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Doppler-ultrasonography in diagnosis of liver haemangioma
346
DOPPLER-ULTRASONOGRAPHY IN DIAGNOSIS OF LIVER HAEMANGIOMA M.Peri~i6_Savi6,R.Je~i6,V.Peri~i 6 Department of hepatology,Clinical center of Medical faculty Beogrsd,Yugoslsvia
Doppler-ultresonogrsphy can be used for various measurements of blood flow in abdominal blood vessels,and iu this work we have tried to exemiue characteristics of flow in liver haemaugiomas,using new doppler equipment (pmlsed wave probe, 5.75 MHz,Toshiba-lOOA).We have examined lO petients with proved liver hsemangioma(ultrssonography,blood-pool liver sciatigrephy,arteriogrsphy was done in ell patiMeuts).Dismeter of these vssculer tumours was 18 mm-60 mm.In small lesions( 20 mm or less),we could not find characteristic doppler flow effects.In greater lesions,particularly in gient hsemsngioma,we have found erterial blood flow and some chenges which may be called "turbulent" flow.These findings will be the fiild of our further investigations.We hope that this technique mey one day replace artex~grsphy in the diagnosis of haemangiome of the liver.
347
ENDOTHELIAL CELL SIDEROSIS IN HUMAN LIVER L M Petrovic, P J Scheuer Department of Histopathology, Royal Free Hospital and School of Medicine, Pond Street, London NW3 2QG, England
While the causes of iron deposition in hepatocytes and Kupffer cells are well studied, little is known about iron in the endothelium of the hepatic sinusoids, although recently siderosomes have been reported in these cells in acute hepatitis. We therefore studied 200 consecutive liver biopsies in order to determine the prevalence of endothelial siderosis and its relation to iron in other cell types. Paraffin sections were stained by Perls' method and iron semiquantitatively graded from O to 4+. The nature of the sinusoidal cells containing iron was confirmed using Ulex Europaeus i lectin and by electron-microscopy. Endothelial cells (EC) contained stainable iron in 58 (29%) biopsies. In 27 biopsies (13.5%) iron was present in EC, hepatocytes and Kupffer cells. In 19 biopsies (9.5%) there was iron in EC and Kupffer cells but not hepatocytes, while in 5 (2.5%) there was iron in EC and hepatocytes but not Kupffer cells. Overall 92 (46%) of biopsies showed iron in one or more cell types. The largest amounts of EC iron (grade 3 and 4) were found in 13 patients with known iron storage diseases. Decreasing amounts were seen in 9 patients with alcoholic liver disease, 2 patients with acute type B hepatitis, 4 with chronic active type B hepatitis and 3 with cholestasis. EC iron was also found in a variety of other diseases. We conclude that EC iron deposition is a relatively common finding in various liver diseases other than haemochromatosis. Its mechanisms and significance deserve further study.
$179
DOPPLER-ULTRASONOGRAPHY IN DIAGNOSIS OF LIVER HAEMANGIOMA M.Peri~i6_Savi6,R.Je~i6,V.Peri~i 6 Department of hepatology,Clinical center of Medical faculty Beogrsd,Yugoslsvia
Doppler-ultresonogrsphy can be used for various measurements of blood flow in abdominal blood vessels,and iu this work we have tried to exemiue characteristics of flow in liver haemaugiomas,using new doppler equipment (pmlsed wave probe, 5.75 MHz,Toshiba-lOOA).We have examined lO petients with proved liver hsemangioma(ultrssonography,blood-pool liver sciatigrephy,arteriogrsphy was done in ell patiMeuts).Dismeter of these vssculer tumours was 18 mm-60 mm.In small lesions( 20 mm or less),we could not find characteristic doppler flow effects.In greater lesions,particularly in gient hsemsngioma,we have found erterial blood flow and some chenges which may be called "turbulent" flow.These findings will be the fiild of our further investigations.We hope that this technique mey one day replace artex~grsphy in the diagnosis of haemangiome of the liver.
347
ENDOTHELIAL CELL SIDEROSIS IN HUMAN LIVER L M Petrovic, P J Scheuer Department of Histopathology, Royal Free Hospital and School of Medicine, Pond Street, London NW3 2QG, England
While the causes of iron deposition in hepatocytes and Kupffer cells are well studied, little is known about iron in the endothelium of the hepatic sinusoids, although recently siderosomes have been reported in these cells in acute hepatitis. We therefore studied 200 consecutive liver biopsies in order to determine the prevalence of endothelial siderosis and its relation to iron in other cell types. Paraffin sections were stained by Perls' method and iron semiquantitatively graded from O to 4+. The nature of the sinusoidal cells containing iron was confirmed using Ulex Europaeus i lectin and by electron-microscopy. Endothelial cells (EC) contained stainable iron in 58 (29%) biopsies. In 27 biopsies (13.5%) iron was present in EC, hepatocytes and Kupffer cells. In 19 biopsies (9.5%) there was iron in EC and Kupffer cells but not hepatocytes, while in 5 (2.5%) there was iron in EC and hepatocytes but not Kupffer cells. Overall 92 (46%) of biopsies showed iron in one or more cell types. The largest amounts of EC iron (grade 3 and 4) were found in 13 patients with known iron storage diseases. Decreasing amounts were seen in 9 patients with alcoholic liver disease, 2 patients with acute type B hepatitis, 4 with chronic active type B hepatitis and 3 with cholestasis. EC iron was also found in a variety of other diseases. We conclude that EC iron deposition is a relatively common finding in various liver diseases other than haemochromatosis. Its mechanisms and significance deserve further study.
$179