments tends to decrease in female thalli. Both cox1 and rbcL sequences from samples collected in Korea, France, and New Zealand consistently produced sister relationships between P. capillacea and P. tenuis. More than 15 cox1 haplotypes were found in P. capillacea, reflecting distributional patterns of the species. We present phylogenetic relationships of gelidoid algae and morphology of rhizoidal filaments under SEM and TEM. This work is by MarineBio21 and Pegasus International. References Lee, M.W., Han, S.O., Seo, Y.M., 2008. Red algae fibre/poly (butylene succinate) biocomposite: the effect of fibre content on their mechanical and thermal properties. Comp. Sci. Technol. 68, 1266–1272. Santelices, B., 2007. Testing the usefulness of attachment structures in the taxonomy of small sized gelidioids. Phycologia 46, 293–299.
doi:10.1016/j.jbiotec.2008.07.1316 VI4-Y-004 The optimization for cell cultivation of transgenic Laminaria japonica gametophytes in a bubble-column bioreactor Xiangyuan Deng 1,2 , Qun Zhang 1,2 , Peng Jiang 1 , Yu-Lin Cui 1,2 , Song Qin 1,∗ 1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
E-mail address: [email protected] (S. Qin). doi:10.1016/j.jbiotec.2008.07.1315 VI4-O-129 Diversity, morphology, and phylogeny of the marine pulpproducing alga gelidiales (rhodophyta) Sung Min Boo 1,∗ , Kyungmee Kim 1 , Il Ki Hwang 1 , Paul John. Geraldino 1 , Eun Chan Yang 1 , Yung Bum Seo 2 , Hwan Su Yoon 3 1
Department of Biology, Chungnam National University, Daejeon 305764, Republic of Korea 2 Department of Forest Science, Chungnam National University, Republic of Korea 3 Bigelow Laboratory for Ocean Sciences, USA E-mail address: [email protected] (S.M. Boo).
Recent algal technology exhibits a possibility of revolutionary change in paper industry, which has been absolutely dependant on wood. Marine pulp is produced from rhizoidal filaments in the cortical and medullar layer of the gelidioid red algae (Lee et al., 2008). We investigated diversity, morphology, and phylogeny of the Gelidiales and followed ontogeny of rhizoidal filaments under light microscopy, scanning and transmission electron microscopy. The Gelidiales consists of three families such Gelidiaceae, Gelidiellaceae, and Pterocladiaceae, containing approximately 176 species in the world. However, the species are still difficult to identify when vegetative or tetrasporangial stage (Santelices, 2007). Gelidium elegans, previously named as G. amansii, is the most common species in the gelidioids in the northeast Asia and is harvested for agar pro-
Human acidic fibroblast growth factor (haFGF) has strong mitogenic activity in vitro for various cells of mesodermal and neuroectodermal origin (Gospodarowicz et al., 1986) and plays a key role in regulating angiogenesis and neovascularization during development, wound healing and tumor growth (Fernig and Gallagher, 1994). It has been produced in Escherichia coli, yeast, and baculovirus-infected insect cells; however, there are few studies on expression of hafgf gene in algae. At present, a genetic transformation model for L. japonica has been developed by Qin et al. (2004) to express many foreign genes. In this study, gametophyte cells of L. japonica were bombarded with gold particles coated with plasmid DNA containing hafgf gene. Transformants were picked out via screening with phosphiothricin, and then cultured in a 300 ml bubble-column photobioreactor to optimize the growth conditions. The maximum final cell density (1695 mg DCW/L) was obtained over a 20 day cultivation period at a constant initial cell density of 127 mg DCW/L, aeration rate of 1.2 vvm, and inorganic nitrate and phosphate concentrations of 1.5 mM and 0.15 mM, respectively. The effect of different nitrogen sources (sodium nitrate, urea, ammonium sulfate and ammonium nitrate) on the growth of transgenic L. japonica gametophyte cells was carried out. The results showed that urea or sodium nitrate was the best nitrogen source, while ammonium ions suppressed the growth of gametophyte cells.
∗ Corresponding author. Tel.: +86 532 82898500; fax: +86 532 82898500.
VI4-O-024 Role of acetate and light attenuation in hydrogen production by chlamydomonas reinhardii B. Degrenne, G. Cogne, J. Pruvost, J. Legrand ∗ GEPEA, Université de Nantes, CNRS, UMR 6144, BP 406, 44602 SaintNazaire Cedex France The green fresh mircoalgae Chlamydomonas reinhardtii is known to produce hydrogen under anoxic conditions. Sulphur deprivation is commonly used to induce those conditions, by inducing a partial inhibition of PSII activity, reducing thus the oxygen release by photosynthesis. But, this protocol is also usually applied in photoheterotrophic conditions (in the presence of acetate). So, exacts effects of both sulphur deprivation and acetate are not clear. In this context, the growth of Chlamydomonas reinhardtii was characterized under three different conditions (autotrophic, photoheterotrophic and photoheterotrophic under sulphur deprived conditions) in a fully controlled photobioreactor. As described in literature, photoheterotrophic conditions under sulphur deprivation lead to anoxia and significant hydrogen production. But PSII inhibition by sulphur deprivation revealed also to be not necessary to reach anoxic conditions when cultivated in photoheterotrophic conditions. Acetate only was sufficient to obtain anoxic conditions involving next a low production of hydrogen. When acetate was totally consumed, oxygen release was observed and hydrogen production stopped. Comparison of all these results emphasizes the important role of acetate as well as light attenuation inside the culture to reach anoxic and hydrogen production conditions. The light fraction, characterizing the ratio between illuminated and dark zone in the reactor, was shown to be of great relevance on net oxygen production by the culture. If properly defined, anoxic conditions and hydrogen production in fully autotrophic conditions can be obtained, without PSII inhibition by sulphur deprivation.
S559
duction in May in Korea. Pterocladiella capillacea occurs world-wide and P. tenuis is distributed in the east Asian waters. In both species, rhizoidal filaments are produced from the cortical cells and become abundant in the medullar layers, but more abundant in P. tenuis than P. capillacea. In P. capillacea, the density of rhizoidal filaments tends to decrease in female thalli. Both cox1 and rbcL sequences from samples collected in Korea, France, and New Zealand consistently produced sister relationships between P. capillacea and P. tenuis. More than 15 cox1 haplotypes were found in P. capillacea, reflecting distributional patterns of the species. We present phylogenetic relationships of gelidoid algae and morphology of rhizoidal filaments under SEM and TEM. This work is by MarineBio21 and Pegasus International. References Lee, M.W., Han, S.O., Seo, Y.M., 2008. Red algae fibre/poly (butylene succinate) biocomposite: the effect of fibre content on their mechanical and thermal properties. Comp. Sci. Technol. 68, 1266–1272. Santelices, B., 2007. Testing the usefulness of attachment structures in the taxonomy of small sized gelidioids. Phycologia 46, 293–299.
doi:10.1016/j.jbiotec.2008.07.1316 VI4-Y-004 The optimization for cell cultivation of transgenic Laminaria japonica gametophytes in a bubble-column bioreactor Xiangyuan Deng 1,2 , Qun Zhang 1,2 , Peng Jiang 1 , Yu-Lin Cui 1,2 , Song Qin 1,∗ 1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
E-mail address: [email protected] (S. Qin). doi:10.1016/j.jbiotec.2008.07.1315 VI4-O-129 Diversity, morphology, and phylogeny of the marine pulpproducing alga gelidiales (rhodophyta) Sung Min Boo 1,∗ , Kyungmee Kim 1 , Il Ki Hwang 1 , Paul John. Geraldino 1 , Eun Chan Yang 1 , Yung Bum Seo 2 , Hwan Su Yoon 3 1
Department of Biology, Chungnam National University, Daejeon 305764, Republic of Korea 2 Department of Forest Science, Chungnam National University, Republic of Korea 3 Bigelow Laboratory for Ocean Sciences, USA E-mail address: [email protected] (S.M. Boo).
Recent algal technology exhibits a possibility of revolutionary change in paper industry, which has been absolutely dependant on wood. Marine pulp is produced from rhizoidal filaments in the cortical and medullar layer of the gelidioid red algae (Lee et al., 2008). We investigated diversity, morphology, and phylogeny of the Gelidiales and followed ontogeny of rhizoidal filaments under light microscopy, scanning and transmission electron microscopy. The Gelidiales consists of three families such Gelidiaceae, Gelidiellaceae, and Pterocladiaceae, containing approximately 176 species in the world. However, the species are still difficult to identify when vegetative or tetrasporangial stage (Santelices, 2007). Gelidium elegans, previously named as G. amansii, is the most common species in the gelidioids in the northeast Asia and is harvested for agar pro-
Human acidic fibroblast growth factor (haFGF) has strong mitogenic activity in vitro for various cells of mesodermal and neuroectodermal origin (Gospodarowicz et al., 1986) and plays a key role in regulating angiogenesis and neovascularization during development, wound healing and tumor growth (Fernig and Gallagher, 1994). It has been produced in Escherichia coli, yeast, and baculovirus-infected insect cells; however, there are few studies on expression of hafgf gene in algae. At present, a genetic transformation model for L. japonica has been developed by Qin et al. (2004) to express many foreign genes. In this study, gametophyte cells of L. japonica were bombarded with gold particles coated with plasmid DNA containing hafgf gene. Transformants were picked out via screening with phosphiothricin, and then cultured in a 300 ml bubble-column photobioreactor to optimize the growth conditions. The maximum final cell density (1695 mg DCW/L) was obtained over a 20 day cultivation period at a constant initial cell density of 127 mg DCW/L, aeration rate of 1.2 vvm, and inorganic nitrate and phosphate concentrations of 1.5 mM and 0.15 mM, respectively. The effect of different nitrogen sources (sodium nitrate, urea, ammonium sulfate and ammonium nitrate) on the growth of transgenic L. japonica gametophyte cells was carried out. The results showed that urea or sodium nitrate was the best nitrogen source, while ammonium ions suppressed the growth of gametophyte cells.
∗ Corresponding author. Tel.: +86 532 82898500; fax: +86 532 82898500.