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Drug loaded nanodroplets penetration in dentinal tubules
d e n t a l m a t e r i a l s 3 0 S ( 2 0 1 4 ) e1–e180
e157
Results: Increased TDT of dentinal collagen after EDC treatment. Conclusion: CS aging treatment had no effect on collagen TDT, thus the first hypothesis was rejected. Conversely, the second hypothesis was accepted since EDC pretreatment increased collagen TDT in both groups 2 and 4, suggesting that EDC increases collagen toughness, requiring higher temperatures to denature the collagen matrix. Keywords: Thermal; Collagen; Dentine Groups
1 2 3 4
Static Static + EDC CS CS + EDC
T signal max (◦ C)
72.2 76.7 72.8 76.3
± ± ± ±
1.9 A 1.4 B 1.4 A 1.4 B
http://dx.doi.org/10.1016/j.dental.2014.08.319 319
http://dx.doi.org/10.1016/j.dental.2014.08.320
Drug loaded nanodroplets penetration in dentinal tubules
320
R. Tempesta ∗ , M. Cuppari, G. Ventura, M. Gai, C. Guiot, R. Cavalli, E. Berutti, N. Scotti
Osteoblast-cell behaviour on titanium-surfaces: An in-vitro analysis of protein-adsorption impact
University of Bologna, Italy Purpose: Drug loaded nanodroplets (DLNs) could be employed for MMPs inhibition agents delivery and release in dentin. The aim of this in vitro study was to evaluate the penetration of DLNs in dentinal tubules, under the hybrid layer of etch-and-rinse adhesive systems. Methods and materials: The occlusal third of sound human molars was removed and the exposed dentin surfaces were treated as follows: 36% phosphoric acid etching for 15 s, application of DLNs loaded with isothiocyanate fluorescein, application of Schotchbond Universal dyed with Rhodamine B. After composite build up, the specimens were stored for 24 h in distilled water at 37 ◦ C, longitudinally sectioned, and the generated interfaces were examined under confocal laser scanning microscopy (Leica TCS SP 8.0). A semi-quantitative analysis of the penetration depth of the DLNs and the adhesive resin into the dentinal tubules was performed utilizing FiJi software (WikyMedia, USA). Results: Mean penetration depth values of DLNs into dentinal tubules was 38.0366 m (DS 15.5578 m) Conclusion: Within the limits of this study we can conclude that DLNs can be applied prior to etch-and-rinse adhesive system on dentin. MMPs inhibitor agents release and effects must be further evaluated.
C. Parisi 1,2,∗ , R. Wazen 2 , A. Nanci 2,3 , G. Piana 1 1
Faculty of Dentistry, Alma Mater Studiorum, University of Bologna, Italy 2 Faculty of Dentistry, Université De Montréal, Canada 3 Department of Biochemistry and Molecular Medicine, Université De Montréal, Canada
Purpose: The insertion of dental implants is immediately followed by protein adsorption at the implant site. Despite numerous studies, it is still matter of debate at what extent this event can impact on cellular response, particularly when implant surfaces exhibit topography. To investigate this question, we compared the osteoblastic cell growth onto polished and nanoporous titanium and glass as control by modulating the exposure to serum proteins during the initial phase of cell culture. Methods and materials: Substrates consisted of: (1) commercial-grade-2 titanium disks polished to mirror finish, (2) polished disks nanotextured by treatment with H2 SO4 /H2 O2 for 2 h, and (3) glass coverslips as control (n = 24 for each). The substrates were pre-treated for 1 h with alphaMinimum Essential-Medium (aMEM) alone (MnoS) or aMEM supplemented with 10% foetal bovine serum (MS). Mouse calvaria derived osteoblastic cells (MC3T3-EI) were seeded on these pre-treated substrates and cultured for 24 h in MnoS and MS. Subsequently, cells were maintained in culture with MS for 7 days. Cell number was evaluated using Alamar-blue assay at days 1, 3 and 7. Division cell activity was determined by immunolabeling for Ki-67 nuclear-protein.
e157
Results: Increased TDT of dentinal collagen after EDC treatment. Conclusion: CS aging treatment had no effect on collagen TDT, thus the first hypothesis was rejected. Conversely, the second hypothesis was accepted since EDC pretreatment increased collagen TDT in both groups 2 and 4, suggesting that EDC increases collagen toughness, requiring higher temperatures to denature the collagen matrix. Keywords: Thermal; Collagen; Dentine Groups
1 2 3 4
Static Static + EDC CS CS + EDC
T signal max (◦ C)
72.2 76.7 72.8 76.3
± ± ± ±
1.9 A 1.4 B 1.4 A 1.4 B
http://dx.doi.org/10.1016/j.dental.2014.08.319 319
http://dx.doi.org/10.1016/j.dental.2014.08.320
Drug loaded nanodroplets penetration in dentinal tubules
320
R. Tempesta ∗ , M. Cuppari, G. Ventura, M. Gai, C. Guiot, R. Cavalli, E. Berutti, N. Scotti
Osteoblast-cell behaviour on titanium-surfaces: An in-vitro analysis of protein-adsorption impact
University of Bologna, Italy Purpose: Drug loaded nanodroplets (DLNs) could be employed for MMPs inhibition agents delivery and release in dentin. The aim of this in vitro study was to evaluate the penetration of DLNs in dentinal tubules, under the hybrid layer of etch-and-rinse adhesive systems. Methods and materials: The occlusal third of sound human molars was removed and the exposed dentin surfaces were treated as follows: 36% phosphoric acid etching for 15 s, application of DLNs loaded with isothiocyanate fluorescein, application of Schotchbond Universal dyed with Rhodamine B. After composite build up, the specimens were stored for 24 h in distilled water at 37 ◦ C, longitudinally sectioned, and the generated interfaces were examined under confocal laser scanning microscopy (Leica TCS SP 8.0). A semi-quantitative analysis of the penetration depth of the DLNs and the adhesive resin into the dentinal tubules was performed utilizing FiJi software (WikyMedia, USA). Results: Mean penetration depth values of DLNs into dentinal tubules was 38.0366 m (DS 15.5578 m) Conclusion: Within the limits of this study we can conclude that DLNs can be applied prior to etch-and-rinse adhesive system on dentin. MMPs inhibitor agents release and effects must be further evaluated.
C. Parisi 1,2,∗ , R. Wazen 2 , A. Nanci 2,3 , G. Piana 1 1
Faculty of Dentistry, Alma Mater Studiorum, University of Bologna, Italy 2 Faculty of Dentistry, Université De Montréal, Canada 3 Department of Biochemistry and Molecular Medicine, Université De Montréal, Canada
Purpose: The insertion of dental implants is immediately followed by protein adsorption at the implant site. Despite numerous studies, it is still matter of debate at what extent this event can impact on cellular response, particularly when implant surfaces exhibit topography. To investigate this question, we compared the osteoblastic cell growth onto polished and nanoporous titanium and glass as control by modulating the exposure to serum proteins during the initial phase of cell culture. Methods and materials: Substrates consisted of: (1) commercial-grade-2 titanium disks polished to mirror finish, (2) polished disks nanotextured by treatment with H2 SO4 /H2 O2 for 2 h, and (3) glass coverslips as control (n = 24 for each). The substrates were pre-treated for 1 h with alphaMinimum Essential-Medium (aMEM) alone (MnoS) or aMEM supplemented with 10% foetal bovine serum (MS). Mouse calvaria derived osteoblastic cells (MC3T3-EI) were seeded on these pre-treated substrates and cultured for 24 h in MnoS and MS. Subsequently, cells were maintained in culture with MS for 7 days. Cell number was evaluated using Alamar-blue assay at days 1, 3 and 7. Division cell activity was determined by immunolabeling for Ki-67 nuclear-protein.