- Email: [email protected]
Do diet and exercise influence in an athletic population?: New clues of the entero-insular axis
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Abstracts / Atherosclerosis 252 (2016) e1ee196
dietary guidelines and were randomized to the following treatment: 1hazelnuts with skin; 2- hazelnuts without skin; 3- control group (no hazelnuts). Before and after intervention, blood samples were collected for the evaluation of serum lipid levels, fatty acid composition of erythrocytes membrane and levels of endogenous and oxidatively-induced DNA damage in peripheral blood mononuclear cells. Results: The regular intake of hazelnuts, with and without skin, significantly (p<0.05) reduced the levels of serum LDL-cholesterol and endogenous DNA damage, while increased the ratio of MUFA/SFA and MUFA in erythrocytes membrane. Furthermore, the consumption of hazelnut with skin significantly decreased the levels of oxidatively-induced DNA damage. No effect was observed in the control group. Conclusions: Our findings suggest a beneficial effect of hazelnut-enriched diet in the management of dyslipidemia and oxidative stress in children.
EAS16-0655, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. PLASMA PLANT STEROLS AND PLANT STEROL INTAKE VARY ACROSS PLANT-BASED DIETARY PATTERNS AMONG ADULTS FROM NORTH AMERICA D. Luetjohann 1, K. Jaceldo-Siegl 2, 3, R. Sirirat 3, E. Haddad 2, 3. 1 University of Bonn, Institute for Clinical Chemistry and Clinical Pharmacology, Bonn, Germany; 2 Loma Linda University, Center for Nutrition- Healthy Lifestyles and Disease Prevention- School of Public Health, Loma Linda- CA, USA; 3 Loma Linda University, Adventist Health Studies, Loma Linda- CA, USA Objectives: Plant sterols have cholesterol-lowering properties, which may have benefits on mortality and cardiovascular disease. Plant-based dietary patterns also have similar attributes. Variations in plasma plant sterols and plant sterol intake in plant-based diets are examined. Methods: We conducted cross-sectional analysis on data from the Adventist Health Study-2 Calibration Sub-study (n ¼ 861). Participants (66% females, average age 61 yrs.) completed a food frequency questionnaire, provided six 24-hour dietary recalls, demographic and biometric data, and collection of fasting blood samples. Plasma cholesterol and noncholesterol sterols were analyzed by gas-liquid chromatography-flame ionization detection. We used analysis of variance controlling for age, race, gender, and BMI to determine differences across plant-based dietary patterns and applied Tukey-Kramer test to adjust for multiple comparisons. Results: In univariate analyses, mean (SD) intake of total plant sterols was 309 (49) mg/day, and cholesterol 83 (34) mg/day; mean plasma campesterol, b-sitosterol, and cholesterol levels were 4.2 (2.3) mg/mL, 3.3 (1.6) mg/ mL, and 192 (39) mg/dL, respectively. Cholesterol intake was lowest among vegans (39.0 mg/d) and highest among non-vegetarians (96.1 mg/d; P < 0.0001). Plasma cholesterol did not differ by diet. Plant sterol intake differed significantly across diets, and generally was lowest among nonvegetarians, and highest among vegans. In multivariable analyses, plasma b-sitosterol was significantly higher in vegans (4.45 mg/mL) than in nonvegetarians (3.98 mg/mL; P ¼ 0.04). Conclusions: We provide evidence that intake of plant sterols and cholesterol and plasma b-sitosterol are associated with plant-based dietary patterns independent of age, race, gender and BMI.
EAS16-0999, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. LOW-FAT DIETARY TREATMENT ENRICHED WITH SOY PROTEIN IN CHILDREN AFFECTED WITH HETEROZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA: RESULTS FROM A RANDOMIZEDCONTROLLED PILOT STUDY O. Helk, K. Widhalm. Austrian Academic Institute for Clinical Nutrition, Austrian Academic Institute for Clinical Nutrition, Vienna, Austria Objectives: Heterozygous familial hypercholesterolemia (heFH) is an inheritable, autosomal dominant disorder leading to pathologically increased levels of low-density-lipoprotein cholesterol (LDL-C). The aim of the present pilot study is to examine the effectiveness and safety of a diet enriched with soy-protein for LDL-C reduction in children affected with heFH.
Methods: Patients were randomly allocated to either a treatment group with a soy-enriched, fat modified diet (soy group) or a fat modified diet alone (FM group). Blood was sampled at baseline, week 7 and week 13. All subjects were asked to provide weekly urine samples, in which the isoflavones daidzein and genistein and their respective metabolites were measured, creatinine adjusted, summarized and then correlated with LDLC decrease from baseline (Dai+Met, Gen+Met). Results: 25 patients completed the study. A statistically significant interaction between group and time on cholesterol concentration, F(2,42) ¼ 7.118, p ¼ 0.002, partial h2 ¼ 0.253 was detected. Mean LDL-C at baseline, week 7 and week 13 was 175.64 (±14.20) , 153.64 (±17.48) and 155.09 (±13.435) for the soy group and 168.08 (±19.47), 171.58 (±22.96) and 171.08 (±28.60) for the FM group.Significant correlations with LDLdecrease in percent from baseline were found in week 7 for both Dai+Met (p¼ 0.085, r ¼ 0.330) and Gen+Met (p¼.190, r ¼ 0.284). No significant correlations were found in week 13. Conclusions: We provide evidence from a randomized controlled trial that a diet enriched with soy-protein may be a viable option for dietary treatment of paediatric patients affected with heFH.
EAS16-0369, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. ANGIOGRAPHIC AND ANTIOXIDANT CHANGES AFTER SUPPLEMENTATION WITH HERACLEUM PERSICUM EXTRACT: RESULTS OF A RANDOMIZED CONTROLLED TRIAL A. Sahebkar 1, Y. Panahi 2, H. Taghipour 3. 1 Mashhad University of Medical Sciences, Biotechnology Research Center, Mashhad, Iran; 2 Baqiyatallah University of Medical Sciences, Chemical Injuries Research Center, Tehran, Iran; 3 Baqiyatallah University of Medical Sciences- Tehran- Iran, Department of Cardiology and Cardiothoracic Surgery, Tehran, Iran Objectives: Heracleum persicum is a common dietary spice with several pharmacological effects relevant to cardiovascular health. This study explored the effects of supplementation with H. persicum fruit extract on the angiographic findings, lipid and antioxidant indices in patients with minimal coronary artery disease (CAD). Methods: Subjects with < 50% stenosis in any of their coronary arteries in angiography were recruited and randomly assigned to H. persicum fruit extract (n ¼ 15; 300 mg/day) or placebo (n ¼ 12) for a period of 6 months. At the end of trial, participants underwent a second coronary angiography in order to evaluate the progression of their disease. Blood samples were taken at baseline and at the end of trial to evaluate the changes in lipids and antioxidant indices including malondialdehyde (MDA), reduced glutathione (GSH) total antioxidant capacity (TAC), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT). Results: Angiographic findings did not reveal any improvement in the number of stenosed vessels nor in the chest pain severity after consumption of H. persicum extract versus placebo (p > 0.05). However, H. persicum supplementation reduced serum MDA (p¼0.001), and elevated serum GSH (p¼0.001), TAC (p¼0.001), GPx (p¼0.001) and CAT (p¼0.001), but had no effect on serum SOD (p>0.05). Conclusions: The present results suggest the antioxidant efficacy of H. persicum fruit extract but do not support any significant benefit of H. persicum extract supplementation on the angiographic findings of in patients with minimal CAD.
EAS16-0454, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. DO DIET AND EXERCISE INFLUENCE IN AN ATHLETIC POPULATION?: NEW CLUES OF THE ENTERO-INSULAR AXIS C. Rodriguez-Leal 1, V. Aragon 1, I. Tinoco-Racero 1, E. Segura 1, B. ruizestevez 1, E. Sanchez-Orellana 1, C. Perez 2, M. Monsalvo Hernando 1, N. A. Perez 1, I. Garcia-Campanario 2, A. Mangas 2, R. Caro 1, 2 1 diz, Toro . Universitary Hospital Puerta del Mar, Internal Medicine, Ca diz, Medicine Department, CADIZ, Spain; 2 Medicine School. University of Ca Spain
Abstracts / Atherosclerosis 252 (2016) e1ee196
Objectives: Physical exercise and appetite regulation can lead to improved competitive performance of athletes. Mediators of the entero-insular axis generate neurohumoral signals that influence on the appetite regulation and energy homeostasis. We aim to determine the diet influence and prolonged exercise on intestinal peptide, ghrelin, resistin, leptin, and incretins (GLP-1 and GIP) in an athlete population. Methods: This is a prospective interventional study. 32 healthy semiprofessional rugby players, aged 13-39 years were included. Plasma levels of intestinal peptide, ghrelin, resistin, leptin, and incretins were calculated. In addition, anthropometric measurements and blood samples were taken at time 0 and after six months of study. Athletes were randomized to a protein diet (PD) or Mediterranean diet (MD). Results: In the PD group, GLP-1 and GIP plasmatic levels showed a significant decrease (p <0.03; p <0.01 respectively). GLP-1 and ghrelin plasmatic concentration demonstrated a significant decrease (p <0.03 respectively) in those who increased muscle mass (MM). Finally, the athletes related to the PD who showed augmented total weight and MM presented significantly decreased GLP-1 concentration (p <0.03 and p<0.002, respectively). Conclusions: Athletes could avoid hypoglycemia using the PD that shows to decrease GLP-1 plasmatic concentration In certain sport modalities, PD could enhance the athletic performance, in certain sports modalities, promoting the MM and total weight gain.
EAS16-0489, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. EVALUATION OF ACTUAL CONSUMPTION OF PULSE REDUCING DRUGS IN PATIENTS AFTER ACUTE CORONARY SYNDROME T. Chudinovskikh 1, E. Tarlovskaya 2. 1 Kirov State Medical Academy, Department of Internal Medicine, Kirov, Russia; 2 Nizhny Novgorod State Medical Academy, Department of Internal Medicine, Nizhny Novgorod, Russia Objectives: To determine the “load” of pulse reducing drugs (PRD) on patients after an acute coronary syndrome with preserved ejection fraction to achieve a target heart rate (HR). Methods: 60 patients admitted to rehabilitation. Of these, 36 men and 24 women in the age of 35 to 75 years. The average age was 60,28 ± 8,03 years. Patients of group A received the original bisoprolol (Concor, Merck KGaA, Germany), patients in group B - generic (Niperten, Krka-Rus, Russia). Target HR was considered as 60 b.p.m or less. Ivabradine (Coraxan, Servier, France) was administrated to main therapy in the cases of target HR absence and there was not opportunity to increase the dose of betablockers (BAB). To evaluate the actual consumption of pulse slows drugs we used ATC/DDD-methodology. Results: To determine the actual drug consumption, amount of adjusting doses PRD was calculated in each group. Defined daily dose (NDDD) is calculated as the ratio of the drug number (Q-quantity) to the DDD (Defined Daily Dose). So the NDDD sum of the PRD is 33.5 mg per 1 person for 6 therapy weeks in group A, 44.2 mg in group B. In order to reduce the heart rate by 10 beats / min in group A necessary amount 28.15 mg NDDD pulse slows preparations for 6 weeks in group B - 48.0 mg. Conclusions: Analysis of the results showed that the DDD amount of PRD in the group of the original bisoprolol was significantly lower in comparison to generic drugs for achievement to target HR.
EAS16-1002, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. EFFECTS OF RAMADAN FASTING ON CLINICAL AND BIOCHEMICAL PARAMETERS FOR TYPE 2 DIABETICS K. Bouzid 1, Y. Molahedh 2, W. Hmaidi 2, N. Mchirgui 3, A. Bartkiz 2, E. Talbi 2, N. Ben Abdallah 3, A. Bahlous 2, J. Abdelmoula 2. 1 Hopital Charles Nicolle, Biochemistry-Laboratory of Engineering of Proteins and Bioactive Molecules INSAT: LR 11EES24:LIP-MB, Tunis, Tunisia; 2 Hopital Charles Nicolle, Biochemistry, Tunis, Tunisia; 3 Hopital Charles Nicolle, Endocrinology, Tunis, Tunisia
e93
Objectives: To determine if Ramadan fasting is safe in patients with type 2 diabetes mellitus (T2D), based upon a determination of the effect of fasting on clinical and biochemical parameters. Methods: We conducted a prospective study including 61 type 2 diabetic patients treated with diet or oral antidiabetics. Clinical and biochemical parameters were evaluated three weeks before Ramadan and one week after the end of Ramadan. Results: The sex-ratio of the patients was 0.79 and the mean age was 55 ± 8 years [34-69]. The average duration of diabetes was 4±2 years [1-15]. Ramadan fasting had no major effect on body mass index, diastolic blood pressure but systolic blood pressure had decreased significantly (p<0.001). Fasting glucose and insulin levels decreased as well as HOMA-IR index (p<0.001). There were fluctuations in some lipid parameters; the total cholesterol, LDL-cholesterol, HDL-cholesterol, and apolipoprotein A have decreased significantly (p<0.01). Triglycerides and apolipoprotein B didn’t change. A statistically significant increase was observed for folates and B12 vitamin (p<0.001). The C Reactive Protein (CRP) decreased significantly but there are no changes in homocysteine and protids levels. Conclusions: Our study found that Ramadan fasting is beneficial for type 2 diabetics but education is necessary to avoid diabetic metabolic complications.
EAS16-1036, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. COMPARISON OF EFFECTS OF DIABETIC ENTERAL NUTRITION AND STANDARD ENTERAL NUTRITION ON POSTPRANDIAL LEVEL OF GLYCEMIA, FREE FATTY ACIDS AND GLUCAGON-LIKE PEPTIDE-1. J. Visek 1, A. Ticha 2, L. Sobotka 1, V. Blaha 1. 1 Charles University- Medical Faculty and Faculty Hospital, Metabolic department, Hradec Kralove, Czech Republic; 2 Charles University- Medical Faculty and Faculty Hospital, Department of Research and Development, Hradec Kralove, Czech Republic Objectives: Use of standard enteral nutrition (EN) can cause hyperglycemia and associated complications. It may be recommended to use EN specially developed to minimize glycaemic response. On the other hand, these formulas often contain small amount of proteins, which could be a disadvantage. Methods: Eight type 2 diabetes mellitus patients treated with diet were involved. Three types of EN were compared: Nutrison MF (standard EN), Diben drink (regular hypocaloric diabetic nutrition) and Glucerna Select (isocaloric diabetic nutrition). We measured levels of blood glucose, free fatty acids (FFA) and Glucagon-like peptide-1 (GLP-1) at baseline and 15, 30, 45, 60, 90 and 120 minutes after ingestion of solution containing 25g of carbohydrates. Upward parts of the area under the curve (AUC) were compared. Measurements were repeated 3 times for each solution. Results: Statistical analysis was performed by One Way RM ANOVA. The glycemic index (GI) as a marker od postprandial glycemia of Nutrison MF was 62,5± 20,2, Diben drink 48,2±7,6 and Glucerna Select 28.1±7.5. Results of GI after Nutrison MF were statistically different from Glucerna Select (P <0.001). The plasma FFA after ingestion of Nutrison MF, Diben drink and Glucerna Select did not differ up to 60 minute. After that the plasma FFA remained significantly increased with Glucerna Select (P<0.001). The AUC of GLP-1 after ingestion of different types of EN were not statistically different. Conclusions: Our results indicate that diabetes-specific formulas of enteral nutrition could be beneficial for diabetic patients. This work was supported by Prvouk P37/12, IGA MH CR NT/12287-5
EAS16-0539, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. REGULATION OF HYPOTHALAMIC GENE EXPRESSION BY DNA METHYLATION IS INFLUENCED BY PARENTAL DIET DURING PREGNANCY AND LINKED TO OFFSPRING'S RISK OF OBESITY I. Khurana 1, A. Kaspi 1, M. Ziemann 1, T. Block 1, T. Connor 2, B. Spolding 2, A. Cooper 2, P. Zimmet 1, A. El-Osta 1, 3, 4, K. Walder 2. 1 Baker IDI Heart and Diabetes Institute, Human Epigenetics, Melbourne, Australia; 2 Faculty of Health- Medicine- Nursing and Behavioral Sciences- Deakin University,
Abstracts / Atherosclerosis 252 (2016) e1ee196
dietary guidelines and were randomized to the following treatment: 1hazelnuts with skin; 2- hazelnuts without skin; 3- control group (no hazelnuts). Before and after intervention, blood samples were collected for the evaluation of serum lipid levels, fatty acid composition of erythrocytes membrane and levels of endogenous and oxidatively-induced DNA damage in peripheral blood mononuclear cells. Results: The regular intake of hazelnuts, with and without skin, significantly (p<0.05) reduced the levels of serum LDL-cholesterol and endogenous DNA damage, while increased the ratio of MUFA/SFA and MUFA in erythrocytes membrane. Furthermore, the consumption of hazelnut with skin significantly decreased the levels of oxidatively-induced DNA damage. No effect was observed in the control group. Conclusions: Our findings suggest a beneficial effect of hazelnut-enriched diet in the management of dyslipidemia and oxidative stress in children.
EAS16-0655, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. PLASMA PLANT STEROLS AND PLANT STEROL INTAKE VARY ACROSS PLANT-BASED DIETARY PATTERNS AMONG ADULTS FROM NORTH AMERICA D. Luetjohann 1, K. Jaceldo-Siegl 2, 3, R. Sirirat 3, E. Haddad 2, 3. 1 University of Bonn, Institute for Clinical Chemistry and Clinical Pharmacology, Bonn, Germany; 2 Loma Linda University, Center for Nutrition- Healthy Lifestyles and Disease Prevention- School of Public Health, Loma Linda- CA, USA; 3 Loma Linda University, Adventist Health Studies, Loma Linda- CA, USA Objectives: Plant sterols have cholesterol-lowering properties, which may have benefits on mortality and cardiovascular disease. Plant-based dietary patterns also have similar attributes. Variations in plasma plant sterols and plant sterol intake in plant-based diets are examined. Methods: We conducted cross-sectional analysis on data from the Adventist Health Study-2 Calibration Sub-study (n ¼ 861). Participants (66% females, average age 61 yrs.) completed a food frequency questionnaire, provided six 24-hour dietary recalls, demographic and biometric data, and collection of fasting blood samples. Plasma cholesterol and noncholesterol sterols were analyzed by gas-liquid chromatography-flame ionization detection. We used analysis of variance controlling for age, race, gender, and BMI to determine differences across plant-based dietary patterns and applied Tukey-Kramer test to adjust for multiple comparisons. Results: In univariate analyses, mean (SD) intake of total plant sterols was 309 (49) mg/day, and cholesterol 83 (34) mg/day; mean plasma campesterol, b-sitosterol, and cholesterol levels were 4.2 (2.3) mg/mL, 3.3 (1.6) mg/ mL, and 192 (39) mg/dL, respectively. Cholesterol intake was lowest among vegans (39.0 mg/d) and highest among non-vegetarians (96.1 mg/d; P < 0.0001). Plasma cholesterol did not differ by diet. Plant sterol intake differed significantly across diets, and generally was lowest among nonvegetarians, and highest among vegans. In multivariable analyses, plasma b-sitosterol was significantly higher in vegans (4.45 mg/mL) than in nonvegetarians (3.98 mg/mL; P ¼ 0.04). Conclusions: We provide evidence that intake of plant sterols and cholesterol and plasma b-sitosterol are associated with plant-based dietary patterns independent of age, race, gender and BMI.
EAS16-0999, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. LOW-FAT DIETARY TREATMENT ENRICHED WITH SOY PROTEIN IN CHILDREN AFFECTED WITH HETEROZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA: RESULTS FROM A RANDOMIZEDCONTROLLED PILOT STUDY O. Helk, K. Widhalm. Austrian Academic Institute for Clinical Nutrition, Austrian Academic Institute for Clinical Nutrition, Vienna, Austria Objectives: Heterozygous familial hypercholesterolemia (heFH) is an inheritable, autosomal dominant disorder leading to pathologically increased levels of low-density-lipoprotein cholesterol (LDL-C). The aim of the present pilot study is to examine the effectiveness and safety of a diet enriched with soy-protein for LDL-C reduction in children affected with heFH.
Methods: Patients were randomly allocated to either a treatment group with a soy-enriched, fat modified diet (soy group) or a fat modified diet alone (FM group). Blood was sampled at baseline, week 7 and week 13. All subjects were asked to provide weekly urine samples, in which the isoflavones daidzein and genistein and their respective metabolites were measured, creatinine adjusted, summarized and then correlated with LDLC decrease from baseline (Dai+Met, Gen+Met). Results: 25 patients completed the study. A statistically significant interaction between group and time on cholesterol concentration, F(2,42) ¼ 7.118, p ¼ 0.002, partial h2 ¼ 0.253 was detected. Mean LDL-C at baseline, week 7 and week 13 was 175.64 (±14.20) , 153.64 (±17.48) and 155.09 (±13.435) for the soy group and 168.08 (±19.47), 171.58 (±22.96) and 171.08 (±28.60) for the FM group.Significant correlations with LDLdecrease in percent from baseline were found in week 7 for both Dai+Met (p¼ 0.085, r ¼ 0.330) and Gen+Met (p¼.190, r ¼ 0.284). No significant correlations were found in week 13. Conclusions: We provide evidence from a randomized controlled trial that a diet enriched with soy-protein may be a viable option for dietary treatment of paediatric patients affected with heFH.
EAS16-0369, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. ANGIOGRAPHIC AND ANTIOXIDANT CHANGES AFTER SUPPLEMENTATION WITH HERACLEUM PERSICUM EXTRACT: RESULTS OF A RANDOMIZED CONTROLLED TRIAL A. Sahebkar 1, Y. Panahi 2, H. Taghipour 3. 1 Mashhad University of Medical Sciences, Biotechnology Research Center, Mashhad, Iran; 2 Baqiyatallah University of Medical Sciences, Chemical Injuries Research Center, Tehran, Iran; 3 Baqiyatallah University of Medical Sciences- Tehran- Iran, Department of Cardiology and Cardiothoracic Surgery, Tehran, Iran Objectives: Heracleum persicum is a common dietary spice with several pharmacological effects relevant to cardiovascular health. This study explored the effects of supplementation with H. persicum fruit extract on the angiographic findings, lipid and antioxidant indices in patients with minimal coronary artery disease (CAD). Methods: Subjects with < 50% stenosis in any of their coronary arteries in angiography were recruited and randomly assigned to H. persicum fruit extract (n ¼ 15; 300 mg/day) or placebo (n ¼ 12) for a period of 6 months. At the end of trial, participants underwent a second coronary angiography in order to evaluate the progression of their disease. Blood samples were taken at baseline and at the end of trial to evaluate the changes in lipids and antioxidant indices including malondialdehyde (MDA), reduced glutathione (GSH) total antioxidant capacity (TAC), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT). Results: Angiographic findings did not reveal any improvement in the number of stenosed vessels nor in the chest pain severity after consumption of H. persicum extract versus placebo (p > 0.05). However, H. persicum supplementation reduced serum MDA (p¼0.001), and elevated serum GSH (p¼0.001), TAC (p¼0.001), GPx (p¼0.001) and CAT (p¼0.001), but had no effect on serum SOD (p>0.05). Conclusions: The present results suggest the antioxidant efficacy of H. persicum fruit extract but do not support any significant benefit of H. persicum extract supplementation on the angiographic findings of in patients with minimal CAD.
EAS16-0454, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. DO DIET AND EXERCISE INFLUENCE IN AN ATHLETIC POPULATION?: NEW CLUES OF THE ENTERO-INSULAR AXIS C. Rodriguez-Leal 1, V. Aragon 1, I. Tinoco-Racero 1, E. Segura 1, B. ruizestevez 1, E. Sanchez-Orellana 1, C. Perez 2, M. Monsalvo Hernando 1, N. A. Perez 1, I. Garcia-Campanario 2, A. Mangas 2, R. Caro 1, 2 1 diz, Toro . Universitary Hospital Puerta del Mar, Internal Medicine, Ca diz, Medicine Department, CADIZ, Spain; 2 Medicine School. University of Ca Spain
Abstracts / Atherosclerosis 252 (2016) e1ee196
Objectives: Physical exercise and appetite regulation can lead to improved competitive performance of athletes. Mediators of the entero-insular axis generate neurohumoral signals that influence on the appetite regulation and energy homeostasis. We aim to determine the diet influence and prolonged exercise on intestinal peptide, ghrelin, resistin, leptin, and incretins (GLP-1 and GIP) in an athlete population. Methods: This is a prospective interventional study. 32 healthy semiprofessional rugby players, aged 13-39 years were included. Plasma levels of intestinal peptide, ghrelin, resistin, leptin, and incretins were calculated. In addition, anthropometric measurements and blood samples were taken at time 0 and after six months of study. Athletes were randomized to a protein diet (PD) or Mediterranean diet (MD). Results: In the PD group, GLP-1 and GIP plasmatic levels showed a significant decrease (p <0.03; p <0.01 respectively). GLP-1 and ghrelin plasmatic concentration demonstrated a significant decrease (p <0.03 respectively) in those who increased muscle mass (MM). Finally, the athletes related to the PD who showed augmented total weight and MM presented significantly decreased GLP-1 concentration (p <0.03 and p<0.002, respectively). Conclusions: Athletes could avoid hypoglycemia using the PD that shows to decrease GLP-1 plasmatic concentration In certain sport modalities, PD could enhance the athletic performance, in certain sports modalities, promoting the MM and total weight gain.
EAS16-0489, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. EVALUATION OF ACTUAL CONSUMPTION OF PULSE REDUCING DRUGS IN PATIENTS AFTER ACUTE CORONARY SYNDROME T. Chudinovskikh 1, E. Tarlovskaya 2. 1 Kirov State Medical Academy, Department of Internal Medicine, Kirov, Russia; 2 Nizhny Novgorod State Medical Academy, Department of Internal Medicine, Nizhny Novgorod, Russia Objectives: To determine the “load” of pulse reducing drugs (PRD) on patients after an acute coronary syndrome with preserved ejection fraction to achieve a target heart rate (HR). Methods: 60 patients admitted to rehabilitation. Of these, 36 men and 24 women in the age of 35 to 75 years. The average age was 60,28 ± 8,03 years. Patients of group A received the original bisoprolol (Concor, Merck KGaA, Germany), patients in group B - generic (Niperten, Krka-Rus, Russia). Target HR was considered as 60 b.p.m or less. Ivabradine (Coraxan, Servier, France) was administrated to main therapy in the cases of target HR absence and there was not opportunity to increase the dose of betablockers (BAB). To evaluate the actual consumption of pulse slows drugs we used ATC/DDD-methodology. Results: To determine the actual drug consumption, amount of adjusting doses PRD was calculated in each group. Defined daily dose (NDDD) is calculated as the ratio of the drug number (Q-quantity) to the DDD (Defined Daily Dose). So the NDDD sum of the PRD is 33.5 mg per 1 person for 6 therapy weeks in group A, 44.2 mg in group B. In order to reduce the heart rate by 10 beats / min in group A necessary amount 28.15 mg NDDD pulse slows preparations for 6 weeks in group B - 48.0 mg. Conclusions: Analysis of the results showed that the DDD amount of PRD in the group of the original bisoprolol was significantly lower in comparison to generic drugs for achievement to target HR.
EAS16-1002, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. EFFECTS OF RAMADAN FASTING ON CLINICAL AND BIOCHEMICAL PARAMETERS FOR TYPE 2 DIABETICS K. Bouzid 1, Y. Molahedh 2, W. Hmaidi 2, N. Mchirgui 3, A. Bartkiz 2, E. Talbi 2, N. Ben Abdallah 3, A. Bahlous 2, J. Abdelmoula 2. 1 Hopital Charles Nicolle, Biochemistry-Laboratory of Engineering of Proteins and Bioactive Molecules INSAT: LR 11EES24:LIP-MB, Tunis, Tunisia; 2 Hopital Charles Nicolle, Biochemistry, Tunis, Tunisia; 3 Hopital Charles Nicolle, Endocrinology, Tunis, Tunisia
e93
Objectives: To determine if Ramadan fasting is safe in patients with type 2 diabetes mellitus (T2D), based upon a determination of the effect of fasting on clinical and biochemical parameters. Methods: We conducted a prospective study including 61 type 2 diabetic patients treated with diet or oral antidiabetics. Clinical and biochemical parameters were evaluated three weeks before Ramadan and one week after the end of Ramadan. Results: The sex-ratio of the patients was 0.79 and the mean age was 55 ± 8 years [34-69]. The average duration of diabetes was 4±2 years [1-15]. Ramadan fasting had no major effect on body mass index, diastolic blood pressure but systolic blood pressure had decreased significantly (p<0.001). Fasting glucose and insulin levels decreased as well as HOMA-IR index (p<0.001). There were fluctuations in some lipid parameters; the total cholesterol, LDL-cholesterol, HDL-cholesterol, and apolipoprotein A have decreased significantly (p<0.01). Triglycerides and apolipoprotein B didn’t change. A statistically significant increase was observed for folates and B12 vitamin (p<0.001). The C Reactive Protein (CRP) decreased significantly but there are no changes in homocysteine and protids levels. Conclusions: Our study found that Ramadan fasting is beneficial for type 2 diabetics but education is necessary to avoid diabetic metabolic complications.
EAS16-1036, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. COMPARISON OF EFFECTS OF DIABETIC ENTERAL NUTRITION AND STANDARD ENTERAL NUTRITION ON POSTPRANDIAL LEVEL OF GLYCEMIA, FREE FATTY ACIDS AND GLUCAGON-LIKE PEPTIDE-1. J. Visek 1, A. Ticha 2, L. Sobotka 1, V. Blaha 1. 1 Charles University- Medical Faculty and Faculty Hospital, Metabolic department, Hradec Kralove, Czech Republic; 2 Charles University- Medical Faculty and Faculty Hospital, Department of Research and Development, Hradec Kralove, Czech Republic Objectives: Use of standard enteral nutrition (EN) can cause hyperglycemia and associated complications. It may be recommended to use EN specially developed to minimize glycaemic response. On the other hand, these formulas often contain small amount of proteins, which could be a disadvantage. Methods: Eight type 2 diabetes mellitus patients treated with diet were involved. Three types of EN were compared: Nutrison MF (standard EN), Diben drink (regular hypocaloric diabetic nutrition) and Glucerna Select (isocaloric diabetic nutrition). We measured levels of blood glucose, free fatty acids (FFA) and Glucagon-like peptide-1 (GLP-1) at baseline and 15, 30, 45, 60, 90 and 120 minutes after ingestion of solution containing 25g of carbohydrates. Upward parts of the area under the curve (AUC) were compared. Measurements were repeated 3 times for each solution. Results: Statistical analysis was performed by One Way RM ANOVA. The glycemic index (GI) as a marker od postprandial glycemia of Nutrison MF was 62,5± 20,2, Diben drink 48,2±7,6 and Glucerna Select 28.1±7.5. Results of GI after Nutrison MF were statistically different from Glucerna Select (P <0.001). The plasma FFA after ingestion of Nutrison MF, Diben drink and Glucerna Select did not differ up to 60 minute. After that the plasma FFA remained significantly increased with Glucerna Select (P<0.001). The AUC of GLP-1 after ingestion of different types of EN were not statistically different. Conclusions: Our results indicate that diabetes-specific formulas of enteral nutrition could be beneficial for diabetic patients. This work was supported by Prvouk P37/12, IGA MH CR NT/12287-5
EAS16-0539, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. REGULATION OF HYPOTHALAMIC GENE EXPRESSION BY DNA METHYLATION IS INFLUENCED BY PARENTAL DIET DURING PREGNANCY AND LINKED TO OFFSPRING'S RISK OF OBESITY I. Khurana 1, A. Kaspi 1, M. Ziemann 1, T. Block 1, T. Connor 2, B. Spolding 2, A. Cooper 2, P. Zimmet 1, A. El-Osta 1, 3, 4, K. Walder 2. 1 Baker IDI Heart and Diabetes Institute, Human Epigenetics, Melbourne, Australia; 2 Faculty of Health- Medicine- Nursing and Behavioral Sciences- Deakin University,