- Email: [email protected]
Early biomarkers of chronic kidney disease in diabetic patients
e150
Abstracts / Atherosclerosis 252 (2016) e1ee196
hazard analysis was used to examine the relationship between bseline UAE and incident hypertension. Results: During mean follow-up period of 3.7 years, 315 subjects developed hypertension.Multivariate Cox proportional hazard analysis has shown that 2nd quartile group of UAE already showed significantly higher risk for incident hypertension (HR 1.59, 95%CI: 1.10-2.43) compared with 1st quartile group. There was a significant interaction between UAE and GFR for the risk of incident hypertension. In highest quartile group of eGFR(90ml/min/1.73m2), the UAE was a risk for incident hypertension but not in the third or lower eGFR groups. Conclusions: In the Japanese general population, very mild increase in UAE associated with hyperfiltration may antedate incident hypertension.
EAS16-0887, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: KIDNEY. EARLY BIOMARKERS OF CHRONIC KIDNEY DISEASE IN DIABETIC PATIENTS Z. Zuraeva 1, O. Mikhaleva 1, O. Vikulova 2, A. Ilyin 3, M. Shamkhalova 2, M. Shestakova 2. 1 I.M.Sechenov The First Moscow State Medical University, Department of Endocrinology and Diabetology of Pediatric Faculty, Moscow, Russia; 2 Endocrinology Research Center, Department of diabetic nephropathy, Moscow, Russia; 3 Endocrinology Research Center, Laboratory of Clinical Diagnostics, Moscow, Russia Objectives: Microalbuminuria (MA) is classical but not precise predictor of chronic kidney disease (CKD) in diabetes. For the purpose to find potential early risk markers we examined а panel of peptides in T1DM and T2DM patients. Methods: We examined 111 patients (T1DM/T2DM: 51/60) with AER<20 mg/L (n¼80); <199 mg/L (n¼18); 200 mg/L (n¼13). Biomarkers of kidney damage (collagen IV, nephrin, podocin, KIM-1, NGAL, uromodulin, TIMP-1) were measured by ELISA in the morning urine and fasting plasma; overnight AER by immunoturbidimetry assay; eGFR by standard MDRD formula. Statistical analyses performed by STATISTICA 8.0, p<0.05. Results: In T1DM podocin increased prior to MA [0.17; 0.08; 0.084; р<0,05]; collagen’s increase with CKD progression [3.86; 6.06; 8,663; р<0,05]. In plasma we observed cystatin C increase [1060;1022; 2391; р<0,05]; significant increase of TIMP-1 observed at NA stage [2632; 2347; 2008; р<0,05]. Positive correlation was observed between the collagen with AER (r¼0,48;p¼0,002) and serum creatinine (r¼0,51;p¼0,001), KIM-1 with eGFR (r¼0.41;p¼0.05), NGAL with AER (r¼0.44;p¼0.05), uromodulin with eGFR (r¼0,49; p¼0.05). In T2DM we observed significant distinctions in urine levels of podocin [0.192; 0.568; 0.084, р1-3, p2-3<0.05]; nephrin increase NAvs.MA [0.661; 0.904, р<0,05], KIM-1 increase NAvs.MA [373.02; 434.47; 1109.08, p<0.05], NGAL increase NAvs.MA [1.74;2.185;14.66, p<0.05]. Biomarkers also had positive correlation with standard markers: collagen with AER (r¼0,48; p¼0,002), cystatin C, podocin and uromodulin with eGFR (r¼0,48, p¼0,002; r¼0,39, p¼0,015; r¼0,49, p¼0.0002, respectively). Conclusions: The biomarkers observed prior to MA let to consider them as a promising assay for the preclinical diagnostics of diabetic CKD.
EAS16-0112, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: KIDNEY. CHOLESTEROL LEVELS AND GLOMERULAR FILTRATION RATE e IS THERE ANY ASSOCIATION?
included serum HDL, LDL, total cholesterol levels (HDL-c, LDL-c, Tc) and creatinine. Glomerular filtration rate (GFR) was estimated through Cockcroft-Gault formula. Systolic blood pressure values (SBP) and diabetes were also recorded. A simple statistical analysis with Pearson correlation was implemented with SPSS®[22]. Data was reworked without individuals with HbA1C>6.5 and SBP>140. Results: Average serum Tc was 194mg/dL, LDL-c 119mg/dL, average estimated GFR was 122mL/min. Tc and SBP were correlated with age (0,26mg/ dl and 0,6mmHg increase annually respectively, p¼0,000). GFR was negatively correlated with age (-0,587, p¼0,000, regression: -1,305 ml/ min/year). HDL and LDL have some impact in GFR in ages <65 (0,157ml/ min/mg for HDL and -0,77ml/min/mg for LDL). People who took cholesterol medication had higher CKD stages and were also older (p¼0,000 for both). With age stratification the relation between GFR and medication was lost. In reworked data HDL-c loses influence on GFR, on the other hand Tc gained a negative influence Conclusions: On our results cholesterol seem to have a role on GFR. Since medication had no impact on GFR we might consider the benefits of earlier primary prevention in order to avoid renal atherosclerosis.
EAS16-0622, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: KIDNEY. HIGH-DENSITY LIPOPROTEIN CHOLESTEROL LEVELS AND CONTRASTINDUCED ACUTE KIDNEY DISEASE IN PATIENTS UNDERGOING PERCUTANEOUS CORONARY INTERVENTION Y. Liu 1, L. Hua-Long 1, W. Dengxuan 1, Z. Jianbin 1, C. Shiqun 1, D. ChongYang 2, C. Ping-Yan 2, T. Ning 1, C. Ji-Yan 1. 1 Guangdong General Hospital/ Guangdong Academiy of Medical Sciences, Department of Cardiology, Guangzhou, China; 2 National Clinical Research Center for Kidney DiseaseState Key Laboratory of Organ Failure Research- Department of Biostatistics- School of Public Health and Tropical Medicine- Southern Medical University- Guangzhou- China, Department of Biostatistics, Guangzhou, China Objectives: Low high-density lipoprotein cholesterol (HDL-C) levels might be associated with better outcomes in patients undergoing percutaneous coronary intervention (PCI), however, few studies investigated the relationship between HDL-C levels and contrast-induced acute kidney disease (CI-AKI), which results in high mortality and morbidity. We aim at investigating whether the low HDL-C was associated with reduced incidence of CI-AKI. Methods: We measured HDL-C level at baseline in 1176 patients aged 18 years who treated with PCI between January 2010 and October 2012 according to the institutional protocol. The primary outcome was CI-AKI, defined as an increase in the serum creatinine level by 25% or 0.5 mg/ dL from baseline within 72 h of contrast exposure. Results: The cumulative incidence of CI-AKI was 10.0%. Baseline low HDLC level was not significantly associated with decrease CI-AKI (9.9% vs 10.5%; P¼0.795), as well as multivariate analysis (high HDL-C vs. low HDLC; adjusted odds ratio[OR], 1.07; 95% confidence interval [CI], 0.57~2.01; P¼0.840). Multivariate Cox analysis showed that low HDL-C might not attenuate mortality (high HDL-C vs. low HDL-C; adjusted hazard ratios, 1.25; P¼0.553). Conclusions: Low HD-C level might be not associated with decreased CIAKI in patients undergoing percutaneous coronary intervention, as well as mortality.
J. Frutuoso 1, M. Bourbon 2. 1 Unidade Local de Saúde do Baixo Alentejo, Internal Medicine, Carnaxide, Portugal; 2 Instituto Nacional de Saúde Dr. Ricardo Jorge, Cardiovascular Studies Department, Lisbon, Portugal Objectives: Chronic Kidney disease (CKD) is an health hazard that alone can reduce significantly life quality. When associated with other diseases it’s a major health risk. This study has the objective to study dyslipidemia influence in CKD. Methods: A retrospective study based on E_Cor data (questionnaire plus blood analysis of 1682 patients) was conducted. The collected variables
Ă
Abstracts / Atherosclerosis 252 (2016) e1ee196
hazard analysis was used to examine the relationship between bseline UAE and incident hypertension. Results: During mean follow-up period of 3.7 years, 315 subjects developed hypertension.Multivariate Cox proportional hazard analysis has shown that 2nd quartile group of UAE already showed significantly higher risk for incident hypertension (HR 1.59, 95%CI: 1.10-2.43) compared with 1st quartile group. There was a significant interaction between UAE and GFR for the risk of incident hypertension. In highest quartile group of eGFR(90ml/min/1.73m2), the UAE was a risk for incident hypertension but not in the third or lower eGFR groups. Conclusions: In the Japanese general population, very mild increase in UAE associated with hyperfiltration may antedate incident hypertension.
EAS16-0887, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: KIDNEY. EARLY BIOMARKERS OF CHRONIC KIDNEY DISEASE IN DIABETIC PATIENTS Z. Zuraeva 1, O. Mikhaleva 1, O. Vikulova 2, A. Ilyin 3, M. Shamkhalova 2, M. Shestakova 2. 1 I.M.Sechenov The First Moscow State Medical University, Department of Endocrinology and Diabetology of Pediatric Faculty, Moscow, Russia; 2 Endocrinology Research Center, Department of diabetic nephropathy, Moscow, Russia; 3 Endocrinology Research Center, Laboratory of Clinical Diagnostics, Moscow, Russia Objectives: Microalbuminuria (MA) is classical but not precise predictor of chronic kidney disease (CKD) in diabetes. For the purpose to find potential early risk markers we examined а panel of peptides in T1DM and T2DM patients. Methods: We examined 111 patients (T1DM/T2DM: 51/60) with AER<20 mg/L (n¼80); <199 mg/L (n¼18); 200 mg/L (n¼13). Biomarkers of kidney damage (collagen IV, nephrin, podocin, KIM-1, NGAL, uromodulin, TIMP-1) were measured by ELISA in the morning urine and fasting plasma; overnight AER by immunoturbidimetry assay; eGFR by standard MDRD formula. Statistical analyses performed by STATISTICA 8.0, p<0.05. Results: In T1DM podocin increased prior to MA [0.17; 0.08; 0.084; р<0,05]; collagen’s increase with CKD progression [3.86; 6.06; 8,663; р<0,05]. In plasma we observed cystatin C increase [1060;1022; 2391; р<0,05]; significant increase of TIMP-1 observed at NA stage [2632; 2347; 2008; р<0,05]. Positive correlation was observed between the collagen with AER (r¼0,48;p¼0,002) and serum creatinine (r¼0,51;p¼0,001), KIM-1 with eGFR (r¼0.41;p¼0.05), NGAL with AER (r¼0.44;p¼0.05), uromodulin with eGFR (r¼0,49; p¼0.05). In T2DM we observed significant distinctions in urine levels of podocin [0.192; 0.568; 0.084, р1-3, p2-3<0.05]; nephrin increase NAvs.MA [0.661; 0.904, р<0,05], KIM-1 increase NAvs.MA [373.02; 434.47; 1109.08, p<0.05], NGAL increase NAvs.MA [1.74;2.185;14.66, p<0.05]. Biomarkers also had positive correlation with standard markers: collagen with AER (r¼0,48; p¼0,002), cystatin C, podocin and uromodulin with eGFR (r¼0,48, p¼0,002; r¼0,39, p¼0,015; r¼0,49, p¼0.0002, respectively). Conclusions: The biomarkers observed prior to MA let to consider them as a promising assay for the preclinical diagnostics of diabetic CKD.
EAS16-0112, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: KIDNEY. CHOLESTEROL LEVELS AND GLOMERULAR FILTRATION RATE e IS THERE ANY ASSOCIATION?
included serum HDL, LDL, total cholesterol levels (HDL-c, LDL-c, Tc) and creatinine. Glomerular filtration rate (GFR) was estimated through Cockcroft-Gault formula. Systolic blood pressure values (SBP) and diabetes were also recorded. A simple statistical analysis with Pearson correlation was implemented with SPSS®[22]. Data was reworked without individuals with HbA1C>6.5 and SBP>140. Results: Average serum Tc was 194mg/dL, LDL-c 119mg/dL, average estimated GFR was 122mL/min. Tc and SBP were correlated with age (0,26mg/ dl and 0,6mmHg increase annually respectively, p¼0,000). GFR was negatively correlated with age (-0,587, p¼0,000, regression: -1,305 ml/ min/year). HDL and LDL have some impact in GFR in ages <65 (0,157ml/ min/mg for HDL and -0,77ml/min/mg for LDL). People who took cholesterol medication had higher CKD stages and were also older (p¼0,000 for both). With age stratification the relation between GFR and medication was lost. In reworked data HDL-c loses influence on GFR, on the other hand Tc gained a negative influence Conclusions: On our results cholesterol seem to have a role on GFR. Since medication had no impact on GFR we might consider the benefits of earlier primary prevention in order to avoid renal atherosclerosis.
EAS16-0622, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: KIDNEY. HIGH-DENSITY LIPOPROTEIN CHOLESTEROL LEVELS AND CONTRASTINDUCED ACUTE KIDNEY DISEASE IN PATIENTS UNDERGOING PERCUTANEOUS CORONARY INTERVENTION Y. Liu 1, L. Hua-Long 1, W. Dengxuan 1, Z. Jianbin 1, C. Shiqun 1, D. ChongYang 2, C. Ping-Yan 2, T. Ning 1, C. Ji-Yan 1. 1 Guangdong General Hospital/ Guangdong Academiy of Medical Sciences, Department of Cardiology, Guangzhou, China; 2 National Clinical Research Center for Kidney DiseaseState Key Laboratory of Organ Failure Research- Department of Biostatistics- School of Public Health and Tropical Medicine- Southern Medical University- Guangzhou- China, Department of Biostatistics, Guangzhou, China Objectives: Low high-density lipoprotein cholesterol (HDL-C) levels might be associated with better outcomes in patients undergoing percutaneous coronary intervention (PCI), however, few studies investigated the relationship between HDL-C levels and contrast-induced acute kidney disease (CI-AKI), which results in high mortality and morbidity. We aim at investigating whether the low HDL-C was associated with reduced incidence of CI-AKI. Methods: We measured HDL-C level at baseline in 1176 patients aged 18 years who treated with PCI between January 2010 and October 2012 according to the institutional protocol. The primary outcome was CI-AKI, defined as an increase in the serum creatinine level by 25% or 0.5 mg/ dL from baseline within 72 h of contrast exposure. Results: The cumulative incidence of CI-AKI was 10.0%. Baseline low HDLC level was not significantly associated with decrease CI-AKI (9.9% vs 10.5%; P¼0.795), as well as multivariate analysis (high HDL-C vs. low HDLC; adjusted odds ratio[OR], 1.07; 95% confidence interval [CI], 0.57~2.01; P¼0.840). Multivariate Cox analysis showed that low HDL-C might not attenuate mortality (high HDL-C vs. low HDL-C; adjusted hazard ratios, 1.25; P¼0.553). Conclusions: Low HD-C level might be not associated with decreased CIAKI in patients undergoing percutaneous coronary intervention, as well as mortality.
J. Frutuoso 1, M. Bourbon 2. 1 Unidade Local de Saúde do Baixo Alentejo, Internal Medicine, Carnaxide, Portugal; 2 Instituto Nacional de Saúde Dr. Ricardo Jorge, Cardiovascular Studies Department, Lisbon, Portugal Objectives: Chronic Kidney disease (CKD) is an health hazard that alone can reduce significantly life quality. When associated with other diseases it’s a major health risk. This study has the objective to study dyslipidemia influence in CKD. Methods: A retrospective study based on E_Cor data (questionnaire plus blood analysis of 1682 patients) was conducted. The collected variables
Ă