- Email: [email protected]
DNA damage and cytogenetic alterations in formaldehyde exposed workers
S106
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
Extraction of hyaluronic acid from eggshell membranes Katarína
Vulganová 1 ,
Eva
Ürgeová 2
1
Institute of Physiotherapy, Balneology and Medical Rehabilitation, University of SS. Cyril and Methodius, SK 917 01 Trnava, Slovakia 2 Faculty of Natural Sciences, University of SS. Cyril and Methodius, SK 917 01 Trnava, Slovakia E-mail address: [email protected] (K. Vulganová). Hyaluronic acid is a natural polysaccharide occurring in human body, as an important part of the extracellular matrix of connective tissue. It is completely biocompatible therefore used for pharmaceutical purposes. It is a part of the nasal mucosa, periodontal synovial fluid, articular cartilage and skin. HA plays an important role in angiogenesis and tissue morphogenesis. It is also the marker signalling a myriad of disorders such as liver disease, cancer and rheumatoid lung disease. HA is usable for the treatment of osteoarthritis. It could be used in gynecology, dermatology, in rhinology where is successfully administered to minimise adverse side effects. In our paper, we present the isolation of the HA from the eggshell membranes by enzymatic hydrolysis using pepsin. We found specific conditions of hydrolysis: it progresses for 5 hours at 37◦ C, ratio 1 g of the sample to 30 ml buffer with pH = 3. The content of HA was tested spectrophotometrically at = 290 nm. http://dx.doi.org/10.1016/j.copbio.2013.05.324 DNA damage and cytogenetic alterations in formaldehyde exposed workers Solange Costa 1 , Carla Costa 1 , Patricia Coelho 1 , Susana Silva 1 , Beatriz Porto 2 , João Paulo Teixeira 1 1
National Institute of Health, Environmental Health Department, Porto, Portugal 2 Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal E-mail address: [email protected] (S. Costa). Genomic damage is probably the primary elemental cause of developmental and degenerative disease; it may result from environmental and/or occupational exposure, lifestyle factors and individual susceptibility. At least 200 000 people die every year from work-related cancers. A wide range of methods are currently used for the detection of early biological effects of genotoxic agents in occupational settings. Formaldehyde (FA) is a high volume production chemical with a wide range of industrial and medical uses. Listed, since 2004, by IARC as human carcinogen, FA status was recently revised by U.S. government who reclassified this compound as known to be a human carcinogen. To evaluate the potential genotoxic damage in workers exposed to FA a group of pathology and anatomy workers was tested for micronuclei frequency and DNA damage analysis by comet assay. Air sampling was performed in worker’s breathing zone for representative working periods and 8 hours-TWA was assessed. Genotoxic endpoints were significantly higher in the exposed subjects when compared with controls. Implementation of security and hygiene measures and good practices campaigns may be crucial to decrease risk. Acknowledgement: This work is supported by Fundac¸ão para a Ciência e a Tecnologia (FCT) under the grants SFRH/BD/46929/2008 and PTDC/SAU-ESA/102367/2008. http://dx.doi.org/10.1016/j.copbio.2013.05.325
The influence of the Melampyrum cristatum and Salviae officinalis tinctures on the Candida sp. in the oral cavity Munteanu Melania Florina, Onet Melinda, Gligor Ramona Medicine, “Vasile Goldis” Western University of Arad, Romania E-mail address: [email protected] (O. Melinda). Lack of equilibrium between the microorganism pathogenicity and the defense ability of the host combined with the debilitation of the immune system, leads to the aggravation of the Candida sp. infections. The antimycotic action of Melampyrum cristatum and Salviae officinalis tinctures on the Candida sp. The study included 45 patients, age 18–45 years, 25 females, 15 males and 10 children (age 8–14 years). The saliva was prelevated and subjected to the mycotic analysis for the yeast observation. In positive patients, the yeast was cultured on Mueller Hinton media, studying the effect the tinctures at different concentrations. For control there were used: Candida albicans (CA) ATCC 10231 and Candida krusei (CK) ATCC 6258. In the saliva, 14 female subjects have shown CA and 6 CK, while in males, 12 subjects have shown CA and 3 CK, 8 children were detected with CA and 2 with CK. The tinctures of M. cristatum and S. officinalis represent a good solution for designing new products. It was observed that S. officinalis tincture inhibited at a 1/4 dilution, having a 16 mm inhibition diameter and M. cristatum tincture inhibits at a 1/8 dilution with 18 mm inhibition diameter, proving an antimicrobial efficiency. http://dx.doi.org/10.1016/j.copbio.2013.05.326 Designing and production of a chimeric multi-epitope construct of CfaB, ST toxoid, CssA, CssB and LTB against enterotoxigenic E. coli Narges Zeinalzadeh 1 , Ali Hatef Salmanian 1 , Ghasem Ahangari 3 , Mehdi Sadeghi 2 , Jafar Amani 4 , Mahyat Jafari 1 1
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran 2 Department of Basic Science, NIGEB, Tehran, Iran 3 Department of Medical Biotechnology, NIGEB, Tehran, Iran 4 Applied Biotechnology Research Center, Baqiyatallah Medical Science University, Tehran, Iran E-mail address: [email protected] (N. Zeinalzadeh). Enterotoxigenic Escherichia coli (ETEC) strains are the most common cause of bacterial diarrhea in children in developing countries and travelers to these areas. Enterotoxins and colonization factors (CFs) are two key virulence factors in ETEC pathogenesis and the heterogeneity of the CFs is the bottleneck in reaching an effective vaccine. In this study a candidate subunit vaccine which is composed of CfaB, CssA and CssB, structural subunits of CFA/I and CS6 colonization factors, LTB, the binding subunit of LT and ST toxoid, was designed to provide broad spectrum of protection against ETEC. The different features of chimeric gene, its mRNA structure and chimeric protein properties were analyzed by using bioinformatics tools. The optimized chimeric gene was chemically synthesized and expressed successfully in prokaryotic host. The purified recombinant protein was used for assessment of bioinformatics data. http://dx.doi.org/10.1016/j.copbio.2013.05.327
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
Extraction of hyaluronic acid from eggshell membranes Katarína
Vulganová 1 ,
Eva
Ürgeová 2
1
Institute of Physiotherapy, Balneology and Medical Rehabilitation, University of SS. Cyril and Methodius, SK 917 01 Trnava, Slovakia 2 Faculty of Natural Sciences, University of SS. Cyril and Methodius, SK 917 01 Trnava, Slovakia E-mail address: [email protected] (K. Vulganová). Hyaluronic acid is a natural polysaccharide occurring in human body, as an important part of the extracellular matrix of connective tissue. It is completely biocompatible therefore used for pharmaceutical purposes. It is a part of the nasal mucosa, periodontal synovial fluid, articular cartilage and skin. HA plays an important role in angiogenesis and tissue morphogenesis. It is also the marker signalling a myriad of disorders such as liver disease, cancer and rheumatoid lung disease. HA is usable for the treatment of osteoarthritis. It could be used in gynecology, dermatology, in rhinology where is successfully administered to minimise adverse side effects. In our paper, we present the isolation of the HA from the eggshell membranes by enzymatic hydrolysis using pepsin. We found specific conditions of hydrolysis: it progresses for 5 hours at 37◦ C, ratio 1 g of the sample to 30 ml buffer with pH = 3. The content of HA was tested spectrophotometrically at = 290 nm. http://dx.doi.org/10.1016/j.copbio.2013.05.324 DNA damage and cytogenetic alterations in formaldehyde exposed workers Solange Costa 1 , Carla Costa 1 , Patricia Coelho 1 , Susana Silva 1 , Beatriz Porto 2 , João Paulo Teixeira 1 1
National Institute of Health, Environmental Health Department, Porto, Portugal 2 Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal E-mail address: [email protected] (S. Costa). Genomic damage is probably the primary elemental cause of developmental and degenerative disease; it may result from environmental and/or occupational exposure, lifestyle factors and individual susceptibility. At least 200 000 people die every year from work-related cancers. A wide range of methods are currently used for the detection of early biological effects of genotoxic agents in occupational settings. Formaldehyde (FA) is a high volume production chemical with a wide range of industrial and medical uses. Listed, since 2004, by IARC as human carcinogen, FA status was recently revised by U.S. government who reclassified this compound as known to be a human carcinogen. To evaluate the potential genotoxic damage in workers exposed to FA a group of pathology and anatomy workers was tested for micronuclei frequency and DNA damage analysis by comet assay. Air sampling was performed in worker’s breathing zone for representative working periods and 8 hours-TWA was assessed. Genotoxic endpoints were significantly higher in the exposed subjects when compared with controls. Implementation of security and hygiene measures and good practices campaigns may be crucial to decrease risk. Acknowledgement: This work is supported by Fundac¸ão para a Ciência e a Tecnologia (FCT) under the grants SFRH/BD/46929/2008 and PTDC/SAU-ESA/102367/2008. http://dx.doi.org/10.1016/j.copbio.2013.05.325
The influence of the Melampyrum cristatum and Salviae officinalis tinctures on the Candida sp. in the oral cavity Munteanu Melania Florina, Onet Melinda, Gligor Ramona Medicine, “Vasile Goldis” Western University of Arad, Romania E-mail address: [email protected] (O. Melinda). Lack of equilibrium between the microorganism pathogenicity and the defense ability of the host combined with the debilitation of the immune system, leads to the aggravation of the Candida sp. infections. The antimycotic action of Melampyrum cristatum and Salviae officinalis tinctures on the Candida sp. The study included 45 patients, age 18–45 years, 25 females, 15 males and 10 children (age 8–14 years). The saliva was prelevated and subjected to the mycotic analysis for the yeast observation. In positive patients, the yeast was cultured on Mueller Hinton media, studying the effect the tinctures at different concentrations. For control there were used: Candida albicans (CA) ATCC 10231 and Candida krusei (CK) ATCC 6258. In the saliva, 14 female subjects have shown CA and 6 CK, while in males, 12 subjects have shown CA and 3 CK, 8 children were detected with CA and 2 with CK. The tinctures of M. cristatum and S. officinalis represent a good solution for designing new products. It was observed that S. officinalis tincture inhibited at a 1/4 dilution, having a 16 mm inhibition diameter and M. cristatum tincture inhibits at a 1/8 dilution with 18 mm inhibition diameter, proving an antimicrobial efficiency. http://dx.doi.org/10.1016/j.copbio.2013.05.326 Designing and production of a chimeric multi-epitope construct of CfaB, ST toxoid, CssA, CssB and LTB against enterotoxigenic E. coli Narges Zeinalzadeh 1 , Ali Hatef Salmanian 1 , Ghasem Ahangari 3 , Mehdi Sadeghi 2 , Jafar Amani 4 , Mahyat Jafari 1 1
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran 2 Department of Basic Science, NIGEB, Tehran, Iran 3 Department of Medical Biotechnology, NIGEB, Tehran, Iran 4 Applied Biotechnology Research Center, Baqiyatallah Medical Science University, Tehran, Iran E-mail address: [email protected] (N. Zeinalzadeh). Enterotoxigenic Escherichia coli (ETEC) strains are the most common cause of bacterial diarrhea in children in developing countries and travelers to these areas. Enterotoxins and colonization factors (CFs) are two key virulence factors in ETEC pathogenesis and the heterogeneity of the CFs is the bottleneck in reaching an effective vaccine. In this study a candidate subunit vaccine which is composed of CfaB, CssA and CssB, structural subunits of CFA/I and CS6 colonization factors, LTB, the binding subunit of LT and ST toxoid, was designed to provide broad spectrum of protection against ETEC. The different features of chimeric gene, its mRNA structure and chimeric protein properties were analyzed by using bioinformatics tools. The optimized chimeric gene was chemically synthesized and expressed successfully in prokaryotic host. The purified recombinant protein was used for assessment of bioinformatics data. http://dx.doi.org/10.1016/j.copbio.2013.05.327