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Dominant lethal test with trichlorphon in male mice
224 sampled at different times to determine the e x t e n t of chromosome damage. The mutagens investigated were ethyl methanesulphonate, m i t o m y c i n C, trimethy!phosphate, benzene and vinyl chloride, at single and/or multiple doses (5 daily). Various categories of chromosome damage were observed in all cases. However, the extent of damage due to chromosome and chromatid gaps was greater than or increased in parallel with other categories of damage. It has been suggested t h a t chromosome and chromatid gaps are indicative of toxic phenomena but this study suggests t h a t sucb aberrations may be useful and sensitive indicators of chemically induced genetic damage. In addition the study has also shown that single exposure may be as effective as multiple exposure in producing chromosome damage and that the correct sampling time is necessary to detect this damage.
111 K. Becket and J. SchSneich, Zentralinstitut fiir Genetik und Kulturpflanzenforschung der AdW der DDR, 4325 Gatersleben (German Democratic Republic) D o m i n a n t lethal test with trichlorphon in male mice Two groups of 20 male NMRI mice were injected i.p. by a single dose of 4.5 × 10 -2 M and 3 X 10 -: M trichlorphon, respectively. Each treated male was mated with 2 untreated females weekly for 8 successive weeks. All females were killed on the 13th to 17th day p.c. and their uteri were examined for preand post-implantation loss by counting corpora lutea, implantations, live and dead implants. According to our investigations, trichlorphon had n o mutagenic effect, in terms of the induction of d o m i n a n t lethal mutations, on germ cells of male NMRI mice in any stage of spermatogenesis.
112 J. Bogajewski, Institute o f Human Genetics of the Polish Academy of Sciences, 60 781 Poznah, ul. Swiecickiego 6 (Poland)
Procedure for estimation o f chromosome aberrations in anaphases in vivo in Ehrlich ascites turnout cells in mice Donor mice with transplanted Ehrlich ascites t u m o u r cells were injected with low concentration of colchicine. The exudate from the donor mouse was washed and transplanted into the recipient mouse. The exudate from the recipient mouse was collected in the time o f maximal n u m b e r of anaphases. The ascites cells were fixed and stained with acetic orcein. The o p t i m u m conditions were: dose of colchicine 85 X 10 -6 g/kg; time of the colchicine action 13 h; collection of the ascites cells from the donor mouse on the 6th day after transplantation o f the ascites cells to the donor mice; twofold washing of the ascites cells in Hanks' solution at 20°C; number of the transplanted cells to the recipient mouse 5 × 107 in 0.25 ml PBS; collections
111 K. Becket and J. SchSneich, Zentralinstitut fiir Genetik und Kulturpflanzenforschung der AdW der DDR, 4325 Gatersleben (German Democratic Republic) D o m i n a n t lethal test with trichlorphon in male mice Two groups of 20 male NMRI mice were injected i.p. by a single dose of 4.5 × 10 -2 M and 3 X 10 -: M trichlorphon, respectively. Each treated male was mated with 2 untreated females weekly for 8 successive weeks. All females were killed on the 13th to 17th day p.c. and their uteri were examined for preand post-implantation loss by counting corpora lutea, implantations, live and dead implants. According to our investigations, trichlorphon had n o mutagenic effect, in terms of the induction of d o m i n a n t lethal mutations, on germ cells of male NMRI mice in any stage of spermatogenesis.
112 J. Bogajewski, Institute o f Human Genetics of the Polish Academy of Sciences, 60 781 Poznah, ul. Swiecickiego 6 (Poland)
Procedure for estimation o f chromosome aberrations in anaphases in vivo in Ehrlich ascites turnout cells in mice Donor mice with transplanted Ehrlich ascites t u m o u r cells were injected with low concentration of colchicine. The exudate from the donor mouse was washed and transplanted into the recipient mouse. The exudate from the recipient mouse was collected in the time o f maximal n u m b e r of anaphases. The ascites cells were fixed and stained with acetic orcein. The o p t i m u m conditions were: dose of colchicine 85 X 10 -6 g/kg; time of the colchicine action 13 h; collection of the ascites cells from the donor mouse on the 6th day after transplantation o f the ascites cells to the donor mice; twofold washing of the ascites cells in Hanks' solution at 20°C; number of the transplanted cells to the recipient mouse 5 × 107 in 0.25 ml PBS; collections