- Email: [email protected]
Dual effect of cholestasis on the relaxation of corpus cavernosum in bile duct-ligated rats
Category 7: Transport, biliary disease, gallstones The terminal ductopenic stage PBC is characterized by the disappearance of ER-alpha in association with enhanced apoptosis and impaired cell proliferation. These data suggest that estrogens and their receptors are involved in the regulation of cholangiocyte proliferation/death in the corn--se of PBC.
I
637
THE IMPORTANCE LEVELS
OF ACYLATION-STIMULATING
PROTEIN
IN MALE PATIENTS WITH NONALCOHOLIC
185
between BDL and sham-operated animals, implying that the smooth muscle components of corporal relaxation were intact. N (w) -nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, abolished the relaxation to acetylcholine and EFS in both groups, suggesting that both kinds of relaxation were mediated mainly via NO. Our results show that cholestasis causes a dual alteration in the relaxation of corpus cavernosum: impairment in endothelium-dependent relaxation and enhancement in nitrergic transmission.
LIVER
DISEASES S. Bagci’,
Z. Yesilova’, A. Tuzun’, Y. Ates’, A. Uygun’, A. Erdil’, S. Tapan2, M. G&en’, N. Karaeren’, K. Dagalp’. ‘Department Of Gastroenterology, GATA, Ankara, Turkey; 2Department Of Biochemistry, GATA, Ankara, Turkey
Acylation-stimulating protein (ASP) is an adipocyte-derived protein that has been recently been suggested to play an important role in the regulation of lipoprotein metabolism and triglicerides storage. In this study, the serum ASP levels were investigated in male patients with nonalcoholic fatty liver disease (NAFLD) to investigate its possible role in the pathogenesis. 46 male patients with NAFLD diagnosed by biopsy and 30 healthy male enrolled in the study. Fasting plasma lipoproteins, triglicerides, total cholesterol, apolipoprotein A (Apo A), apolipoprotein B (APO B), complement C3, ASP, insulin and C-peptide levels were measured in both group. Additionally body mass index(BM1) was calculated in all subjects. The levels of cholesterol (p=O. 002), triglicerides (~‘0. OOl), LDL and VLDL (p=O. 029 and p
I 638
DUAL EFFECT CORPUS
OF CHOLESTASIS
CAVERNOSUM
ON THE RELAXATION
IN BILE DUCT-LIGATED
I
639
ROLE OF ERCP IN ACUTE CHOLANGITIS CLONORCHIS
SINENSIS
INFESTATIONS
STUDY WITH CONVENTIONAL
WITH HEAVY -COMPARISON
MEDICAL TREATMENT
l? Eun Taek, S. Sang Young, L. Sang Hyuk, C.H. Jung Myung. Gasterointestinal, Internal Medicine, Pusan Paik Hospital, Pusan, South Background: Clonorchis Sinensis(C.S.) infestation is well known that longstanding heavy infestation of C.S. in human might induce biliary tract diseases. The purpose of this study is to investigate role of ERCP in acute cholangitis with heavy C.S infestations. Methods: We assessed thirty three consecutive cases of acute cholangitis with heavy C.S. infestation which was confirmed by stool examination(>EPG 10,000) and ERCP findings of filling defects and dilatations in the peripheral bile duct during the period from Mar. 1995 to Jul. 2002. Fifteen patients(Group A) were received only conventional treatment (Praziquantel, 25mg/kg, three times a day for two days) the remainder(eighteen patients: Group B) was treated with Endoscopic Sphinterotomy (EST) and Naso-biliary drainage(ENBD) and then two days later, Anti-C.S. drug prescribed for two days. Results: The mean age of patients was sixty three year-old and male and female ratio was 16: 1. Seven patients of Group A, recurred symptomatic cholangitis and biochemical abnormality, but nobody occurred in Group B(p<0.005). We assessed recovery time of hematologic and biochemical findings between two groups. In Group A, mean time is twenty three days compare to in Group B seventeen days(P<0.005). For evaluation of improvement of bile duct, we checked abdominal ultrasonography two weeks later. Three patients of Group A, markedly improvement, compare to twelve patients of Group B(p
OF
RATS
M. Dehghani, H. Sadeghipour, A.R. Dehpour. Department Of Pharmacology, School Of Medicine, Tehran University Of Medical Sciences, Tehran, Iran Relaxation of the penile corpus cavernosum has an important role in penile erection which is regulated by local factors released from nerve fibers and sinusoidal endothelium. Nitric oxide (NO) is now accepted as the most important relaxant mediator in the process of erection which is produced in the endothelium and nitrergic nerves of corpus cavernosum. Previous studies have demonstrated endothelial dysfunction in vascular systems of cholestatic patients. This, together with evidence for increased nitrergic neurotransmission in anococcygeus muscles of cholestatic rats, suggested the possibility of an alteration in the function of corpus cavernosum of cholestatic rats. To study this, we used two main groups of rats: shamoperated and two-week bile duct-ligated (BDL). Strips of corpus cavernosum were mounted under tension in oxygenated organ bath. Following contraction with phenylephrine strips were relaxed by the addition of either acetylcholine or sodium nitroprusside. Electrical field stimulation (EFS) was performed in the presence of a&-opine and guanethidine. The results showed that relaxation responses to EFS were accentuated in BDL animals, whereas acetylcholine-induced endothelium-dependent relaxation was attenuated. Sodium nitroprusside-induced relaxation was not different
I640
ABILITY OF GLYCOURSODEOXYCHOLATE ASTROCYTE
INJURY BY BILIRUBIN
THE MEMBRANE
TO PREVENT
MAY BE RESTRICTED
PATHWAY-DEPENDENT
TO
CYTOTOXICITY
A. Femandes, A.S. Falcao, R.F. Silva, M.A. Brito, D. Brites. Unidade De Biologia Molecular E Biopatologia Experimental, Faculdade De Farmacia, University Of Lisbon, Lisbon, Portugal Background: With the reemergence of kernicterus there is an increasing necessity to identify new pathway (s) for bilirubin-induced neurotoxicity and to explore therapeutic options. In previous studies, we demonstrated that unconjugated bilirubin (UCB) directly interacts with membranes determining cell death, and that ursodeoxycholate is protective, probably by stabilizing membranes. Aims: To further characterize UCB-induced toxicity in astrocytes and to investigate the protective role of glycoursodeoxycholate. Methods: Astrocytes were isolated from 2-day-old Wistar rats and cultured for 10 days. Cells were incubated with 50 FM purified UCB (UCB/human albumin=0.5), for 4 h at 37°C. In sister cultures, cells were co-incubated with 50 FM glycoursodeoxycholate. Morphologic evaluation of apoptosis was performed by nuclear staining with Hoechst 33258. Release of TNF-a, LDH and glutamate into the culture medium was assessed by ELISA and commercial kits, and NO production by using Griess reagent.
I
637
THE IMPORTANCE LEVELS
OF ACYLATION-STIMULATING
PROTEIN
IN MALE PATIENTS WITH NONALCOHOLIC
185
between BDL and sham-operated animals, implying that the smooth muscle components of corporal relaxation were intact. N (w) -nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, abolished the relaxation to acetylcholine and EFS in both groups, suggesting that both kinds of relaxation were mediated mainly via NO. Our results show that cholestasis causes a dual alteration in the relaxation of corpus cavernosum: impairment in endothelium-dependent relaxation and enhancement in nitrergic transmission.
LIVER
DISEASES S. Bagci’,
Z. Yesilova’, A. Tuzun’, Y. Ates’, A. Uygun’, A. Erdil’, S. Tapan2, M. G&en’, N. Karaeren’, K. Dagalp’. ‘Department Of Gastroenterology, GATA, Ankara, Turkey; 2Department Of Biochemistry, GATA, Ankara, Turkey
Acylation-stimulating protein (ASP) is an adipocyte-derived protein that has been recently been suggested to play an important role in the regulation of lipoprotein metabolism and triglicerides storage. In this study, the serum ASP levels were investigated in male patients with nonalcoholic fatty liver disease (NAFLD) to investigate its possible role in the pathogenesis. 46 male patients with NAFLD diagnosed by biopsy and 30 healthy male enrolled in the study. Fasting plasma lipoproteins, triglicerides, total cholesterol, apolipoprotein A (Apo A), apolipoprotein B (APO B), complement C3, ASP, insulin and C-peptide levels were measured in both group. Additionally body mass index(BM1) was calculated in all subjects. The levels of cholesterol (p=O. 002), triglicerides (~‘0. OOl), LDL and VLDL (p=O. 029 and p
I 638
DUAL EFFECT CORPUS
OF CHOLESTASIS
CAVERNOSUM
ON THE RELAXATION
IN BILE DUCT-LIGATED
I
639
ROLE OF ERCP IN ACUTE CHOLANGITIS CLONORCHIS
SINENSIS
INFESTATIONS
STUDY WITH CONVENTIONAL
WITH HEAVY -COMPARISON
MEDICAL TREATMENT
l? Eun Taek, S. Sang Young, L. Sang Hyuk, C.H. Jung Myung. Gasterointestinal, Internal Medicine, Pusan Paik Hospital, Pusan, South Background: Clonorchis Sinensis(C.S.) infestation is well known that longstanding heavy infestation of C.S. in human might induce biliary tract diseases. The purpose of this study is to investigate role of ERCP in acute cholangitis with heavy C.S infestations. Methods: We assessed thirty three consecutive cases of acute cholangitis with heavy C.S. infestation which was confirmed by stool examination(>EPG 10,000) and ERCP findings of filling defects and dilatations in the peripheral bile duct during the period from Mar. 1995 to Jul. 2002. Fifteen patients(Group A) were received only conventional treatment (Praziquantel, 25mg/kg, three times a day for two days) the remainder(eighteen patients: Group B) was treated with Endoscopic Sphinterotomy (EST) and Naso-biliary drainage(ENBD) and then two days later, Anti-C.S. drug prescribed for two days. Results: The mean age of patients was sixty three year-old and male and female ratio was 16: 1. Seven patients of Group A, recurred symptomatic cholangitis and biochemical abnormality, but nobody occurred in Group B(p<0.005). We assessed recovery time of hematologic and biochemical findings between two groups. In Group A, mean time is twenty three days compare to in Group B seventeen days(P<0.005). For evaluation of improvement of bile duct, we checked abdominal ultrasonography two weeks later. Three patients of Group A, markedly improvement, compare to twelve patients of Group B(p
OF
RATS
M. Dehghani, H. Sadeghipour, A.R. Dehpour. Department Of Pharmacology, School Of Medicine, Tehran University Of Medical Sciences, Tehran, Iran Relaxation of the penile corpus cavernosum has an important role in penile erection which is regulated by local factors released from nerve fibers and sinusoidal endothelium. Nitric oxide (NO) is now accepted as the most important relaxant mediator in the process of erection which is produced in the endothelium and nitrergic nerves of corpus cavernosum. Previous studies have demonstrated endothelial dysfunction in vascular systems of cholestatic patients. This, together with evidence for increased nitrergic neurotransmission in anococcygeus muscles of cholestatic rats, suggested the possibility of an alteration in the function of corpus cavernosum of cholestatic rats. To study this, we used two main groups of rats: shamoperated and two-week bile duct-ligated (BDL). Strips of corpus cavernosum were mounted under tension in oxygenated organ bath. Following contraction with phenylephrine strips were relaxed by the addition of either acetylcholine or sodium nitroprusside. Electrical field stimulation (EFS) was performed in the presence of a&-opine and guanethidine. The results showed that relaxation responses to EFS were accentuated in BDL animals, whereas acetylcholine-induced endothelium-dependent relaxation was attenuated. Sodium nitroprusside-induced relaxation was not different
I640
ABILITY OF GLYCOURSODEOXYCHOLATE ASTROCYTE
INJURY BY BILIRUBIN
THE MEMBRANE
TO PREVENT
MAY BE RESTRICTED
PATHWAY-DEPENDENT
TO
CYTOTOXICITY
A. Femandes, A.S. Falcao, R.F. Silva, M.A. Brito, D. Brites. Unidade De Biologia Molecular E Biopatologia Experimental, Faculdade De Farmacia, University Of Lisbon, Lisbon, Portugal Background: With the reemergence of kernicterus there is an increasing necessity to identify new pathway (s) for bilirubin-induced neurotoxicity and to explore therapeutic options. In previous studies, we demonstrated that unconjugated bilirubin (UCB) directly interacts with membranes determining cell death, and that ursodeoxycholate is protective, probably by stabilizing membranes. Aims: To further characterize UCB-induced toxicity in astrocytes and to investigate the protective role of glycoursodeoxycholate. Methods: Astrocytes were isolated from 2-day-old Wistar rats and cultured for 10 days. Cells were incubated with 50 FM purified UCB (UCB/human albumin=0.5), for 4 h at 37°C. In sister cultures, cells were co-incubated with 50 FM glycoursodeoxycholate. Morphologic evaluation of apoptosis was performed by nuclear staining with Hoechst 33258. Release of TNF-a, LDH and glutamate into the culture medium was assessed by ELISA and commercial kits, and NO production by using Griess reagent.