EARLY DIAGNOSIS OF WOUND INFECTION

" " " " " " " " " ORIGINAL ARTICLES [MARCH 20, 1943 couldbe XBD=2364 XBD=1443 XBD=1645 XBD=1713 XBD=1934 XBD=2028 XBD=2161 XHG=1378 XHG=1474 XHG=16...

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ORIGINAL ARTICLES

[MARCH 20, 1943 couldbe

XBD=2364 XBD=1443 XBD=1645 XBD=1713 XBD=1934 XBD=2028 XBD=2161 XHG=1378 XHG=1474 XHG=1674 XHG=1739 XHG=1993 XHG=2054 XHG=2189 YBD=263OF YBD=255 YBD=253 YBD=254 YBD=262 YHG=225>the YHG=234>progress YHG=224>of YHG=223>infection. YHG=223>If YHG=223>these YHG=233>enzymes DIAGNOSIS WOUND INFECTION EARLY XBD=1557 XBD=1622 XBD=1697 XBD=1821 XBD=1965 XBD=2054 XBD=2296 XBD=2446 XBD=2580 XHG=1381 XHG=1579 XHG=1645 XHG=1719 XHG=1845 XHG=1988 XHG=2079 XHG=2320 XHG=2469 YBD=295 YHG=260>be-of YBD=302 YBD=293 YBD=300 YBD=301 YBD=294 YHG=264>detected YHG=264>at YHG=274>an YHG=265>early YHG=263>stage, YHG=263>the YHG=263>knowledge YHG=264>might REFERENCE TO GAS-GANGRENE SPECIAL XBD=1589 WITH XBD=1662 XBD=1756 XBD=1945 XBD=2014 XBD=2241 XBD=2291 XBD=2429 XBD=2581 XHG=1379 XHG=1617 XHG=1689 XHG=1785 XHG=1972 XHG=2039 XHG=2267 XHG=2319 XHG=2448 YBD=333 YBD=340 YBD=332 YBD=341 YBD=334 YHG=303>assistance YHG=304>to YHG=304>the YHG=313>surgeon YHG=302>in YHG=302>providing YHG=312>a YHG=304>rapid YHG=312>means XBD=1419 XHG=2321 XBD=1543 XBD=1771 XBD=1894 XBD=2046 XBD=2300 XBD=2580 XHG=1381 XHG=1440 XHG=1565 XHG=1817 XHG=1916 XHG=2066 YBD=381 YBD=379 YBD=373 YHG=343>of YHG=343>early YHG=342>diagnosis. YHG=343>The YHG=342>earlier YHG=341>recognition YHG=342>of anaerobic MB LOND H. J. ROGERS, B SC LOND D. McCLEAN, XBD=1565 XBD=1672 XBD=1770 XBD=1905 XBD=1963 XBD=2082 XBD=2276 XBD=2372 XBD=2579 XHG=1379 XHG=1581 XHG=1689 XHG=1787 XHG=1921 XHG=1979 XHG=2100 XHG=2293 XHG=2389 YBD=420 YHG=382>objective YBD=413 YBD=412 YBD=411 YBD=419 YHG=381>infection YHG=392>may YHG=383>also YHG=383>result YHG=383>in YHG=391>more YHG=381>vigorous YHG=381>and OF THE LISTER INSTITUTE OF PREVENTIVE MEDICINE, XBD=1576 XHG=2411 XBD=1760 XBD=1861 XBD=1954 XBD=2172 XBD=2388 XBD=2582 XHG=1378 XHG=1596 XHG=1783 XHG=1882 XHG=1976 XHG=2195 YBD=460 YBD=459 YBD=452 YHG=421>antitoxin YHG=422>therapy YHG=423>and YHG=423>less YHG=421>extensive YHG=420>operative YHG=421>excision ELSTREE, HERTS XBD=1474 XHG=2501 XBD=1531 XBD=1799 XBD=2032 XBD=2104 XBD=2234 XBD=2468 XBD=2580 XHG=1378 YBD=491 XHG=1507 XHG=1567 XHG=1832 XHG=2062 XHG=2137 XHG=2267 YBD=499 YBD=492 YBD=490 YBD=498 YHG=461>with YHG=470>a YHG=461>consequent YHG=461>reduction YHG=461>in YHG=460>both YHG=460>mortality YHG=461>and B M OXFD, F R C S B. W. WILLIAMS, XBD=1615 XHG=2601 XBD=1759 XBD=1904 XBD=2109 XBD=2162 XBD=2288 XBD=2513 XBD=2579 XBD=2606 XHG=1379 XHG=1666 XHG=1788 XHG=1936 XHG=2137 XHG=2192 XHG=2319 XHG=2540 YBD=531 YHG=498>‘ YBD=539 YBD=532 YBD=537 YBD=530 YBD=503 YHG=500>mutilation. YHG=501>This YHG=510>paper YHG=500>contains YHG=510>a YHG=498>brief YHG=508>summary YHG=501>of THOMAS’S HOSPITAL EMS SURGEON, XBD=1445 XHG=2540 XBD=1616 XBD=1689 XBD=1826 XBD=2114 XBD=2224 XBD=2507 XBD=2590 XHG=1378 XHG=1477 XHG=1650 XHG=1719 XHG=1859 XHG=2145 XHG=2257 YBD=569ST.YHG=540>of YBD=578 YBD=577 YBD=570 YHG=538>the YHG=540>results YHG=539>of YHG=539>these YHG=540>experiments YHG=539>and YHG=538>descriptions

" XBD=2579 XBD=1446 XHG=2396 XBD=1583 XBD=1739 XBD=1806 XBD=1872 XBD=2192 XBD=2362 XBD=2443 XHG=2477 XHG=1380 XHG=1483 XHG=1614 XHG=1772 XHG=1838 XHG=1904 XHG=2226 YBD=608section YBD=610 YBD=609 YHG=578>the YHG=578>tests YHG=579>which YHG=578>it YHG=579>is YHG=580>recommended YHG=578>should YHG=579>be YHG=579>tried With a technical by C. W. HALE

" XBD=2350 XBD=1419 XHG=2180 XBD=1503 XBD=1628 XBD=1840 XBD=1900 XBD=2103 XBD=2163 XHG=1377 YBD=650 XHG=1437 XHG=1525 XHG=1647 XHG=1861 XHG=1918 XHG=2121 YBD=647 YHG=618>wounds. YBD=656 YBD=649 YHG=617>in YHG=617>the YHG=618>early YHG=619>diagnosis YHG=618>of YHG=618>infection YHG=619>in

" XBD=1231 XBD=1316XHG=717 XBD=275 XBD=580 XBD=698 XBD=855 XBD=1076 XBD=1167 XHG=200 XHG=292 XHG=598 XHG=875YBD=693 XHG=1097 XHG=1188 XHG=1250 YBD=695 YBD=699 YBD=691 YBD=691 YBD=691YHG=662>wound YBD=692 YHG=665>THE YHG=661>case-mortality YHG=661>from YHG=660>infections YHG=655>due YHG=661>to YHG=661>the XBD=396 XHG=411 XBD=617 XBD=796 XBD=1041 XBD=1096 XBD=1175 XBD=1318 XHG=631 XHG=159 XHG=811YBD=731 XHG=1054 XHG=1108 XHG=1189 YBD=744 YBD=739 YBD=739 YBD=731YHG=701>remains YBD=738 YHG=711>gas-gangrene YHG=701>organisms YHG=700>obstinately YHG=701>in YHG=702>the YHG=702>region " XBD=2235 XBD=2032 XHG=1740 XHG=2052 YBD=745 YBD=740 YHG=709>Experimental YHG=710>Methods

"" XBD=1317 XBD=191 XBD=310 XBD=389 XBD=504 XBD=602 XBD=685 XBD=807 XBD=932 XBD=990 XBD=1191 XHG=144 XHG=207 XHG=327 XHG=408 XHG=555 XHG=617 XHG=704 XHG=825 XHG=1007 XHG=1206 YBD=775 YBD=774 YBD=780 YBD=772 YBD=770 YBD=779 YBD=778 YBD=771 YBD=771 YHG=730>of YHG=742>30-60 YHG=743>per YHG=741>cent. YHG=741>In YHG=740>the YHG=740>early YHG=740>stage YHG=740>of YHG=740>infection YHG=741>when XBD=1576XHG=951 XBD=1633 XBD=1872 XBD=1984 XBD=2170 XBD=2520 XBD=2579 XHG=1428 XHG=1594 XHG=1652 XHG=1889 XHG=2002 XHG=2185 XHG=2537 YBD=792 YBD=801 YBD=795 YBD=802 YBD=794 YHG=763>Groups YHG=763>of YHG=763>guineapigs YHG=773>were YHG=764>infected YHG=765>intramuscularly YHG=764>in " "" "

XBD=1318 XBD=211 XBD=366 XBD=458 XBD=571 XBD=758 XBD=830 XBD=1039 XBD=1137 XHG=160 XHG=239 XHG=396 XHG=489 XHG=602 XHG=792 XHG=862 XHG=1070 XHG=1171 YBD=814 YBD=812 YBD=818 YBD=810 YBD=818 YBD=810 YHG=784>the YHG=782>tissues YHG=790>are YHG=779>still YHG=780>capable YHG=780>of YHG=790>recovery YHG=780>the YHG=780>clinical XBD=1442 XBD=1572 XBD=1685 XBD=1852 XBD=2026 XBD=2207 XBD=2264 XBD=2357 XBD=2580 XHG=1376 XHG=1459 XHG=1588 XHG=1700 XHG=1872 XHG=2044 XHG=2225 XHG=2289 XHG=2386 YBD=832 YBD=839 YBD=833 YBD=835 YBD=834 YBD=841 YHG=801>the YHG=802>thigh YHG=802>with YHG=803>washed YHG=803>18-hour YHG=804>cultures YHG=804>of YHG=804>the YHG=803>organism XBD=298 XBD=391 XBD=470 XBD=622 XBD=761 XBD=895 XBD=1025 XBD=1315 XHG=158 XHG=331 XHG=429 XHG=506 XHG=658 XHG=930 XHG=1063 YBD=859 YBD=850 YBD=856 YBD=849 YBD=850 YBD=850 YHG=824>changes YHG=828>are YHG=828>so YHG=819>slight YHG=819>that, YHG=829>even YHG=820>with YHG=820>considerable XBD=1496XHG=799 XBD=1601 XHG=1376 XHG=1511 YBD=871 YBD=873 YHG=841>under YHG=841>test.

" XBD=1317 XBD=361 XHG=795 XBD=459 XBD=654 XBD=761 XBD=938 XBD=1069 XBD=1127 XHG=158 XHG=393 XHG=493 XHG=689 XHG=970YBD=889 XHG=1103 XHG=1164 YBD=898 YBD=896 YBD=889 YBD=889YHG=859>seldom YHG=860>experience, YHG=857>the YHG=867>surgeon YHG=868>can YHG=859>form YHG=869>a YHG=859>definite " XBD=1495 XBD=1663 XBD=1767 XBD=1884 XBD=1937 XBD=2180 XBD=2295 XBD=2488 XHG=1422 XHG=1510 XHG=1678 XHG=1782 XHG=1900 XHG=1952 XHG=2194 XHG=2309 XHG=2518 YBD=923 YBD=924 YBD=926 YBD=930 YBD=925 YHG=895>The YHG=895>cultures YHG=905>were YHG=896>made YHG=896>in YHG=896>Robertson’s YHG=897>heart YHG=897>medium YHG=897>the ; " XBD=2580 XBD=292 XHG=747 XBD=402 XBD=481 XBD=673 XBD=730 XBD=798 XBD=1012 XBD=1219 XBD=1318 XHG=158 XHG=306 XHG=416 XHG=498 XHG=691 XHG=812YBD=929 XHG=1026 XHG=1270 YBD=938 YBD=935 YBD=928 YBD=929 YBD=930YHG=907>an YBD=929 YHG=900>opinion YHG=897>as-to YHG=898>the YHG=907>presence YHG=899>of YHG=899>anaerobic YHG=898>infection. YHG=899>In " XBD=2581 XBD=1569 XBD=1675 XBD=1922 XBD=2081 XBD=2183 XBD=2238 XBD=2363 XBD=2454 XHG=1377 XHG=1586 XHG=1695 XHG=1940 XHG=2099 XHG=2201 XHG=2256 XHG=2382 XHG=2471 YBD=971 YBD=972 YBD=964 YBD=966 YBD=965 YHG=935>organisms YHG=944>were YHG=935>centrifuged, YHG=935>washed YHG=945>once YHG=937>in YHG=936>saline YHG=936>and YHG=937>taken XBD=237 XHG=660 XBD=374 XBD=578 XBD=640 XBD=771 XBD=1092 XBD=1316 XHG=157 XHG=257 XHG=396 XHG=601 XHG=792YBD=976 XHG=1113 YBD=970 YBD=975 YBD=967 YBD=975 YBD=976 YHG=940>view YHG=937>of’the YHG=937>difficulty YHG=938>of YHG=937>rapid YHG=937>bacteriological YHG=938>diagnosis, XBD=1425 XBD=1493 XBD=1633 XBD=1770 XBD=1838 XBD=1968 XBD=2160 XBD=2280 XBD=2462 XHG=1377 XHG=1456 XHG=1522 XHG=1663 XHG=1800 XHG=1867 XHG=1998 XHG=2207 XHG=2309 XHG=2491 YBD=1011 YBD=1002 YBD=1003 YBD=1013 YBD=1004 YBD=1005 YHG=983>up YHG=974>in YHG=975>either YHG=975>saline YHG=984>or YHG=975>CaCl2 YHG=975>solution. YHG=976>The YHG=976>cultures YHG=985>were " XBD=2581 XBD=182 XHG=813 XBD=327 XBD=457 XBD=579 XBD=635 XBD=800 XBD=880 XBD=1109 XBD=1163 XBD=1318 XHG=155 XHG=193 XHG=338 XHG=468 XHG=591 XHG=649 XHG=894YBD=1007 XHG=1123 XHG=1177 YBD=1010 YBD=1009 YBD=1008 YBD=1015 YBD=1016 YBD=1007YHG=977>the YBD=1008 YHG=979>it YHG=977>seemed YHG=976>worth YHG=976>while YHG=977>to YHG=977>explore YHG=977>possibility YHG=978>of YHG=977>detect" XBD=2586 XBD=1520 XBD=1576 XBD=1698 XBD=1756 XBD=1892 XBD=1947 XBD=2021 XBD=2080 XBD=2259 XBD=2335 XBD=2525 XHG=1377 XHG=1539 XHG=1596 XHG=1718 XHG=1777 XHG=1909 XHG=1967 XHG=2041 XHG=2099 XHG=2273 XHG=2355 XHG=2545 YBD=1050 YBD=1042 YBD=1052 YBD=1051 YBD=1043 YHG=1013>washed YHG=1014>in YHG=1014>order YHG=1017>to YHG=1014>avoid, YHG=1014>far YHG=1024>as YHG=1015>possible, YHG=1014>the YHG=1015>injection YHG=1015>of XBD=211 XHG=602 XBD=298 XBD=497 XBD=571 XBD=769 XBD=1061 XBD=1319 XHG=155 XHG=236 XHG=329 XHG=532 XHG=801YBD=1055 XHG=1092 YBD=1056 YBD=1047 YBD=1046 YBD=1054 YBD=1055YHG=1016>actively YHG=1019>ing YHG=1017>the YHG=1025>presence YHG=1017>of YHG=1017>proliferating YHG=1017>pathogenic XBD=1576 XBD=1699 XBD=1758 XBD=2056 XBD=2148 XBD=2267 XBD=2523 XHG=1377 XHG=1596 XHG=1719 XHG=1778 XHG=2103 XHG=2167 XHG=2288 XHG=2544 YBD=1091 YBD=1082 YBD=1090 YBD=1092 YBD=1083 YHG=1053>preformed YHG=1053>toxin YHG=1063>or YHG=1046>hyaluronidase. YHG=1056>In YHG=1055>those YHG=1055>experiments YHG=1055>in " XBD=2581 XBD=342 XHG=783 XBD=413 XBD=469 XBD=608 XBD=753 XBD=850 XBD=1065 XBD=1238 XBD=1316 XHG=156 XHG=371 XHG=445 XHG=501 XHG=642 XHG=883YBD=1087 XHG=1098 XHG=1271 YBD=1086 YBD=1094 YBD=1087 YBD=1088 YBD=1087 YHG=1056>organisms YHG=1055>at YHG=1065>a YHG=1056>stage YHG=1056>when YHG=1056>the YHG=1056>infection YHG=1058>cannot YHG=1057>be " XBD=2582 XBD=1492 XBD=1618 XBD=1712 XBD=1823 XBD=1906 XBD=2191 XBD=2254 XBD=2344 XBD=2436 XBD=2498 XHG=1378 XHG=1516 XHG=1641 XHG=1736 XHG=1845 XHG=1929 XHG=2214 XHG=2273 XHG=2368 XHG=2460 XHG=2520 YBD=1131 YBD=1123 YBD=1122 YHG=1093>which YHG=1093>CaC12 YHG=1103>was YHG=1094>used YHG=1094>concentration YHG=1094>of YHG=1094>this YHG=1094>salt YHG=1094>in YHG=1094>the XBD=348 XHG=629 XBD=408 XBD=565 XBD=617 XBD=807 XBD=1117 XBD=1319 XHG=154 XHG=358 XHG=419 XHG=576 XHG=818YBD=1134 XHG=1127 YBD=1126 YBD=1134 YBD=1127 YHG=1095>recognised YHG=1095>by YHG=1095>clinical YHG=1105>or YHG=1095>ordinary YHG=1096>bacteriological YHG=1097>examinaorward |>" XBD=1592 XBD=1771 XBD=1865 XBD=1911 XBD=2005 XBD=2069 XBD=2154 XBD=2261 XBD=2326 XBD=2488 XBD=2583 XBD=2401 XHG=1379 XHG=1617 XHG=1794 XHG=1893 XHG=1936 XHG=2031 XHG=2092 XHG=2179 XHG=2285 XHG=2427 XHG=2537 XHG=2348 YBD=1170 YBD=1169 YBD=1161 YBD=1162 YBD=1162 YHG=1133>suspending YHG=1132>solution YHG=1142>was YHG=1134>8 YHG=1142>mg. YHG=1134>in YHG=1134>the YHG=1134>dose YHG=1133>of YHG=1133>ml. YHG=1133>In YHG=1134>0-1 XBD=236 XBD=355 XBD=552 XBD=646 XHG=154 XHG=282 XHG=378 XHG=574 YBD=1165 YBD=1173 YHG=1137>tion. YHG=1135>The YHG=1135>methods YHG=1135>put " XBD=896 XBD=982 XBD=1197 XBD=1319 XHG=854 XHG=916 XHG=1005 XHG=1220 YBD=1166 YBD=1174 YBD=1166 YHG=1136>in YHG=1136>the YHG=1136>following YHG=1145>com" XBD=2582 XBD=1481 XBD=1734 XBD=1815 XBD=1983 XBD=2073 XBD=2243 XBD=2344 XBD=2402 XBD=2524 XHG=1381 XHG=1501 XHG=1755 XHG=1835 XHG=2001 XHG=2093 XHG=2261 XHG=2364 XHG=2422 XHG=2544 YBD=1209 YBD=1200 YBD=1213 YBD=1207 YBD=1202 YBD=1201 YHG=1172>other YHG=1172>experiments YHG=1172>the YHG=1172>calcium YHG=1182>was YHG=1173>omitted YHG=1172>and, YHG=1172>in YHG=1173>order YHG=1176>to XBD=369 XHG=731 XBD=553 XBD=696 XBD=799 XBD=1031 XBD=1107 XBD=1319 XHG=155 XHG=407 XHG=590 XHG=839YBD=1205 XHG=1068 XHG=1145 YBD=1210 YBD=1213 YBD=1205 YHG=1171>munication YHG=1174>depend YHG=1178>upon YHG=1175>the YHG=1175>detection YHG=1175>of YHG=1185>enzymes " XBD=2584 XBD=1543 XBD=1599 XBD=1714 XBD=1855 XBD=1935 XBD=2131 XBD=2330 XBD=2501 XHG=1380 XHG=1562 XHG=1619 XHG=1734 XHG=1875 XHG=1954 XHG=2151 XHG=2348 XHG=2521 YBD=1248 YBD=1246 YBD=1240 YHG=1212>simulate YHG=1213>in YHG=1221>some YHG=1212>degree YHG=1212>the YHG=1212>naturally YHG=1205>occurring YHG=1214>trauma, YHG=1211>the XBD=326 XHG=801 XBD=405 XBD=603 XBD=675 XBD=770 XBD=940 XBD=1145 XBD=1319 XHG=155 XHG=354 XHG=435 XHG=632 XHG=704 XHG=969YBD=1245 XHG=1201 YBD=1253 YBD=1251 YBD=1244 YBD=1246 YBD=1245YHG=1215>wound YHG=1213>produced YHG=1213>by YHG=1214>bacteria YHG=1214>in YHG=1214>the YHG=1214>exudate. YHG=1216>These " XBD=2583 XBD=1528 XBD=1634 XBD=1801 XBD=1903 XBD=2188 XBD=2444 XHG=1380 XHG=1546 XHG=1653 XHG=1817 XHG=1921 XHG=2206 XHG=2463 YBD=1280 YBD=1288 YBD=1287 YBD=1279 YHG=1251>muscles YHG=1261>were YHG=1251>crushed YHG=1251>with YHG=1250>artery-forceps YHG=1250>immediately YHG=1250>before XBD=310 XHG=934 XBD=388 XBD=632 XBD=843 XBD=915 XBD=958 XBD=1077 XBD=1320 XHG=154 XHG=327 XHG=406 XHG=651 XHG=863 XHG=976YBD=1284 XHG=1097 YBD=1283 YBD=1291 YBD=1292 YBD=1291 YBD=1292 YHG=1261>enzymes YHG=1261>are YHG=1253>apparently YHG=1253>produced YHG=1255>by YHG=1263>a YHG=1255>large YHG=1254>proportion " XBD=2024 XBD=1549 XBD=1606 XBD=1682 XBD=1834 XBD=1889 XHG=1380 XHG=1566 XHG=1620 XHG=1700 XHG=1852 XHG=1907 YBD=1327 YBD=1318 YBD=1319 YHG=1290>injection YHG=1290>of YHG=1290>the YHG=1290>culture YHG=1291>in YHG=1290>saline. XBD=188 XHG=898 XBD=532 XBD=768 XBD=872 XBD=968 XBD=1040 XBD=1243 XBD=1321 XHG=154 XHG=209 XHG=558 XHG=794 XHG=994YBD=1324 XHG=1069 XHG=1269 YBD=1330 YBD=1323 YBD=1323 YBD=1324YHG=1303>can YBD=1331 YHG=1293>of YHG=1291>wound-infecting YHG=1293>organisms YHG=1293>and YHG=1294>be YHG=1293>detected YHG=1294>by " XBD=2583 XBD=272 XHG=754 XBD=399 XBD=535 XBD=728 XBD=967 XBD=1053 XBD=1182 XBD=1321 XHG=153 XHG=294 XHG=448 XHG=552 XHG=990YBD=1363 XHG=1080 XHG=1204 YBD=1369 YBD=1370 YBD=1363 YBD=1363YHG=1332>advocated YBD=1371 YHG=1331>simple YHG=1330>tests. YHG=1332>The YHG=1332>methods YHG=1342>are YHG=1333>most YHG=1332>likely " XBD=1509 XBD=1753 XBD=1868 XBD=2011 XBD=2072 XBD=2193 XBD=2258 XBD=2462 XHG=1431 XHG=1534 XHG=1773 XHG=1892 XHG=2030 XHG=2092 XHG=2215 XHG=2278 XHG=2483 YBD=1382 YBD=1375 YBD=1373 YBD=1381 YBD=1374 YHG=1345>The YHG=1344>guineapigs YHG=1354>were YHG=1343>killed YHG=1343>in YHG=1344>pairs YHG=1344>at YHG=1343>intervals YHG=1343>after "" XBD=1322 XBD=190 XBD=248 XBD=382 XBD=440 XBD=513 XBD=673 XBD=893 XBD=1033 XBD=1113 XHG=153 XHG=206 XHG=266 XHG=398 XHG=459 XHG=530 XHG=909 XHG=1051 XHG=1134 YBD=1401 YBD=1402 YBD=1403 YBD=1404 YBD=1403 YBD=1411 YHG=1372>to YHG=1371>be YHG=1371>useful YHG=1371>in YHG=1371>Ol. YHG=1368>welcMi YHG=1372>infections YHG=1372>which YHG=1382>are YHG=1373>clinically XBD=1568XHG=690 XBD=1749 XBD=1866 XBD=1907 XBD=1971 XBD=2038 XBD=2182 XBD=2455 XBD=2581 XHG=1382 XHG=1587 XHG=1767 XHG=1886 XHG=1927 XHG=1991 XHG=2056 XHG=2230 XHG=2476 YBD=1415 YBD=1422 YBD=1412 YBD=1414 YBD=1413 YBD=1421 YHG=1384>infection YHG=1384>varying YHG=1384>from YHG=1383>2 YHG=1383>to YHG=1383>24 YHG=1383>hours. YHG=1383>Immediate YHG=1383>post"" XBD=1321 XBD=211 XBD=320 XBD=441 XBD=533 XBD=651 XBD=842 XBD=967 XBD=1024 XHG=153 XHG=225 XHG=333 XHG=456 XHG=548 XHG=668 XHG=985 XHG=1040 YBD=1441 YBD=1449 YBD=1442 YBD=1443 YBD=1442 YBD=1450 YHG=1411>the YHG=1410>most YHG=1410>rapid YHG=1412>and YHG=1412>most YHG=1421>common YHG=1422>cause YHG=1412>of YHG=1421>gas-gangrene. XBD=1547XHG=858 XBD=1872 XBD=2008 XBD=2175 XBD=2284 XBD=2583 XHG=1383 XHG=1588 XHG=1913 XHG=2051 XHG=2217 XHG=2327 YBD=1454 YBD=1453 YBD=1458 YBD=1452 YBD=1460 YHG=1424>mortem YHG=1423>examinations YHG=1432>were YHG=1423>made, YHG=1423>the YHG=1423>macroscopic that 9 15 XBD=230 XBD=306 XBD=411 XBD=561 XBD=631 XBD=749 XBD=941 XHG=197 XHG=241 XHG=318 XHG=423 XHG=572 XHG=646 XHG=762 YBD=1480 YBD=1482 YBD=1481 YBD=1490 YHG=1450>It YHG=1451>has YHG=1450>been YHG=1451>known YHG=1452>for YHG=1461>some YHG=1450>years "|>" XBD=1271 XBD=1322 XHG=1060 XHG=1284 YBD=1489 YBD=1481 YHG=1452>organisms " XBD=2582 XBD=1618 XHG=2488 XBD=1780 XBD=1911 XBD=2052 XBD=2181 XBD=2275 XBD=2456 XHG=1384 YBD=1492 XHG=1649 XHG=1811 XHG=1940 XHG=2083 XHG=2209 XHG=2308 YBD=1501 YHG=1452>of YBD=1497 YBD=1491 YHG=1472>appearance YHG=1462>noted, YHG=1462>films YHG=1461>made YHG=1461>from YHG=1463>the YHG=1462>oedema YHG=1462>fluid "" XBD=1324 XBD=209 XBD=487 XBD=641 XBD=957 XBD=1226 XHG=152 XHG=229 XHG=510 XHG=664 XHG=978 XHG=1246 YBD=1519 YBD=1528 YBD=1529 YBD=1529 YBD=1521 YHG=1489>the YHG=1498>gas-gangrene YHG=1500>group, YHG=1491>staphylococci, YHG=1491>streptococci YHG=1491>and XBD=1510 XHG=2504 XBD=1721 XBD=1822 XBD=2143 XBD=2460 XBD=2583 XHG=1388 YBD=1531 XHG=1553 XHG=1763 XHG=1863 XHG=2184 YBD=1539 YHG=1502>and YBD=1540 YBD=1538 YBD=1537 YHG=1503>(when YHG=1502>present) YHG=1501>for YHG=1501>microscopical YHG=1501>examination, " XBD=397 XBD=601 XBD=674 XBD=879 XBD=949 XBD=1078 XHG=153 XHG=426 XHG=632 XHG=703 XHG=978 XHG=1106 YBD=1567 YBD=1568 YBD=1561 YBD=1560 YBD=1567 YHG=1530>pneumococci YHG=1530>produce, YHG=1531>in YHG=1530>addition YHG=1531>to YHG=1530>their YHG=1530>recognised " XBD=1324 XBD=1550XHG=906 XBD=1617 XBD=1794 XBD=1927 XBD=2063 XBD=2215 XBD=2411 XBD=2580 XHG=1386 XHG=1578 XHG=1646 XHG=1823 XHG=1955 XHG=2091 XHG=2243 XHG=2437 YBD=1579 YBD=1570 YBD=1576 YBD=1578 YBD=1571 YHG=1542>samples YHG=1541>of YHG=1540>oedema YHG=1540>fluid, YHG=1541>heart YHG=1541>blood, YHG=1540>injected YHG=1535>muscle " XBD=1325 XBD=279 XHG=800 XBD=513 XBD=653 XBD=782 XBD=852 XBD=1085 XBD=1267 XHG=152 XHG=296 XHG=529 XHG=672 XHG=867YBD=1600 XHG=1102 XHG=1283 YBD=1604 YBD=1601 YBD=1600 YBD=1600 YHG=1568>toxins, YHG=1568>substances YHG=1571>which YHG=1579>cause YHG=1580>an YHG=1569>immediate YHG=1570>increase YHG=1570>in " XBD=2388 XBD=2585 XHG=2432 XBD=1463 XBD=1613 XBD=1841 XBD=1942 XBD=2259 XHG=1385 YBD=1610 XHG=1506 XHG=1660 XHG=1883 XHG=1987 XHG=2309 YBD=1611 YHG=1580>excised YBD=1609 YHG=1581>and YHG=1580>urine YHG=1580>collected YHG=1580>for YHG=1579>examination. YHG=1580>The " XBD=207 XBD=480 XBD=541 XBD=627 XBD=872 XHG=652 XHG=151 XHG=227 XHG=502 XHG=890 YBD=1638 YBD=1646 YBD=1639 YBD=1640 YBD=1640 YHG=1608>the YHG=1608>permeability YHG=1609>of YHG=1610>the YHG=1610>connective YHG=1609>tissues. " XBD=1040 XBD=1525XHG=560 XBD=1635 XBD=1815 XBD=1922 XBD=2152 XBD=2276 XBD=2329 XBD=2512 XBD=2583 XHG=1386 XHG=1556 XHG=1665 XHG=1845 XHG=1952 XHG=2180 XHG=2305 XHG=2361 XHG=2544 YBD=1651 YBD=1648 YBD=1650 YBD=1649 YHG=1620>muscle YHG=1629>was YHG=1620>minced YHG=1619>and YHG=1619>extracted YHG=1619>with YHG=1628>a YHG=1619>volume YHG=1619>of

" XBD=2583 XBD=1556 XBD=1690 XBD=1816 XBD=1875 XBD=1955 XBD=2112 XBD=2168 XBD=2369 XBD=2425 XHG=1386 XHG=1570 XHG=1705 XHG=1830 XHG=1890 XHG=1971 XHG=2126 XHG=2183 XHG=2386 XHG=2441 YBD=1690 YBD=1697 YBD=1689 YBD=1696 YBD=1688 YHG=1660>distilled YHG=1659>water YHG=1659>equal YHG=1659>to YHG=1658>the YHG=1659>weight YHG=1659>in YHG=1668>grammes YHG=1659>of YHG=1659>muscle XBD=384 XHG=695 XBD=500 XBD=663 XBD=859 XBD=1082 XBD=1203 XBD=1326 XHG=188 XHG=414 XHG=533 XHG=890YBD=1701 XHG=1113 XHG=1236 YBD=1692 YBD=1693 YBD=1703 YBD=1693YHG=1656>diffusing YHG=1663>Substances YHG=1664>with YHG=1665>similar YHG=1664>properties YHG=1664>have YHG=1665>been

" XBD=2585 XBD=1531 XBD=1667 XBD=1728 XBD=1815 XBD=1978 XBD=2152 XBD=2265 XBD=2369 XBD=2503 XHG=1383 XHG=1568 XHG=1690 XHG=1749 XHG=1838 XHG=2001 XHG=2171 XHG=2288 XHG=2390 XHG=2525 YBD=1734 YBD=1729 YBD=1728 YHG=1699>tissue YHG=1698>films YHG=1698>of YHG=1699>the YHG=1699>muscle YHG=1699>extract YHG=1708>were YHG=1698>also YHG=1698>made YHG=1698>for XBD=301 XHG=671 XBD=412 XBD=654 XBD=889 XBD=1060 XBD=1118 XBD=1325 XHG=151 XHG=324 XHG=428 XHG=907YBD=1739 XHG=1078 XHG=1131 YBD=1731 YBD=1732 YBD=1733 YBD=1733 YBD=1732 YBD=1740 YHG=1703>obtained YHG=1703>from YHG=1705>mammalian YHG=1704>testis, YHG=1707>extracts YHG=1703>of YHG=1704>malignant 9;13 1-4 6

and "|>"XBD=2584 XBD=1667 XBD=1980 XBD=2107 XBD=2307 XHG=1386 XHG=1704 XHG=2030 XHG=2146 XHG=2410 YBD=1776 YBD=1768 YBD=1775 YBD=1774 YHG=1738>microscopical YHG=1737>examination. YHG=1739>The YHG=1738>samples YHG=1736>oedema, XBD=288 XBD=319 XBD=454 XBD=557 XBD=703 XBD=900 XBD=1124 XBD=1325 XHG=152 XHG=307 XHG=348 XHG=485 XHG=586 XHG=733 XHG=1033 XHG=1163 YBD=1777 YBD=1761 YBD=1781 YBD=1772 YBD=1772 YBD=1780 YHG=1742>tissues, YHG=1742>4 YHG=1743>snake YHG=1744>and YHG=1744>spider YHG=1745>venoms YHG=1744>from YHG=1743>leevhes.1 " XBD=2584 XBD=1512 XBD=1685 XBD=1869 XBD=1977 XBD=2112 XBD=2237 XBD=2396 XBD=2486 XHG=1386 XHG=1544 XHG=1717 XHG=1898 XHG=2005 XHG=2142 XHG=2267 XHG=2425 XHG=2515 YBD=1814 YBD=1808 YBD=1807 YHG=1777>blood, YHG=1778>muscle YHG=1776>extract YHG=1777>and YHG=1777>urine YHG=1787>were YHG=1776>tested YHG=1777>for YHG=1777>the XBD=247 XHG=776 XBD=327 XBD=467 XBD=487 XBD=664 XBD=760 XBD=925 XBD=1104 XBD=1275 XBD=1329 XHG=150 XHG=258 XHG=338 XHG=474 XHG=500 XHG=680 XHG=941YBD=1821 XHG=1121 XHG=1289 YBD=1810 YBD=1811 YBD=1813 YBD=1803 YBD=1820 YBD=1812 YBD=1819 YBD=1812YHG=1783>purified YBD=1811 YHG=1781>Chain YHG=1782>and YHG=1783>Duthie YHG=1783>5 YHG=1783>reported YHG=1783>that YHG=1783>diffusing YHG=1785>extracts YHG=1782>of |>" XBD=1560 XBD=1623 XBD=1936 XBD=2035 XBD=2278 XBD=2343 XBD=2423 XBD=2586 XHG=1386 XHG=1584 XHG=1645 XHG=1958 XHG=2055 XHG=2301 XHG=2363 XHG=2445 YBD=1855 YBD=1848 YBD=1847 YBD=1854 YBD=1847 YHG=1826>presence YHG=1817>of YHG=1816>hyaluronidase YHG=1817>and YHG=1816>lecithinase YHG=1826>or YHG=1817>for YHG=1817>hsemo" XBD=312 XBD=395 XBD=577 XBD=704 XBD=854 XBD=892 XBD=1121 XBD=1324 XHG=150 XHG=326 XHG=410 XHG=593 XHG=719 XHG=870 XHG=907 XHG=1137 YBD=1851 YBD=1859 YBD=1852 YBD=1853 YBD=1858 YHG=1822>testicular YHG=1822>and YHG=1822>bacterial YHG=1823>origin YHG=1823>exhibit YHG=1833>a YHG=1823>remarkable YHG=1822>mucolytic XBD=1487XHG=884 XBD=1566 XBD=1712 XBD=1945 XBD=2022 XBD=2172 XBD=2423 XBD=2486 XBD=2586 XHG=1385 XHG=1523 XHG=1600 XHG=1740 XHG=1980 XHG=2048 XHG=2199 XHG=2452 XHG=2514 YBD=1894 YBD=1886 YBD=1889 YBD=1888 YBD=1887 YHG=1857>lysin YHG=1856>those YHG=1856>infections YHG=1857>in YHG=1857>which YHG=1850>lecithinase YHG=1858>is YHG=1857>not " XBD=1325 XBD=286 XBD=552 XBD=619 XBD=663 XBD=784 XBD=865 XBD=921 XBD=1001 XBD=1187 XBD=1247 XHG=150 XHG=302 XHG=571 XHG=640 XHG=681 XHG=803 XHG=941 XHG=1021 XHG=1207 XHG=1265 YBD=1898 YBD=1900 YBD=1891 YBD=1899 YBD=1892 YBD=1891 YBD=1899 YBD=1890 YHG=1861>activity YHG=1862>characterised YHG=1863>by YHG=1873>a YHG=1863>rapid YHG=1863>fall YHG=1864>in YHG=1863>the YHG=1863>viscosity YHG=1861>of YHG=1862>the " XHG=1385 YBD=1933 YHG=1896>produced. |>"XBD=1589 XBD=514 XBD=581 XBD=764 XBD=877 XBD=973 XBD=1151 XBD=1326 XBD=132XHG=901 XHG=150 XHG=541 XHG=603 XHG=789 XHG=997 XHG=1176 XHG=123YBD=1931 YBD=1938 YBD=1939 YBD=1931 YBD=1907YHG=1903>and YBD=1930 YHG=1901>mucopolysaccharide YHG=1902>of YHG=1903>synovial YHG=1903>fluid YHG=1902>vitreous YHG=1899>&dquo; YHG=1902>humour " XBD=1156 XBD=212 XBD=285 XBD=481 XBD=688 XBD=746 XBD=925 XHG=149 XHG=227 XHG=302 XHG=498 XHG=707 XHG=761 XHG=943 YBD=1969 YBD=1970 YBD=1979 YBD=1971 YHG=1940>and YHG=1941>the YHG=1941>liberation YHG=1941>therefrom YHG=1941>of YHG=1942>reducing YHG=1942>substances. XBD=1502 XHG=2398 XBD=1795 XBD=1959 XBD=2021 XBD=2105 XBD=2278 XBD=2373 XBD=2587 XHG=1429 YBD=1978 XHG=1526 XHG=1819 XHG=1983 XHG=2043 XHG=2130 XHG=2301 YBD=1977 YHG=1947>estimated YBD=1985 YBD=1986 YBD=1987 YBD=1979 YHG=1949>The YHG=1949>hyaluronidase YHG=1952>present YHG=1950>in YHG=1950>the YHG=1950>samples YHG=1959>was " XBD=1497 XBD=1581 XBD=1665 XBD=2048 XBD=2234 XBD=2343 XBD=2439 XBD=2587 XHG=1385 XHG=1532 XHG=1618 XHG=1702 XHG=2086 XHG=2271 XHG=2377 XHG=2476 YBD=2024 YBD=2017 YBD=2025 YBD=2026 YBD=2018 YHG=1989>either YHG=1989>by YHG=1989>its YHG=1988>viscosity-reducing YHG=1990>activity YHG=1989>and YHG=1990>the YHG=1990>result |>" " XBD=374 XBD=625 XBD=768 XBD=922 XBD=1033 XBD=1121 XBD=1326 XBD=674 XHG=152 XHG=396 XHG=698 XHG=792 XHG=943 XHG=1055 XHG=1148 XHG=644 YBD=2041 YBD=2033 YBD=2034 YBD=2033 YBD=2021 YBD=2032 YBD=2040 YHG=2002>Subsequent YHG=2003>work YHG=2004>has YHG=2003>shown YHG=2004>that YHG=2003>20 YHG=2003>the YHG=2003>diffusing 8 18 XBD=1572 XBD=1628 XBD=1992 XBD=2152 XHG=1385 XHG=1591 XHG=1644 XHG=2011 YBD=2064 YBD=2055 YHG=2027>expressed YHG=2028>in YHG=2028>viscosity-reducing YHG=2029>units 1919 " XBD=2279 XBD=2360 XHG=2231 XHG=2298 XHG=2380 YBD=2065 YBD=2057 YBD=2058 YHG=2029>by YHG=2029>the YHG=2029>mucin-clot " XBD=2586 XBD=188 XHG=793 XBD=401 XBD=564 XBD=650 XBD=768 XBD=930 XBD=1178 XBD=1232 XBD=1325 XHG=151 XHG=209 XHG=421 XHG=587 XHG=676 XHG=953YBD=2080 XHG=1202 XHG=1256 YBD=2081 YBD=2072 YBD=2078 YBD=2072 YHG=2051>or YHG=2042>spreading YHG=2042>factors YHG=2052>are YHG=2052>very YHG=2043>closely YHG=2042>associated, YHG=2042>if YHG=2042>not "" XBD=2584 XBD=1593 XBD=1682 XBD=1814 XBD=1986 XBD=2101 XBD=2178 XBD=2312 XBD=2370 XHG=1384 XHG=1611 XHG=1699 XHG=1832 XHG=2004 XHG=2117 XHG=2195 XHG=2331 XHG=2384 YBD=2104 YBD=2095 YBD=2097 YBD=2106 YBD=2096 YBD=2105 YHG=2067>prevention YHG=2070>test YHG=2068>which YHG=2068>depends YHG=2077>upon YHG=2069>the YHG=2078>power YHG=2068>of YHG=2066>hyaluroniXBD=329 XHG=725 XBD=447 XBD=493 XBD=636 XBD=703 XBD=900 XBD=1012 XBD=1235 XBD=1325 XHG=152 XHG=351 XHG=469 XHG=518 XHG=663 XHG=923YBD=2120 XHG=1035 XHG=1259 YBD=2118 YBD=2111 YBD=2119 YBD=2112 YBD=2112 YBD=2119 YBD=2111 YHG=2081>identical, YHG=2081>with YHG=2091>a YHG=2091>group YHG=2081>of YHG=2091>enzymes YHG=2081>that YHG=2081>hydrolyse YHG=2082>the " XBD=1468 XBD=1531 XBD=1698 XBD=1783 XBD=1968 XBD=2031 XBD=2112 XBD=2522 XBD=2584 XHG=1386 XHG=1492 XHG=1557 XHG=1722 XHG=1807 XHG=1993 XHG=2051 XHG=2135 XHG=2545 YBD=2134 YBD=2143 YBD=2136 YBD=2145 YBD=2135 YHG=2106>dase YHG=2108>to YHG=2106>destroy YHG=2107>the YHG=2108>capacity YHG=2108>of YHG=2108>the YHG=2107>mucopolysaccharide YHG=2110>to " XBD=551 XBD=714 XBD=778 XBD=1020 XBD=1129 XBD=1272 XBD=1327 XHG=152 XHG=574 XHG=735 XHG=800 XHG=1041 XHG=1149 XHG=1293 YBD=2159 YBD=2152 YBD=2151 YBD=2159 YBD=2151 YHG=2121>mucopolysaccharide YHG=2120>known YHG=2131>as YHG=2121>hyaluronic YHG=2121>acid YHG=2121>which YHG=2121>is " |>" XBD=1476 XBD=1517 XBD=1711 XBD=1843 XBD=1967 XBD=2034 XBD=2112 XBD=2290 XBD=2348 XBD=2487 XHG=1384 XHG=1496 XHG=1535 XHG=1724 XHG=1861 XHG=1988 XHG=2052 XHG=2131 XHG=2308 XHG=2363 YBD=2174 YBD=2183 YBD=2176 YBD=2175 YBD=2175 YHG=2146>form YHG=2155>a YHG=2146>typical YHG=2147>mucin YHG=2146>clot YHG=2156>on YHG=2147>the YHG=2147>addition YHG=2146>of YHG=2145>acetic &dquo; &dquo; " XBD=1326 XBD=275 XHG=661 XBD=513 XBD=567 XBD=645 XBD=879 XBD=1021 XBD=1137 XHG=152 XHG=287 XHG=525 XHG=577 XHG=891YBD=2191 XHG=1060 XHG=1151 YBD=2199 YBD=2190 YBD=2191 YBD=2190 YBD=2197 YHG=2161>widely YHG=2160>distributed YHG=2160>in YHG=2160>the YHG=2161>connective YHG=2160>tissue. YHG=2160>The YHG=2169>enzymes "" XBD=2585 XBD=1459 XBD=1616 XBD=1786 XBD=1845 XBD=1920 XBD=2083 XBD=2233 XBD=2362 XBD=2451 XHG=1386 XHG=1478 XHG=1636 XHG=1805 XHG=1859 XHG=1939 XHG=2102 XHG=2251 XHG=2381 XHG=2470 YBD=2213 YBD=2222 YBD=2224 YBD=2225 YBD=2216 YBD=2215 YHG=2186>The YHG=2185>highest YHG=2186>dilution YHG=2187>of YHG=2187>the YHG=2197>enzyme YHG=2187>sample YHG=2187>under YHG=2189>test YHG=2186>which XBD=208 XBD=400 XBD=578 XHG=152 XHG=222 XHG=418 YBD=2231 YBD=2238 YHG=2209>are YHG=2200>therefore YHG=2200>called YHG=2200>hyaluronidases.&dquo; " XBD=939 XBD=1456XHG=592 XBD=1619 XBD=1726 XBD=2038 XBD=2164 XBD=2404 XBD=2526 XHG=1385 XHG=1491 XHG=1656 XHG=1763 XHG=2073 XHG=2199 XHG=2438 XHG=2563 YBD=2256 YBD=2255 YBD=2254 YHG=2225>will &dquo; YHG=2225>inhibit YHG=2227>this YHG=2226>clot-formation YHG=2226>after YHG=2226>incubation YHG=2226>with YHG=2236>a " XBD=2584 XBD=232 XHG=955 XBD=310 XBD=417 XBD=561 XBD=662 XBD=742 XBD=941 XBD=993 XBD=1227 XBD=1327 XHG=196 XHG=243 XHG=323 XHG=430 XHG=572 XHG=674 XHG=755 XHG=1007 XHG=1240 YBD=2271 YBD=2270 YBD=2269 YBD=2276 YBD=2269 YHG=2240>It YHG=2241>has YHG=2240>been YHG=2240>shown YHG=2239>that YHG=2240>the YHG=2240>inclusion YHG=2239>of YHG=2238>hyaluronic YHG=2239>acid " XBD=1558 XBD=1756 XBD=1930 XBD=2007 XBD=2049 XBD=2165 XBD=2271 XBD=2323 XHG=1385 XHG=1579 XHG=1778 XHG=1951 XHG=2028 XHG=2072 XHG=2186 XHG=2293 XHG=2344 YBD=2299 YBD=2296 YBD=2295 YBD=2294 YBD=2293 YBD=2302 YHG=2265>standard YHG=2265>substrate YHG=2266>mixture YHG=2267>for YHG=2276>a YHG=2266>fixed YHG=2267>time YHG=2266>is YHG=2266>proportional " XBD=2586 XBD=188 XHG=950 XBD=269 XBD=433 XBD=624 XBD=687 XBD=766 XBD=928 XBD=992 XBD=1054 XBD=1326 XHG=152 XHG=206 XHG=293 XHG=456 XHG=648 XHG=712 XHG=790 XHG=1016 XHG=1077 YBD=2310 YBD=2309 YBD=2308 YBD=2307 YBD=2315 YHG=2279>in YHG=2280>the YHG=2279>culture YHG=2278>medium YHG=2279>of YHG=2278>Cl. YHG=2278>welchii YHG=2288>or YHG=2278>of YHG=2278>streptococci " XBD=1424 XBD=1506 XBD=1679 XBD=1739 XBD=1815 XBD=1980 XBD=2140 XBD=2217 XBD=2448 XBD=2508 XBD=2586 XHG=1386 XHG=1444 XHG=1525 XHG=1699 XHG=1755 XHG=1835 XHG=1999 XHG=2185 XHG=2238 XHG=2468 XHG=2524 YBD=2332 YBD=2342 YBD=2343 YBD=2341 YBD=2333 YHG=2294>to YHG=2304>the YHG=2306>potency YHG=2305>of YHG=2306>original YHG=2305>sample. YHG=2305>A YHG=2304>description YHG=2304>of YHG=2305>the " XBD=287 XBD=496 XBD=593 XBD=853 XBD=922 XBD=1241 XBD=1325 XHG=154 XHG=315 XHG=525 XHG=625 XHG=884 XHG=952 XHG=1273 YBD=2356 YBD=2349 YBD=2348 YBD=2355 YBD=2347 YBD=2355 YBD=2355 YHG=2319>greatly YHG=2319>increases YHG=2318>the YHG=2318>production YHG=2317>of YHG=2317>hyaluronidase " XBD=2585 XBD=1557 XHG=2382 XBD=1615 XBD=1773 XBD=2069 XBD=2214 XBD=2279 XHG=1386 YBD=2372 XHG=1575 XHG=1628 XHG=1791 XHG=2085 XHG=2232 XHG=2294 YBD=2373 YHG=2317>by YBD=2371 YHG=2344>influence YHG=2344>of YHG=2345>in-vitro YHG=2344>environmental YHG=2344>factors YHG=2353>on YHG=2343>this YHG=2344>manifesta" XBD=2366 XBD=635 XBD=754 XBD=873 XBD=1154 XBD=1262 XBD=1326 XHG=605 XHG=677 XHG=913 XHG=1192 XHG=1301 organisms,16 YBD=2375 YBD=2387 YBD=2386 YBD=2387 YBD=2386 17 YHG=2358>19 YHG=2357>and YHG=2357>this YHG=2356>observation YHG=2357>has YHG=2366>a XBD=248 XHG=793 XHG=153 YBD=2389 YHG=2358>these "" XBD=2590 XBD=1463 XBD=1521 XBD=1689 XBD=1855 XBD=1900 XBD=1954 XBD=2030 XBD=2184 XBD=2267 XBD=2305 XBD=2533 XHG=1385 XHG=1479 XHG=1532 XHG=1707 XHG=1871 XHG=1917 XHG=1969 XHG=2045 XHG=2201 XHG=2283 XHG=2322 XHG=2550 YBD=2419 YBD=2412 YBD=2420 YBD=2411 YHG=2384>tion YHG=2383>of YHG=2384>enzymic YHG=2384>activity YHG=2384>is YHG=2385>in YHG=2384>the YHG=2380>pTess,l8 YHG=2384>but YHG=2392>a YHG=2383>description YHG=2382>of XBD=295 XHG=737 XBD=373 XBD=466 XBD=705 XBD=776 XBD=1005 XBD=1088 XBD=1327 XHG=154 XHG=323 XHG=400 XHG=497 XHG=804YBD=2426 XHG=1034 XHG=1119 YBD=2434 YBD=2426 YBD=2433 YHG=2396>bearing YHG=2406>on YHG=2396>the YHG=2396>pathology YHG=2395>of YHG=2395>infections YHG=2396>by YHG=2396>organisms " XBD=1449 XBD=1608 XBD=1663 XBD=1885 XBD=1960 XBD=2045 XBD=2089 XBD=2206 XBD=2308 XBD=2359 XBD=2443 XHG=1387 XHG=1462 XHG=1623 XHG=1674 XHG=1900 XHG=1975 XHG=2059 XHG=2103 XHG=2220 XHG=2322 XHG=2372 XHG=2463 YBD=2452 YBD=2460 YBD=2451 YBD=2453 YBD=2450 YBD=2459 YHG=2422>the YHG=2423>method YHG=2423>of YHG=2423>performing YHG=2423>the YHG=2425>test YHG=2425>is YHG=2422>given YHG=2422>later YHG=2422>in YHG=2423>this YHG=2432>paper. " XBD=2584 XBD=233 XHG=771 XBD=405 XBD=525 XBD=729 XBD=843 XBD=1042 XBD=1325 XHG=153 XHG=247 XHG=419 XHG=542 XHG=858YBD=2465 XHG=1058 YBD=2474 YBD=2466 YBD=2473 YBD=2473 YBD=2472 YHG=2436>that YHG=2435>produce YHG=2436>these YHG=2444>enzymes. YHG=2436>For YHG=2435>example, YHG=2444>gas-gangrene " XBD=1505 XBD=1726 XBD=1899 XBD=1958 XBD=2034 XBD=2202 XBD=2312 XBD=2459 XBD=2585 XHG=1431 XHG=1525 XHG=1746 XHG=1919 XHG=1973 XHG=2054 XHG=2221 XHG=2330 XHG=2479 YBD=2491 YBD=2499 YBD=2498 YBD=2489 YBD=2492 YBD=2490 YHG=2462>The YHG=2462>lecithinase YHG=2465>potency YHG=2462>of YHG=2462>the YHG=2462>samples YHG=2460>from YHG=2462>welchii YHG=2462>infec" XBD=1326 XBD=296 XHG=793 XBD=471 XBD=668 XBD=763 XBD=957 XBD=1042 XBD=1167 XBD=1239 XHG=155 XHG=324 XHG=499 XHG=696 XHG=1000 XHG=1073 XHG=1196 XHG=1272 YBD=2513 YBD=2512 YBD=2504 YBD=2510 YBD=2505 YBD=2504 YBD=2506 YHG=2474>spreads YHG=2474>rapidly YHG=2475>through YHG=2475>the YHG=2474>tissues YHG=2484>soon ; YHG=2483>as YHG=2474>OZ. |>" " XBD=1464 XBD=1821 XBD=2064 XBD=2138 XBD=2225 XBD=2413 XBD=2587 XBD=1724 XHG=1386 XHG=1750 XHG=1848 XHG=2088 XHG=2164 XHG=2251 XHG=2438 XHG=1477 YBD=2530 YBD=2528 YBD=2536 YBD=2529 YBD=2538 YHG=2502>tion YHG=2511>was YHG=2501>determined YHG=2501>by YHG=2500>the YHG=2501>egg-yolk YHG=2501>method YHG=2502>’experiments " XBD=1327 XBD=281 XHG=794 XBD=415 XBD=477 XBD=714 XBD=776 XBD=861 XBD=1018 XHG=155 XHG=299 XHG=433 XHG=497 XHG=734 XHG=880YBD=2543 XHG=1038 YBD=2545 YBD=2544 YBD=2552 YBD=2544 YBD=2551 YHG=2514>welchii YHG=2514>starts YHG=2515>to YHG=2513>proliferate YHG=2514>in YHG=2514>the YHG=2513>tissues YHG=2514>hyaluronidase " XBD=1568 XBD=1637 XBD=1730 XBD=2001 XBD=2155 XBD=2236 XBD=2411 XHG=1388 XHG=1587 XHG=1658 XHG=1749 XHG=2042 XHG=2174 XHG=2256 XHG=2432 YBD=2569 YBD=2568 YBD=2567 YBD=2576 YBD=2577 YHG=2541>described YHG=2542>by YHG=2550>van YHG=2540>Heyningen.211 YHG=2539>When YHG=2539>the YHG=2540>enzymic YHG=2543>potency " XBD=2586 XBD=183 XHG=860 XBD=397 XBD=485 XBD=731 XBD=839 XBD=966 XBD=1022 XBD=1237 XBD=1326 XHG=154 XHG=203 XHG=419 XHG=509 XHG=753 XHG=988YBD=2583 XHG=1043 XHG=1260 YBD=2591 YBD=2582 YBD=2590 YBD=2583 YHG=2553>is YHG=2552>produced, YHG=2553>the YHG=2553>hyaluronic YHG=2552>acid YHG=2552>there YHG=2553>is YHG=2553>attacked, YHG=2553>the " XBD=1458 XBD=1560 XBD=1653 XBD=1709 XBD=1761 XBD=2009 XBD=2123 XBD=2189 XBD=2429 XHG=1388 XHG=1474 XHG=1576 XHG=1670 XHG=1725 XHG=1776 XHG=2023 XHG=2140 XHG=2208 XHG=2446 YBD=2615 YBD=2609 YBD=2616 YBD=2614 YBD=2607 YBD=2613 YBD=2608 YHG=2590>was YHG=2589>very YHG=2580>low, YHG=2589>as YHG=2580>in YHG=2580>experiments YHG=2578>using YHG=2579>CZ. YHG=2579>crdematiens, YHG=2580>estima" XBD=2586 XBD=403 XHG=766 XBD=456 XBD=540 XBD=698 XBD=749 XBD=857 XBD=1066 XBD=1156 XBD=1270 XBD=1328 XHG=156 XHG=418 XHG=473 XHG=558 XHG=715 XHG=875YBD=2622 XHG=1078 XHG=1168 XHG=1289 YBD=2630 YBD=2623 YBD=2623 YBD=2623 YHG=2592>permeability YHG=2593>of YHG=2593>the YHG=2593>tissues YHG=2593>is YHG=2592>thus YHG=2592>increased YHG=2593>and YHG=2601>more YHG=2593>of " XBD=1480 XBD=1580 XBD=1694 XBD=1805 XBD=1878 XBD=2073 XBD=2123 XBD=2356 XBD=2587 XHG=1387 XHG=1491 XHG=1591 XHG=1705 XHG=1817 XHG=1889 XHG=2085 XHG=2131 XHG=2366 YBD=2648 YBD=2654 YBD=2645 YBD=2646 YBD=2656 YHG=2620>tions YHG=2628>were YHG=2619>made YHG=2619>using YHG=2618>the YHG=2617>liberation YHG=2617>of YHG=2617>acid-soluble YHG=2618>phosphorus " XBD=212 XBD=417 XBD=533 XBD=725 XBD=944 XBD=1071 XBD=1267 XBD=1329 XHG=154 XHG=232 XHG=437 XHG=552 XHG=745 XHG=993 XHG=1092 XHG=1289 YBD=2662 YBD=2662 YBD=2670 YBD=2662 YHG=2632>the YHG=2632>substrate YHG=2631>then YHG=2633>becomes YHG=2631>available. YHG=2632>The YHG=2641>presence YHG=2632>of " XBD=1479 XBD=1665 XBD=1737 XBD=1948 XBD=2028 XBD=2270 XBD=2374 XBD=2586 XHG=1387 XHG=1511 XHG=1699 XHG=1771 XHG=1980 XHG=2060 XHG=2302 XHG=2405 YBD=2688 YBD=2686 YBD=2693 YBD=2685 YBD=2694 YHG=2660>from YHG=2658>lecithin, YHG=2667>as YHG=2656>described YHG=2657>by YHG=2657>Macfarlane YHG=2658>and YHG=2658>Knight.ll " XBD=360 XBD=457 XBD=600 XBD=679 XBD=876 XBD=1096 XBD=1151 XBD=1328 XHG=154 XHG=371 XHG=470 XHG=611 XHG=692 XHG=889 XHG=1107 XHG=1164 YBD=2701 YBD=2708 YBD=2709 YBD=2701 YBD=2709 YHG=2670>hyaluronic YHG=2671>acid YHG=2680>causes YHG=2671>the YHG=2671>infecting YHG=2671>organisms YHG=2671>to YHG=2672>produce " XBD=1564 XBD=1625 XBD=1884 XBD=2138 XBD=2207 XBD=2358 XBD=2479 XBD=2587 XHG=1388 XHG=1585 XHG=1641 XHG=1901 XHG=2159 XHG=2229 XHG=2376 XHG=2496 YBD=2728 YBD=2731 YBD=2725 YBD=2726 YHG=2696>Dilutions YHG=2697>of YHG=2696>standardised YHG=2696>glycerinated YHG=2696>Cl. YHG=2695>welchii YHG=2697>toxin YHG=2706>were " XBD=1328 XBD=200 XHG=916 XBD=400 XBD=578 XBD=636 XBD=721 XBD=897 XBD=994 XBD=1035 XBD=1200 XHG=155 XHG=216 XHG=418 XHG=597 XHG=654 XHG=741 XHG=1012 XHG=1055 XHG=1218 YBD=2741 YBD=2747 YBD=2740 YBD=2740 YBD=2741 YHG=2718>an YHG=2710>increased YHG=2711>amount YHG=2709>of YHG=2710>the YHG=2719>enzyme YHG=2711>and YHG=2720>a YHG=2711>vicious YHG=2711>circle " XBD=1539 XBD=1778 XBD=1994 XBD=2092 XBD=2286 XBD=2333 XBD=2366 XBD=2443 XBD=2549 XBD=2587 XHG=1388 XHG=1549 XHG=1790 XHG=2004 XHG=2102 XHG=2295 XHG=2346 XHG=2380 XHG=2453 XHG=2562 YBD=2766 YBD=2763 YBD=2772 YBD=2764 YBD=2748 YBD=2773 YBD=2765 YBD=2749 YHG=2737>usedfor YHG=2736>comparison, YHG=2735>and YHG=2744>were YHG=2736>expressed YHG=2745>as YHG=2736>&dquo; YHG=2745>egg YHG=2737>units YHG=2736>’&dquo; results " XBD=184 XBD=253 XBD=321 XBD=461 XBD=672 XBD=756 XBD=976 XBD=1032 XBD=1116 XBD=1328 XHG=154 XHG=199 XHG=271 XHG=339 XHG=479 XHG=689 XHG=775 XHG=994 XHG=1049 XHG=1132 YBD=2778 YBD=2786 YBD=2787 YBD=2779 YBD=2780 YBD=2780 YBD=2780 YHG=2748>is YHG=2749>set YHG=2758>up YHG=2748>which YHG=2749>promotes YHG=2750>the YHG=2749>extension YHG=2750>of YHG=2750>the YHG=2750>infection. " XBD=1503 XBD=1586 XBD=1666 XBD=1760 XBD=1806 XBD=2022 XBD=2077 XBD=2159 XBD=2294 XHG=1389 XHG=1520 XHG=1602 XHG=1682 XHG=1778 XHG=1823 XHG=2039 XHG=2094 XHG=2175 XHG=2309 YBD=2804 YBD=2811 YBD=2802 YBD=2803 YBD=2805 YHG=2776>where YHG=2785>one YHG=2776>egg YHG=2775>unit YHG=2775>is YHG=2774>equivalent YHG=2777>to YHG=2784>one YHG=2784>mouse YHG=2775>MLD. "" XBD=2419 XBD=263 XBD=539 XBD=612 XBD=718 XBD=891 XBD=962 XBD=1330 XHG=158 XHG=297 XHG=573 XHG=652 XHG=760 XHG=923 XHG=993 YBD=2825 YBD=2818 YBD=2819 YBD=2826 YHG=2787>Other YHG=2788>components YHG=2788>of YHG=2789>the YHG=2786>toxins YHG=2789>of YHG=2789>wound-infecting XBD=1480 XBD=1733 XBD=1840 XBD=1911 XBD=2095 XBD=2260 XBD=2453 XBD=2587 XHG=1434 XHG=1500 XHG=1753 XHG=1861 XHG=1933 XHG=2117 XHG=2279 XHG=2474 YBD=2843 YBD=2851 YBD=2842 YBD=2850 YBD=2844 YHG=2815>In YHG=2815>experiments YHG=2813>with YHG=2813>Cl. YHG=2814>septicum YHG=2815>(vibrion YHG=2816>septique), YHG=2815>which |>" " XBD=349 XBD=459 XBD=519 XBD=603 XBD=775 XBD=1273 XBD=1329 XBD=1045 XHG=156 XHG=367 XHG=476 XHG=537 XHG=623 XHG=1062 XHG=1290 XHG=793 YBD=2863 YBD=2857 YBD=2858 YBD=2859 YBD=2858 YBD=2866 YHG=2827>organisms YHG=2827>such YHG=2836>as YHG=2827>the YHG=2828>various YHG=2829>leucocidin YHG=2829>of YHG=2828>haemolysins, " XBD=1561 XBD=1639 XBD=1884 XBD=1977 XBD=2104 XBD=2237 XBD=2356 XBD=2504 XHG=1390 XHG=1593 XHG=1672 XHG=1916 XHG=2008 XHG=2134 XHG=2266 XHG=2387 XHG=2534 YBD=2890 YBD=2881 YBD=2886 YBD=2880 YBD=2888 YBD=2883 YBD=2882 YHG=2853>produces YHG=2862>no YHG=2852>lecithinase, YHG=2852>the YHG=2853>body YHG=2853>fluids YHG=2863>were YHG=2854>tested YHG=2853>for " XBD=2590 XBD=420 XHG=870 XBD=517 XBD=604 XBD=846 XBD=985 XBD=1146 XBD=1243 XBD=1329 XHG=155 XHG=440 XHG=529 XHG=624 XHG=1008 XHG=1165 XHG=1262 YBD=2903 YBD=2896 YBD=2897 YBD=2897 YBD=2898 YBD=2899 YHG=2865>staphylococci YHG=2867>and YHG=2866>-the YHG=2867>lecithinase YHG=2867>of’Cl. YHG=2867>ioelchii YHG=2868>and YHG=2867>the " XBD=1606 XBD=1768 XBD=1881 XBD=1996 XBD=2216 XBD=2279 XBD=2413 XBD=2590 XHG=1392 XHG=1657 XHG=1794 XHG=1906 XHG=2020 XHG=2243 XHG=2305 XHG=2437 YBD=2919 YBD=2920 YBD=2928 YBD=2923 YBD=2921 YHG=2892>hsemolysin. YHG=2891>These YHG=2893>tests YHG=2900>were YHG=2891>performed YHG=2893>in YHG=2893>saline YHG=2893>without |>" " XBD=256 XBD=313 XBD=721 XBD=822 XBD=1026 XBD=1088 XBD=1171 XBD=1331 XBD=389 XBD=639 XHG=155 XHG=276 XHG=658 XHG=742 XHG=840 XHG=1045 XHG=1105 XHG=1191 XHG=329 XHG=412 YBD=2934 YBD=2933 YBD=2936 YBD=2939 YBD=2935 YBD=2939 YBD=2936 YHG=2902>toxin YHG=2903>of YHG=2915>are YHG=2906>also YHG=2906>liberated YHG=2904>’OZ. YHG=2905>wdenzatiens YHG=2907>in YHG=2907>the YHG=2907>tissues " XBD=1514 XBD=1616 XBD=1851 XBD=2171 XBD=2265 XBD=2392 XBD=2442 XBD=2520 XBD=2590 XHG=1391 XHG=1529 XHG=1630 XHG=1864 XHG=2186 XHG=2277 XHG=2405 XHG=2457 XHG=2535 YBD=2965 YBD=2958 YBD=2960 YBD=2968 YBD=2959 YHG=2932>buffer, YHG=2931>with YHG=2929>McIlvaine’s YHG=2930>phosphate-citric YHG=2932>acid YHG=2932>buffer YHG=2934>at YHG=2932>pH YHG=2932>6-0 "" XBD=1275 XBD=225 XBD=337 XBD=489 XBD=597 XBD=664 XBD=884 XBD=954 XHG=157 XHG=242 XHG=355 XHG=506 XHG=616 XHG=683 XHG=901 XHG=972 YBD=2984 YBD=2972 YBD=2981 YBD=2974 YBD=2982 YBD=2983 YBD=2982 YHG=2943>and YHG=2942>their YHG=2944>spread YHG=2953>may YHG=2945>be YHG=2945>promoted YHG=2945>by YHG=2945>hyaluronidase. XBD=1464 XBD=1519 XBD=1598 XBD=1781 XBD=1840 XBD=2051 XHG=1393 XHG=1482 XHG=1538 XHG=1617 XHG=1800 XHG=1856 XHG=2073 YBD=2999 YBD=3006 YBD=2997 YBD=2998 YBD=3000 YHG=2970>and YHG=2970>in YHG=2970>the YHG=2978>presence YHG=2969>of YHG=2970>thiolacetic YHG=2971>acid. "|>"XBD=2160 XBD=516 XBD=731 XBD=1043 XBD=1156 XBD=1280 XBD=1333 XBD=417XHG=538 XHG=438 XHG=760 XHG=1066 XHG=1174 XHG=1299 XHG=179YBD=3020 YBD=3012 YBD=3014 YBD=3011YHG=2983>Knight YBD=3014 YBD=3013 YHG=2983>and YHG=2983>demonstrated YHG=2980>Macfarlane YHG=2983>that YHG=2984>there YHG=2984>is 11 XBD=203 XHG=788 XBD=364 XBD=422 XBD=492 XBD=649 XBD=775 XBD=912 XBD=1146 XBD=1331 XHG=157 XHG=216 XHG=380 XHG=439 XHG=510 XHG=662 XHG=926YBD=3052 XHG=1161 YBD=3048 YBD=3056 YBD=3050 YBD=3059 YHG=3027>an YHG=3029>enzyme YHG=3020>in YHG=3021>M. YHG=3021>welchii YHG=3022>toxin YHG=3022>hydrolyses YHG=3022>lecithin. " XBD=2592 XBD=1521 XBD=1645 XBD=1696 XBD=1774 XBD=1940 XBD=2124 XBD=2287 XBD=2338 XBD=2414 XHG=1443 XHG=1532 XHG=1658 XHG=1707 XHG=1787 XHG=1949 XHG=2135 XHG=2297 XHG=2347 XHG=2427 YBD=3052YHG=3022>which YBD=3061 YBD=3062 YBD=3053 YBD=3060 YHG=3024>The YHG=3022>titres YHG=3023>of YHG=3023>the YHG=3023>various YHG=3033>enzymes YHG=3024>present YHG=3024>in YHG=3024>the YHG=3023>samples " XBD=2592 XBD=241 XHG=919 XBD=480 XBD=635 XBD=759 XBD=893 XBD=960 XBD=1101 XBD=1205 XBD=1332 XBD=1493 XBD=1580 XBD=1770 XBD=1953 XBD=2068 XBD=2269 XBD=2367 XHG=158 XHG=263 XHG=508 XHG=660 XHG=784 XHG=986YBD=3091 XHG=1127 XHG=1229 XHG=1394 XHG=1513 XHG=1602 XHG=1790 XHG=1973 XHG=2090 XHG=2288 XHG=2389 YBD=3087 YBD=3089 YBD=3090 YBD=3090 YBD=3090YHG=3061>in YBD=3091 YBD=3094 YBD=3093 YBD=3092 YBD=3098 YHG=3056>This YHG=3059>lecithinase YHG=3061>differs YHG=3061>from YHG=3061>those YHG=3061>snake YHG=3061>and YHG=3069>wasp YHG=3062>from YHG=3061>the YHG=3061>infected YHG=3062>animals YHG=3073>were YHG=3063>recorded YHG=3062>and YHG=3062>compared XBD=302 XHG=851 XBD=364 XBD=451 XBD=632 XBD=695 XBD=832 XBD=885 XBD=1015 XBD=1103 XBD=1332 XHG=158 XHG=323 XHG=385 XHG=475 XHG=651 XHG=709 XHG=905YBD=3130 XHG=1036 XHG=1128 YBD=3126 YBD=3127 YBD=3128 YBD=3129 YBD=3137 YBD=3135 YHG=3104>venoms YHG=3098>in YHG=3098>the YHG=3108>manner YHG=3100>in YHG=3100>which YHG=3099>it YHG=3099>splits YHG=3100>the YHG=3099>substrate, " XBD=2591 XBD=1437 XBD=1541 XBD=1764 XBD=2030 XBD=2147 XBD=2411 XBD=2488 XHG=1377 XHG=1474 XHG=1577 XHG=1799 XHG=2081 XHG=2186 XHG=2448 XHG=2523 YBD=3130 YBD=3138 YBD=3131 YBD=3129 YHG=3108>as YHG=3100>infection YHG=3102>proceeded YHG=3102>the YHG=3101>correlation YHG=3100>of YHG=3101>the ; XBD=275 XHG=678 XBD=327 XBD=477 XBD=591 XBD=656 XBD=983 XBD=1213 XBD=1334 XHG=158 XHG=295 XHG=351 XHG=501 XHG=614 XHG=1006YBD=3175 XHG=1236 YBD=3165 YBD=3167 YBD=3174 YBD=3168 YBD=3175YHG=3138>immunological YHG=3135>whilst YHG=3135>it YHG=3136>differs YHG=3138>only YHG=3138>in YHG=3138>specificity YHG=3139>from " XBD=2592 XBD=1630 XBD=1698 XBD=1830 XBD=2032 XBD=2100 XBD=2190 XBD=2367 XBD=2487 XHG=1396 XHG=1659 XHG=1723 XHG=1858 XHG=2057 XHG=2123 XHG=2219 XHG=2394 XHG=2513 YBD=3176 YBD=3177 YBD=3169 YBD=3168 YHG=3148>appearance YHG=3139>of YHG=3140>these YHG=3149>enzymes YHG=3141>in YHG=3140>the YHG=3140>oedema YHG=3138>fluid YHG=3139>and |>|>" XBD=238 XBD=401 XBD=458 XBD=585 XBD=781 XBD=838 XBD=912 XBD=1333 XBD=1160 XHG=158 XHG=253 XHG=416 XHG=475 XHG=601 XHG=797 XHG=858 XHG=1175 XHG=933 YBD=3203 YBD=3211 YBD=3205 YBD=3206 YBD=3207 YBD=3207 YHG=3172>that YHG=3175>present YHG=3176>in YHG=3177>small YHG=3177>amounts YHG=3177>in YHG=3176>Cl. YHG=3177>toxin.10 YHG=3177>aedematiens " XBD=1534 XBD=1731 XBD=1849 XBD=1938 XBD=2136 XBD=2200 XBD=2436 XBD=2502 XBD=2591 XHG=1395 XHG=1560 XHG=1753 XHG=1872 XHG=1963 XHG=2163 XHG=2226 XHG=2459 XHG=2524 YBD=3209 YBD=3210 YBD=3215 YBD=3213 YBD=3206 YBD=3207 YHG=3177>muscle YHG=3177>extracts YHG=3179>with YHG=3179>the YHG=3187>presence YHG=3178>of YHG=3178>organisms YHG=3177>in YHG=3177>the " XBD=2287 XBD=1547 XBD=1666 XBD=1784 XBD=1910 XBD=2040 XBD=2138 XHG=1395 XHG=1567 XHG=1685 XHG=1803 XHG=1931 XHG=2058 XHG=2158 YBD=3246 YBD=3247 YBD=3245 YHG=3216>stained YHG=3216>films YHG=3216>from YHG=3217>these YHG=3217>fluids YHG=3226>was YHG=3215>noted. XBD=371 XHG=846 XBD=474 XBD=648 XBD=824 XBD=936 XBD=1025 XBD=1271 XBD=1335 XHG=159 XHG=395 XHG=499 XHG=672 XHG=959YBD=3245 XHG=1050 XHG=1297 YBD=3243 YBD=3244 YBD=3252 YBD=3246 YBD=3244 YBD=3244YHG=3215>that YHG=3210>Macfarlane YHG=3214>and YHG=3215>Knight YHG=3216>showed YHG=3215>the YHG=3215>lecithinase YHG=3214>of |>"XBD=2592 XBD=211 XBD=359 XBD=406 XBD=766 XBD=962 XBD=1072 XBD=1154 XBD=1196 XBD=1335 XBD=561XHG=783 XHG=162 XHG=227 XHG=373 XHG=583 XHG=977 XHG=1088 XHG=1174 XHG=1212 XHG=423YBD=3284 YBD=3281 YBD=3282 YBD=3290 YBD=3284 YBD=3282YHG=3253>identical YBD=3280 YHG=3249>Cl. YHG=3252>welchii YHG=3252>is YHG=3253>certainly YHG=3253>with YHG=3253>almost YHG=3254>the YHG=3261>a YHG=3253>toxin. " XBD=1637 XBD=1910 XBD=2024 XBD=2157 XBD=2270 XHG=1446 XHG=1657 XHG=1930 XHG=2046 XHG=2178 XHG=2294 YBD=3284 YBD=3292 YBD=3285 YBD=3283 YBD=3290 YHG=3254>Infection YHG=3256>experiments YHG=3264>were YHG=3255>made YHG=3254>with YHG=3254>representative

XBD=250 XBD=297 XBD=360 XBD=600 XBD=642 XBD=722 XBD=912 XBD=1016 XBD=1077 XBD=1205 XBD=1336 XHG=160 XHG=259 XHG=309 XHG=372 XHG=608 XHG=652 XHG=731 XHG=920 XHG=1025 XHG=1088 XHG=1213 YBD=3319 YBD=3320 YBD=3321 YBD=3322 YBD=3321 YHG=3288>That YHG=3290>of YHG=3290>Cl. YHG=3291>aedematiens YHG=3293>is YHG=3292>not YHG=3292>identical YHG=3292>with YHG=3292>its YHG=3291>lethal YHG=3291>toxin. |>" " XBD=1538 XBD=1595 XBD=1670 XBD=1839 XBD=1915 XBD=2107 XBD=2196 XBD=2402 XBD=2592 XHG=1397 XHG=1556 XHG=1616 XHG=1690 XHG=1861 XHG=1934 XHG=2129 XHG=2218 XHG=2419 YBD=3322 YBD=3323 YBD=3324 YBD=3329 YBD=3328 YBD=3321 YBD=3329 YHG=3292>strains YHG=3293>of YHG=3293>CL YHG=3294>welchli, YHG=3293>Cl. YHG=3293>septicum YHG=3292>(Cl. YHG=3292>aedematis YHG=3293>maligni) |>" " XBD=239 XBD=330 XBD=388 XBD=623 XBD=850 XBD=954 XBD=1076 XBD=1136 XBD=1205 XBD=1336 XBD=1475 XBD=1550 XBD=1903 XBD=2108 XBD=2151 XBD=2307 XBD=2402 XBD=2533 XBD=2593 XBD=1811 XHG=204 XHG=255 XHG=349 XHG=405 XHG=644 XHG=870 XHG=974 XHG=1094 XHG=1153 XHG=1221 XHG=1397 XHG=1496 XHG=1857 XHG=1920 XHG=2127 XHG=2170 XHG=2325 XHG=2421 XHG=2553 XHG=1572 YBD=3357 YBD=3359 YBD=3360 YBD=3366 YBD=3361 YBD=3365 YBD=3359 YBD=3366 YBD=3360 YBD=3362 YHG=3328>It YHG=3337>was YHG=3330>of YHG=3330>interest YHG=3330>determine YHG=3331>how YHG=3331>early YHG=3331>in YHG=3339>an YHG=3330>infecYHG=3330>and YHG=3330>Cl. YHG=3332>In YHG=3331>addition, YHG=3340>a YHG=3339>survey YHG=3339>was YHG=3331>made YHG=3331>of YHG=3331>aedematiens. to " XBD=1336 XBD=234 XBD=358 XBD=566 XBD=766 XBD=903 XBD=980 XBD=1182 XBD=1247 XHG=158 XHG=256 XHG=385 XHG=589 XHG=793 XHG=1007 XHG=1205 XHG=1270 YBD=3397 YBD=3405 YBD=3398 YBD=3398 YBD=3397 YHG=3364>tion YHG=3367>these YHG=3369>bacterial YHG=3376>enzymes YHG=3368>could YHG=3369>be YHG=3368>detected YHG=3368>in YHG=3367>the XBD=1539XHG=934 XBD=1597 XBD=1792 XBD=1954 XBD=2031 XBD=2150 XBD=2346 XBD=2408 XBD=2594 XHG=1396 XHG=1559 XHG=1616 XHG=1813 XHG=1973 XHG=2049 XHG=2171 XHG=2364 XHG=2427 YBD=3399 YBD=3403 YBD=3396 YBD=3404 YBD=3406 YHG=3368>strains YHG=3370>of YHG=3370>all, YHG=3370>species YHG=3369>for YHG=3368>their YHG=3368>capacity YHG=3370>to YHG=3370>produce three |>" " XBD=460 XBD=615 XBD=809 XBD=892 XBD=1160 XBD=1214 XBD=1336 XBD=402 XHG=419 XHG=476 XHG=631 XHG=825 XHG=911 XHG=1176 XHG=1230 XHG=155 YBD=3437 YBD=3438 YBD=3441 YBD=3436 YBD=3436 YBD=3437 YBD=3435 YHG=3417>or YHG=3407>wound YHG=3406>exudate, YHG=3407>the YHG=3406>distribution YHG=3406>YHG=3406>of YHG=3406>these cedema nuid XBD=1691 XBD=1757 XBD=1926 XBD=1993 XBD=2081 XBD=2280 XBD=2346 XBD=2595 XHG=1399 XHG=1716 XHG=1783 XHG=1950 XHG=2015 XHG=2107 XHG=2307 XHG=2370 YBD=3442 YBD=3444 YHG=3407>hyaluronidase YHG=3408>in YHG=3408>culture YHG=3407>in YHG=3406>the YHG=3415>presence YHG=3407>hyaluronic " XBD=2594 XBD=366 XHG=885 XBD=427 XBD=514 XBD=639 XBD=765 XBD=864 XBD=984 XBD=1230 XBD=1335 XBD=1497 XBD=1596 XBD=1696 XBD=1943 XBD=2055 XBD=2118 XBD=2214 XBD=2332 XBD=2523 XHG=160 XHG=387 XHG=448 XHG=537 XHG=654 XHG=787 XHG=1008 XHG=1241 XHG=1400 XHG=1550 XHG=1629 XHG=1728 XHG=1975 XHG=2085 XHG=2148 XHG=2248 XHG=2364 XHG=2555 YBD=3477 YBD=3476 YBD=3482 YBD=3475 YBD=3474 YBD=3474 YBD=3476 YBD=3482 YBD=3472 YBD=3475 YBD=3473 YHG=3445>substances YHG=3447>in YHG=3447>the YHG=3446>body YHG=3444>fluids YHG=3445>and YHG=3445>their YHG=3444>correlation YHG=3444>with YHG=3446>acid. YHG=3447>In YHG=3456>our YHG=3446>experience YHG=3443>this YHG=3443>is YHG=3444>the YHG=3444>best YHG=3445>method YHG=3445>of " XBD=2066 6238XHG=2034 YBD=3519 YHG=3496>M

of

or acid.

356

XBD=261 XHG=786 XBD=445 XBD=540 XBD=680 XBD=769 XBD=949 XBD=1046 XBD=1218 XBD=1278 XBD=1318 XHG=115 XHG=276 XHG=462 XHG=558 XHG=698 XHG=965YBD=235 XHG=1063 XHG=1236 XHG=1295 YBD=232 YBD=225 YBD=234 YBD=228 YBD=227 YBD=235YHG=197>produce YHG=192>testing YHG=194>whether YHG=205>any YHG=196>strain YHG=206>can YHG=197>this YHG=207>enzyme YHG=197>in YHG=207>a " XBD=2549 XBD=1455 XBD=1675 XBD=1777 XBD=1817 XBD=1919 XBD=1961 XBD=2097 XBD=2223 XBD=2441 XHG=1374 XHG=1476 XHG=1725 XHG=1797 XHG=1842 XHG=1940 XHG=1983 XHG=2124 XHG=2251 XHG=2460 YBD=236 YBD=227 YBD=228 YBD=229 YBD=234 YBD=230 YHG=198>also YHG=199>apparent. YHG=199>At YHG=199>2 YHG=199>and YHG=200>3 YHG=199>hours YHG=200>after YHG=199>infection, YHG=200>when |>" " XBD=588 XBD=705 XBD=793 XBD=1000 XBD=1066 XBD=1158 XBD=1319 XBD=281 XHG=306 XHG=638 XHG=730 XHG=818 XHG=1023 XHG=1092 XHG=1185 XHG=116 YBD=267 YBD=266 YBD=268 YBD=265 YHG=235>environment. YHG=235>We YHG=246>are YHG=236>indebted YHG=233>suitable YHG=235>to YHG=236>Dr. YHG=236>Muriel XBD=1481 XBD=1545 XBD=1623 XBD=1872 XBD=1986 XBD=2130 XBD=2258 XBD=2462 XBD=2552 XHG=1374 XHG=1511 XHG=1573 XHG=1660 XHG=1910 XHG=2026 XHG=2161 XHG=2294 XHG=2495 YBD=272 YBD=266 YBD=274 YBD=267 YHG=236>there YHG=236>is YHG=246>no YHG=237>detectable YHG=236>free YHG=237>fluid, YHG=237>both YHG=243>enzymes YHG=246>can XBD=1531XHG=752 XBD=1598 XBD=1797 XBD=1857 XBD=1942 XBD=2130 XBD=2305 XHG=1375 XHG=1552 XHG=1621 XHG=1815 XHG=1875 XHG=1962 XHG=2150 YBD=312 YHG=275>already YHG=275>be YHG=275>detected YHG=275>in YHG=275>the YHG=275>infected YHG=275>muscle. " XBD=1317 XBD=333 XBD=426 XBD=513 XBD=679 XBD=737 XBD=819 XBD=1087 XBD=1144 XHG=119 XHG=347 XHG=443 XHG=532 XHG=698 XHG=838 XHG=1105 XHG=1160 YBD=304 YBD=307 YBD=314 YBD=305 YBD=312 YBD=305 YBD=304 YHG=273>Robertson YHG=274>and YHG=276>Mr. YHG=276>Keppie YHG=277>of YHG=276>the YHG=274>Department YHG=275>of YHG=275>Animal "" "" "" " " " "" " " " " " " "

XBD=1315 XBD=344 XBD=601 XBD=693 XBD=758 XBD=881 XBD=943 XBD=1004 XBD=1128 XBD=1226 XHG=118 XHG=361 XHG=616 XHG=715 XHG=780 XHG=958 XHG=1021 XHG=1148 XHG=1250 YBD=351 YBD=352 YBD=344 YBD=343 YBD=343 YBD=343 YHG=312>Pathology, YHG=315>Cambridge, YHG=315>and YHG=315>to YHG=314>Prof. YHG=314>A. YHG=312>A. YHG=313>Miles YHG=313>and YHG=313>Dr. XBD=1492XHG=897 XBD=1630 XBD=1699 XBD=1891 XBD=2004 XBD=2055 XBD=2194 XBD=2303 XBD=2490 XBD=2552 XHG=1420 XHG=1522 XHG=1659 XHG=1724 XHG=1919 XHG=2034 XHG=2083 XHG=2222 XHG=2331 XHG=2513 YBD=362 YBD=361 YBD=360 YBD=367 YBD=359 YHG=333>The YHG=334>result YHG=333>of YHG=333>infection YHG=333>with YHG=343>a YHG=333>strain YHG=332>that YHG=331>produces YHG=341>no XBD=253 XBD=484 XBD=560 XBD=818 XBD=1000 XBD=1216 XBD=1316 XHG=120 XHG=288 XHG=520 XHG=596 XHG=855 XHG=1036 XHG=1256 YBD=390 YBD=392 YBD=384 YBD=389 YBD=388 YBD=388 YBD=381 YHG=352>Nancy YHG=352>Hayward YHG=354>of YHG=352>University YHG=352>College YHG=350>Hospital YHG=351>for XBD=1645 XBD=1800 XBD=1873 XBD=2052 XBD=2119 XBD=2241 XBD=2468 XBD=2553 XHG=1375 XHG=1674 XHG=1830 XHG=1902 XHG=2081 XHG=2149 XHG=2296 XHG=2493 YBD=408 YBD=401 YBD=400 YBD=399 YBD=405 YBD=397 YHG=372>hyaluronidase YHG=371>should YHG=372>be YHG=372>referred YHG=374>to YHG=371>here. YHG=369>Although YHG=369>this XBD=234 XBD=550 XBD=710 XBD=768 XBD=891 XBD=1132 XHG=117 XHG=251 XHG=569 XHG=729 XHG=784 XHG=911 YBD=429 YBD=430 YBD=422 YBD=421 YBD=427 YHG=400>many YHG=393>representative YHG=393>strains YHG=391>of YHG=391>these YHG=390>organisms. XBD=1486 XHG=2402 XBD=1574 XBD=1774 XBD=1946 XBD=2033 XBD=2226 XBD=2386 XBD=2551 XHG=1375 YBD=442 XHG=1502 XHG=1591 XHG=1794 XHG=1963 XHG=2048 XHG=2242 YBD=440 YHG=408>digestion YBD=447 YBD=439 YBD=438 YBD=446 YBD=436 YHG=412>strain YHG=420>was YHG=411>toxigenic, YHG=409>virulent YHG=410>and YHG=409>produced YHG=408>marked XBD=1415XHG=638 XBD=1572 XBD=1651 XBD=1807 XBD=1911 XBD=1997 XBD=2121 XBD=2234 XBD=2505 XBD=2553 XHG=1375 XHG=1428 XHG=1591 XHG=1672 XHG=1824 XHG=1927 XHG=2015 XHG=2138 XHG=2253 XHG=2520 YBD=484 YBD=478 YBD=477 YBD=476 YBD=475 YHG=450>of YHG=450>muscle, YHG=450>the YHG=451>oedema YHG=449>fluid YHG=458>was YHG=449>much YHG=457>more YHG=446>circumscribed YHG=448>to XBD=795 YBD=492 YHG=463>Results XBD=1435 XHG=2441 XBD=1612 XBD=1737 XBD=1844 XBD=1952 XBD=2043 XBD=2251 XBD=2418 XBD=2551 XHG=1374 YBD=522 XHG=1458 XHG=1633 XHG=1773 XHG=1865 XHG=1972 XHG=2066 XHG=2273 YBD=518 YHG=485>having YBD=523 YBD=517 YBD=524 YBD=520 YHG=490>the ; YHG=489>infected YHG=499>area YHG=488>this YHG=489>fluid YHG=497>was YHG=488>strikingly YHG=487>viscous, XBD=244 XHG=642 XBD=420 XBD=499 XBD=603 XBD=826 XBD=1117 XBD=1232 XBD=1314 XHG=167 XHG=282 XHG=460 XHG=515 XHG=865YBD=547 XHG=1153 XHG=1269 YBD=550 YBD=549 YBD=554 YBD=548YHG=516>infection YBD=547 YHG=520>The YHG=520>results YHG=519>of YHG=514>the YHG=517>experiments YHG=517>will YHG=517>be XBD=1434 XBD=1674 XBD=1734 XBD=1873 XBD=1966 XBD=2084 XBD=2164 XBD=2336 XBD=2477 XBD=2553 XHG=1373 XHG=1455 XHG=1694 XHG=1747 XHG=1895 XHG=1982 XHG=2103 XHG=2183 XHG=2354 XHG=2492 YBD=565 YBD=564 YBD=557 YBD=556 YBD=555 YBD=553 YBD=554 YHG=529>the YHG=529>consistency YHG=528>of YHG=529>honey, YHG=527>and YHG=527>when YHG=527>the YHG=526>infected YHG=525>muscle YHG=534>was XBD=376 XBD=468 XBD=702 XBD=830 XBD=1005 XBD=1190 XBD=1248 XBD=1314 XHG=119 XHG=397 XHG=492 XHG=853 XHG=1028 XHG=1213 XHG=1270 YBD=589 YBD=588 YBD=587 YBD=593 YBD=593 YBD=585 YBD=587 YHG=558>described’in YHG=558>full YHG=557>elsewhere. YHG=556>present YHG=565>purpose YHG=557>is YHG=556>to XBD=1523XHG=753 XBD=1570 XBD=1657 XBD=1785 XBD=1838 XBD=1898 XBD=2075 XBD=2178 XBD=2331 XBD=2440 XBD=2552 XHG=1373YBD=585 XHG=1541 XHG=1586 XHG=1672 XHG=1800 XHG=1856 XHG=1915 XHG=2091 XHG=2195 XHG=2347 XHG=2483 YBD=602YHG=556>Our YBD=598 YBD=596 YBD=604 YBD=595 YBD=594 YBD=593 YHG=568>minced, YHG=568>it YHG=577>was YHG=565>found YHG=570>to YHG=568>be YHG=563>dripping YHG=565>with YHG=566>viscous YHG=564>fluid. YHG=564>This XBD=202 XHG=856 XBD=288 XBD=429 XBD=687 XBD=838 XBD=954 XBD=1041 XBD=1316 XHG=117 XHG=221 XHG=309 XHG=450 XHG=708 XHG=974YBD=625 XHG=1061 YBD=635 YBD=627 YBD=624 YBD=632YHG=594>from YHG=598>give YHG=597>the YHG=597>broad YHG=594>conclusions YHG=594>drawn YHG=604>our YHG=595>experiments XBD=1542 XBD=1627 XBD=1710 XBD=1762 XBD=1979 XBD=2084 XBD=2193 XBD=2236 XBD=2321 XBD=2551 XHG=1373 XHG=1556 XHG=1643 XHG=1725 XHG=1777 XHG=1995 XHG=2101 XHG=2207 XHG=2250 XHG=2336 YBD=642 YBD=635 YBD=643 YBD=641 YBD=634 YBD=640 YHG=607>viscosity YHG=616>was YHG=607>due YHG=609>to YHG=606>hyaluronic YHG=606>acid, YHG=605>since YHG=606>it YHG=614>was YHG=603>immediately XBD=195 XHG=872 XBD=252 XBD=433 XBD=535 XBD=620 XBD=854 XBD=943 XBD=1004 XBD=1175 XBD=1232 XBD=1314 XHG=116 XHG=208 XHG=267 XHG=449 XHG=551 XHG=635 XHG=958YBD=664 XHG=1020 XHG=1188 XHG=1247 YBD=666 YBD=665 YBD=663 YBD=670 YBD=663 YBD=671 YBD=663 YHG=638>and YHG=636>to YHG=635>indicate YHG=635>how YHG=642>our YHG=634>experience YHG=643>can YHG=633>be YHG=634>applied YHG=634>to YHG=634>the XBD=1330 XBD=1562 XBD=1627 XBD=1705 XBD=1882 XBD=1939 XBD=2219 XBD=2328 XBD=2366 XBD=2506 XBD=2555 XHG=1327 XHG=1374 XHG=1579 XHG=1644 XHG=1724 XHG=1899 XHG=1952 XHG=2237 XHG=2345 XHG=2383 XHG=2522 YBD=677 YBD=674 YBD=682 YBD=672 YBD=673 YHG=671>, YHG=645>destroyed YHG=647>by YHG=646>the YHG=646>addition YHG=645>hyaluronidase YHG=644>from YHG=652>a YHG=643>culture YHG=644>of XBD=377 XHG=810 XBD=445 XBD=657 XBD=783 XBD=930 XBD=1048 XBD=1117 XBD=1330 XHG=116 XHG=405 XHG=472 XHG=683 XHG=959YBD=703 XHG=1075 XHG=1144 YBD=705 YBD=702 YBD=702 YBD=702 YBD=709 YHG=674>examination YHG=673>of YHG=672>exudates YHG=672>from YHG=673>either YHG=673>civil YHG=683>or YHG=671>military’ XBD=1522 XBD=1654 XBD=1715 XBD=1783 XBD=1945 XBD=2063 XBD=2188 XBD=2281 XBD=2420 XBD=2477 XBD=2552 XHG=1373 XHG=1543 XHG=1676 XHG=1733 XHG=1806 XHG=1990 XHG=2086 XHG=2210 XHG=2303 XHG=2441 XHG=2493 YBD=714 YBD=715 YBD=712 YBD=711 YBD=713 YHG=685>another YHG=685>strain YHG=684>of YHG=684>OZ. YHG=685>welchii. YHG=685>The YHG=693>cause YHG=684>and YHG=692>source YHG=683>of YHG=684>this

" " " " "

XBD=1314 XBD=258 XBD=466 XBD=744 XBD=845 XBD=922 XBD=1045 XBD=1152 XBD=1219 XHG=115 XHG=279 XHG=489 XHG=767 XHG=947 XHG=1070 XHG=1177 XHG=1241 YBD=782 YBD=788 YBD=780 YBD=781 YBD=780 YBD=786 YBD=780 YBD=787 YHG=752>animal YHG=751>infection YHG=750>experiments YHG=751>and YHG=760>we YHG=751>have YHG=751>not, YHG=759>so YHG=750>far, XBD=1444XHG=866 XBD=1497 XBD=1582 XBD=1743 XBD=1807 XBD=2090 XBD=2226 XBD=2318 XBD=2384 XBD=2551 XHG=1373 XHG=1468 XHG=1521 XHG=1606 XHG=1766 XHG=1826 XHG=2112 XHG=2250 XHG=2342 XHG=2408 YBD=792 YBD=793 YBD=791 YBD=800 YHG=765>and YHG=765>is YHG=764>the YHG=764>subject YHG=763>of YHG=763>investigations YHG=764>which YHG=763>will YHG=763>be YHG=764>reported XBD=193 XHG=690 XBD=292 XBD=580 XBD=656 XBD=910 XBD=1082 XBD=1314 XHG=115 XHG=225 XHG=329 XHG=616 XHG=943YBD=826 XHG=1116 YBD=827 YBD=820 YBD=820 YBD=821YHG=790>examining YHG=791>had YHG=791>the YHG=789>opportunity YHG=790>of YHG=787>wound YHG=790>exudates. XBD=1565 XHG=1372 YBD=832 YHG=803>elsewhere. XBD=206 XHG=845 XBD=355 XBD=407 XBD=542 XBD=765 XBD=825 XBD=911 XBD=1030 XBD=1093 XBD=1315 XHG=115 XHG=225 XHG=373 XHG=429 XHG=563 XHG=784 XHG=933YBD=858 XHG=1054 XHG=1115 YBD=866 YBD=867 YBD=859 YBD=866 YBD=859 YBD=866 YHG=829>This YHG=829>report YHG=828>is YHG=829>being YHG=828>published YHG=829>in YHG=829>the YHG=829>hope YHG=829>of YHG=828>obtaining

" XBD=284 XBD=397 XBD=502 XBD=718 XBD=826 XBD=959 XBD=1179 XHG=116 XHG=334 XHG=433 XHG=539 XHG=756 XHG=996 XHG=1214 YBD=744 YBD=742 YBD=742 YBD=741 YHG=713>wounds. YHG=712>All YHG=721>our YHG=711>evidence YHG=712>has YHG=711>obtained YHG=711>from " XBD=1314 XBD=1639XHG=864 XBD=1823 XBD=1881 XBD=2096 XBD=2195 XBD=2240 XBD=2298 XBD=2408 XBD=2550 XHG=1373YBD=741 XHG=1657 XHG=1841 XHG=1894 XHG=2116 XHG=2211 XHG=2259 XHG=2312 XHG=2425 YBD=762YHG=712>been YBD=753 YBD=751 YBD=760 YBD=752 YHG=725>extraordinary YHG=725>quantity YHG=724>of YHG=723>hyaluronic YHG=724>aoid YHG=724>is YHG=723>of YHG=725>great YHG=717>interest " XBD=2231 XBD=263 XBD=454 XBD=529 XBD=809 XBD=901 XBD=958 XBD=1082 XBD=1187 XBD=1313 XHG=114 XHG=278 XHG=469 XHG=545 XHG=823 XHG=973YBD=897 XHG=1098 XHG=1202 YBD=899 YBD=898 YBD=897 YBD=898 YHG=868>clinical YHG=867>material YHG=867>for YHG=868>examination YHG=868>and YHG=867>in YHG=867>order YHG=868>that YHG=868>those " XBD=2550XHG=916 XBD=1490 XBD=1735 XBD=1835 XBD=2008 XBD=2160 XHG=1371 XHG=1516 XHG=1758 XHG=1861 XHG=2033 XHG=2187 XHG=2256 YBD=900 YBD=901 YBD=902 YHG=880>TABLE YHG=880>I-HIGHEST YHG=879>AND YHG=879>LOWEST YHG=880>TITRES YHG=880>OF YHG=880>HYALURONIDASE " XBD=2550 XBD=1493 XHG=2273 XBD=1767 XBD=1991 XBD=2066 XBD=2240 XBD=2454 XHG=2484 YBD=941 XHG=1415 XHG=1526 XHG=1801 XHG=2024 XHG=2098 YBD=939 YHG=921>AND YBD=940 YHG=919>AND YHG=919>LECITHINASE YHG=919>DETECTED YHG=919>IN YHG=919>MUSCLE YHG=920>EXTRACTS

" XBD=1211 XBD=1312XHG=747 XBD=155 XBD=345 XBD=475 XBD=712 XBD=838 XBD=1099 XHG=113 XHG=190 XHG=378 XHG=511 XHG=872YBD=945 XHG=1133 XHG=1246 YBD=938 YBD=937 YBD=937 YBD=937 YHG=907>in YHG=906>contact YHG=907>with YHG=907>casualties YHG=916>may YHG=907>themselves YHG=907>test YHG=907>the " XBD=2549 XBD=347 XHG=446 XBD=416 XBD=515 XBD=817 XBD=1010 XBD=1082 XBD=1178 XBD=1312 XHG=545 XHG=113 XHG=377 XHG=846YBD=983 XHG=1039 XHG=1111 XHG=1209 YBD=984 YBD=976 YBD=983 YBD=975 YBD=976 YBD=983 YHG=945>application YHG=946>of YHG=955>our YHG=946>experimental YHG=945>findings YHG=945>to YHG=946>the YHG=946>early " XBD=1724 XBD=1808 XBD=2053 XBD=2247 XBD=2403 XHG=1592 XHG=1761 XHG=1845 XHG=2090 XHG=2286 XHG=2437 YBD=979 YBD=980 YBD=981 YHG=959>FLUIDS YHG=959>AT YHG=959>DIFFERENT YHG=959>PERIODS YHG=960>AFTER YHG=960>INTRA" XBD=1612 XBD=1832 XBD=1952 XBD=2023 XBD=2176 XHG=1415 XHG=1633 XHG=1853 XHG=1975 XHG=2047 YBD=1019 YBD=1018 YBD=1020 YHG=998>MUSCULAR YHG=998>INFECTION YHG=998>WITH YHG=990>Cl. YHG=990>welchii |>" XBD=305 XBD=366 XBD=527 XBD=743 XBD=1100 XHG=114 XHG=327 XHG=386 XHG=547 XHG=1097 YBD=1022 YBD=1015 YBD=1016 YBD=1022 YHG=985>diagnosis YHG=985>of YHG=985>wound YHG=985>infection. YHG=1018>,

ODEMA

" XBD=966 XBD=647 XHG=672 XHG=458 YBD=1092 YHG=1069>OBSERVATIONS YHG=1069>GENERAL

" XBD=1311 XBD=198 XHG=832 XBD=298 XBD=522 XBD=681 XBD=793 XBD=987 XBD=1065 XHG=162 XHG=233 XHG=337 XHG=559 XHG=716 XHG=1026 XHG=1102 YBD=1146 YBD=1153 YBD=1147 YBD=1154 YBD=1147 YBD=1155 YHG=1116>If YHG=1117>the YHG=1116>infecting YHG=1116>strain YHG=1126>was YHG=1116>capable YHG=1116>of YHG=1117>producing " XBD=1107 |>" XBD=416 XBD=667 XBD=768 XBD=850 XBD=1024 XBD=611 XBD=1309 XHG=113 XHG=635 XHG=691 XHG=795 XHG=875 XHG=1052 XHG=440 XHG=1132 YBD=1193 YBD=1185 YBD=1187 YBD=1186 YBD=1192 YBD=1193 YBD=1187 YBD=1194 YHG=1155>hyaluronidase, YHG=1155>it YHG=1166>was YHG=1156>Cl. YHG=1156>welchii, YHG=1155>whether YHG=1156>OZ. YHG=1156>septicum " XBD=1307 XBD=153 XHG=912 XBD=231 XBD=487 XBD=576 XBD=755 XBD=891 XBD=958 XBD=1158 XBD=1221 XHG=112 XHG=178 XHG=248 XHG=509 XHG=600 XHG=780 XHG=983YBD=1225 XHG=1179 XHG=1240 YBD=1230 YBD=1224 YBD=1232 YBD=1225 YBD=1225 YHG=1203>or YHG=1194>Cl. YHG=1194>cedematiens, YHG=1195>the YHG=1204>enzyme YHG=1195>could YHG=1196>be YHG=1196>detected YHG=1196>in YHG=1196>the " XBD=1307 XBD=258 XHG=762 XBD=379 XBD=449 XBD=572 XBD=641 XBD=734 XBD=948 XBD=1050 XHG=112 XHG=287 XHG=406 XHG=476 XHG=597 XHG=668 XHG=973YBD=1264 XHG=1078 YBD=1262 YBD=1263 YBD=1264 YBD=1272 YHG=1233>oedema YHG=1233>fluid YHG=1242>soon YHG=1242>as YHG=1234>the YHG=1234>infection YHG=1243>was YHG=1234>sufficiently " XBD=1308 XBD=305 XHG=861 XBD=368 XBD=554 XBD=726 XBD=840 XBD=920 XBD=1211 XHG=107 XHG=324 XHG=390 XHG=577 XHG=748 XHG=940YBD=1303 XHG=1262 YBD=1302 YBD=1310 YBD=1304 YBD=1303 YHG=1272>advanced YHG=1272>to YHG=1273>produce YHG=1273>enough YHG=1273>fluid YHG=1272>for YHG=1273>examination. YHG=1274>In " XBD=1306 XBD=290 XHG=811 XBD=573 XBD=697 XBD=781 XBD=960 XBD=1055 XHG=108 XHG=318 XHG=600 XHG=727 XHG=988YBD=1348 XHG=1084 YBD=1342 YBD=1349 YBD=1343 YBD=1342 YBD=1343 YHG=1311>infection YHG=1311>experiments YHG=1312>with YHG=1312>Cl. YHG=1312>welchii, YHG=1313>the YHG=1312>lecithinase " XBD=1304 XBD=220 XHG=894 XBD=279 XBD=474 XBD=528 XBD=607 XBD=880 XBD=987 XBD=1094 XBD=1216 XHG=110 XHG=235 XHG=297 XHG=486 XHG=540 XHG=625 XHG=1002 XHG=1117 XHG=1238 YBD=1380 YBD=1382 YBD=1381 YBD=1383 YBD=1389 YBD=1382 YHG=1350>could YHG=1350>be YHG=1350>detected YHG=1351>in YHG=1351>the YHG=1351>oedema YHG=1361>very YHG=1361>soon fluid YHG=1352>after YHG=1352>the " XBD=1304 XBD=290 XHG=871 XBD=381 XBD=618 XBD=691 XBD=857 XBD=914 XBD=1092 XBD=1186 XHG=106 XHG=304 XHG=398 XHG=633 XHG=707 XHG=929YBD=1422 XHG=1108 XHG=1200 YBD=1428 YBD=1421 YBD=1429 YBD=1429 YBD=1429YHG=1391>to YBD=1422 YHG=1389>infection YHG=1391>had YHG=1391>progressed YHG=1391>far YHG=1392>enough YHG=1391>produce YHG=1401>any YHG=1391>fluid. " XBD=1305 XBD=156 XHG=549 XBD=252 XBD=515 XBD=802 XBD=1064 XBD=1174 XHG=110 XHG=185 XHG=285 XHG=842YBD=1468 XHG=1096 XHG=1215 YBD=1460 YBD=1461 YBD=1461 YBD=1462 YBD=1468 YHG=1431>In YHG=1429>the YHG=1431>cedematiens YHG=1430>experiments YHG=1430>lecithinase YHG=1441>was YHG=1432>only " XBD=1304 XBD=320 XHG=842 XBD=385 XBD=521 XBD=755 XBD=820 XBD=908 XBD=1073 XBD=1139 XBD=1187 XHG=109 XHG=344 XHG=409 XHG=547 XHG=778 XHG=933YBD=1500 XHG=1097 XHG=1163 XHG=1212 YBD=1499 YBD=1507 YBD=1500 YBD=1500 YBD=1507 YHG=1469>detectable YHG=1471>small YHG=1470>quantities YHG=1470>in YHG=1470>the YHG=1471>muscle YHG=1471>at YHG=1480>a YHG=1480>very " XBD=1305 XBD=182 XHG=821 XBD=309 XBD=371 XBD=456 XBD=672 XBD=800 XBD=971 XBD=1101 XHG=105 XHG=201 XHG=330 XHG=389 XHG=475 XHG=723 XHG=992YBD=1546 XHG=1123 YBD=1545 YBD=1538 YBD=1540 YBD=1539 YBD=1539 YBD=1540 YHG=1508>late YHG=1509>stage YHG=1509>in YHG=1509>the YHG=1509>infection. YHG=1510>The YHG=1509>highest YHG=1509>value YHG=1510>obtained " XBD=1303 XBD=182 XHG=858 XBD=226 XBD=364 XBD=497 XBD=539 XBD=681 XBD=840 XBD=901 XBD=1084 XHG=105 XHG=205 XHG=246 XHG=383 XHG=515 XHG=559 XHG=701 XHG=921YBD=1578 XHG=1103 YBD=1577 YBD=1584 YBD=1585 YBD=1579 YBD=1586 YBD=1586 YHG=1556>was YHG=1548>5 YHG=1547>units, YHG=1548>using YHG=1558>a YHG=1548>strain YHG=1548>known YHG=1549>to YHG=1548>produce YHG=1548>relatively " XBD=1301 XBD=205 XHG=828 XBD=414 XBD=482 XBD=731 XBD=803 XBD=985 XBD=1116 XHG=105 XHG=234 XHG=442 XHG=508 XHG=759 XHG=1039 XHG=1144 YBD=1624 YBD=1617 YBD=1618 YBD=1618 YBD=1617 YHG=1587>large YHG=1587>amounts YHG=1588>of YHG=1588>lecithinase YHG=1588>in YHG=1587>culture. YHG=1588>The YHG=1587>relative

" " " "

XBD=1300XHG=810 XBD=393 XBD=452 XBD=646 XBD=706 XBD=789 XBD=949 XBD=1119 XBD=1215 XHG=107 XHG=413 XHG=470 XHG=665 XHG=723 XHG=969YBD=1657 XHG=1137 XHG=1233 YBD=1666 YBD=1657 YBD=1656YHG=1627>muscle YBD=1657 YHG=1626>concentration YHG=1626>of YHG=1636>enzymes YHG=1627>in YHG=1627>the YHG=1626>extract YHG=1627>and YHG=1627>the XBD=253 XHG=954 XBD=376 XBD=536 XBD=659 XBD=753 XBD=924 XBD=993 XBD=1086 XBD=1302 XHG=107 XHG=283 XHG=405 XHG=563 XHG=687 XHG=783 XHG=1020 XHG=1116 YBD=1697 YBD=1704 YBD=1696 YBD=1696 YBD=1704 YHG=1666>oedema YHG=1666>fluid YHG=1666>varied YHG=1667>with YHG=1666>the YHG=1666>species YHG=1666>of YHG=1666>the YHG=1666>infecting XBD=314 XHG=833 XBD=406 XBD=515 XBD=594 XBD=820 XBD=973 XBD=1026 XBD=1133 XBD=1300 XHG=106 XHG=327 XHG=418 XHG=527 XHG=608 XHG=988YBD=1736 XHG=1040 XHG=1146 YBD=1744 YBD=1737 YBD=1736 YBD=1736 YBD=1743 YHG=1706>organisms YHG=1706>and YHG=1706>with YHG=1706>the YHG=1705>individual YHG=1706>strains YHG=1706>of YHG=1706>each YHG=1706>species. XBD=184 XHG=741 XBD=359 XBD=542 XBD=619 XBD=725 XBD=779 XBD=1022 XBD=1077 XBD=1299 XHG=106 XHG=196 XHG=371 XHG=553 XHG=631 XHG=791YBD=1776 XHG=1038 XHG=1089 YBD=1777 YBD=1783 YBD=1776 YBD=1783 YHG=1745>The YHG=1745>relation YHG=1746>between YHG=1746>the YHG=1746>time YHG=1745>of YHG=1754>appearance YHG=1745>of YHG=1746>organisms

" XBD=1299 XBD=146 XHG=732 XBD=245 XBD=430 XBD=565 XBD=698 XBD=828 XBD=1002 XBD=1188 XHG=104 XHG=178 XHG=280 XHG=465 XHG=598 XHG=862YBD=1815 XHG=1036 XHG=1221 YBD=1816 YBD=1815 YBD=1815 YHG=1786>in YHG=1785>the YHG=1785>stained YHG=1785>films YHG=1785>from YHG=1784>both YHG=1785>muscle YHG=1785>extract YHG=1786>and

" XBD=1299 XBD=255 XBD=351 XBD=434 XBD=648 XBD=707 XBD=790 XBD=984 XBD=1044 XHG=107 XHG=273 XHG=367 XHG=456 XHG=667 XHG=724 XHG=1003 XHG=1060 XHG=1187 YBD=1855 YBD=1854 YBD=1862 YBD=1854 YHG=1824>oedema YHG=1825>and YHG=1824>detection YHG=1823>of YHG=1824>the YHG=1833>enzymes YHG=1824>in YHG=1824>these YHG=1824>fluids " XBD=1167 XBD=1664XHG=809 XBD=1775 XBD=1826 XBD=2031 XBD=2255 XBD=2366 XHG=1358 XHG=1691 XHG=1799 XHG=1846 XHG=2067 XHG=2292 XHG=2426 YBD=1879 YBD=1877 YBD=1871 YBD=1880 YBD=1876 YBD=1881 YHG=1850>Hyaluronidase.. YHG=1852>MCP YHG=1863>= YHG=1852>mucin-clot YHG=1852>prevention YHG=1852>test. YHG=1852>Figures " XBD=2543 XBD=184 XHG=725 XBD=337 XBD=452 XBD=539 XBD=704 XBD=803 XBD=945 XBD=1006 XBD=1092 XBD=1300 XHG=824 XHG=104 XHG=207 XHG=357 XHG=472 XHG=563 XHG=967YBD=1893 XHG=1026 XHG=1113 YBD=1894 YBD=1901 YBD=1894 YBD=1893 YBD=1901 YHG=1865>also YHG=1864>varied YHG=1864>with YHG=1864>the YHG=1864>species YHG=1863>and YHG=1863>strain YHG=1863>of YHG=1864>the YHG=1863>infecting " XBD=1875 XBD=2010 XBD=2166 XBD=2313 XBD=2355 XBD=2499 XHG=1736 XHG=1885 XHG=2021 XHG=2176 XHG=2321 XHG=2365 YBD=1907 YBD=1906 YBD=1910 YHG=1882>indicate YHG=1882>highest YHG=1881>effective YHG=1881>dilution YHG=1881>of YHG=1887>enzyme. XBD=1774 XBD=1825 XBD=2162 XHG=1685 XHG=1799 XHG=1845 XHG=2174 YBD=1936 YBD=1931 YBD=1939 YBD=1935 YHG=1912>VRU YHG=1922>= YHG=1911>viscosity-reducing YHG=1911>units. " XBD=2272 XBD=304 XHG=850 XBD=511 XBD=825 XBD=919 XBD=1162 XBD=1299 XHG=104 XHG=355 XHG=536 XHG=941YBD=1940 XHG=1188 YBD=1932 YBD=1941 YBD=1934 YBD=1933 YBD=1933 YHG=1904>organism. YHG=1904>Neither YHG=1903>hyaluronidase YHG=1912>nor YHG=1903>lecithinase YHG=1903>could " XBD=2543XHG=937 XBD=1663 XBD=1774 XBD=1824 XBD=1902 XBD=2025 XBD=2213 XBD=2379 XBD=2489 XHG=1356 XHG=1716 XHG=1799 XHG=1843 XHG=1928 XHG=2084 XHG=2240 XHG=2406 XHG=2513 YBD=1960 YBD=1969 YBD=1965 YHG=1940>Lecithinase.. YHG=1941>EU YHG=1951>= YHG=1947>egg YHG=1941>units. YHG=1941>Figures YHG=1940>indicate YHG=1941>units YHG=1942>of " XBD=150 XBD=349 XBD=410 XBD=495 XBD=624 XBD=688 XBD=786 XBD=914 XBD=976 XBD=1061 XBD=1297 XHG=105 XHG=174 XHG=368 XHG=428 XHG=517 XHG=646 XHG=709 XHG=807 XHG=994 XHG=1082 YBD=1973 YBD=1979 YBD=1972 YBD=1972 YBD=1972 YHG=1944>be YHG=1943>detected YHG=1943>in YHG=1942>the YHG=1943>urine YHG=1943>at YHG=1952>any YHG=1942>stage YHG=1943>of YHG=1942>the YHG=1942>infections. " XBD=2300 XBD=1867 XHG=2120 XBD=1943 XBD=2101 XBD=2154 XHG=2173 YBD=1995 XHG=1737 XHG=1887 XHG=1960 YBD=2000 YHG=1970>muscle. YBD=1999 YHG=1977>enzyme YHG=1976>per YHG=1976>gramme YHG=1970>of

" XBD=1299 XBD=259 XHG=906 XBD=445 XBD=554 XBD=672 XBD=878 XBD=948 XBD=1041 XHG=105 XHG=289 XHG=476 XHG=585 XHG=704 XHG=976YBD=2012 XHG=1074 YBD=2021 YBD=2013 YBD=2012 YBD=2019YHG=1982>in YHG=1982>Neither YHG=1992>enzyme YHG=1992>was YHG=1992>ever YHG=1981>detected YHG=1981>the YHG=1982>circulating " XBD=244 XBD=355 XBD=487 XBD=557 XBD=797 XBD=990 XBD=1049 XBD=1111 XBD=1213 XBD=1297 XHG=105 XHG=274 XHG=371 XHG=504 XHG=573 XHG=816 XHG=1007 XHG=1066 XHG=1128 XHG=1230 YBD=2058 YBD=2053 YBD=2052 YBD=2059 YBD=2052 YHG=2022>blood YHG=2022>this YHG=2023>raises YHG=2032>an YHG=2022>interesting YHG=2022>question YHG=2031>as YHG=2022>to YHG=2022>how YHG=2022>the ; " XBD=1559 XBD=1952 XBD=2177 XBD=2409 XHG=1505 XHG=1772 XHG=1969 XHG=2195 YBD=2071 YBD=2078 YBD=2077 YBD=2070 YHG=2040>Cl. YHG=2043>(VIBRION YHG=2042>SEPTIQUE) YHG=2047>INFECTION " XBD=1298 XBD=231 XHG=908 XBD=315 XBD=612 XBD=675 XBD=760 XBD=886 XBD=985 XBD=1038 XBD=1125 XHG=101 XHG=253 XHG=340 XHG=633 XHG=694 XHG=784 XHG=1005 XHG=1058 XHG=1148 YBD=2099 YBD=2092 YBD=2091 YBD=2099 YHG=2063>toxins YHG=2071>are YHG=2061>disseminated YHG=2062>in YHG=2062>the YHG=2062>body YHG=2062>and YHG=2062>is YHG=2061>the YHG=2061>subject " XBD=2540 XBD=1455 XBD=1553 XBD=1835 XBD=1959 XBD=2043 XBD=2250 XBD=2342 XHG=1408 XHG=1484 XHG=1582 XHG=1862 XHG=1989 XHG=2074 XHG=2275 XHG=2370 YBD=2131 YBD=2125 YBD=2132 YBD=2124 YHG=2094>In YHG=2103>our YHG=2095>experiments YHG=2095>with YHG=2094>Cl. YHG=2094>septicum YHG=2094>the YHG=2093>infection XBD=144 XHG=758 XBD=310 XBD=434 XBD=577 XBD=673 XBD=737 XBD=854 XBD=1068 XBD=1296 XHG=105 XHG=162 XHG=329 XHG=451 XHG=596 XHG=692 XHG=875YBD=2138 XHG=1086 YBD=2132 YBD=2131 YBD=2131 YHG=2102>of YHG=2102>further YHG=2101>work YHG=2101>which YHG=2102>will YHG=2102>be YHG=2100>fully YHG=2101>described YHG=2101>elsewhere. " XBD=2540 XBD=1568 XBD=1696 XBD=1855 XBD=1978 XBD=2099 XBD=2183 XBD=2359 XBD=2448 XHG=1359 XHG=1594 XHG=1724 XHG=1880 XHG=2004 XHG=2128 XHG=2210 XHG=2411 XHG=2471 YBD=2171 YBD=2163 YBD=2164 YHG=2133>developed YHG=2143>more YHG=2134>slowly YHG=2133>than YHG=2134>with YHG=2133>Cl. YHG=2133>welchii. YHG=2133>It YHG=2142>was XBD=139 XHG=871 XBD=387 XBD=439 XBD=516 XBD=667 XBD=861 XBD=913 XBD=1032 XBD=1298 XHG=105 XHG=152 XHG=400 XHG=449 XHG=528 XHG=680 XHG=922YBD=2170 XHG=1045 YBD=2179 YBD=2171 YBD=2171 YBD=2178YHG=2141>in YHG=2141>A YHG=2141>comparison YHG=2140>of YHG=2140>the YHG=2140>results YHG=2140>obtained YHG=2138>those YHG=2140>experiments " XBD=2541 XBD=1521 XBD=1591 XBD=1746 XBD=1851 XBD=2122 XBD=2245 XBD=2365 XBD=2414 XHG=1356 XHG=1548 XHG=1621 XHG=1774 XHG=1871 XHG=2142 XHG=2265 XHG=2393 XHG=2437 YBD=2203 YBD=2211 YBD=2210 YBD=2202 YHG=2173>unusual YHG=2173>to YHG=2172>detect YHG=2183>any YHG=2173>macroscopic YHG=2173>signs YHG=2173>until YHG=2172>6 YHG=2173>hours XBD=144 XHG=711 XBD=280 XBD=360 XBD=588 XBD=696 XBD=877 XBD=1063 XBD=1173 XBD=1298 XHG=103 XHG=156 XHG=293 XHG=376 XHG=601 XHG=889YBD=2218 XHG=1076 XHG=1188 YBD=2210 YBD=2217 YBD=2211 YBD=2218 YBD=2217 YHG=2181>in YHG=2181>which YHG=2180>the YHG=2180>organisms YHG=2189>were YHG=2179>injected YHG=2180>together YHG=2180>with YHG=2180>CaCl2 " XBD=2539 XBD=182 XHG=856 XBD=241 XBD=368 XBD=429 XBD=573 XBD=658 XBD=838 XBD=950 XBD=1131 XBD=1227 XBD=1297 XBD=1435 XBD=1610 XBD=1729 XBD=1830 XBD=1924 XBD=2098 XBD=2275 XBD=2341 XHG=104 XHG=200 XHG=258 XHG=388 XHG=447 XHG=590 XHG=678 XHG=972YBD=2250 XHG=1149 XHG=1247 XHG=1357 XHG=1462 XHG=1636 XHG=1752 XHG=1855 XHG=1949 XHG=2123 XHG=2299 XHG=2364 YBD=2249 YBD=2249 YBD=2249 YBD=2243 YBD=2250 YBD=2242 YBD=2244 YHG=2220>and YHG=2220>those YHG=2220>in YHG=2220>which YHG=2219>the YHG=2219>muscles YHG=2229>were YHG=2219>crushed YHG=2220>and YHG=2229>no YHG=2212>and YHG=2212>oedema YHG=2212>fluid YHG=2222>was YHG=2213>not YHG=2213>usually YHG=2212>present YHG=2213>in YHG=2212>sufficient " XBD=2540 XBD=1541 XBD=1620 XBD=1902 XBD=2022 XBD=2086 XBD=2222 XBD=2340 XHG=1360 XHG=1561 XHG=1640 XHG=1920 XHG=2043 XHG=2108 XHG=2241 XHG=2358 YBD=2289 YBD=2282 YBD=2283 YBD=2281 YHG=2252>quantity YHG=2251>for YHG=2252>examination YHG=2252>until YHG=2251>12 YHG=2253>hours YHG=2251>after YHG=2251>infection. XBD=263 XHG=869 XBD=463 XBD=606 XBD=835 XBD=958 XBD=1097 XBD=1210 XBD=1297 XHG=104 XHG=299 XHG=500 XHG=643 XHG=991YBD=2288 XHG=1132 XHG=1246 YBD=2295 YBD=2289 YBD=2289 YBD=2290 YHG=2259>calcium YHG=2259>chloride YHG=2259>used, YHG=2259>indicated YHG=2259>that YHG=2259>there YHG=2268>was YHG=2269>no " XBD=2540 XBD=1436 XBD=1542 XBD=1645 XBD=1704 XBD=1907 XBD=2001 XBD=2086 XBD=2376 XHG=1359 XHG=1458 XHG=1561 XHG=1665 XHG=1722 XHG=1924 XHG=2020 XHG=2107 XHG=2395 YBD=2328 YBD=2322 YBD=2321 YBD=2329 YHG=2292>The YHG=2291>first YHG=2291>sign YHG=2291>of YHG=2291>infection YHG=2301>was YHG=2291>the YHG=2291>haemorrhagic YHG=2300>appearXBD=318 XHG=694 XBD=535 XBD=595 XBD=677 XBD=857 XBD=1042 XBD=1123 XBD=1297 XHG=102 XHG=337 XHG=553 XHG=610 XHG=873YBD=2328 XHG=1057 XHG=1141 YBD=2337 YBD=2329 YBD=2336 YHG=2299>significant YHG=2298>difference YHG=2299>in YHG=2298>the YHG=2298>relation YHG=2298>between YHG=2298>the YHG=2308>appear" XBD=1450 XBD=1522 XBD=1615 XBD=1798 XBD=1902 XBD=1995 XBD=2209 XBD=2451 XBD=2537 XHG=1357 XHG=1482 XHG=1549 XHG=1645 XHG=1848 XHG=1929 XHG=2024 XHG=2237 XHG=2479 YBD=2361 YBD=2368 YBD=2360 YHG=2340>ance YHG=2330>of YHG=2331>muscle. YHG=2332>As YHG=2331>the YHG=2331>infection YHG=2330>proceeded, YHG=2330>the |>" XBD=194 XBD=255 XBD=340 XBD=636 XBD=721 XBD=848 XBD=909 XBD=993 XBD=1223 XBD=1298 XBD=537 XHG=101 XHG=216 XHG=273 XHG=558 XHG=654 XHG=741 XHG=871 XHG=927 XHG=1013 XHG=1256 XHG=361 YBD=2368 YBD=2374 YBD=2367 YBD=2375 YBD=2373 YHG=2347>ance YHG=2338>of YHG=2338>and YHG=2337>the YHG=2337>stage YHG=2337>of YHG=2338>the YHG=2346>enzymes YHG=2337>infection YHG=2338>in ; XBD=1541XHG=718 XBD=1597 XBD=1759 XBD=1868 XBD=2073 XBD=2235 XBD=2328 XBD=2406 XBD=2539 XHG=1360 XHG=1558 XHG=1613 XHG=1776 XHG=1881 XHG=2086 XHG=2250 XHG=2341 XHG=2422 YBD=2400 YBD=2407 YBD=2399 YHG=2369>quantity YHG=2369>of YHG=2370>oedema YHG=2369>fluid YHG=2370>increased YHG=2370>rapidly YHG=2370>and YHG=2370>the YHG=2368>distri" XBD=1296 XBD=171 XBD=344 XBD=571 XBD=677 XBD=876 XBD=1053 XHG=104 XHG=209 XHG=384 XHG=601 XHG=913 XHG=1091 YBD=2413 YBD=2409 YBD=2406 YBD=2407 YBD=2406 YBD=2414 YHG=2386>our YHG=2377>hands, YHG=2377>however, YHG=2376>the YHG=2377>crushed YHG=2377>muscle YHG=2376>technique " XBD=2536 XBD=1496 XBD=1557 XBD=1655 XBD=1769 XBD=2023 XBD=2108 XBD=2232 XBD=2330 XBD=2373 XHG=1358 XHG=1517 XHG=1575 XHG=1677 XHG=1788 XHG=2042 XHG=2130 XHG=2253 XHG=2349 XHG=2393 YBD=2440YHG=2416>rate YBD=2445 YBD=2446 YBD=2439 YBD=2438 YHG=2409>bution YHG=2409>of YHG=2408>this YHG=2408>fluid YHG=2408>throughout YHG=2409>the YHG=2409>body YHG=2418>was YHG=2418>a YHG=2406>marked XBD=268 XHG=885 XBD=340 XBD=444 XBD=685 XBD=758 XBD=854 XBD=968 XBD=1042 XBD=1197 XBD=1296 XHG=101 XHG=298 XHG=370 XHG=474 XHG=716 XHG=787 XHG=1001 XHG=1074 XHG=1229 YBD=2453 YBD=2445 YBD=2446 YHG=2416>resulted YHG=2415>in YHG=2415>less YHG=2415>regularity YHG=2416>in YHG=2416>the YHG=2415>at YHG=2416>which YHG=2417>the " XBD=656 XBD=1516XHG=540 XBD=1683 XBD=1775 XBD=1813 XBD=1937 XBD=2113 XBD=2170 XBD=2249 XBD=2536 XBD=282 XBD=527 XHG=99 XHG=296 XHG=1356 XHG=1567 XHG=1699 XHG=1789 XHG=1826 XHG=1950 XHG=2128 XHG=2181 XHG=2267 YBD=2485 YBD=2486 YBD=2486 YBD=2478 YBD=2479 YHG=2454>infection YHG=2454>established YHG=2454>itself. YHG=2449>feature. YHG=2448>There YHG=2457>was YHG=2457>a YHG=2448>rapid YHG=2448>increase YHG=2449>in YHG=2449>the YHG=2447>concentration " XBD=2535 XBD=1396 XHG=2457 XBD=1704 XBD=1761 XBD=1842 XBD=2008 XBD=2118 XBD=2336 XBD=2443 XHG=1358 YBD=2517 XHG=1413 XHG=1720 XHG=1776 XHG=1862 XHG=2026 XHG=2135 XHG=2350 YBD=2516 YHG=2486>that YBD=2525 YBD=2518 YHG=2488>of YHG=2487>hyaluronidase YHG=2487>in YHG=2487>the YHG=2487>oedema YHG=2488>fluid YHG=2488>compared YHG=2487>with XBD=1397 XBD=1480 XBD=1653 XBD=1795 XBD=2000 XBD=2061 XBD=2125 XBD=2353 XBD=2461 XBD=2533 XHG=1355 XHG=1413 XHG=1501 XHG=1704 XHG=1812 XHG=2018 XHG=2081 XHG=2147 XHG=2369 XHG=2476 YBD=2557 YBD=2558 YBD=2556 YHG=2528>in YHG=2528>the YHG=2527>muscle. YHG=2526>This YHG=2527>appeared YHG=2528>to YHG=2528>be YHG=2527>correlated YHG=2527>with YHG=2526>the |>" " XBD=536 XBD=921 XBD=692 XHG=482 XHG=707 XHG=554 YBD=2565 YBD=2564 YBD=2564 YHG=2533>Cl. YHG=2540>INFECTION YHG=2533>welchii "" XBD=2533 XBD=1458 XBD=1715 XBD=1779 XBD=2010 XBD=2073 XBD=2160 XBD=2333 XBD=2446 XHG=1355 XHG=1481 XHG=1740 XHG=1802 XHG=2032 XHG=2094 XHG=2187 XHG=2355 XHG=2466 YBD=2604 YBD=2605 YBD=2595 YBD=2603 YBD=2596 YBD=2597 YHG=2568>early YHG=2574>appearance YHG=2565>of YHG=2566>organisms YHG=2567>in YHG=2567>the YHG=2567>oedema YHG=2566>fluid YHG=2564>and XBD=198 XBD=281 XBD=453 XBD=741 XBD=839 XBD=1056 XBD=1296 XHG=152 XHG=230 XHG=315 XHG=486 XHG=772 XHG=872 XHG=1091 YBD=2618 YBD=2625 YBD=2617 YBD=2618 YBD=2624 YHG=2587>In YHG=2588>all YHG=2587>avelchii YHG=2588>experiments YHG=2587>the YHG=2587>infection YHG=2588>developed " XBD=1454 XBD=1591 XBD=1780 XBD=1849 XBD=2050 XBD=2200 XBD=2411 XBD=2531 XHG=1354 XHG=1480 XHG=1616 XHG=1807 XHG=1874 XHG=2077 XHG=2226 XHG=2434 YBD=2643 YBD=2634 YBD=2636 YBD=2635 YHG=2606>their YHG=2606>rapid YHG=2606>increase YHG=2605>in YHG=2606>numbers YHG=2606>which YHG=2605>exceeded YHG=2606>those " XBD=260 XBD=429 XBD=541 XBD=599 XBD=799 XBD=1003 XBD=1079 XBD=1296 XHG=99 XHG=282 XHG=448 XHG=558 XHG=619 XHG=816 XHG=1020 XHG=1098 YBD=2663 YBD=2656 YBD=2664 YBD=2656 YBD=2657 YHG=2626>rapidly, YHG=2626>oedema YHG=2626>fluid YHG=2627>in YHG=2627>quantity YHG=2627>sufficient YHG=2627>for " XBD=2259 XBD=1443 XHG=2078 XBD=1501 XBD=1585 XBD=1699 XBD=1816 XBD=1899 XBD=2058 XHG=1355 YBD=2675 XHG=1461 XHG=1520 XHG=1608 XHG=1717 XHG=1833 XHG=1919 YBD=2673 YHG=2626>collection YBD=2674 YHG=2655>seen YHG=2645>in YHG=2644>fluid YHG=2644>from YHG=2645>the YHG=2645>muscle YHG=2645>extracts. " XBD=178 XBD=352 XBD=529 XBD=653 XBD=700 XBD=853 XBD=907 XBD=1123 XBD=1295 XHG=100 XHG=202 XHG=377 XHG=554 XHG=678 XHG=726 XHG=874 XHG=931 XHG=1145 YBD=2702 YBD=2703 YBD=2696 YBD=2701 YBD=2695 YBD=2696 YBD=2704 YHG=2674>was YHG=2665>usually YHG=2666>present YHG=2666>after YHG=2667>4 YHG=2666>hours, YHG=2666>it YHG=2666>increased YHG=2666>rapidly " XBD=1453 XBD=1616 XBD=1693 XBD=1889 XBD=2090 XBD=2223 XBD=2314 XBD=2532 XHG=1406 XHG=1472 XHG=1639 XHG=1715 XHG=1906 XHG=2108 XHG=2240 XHG=2331 YBD=2715 YBD=2720 YBD=2714 YBD=2721 YHG=2685>In YHG=2685>culture YHG=2683>Cl. YHG=2683>septicum YHG=2684>produces YHG=2685>much YHG=2684>less YHG=2682>hyaluroni" XBD=178 XBD=366 XBD=636 XBD=905 XBD=1007 XBD=1160 XBD=1295 XHG=99 XHG=214 XHG=405 XHG=671 XHG=941 XHG=1046 XHG=1217 YBD=2734 YBD=2734 YBD=2741 YBD=2742 YBD=2734 YBD=2742 YBD=2735 YHG=2704>and YHG=2704>became YHG=2705>generalised YHG=2705>throughout YHG=2704>the YHG=2705>body. YHG=2705>The " XBD=1444 XBD=1560 XBD=1630 XBD=1785 XBD=1887 XBD=2101 XBD=2149 XBD=2240 XBD=2325 XBD=2532 XHG=1356 XHG=1460 XHG=1577 XHG=1647 XHG=1798 XHG=1902 XHG=2115 XHG=2163 XHG=2255 XHG=2339 YBD=2758 YBD=2759 YBD=2754 YBD=2753 YBD=2761 YHG=2723>dase YHG=2724>than YHG=2723>Cl. YHG=2722>weZchii YHG=2723>and, YHG=2723>therefore, YHG=2723>it YHG=2732>was YHG=2723>not YHG=2723>surprising " XBD=308 XBD=523 XBD=588 XBD=678 XBD=853 XBD=970 XBD=1192 XBD=1294 XHG=100 XHG=333 XHG=547 XHG=611 XHG=705 XHG=878 XHG=993 XHG=1217 YBD=2780 YBD=2775 YBD=2774 YBD=2781 YBD=2774 YHG=2743>organisms YHG=2744>appeared YHG=2744>in YHG=2745>the YHG=2744>cedema YHG=2744>fluid YHG=2744>relatively YHG=2744>late " XBD=1397 XBD=1501 XBD=1612 XBD=1676 XBD=1762 XBD=1889 XBD=2024 XBD=2086 XBD=2174 XBD=2364 XBD=2534 XHG=1354 XHG=1420 XHG=1522 XHG=1634 XHG=1696 XHG=1787 XHG=1913 XHG=2048 XHG=2107 XHG=2197 XHG=2384 YBD=2799 YBD=2793 YBD=2792 YHG=2763>to YHG=2763>find YHG=2763>that YHG=2763>in YHG=2762>body YHG=2762>fluids YHG=2762>of YHG=2763>the YHG=2762>infected YHG=2762>animals " XBD=141 XBD=232 XBD=456 XBD=583 XBD=676 XBD=878 XBD=1017 XBD=1089 XBD=1295 XHG=99 XHG=166 XHG=260 XHG=483 XHG=609 XHG=704 XHG=907 XHG=1043 XHG=1119 YBD=2812 YBD=2817 YBD=2812 YBD=2820 YBD=2813 YBD=2813 YHG=2783>in YHG=2782>the YHG=2782>infection, YHG=2783>after YHG=2783>the YHG=2793>enzvmes YHG=2783>could YHG=2784>be YHG=2783>detected " XBD=1419 XBD=1604 XBD=1662 XBD=1758 XBD=1930 XBD=2026 XBD=2304 XBD=2430 XHG=1353 XHG=1438 XHG=1623 XHG=1678 XHG=1774 XHG=1949 XHG=2044 XHG=2320 XHG=2446 YBD=2838 YBD=2839 YBD=2832 YBD=2831 YHG=2802>the YHG=2802>potency YHG=2802>of YHG=2802>this YHG=2810>enzyme YHG=2810>was YHG=2801>considerably YHG=2802>lower YHG=2801>than "" XBD=2531 XBD=178 XBD=463 XBD=584 XBD=706 XBD=821 XBD=958 XBD=1026 XBD=1140 XBD=1204 XBD=1293 XHG=100 XHG=202 XHG=486 XHG=607 XHG=728 XHG=848 XHG=981 XHG=1052 XHG=1162 XHG=1225 YBD=2851 YBD=2858 YBD=2852 YBD=2852 YBD=2852 YHG=2821>and YHG=2821>considerably YHG=2822>later YHG=2822>than YHG=2814>they YHG=2823>could YHG=2822>be YHG=2831>seen YHG=2821>in YHG=2822>the XBD=1395 XBD=1485 XBD=1650 XBD=1941 XBD=2031 XBD=2180 XBD=2467 XBD=2528 XHG=1353 XHG=1417 XHG=1513 XHG=1674 XHG=1992 XHG=2056 XHG=2205 XHG=2490 YBD=2870 YBD=2876 YBD=2877 YBD=2869 YHG=2841>in YHG=2840>the YHG=2841>welchii YHG=2839>experiments. YHG=2840>It YHG=2840>could, YHG=2840>nevertheless, YHG=2839>be " XBD=1294 XBD=198 XHG=866 XBD=333 XBD=432 XBD=623 XBD=765 XBD=832 XBD=906 XBD=1112 XBD=1189 XHG=100 XHG=232 XHG=366 XHG=469 XHG=674 XHG=798 XHG=943YBD=2890 XHG=1145 XHG=1225 YBD=2891 YBD=2889 YBD=2891 YBD=2890 YHG=2861>films YHG=2860>from YHG=2860>the YHG=2860>muscle. YHG=2861>This YHG=2862>is YHG=2861>in YHG=2861>contrast YHG=2861>to YHG=2870>our " XBD=1532 XBD=1648 XBD=1722 XBD=1808 XBD=2051 XBD=2298 XBD=2444 XBD=2530 XHG=1356 XHG=1553 XHG=1670 XHG=1741 XHG=1830 XHG=2072 XHG=2321 XHG=2463 YBD=2917 YBD=2908 YBD=2916 YBD=2909 YHG=2879>detected YHG=2880>both YHG=2880>by YHG=2879>the YHG=2878>mucin-clot YHG=2879>prevention YHG=2879>(MCP) YHG=2878>test "|>" XBD=317 XBD=501 XBD=695 XBD=786 XBD=1041 XBD=1199 XBD=1294 XBD=429 XHG=99 XHG=447 XHG=520 XHG=709 XHG=804 XHG=1083 XHG=1215 XHG=333 YBD=2937 YBD=2936 YBD=2929 YBD=2930 YBD=2929 YBD=2930 YHG=2892>experience YHG=2898>Cl. YHG=2899>septicum YHG=2900>and YHG=2900>cedematiens. YHG=2899>with YHG=2900>There YHG=2909>was " XBD=1431 XBD=1505 XBD=1792 XBD=1912 XBD=2052 XBD=2097 XBD=2233 XBD=2296 XBD=2381 XHG=1352 XHG=1453 XHG=1529 XHG=1813 XHG=1933 XHG=2076 XHG=2119 XHG=2254 XHG=2315 XHG=2403 YBD=2954 YBD=2955 YBD=2946 YBD=2947 YBD=2948 YHG=2918>and YHG=2918>by YHG=2918>viscosimetry YHG=2917>from YHG=2917>about YHG=2918>6 YHG=2918>hours YHG=2918>in YHG=2918>the YHG=2918>muscle " XBD=2530 XBD=354 XHG=793 XBD=573 XBD=769 XBD=860 XBD=1062 XBD=1228 XBD=1294 XHG=100 XHG=382 XHG=599 XHG=888YBD=2968 XHG=1088 XHG=1255 YBD=2969 YBD=2967 YBD=2969 YBD=2968 YHG=2938>considerable YHG=2938>variation YHG=2938>between YHG=2939>the YHG=2938>different YHG=2938>strains YHG=2939>of "|>" XBD=1430 XBD=1575 XBD=1644 XBD=1788 XBD=1850 XBD=1939 XBD=2243 XBD=2527 XBD=2473 XHG=1352 XHG=1455 XHG=1603 XHG=1671 XHG=1808 XHG=1873 XHG=1967 XHG=2488 XHG=2269 YBD=2987 YBD=2985 YBD=2992 YBD=2986 YBD=3001 YHG=2957>and YHG=2957>about YHG=2958>12 YHG=2958>hours YHG=2956>the YHG=2956>oedema YHG=2957>in YHG=2957>increasing nuid, " XBD=156 XBD=324 XBD=390 XBD=482 XBD=667 XBD=786 XBD=855 XBD=977 XBD=1178 XBD=1293 XHG=102 XHG=185 XHG=348 XHG=414 XHG=509 XHG=694 XHG=816 XHG=881 XHG=1004 XHG=1203 YBD=3008 YBD=3006 YBD=3007 YBD=3014 YBD=3006 YHG=2977>Cl. YHG=2977>welchii YHG=2977>in YHG=2977>the YHG=2976>relative YHG=2977>titre YHG=2977>of YHG=2977>both YHG=2986>enzymes YHG=2977>that " XBD=1536 XBD=1658 XBD=1827 XBD=1888 XBD=1972 XHG=1355 XHG=1557 XHG=1679 XHG=1846 XHG=1905 XHG=1991 YBD=3032 YBD=3026 YBD=3033 YBD=3024 YBD=3025 YHG=2995>quantity YHG=3005>more YHG=2996>rapidly YHG=2996>in YHG=2995>the YHG=2995>latter. " XBD=2120 XBD=306 XHG=779 XBD=365 XBD=449 XBD=609 XBD=706 XBD=764 XBD=846 XBD=1014 XBD=1075 XBD=1158 XBD=1292 XHG=100 XHG=323 XHG=381 XHG=468 XHG=628 XHG=723 XHG=868YBD=3045 XHG=1032 XHG=1092 XHG=1177 YBD=3053 YBD=3045 YBD=3045 YBD=3046 YHG=3016>developed YHG=3015>in YHG=3016>the YHG=3016>muscle YHG=3016>and YHG=3016>in YHG=3017>the YHG=3016>oedema YHG=3025>as YHG=3016>the YHG=3016>infec" XBD=2528 XBD=1470 XBD=1701 XBD=1900 XBD=2012 XBD=2080 XBD=2171 XBD=2339 XBD=2450 XHG=1397 XHG=1502 XHG=1733 XHG=1932 XHG=2043 XHG=2111 XHG=2205 XHG=2368 XHG=2477 YBD=3089 YBD=3080 YBD=3081 YHG=3052>Our YHG=3053>experience YHG=3037>indicates YHG=3053>that YHG=3053>in YHG=3052>the YHG=3053>oedema YHG=3053>fluid YHG=3054>the XBD=182 XHG=901 XBD=401 XBD=491 XBD=584 XBD=638 XBD=714 XBD=887 XBD=940 XBD=1091 XBD=1293 XHG=96 XHG=193 XHG=413 XHG=503 XHG=595 XHG=648 XHG=728 XHG=951 XHG=1106 YBD=3085 YBD=3093 YBD=3083 YBD=3084 YBD=3084 YBD=3092 YHG=3055>tion YHG=3055>proceeded YHG=3055>and YHG=3055>also YHG=3055>in YHG=3055>the YHG=3055>amount YHG=3055>of YHG=3055>muscle YHG=3054>digestion |>" " XBD=1496 XBD=1685 XBD=1795 XBD=1881 XBD=1989 XBD=2428 XBD=2530 XBD=2064 XHG=1351 XHG=1518 XHG=1705 XHG=1816 XHG=1901 XHG=2085 XHG=2446 XHG=2010 YBD=3126 YBD=3120 YBD=3128 YBD=3121 YBD=3120 YHG=3100>enzyme YHG=3092>increases YHG=3092>from YHG=3092>less YHG=3091>than YHG=3092>viscosity-reducing YHG=3092>units YHG=3093>0’5 XBD=175 XBD=272 XBD=439 XBD=511 XBD=608 XBD=801 XBD=897 XBD=961 XBD=1074 XBD=1291 XHG=97 XHG=206 XHG=304 XHG=471 XHG=541 XHG=643 XHG=861 XHG=928 XHG=992 XHG=1106 YBD=3124 YBD=3124 YBD=3123 YBD=3129 YHG=3095>and YHG=3095>the YHG=3094>extent YHG=3093>of YHG=3094>the YHG=3093>oedema. YHG=3093>It YHG=3094>is YHG=3094>not, YHG=3094>however, XBD=1480XHG=769 XBD=1538 XBD=1598 XBD=1719 XBD=1774 XBD=1901 XBD=1973 XBD=2090 XBD=2145 XBD=2207 XBD=2328 XBD=2413 XBD=2463 XBD=2530 XHG=1353 XHG=1499 XHG=1560 XHG=1614 XHG=1737 XHG=1792 XHG=1919 XHG=1989 XHG=2108 XHG=2165 XHG=2223 XHG=2345 XHG=2429 XHG=2477 YBD=3166 YBD=3160 YBD=3158 YBD=3159 YHG=3130>(VRU) YHG=3132>12 YHG=3134>to YHG=3132>about YHG=3131>2’0 YHG=3127>VRU YHG=3133>at YHG=3132>24 YHG=3131>hours YHG=3131>and YHG=3131>the " XBD=1292 XBD=284 XBD=355 XBD=528 XBD=644 XBD=739 XBD=985 XBD=1054 XHG=97 XHG=311 XHG=384 XHG=549 XHG=672 XHG=1013 XHG=1081 YBD=3170 YBD=3163 YBD=3161 YBD=3162 YBD=3162 YBD=3161 YHG=3134>proposed YHG=3134>to YHG=3133>discuss YHG=3127>’here YHG=3132>the YHG=3132>differences YHG=3132>in YHG=3132>individual " XBD=1481 XBD=1591 XBD=1675 XBD=1781 XBD=1855 XBD=2020 XBD=2057 XBD=2176 XBD=2232 XBD=2361 XHG=1350 XHG=1500 XHG=1610 XHG=1694 XHG=1800 XHG=1983 XHG=2038 XHG=2070 XHG=2195 XHG=2253 XHG=2379 YBD=3197 YBD=3198 YBD=3201 YHG=3169>muscle YHG=3169>from YHG=3170>less YHG=3170>than YHG=3171>0’5 YHG=3172>at YHG=3170>6 YHG=3169>hours YHG=3172>to YHG=3170>about YHG=3169>1’0 VRU " XBD=2527 XBD=310 XHG=888 XBD=471 XBD=507 XBD=648 XBD=732 XBD=871 XBD=966 XBD=1095 XBD=1234 XBD=1293 XHG=95 XHG=355 XHG=491 XHG=525 XHG=665 XHG=752 XHG=984 XHG=1112 XHG=1253 YBD=3202 YBD=3201 YBD=3208 YBD=3200 YBD=3201 YBD=3200 YHG=3172>infections. YHG=3171>Table YHG=3179>I YHG=3173>shows YHG=3171>the YHG=3180>upper YHG=3171>and YHG=3171>lower YHG=3171>limits YHG=3170>of XBD=192 XHG=744 XBD=385 XBD=582 XBD=643 XBD=726 XBD=877 XBD=1042 XBD=1159 XBD=1291 XHG=96 XHG=210 XHG=406 XHG=600 XHG=660 XHG=894 XHG=1060 XHG=1176 YBD=3240 YBD=3247 YBD=3241 YBD=3239 YBD=3246 YBD=3239 YHG=3211>both YHG=3220>enzymes YHG=3211>detected YHG=3211>at YHG=3210>the YHG=3210>stated YHG=3210>periods YHG=3209>after YHG=3210>infec" XBD=2529 XBD=1389 XBD=1456 XBD=1593 XBD=1677 XBD=1756 XBD=1870 XBD=1963 XBD=2012 XBD=2120 XBD=2211 XBD=2324 XBD=2454 XHG=1351 XHG=1413 XHG=1477 XHG=1639 XHG=1697 XHG=1778 XHG=1891 XHG=1984 XHG=2034 XHG=2150 XHG=2240 XHG=2351 XHG=2480 YBD=3236 YBD=3247 YBD=3245 YBD=3237 YHG=3211>at YHG=3209>24 YHG=3208>hours. YHG=3209>If YHG=3209>MCP YHG=3211>test YHG=3209>is YHG=3209>used YHG=3209>the YHG=3208>final YHG=3208>figure YHG=3208>for " XBD=2525 XBD=1488 XBD=1597 XBD=1842 XBD=1903 XBD=2074 XBD=2137 XBD=2182 XBD=2353 XBD=2413 XHG=1352 XHG=1510 XHG=1620 XHG=1866 XHG=1927 XHG=2099 XHG=2161 XHG=2205 XHG=2375 XHG=2431 YBD=3284 YBD=3275 YBD=3276 YBD=3278 YHG=3247>oedema YHG=3247>fluid YHG=3248>corresponds YHG=3251>to YHG=3248>activity YHG=3250>at YHG=3257>a YHG=3238>dilution YHG=3248>of YHG=3248>about XBD=179 XHG=820 XBD=274 XBD=326 XBD=459 XBD=582 XBD=664 XBD=799 XBD=859 XBD=1019 XBD=1292 XHG=93 XHG=196 XHG=290 XHG=342 XHG=476 XHG=598 XHG=683 XHG=877 XHG=1037 YBD=3278 YBD=3279 YBD=3280 YBD=3287 YBD=3278 YBD=3278 YBD=3286 YHG=3250>tion YHG=3250>and YHG=3248>is YHG=3250>based YHG=3259>upon YHG=3249>the YHG=3249>result YHG=3248>of YHG=3248>several YHG=3248>experiments " XBD=2527 XBD=1393 XBD=1453 XBD=1549 XBD=1625 XBD=1776 XBD=1821 XBD=1992 XBD=2055 XBD=2118 XBD=2187 XBD=2344 XBD=2422 XHG=1353 XHG=1411 XHG=1478 XHG=1571 XHG=1647 XHG=1800 XHG=1845 XHG=2015 XHG=2079 XHG=2135 XHG=2235 XHG=2367 XHG=2443 YBD=3313 YBD=3314 YBD=3316 YBD=3315 YBD=3317 YHG=3289>1 : YHG=3287>40 YHG=3286>and YHG=3286>for YHG=3287>muscle YHG=3296>a YHG=3287>dilution YHG=3287>of YHG=3288>1 YHG=3287>20. YHG=3287>There YHG=3296>are YHG=3296>some XBD=189 XHG=400 XBD=381 XBD=552 XHG=94 XHG=208 YBD=3317 YBD=3318 YHG=3288>with YHG=3288>different YHG=3289>strains.

XBD=184 XBD=280 XBD=345 XBD=456 XBD=566 XBD=691 XBD=745 XBD=789 XBD=902 XBD=1018 XBD=1231 XBD=1292 XHG=145 XHG=203 XHG=299 XHG=367 XHG=475 XHG=585 XHG=711 XHG=765 XHG=811 XHG=922 XHG=1039 XHG=1250 YBD=3354 YBD=3356 YBD=3355 YBD=3362 YBD=3356 YBD=3355 YBD=3356 YHG=3326>It YHG=3326>will YHG=3327>be YHG=3336>seen YHG=3328>that YHG=3327>there YHG=3326>is YHG=3335>a YHG=3335>very YHG=3325>wide YHG=3325>variation YHG=3326>in |>" " XBD=1590 XBD=1740 XBD=1849 XBD=1918 XBD=2122 XBD=2307 XBD=2367 XBD=2527 XBD=1400 XHG=1426 XHG=1614 XHG=1763 XHG=1862 XHG=1942 XHG=2144 XHG=2330 XHG=2389 XHG=1351 YBD=3360 YBD=3353 YBD=3354 YBD=3363 YBD=3354 YHG=3326>aepticuna YHG=3326>strains YHG=3326>with YHG=3331>-’an YHG=3326>abnormal YHG=3324>capacity YHG=3329>to YHG=3327>produce YHG=3325>01. " XBD=2526 XBD=1618 XBD=1712 XBD=1771 XBD=1976 XBD=2084 XBD=2204 XBD=2353 XBD=2433 XHG=1348 XHG=1641 XHG=1735 XHG=1794 XHG=1997 XHG=2107 XHG=2228 XHG=2375 XHG=2454 YBD=3399 YBD=3391 YBD=3392 YBD=3393 YBD=3394 YHG=3363>hyaluronidase YHG=3364>and YHG=3364>in YHG=3364>infections YHG=3365>with YHG=3365>these YHG=3365>strains YHG=3365>the YHG=3366>titre XBD=159 XHG=781 XBD=343 XBD=401 XBD=514 XBD=707 XBD=766 XBD=848 XBD=1005 XBD=1062 XBD=1292 XHG=93 XHG=178 XHG=361 XHG=418 XHG=532 XHG=724 XHG=865 XHG=1024 XHG=1079 YBD=3392 YBD=3401 YBD=3395 YBD=3402 YBD=3394 YBD=3393 YHG=3364>the YHG=3365>potency YHG=3366>of YHG=3366>both YHG=3374>enzymes YHG=3365>in YHG=3364>the YHG=3364>tissues YHG=3364>of YHG=3364>individual XBD=256 XHG=742 XBD=325 XBD=403 XBD=556 XBD=624 XBD=715 XBD=937 XBD=1035 XBD=1127 XBD=1291 XHG=93 XHG=282 XHG=351 XHG=429 XHG=584 XHG=648 XHG=965 XHG=1061 XHG=1155 YBD=3431 YBD=3431 YBD=3432 YBD=3440 YBD=3433 YBD=3437 YBD=3432 YHG=3402>animals YHG=3403>at YHG=3404>all YHG=3404>stages YHG=3404>of YHG=3404>the YHG=3402>infection, YHG=3402>but YHG=3403>steady " XBD=2540 XBD=1388 XBD=1552 XBD=1609 XBD=1690 XBD=1846 XBD=1952 XBD=2053 XBD=2138 XBD=2198 XBD=2301 XBD=2358 XBD=2419 XHG=1348 XHG=1406 XHG=1573 XHG=1629 XHG=1712 XHG=1865 XHG=1969 XHG=2073 XHG=2159 XHG=2217 XHG=2322 XHG=2382 XHG=2437 YBD=3437 YBD=3429 YBD=3430 YBD=3431 YBD=3439 YBD=3441 YBD=3432 YBD=3433 YHG=3402>of YHG=3410>enzyme YHG=3403>in YHG=3403>the YHG=3403>cedema YHG=3404>fluid YHG=3413>may YHG=3403>rise YHG=3412>as YHG=3404>high YHG=3413>as YHG=3405>10 YHG=3402>VRU’ " XBD=2457 XBD=1455 XBD=1580 XBD=1639 XBD=1733 XBD=1792 XBD=1910 XBD=2014 XHG=1348 XHG=1470 XHG=1600 XHG=1659 XHG=1753 XHG=1806 XHG=1926 XHG=2029 YBD=3478 YBD=3468 YBD=3477 YBD=3470 YBD=3471 YBD=3472 YHG=3441>(MCP YHG=3441>about YHG=3442>1 YHG=3442>100) YHG=3443>12 YHG=3441>hours YHG=3442>after YHG=3442>infection. : XBD=258 XHG=767 XBD=321 XBD=497 XBD=684 XBD=749 XBD=834 XBD=1039 XBD=1238 XBD=1291 XHG=93 XHG=279 XHG=341 XHG=520 XHG=705 XHG=856 XHG=1060 XHG=1258 YBD=3476 YBD=3469 YBD=3477 YBD=3479 YBD=3471 YBD=3470 YBD=3470 YHG=3440>increase YHG=3440>in YHG=3450>enzyme YHG=3442>activity YHG=3451>as YHG=3442>the YHG=3441>infection YHG=3441>proceeds YHG=3442>is

septicttm

VRU

357 " XBD=2469 XBD=401 XHG=285 XBD=1195 XBD=1447 XBD=1529 XBD=1630 XBD=1964 XBD=2123 XBD=2396 XHG=1140 XHG=1223 XHG=1475 XHG=1551 XHG=1657 XHG=1991 XHG=2148 XHG=2424 3I--I3YALURONIDASE-PRODUCTION YBD=238 YBD=235 YBD=232 YBD=233 YBD=234 YHG=212>TABLE YHG=213>BY YHG=211>ORGANISMS YHG=211>OF YHG=210>THE YHG=209>GAS-GANGRENE YHG=209>GROUP YHG=208>CULTIVATED YHG=209>IN ,

" XBD=1727 XBD=1078 XHG=1633 XBD=1182 XBD=1321 XBD=1607 XHG=1019 YBD=274 XHG=1105 XHG=1208 XHG=1348 YBD=273 YHG=250>ACID YHG=244>0-5 YHG=250>PER YHG=250>CENT. YHG=250>HYALURONIC

" XBD=2608 XBD=265 XHG=868 XBD=497 XBD=592 XBD=789 XBD=850 XBD=945 XBD=1058 XBD=1118 XBD=1257 XBD=1343 XBD=1503 XBD=1682 XBD=2008 XBD=2141 XBD=2192 XBD=2291 XBD=2361 XBD=2434 XHG=213 XHG=283 XHG=513 XHG=612 XHG=807 XHG=963YBD=1050 XHG=1077 XHG=1137 XHG=1276 XHG=1404 XHG=1533 XHG=1711 XHG=2051 XHG=2171 XHG=2223 XHG=2321 XHG=2389 XHG=2464 YBD=1043 YBD=1042 YBD=1042 YBD=1049 YBD=1050 YBD=1042 YBD=1041 YBD=1043 YHG=1013>No YHG=1012>haemolysin YHG=1021>was YHG=1012>detected YHG=1012>at YHG=1022>any YHG=1013>time YHG=1013>in YHG=1013>either YHG=1013>the YHG=1012>from YHG=1012>clinical YHG=1021>gas-gangrene YHG=1013>only YHG=1012>2 YHG=1012>out YHG=1012>of YHG=1012>20 YHG=1013>strains ; " XBD=1159 XBD=303 XBD=411 XBD=470 XBD=630 XBD=692 XBD=778 XBD=965 XHG=173 XHG=322 XHG=430 XHG=489 XHG=652 XHG=710 XHG=984 YBD=1083 YBD=1082 YBD=1082 YHG=1052>oedema YHG=1052>fluid YHG=1061>or YHG=1051>muscle YHG=1051>of YHG=1051>the YHG=1051>infected YHG=1052>animals. XBD=1493XHG=797 XBD=1557 XBD=1649 XBD=1820 XBD=1917 XBD=2085 XBD=2193 XBD=2394 XBD=2507 XBD=2604 XHG=1402 XHG=1509 XHG=1572 XHG=1656 XHG=1834 XHG=1930 XHG=2099 XHG=2210 XHG=2406 XHG=2521 YBD=1082 YBD=1081 YBD=1089 YHG=1051>that YHG=1051>do YHG=1052>not YHG=1051>produce YHG=1051>this YHG=1060>enzyme YHG=1061>were YHG=1051>obtained YHG=1051>from YHG=1061>gas" XBD=1592 XBD=1726 XBD=1828 XBD=1909 XBD=2068 XBD=2122 XBD=2193 XBD=2387 XBD=2491 XBD=2604 XHG=1403 XHG=1609 XHG=1765 XHG=1841 XHG=1925 XHG=2084 XHG=2140 XHG=2211 XHG=2400 XHG=2506 YBD=1122 YBD=1129 YBD=1121 YHG=1100>gangrene YHG=1100>cases. YHG=1091>All YHG=1091>the YHG=1090>strains YHG=1091>of YHG=1091>Cl. YHG=1091>septicum YHG=1091>that YHG=1091>have |>" " XBD=784 XBD=535 XHG=555 XHG=800 XHG=468 YBD=1151 YBD=1151 YBD=1151 YHG=1121>cedematiens YHG=1128>INFECTION YHG=1106>Cl. " XBD=1014 XBD=1501 XBD=1720 XBD=1898 XBD=2016 XBD=2339 XBD=2435 XBD=2605 XHG=1405 XHG=1515 XHG=1735 XHG=1915 XHG=2033 XHG=2381 XHG=2454 YBD=1168 YBD=1167 YBD=1161 YBD=1160 YHG=1131>been YHG=1130>examined YHG=1131>produce YHG=1139>some YHG=1130>hyaluronidase. YHG=1131>Cl. YHG=1130>wdemacde7natiens " XBD=2607 XBD=1497 XBD=1689 XBD=1732 XBD=1825 XBD=2094 XBD=2285 XBD=2411 XBD=2454 XBD=2545 XHG=1404 XHG=1516 XHG=1709 XHG=1752 XHG=1845 XHG=2114 XHG=2321 XHG=2434 XHG=2473 XHG=2567 YBD=1207 YBD=1199 YBD=1200 YHG=1170>tiens YHG=1170>presents YHG=1179>a YHG=1169>less YHG=1169>satisfactory YHG=1170>picture YHG=1170>only YHG=1171>7 YHG=1169>out YHG=1171>of : XBD=284 XHG=944 XBD=399 XBD=545 XBD=603 XBD=808 XBD=921 XBD=998 XBD=1344 XHG=212 XHG=301 XHG=418 XHG=565 XHG=622 XHG=825 XHG=1264 YBD=1213 YBD=1212 YBD=1205 YBD=1206 YBD=1206 YHG=1176>The YHG=1175>early YHG=1175>stages YHG=1175>of YHG=1175>infection YHG=1176>with YHG=1174>Cl. YHG=1176>also " XBD=2606 XBD=1450 XBD=1609 XBD=1788 XBD=1869 XBD=2040 XBD=2134 XBD=2254 XBD=2303 XBD=2369 XHG=1408 XHG=1466 XHG=1624 XHG=1803 XHG=1887 XHG=2056 XHG=2148 XHG=2269 XHG=2318 XHG=2384 YBD=1240 YBD=1247 YBD=1238 YBD=1239 YBD=1246 YHG=1210>15 YHG=1209>strains YHG=1209>produce YHG=1209>the YHG=1218>enzyme YHG=1209>and YHG=1209>there YHG=1209>is YHG=1219>no YHG=1208>suggestion XBD=358 XHG=888 XBD=478 XBD=630 XBD=751 XBD=865 XBD=941 XBD=1112 XBD=1237 XBD=1343 XHG=172 XHG=377 XHG=500 XHG=651 XHG=769 XHG=964YBD=1245 XHG=1159 XHG=1260 YBD=1244 YBD=1252 YBD=1246 YBD=1245 YHG=1213>developed YHG=1224>more YHG=1214>slowly YHG=1215>than YHG=1215>with YHG=1214>Cl. YHG=1215>welchii. YHG=1215>The YHG=1215>first " XBD=2607 XBD=1491 XBD=1599 XBD=1808 XBD=1869 XBD=2112 XBD=2234 XBD=2327 XBD=2534 XHG=1402 XHG=1519 XHG=1629 XHG=1835 XHG=1897 XHG=2138 XHG=2260 XHG=2358 XHG=2562 YBD=1286 YBD=1277 YBD=1285 YBD=1278 YHG=1249>that YHG=1248>this YHG=1249>property YHG=1249>is YHG=1247>associated YHG=1248>with YHG=1248>the YHG=1247>capacity YHG=1248>to XBD=403 XHG=820 XBD=527 XBD=641 XBD=800 XBD=919 XBD=1059 XBD=1103 XBD=1250 XBD=1344 XHG=170 XHG=423 XHG=546 XHG=664 XHG=940YBD=1284 XHG=1082 XHG=1124 XHG=1273 YBD=1291 YBD=1290 YBD=1284 YBD=1283 YBD=1290 YBD=1284 YHG=1253>macroscopic YHG=1253>signs YHG=1263>were YHG=1254>visible YHG=1254>after YHG=1254>about YHG=1255>6 YHG=1254>hours, YHG=1255>but " XBD=1346 XBD=303 XBD=407 XBD=533 XBD=617 XBD=679 XBD=879 XBD=951 XBD=1230 XHG=170 XHG=318 XHG=423 XHG=547 XHG=633 XHG=698 XHG=966 XHG=1245 YBD=1322 YBD=1324 YBD=1323 YBD=1323 YBD=1324 YHG=1293>cedema YHG=1292>fluid YHG=1293>could YHG=1293>not YHG=1293>be YHG=1293>collected YHG=1293>for YHG=1294>examination YHG=1294>until XBD=1515XHG=893 XBD=1818 XBD=1981 XBD=2216 XBD=2331 XBD=2378 XBD=2422 XBD=2540 XBD=2605 XHG=1405 XHG=1536 XHG=1857 XHG=1997 XHG=2227 XHG=2346 XHG=2388 XHG=2435 XHG=2558 YBD=1325 YBD=1317 YBD=1315 YBD=1316 YHG=1297>cause YHG=1297>gas-gangrene. YHG=1286>When YHG=1287>examining YHG=1287>table YHG=1294>11 YHG=1287>it YHG=1286>must YHG=1287>be " XBD=2606 XBD=1660 XBD=1775 XBD=1867 XBD=2087 XBD=2244 XBD=2432 XBD=2556 XHG=1403 XHG=1684 XHG=1800 XHG=1895 XHG=2113 XHG=2268 XHG=2456 XHG=2582 YBD=1357 YBD=1356 YBD=1363 YBD=1355 YHG=1327>remembered YHG=1327>that YHG=1327>the YHG=1326>organism YHG=1325>finally YHG=1325>isolated YHG=1326>from YHG=1335>a XBD=276 XHG=681 XBD=345 XBD=498 XBD=651 XBD=924 XBD=1019 XBD=1230 XBD=1342 XHG=170 XHG=305 XHG=374 XHG=551 XHG=953YBD=1368 XHG=1047 XHG=1259 YBD=1361 YBD=1362 YBD=1362 YBD=1370 YHG=1331>about YHG=1331>12 YHG=1332>hours. YHG=1333>Once YHG=1333>established, YHG=1332>the YHG=1333>infection YHG=1343>pro" XBD=2604 XBD=1543 XBD=1650 XBD=1737 XBD=1800 XBD=1883 XBD=1971 XBD=2078 XBD=2170 XBD=2355 XHG=1403 XHG=1558 XHG=1667 XHG=1753 XHG=1815 XHG=1902 XHG=1988 XHG=2093 XHG=2186 XHG=2370 YBD=1404 YBD=1396 YBD=1395 YBD=1403 YHG=1366>wound YHG=1376>may YHG=1366>not YHG=1366>be YHG=1366>the YHG=1375>one YHG=1365>that YHG=1374>was YHG=1365>actually YHG=1365>responsible XBD=293 XHG=710 XBD=402 XBD=571 XBD=689 XBD=868 XBD=1080 XBD=1170 XBD=1282 XBD=1343 XHG=156 XHG=314 XHG=422 XHG=592 XHG=891YBD=1402 XHG=1100 XHG=1193 XHG=1305 YBD=1401 YBD=1408 YBD=1409 YBD=1409 YBD=1410 YBD=1402 YHG=1371>ceeded YHG=1380>very YHG=1372>rapidly YHG=1371>with YHG=1372>marked YHG=1372>digestion YHG=1373>not YHG=1373>only YHG=1373>of " XBD=1761 XBD=1461 XBD=1545 XHG=1403 XHG=1477 XHG=1563 YBD=1436 YBD=1435 YBD=1443 YHG=1406>for YHG=1406>the YHG=1414>gangrene. XBD=225 XHG=875 XBD=368 XBD=547 XBD=726 XBD=810 XBD=863 XBD=926 XBD=1096 XBD=1343 XHG=167 XHG=237 XHG=380 XHG=559 XHG=739 XHG=824 XHG=937YBD=1441 XHG=1107 YBD=1440 YBD=1448 YBD=1441 YBD=1449 YHG=1410>the YHG=1411>muscle YHG=1410>initially YHG=1411>infected YHG=1411>but YHG=1412>of YHG=1412>all YHG=1411>muscles YHG=1411>throughout " XBD=2604 XBD=1572 XBD=1810 XBD=1982 XBD=2072 XBD=2369 XBD=2515 XHG=1455 XHG=1598 XHG=1835 XHG=2006 XHG=2100 XHG=2396 XHG=2541 YBD=1481 YBD=1482 YBD=1474 YHG=1444>Other YHG=1445>organisms YHG=1444>besides YHG=1444>the YHG=1453>gas-gangrene YHG=1453>group YHG=1453>are XBD=226 XHG=613 XBD=360 XBD=465 XBD=585 XBD=767 XBD=1026 XBD=1279 XBD=1344 XHG=168 XHG=252 XHG=387 XHG=489 XHG=795YBD=1488 XHG=1051 XHG=1306 YBD=1479 YBD=1487 YBD=1480 YBD=1481 YBD=1488 YBD=1488 YHG=1450>the YHG=1449>body, YHG=1451>and YHG=1451>with YHG=1450>copious YHG=1451>generalised YHG=1451>production YHG=1451>of " XBD=2605 XBD=1518 XBD=1584 XBD=1720 XBD=1883 XBD=2088 XBD=2186 XBD=2302 XBD=2416 XHG=1403 XHG=1540 XHG=1608 XHG=1743 XHG=1903 XHG=2109 XHG=2207 XHG=2324 XHG=2439 YBD=1513 YBD=1514 YBD=1521 YHG=1484>liable YHG=1484>to YHG=1493>cause YHG=1483>wound YHG=1483>infection YHG=1483>and YHG=1483>they YHG=1492>may YHG=1483>produce XBD=302 XHG=826 XBD=439 XBD=551 XBD=790 XBD=922 XBD=1099 XBD=1345 XHG=168 XHG=337 XHG=504 XHG=586 XHG=959YBD=1521 XHG=1136 YBD=1519 YBD=1520 YBD=1520 YBD=1527 YHG=1489>oedema YHG=1489>fluid. YHG=1490>In YHG=1490>infections YHG=1490>with YHG=1490>strains YHG=1490>producing " XBD=2606 XBD=1709 XBD=1867 XBD=2024 XBD=2079 XBD=2388 XHG=1404 XHG=1751 XHG=1883 XHG=2040 XHG=2095 XHG=2403 YBD=1553 YBD=1561 YHG=1523>hyaluronidase. YHG=1523>Many YHG=1523>strains YHG=1523>of YHG=1522>staphylococci, YHG=1523>especially XBD=452 XHG=578 XBD=548 XBD=790 XBD=1005 XBD=1073 XBD=1166 XBD=1344 XHG=167 XHG=481 XHG=816YBD=1567 XHG=1031 XHG=1099 XHG=1197 YBD=1566 YBD=1560 YBD=1568 YBD=1559YHG=1529>organisms YBD=1560 YHG=1528>hyaluronidase, YHG=1529>the YHG=1530>appeared YHG=1530>in YHG=1530>the YHG=1530>oedema XBD=1514 XBD=1626 XBD=1835 XBD=1993 XBD=2370 XBD=2469 XBD=2606 XBD=2229 XHG=1404 XHG=1534 XHG=1643 XHG=1856 XHG=2246 XHG=2390 XHG=2488 XHG=2011 YBD=1602 YBD=1593 YBD=1600 YBD=1601 YHG=1564>those YHG=1563>that YHG=1563>elaborate YHG=1563>potent YHG=1563>toxin, YHG=1563>and YHG=1571>many YHG=1563>haemolytic "|>"XBD=1344 XBD=251 XBD=371 XBD=423 XBD=565 XBD=769 XBD=876 XBD=1097 XBD=1274 XHG=169 XHG=277 XHG=402 XHG=451 XHG=596 XHG=798 XHG=903 XHG=1124 XHG=1302 YBD=1598 YBD=1599 YBD=1600 YBD=1600 YBD=1607 YBD=1599 YHG=1567>fluid YHG=1568>from YHG=1569>6 YHG=1569>hours YHG=1570>onwards YHG=1570>and YHG=1570>increased YHG=1570>rapidly YHG=1569>in " XBD=1548 XBD=1612 XBD=1854 XBD=2128 XBD=2191 XBD=2368 XBD=2608 XHG=1405 XHG=1573 XHG=1636 XHG=1879 XHG=2153 XHG=2216 XHG=2393 YBD=1633 YBD=1640 YBD=1632 YHG=1602>strains YHG=1603>haemolytic YHG=1602>streptococci YHG=1602>of YHG=1602>various YHG=1602>Lancefield |>" XBD=353 XBD=471 XBD=589 XBD=683 XBD=778 XBD=880 XBD=956 XBD=1172 XBD=1345 XHG=168 XHG=391 XHG=497 XHG=615 XHG=707 XHG=803 XHG=905 XHG=980 XHG=1222 YBD=1643 YBD=1638 YBD=1639 YBD=1646 YBD=1639 YHG=1607>numbers YHG=1608>this YHG=1608>fluid YHG=1609>did YHG=1609>not YHG=1609>clot YHG=1618>on YHG=1609>standing. YHG=1600>When; " XBD=2605 XBD=1545 XBD=1650 XBD=1837 XBD=2035 XBD=2099 XBD=2203 XBD=2318 XBD=2479 XHG=1406 XHG=1568 XHG=1674 XHG=1862 XHG=2061 XHG=2121 XHG=2225 XHG=2341 XHG=2503 YBD=1671 YBD=1679 YBD=1677 YBD=1672 YHG=1651>groups YHG=1642>also YHG=1642>produce YHG=1651>enzymes YHG=1642>of YHG=1641>this YHG=1641>type YHG=1642>which, YHG=1642>howXBD=296 XHG=767 XBD=404 XBD=518 XBD=661 XBD=748 XBD=836 XBD=1019 XBD=1343 XHG=167 XHG=312 XHG=425 XHG=536 XHG=682 XHG=856YBD=1678 XHG=1039 YBD=1676 YBD=1677 YBD=1686 YBD=1685YHG=1648>not YHG=1646>strains YHG=1656>were YHG=1647>used YHG=1648>which YHG=1648>did YHG=1648>produce YHG=1648>hyaluronidase, XBD=1504XHG=881 XBD=1592 XBD=1886 XBD=2080 XBD=2324 XBD=2533 XBD=2607 XHG=1405 XHG=1529 XHG=1615 XHG=1910 XHG=2127 XHG=2347 XHG=2554 YBD=1719 YBD=1713 YBD=1711 YBD=1718 YHG=1691>ever, YHG=1691>are YHG=1681>antigenically YHG=1681>distinct. YHG=1681>Therefore YHG=1680>infection YHG=1682>by " XBD=1344 XBD=224 XBD=388 XBD=505 XBD=605 XBD=856 XBD=983 XBD=1189 XBD=1255 XHG=165 XHG=249 XHG=414 XHG=528 XHG=631 XHG=1008 XHG=1211 XHG=1277 YBD=1715 YBD=1716 YBD=1725 YBD=1717 YBD=1717 YHG=1686>the YHG=1685>oedema YHG=1686>fluid YHG=1696>was YHG=1687>gelatinous, YHG=1696>more YHG=1687>localised YHG=1687>to YHG=1687>the " XBD=2608 XBD=1511 XBD=1763 XBD=1890 XBD=2014 XBD=2154 XBD=2257 XBD=2529 XHG=1404 XHG=1551 XHG=1798 XHG=1925 XHG=2044 XHG=2190 XHG=2294 XHG=2569 YBD=1750 YBD=1758 YBD=1759 YBD=1751 YHG=1721>these YHG=1721>organisms YHG=1730>may YHG=1721>also. YHG=1730>cause YHG=1720>the YHG=1730>appearance YHG=1721>of XBD=319 XHG=842 XBD=431 XBD=527 XBD=594 XBD=824 XBD=952 XBD=1016 XBD=1122 XBD=1179 XBD=1227 XBD=1346 XHG=166 XHG=333 XHG=449 XHG=544 XHG=612 XHG=969YBD=1758 XHG=1034 XHG=1137 XHG=1194 XHG=1243 YBD=1762 YBD=1756 YBD=1757 YBD=1764 YBD=1757 YBD=1757 YBD=1756 YHG=1725>infected YHG=1735>area, YHG=1726>and YHG=1736>no YHG=1727>organisms YHG=1727>could YHG=1727>be YHG=1736>seen YHG=1727>in YHG=1727>it YHG=1727>until " XBD=2606 XBD=1699 XBD=1762 XBD=1850 XBD=2011 XBD=2211 XBD=2312 XBD=2378 XBD=2479 XHG=1405 XHG=1719 XHG=1784 XHG=1871 XHG=2034 XHG=2235 XHG=2336 XHG=2400 XHG=2501 YBD=1790 YBD=1791 YBD=1799 YBD=1798 YHG=1761>hyaluronidase YHG=1760>the YHG=1761>wound YHG=1761>exudate, YHG=1761>and YHG=1761>in YHG=1771>any YHG=1761>diagXBD=223 XHG=765 XBD=420 XBD=574 XBD=642 XBD=736 XBD=960 XBD=1105 XBD=1344 XHG=165 XHG=249 XHG=447 XHG=603 XHG=669 XHG=1014 XHG=1133 YBD=1795 YBD=1803 YBD=1796 YBD=1797 YBD=1796 YBD=1803 YHG=1766>the YHG=1766>terminal YHG=1766>stages YHG=1766>of YHG=1767>the YHG=1766>infection. YHG=1767>This YHG=1766>gelatinous XBD=1531XHG=902 XBD=1749 XBD=1882 XBD=2000 XBD=2079 XBD=2288 XBD=2341 XBD=2435 XBD=2606 XHG=1406 XHG=1544 XHG=1764 XHG=1895 XHG=2012 XHG=2095 XHG=2302 XHG=2354 XHG=2450 YBD=1838 YBD=1830 YBD=1831 YBD=1837 YHG=1799>nostic YHG=1800>procedure YHG=1800>based YHG=1795>upon YHG=1800>the YHG=1800>detection YHG=1800>of YHG=1801>this YHG=1809>enzyme " XBD=1344 XBD=249 XBD=331 XBD=417 XBD=499 XBD=669 XBD=883 XBD=940 XBD=1023 XBD=1135 XHG=167 XHG=265 XHG=348 XHG=433 XHG=519 XHG=689 XHG=957 XHG=1043 XHG=1153 YBD=1836 YBD=1835 YBD=1835 YBD=1835 YBD=1844 YHG=1801>fluid YHG=1805>has YHG=1804>not YHG=1805>the YHG=1805>viscous YHG=1806>character YHG=1805>of YHG=1805>the YHG=1805>fluid YHG=1805>produced toc7n4 " XBD=2608 XBD=1438 XBD=1538 XBD=1607 XBD=1818 XBD=1886 XBD=2118 XBD=2266 XBD=2328 XBD=2417 XHG=1404 XHG=1460 XHG=1562 XHG=1634 XHG=1842 XHG=1911 XHG=2141 XHG=2289 XHG=2350 XHG=2442 YBD=1869 YBD=1870 YBD=1878 YHG=1839>it YHG=1839>will YHG=1840>be YHG=1840>desirable YHG=1840>to YHG=1839>determine YHG=1840>which YHG=1840>of YHG=1840>the YHG=1840>possible XBD=202 XHG=339 XBD=299 XBD=627 XBD=1028 XBD=1136 XBD=1252 XBD=1344 XHG=165 XHG=235 XHG=842YBD=1882 XHG=1066 XHG=1174 XHG=1291 YBD=1874 YBD=1875 YBD=1875 YBD=1876 YBD=1874 YHG=1844>in YHG=1844>the YHG=1838>corresponding YHG=1844>infection YHG=1854>nor YHG=1854>was YHG=1845>its " XBD=2609 XBD=1590 XBD=1757 XBD=1844 XBD=2079 XBD=2133 XBD=2199 XBD=2287 XBD=2464 XHG=1406 XHG=1615 XHG=1781 XHG=1870 XHG=2101 XHG=2157 XHG=2221 XHG=2312 XHG=2515 YBD=1916 YBD=1908 YBD=1910 YBD=1909 YHG=1878>bacterial YHG=1878>species YHG=1888>are YHG=1879>producing YHG=1879>it YHG=1879>in YHG=1879>the YHG=1880>wound. YHG=1879>This XBD=362 XHG=802 XBD=570 XBD=789 XBD=854 XBD=1170 XHG=164 XHG=377 XHG=585 XHG=868YBD=1922 YBD=1921 YBD=1914 YBD=1922 YHG=1883>gelatinous YHG=1884>character YHG=1883>destroyed YHG=1884>by YHG=1884>hyaluronidase. " XBD=2608 XBD=1479 XBD=1535 XBD=1646 XBD=1711 XBD=1871 XBD=2177 XBD=2226 XBD=2303 XHG=1406 XHG=1489 XHG=1549 XHG=1659 XHG=1723 XHG=1883 XHG=2187 XHG=2236 XHG=2314 YBD=1947 YBD=1948 YBD=1954 YBD=1949 YBD=1956 YHG=1927>can YHG=1918>be YHG=1917>done YHG=1918>by YHG=1918>specific YHG=1918>neutralisation YHG=1919>of YHG=1919>the YHG=1919>hyaluronidase XBD=442 XHG=844 XBD=662 XBD=741 XBD=827 XBD=942 XBD=1203 XBD=1261 XBD=1344 XHG=210 XHG=458 XHG=679 XHG=760 XHG=959YBD=1953 XHG=1221 XHG=1277 YBD=1954 YBD=1961 YBD=1953 YBD=1952 YHG=1922>(Edematiens YHG=1923>infections YHG=1933>are YHG=1924>the YHG=1923>least YHG=1923>satisfactory YHG=1924>of YHG=1923>the " XBD=2609 XBD=1458 XBD=1728 XBD=1920 XBD=2023 XBD=2071 XBD=2228 XBD=2362 XBD=2544 XHG=1407 XHG=1483 XHG=1752 XHG=1944 XHG=2046 XHG=2094 XHG=2252 XHG=2385 XHG=2570 YBD=1994 YBD=1987 YBD=1995 YBD=1996 YBD=1988 YHG=1957>by YHG=1957>appropriate YHG=1958>antisera YHG=1958>and YHG=1967>a YHG=1958>simple YHG=1958>rapid YHG=1958>method YHG=1958>of XBD=420 XHG=852 XBD=577 XBD=720 XBD=817 XBD=963 XBD=1035 XBD=1168 XBD=1237 XBD=1344 XHG=165 XHG=459 XHG=619 XHG=752 XHG=996YBD=2000 XHG=1068 XHG=1198 XHG=1277 YBD=2001 YBD=1993 YBD=1992 YBD=1992 YHG=1964>gas-gangrene YHG=1972>group YHG=1963>from YHG=1963>the YHG=1962>point YHG=1962>of YHG=1962>view YHG=1962>of YHG=1962>the " XBD=2608 XBD=1639 XBD=1733 XBD=1826 XBD=1918 XBD=1978 XBD=2189 XBD=2246 XBD=2324 XBD=2434 XHG=1407 XHG=1651 XHG=1747 XHG=1839 XHG=1931 XHG=1996 XHG=2202 XHG=2257 XHG=2339 XHG=2450 YBD=2034 YBD=2026 YBD=2027 YBD=2028 YHG=1996>performing YHG=1996>this YHG=1996>test YHG=1997>will YHG=1997>be YHG=1997>described YHG=1999>in YHG=1998>the YHG=1998>next YHG=1998>section. XBD=346 XHG=794 XBD=409 XBD=607 XBD=676 XBD=766 XBD=896 XBD=1034 XBD=1174 XBD=1243 XBD=1346 XHG=166 XHG=370 XHG=434 XHG=633 XHG=700 XHG=923YBD=2040 XHG=1059 XHG=1200 XHG=1267 YBD=2039 YBD=2032 YBD=2031 YBD=2032 YBD=2037 YBD=2031 YHG=2001>detection YHG=2001>of YHG=2011>enzymes YHG=2003>in YHG=2002>the YHG=2002>body YHG=2001>fluids YHG=2002>since, YHG=2011>as YHG=2001>will

"" XBD=1345 XBD=295 XBD=424 XBD=473 XBD=600 XBD=850 XBD=914 XBD=1080 XBD=1268 XHG=164 XHG=317 XHG=449 XHG=498 XHG=626 XHG=876 XHG=939 XHG=1104 XHG=1294 YBD=2079 YBD=2077 YBD=2070 YBD=2080 YBD=2071 YBD=2072 YBD=2079 YBD=2070 YHG=2050>appear YHG=2040>later, YHG=2050>a YHG=2042>large YHG=2041>proportion YHG=2040>of YHG=2040>strains YHG=2041>produce YHG=2049>no XBD=1812 XBD=1895 XBD=2116 XBD=2178 XHG=1630 XHG=1832 XHG=1915 XHG=2138 XHG=2195 YBD=2091 YBD=2090 YBD=2099 YHG=2059>Methods YHG=2061>for YHG=2062>Detection YHG=2061>of YHG=2063>Enzymes " XBD=2388 XBD=441 XHG=821 XBD=545 XBD=680 XBD=795 XBD=871 XBD=1060 XBD=1165 XBD=1344 XHG=165 XHG=466 XHG=568 XHG=706 XHG=897YBD=2110 XHG=1085 XHG=1190 YBD=2118 YBD=2111 YBD=2118 YBD=2110 YBD=2118 YHG=2080>Hyaluronidase YHG=2081>and YHG=2081>those YHG=2080>that YHG=2080>do YHG=2081>produce YHG=2080>this YHG=2089>enzyme XBD=1762 XBD=1880 XBD=1953 XBD=2159 XBD=2305 XBD=2384 XBD=2478 XHG=1456 XHG=1789 XHG=1907 XHG=1983 XHG=2184 XHG=2331 XHG=2410 XHG=2507 YBD=2151 YBD=2153 YBD=2147 YBD=2154 YBD=2146 YHG=2114>Hyaluronidase YHG=2124>may YHG=2115>be YHG=2115>detected YHG=2115>either YHG=2116>by YHG=2117>the YHG=2116>MCP " XBD=2608 XBD=295 XHG=902 XBD=345 XBD=404 XBD=519 XBD=649 XBD=763 XBD=881 XBD=956 XBD=1162 XBD=1242 XBD=1345 XHG=166 XHG=314 XHG=363 XHG=423 XHG=536 XHG=667 XHG=781 XHG=977YBD=2150 XHG=1206 XHG=1259 YBD=2151 YBD=2149 YBD=2158 YBD=2150 YBD=2149 YHG=2121>secrete YHG=2120>it YHG=2121>in YHG=2130>even YHG=2120>lower YHG=2120>titre YHG=2120>than YHG=2119>Cl. YHG=2120>septicum. YHG=2121>A YHG=2119>leci"|>"XBD=2608 XBD=1532 XBD=1599 XBD=1677 XBD=1766 XBD=2453 XBD=2518 XBD=2171 XHG=1406 XHG=1558 XHG=1624 XHG=1700 XHG=2193 XHG=2478 XHG=2540 XHG=1792 YBD=2183 YBD=2191 YBD=2184 YBD=2193 YBD=2185 YBD=2193 YHG=2153>test YHG=2163>or YHG=2155>by YHG=2155>activity YHG=2155>of YHG=2155>the YHG=2155>viscosity-reducing 18 18 19 XBD=304 XBD=344 XBD=433 XBD=640 XBD=690 XBD=792 XBD=874 XBD=976 XBD=1043 XBD=1132 XBD=1345 XHG=164 XHG=312 XHG=353 XHG=445 XHG=649 XHG=701 XHG=802 XHG=883 XHG=990 XHG=1052 XHG=1143 YBD=2190 YBD=2197 YBD=2196 YBD=2189 YBD=2197 YBD=2189 YBD=2196 YHG=2161>thinase YHG=2160>is YHG=2160>also YHG=2159>produced YHG=2160>in YHG=2168>very YHG=2160>low YHG=2158>titre YHG=2160>by YHG=2159>this YHG=2160>organism. " XBD=2607 XBD=1578 XBD=1712 XBD=1847 XBD=1957 XBD=2063 XBD=2141 XBD=2321 XBD=2421 XBD=2497 XHG=1409 XHG=1633 XHG=1746 XHG=1878 XHG=1992 XHG=2095 XHG=2177 XHG=2354 XHG=2454 XHG=2529 YBD=2230 YBD=2223 YBD=2225 YBD=2224 YBD=2232 YHG=2202>enzyme. YHG=2194>The YHG=2193>MCP YHG=2193>test YHG=2204>can YHG=2195>be YHG=2194>carried YHG=2195>out YHG=2194>in YHG=2203>any XBD=210 XHG=900 XBD=416 XBD=634 XBD=698 XBD=870 XBD=952 XBD=1007 XBD=1376 XHG=168 XHG=239 XHG=448 XHG=664 XHG=730 XHG=983YBD=2236 XHG=1039 YBD=2235 YBD=2234 YBD=2229 YBD=2228 YBD=2228 YBD=2236 YHG=2199>If, YHG=2200>however, YHG=2199>infection YHG=2198>is YHG=2198>caused YHG=2198>by YHG=2208>a YHG=2198>hyaluronidase" XBD=2611 XBD=1628 XBD=1750 XBD=1954 XBD=2213 XBD=2310 XHG=1406 XHG=1653 XHG=1776 XHG=1981 XHG=2241 XHG=2337 YBD=2269 YBD=2271 YBD=2263 YHG=2232>laboratory YHG=2233>with YHG=2233>ordinary YHG=2234>equipment, YHG=2233>but YHG=2234>viscosimetric XBD=362 XHG=932 XBD=510 XBD=617 XBD=802 XBD=904 XBD=978 XBD=1185 XBD=1253 XBD=1346 XHG=167 XHG=388 XHG=537 XHG=646 XHG=831 XHG=1010 XHG=1210 XHG=1279 YBD=2276 YBD=2275 YBD=2268 YBD=2267 YBD=2267 YHG=2239>producing YHG=2238>strain YHG=2238>this YHG=2247>enzyme YHG=2247>can YHG=2238>be YHG=2237>detected YHG=2237>in YHG=2237>the " XBD=1347 XBD=1520XHG=822 XBD=1571 XBD=1647 XBD=1814 XBD=2013 XBD=2160 XBD=2377 XBD=2611 XBD=304 XBD=358 XBD=466 XBD=520 XBD=722 XBD=809 XBD=869 XBD=1067 XBD=1139 XHG=168 XHG=317 XHG=372 XHG=477 XHG=535 XHG=738 XHG=1407YBD=2306 XHG=1532 XHG=1579 XHG=1658 XHG=1825 XHG=2021 XHG=2170 XHG=2387 XHG=886 XHG=1080 XHG=1152 YBD=2308 YBD=2314 YBD=2311 YBD=2307 YBD=2306 YBD=2309YHG=2277>be YBD=2301 YBD=2302 YBD=2311 YBD=2303 YBD=2310 YBD=2306 YBD=2307 YHG=2278>cedema YHG=2287>soon YHG=2287>as YHG=2277>sufficient YHG=2286>can YHG=2276>collected YHG=2280>assay YHG=2272>of YHG=2271>the YHG=2282>enzyme YHG=2273>normally YHG=2273>entails YHG=2273>somewhat YHG=2272>specialised YHG=2276>for YHG=2276>examina" XBD=2611 XBD=1632 XBD=1731 XBD=1965 XBD=2081 XBD=2143 XBD=2225 XBD=2357 XBD=2550 XHG=1408 XHG=1651 XHG=1748 XHG=2011 XHG=2104 XHG=2165 XHG=2246 XHG=2376 XHG=2569 YBD=2349 YBD=2342 YBD=2347 YBD=2348 YBD=2340 YHG=2311>equipment YHG=2312>and YHG=2312>technique. YHG=2312>Mr. YHG=2312>C. YHG=2311>W. YHG=2311>Hale, YHG=2312>working YHG=2312>in XBD=360 XHG=697 XBD=474 XBD=612 XBD=677 XBD=821 XHG=166 XHG=376 XHG=491 XHG=633 YBD=2353 YBD=2347 YBD=2345 YBD=2346 YHG=2317>tion-i.e., YHG=2311>after YHG=2316>about YHG=2316>12 YHG=2317>hours. " XBD=1489 XBD=1758 XBD=1877 XBD=2099 XBD=2356 XBD=2414 XBD=2611 XHG=1408 XHG=1524 XHG=1795 XHG=1914 XHG=2136 XHG=2391 XHG=2451 YBD=2388 YBD=2386 YBD=2382 YBD=2380 YBD=2381 YHG=2350>this YHG=2351>laboratory, YHG=2352>has, YHG=2352>however, YHG=2351>elaborated YHG=2360>a YHG=2350>method |>" " XBD=248 XBD=337 XBD=455 XBD=576 XBD=625 XBD=713 XBD=824 XBD=1185 XBD=1278 XBD=902 XHG=204 XHG=269 XHG=358 XHG=476 XHG=596 XHG=637 XHG=736 XHG=923 XHG=1206 XHG=1299 XHG=847 YBD=2399 YBD=2407 YBD=2400 YBD=2398 YBD=2406 YBD=2398 YBD=2406 YHG=2372>At YHG=2370>this YHG=2373>stage YHG=2371>there YHG=2370>is YHG=2370>less YHG=2369>than YHG=2369>VRU YHG=2370>0-5 YHG=2369>and YHG=2370>by present "" XBD=1349 XBD=1586 XBD=1672 XBD=1883 XBD=2183 XBD=2613 XHG=1388 XHG=1619 XHG=1706 XHG=1919 XHG=2214 YBD=2421 YBD=2428 YBD=2420 YBD=2427 YHG=2387>‘ YHG=2400>an YHG=2390>ordinary YHG=2390>Thoma-Zeiss YHG=2389>leucocyte-counting whereby XBD=219 XBD=327 XBD=419 XBD=503 XBD=681 XBD=763 XBD=828 XBD=1015 XBD=1074 XBD=1118 XBD=1290 XBD=1354 XHG=165 XHG=240 XHG=349 XHG=443 XHG=527 XHG=695 XHG=785 XHG=852 XHG=1036 XHG=1096 XHG=1141 XHG=1313 YBD=2439 YBD=2446 YBD=2437 YBD=2438 YBD=2437 YBD=2436 YBD=2437 YHG=2411>MCP YHG=2412>test YHG=2410>the YHG=2418>enzyme. YHG=2418>can YHG=2408>be YHG=2409>detected YHG=2409>in YHG=2418>a YHG=2409>dilution YHG=2408>of " XBD=1557 XBD=1669 XBD=1737 XBD=1853 XBD=1915 XBD=1959 XBD=2173 XBD=2466 XBD=2567 XBD=2612 XHG=1410 XHG=1577 XHG=1691 XHG=1758 XHG=1872 XHG=1935 XHG=1980 XHG=2195 XHG=2488 XHG=2588 YBD=2467 YBD=2464 YBD=2459 YBD=2458 YHG=2428>pipette YHG=2439>may YHG=2430>be YHG=2430>used YHG=2438>as YHG=2429>primitive YHG=2429>viscosimeter, YHG=2429>and YHG=2438>a 1: XHG=904 |>" " XBD=293 XBD=403 XBD=504 XBD=570 XBD=697 XBD=781 XBD=884 XBD=944 XBD=1022 XBD=1290 XBD=1350 XBD=1187 XHG=219 XHG=317 XHG=449 XHG=529 XHG=590 XHG=717 XHG=800 XHG=961 XHG=1206 XHG=1311 XHG=1042 YBD=2479 YBD=2478 YBD=2486 YBD=2476 YBD=2477 YBD=2477 YBD=2476 YBD=2485 YHG=2451>4 YHG=2450>1 YHG=2449>By YHG=2450>18 YHG=2450>hours YHG=2448>the YHG=2449>titre YHG=2448>of YHG=2448>the YHG=2449>rises YHG=2451>to YHG=2448>enzyme to8. : " XBD=1645 XBD=1720 XBD=1827 XBD=1891 XBD=1995 XBD=2176 XBD=2276 XBD=2345 XBD=2547 XBD=2613 XHG=1412 XHG=1668 XHG=1745 XHG=1851 XHG=1914 XHG=2018 XHG=2198 XHG=2298 XHG=2369 XHG=2571 YBD=2506 YBD=2497 YBD=2498 YHG=2469>description YHG=2469>by YHG=2469>him YHG=2469>of YHG=2468>this YHG=2468>method YHG=2468>will YHG=2468>be YHG=2468>included YHG=2468>in |>" " XBD=265 XBD=455 XBD=512 XBD=555 XBD=666 XBD=836 XBD=904 XBD=1037 XBD=1100 XBD=1170 XBD=1262 XBD=1349 XBD=336 XHG=165 XHG=355 XHG=474 XHG=532 XHG=573 XHG=686 XHG=864 XHG=927 XHG=1060 XHG=1120 XHG=1216 XHG=1288 XHG=287 YBD=2518 YBD=2517 YBD=2516 YBD=2516 YBD=2518 YBD=2517 YBD=2516 YHG=2490>about YHG=2489>VRU YHG=2498>or YHG=2498>a YHG=2488>MCP YHG=2488>dilution YHG=2487>of YHG=2488>about YHG=2490>1-0 YHG=2489>1 YHG=2488>16. YHG=2489>In YHG=2488>the : " XBD=1490 XBD=1670 XBD=1828 XBD=1874 XBD=2124 XBD=2335 XBD=2613 XHG=1410 XHG=1512 XHG=1720 XHG=1850 XHG=1897 XHG=2146 XHG=2356 YBD=2538 YBD=2537 YBD=2544 YHG=2508>this YHG=2508>section. YHG=2506>With YHG=2517>a YHG=2507>few,simple YHG=2507>technical YHG=2507>precautions, " XBD=285 XBD=367 XBD=537 XBD=591 XBD=691 XBD=912 XBD=986 XBD=1101 XBD=1286 XBD=1352 XHG=166 XHG=309 XHG=392 XHG=562 XHG=616 XHG=717 XHG=937 XHG=1012 XHG=1124 XHG=1313 YBD=2565 YBD=2557 YBD=2556 YBD=2564 YBD=2556 YBD=2555 YHG=2529>muscle YHG=2528>the YHG=2537>enzyme YHG=2528>is YHG=2528>just YHG=2527>detectable YHG=2527>by YHG=2527>both YHG=2527>methods YHG=2530>at " XBD=1476 XBD=1565 XBD=1626 XBD=1727 XBD=1894 XBD=2046 XBD=2098 XBD=2283 XBD=2349 XBD=2478 XBD=2568 XBD=2613 XHG=1410 XHG=1497 XHG=1586 XHG=1645 XHG=1747 XHG=1915 XHG=2065 XHG=2119 XHG=2303 XHG=2370 XHG=2500 XHG=2589 YBD=2577 YBD=2582 YBD=2575 YBD=2583 YBD=2584 YBD=2576 YHG=2547>the YHG=2557>use YHG=2547>of YHG=2547>this YHG=2546>pipette YHG=2546>makes YHG=2546>it YHG=2546>possible YHG=2546>to YHG=2555>carry YHG=2547>out YHG=2556>a " XBD=185 XBD=302 XBD=391 XBD=470 XBD=574 XBD=764 XBD=906 XBD=963 XBD=1095 XBD=1171 XBD=1292 XBD=1351 XHG=168 XHG=204 XHG=322 XHG=410 XHG=490 XHG=594 XHG=785 XHG=925 XHG=985 XHG=1117 XHG=1191 XHG=1313 YBD=2597 YBD=2596 YBD=2595 YBD=2602 YBD=2594 YBD=2595 YBD=2600 YBD=2596 YBD=2595 YHG=2569>6 YHG=2569>hours YHG=2567>and YHG=2567>the YHG=2568>titre YHG=2567>increases YHG=2567>slowly YHG=2569>to YHG=2567>about YHG=2569>0,5 YHG=2567>VRU YHG=2576>or " XBD=1521 XBD=1618 XBD=1756 XBD=1864 XBD=2098 XBD=2175 XBD=2259 XBD=2454 XBD=2514 XHG=1410 XHG=1538 XHG=1636 XHG=1775 XHG=1883 XHG=2116 XHG=2192 XHG=2279 XHG=2476 XHG=2532 YBD=2624 YBD=2622 YBD=2616 YBD=2615 YHG=2587>rapid YHG=2586>test YHG=2586>under YHG=2586>field YHG=2585>conditions YHG=2585>for YHG=2585>the YHG=2594>presence YHG=2586>of YHG=2586>this "" XBD=2615 |>" XBD=206 XBD=238 XBD=346 XBD=517 XBD=576 XBD=641 XHG=171 XHG=221 XHG=259 XHG=368 XHG=538 XHG=596 YBD=2636 YBD=2635 YBD=2642 hours. YHG=2607>1 YHG=2608>8 YHG=2607>MCP YHG=2606>dilution YHG=2610>at : XBD=1580 XHG=2569 XBD=1911 XBD=2019 XBD=2248 XBD=2349 XBD=2420 XBD=2544 XBD=2614 XHG=1411 YBD=2654 XHG=1633 XHG=1938 XHG=2044 XHG=2277 XHG=2375 XHG=2449 YBD=2663 YHG=2602>18 YBD=2660 YBD=2653 YHG=2635>enzyme. YHG=2625>Furthermore, YHG=2624>this YHG=2624>technique YHG=2633>can YHG=2625>be YHG=2625>used YHG=2625>to " XBD=1348 XBD=327 XBD=368 XBD=510 XBD=570 XBD=648 XBD=900 XBD=1058 XBD=1120 XBD=1304 XHG=213 XHG=344 XHG=389 XHG=531 XHG=593 XHG=671 XHG=918 XHG=1076 XHG=1141 XHG=1324 YBD=2697 YBD=2690 YBD=2691 YBD=2690 YBD=2699 YBD=2690 YHG=2661>Using YHG=2670>a YHG=2661>strain YHG=2661>of YHG=2660>Cl. YHG=2660>cedematiens YHG=2660>known YHG=2660>to YHG=2661>produce YHG=2670>a XBD=1540 XHG=2477 XBD=1621 XBD=1786 XBD=2093 XBD=2145 XBD=2224 XBD=2394 XBD=2463 XBD=2613 XHG=1413 YBD=2703 XHG=1553 XHG=1636 XHG=1799 XHG=2107 XHG=2158 XHG=2239 XHG=2410 YBD=2695 YHG=2673>approYBD=2693 YBD=2692 YBD=2700 YBD=2701 YHG=2664>detect YHG=2665>the YHG=2663>specific YHG=2662>neutralisation YHG=2662>of YHG=2662>the YHG=2672>enzyme YHG=2664>by XBD=352 XHG=856 XBD=466 XBD=711 XBD=827 XBD=898 XBD=1071 XBD=1173 XBD=1348 XHG=168 XHG=374 XHG=486 XHG=732 XHG=926YBD=2729 XHG=1092 XHG=1194 YBD=2737 YBD=2730 YBD=2729 YBD=2730 YBD=2738 YHG=2700>relatively YHG=2700>high YHG=2699>lecithinase YHG=2699>titre YHG=2699>in YHG=2699>culture YHG=2700>this YHG=2709>enzyme " XBD=2614 XBD=1535 XBD=1727 XBD=1827 XBD=1940 XBD=2006 XBD=2209 XBD=2298 XBD=2459 XBD=2523 XHG=1411 XHG=1558 XHG=1749 XHG=1848 XHG=1962 XHG=2030 XHG=2232 XHG=2322 XHG=2485 XHG=2544 YBD=2741 YBD=2739 YBD=2731 YBD=2733 YBD=2732 YHG=2703>priate YHG=2703>antisera YHG=2702>and YHG=2701>thus YHG=2701>to YHG=2702>diagnose YHG=2702>the YHG=2702>nature YHG=2702>of YHG=2703>the XBD=272 XHG=811 XBD=378 XBD=444 XBD=644 XBD=704 XBD=789 XBD=959 XBD=1072 XBD=1211 XBD=1349 XHG=172 XHG=291 XHG=399 XHG=467 XHG=661 XHG=721 XHG=979YBD=2769 XHG=1090 XHG=1232 YBD=2776 YBD=2768 YBD=2769 YBD=2769 YBD=2772 YHG=2739>could YHG=2739>only YHG=2740>be YHG=2739>detected YHG=2739>in YHG=2738>the YHG=2739>oedema YHG=2738>fluid YHG=2739>about YHG=2739>25-29 " XBD=2613 XBD=1594 XBD=1814 XBD=1912 XBD=2019 XBD=2222 XBD=2296 XHG=1410 XHG=1604 XHG=1822 XHG=1921 XHG=2030 XHG=2230 XHG=2307 YBD=2780 YBD=2770 YBD=2778 YBD=2771 YBD=2779 YHG=2742>infecting YHG=2741>organisms YHG=2740>that YHG=2741>have YHG=2741>produced YHG=2741>the YHG=2742>hyaluronidase. XBD=273 XHG=897 XBD=395 XBD=619 XBD=758 XBD=878 XBD=974 XBD=1095 XBD=1211 XBD=1350 XHG=169 XHG=300 XHG=423 XHG=678 XHG=784 XHG=1000 XHG=1122 XHG=1231 YBD=2808 YBD=2816 YHG=2777>hours YHG=2778>after YHG=2778>infection. YHG=2778>The YHG=2778>titre YHG=2787>was YHG=2778>then YHG=2779>only YHG=2778>about " XBD=2612 XBD=1510 XBD=1602 XBD=1738 XBD=1893 XBD=1962 XBD=2069 XBD=2371 XBD=2442 XBD=2515 XHG=1462 XHG=1536 XHG=1634 XHG=1767 XHG=1924 XHG=1991 XHG=2097 XHG=2399 XHG=2471 XHG=2543 YBD=2812 YBD=2809 YBD=2817 YBD=2811 YBD=2810 YHG=2782>In YHG=2780>early YHG=2779>stages YHG=2780>of YHG=2788>any YHG=2780>investigation YHG=2790>as YHG=2782>to YHG=2781>the XBD=188 XHG=800 XBD=296 XBD=385 XBD=476 XBD=543 XBD=674 XBD=776 XBD=867 XBD=1033 XBD=1126 XBD=1246 XBD=1350 XHG=173 XHG=213 XHG=320 XHG=411 XHG=503 XHG=570 XHG=727 XHG=893YBD=2847 XHG=1058 XHG=1152 XHG=1272 YBD=2845 YBD=2846 YBD=2855 YBD=2848 YBD=2847 YHG=2817>1 YHG=2817>unit YHG=2826>per YHG=2817>ml. YHG=2817>of YHG=2817>fluid. YHG=2817>In YHG=2818>muscle YHG=2818>the YHG=2818>titre YHG=2827>was " XBD=2616 XBD=1648 XBD=1708 XBD=1833 XBD=2031 XBD=2094 XBD=2179 XBD=2394 XBD=2455 XHG=1412 XHG=1668 XHG=1727 XHG=1854 XHG=2049 XHG=2113 XHG=2202 XHG=2416 XHG=2474 YBD=2848YHG=2817>the YBD=2849 YBD=2858 YBD=2850 YHG=2820>application YHG=2819>of YHG=2819>these YHG=2818>methods YHG=2818>to YHG=2819>the YHG=2820>diagnosis YHG=2820>of YHG=2820>wound " XBD=1350 XBD=364 XBD=530 XBD=644 XBD=869 XBD=1047 XBD=1196 XHG=170 XHG=399 XHG=565 XHG=903 XHG=1084 XHG=1231 YBD=2885 YBD=2892 YBD=2887 YBD=2893 YBD=2892 YBD=2893 YBD=2887 YHG=2856>somewhat YHG=2856>higher YHG=2857>and YHG=2857>appeared YHG=2857>earlier, YHG=2857>being YHG=2857>about XBD=1595XHG=679 XBD=1665 XBD=1780 XBD=1840 XBD=1903 XBD=2005 XBD=2064 XBD=2218 XBD=2385 XBD=2615 XHG=1411 XHG=1609 XHG=1683 XHG=1796 XHG=1857 XHG=1920 XHG=2021 XHG=2077 XHG=2233 XHG=2403 YBD=2895 YBD=2888 YBD=2889 YBD=2896 YHG=2860>infection YHG=2868>we YHG=2859>hope YHG=2858>be YHG=2858>able YHG=2858>to YHG=2859>supply YHG=2859>limited YHG=2859>quantities " XBD=1350 XBD=233 XBD=360 XBD=448 XBD=640 XBD=766 XBD=837 XBD=977 XBD=1120 XBD=1188 XBD=1282 XHG=171 XHG=256 XHG=383 XHG=473 XHG=666 XHG=794 XHG=1000 XHG=1144 XHG=1214 XHG=1308 YBD=2923 YBD=2924 YBD=2931 YBD=2932 YBD=2925 YBD=2932 YBD=2925 YBD=2924 YHG=2892>2-3 YHG=2894>units YHG=2904>per YHG=2904>gramme YHG=2895>after YHG=2896>18 YHG=2896>hours YHG=2896>rising YHG=2896>to YHG=2896>6-7 YHG=2895>at XBD=1453XHG=863 XBD=1567 XBD=1695 XBD=2264 XBD=2391 XBD=2616 XHG=1414 XHG=1499 XHG=1615 XHG=1743 XHG=2312 XHG=2438 YBD=2927 YBD=2926 YBD=2934 YBD=2928 YBD=2935 YHG=2898>of YHG=2898>the YHG=2897>test YHG=2896>substances-hyaluronate YHG=2898>and YHG=2897>egg-yolk " XBD=288 XBD=442 XBD=588 XBD=769 XBD=857 XBD=1116 XBD=1267 XHG=171 XHG=335 XHG=480 XHG=627 XHG=895 XHG=1157 XHG=1305 YBD=2962 YBD=2963 YBD=2964 YBD=2964 YBD=2964 YHG=2931>death. YHG=2932>With YHG=2933>other YHG=2934>strains YHG=2944>no YHG=2934>lecithinase YHG=2934>could YHG=2934>be " XBD=1351 XBD=1601XHG=807 XBD=1931 XBD=2021 XBD=2221 XBD=2285 XBD=2511 XBD=2616 XHG=1416 XHG=1626 XHG=1954 XHG=2046 XHG=2246 XHG=2309 XHG=2533 YBD=2972 YBD=2966 YBD=2974 YBD=2965 YHG=2936>(lecithin) YHG=2935>emulsion-but YHG=2935>the YHG=2936>methods YHG=2936>of YHG=2936>preparing YHG=2936>this " XBD=2613 XBD=1589 XBD=1676 XBD=1879 XBD=1989 XBD=2055 XBD=2179 XBD=2303 XBD=2414 XBD=2502 XHG=1413 XHG=1612 XHG=1701 XHG=1901 XHG=2014 XHG=2079 XHG=2192 XHG=2328 XHG=2439 XHG=2528 YBD=3005 YBD=3006 YBD=3004 YHG=2974>material YHG=2974>included YHG=2975>here YHG=2984>so YHG=2976>that. YHG=2976>those YHG=2976>who YHG=2984>are YHG=2975>able XBD=334 XHG=495 XBD=389 XBD=481 XBD=615 XHG=171 XHG=347 XHG=404 YBD=3001 YBD=3009 YHG=2969>detected YHG=2971>at YHG=2981>any YHG=2973>stage. " XBD=1504 XBD=1680 XBD=1798 XBD=1902 XBD=2104 XHG=1413 XHG=1523 XHG=1698 XHG=1816 XHG=1918 YBD=3051 YBD=3042 YBD=3043 YBD=3045 YHG=3024>may YHG=3022>prepare YHG=3013>their YHG=3022>own YHG=3013>material. " XBD=1200 XBD=924 XHG=949 XBD=993 XHG=313 XHG=1019 YBD=3071 YBD=3070 YBD=3070 YHG=3045>HYALURONIDASE-PRODUCTION YHG=3048>IN YHG=3047>CULTURE " XBD=2614 XBD=1692 XBD=1767 XBD=2089 XBD=2191 XBD=2289 XHG=1415 XHG=1718 XHG=1790 XHG=2113 XHG=2213 XHG=2314 XHG=2386 YBD=3105 YBD=3108 YBD=3107 YBD=3106 YBD=3117 YHG=3082>PREPARATION YHG=3083>OF YHG=3083>HYALURONATE YHG=3084>FOR YHG=3084>USE YHG=3083>AS YHG=3083>SUBSTRATE " XBD=2361 XBD=249 XHG=634 XBD=333 XBD=552 XBD=612 XBD=743 XBD=940 XBD=1004 XBD=1092 XBD=1218 XBD=1352 XHG=766 XHG=217 XHG=269 XHG=358 XHG=574 XHG=962YBD=3132 XHG=1025 XHG=1107 XHG=1243 YBD=3120 YBD=3121 YBD=3124 YBD=3123 YBD=3123 YBD=3123YHG=3103>enzymes YBD=3130 YBD=3124 YHG=3091>If YHG=3091>the YHG=3092>detection YHG=3093>of YHG=3093>these YHG=3094>in YHG=3093>the YHG=3093>body YHG=3093>fluids

" " "

XBD=2618 XBD=1566 XBD=1772 XBD=1900 XBD=1986 XBD=2185 XBD=2251 XBD=2350 XBD=2419 XBD=2551 XHG=1457 XHG=1592 XHG=1800 XHG=1922 XHG=2015 XHG=2212 XHG=2278 XHG=2379 XHG=2444 XHG=2580 YBD=3159 YBD=3167 YBD=3158 YBD=3157 YHG=3129>Fresh YHG=3128>umbilical YHG=3130>cords YHG=3139>-are YHG=3130>squeezed YHG=3139>as YHG=3128>free YHG=3128>of YHG=3129>blood YHG=3137>as XBD=200 XHG=909 XBD=256 XBD=319 XBD=379 XBD=474 XBD=709 XBD=838 XBD=893 XBD=942 XBD=1159 XBD=1221 XBD=1352 XHG=171 XHG=216 XHG=276 XHG=341 XHG=397 XHG=495 XHG=728 XHG=858 XHG=962YBD=3162 XHG=1178 XHG=1242 YBD=3157 YBD=3159 YBD=3160 YBD=3168 YBD=3169 YBD=3169 YBD=3162YHG=3133>is YHG=3126>is YHG=3129>to YHG=3130>be YHG=3131>of YHG=3140>any YHG=3131>diagnostic YHG=3133>value YHG=3132>it YHG=3141>necessary YHG=3132>to YHG=3133>know XBD=1563XHG=924 XBD=1652 XBD=1784 XBD=1836 XBD=1914 XBD=2048 XBD=2193 XBD=2268 XBD=2403 XBD=2456 XBD=2619 XHG=1413 XHG=1580 XHG=1667 XHG=1799 XHG=1852 XHG=1930 XHG=2096 XHG=2210 XHG=2284 XHG=2418 XHG=2473 YBD=3205 YBD=3197 YBD=3206 YBD=3198 YBD=3204 YBD=3195 YBD=3196 YHG=3168>possible YHG=3168>and YHG=3168>rinsed YHG=3172>tap YHG=3171>water. YHG=3169>They YHG=3177>are YHG=3168>stored YHG=3168>in YHG=3170>acetone XBD=252 XBD=366 XBD=421 XBD=547 XBD=777 XBD=909 XBD=967 XBD=1101 XBD=1275 XBD=1353 XHG=171 XHG=267 XHG=383 XHG=436 XHG=566 XHG=792 XHG=984YBD=3200 XHG=1117 XHG=1293 YBD=3197 YBD=3198 YBD=3199 YBD=3207 YBD=3208 YBD=3200 YBD=3201YHG=3171>to YHG=3166>that YHG=3168>most YHG=3170>of YHG=3169>those YHG=3171>organisms YHG=3171>likely YHG=3171>infect YHG=3171>wounds YHG=3171>do, XBD=1505XHG=859 XBD=1705 XBD=1792 XBD=2021 XBD=2243 XHG=1413 XHG=1520 XHG=1721 XHG=1807 XHG=2036 YBD=3235 YBD=3236 YBD=3245 YBD=3244 YHG=3207>until YHG=3207>collection YHG=3208>and YHG=3208>subsequent YHG=3208>processing. XBD=210 XBD=311 XBD=489 XBD=814 XBD=913 XBD=1012 XBD=1104 XBD=1353 XHG=172 XHG=223 XHG=329 XHG=509 XHG=930 XHG=1030 XHG=1120 YBD=3234 YBD=3243 YBD=3244 YBD=3246 YBD=3239 YBD=3239 YBD=3239 YBD=3246 YHG=3204>in YHG=3206>fact, YHG=3201>produce YHG=3209>hyaluronidase. YHG=3210>To YHG=3209>this YHG=3210>end YHG=3210>representa-

" XBD=2620 XBD=1532 XBD=1729 XBD=1979 XBD=2109 XBD=2198 XBD=2368 XBD=2566 XHG=1458 XHG=1561 XHG=1760 XHG=2009 XHG=2140 XHG=2229 XHG=2400 XHG=2599 YBD=3282 YBD=3275 YBD=3272 YBD=3281 YBD=3273 YHG=3246>The YHG=3246>partially YHG=3247>dehydrated YHG=3246>cords YHG=3254>are YHG=3245>minced YHG=3246>(through YHG=3255>a XBD=244 XHG=729 XBD=407 XBD=473 XBD=566 XBD=701 XBD=835 XBD=1356 XHG=147 XHG=270 XHG=434 XHG=499 XHG=594 XHG=863YBD=3277 YBD=3274 YBD=3279 YBD=3284 YHG=3243>tive YHG=3245>strains YHG=3247>of YHG=3247>the YHG=3248>three YHG=3248>main YHG=3248>gas-gangrene-producing XBD=1607 XBD=1767 XBD=1824 XBD=1946 XBD=2002 XBD=2178 XBD=2274 XBD=2359 XBD=2407 XBD=2467 XBD=2620 XHG=1413 XHG=1622 XHG=1786 XHG=1840 XHG=1963 XHG=2018 XHG=2225 XHG=2294 XHG=2375 XHG=2427 XHG=2479 YBD=3314 YBD=3320 YBD=3311 YBD=3319 YBD=3312 YBD=3313 YHG=3284>househdld YHG=3285>mincer) YHG=3295>as YHG=3285>finely YHG=3293>as YHG=3284>possible. YHG=3284>To YHG=3284>500 YHG=3292>g. YHG=3284>of YHG=3285>minced |>" " XBD=303 XBD=421 XBD=663 XBD=731 XBD=926 XBD=1174 XBD=1354 XBD=1262 XHG=173 XHG=328 XHG=451 XHG=690 XHG=757 XHG=953 XHG=1289 XHG=1202 YBD=3319 YBD=3315 YBD=3314 YBD=3316 YBD=3322 YBD=3322 YBD=3314 YHG=3283>species YHG=3293>were YHG=3286>cultivated YHG=3286>in YHG=3286>medium YHG=3286>containing YHG=3294>per YHG=3285>0*5 XBD=264 XHG=908 XBD=499 XBD=613 XBD=744 XBD=884 XBD=941 XBD=1098 XBD=1163 XBD=1291 XBD=1353 XHG=174 XHG=282 XHG=516 XHG=665 XHG=766 XHG=967YBD=3353 XHG=1121 XHG=1188 XHG=1310 YBD=3359 YBD=3354 YBD=3354 YBD=3354 YHG=3323>cent. YHG=3323>hyaluronic YHG=3324>acid. YHG=3324>The YHG=3324>result YHG=3324>is YHG=3325>shown YHG=3324>in YHG=3324>table YHG=3331>II. " XBD=2618 XBD=1574 XBD=1632 XBD=1779 XBD=1826 XBD=1936 XBD=2119 XBD=2263 XBD=2400 XBD=2550 XHG=1413 XHG=1603 XHG=1658 XHG=1811 XHG=1858 XHG=1966 XHG=2146 XHG=2318 XHG=2429 XHG=2579 YBD=3353 YBD=3352 YBD=3350 YBD=3360 YBD=3351 YHG=3323>material YHG=3323>is YHG=3323>added YHG=3325>1 YHG=3324>litre YHG=3322>distilled YHG=3324>water. YHG=3322>Add YHG=3323>CI-IC13 YHG=3327>to " XBD=2620 XBD=1571 XBD=1670 XBD=1789 XBD=1853 XBD=1956 XBD=2068 XBD=2288 XBD=2465 XHG=1414 XHG=1598 XHG=1697 XHG=1815 XHG=1879 XHG=1982 XHG=2095 XHG=2314 XHG=2516 YBD=3389 YBD=3397 YBD=3390 YHG=3364>saturate YHG=3362>and YHG=3365>store YHG=3363>in YHG=3361>cold YHG=3362>with YHG=3361>occasional YHG=3362>stirring. YHG=3361>After XBD=254 XHG=801 XBD=352 XBD=422 XBD=539 XBD=653 XBD=776 XBD=935 XBD=1004 XBD=1087 XBD=1254 XBD=1355 XHG=219 XHG=277 XHG=376 XHG=449 XHG=563 XHG=679 XHG=960YBD=3398 XHG=1033 XHG=1115 XHG=1276 YBD=3390 YBD=3393 YBD=3391 YBD=3392 YBD=3392 YBD=3393 YBD=3392 YBD=3391 YHG=3360>It YHG=3362>will YHG=3363>be YHG=3372>seen YHG=3362>that YHG=3362>with YHG=3363>regard YHG=3363>to YHG=3362>Cl. YHG=3362>welchii XBD=1457XHG=793 XBD=1578 XBD=1655 XBD=1766 XBD=1810 XBD=2001 XBD=2174 XBD=2216 XBD=2333 XBD=2393 XBD=2503 XBD=2620 XHG=1415 XHG=1472 XHG=1593 XHG=1675 XHG=1782 XHG=1832 XHG=2022 XHG=2195 XHG=2236 XHG=2354 XHG=2410 XHG=2523 YBD=3436 YBD=3434 YBD=3426 YBD=3435 YBD=3428 YHG=3400>24 YHG=3401>hours YHG=3401>the YHG=3401>juice YHG=3401>is YHG=3400>squeezed YHG=3399>through YHG=3408>a YHG=3400>piece YHG=3400>of YHG=3401>good YHG=3401>cloth " XBD=1353 XBD=223 XBD=419 XBD=509 XBD=707 XBD=764 XBD=897 XBD=1180 XBD=1281 XHG=175 XHG=250 XHG=443 XHG=536 XHG=729 XHG=923 XHG=1235 XHG=1312 YBD=3438 YBD=3430 YBD=3437 YBD=3437 YBD=3430 YBD=3429 YHG=3398>Cl. YHG=3400>septicum YHG=3401>the YHG=3400>position YHG=3401>is YHG=3401>quite YHG=3401>satisfactory. YHG=3400>Of YHG=3400>12 XBD=1506XHG=695 XBD=1620 XBD=1707 XBD=1756 XBD=1831 XBD=1956 XBD=1992 XBD=2139 XBD=2171 XBD=2264 XBD=2318 XBD=2435 XBD=2478 XBD=2619 XHG=1415 XHG=1520 XHG=1635 XHG=1720 XHG=1771 XHG=1845 XHG=1970 XHG=2004 XHG=2156 XHG=2187 XHG=2278 XHG=2327 XHG=2449 XHG=2492 YBD=3473 YBD=3474 YBD=3465 YBD=3466 YHG=3440>(e.g., YHG=3439>twill) YHG=3440>and YHG=3442>to YHG=3440>the YHG=3439>mince YHG=3447>a YHG=3438>further YHG=3439>1 YHG=3438>litre YHG=3438>of YHG=3441>water YHG=3439>is YHG=3439>added. " XBD=1355 XBD=676 XBD=832 XBD=1002 XBD=1068 XBD=1182 XHG=145 XHG=850 XHG=1026 XHG=1087 XHG=1202 YBD=3477 YBD=3469 YBD=3474 YBD=3468 YBD=3467 YHG=3437>hyaluronidase-producing YHG=3439>welchii YHG=3439>strains, YHG=3439>10 YHG=3447>were YHG=3438>derived ,

" " " " "

358

XBD=173 XBD=223 XBD=409 XBD=622 XBD=740 XBD=835 XBD=926 XBD=1072 XBD=1294 XHG=90 XHG=193 XHG=242 XHG=429 XHG=667 XHG=758 XHG=944YBD=209 XHG=1091 YBD=210 YBD=220 YBD=214 YBD=213 YBD=214 YHG=181>This YHG=181>is YHG=181>similarly YHG=184>extracted. YHG=185>The YHG=185>first YHG=185>and YHG=186>second YHG=185>squeezings XBD=2522XHG=854 XBD=1549 XBD=1651 XBD=1732 XBD=1869 XBD=2015 XBD=2080 XBD=2191 XBD=2235 XBD=2396 XHG=1353 XHG=1571 XHG=1673 XHG=1754 XHG=1890 XHG=2036 XHG=2100 XHG=2213 XHG=2255 XHG=2414 YBD=223 YBD=214 YBD=216 YBD=215 YBD=222 YBD=219 YHG=185>important YHG=186>that YHG=186>the YHG=196>serum YHG=188>should YHG=188>be YHG=188>from YHG=197>a YHG=188>healthy YHG=189>animal XBD=1467XHG=882 XBD=1549 XBD=1632 XBD=1738 XBD=1965 XBD=2069 XBD=2159 XBD=2319 XBD=2413 XBD=2521 XHG=1352 XHG=1485 XHG=1567 XHG=1651 XHG=1757 XHG=1982 XHG=2088 XHG=2178 XHG=2337 XHG=2429 YBD=254 YBD=253 YBD=261 YHG=224>which YHG=225>has YHG=228>not YHG=225>been YHG=226>immunised YHG=226>with YHG=235>any YHG=225>antigen YHG=226>that YHG=227>might XBD=150 XBD=290 XBD=376 XBD=609 XHG=91 XHG=164 XHG=305 XHG=390 YBD=259 YBD=252 YBD=261 YHG=230>are YHG=221>pooled YHG=223>and YHG=223>centrifuged XBD=814 XBD=865 XBD=968 XBD=1104 XBD=1154 XBD=1294 XHG=693 XHG=827 XHG=978YBD=250 XHG=1115 XHG=1166 YBD=262 YBD=253 YBD=261 YBD=252 for YHG=224>2 YHG=226>at YHG=225>3000 YHG=233>r.p.m., YHG=233>or YHG=224>passed hour XBD=1497 XBD=1792 XBD=1893 XBD=2002 XBD=2060 XBD=2215 XBD=2288 XBD=2428 XBD=2523 XHG=1352 XHG=1525 XHG=1821 XHG=1922 XHG=2031 XHG=2090 XHG=2245 XHG=2316 XHG=2452 YBD=299 YBD=291 YBD=292 YHG=264>contain YHG=263>hyaluronidase YHG=265>and YHG=264>that YHG=265>it YHG=264>should YHG=264>be YHG=263>stored YHG=265>with XBD=244 XHG=901 XBD=327 XBD=511 XBD=853 XBD=996 XBD=1046 XBD=1183 XBD=1293 XHG=90 XHG=264 XHG=348 XHG=532 XHG=1017 XHG=1068 XHG=1203 YBD=299 YBD=292 YBD=301 YBD=299 YHG=262>through YHG=263>the YHG=262>Sharples YHG=264>super-centrifuge. YHG=263>(This YHG=263>is YHG=263>better YHG=264>than XBD=241 XHG=812 XBD=378 XBD=431 XBD=477 XBD=587 XBD=694 XBD=789 XBD=985 XBD=1172 XBD=1292 XBD=1484 XBD=1739 XBD=1858 XBD=1995 XBD=2099 XBD=2163 XBD=2271 XBD=2400 XBD=2520 XHG=89 XHG=263 XHG=401 XHG=456 XHG=500 XHG=611 XHG=718 XHG=1007 XHG=1219 XHG=1351 XHG=1508 XHG=1787 XHG=1880 XHG=2008 XHG=2119 XHG=2184 XHG=2293 XHG=2420 YBD=338 YBD=332 YBD=339 YBD=333 YBD=331 YBD=331 YBD=339 YBD=330 YBD=338 YBD=329 YBD=337 YHG=301>filtering YHG=302>which YHG=304>is YHG=313>a YHG=313>very YHG=304>slow YHG=303>and YHG=302>laborious YHG=304>process.) YHG=302>The YHG=302>aseptic YHG=303>precautions. YHG=303>The YHG=312>serum YHG=312>may YHG=303>be YHG=302>kept YHG=301>under YHG=301>toluol, XBD=322 XHG=837 XBD=447 XBD=590 XBD=741 XBD=811 XBD=964 XBD=1081 XBD=1180 XBD=1292 XHG=90 XHG=349 XHG=474 XHG=615 XHG=768 XHG=990 XHG=1108 XHG=1205 YBD=378 YBD=378 YBD=372 YBD=371 YBD=370 YBD=369 YBD=370 YHG=344>supernatant YHG=342>fluid, YHG=343>which YHG=342>should YHG=342>be YHG=341>almost YHG=341>clear YHG=341>and YHG=350>very XBD=1391 XBD=1620 XBD=1723 XBD=1787 XBD=1922 XBD=1979 XBD=2022 XBD=2286 XHG=1352 XHG=1411 XHG=1640 XHG=1743 XHG=1807 XHG=1942 XHG=2000 XHG=2040 YBD=377 YBD=370 YBD=369 YBD=378 YHG=351>or YHG=340>chloroform YHG=350>may YHG=342>be YHG=342>added YHG=350>as YHG=351>a YHG=340>preservative.

" XBD=2521 XBD=1622 XBD=1677 XBD=1987 XBD=2090 XBD=2175 XBD=2241 XBD=2298 XBD=2376 XBD=2456 XHG=1395 XHG=1639 XHG=1691 XHG=2005 XHG=2108 XHG=2194 XHG=2262 XHG=2317 XHG=2392 XHG=2466 YBD=417 YBD=419 YBD=410 YBD=408 YBD=416 YBD=415 YBD=405 YBD=406 YHG=380>Preparation YHG=382>of YHG=380>mixture.-Each YHG=380>tube YHG=382>put YHG=387>up YHG=379>in YHG=379>the YHG=379>test YHG=378>will XBD=239 XHG=831 XBD=287 XBD=471 XBD=526 XBD=607 XBD=712 XBD=814 XBD=946 XHG=92 XHG=257 XHG=305 XHG=488 XHG=544 XHG=625 XHG=728 YBD=417 YBD=411 YBD=419 YBD=420 YBD=410 YBD=409 YHG=381>viscous, YHG=382>is YHG=382>adjusted YHG=385>to YHG=381>pH YHG=382>9-10 YHG=380>with YHG=379>KOH. " XBD=2521 XBD=1487 XBD=1538 XBD=1631 XBD=1750 XBD=1941 XBD=2111 XBD=2172 XBD=2307 XBD=2383 XBD=2447 XHG=1350 XHG=1523 XHG=1571 XHG=1678 XHG=1781 XHG=1972 XHG=2144 XHG=2203 XHG=2337 XHG=2414 XHG=2473 YBD=448YHG=419>methylated YBD=457 YBD=447 YBD=446 YBD=454 YBD=444 YBD=445 YHG=420>require YHG=421>1 YHG=420>ml. YHG=420>The YHG=419>required YHG=419>volume YHG=419>is YHG=417>made YHG=425>up YHG=416>in YHG=416>the XBD=304 XHG=706 XBD=453 XBD=521 XBD=693 XBD=925 XBD=1052 XBD=1095 XBD=1292 XHG=133 XHG=317 XHG=469 XHG=533 XHG=937YBD=455 XHG=1066 XHG=1108 YBD=451 YBD=458 YBD=450 YBD=456 YHG=421>Absolute YHG=422>alcohol YHG=423>(or YHG=420>absolute YHG=418>spirit) YHG=420>is YHG=419>saturated " XBD=1526 XBD=1761 XHG=1347 XHG=1541 XHG=1777 YBD=495 YBD=496 YBD=488 YHG=459>following YHG=458>proportions YHG=471>:|>"XBD=1831 XBD=176 XBD=401 XBD=593 XBD=867 XBD=952 XBD=1045 XBD=1171 XBD=1291 XBD=682XHG=900 XHG=91 XHG=206 XHG=432 XHG=712 XHG=981 XHG=1075 XHG=1201 XHG=641 YBD=489 YBD=499 YBD=493 YBD=486 YBD=494 YBD=486 YBD=489 YBD=487 YHG=461>with YHG=462>potassium YHG=463>acetate; YHG=458>volumes YHG=460>(or YHG=461>not YHG=459>Ii YHG=468>more YHG=459>than " XBD=1589 XBD=1659 XBD=1884 XHG=1554 XHG=1605 XHG=1674 XHG=1897 YBD=543 YBD=539 YHG=514>10 YHG=514>ml. YHG=513>hyaluronate YHG=513>solution. "|>"XBD=2044 XBD=323 XBD=383 XBD=467 XBD=622 XBD=668 XBD=802 XBD=857 XBD=936 XBD=1185 XBD=1291 XBD=134XHG=875 XHG=152 XHG=343 XHG=397 XHG=486 XHG=638 XHG=686 XHG=819 XHG=955 XHG=1202 XHG=94YBD=526 YBD=537 YBD=529 YBD=528 YBD=525 YBD=545 YBD=535 YBD=526YHG=498>the YHG=501>volumes) YHG=500>of YHG=500>this YHG=496>alcohol YHG=498>is YHG=497>added YHG=499>to YHG=501>It YHG=500>supernatant YHG=497>fluid

"|>"XBD=1290 XBD=176 XBD=364 XBD=718 XBD=817 XBD=887 XBD=975 XBD=493XHG=915 XHG=90 XHG=207 XHG=523 XHG=748 XHG=847 XHG=1006 XHG=413 YBD=568 YBD=575 YBD=574 YBD=566 YBD=564 YBD=565 YBD=566 YBD=574 YHG=539>with YHG=540>shaking. YHG=537>potassium YHG=536>salt YHG=536>of YHG=537>the YHG=530>’The YHG=537>polysaccharide " XBD=2211 XBD=2331 XHG=2224 XBD=1590 XBD=1659 XBD=1709 XBD=1742 XBD=1796 XBD=1846 XBD=1998 XBD=2046 XBD=2164 XHG=1554 YBD=573 XHG=1605 XHG=1675 XHG=1722 XHG=1757 XHG=1811 XHG=1862 XHG=2011 XHG=2057 XHG=2176 YBD=575 YHG=541>saline. YBD=574 YHG=551>10: YHG=550>ml. YHG=550>of YHG=556>a YHG=551>1 YHG=550>10 YHG=549>dilution YHG=548>of YHG=555>serum YHG=549>in " XBD=205 XHG=615 XBD=292 XBD=356 XBD=529 XBD=591 XBD=636 XBD=916 XBD=1031 XBD=1143 XBD=1236 XBD=1290 XHG=659 XHG=89 XHG=229 XHG=316 XHG=376 XHG=552 XHG=941 XHG=1069 XHG=1166 XHG=1259 YBD=608 YBD=608 YBD=606 YBD=604 YBD=605 YBD=608 YBD=605 YBD=604 YHG=588>comes YHG=581>out YHG=578>of YHG=577>solution YHG=585>as YHG=585>a YHG=575>characteristic YHG=576>clot. YHG=577>The YHG=577>clot YHG=576>is " XBD=1993 XBD=1589 XBD=1658 XBD=1708 XBD=1859 XHG=1552 XHG=1604 XHG=1675 XHG=1720 XHG=1871 YBD=611 YBD=614 YHG=586>20 YHG=586>ml. YHG=586>of YHG=585>distilled YHG=586>water..

"|>" XBD=344 XBD=389 XBD=576 XBD=720 XBD=815 XBD=865 XBD=1022 XBD=1093 XBD=1204 XBD=1290 XBD=274XHG=837 XHG=298 XHG=368 XHG=412 XHG=599 XHG=743 XHG=890 XHG=1045 XHG=1116 XHG=1227 XHG=89YBD=643 YBD=645 YBD=644 YBD=655 YBD=644 YBD=653 YBD=645 YBD=643 YHG=625>on YHG=625>a YHG=616>Buchner YHG=615>funnel YHG=615>and YHG=615>is YHG=617>separated YHG=616>broken YHG=625>up YHG=615>with YHG=615>the " XBD=1432 XBD=1756 XBD=1924 XBD=2010 XBD=2113 XBD=2187 XBD=2226 XBD=2308 XBD=2409 XBD=2458 XHG=1349 XHG=1441 XHG=1772 XHG=1941 XHG=2026 XHG=2132 XHG=2204 XHG=2241 XHG=2325 XHG=2426 XHG=2473 YBD=670 YBD=669 YBD=676 YBD=668 YBD=674 YBD=666 YBD=667 YHG=640>This YHG=640>,serum.substrate YHG=639>mixture YHG=640>will YHG=640>keep YHG=640>for YHG=649>a YHG=638>few YHG=637>days YHG=638>in YHG=638>the "" XBD=2521 XBD=216 XBD=304 XBD=462 XBD=565 XBD=623 XBD=740 XBD=844 XBD=947 XBD=1099 XBD=1171 XBD=1287 XHG=89 XHG=232 XHG=321 XHG=479 XHG=584 XHG=639 XHG=756 XHG=860 XHG=964 XHG=1119 XHG=1188 YBD=693 YBD=684 YBD=682 YBD=683 YBD=692 YBD=684 YBD=691 YBD=683 YHG=657>fingers YHG=656>and YHG=654>washed YHG=664>once YHG=663>or YHG=653>twice YHG=655>with YHG=664>pure YHG=656>alcohol YHG=657>(or YHG=655>absoXBD=1578 XBD=1667 XBD=1719 XBD=1765 XBD=1814 XBD=1898 XBD=2005 XBD=2072 XBD=2154 XBD=2246 XBD=2306 XBD=2522 XHG=1347 XHG=1601 XHG=1688 XHG=1735 XHG=1785 XHG=1834 XHG=1919 XHG=2026 XHG=2093 XHG=2175 XHG=2267 XHG=2322 YBD=708 YBD=707 YBD=706 YBD=715 YHG=679>refrigerator, YHG=679>but YHG=679>if YHG=679>it YHG=679>is YHG=682>not YHG=680>used YHG=687>on YHG=679>the YHG=679>day YHG=677>of YHG=677>preparation "" XBD=1290 XBD=163 XBD=404 XBD=547 XBD=626 XBD=680 XBD=827 XBD=983 XBD=1085 XBD=1201 XHG=87 XHG=185 XHG=420 XHG=589 XHG=651 XHG=706 XHG=843 XHG=999 XHG=1102 XHG=1217 YBD=724 YBD=730 YBD=729 YBD=722 YBD=723 YBD=722 YHG=696>lute YHG=689>methylated YHG=693>spirit). YHG=694>It YHG=693>is YHG=693>finally YHG=695>washed YHG=695>with YHG=695>ether XBD=1378 XHG=2386 XBD=1427 XBD=1528 XBD=1585 XBD=1707 XBD=1805 XBD=1905 XBD=1952 XBD=2041 XBD=2132 XBD=2221 XBD=2288 XBD=2366 XBD=2523 XHG=1346 YBD=746 XHG=1397 XHG=1445 XHG=1547 XHG=1604 XHG=1726 XHG=1824 XHG=1924 XHG=1971 XHG=2061 XHG=2151 XHG=2242 XHG=2307 YBD=748 YHG=693>and YBD=747 YBD=745 YHG=719>is YHG=719>wise YHG=721>to YHG=719>make YHG=727>sure YHG=719>that YHG=719>it YHG=719>will YHG=718>still YHG=718>clot YHG=727>on YHG=717>the YHG=716>addition |>" " XBD=188 XBD=301 XBD=423 XBD=502 XBD=642 XBD=769 XBD=984 XBD=1139 XBD=1289 XBD=904 XHG=90 XHG=216 XHG=329 XHG=452 XHG=518 XHG=696 XHG=932 XHG=1012 XHG=1167 XHG=798 YBD=763 YBD=762 YBD=770 YBD=760 YBD=761 YBD=769 YBD=764 YBD=762 YBD=768 YHG=734>dried YHG=742>over YHG=732>P20r, YHG=732>iif YHG=742>vacuo. YHG=733>The YHG=735>(or YHG=733>white YHG=734>almost " XBD=2524 XBD=1389 XHG=2479 XBD=1514 XBD=1613 XBD=1719 XBD=1855 XBD=1946 XBD=2056 XBD=2278 XBD=2334 XBD=2411 XBD=2461 XHG=1349 YBD=785 XHG=1404 XHG=1535 XHG=1632 XHG=1739 XHG=1874 XHG=1964 XHG=2074 XHG=2297 XHG=2355 XHG=2430 YBD=786 YHG=733>white) YHG=757>of YHG=759>acetic YHG=758>acid YHG=758>both YHG=757>before YHG=758>and YHG=757>after YHG=757>incubation YHG=759>at YHG=755>37° YHG=757>C. YHG=756>for " XBD=248 XBD=296 XBD=385 XBD=563 XBD=654 XBD=757 XBD=820 XBD=974 XBD=1031 XBD=1073 XBD=1233 XHG=89 XHG=267 XHG=316 XHG=405 XHG=583 XHG=673 XHG=777 XHG=993 XHG=1052 XHG=1091 XHG=1252 YBD=801 YBD=800 YBD=808 YBD=801 YBD=810 YBD=800 YHG=772>material YHG=772>is YHG=771>like YHG=772>asbestos YHG=772>and YHG=781>may YHG=772>be YHG=773>ground YHG=775>to YHG=782>a YHG=772>powder YHG=771>in " XBD=1291 XBD=1392XHG=838 XBD=1493 XBD=1541 XHG=1350 XHG=1407 XHG=1535 YBD=826 YBD=827 YHG=797>20 YHG=797>min. YHG=810>t " XBD=110 XBD=261 XBD=351 XBD=512 XBD=701 XBD=863 XBD=947 XBD=998 XBD=1058 XBD=1213 XBD=1290 XHG=89 XHG=128 XHG=279 XHG=368 XHG=530 XHG=743 XHG=883 XHG=965 XHG=1018 XHG=1072 XHG=1229 YBD=839 YBD=839 YBD=849 YBD=840 YBD=841 YBD=848 YBD=849 YBD=840 YHG=821>a YHG=813>mortar YHG=811>and YHG=810>used YHG=811>required. YHG=811>About YHG=813>2-3 YHG=821>g. YHG=811>of YHG=812>powder YHG=819>are as " XBD=2164 XBD=1885 XHG=2075 XBD=1956 XBD=2051 XHG=1731 YBD=866 XHG=1908 XHG=1979 YBD=865 YHG=845>TEST YHG=844>SETTING YHG=844>UP YHG=844>THE " XBD=840 XBD=257 XHG=746 XBD=368 XBD=455 XBD=507 XBD=571 XBD=725 XHG=89 YBD=879 XHG=275 XHG=389 XHG=474 XHG=530 XHG=587 YBD=887YHG=850>obtained YBD=879 YBD=882 YBD=881 YHG=850>from YHG=851>500 YHG=859>g. YHG=850>of YHG=852>minced YHG=851>cord.

" XBD=2434 XBD=2522XHG=838 XBD=241 XBD=343 XBD=493 XBD=559 XBD=756 XBD=817 XBD=899 XBD=1145 XBD=1208 XBD=1289 XBD=1424 XBD=1625 XBD=1681 XBD=1747 XBD=1899 XBD=2200 XBD=2291 XBD=2375 XHG=134 XHG=264 XHG=366 XHG=515 XHG=583 XHG=779 XHG=921YBD=919 XHG=1168 XHG=1227 XHG=1397 XHG=1450 XHG=1644 XHG=1696 XHG=1767 XHG=1917 XHG=2221 XHG=2311 XHG=2394 XHG=2452 YBD=928 YBD=917 YBD=927 YBD=928 YBD=919 YBD=918 YBD=921 YHG=890>Great YHG=899>care YHG=889>should YHG=891>be YHG=891>exercised YHG=892>in YHG=890>the YHG=891>preparation YHG=889>of YHG=889>the YHG=891>1. YHG=889>Dilutions YHG=890>of YHG=890>the YHG=899>enzyme YHG=891>samples.-This YHG=892>test YHG=900>can YHG=889>be YHG=892>used " XBD=2522 XBD=372 XHG=455 XBD=434 XBD=605 XBD=904 XBD=1036 XBD=1144 XBD=1288 XHG=627 XHG=88 XHG=394 XHG=926 XHG=1057 XHG=1166 YBD=965 YBD=966 YBD=958 YBD=958 YBD=965 YHG=929>polysaccharide YHG=932>to YHG=933>prevent YHG=930>contamination YHG=930>either YHG=929>with YHG=937>organXBD=1387 XBD=1506 XBD=1671 XBD=1722 XBD=1819 XBD=2050 XBD=2131 XBD=2173 XBD=2216 XBD=2366 XHG=1348 XHG=1402 XHG=1518 XHG=1684 XHG=1738 XHG=1838 XHG=2066 XHG=2145 XHG=2187 XHG=2230 XHG=2379 YBD=968 YBD=964 YBD=958 YBD=966 YBD=959 YHG=931>to YHG=938>assay YHG=932>potency YHG=932>to YHG=930>20% YHG=929>differences, YHG=930>but YHG=930>it YHG=930>is YHG=930>usually YHG=938>unneces.

XBD=172 XBD=232 XBD=338 XBD=636 XBD=802 XBD=991 XBD=1137 XBD=1289 XHG=87 XHG=193 XHG=251 XHG=358 XHG=683 XHG=1011 XHG=1157 YBD=996 YBD=996 YBD=1005 YBD=997 YBD=998 YBD=1003 YHG=969>isms YHG=977>or YHG=968>with YHG=969>Water YHG=969>solutions YHG=968>hyaluronidase. YHG=968>should YHG=968>always " XBD=2467 XBD=2560XHG=822 XBD=1430 XBD=1492 XBD=1585 XBD=1715 XBD=1826 XBD=1992 XBD=2225 XBD=2309 XBD=2421 XHG=1348 XHG=1453 XHG=1515 XHG=1607 XHG=1736 XHG=1847 XHG=2014 XHG=2271 XHG=2327 XHG=2441 XHG=2484 YBD=1005 YBD=996 YBD=997 YBD=1002 YBD=998 YHG=970>to YHG=971>test YHG=968>closer YHG=968>than YHG=969>twofold YHG=969>differences. YHG=970>there YHG=969>is YHG=979>no YHG=977>sary YHG=968>If XBD=132 XHG=820 XBD=337 XBD=445 XBD=683 XBD=801 XBD=906 XBD=995 XBD=1289 XHG=929 XHG=88 XHG=156 XHG=359 XHG=469 XHG=706 XHG=1017 YBD=1036 YBD=1036 YBD=1037 YBD=1045 YHG=1008>be YHG=1008>saturated YHG=1007>CHCIs YHG=1008>work YHG=1008>with YHG=1009>dry YHG=1007>hyaluronidase .and " XBD=2523 XBD=1552 XBD=1612 XBD=1690 XBD=1951 XBD=2124 XBD=2184 XBD=2262 XBD=2411 XHG=1348 XHG=1572 XHG=1627 XHG=1709 XHG=1970 XHG=2144 XHG=2200 XHG=2282 XHG=2429 YBD=1044 YBD=1036 YBD=1045 YBD=1037 YBD=1046 YBD=1038 YHG=1007>knowledge YHG=1007>of YHG=1007>the YHG=1002>approximate YHG=1011>potency YHG=1008>of YHG=1008>the YHG=1008>sample YHG=1009>under

" " " "

XBD=214 XBD=297 XBD=362 XBD=515 XBD=600 XBD=658 XBD=738 XBD=854 XBD=982 XHG=86 XHG=232 XHG=318 XHG=382 XHG=536 XHG=620 XHG=677 XHG=758 YBD=1075 YBD=1075 YBD=1076 YBD=1077 YHG=1047>should YHG=1049>not YHG=1047>be YHG=1047>carried YHG=1050>out YHG=1047>in YHG=1046>the YHG=1056>same YHG=1057>room. XBD=2522XHG=874 XBD=1420 XBD=1458 XBD=1696 XBD=1872 XBD=1925 XBD=2074 XBD=2256 XBD=2354 XBD=2411 XHG=1348 XHG=1436 XHG=1473 XHG=1711 XHG=1887 XHG=1940 XHG=2090 XHG=2272 XHG=2369 XHG=2425 YBD=1075 YBD=1084 YBD=1077 YBD=1076 YBD=1085 YHG=1050>test YHG=1056>a YHG=1043>preliminary YHG=1047>titration YHG=1047>in YHG=1047>tenfold YHG=1048>dilutions YHG=1057>may YHG=1048>be YHG=1049>made, XBD=1510 XBD=1573 XBD=1612 XBD=1699 XBD=1754 XBD=1916 XBD=2142 XHG=1345 XHG=1525 XHG=1589 XHG=1629 XHG=1715 XHG=1769 XHG=1931 YBD=1123 YBD=1116 YBD=1117 YHG=1086>followed YHG=1086>by YHG=1096>a YHG=1089>test YHG=1090>at YHG=1086>twofold YHG=1087>differences. XBD=271 XHG=664 XBD=317 XBD=547 XBD=645 XBD=853 XBD=1041 XBD=1101 XBD=1285 XHG=137 XHG=292 XHG=338 XHG=567 XHG=874YBD=1132 XHG=1062 XHG=1120 YBD=1131 YBD=1124 YBD=1133 YBD=1124YHG=1093>adequate YBD=1132 YHG=1092>Where YHG=1103>a YHG=1092>centrifuge YHG=1093>and YHG=1094>supplies YHG=1094>of YHG=1093>methylXBD=1466 XBD=1705 XBD=1888 XBD=1945 XBD=2019 XBD=2188 XBD=2245 XBD=2389 XBD=2523 XHG=1392 XHG=1483 XHG=1722 XHG=1906 XHG=1959 XHG=2036 XHG=2205 XHG=2260 XHG=2405 YBD=1166 YBD=1155 YBD=1156 YBD=1157 YHG=1126>The YHG=1127>appropriate YHG=1126>dilutions YHG=1127>of YHG=1127>the YHG=1128>exudate YHG=1137>or YHG=1127>muscle YHG=1130>extract XBD=179 XHG=744 XBD=317 XBD=405 XBD=504 XBD=718 XBD=811 XBD=1029 XBD=1285 XHG=87 XHG=196 XHG=342 XHG=434 XHG=530 XHG=838 XHG=1055 YBD=1163 YBD=1171 YBD=1163 YBD=1164 YBD=1172 YBD=1165 YHG=1133>ated YHG=1133>-spirit YHG=1142>are YHG=1133>not YHG=1133>available YHG=1134>the YHG=1134>following YHG=1133>alternative

|>" " XBD=1386 XBD=1448 XBD=1580 XBD=1655 XBD=1773 XBD=1826 XBD=1864 XBD=1963 XBD=2120 XBD=2176 XBD=2320 XBD=2379 XBD=2245 XHG=1347 XHG=1403 XHG=1465 XHG=1596 XHG=1670 XHG=1789 XHG=1843 XHG=1878 XHG=1980 XHG=2137 XHG=2261 XHG=2339 XHG=2391 XHG=2190 YBD=1194 YBD=1195 YBD=1197 YBD=1195 YHG=1168>to YHG=1166>be YHG=1165>tested YHG=1175>are YHG=1166>made YHG=1167>in YHG=1176>a YHG=1167>final YHG=1166>volume YHG=1166>of YHG=1167>ml. YHG=1167>of YHG=1167>distilled YHG=1168>05 " XBD=2523 XBD=263 XBD=374 XBD=440 XBD=631 XHG=88 XHG=282 XHG=393 XHG=460 YBD=1203 YBD=1210 YBD=1203 YHG=1172>methods YHG=1181>may YHG=1172>be YHG=1173>used

XBD=157 XHG=98 YBD=1233 YHG=1217>«*.." XBD=1465 XBD=1557 XBD=1675 XBD=1730 XBD=1871 XBD=2126 XBD=2269 XBD=2317 XBD=2353 XBD=2449 XBD=2521 XHG=1346 XHG=1512 XHG=1575 XHG=1692 XHG=1749 XHG=1888 XHG=2144 XHG=2287 XHG=2332 XHG=2370 XHG=2465 YBD=1233 YBD=1242 YBD=1234 YBD=1235 YBD=1236 YHG=1207>water. YHG=1206>In YHG=1205>order YHG=1208>to YHG=1205>obtain YHG=1206>reproducible YHG=1206>results YHG=1207>if YHG=1216>a YHG=1207>final YHG=1211>test |>" " XBD=673 XBD=889 XBD=616 XHG=629 XHG=688 XHG=904 XHG=378 YBD=1263 YBD=1264 YBD=1263 YBD=1262 YHG=1242>OF YHG=1241>UMBILICAL YHG=1242>CORD YHG=1241>EXTRACTION " XBD=1000 XBD=1386 XBD=1495 XBD=1724 XBD=1778 XBD=1917 XBD=1971 XBD=2024 XBD=2231 XBD=2294 XBD=2418 XBD=2522 XHG=1347 XHG=1413 XHG=1525 XHG=1749 XHG=1803 XHG=1942 XHG=1996 XHG=2048 XHG=2256 XHG=2318 XHG=2441 YBD=1280 YBD=1273 YBD=1272 YBD=1282 YBD=1274 YBD=1276 YHG=1247>at YHG=1244>20% YHG=1245>differences YHG=1245>made, YHG=1245>it YHG=1245>is YHG=1254>necessary YHG=1248>to YHG=1245>make YHG=1247>these |>" XBD=1515 XBD=1568 XBD=1680 XBD=1760 XBD=1819 XBD=1907 XBD=1995 XBD=2049 XBD=2216 XBD=2361 XBD=2522 XBD=2287 XHG=1347 XHG=1530 XHG=1583 XHG=1696 XHG=1779 XHG=1834 XHG=1925 XHG=2011 XHG=2066 XHG=2303 XHG=2378 XHG=2233 YBD=1318 YBD=1320 YBD=1319 YBD=1313 YBD=1314 YBD=1315 YBD=1314 YHG=1284>dilutions YHG=1284>in YHG=1284>bulk, YHG=1293>say YHG=1285>10 YHG=1285>ml., YHG=1284>and YHG=1287>to YHG=1284>transfer YHG=1285>ml. YHG=1289>amounts YHG=1285>0’5 " XBD=232 XBD=399 XBD=483 XBD=782 XBD=896 XBD=972 XBD=1100 XBD=1145 XBD=1287 XHG=130 XHG=245 XHG=414 XHG=491 XHG=797 XHG=912 XHG=988 XHG=1116 XHG=1160 YBD=1319 YBD=1311 YBD=1313 YBD=1312 YBD=1313 YBD=1312 YBD=1321 YHG=1283>After YHG=1282>mincing YHG=1283>theYHG=1283>acetone-soaked YHG=1283>cords YHG=1283>the YHG=1283>mince YHG=1284>is YHG=1284>spread "

" XBD=1387 XBD=1462 XBD=1645 XBD=1700 XBD=1779 XBD=1898 XBD=1969 XHG=1347 XHG=1400 XHG=1480 XHG=1663 XHG=1717 XHG=1796 XHG=1915 XHG=1986 YBD=1352 YBD=1353 YHG=1325>of YHG=1323>the YHG=1324>dilutions YHG=1326>to YHG=1324>the YHG=1324>tubes YHG=1323>for YHG=1326>test. " XBD=2067 XBD=150 XHG=898 XBD=210 XBD=331 XBD=398 XBD=433 XBD=512 XBD=632 XBD=684 XBD=886 XBD=991 XBD=1045 XBD=1115 XBD=1284 XHG=86 XHG=163 XHG=221 XHG=343 XHG=411 XHG=445 XHG=525 XHG=646 XHG=698 XHG=1005 XHG=1054 XHG=1128 YBD=1351 YBD=1351 YBD=1360 YBD=1352 YBD=1352 YBD=1351 YBD=1353 YHG=1324>out YHG=1332>on YHG=1331>paper YHG=1321>for YHG=1332>a YHG=1323>few YHG=1322>hours YHG=1326>to YHG=1326>evaporate YHG=1326>most YHG=1323>of YHG=1323>the YHG=1326>acetone.

|>" XBD=250 XBD=290 XBD=392 XBD=454 XBD=579 XBD=628 XBD=764 XBD=823 XBD=954 XBD=1156 XBD=1286 XBD=1092 XHG=129 XHG=275 XHG=313 XHG=413 XHG=469 XHG=599 XHG=649 XHG=785 XHG=844 XHG=1116 XHG=1172 XHG=976 YBD=1390 YBD=1391 YBD=1392 YBD=1400 YHG=1362>About YHG=1363>1 YHG=1361>litre YHG=1362>of YHG=1365>water YHG=1363>is YHG=1363>added YHG=1365>to YHG=1363>about YHG=1363>of YHG=1364>mince YHG=1364>500 g. " XBD=1424 XBD=1614 XBD=1683 XBD=1946 XBD=2040 XBD=2116 XBD=2205 XBD=2522 XHG=1393 XHG=1448 XHG=1646 XHG=1711 XHG=1981 XHG=2075 XHG=2144 XHG=2237 YBD=1400 YBD=1393 YBD=1391 YBD=1392 YBD=1394 YHG=1365>2. YHG=1364>Addition YHG=1364>of YHG=1364>substrate.-1 YHG=1364>ml. YHG=1362>of YHG=1363>the YHG=1364>substrate-serum " XBD=2522 XBD=157 XHG=832 XBD=238 XBD=278 XBD=353 XBD=402 XBD=520 XBD=586 XBD=613 XBD=664 XBD=695 XBD=823 XBD=918 XBD=1124 XBD=1284 XHG=86 XHG=168 XHG=246 XHG=289 XHG=364 XHG=414 XHG=530 XHG=598 XHG=625 XHG=676 XHG=706 XHG=930YBD=1430 XHG=1134 YBD=1430 YBD=1431 YBD=1438 YBD=1432 YBD=1431 YHG=1402>and YHG=1401>this YHG=1403>is YHG=1401>left YHG=1404>to YHG=1403>stand YHG=1402>for YHG=1404>1 YHG=1412>or YHG=1403>2 YHG=1403>hours, YHG=1403>with YHG=1397>occasional YHG=1403>stirring. XBD=1500 XBD=1545 XBD=1676 XBD=1730 XBD=1832 XBD=1944 XBD=2138 XBD=2215 XBD=2376 XHG=1346 XHG=1516 XHG=1561 XHG=1692 XHG=1746 XHG=1848 XHG=1961 XHG=2154 XHG=2231 XHG=2392 YBD=1432 YBD=1437 YBD=1439 YBD=1431 YBD=1438 YBD=1441 YHG=1403>mixture YHG=1403>is YHG=1403>added YHG=1406>to YHG=1403>each YHG=1403>tube, YHG=1402>including YHG=1403>the YHG=1403>control, YHG=1405>starting XBD=121 XHG=858 XBD=170 XBD=275 XBD=463 XBD=634 XBD=691 XBD=840 XBD=944 XBD=1128 XBD=1286 XHG=85 XHG=141 XHG=189 XHG=294 XHG=482 XHG=652 XHG=711 XHG=964 XHG=1146 YBD=1470 YBD=1469 YBD=1478 YBD=1470 YBD=1479 YBD=1471 YBD=1471 YBD=1478 YHG=1442>It YHG=1442>is YHG=1441>then YHG=1442>carefully YHG=1442>brought YHG=1444>to YHG=1442>boiling YHG=1443>with YHG=1444>constant YHG=1443>stirring " XBD=2522 XBD=1438 XBD=1559 XBD=1781 XBD=1862 XBD=2020 XBD=2192 XBD=2253 XBD=2421 XHG=1345 XHG=1458 XHG=1580 XHG=1801 XHG=1882 XHG=2042 XHG=2212 XHG=2270 XHG=2463 YBD=1479 YBD=1471 YBD=1470 YBD=1480 YBD=1472 YHG=1444>from YHG=1442>those YHG=1442>containing YHG=1442>the YHG=1442>highest YHG=1442>dilution YHG=1442>of YHG=1452>enzyme. YHG=1444>The " XBD=156 XBD=288 XBD=358 XBD=438 XBD=549 XBD=654 XBD=690 XBD=791 XBD=910 XBD=1033 XBD=1132 XHG=85 XHG=172 XHG=303 XHG=372 XHG=453 XHG=565 XHG=671 XHG=706 XHG=926 XHG=1049 XHG=1148 YBD=1510 YBD=1510 YBD=1511 YBD=1510 YBD=1510 YBD=1511 YHG=1481>and YHG=1481>boiled YHG=1481>for YHG=1484>not YHG=1491>more YHG=1482>than YHG=1484>3 YHG=1483>min. YHG=1482>The YHG=1483>tissue YHG=1482>thus YHG=1482>shrinks " XBD=1285 XBD=1509XHG=837 XBD=1584 XBD=1717 XBD=1782 XBD=1972 XBD=2049 XBD=2190 XBD=2251 XBD=2295 XBD=2375 XBD=2522 XHG=1346 XHG=1527 XHG=1600 XHG=1734 XHG=1798 XHG=1988 XHG=2065 XHG=2221 XHG=2266 XHG=2313 XHG=2389 YBD=1518 YBD=1516 YBD=1510 YBD=1511 YBD=1520 YHG=1485>contents YHG=1492>are YHG=1482>mixed YHG=1483>by YHG=1482>inverting YHG=1482>the YHG=1482>tubes YHG=1482>it YHG=1482>is YHG=1492>our YHG=1484>practice ; XBD=156 XHG=900 XBD=335 XBD=411 XBD=629 XBD=734 XBD=885 XBD=971 XBD=1048 XBD=1091 XBD=1135 XBD=1285 XHG=85 XHG=171 XHG=349 XHG=425 XHG=645 XHG=773 XHG=984 XHG=1063 XHG=1106 XHG=1149 YBD=1550 YBD=1549 YBD=1557 YBD=1551 YBD=1550 YBD=1550 YHG=1521>And YHG=1520>extrudes YHG=1522>the YHG=1522>hyaluronic YHG=1522>acid. YHG=1521>While YHG=1522>still YHG=1521>hot YHG=1522>it YHG=1522>is YHG=1522>filtered " XBD=2522 XBD=1385 XBD=1503 XBD=1543 XBD=1661 XBD=1774 XBD=1833 XBD=1945 XBD=2074 XBD=2248 XBD=2327 XBD=2449 XHG=1346 XHG=1403 XHG=1522 XHG=1561 XHG=1678 XHG=1793 XHG=1847 XHG=1964 XHG=2091 XHG=2267 XHG=2344 XHG=2464 YBD=1559 YBD=1550 YBD=1558 YBD=1551 YHG=1525>to YHG=1522>place YHG=1531>a YHG=1522>small YHG=1523>piece YHG=1521>of YHG=1531>scrap YHG=1530>paper YHG=1522>between YHG=1522>the YHG=1522>finger YHG=1523>and " XBD=2522 XBD=1408 XBD=1554 XBD=1614 XBD=1691 XBD=1797 XBD=1918 XBD=2111 XBD=2170 XBD=2297 XHG=1345 XHG=1427 XHG=1574 XHG=1631 XHG=1712 XHG=1817 XHG=1938 XHG=2133 XHG=2192 XHG=2317 YBD=1597 YBD=1589 YBD=1598 YHG=1561>mouth YHG=1562>of YHG=1561>the YHG=1562>tube YHG=1561>when YHG=1560>inverting YHG=1563>to YHG=1561>avoid YHG=1561>transferring XBD=238 XHG=815 XBD=359 XBD=584 XBD=784 XBD=918 XBD=1112 XBD=1233 XBD=1286 XHG=84 XHG=266 XHG=388 XHG=614 XHG=946 XHG=1142 XHG=1264 YBD=1597 YBD=1590 YBD=1589 YBD=1597 YBD=1598 YHG=1561>through YHG=1560>glass YHG=1561>wool-the YHG=1561>filtration YHG=1561>being YHG=1561>repeated YHG=1561>until YHG=1570>a " XBD=1506 XBD=1614 XBD=1697 XBD=1800 XBD=1856 XHG=1345 XHG=1523 XHG=1632 XHG=1713 XHG=1818 XHG=1874 YBD=1629 YBD=1630 YBD=1628 YHG=1601>material YHG=1600>from YHG=1609>one YHG=1601>tube YHG=1602>to YHG=1600>another. " XBD=2029 XBD=291 XHG=850 XBD=399 XBD=569 XBD=614 XBD=809 XBD=932 XBD=1101 XBD=1147 XBD=1285 XHG=83 XHG=308 XHG=416 XHG=585 XHG=631 XHG=950 XHG=1118 XHG=1165 YBD=1637 YBD=1629 YBD=1629 YBD=1628 YBD=1629 YHG=1600>reasonably YHG=1600>clear YHG=1600>solution YHG=1600>is YHG=1600>obtained. YHG=1601>This YHG=1601>solution YHG=1600>is YHG=1600>cooled " XBD=2521 XBD=1421 XBD=1753 XBD=1852 XBD=1905 XBD=2050 XBD=2092 XBD=2279 XBD=2353 XBD=2462 XHG=1391 XHG=1446 XHG=1767 XHG=1866 XHG=1918 XHG=2064 XHG=2107 XHG=2292 XHG=2366 XHG=2475 YBD=1669 YBD=1670 YBD=1675 YBD=1667 YBD=1676 YBD=1668 YHG=1641>3. YHG=1640>Incubation.-As YHG=1648>soon YHG=1648>as YHG=1639>mixing YHG=1639>is YHG=1639>complete YHG=1640>the YHG=1639>tubes YHG=1649>are XBD=156 XHG=832 XBD=429 XBD=493 XBD=641 XBD=704 XBD=811 XBD=968 XBD=1089 XBD=1198 XHG=85 XHG=175 XHG=464 XHG=515 XHG=660 XHG=725 XHG=988 XHG=1110 YBD=1668 YBD=1678 YBD=1676 YBD=1668 YBD=1668 YHG=1640>and YHG=1640>precipitated; YHG=1640>it YHG=1640>should YHG=1640>be YHG=1649>very YHG=1640>viscous YHG=1640>when YHG=1640>cold. " XBD=1469 XBD=1523 XBD=1561 XBD=1789 XBD=1844 XBD=1922 XBD=1975 XBD=2049 XBD=2109 XBD=2209 XHG=1344 XHG=1486 XHG=1540 XHG=1577 XHG=1805 XHG=1863 XHG=1939 XHG=1994 XHG=2066 XHG=2125 YBD=1717 YBD=1707 YBD=1708 YHG=1679>placed YHG=1680>in YHG=1689>a YHG=1679>water-bath YHG=1682>at YHG=1679>37° YHG=1679>C. YHG=1678>for YHG=1680>20 YHG=1679>min. " XBD=424 XBD=538 XBD=725 XBD=786 XBD=1024 XHG=143 XHG=439 XHG=552 XHG=801 XHG=1040 YBD=1734 YBD=1740 YBD=1733 YBD=1733 YBD=1734 YHG=1712>PRECIPITATION YHG=1713>WITH YHG=1712>MINIMUM YHG=1713>OF YHG=1712>INDUSTRIAL YHG=1712>SOLVENTS " XBD=1223 XBD=1421XHG=741 XBD=1730 XBD=1816 XBD=1967 XBD=2229 XBD=2328 XBD=2383 XBD=2485 XBD=2521 XHG=1390 XHG=1448 XHG=1745 XHG=1829 XHG=1981 XHG=2244 XHG=2343 XHG=2393 XHG=2499 YBD=1755 YBD=1748 YBD=1747 YHG=1720>4. YHG=1719>Clot-production YHG=1719>and YHG=1719>reading YHG=1719>results.-The YHG=1719>rack YHG=1718>of YHG=1719>tubes YHG=1718>is "" XBD=1284 XBD=173 XBD=256 XBD=418 XBD=544 XBD=685 XBD=737 XBD=879 XBD=941 XBD=985 XBD=1092 XBD=1157 XHG=99 XHG=197 XHG=282 XHG=442 XHG=569 XHG=709 XHG=763 XHG=903 XHG=1014 XHG=1117 XHG=1178 YBD=1784 YBD=1785 YBD=1783 YBD=1782 YBD=1782 YBD=1783 YHG=1744>30 YHG=1755>ml. YHG=1755>normal YHG=1755>horse YHG=1764>serum YHG=1755>is YHG=1754>added YHG=1757>to YHG=1755>1 YHG=1754>litre YHG=1753>of YHG=1754>crude XBD=1510XHG=971 XBD=1620 XBD=1700 XBD=1808 XBD=1900 XBD=2057 XBD=2197 XBD=2266 XBD=2476 XBD=2522 XHG=1343 XHG=1529 XHG=1640 XHG=1721 XHG=1829 XHG=1919 XHG=2077 XHG=2218 XHG=2287 XHG=2492 YBD=1788 YBD=1796 YBD=1794 YBD=1787 YHG=1758>removed YHG=1757>from YHG=1759>the YHG=1759>bath YHG=1758>and YHG=1758>rapidly YHG=1758>cooled YHG=1758>by YHG=1758>immersion YHG=1758>in XBD=237 XHG=803 XBD=293 XBD=506 XBD=613 XBD=790 XBD=984 XBD=1105 XBD=1150 XBD=1285 XHG=84 XHG=251 XHG=303 XHG=523 XHG=629 XHG=1024 XHG=1121 XHG=1165 YBD=1823 YBD=1829 YBD=1823 YBD=1831 YBD=1822 YHG=1795>solution YHG=1794>of YHG=1794>hyaluronic YHG=1794>acid, YHG=1795>however YHG=1793>prepared. YHG=1794>This YHG=1794>is YHG=1794>mixed " XBD=2521 XBD=1492 XBD=1619 XBD=1676 XBD=1756 XBD=1994 XBD=2068 XBD=2108 XBD=2212 XBD=2342 XBD=2454 XHG=1343 XHG=1510 XHG=1639 XHG=1695 XHG=1775 XHG=2013 XHG=2089 XHG=2129 XHG=2260 XHG=2361 XHG=2471 YBD=1827 YBD=1834 YBD=1826 YHG=1799>ice-cold YHG=1801>water YHG=1799>in YHG=1799>the YHG=1798>refrigerator YHG=1797>for YHG=1800>5 YHG=1798>min. YHG=1798>This YHG=1801>stops YHG=1798>the " XBD=1282 XBD=157 XBD=252 XBD=442 XBD=608 XBD=781 XBD=892 XBD=1041 XBD=1120 XBD=1196 XHG=85 XHG=192 XHG=288 XHG=477 XHG=668 XHG=926 XHG=1068 XHG=1154 XHG=1221 YBD=1863 YBD=1863 YBD=1862 YBD=1861 YBD=1869 YBD=1869 YBD=1862 YHG=1835>and YHG=1835>the YHG=1834>mixture YHG=1834>cooled. YHG=1833>When YHG=1834>cool YHG=1834>(<10° YHG=1834>C.) YHG=1834>75 YHG=1833>ml. XBD=1500XHG=816 XBD=1640 XBD=1730 XBD=1922 XBD=2002 XBD=2160 XBD=2220 XBD=2297 XBD=2386 XBD=2521 XHG=1343 XHG=1522 XHG=1660 XHG=1749 XHG=1942 XHG=2023 XHG=2180 XHG=2236 XHG=2317 XHG=2404 YBD=1874 YBD=1875 YBD=1865 YBD=1866 YHG=1838>enzymic YHG=1838>action YHG=1838>and YHG=1838>improves YHG=1838>the YHG=1837>quality YHG=1836>of YHG=1837>the YHG=1837>clot YHG=1837>formed XBD=106 XHG=748 XBD=164 XBD=389 XBD=434 XBD=568 XBD=655 XBD=732 XBD=901 XBD=1060 XBD=1125 XBD=1283 XHG=87 XHG=120 XHG=181 XHG=404 XHG=451 XHG=584 XHG=672 XHG=918 XHG=1092 XHG=1136 YBD=1902 YBD=1903 YBD=1911 YBD=1902 YBD=1901 YBD=1902 YBD=1901 YBD=1902 YHG=1875>2 YHG=1874>N. YHG=1873>CHCOOH YHG=1874>is YHG=1874>added YHG=1873>and YHG=1873>the YHG=1873>mixture YHG=1873>shaken: YHG=1873>A YHG=1873>volumi" XBD=2521 XBD=1390 XBD=1631 XBD=1817 XBD=1884 XBD=2016 XBD=2119 XBD=2217 XBD=2327 XHG=1343 XHG=1417 XHG=1659 XHG=1844 XHG=1908 XHG=2041 XHG=2146 XHG=2244 XHG=2353 YBD=1914 YBD=1906 YBD=1905 YHG=1886>on YHG=1878>subsequent YHG=1877>addition YHG=1877>of YHG=1877>acetic YHG=1877>acid YHG=1877>and YHG=1876>thus YHG=1876>facilitates " XBD=1283 XBD=173 XBD=266 XBD=466 XBD=627 XBD=672 XBD=786 XBD=1030 XBD=1150 XHG=84 XHG=196 XHG=290 XHG=489 XHG=651 XHG=808 XHG=1054 XHG=1209 YBD=1942 YBD=1942 YBD=1951 YBD=1950 YBD=1941 YBD=1941 YBD=1943 YBD=1941 YHG=1922>nous YHG=1914>clot YHG=1915>separates YHG=1913>leaving YHG=1922>a YHG=1913>clear YHG=1912>non-viscous YHG=1912>fluid. YHG=1912>The XBD=1615XHG=695 XBD=1675 XBD=1751 XBD=1940 XBD=2000 XBD=2174 XBD=2256 XBD=2304 XBD=2362 XBD=2453 XBD=2522 XHG=1343 XHG=1635 XHG=1690 XHG=1772 XHG=1960 XHG=2016 XHG=2219 XHG=2276 XHG=2324 XHG=2379 XHG=2469 YBD=1946 YBD=1953 YBD=1945 YBD=1954 YHG=1917>determination YHG=1916>of YHG=1917>the YHG=1917>endpoint YHG=1917>of YHG=1916>activity. YHG=1916>It YHG=1916>is YHG=1926>as YHG=1916>well YHG=1922>not " XBD=2520 XBD=1381 XBD=1472 XBD=1550 XBD=1675 XBD=1772 XBD=1826 XBD=1938 XBD=2044 XBD=2131 XBD=2237 XBD=2294 XBD=2475 XHG=1342 XHG=1400 XHG=1488 XHG=1567 XHG=1694 XHG=1788 XHG=1843 XHG=1956 XHG=2060 XHG=2147 XHG=2254 XHG=2307 XHG=2491 YBD=1986 YBD=1985 YBD=1984 YHG=1960>to YHG=1957>add YHG=1957>the YHG=1957>acetic YHG=1957>acid YHG=1959>to YHG=1966>more YHG=1957>than YHG=1959>two YHG=1965>rows YHG=1956>of YHG=1956>titrations YHG=1949>at XBD=156 XHG=878 XBD=204 XBD=388 XBD=567 XBD=631 XBD=670 XBD=779 XBD=860 XBD=949 XBD=1136 XBD=1193 XBD=1283 XHG=84 XHG=173 XHG=221 XHG=406 XHG=584 XHG=649 XHG=687 XHG=796 XHG=967YBD=1982 XHG=1154 XHG=1210 YBD=1982 YBD=1990 YBD=1981 YBD=1991 YBD=1981 YHG=1953>clot YHG=1953>is YHG=1953>gathered YHG=1953>together YHG=1962>on YHG=1963>a YHG=1953>glass YHG=1953>rod YHG=1952>and YHG=1953>squeezed YHG=1962>as YHG=1951>free

XBD=124 XHG=847 XBD=229 XBD=287 XBD=469 XBD=553 XBD=603 XBD=757 XBD=825 XBD=896 XBD=1012 XBD=1104 XBD=1284 XHG=84 XHG=142 XHG=248 XHG=308 XHG=516 XHG=574 XHG=624 XHG=777 XHG=916 XHG=1033 XHG=1125 YBD=2021 YBD=2022 YBD=2030 YBD=2021 YBD=2029 YBD=2021 YBD=2022 YBD=2030 YHG=1992>of YHG=1992>fluid YHG=2002>as YHG=1993>possible. YHG=1993>It YHG=1993>is YHG=1993>broken YHG=2002>up YHG=1992>by YHG=1992>hand YHG=1992>and YHG=1991>dropped " XBD=2521 XBD=1366 XBD=1478 XBD=1544 XBD=1674 XBD=1888 XBD=2056 XBD=2123 XBD=2206 XBD=2310 XBD=2448 XHG=1343 XHG=1391 XHG=1504 XHG=1569 XHG=1698 XHG=1914 XHG=2082 XHG=2145 XHG=2233 XHG=2335 XHG=2470 YBD=2026 YBD=2034 YBD=2025 YBD=2024 YHG=2006>a YHG=1997>time YHG=1999>to YHG=1997>avoid YHG=1996>prolonged YHG=1999>contact YHG=1996>of YHG=1996>acid YHG=1996>before YHG=1996>the XBD=159 XBD=325 XBD=416 XBD=520 XBD=626 XBD=689 XBD=808 XBD=880 XBD=1037 XBD=1095 XBD=1176 XBD=1283 XHG=83 XHG=180 XHG=345 XHG=435 XHG=539 XHG=645 XHG=710 XHG=826 XHG=900 XHG=1056 XHG=1114 XHG=1195 YBD=2061 YBD=2061 YBD=2068 YBD=2060 YBD=2069 YBD=2060 YHG=2033>into YHG=2035>acetone YHG=2033>and YHG=2041>may YHG=2032>then YHG=2033>be YHG=2032>dried YHG=2031>for YHG=2035>storage YHG=2041>or YHG=2032>the YHG=2034>next " XBD=2463 |>" XBD=1431 XBD=1518 XBD=1639 XBD=1786 XBD=1873 XBD=2060 XBD=2128 XBD=2212 XBD=2318 XBD=2373 XBD=2521 XHG=1342 XHG=1459 XHG=1545 XHG=1685 XHG=1812 XHG=1901 XHG=2087 XHG=2151 XHG=2239 XHG=2344 XHG=2398 XHG=2485 YBD=2066 YBD=2064 YBD=2065 YBD=2071 YHG=2039>tests YHG=2045>are YHG=2037>read. YHG=2036>After YHG=2037>the YHG=2036>addition YHG=2036>of YHG=2036>the YHG=2036>acid YHG=2036>it YHG=2035>will YHG=2035>be XBD=182 XHG=685 XBD=305 XBD=387 XBD=648 XBD=834 XBD=1009 XBD=1160 XBD=1282 XHG=84 XHG=221 XHG=343 XHG=427 XHG=873 XHG=1049 XHG=1208 YBD=2109 YBD=2109 YBD=2100 YBD=2100 YBD=2108 YBD=2099 YHG=2075>stage YHG=2081>may YHG=2072>be YHG=2072>undertaken YHG=2072>without YHG=2071>further YHG=2071>delay. YHG=2071>The " XBD=2524 XBD=1484 XBD=1585 XBD=1629 XBD=1746 XBD=1807 XBD=2029 XBD=2158 XBD=2219 XBD=2303 XBD=2474 XHG=1342 XHG=1505 XHG=1608 XHG=1650 XHG=1767 XHG=1824 XHG=2051 XHG=2180 XHG=2240 XHG=2322 XHG=2492 YBD=2105 YBD=2113 YBD=2104 YBD=2112 YBD=2102 YHG=2076>noticed YHG=2076>that YHG=2086>a YHG=2076>layer YHG=2075>of YHG=2076>precipitate YHG=2075>forms YHG=2078>at YHG=2076>the YHG=2076>junction YHG=2073>of " XBD=2520 XBD=1404 XBD=1509 XBD=1604 XBD=1690 XBD=1785 XBD=1960 XBD=2023 XBD=2094 XBD=2245 XBD=2353 XBD=2405 XBD=2465 XHG=1341 XHG=1429 XHG=1534 XHG=1629 XHG=1715 XHG=1809 XHG=1986 XHG=2047 XHG=2118 XHG=2283 XHG=2377 XHG=2429 XHG=2482 YBD=2144 YBD=2143 YBD=2142 YHG=2115>the YHG=2115>acid YHG=2116>and YHG=2116>the YHG=2118>test YHG=2116>mixture YHG=2116>in YHG=2116>all YHG=2116>tubes YHG=2115>this YHG=2115>is YHG=2114>of YHG=2123>no : XBD=289 XHG=728 XBD=332 XBD=480 XBD=536 XBD=659 XBD=711 XBD=888 XBD=1039 XBD=1093 XBD=1132 XBD=1283 XHG=82 XHG=303 XHG=345 XHG=497 XHG=553 XHG=674 XHG=903 XHG=1056 XHG=1110 XHG=1148 YBD=2149 YBD=2147 YBD=2140 YBD=2148 YBD=2148 YBD=2140 YBD=2139 YHG=2111>precipitate YHG=2112>is YHG=2112>broken YHG=2111>finely YHG=2121>as YHG=2111>possible, YHG=2112>ground YHG=2111>in YHG=2121>a YHG=2113>mortar XBD=121 XHG=884 XBD=163 XBD=321 XBD=370 XBD=599 XBD=814 XBD=866 XBD=993 XBD=1068 XBD=1226 XBD=1286 XHG=83 XHG=141 XHG=179 XHG=338 XHG=384 XHG=617 XHG=830 XHG=1014 XHG=1086 XHG=1245 YBD=2180 YBD=2188 YBD=2179 YBD=2180 YBD=2188 YBD=2180 YBD=2179 YHG=2155>to YHG=2161>a YHG=2151>powder YHG=2150>if YHG=2151>dried-and YHG=2151>suspended YHG=2150>in YHG=2154>water YHG=2152>1/5 YHG=2150>volume YHG=2150>of " XBD=2521 XBD=1536 XBD=1789 XBD=1908 XBD=2033 XBD=2115 XBD=2217 XBD=2322 XBD=2390 XBD=2449 XHG=1343 XHG=1561 XHG=1835 XHG=1931 XHG=2057 XHG=2139 XHG=2241 XHG=2343 XHG=2413 XHG=2470 YBD=2185 YBD=2184 YBD=2192 YBD=2183 YBD=2182 YHG=2156>diagnostic YHG=2155>significance. YHG=2155>The YHG=2156>tubes YHG=2164>are YHG=2164>now YHG=2155>held YHG=2163>up YHG=2157>to YHG=2154>the XBD=229 XBD=396 XBD=476 XBD=563 XBD=689 XBD=750 XBD=872 XBD=1044 XBD=1189 XBD=1281 XHG=84 XHG=248 XHG=443 XHG=495 XHG=586 XHG=712 XHG=767 XHG=892 XHG=1066 XHG=1212 YBD=2227 YBD=2220 YBD=2227 YBD=2219 YBD=2219 YBD=2228 YBD=2226 YHG=2191>original YHG=2193>extract. YHG=2191>A YHG=2190>few YHG=2191>drops YHG=2190>of YHG=2190>KOH YHG=2191>solution YHG=2191>(about YHG=2190>5N) |>" " XBD=1431 XBD=1665 XBD=1828 XBD=1946 XBD=2102 XBD=2194 XBD=2314 XBD=2433 XBD=2520 XBD=1525 XHG=1340 XHG=1542 XHG=1685 XHG=1873 XHG=1967 XHG=2123 XHG=2214 XHG=2334 XHG=2453 XHG=1447 YBD=2232 YBD=2231 YBD=2225 YBD=2224 YBD=2223 YBD=2225 YHG=2195>light YHG=2195>gently YHG=2195>shaken. YHG=2195>The YHG=2195>control YHG=2195>and YHG=2195>those YHG=2195>tubes YHG=2203>conYHG=2196>and " XBD=142 XBD=286 XBD=383 XBD=472 XBD=666 XBD=928 XBD=1139 XHG=84 XHG=169 XHG=312 XHG=409 XHG=498 XHG=956 XHG=1185 YBD=2259 YBD=2259 YBD=2261 YBD=2258 YBD=2260 YBD=2258 YHG=2240>are shaken YHG=2230>added YHG=2231>and YHG=2231>the YHG=2231>mixture YHG=2230>until YHG=2230>dissolved. YHG=2230>More " XBD=1281 XBD=1479XHG=837 XBD=1716 XBD=1890 XBD=2016 XBD=2107 XBD=2381 XBD=2446 XBD=2520 XHG=1341 XHG=1508 XHG=1746 XHG=1922 XHG=2046 XHG=2139 XHG=2409 XHG=2469 YBD=2271 YBD=2264 YBD=2272 YBD=2263 YBD=2262 YHG=2235>taining YHG=2234>insufficient YHG=2245>enzyme YHG=2235>show YHG=2235>the YHG=2235>development YHG=2233>of YHG=2234>the XBD=188 XHG=882 XBD=291 XBD=354 XBD=489 XBD=539 XBD=744 XBD=864 XBD=966 XBD=1137 XBD=1282 XHG=83 XHG=207 XHG=310 XHG=373 XHG=507 XHG=552 XHG=792 XHG=985 XHG=1155 YBD=2299 YBD=2299 YBD=2306 YBD=2298 YBD=2299 YBD=2298 YHG=2270>KOH YHG=2279>may YHG=2270>be YHG=2270>added YHG=2269>if YHG=2278>necessary. YHG=2269>The YHG=2270>final YHG=2270>solution YHG=2269>should " XBD=2520 XBD=1599 XBD=1752 XBD=1839 XBD=1969 XBD=2163 XBD=2226 XBD=2414 XHG=1341 XHG=1618 XHG=1769 XHG=1856 XHG=1987 XHG=2180 XHG=2243 XHG=2457 YBD=2304 YBD=2311 YBD=2303 YHG=2275>characteristic YHG=2274>stringy YHG=2274>clot YHG=2275>which YHG=2277>contracts YHG=2284>on YHG=2274>standing. YHG=2274>One XBD=126 XHG=761 XBD=215 XBD=329 XBD=427 XBD=541 XBD=715 XBD=834 XBD=1012 XBD=1066 XBD=1179 XBD=1279 XHG=83 XHG=153 XHG=243 XHG=356 XHG=453 XHG=566 XHG=860 XHG=1037 XHG=1092 XHG=1204 YBD=2338 YBD=2346 YBD=2338 YBD=2337 YBD=2338 YBD=2338 YBD=2337 YBD=2346 YHG=2310>be YHG=2309>pH YHG=2310>9-10 YHG=2310>and YHG=2319>very YHG=2309>viscous. YHG=2309>The YHG=2310>solution YHG=2310>is YHG=2309>then YHG=2318>pre" XBD=2522 XBD=1380 XBD=1472 XBD=1595 XBD=1700 XBD=1781 XBD=1970 XBD=2074 XBD=2189 XBD=2233 XBD=2318 XBD=2471 XHG=1341 XHG=1400 XHG=1493 XHG=1615 XHG=1720 XHG=1801 XHG=1990 XHG=2094 XHG=2211 XHG=2252 XHG=2338 XHG=2489 YBD=2351 YBD=2344 YBD=2343 YBD=2342 YHG=2324>or YHG=2317>two YHG=2315>tubes YHG=2324>near YHG=2313>the YHG=2315>endpoint YHG=2324>may YHG=2314>show YHG=2324>a YHG=2313>few YHG=2314>threads YHG=2312>of |>" " XBD=251 XBD=352 XBD=585 XBD=641 XBD=869 XBD=983 XBD=1180 XBD=1291 XBD=412 XHG=82 XHG=266 XHG=426 XHG=601 XHG=651 XHG=884 XHG=999 XHG=1194 XHG=372 YBD=2385 YBD=2377 YBD=2376 YBD=2384 YBD=2385 YBD=2377 YBD=2385 YBD=2383 YHG=2349>cipitated YHG=2349>with YHG=2349>volumes YHG=2348>of YHG=2348>methylated YHG=2349>spirit YHG=2349>saturated YHG=2350>I YHG=2348>with. XBD=1559 XBD=1717 XBD=1839 XBD=1896 XBD=2027 XBD=2106 XBD=2271 XBD=2352 XBD=2519 XHG=1339 XHG=1605 XHG=1737 XHG=1859 XHG=1914 XHG=2046 XHG=2127 XHG=2290 XHG=2370 YBD=2391 YBD=2382 YHG=2354>precipitate. YHG=2354>Those YHG=2354>tubes YHG=2355>in YHG=2355>which YHG=2355>the YHG=2363>enzyme YHG=2354>has YHG=2352>attacked XBD=277 XHG=887 XBD=432 XBD=520 XBD=567 XBD=612 XBD=716 XBD=871 XBD=973 XBD=1012 XBD=1118 XBD=1280 XHG=82 XHG=294 XHG=449 XHG=537 XHG=584 XHG=630 XHG=732 XHG=990 XHG=1029 XHG=1137 YBD=2425 YBD=2417 YBD=2415 YBD=2416 YBD=2416 YBD=2416 YHG=2388>potassium YHG=2390>acetate YHG=2388>and YHG=2388>it YHG=2388>is YHG=2388>then YHG=2387>washed YHG=2388>with YHG=2397>a YHG=2388>little YHG=2390>acetone " XBD=2518 XBD=1402 XBD=1597 XBD=1711 XBD=1776 XBD=1866 XBD=1923 XBD=2146 XBD=2236 XBD=2352 XBD=2405 XHG=1339 XHG=1421 XHG=1616 XHG=1728 XHG=1794 XHG=1883 XHG=1939 XHG=2165 XHG=2253 XHG=2370 XHG=2420 YBD=2421 YBD=2431 YBD=2422 YHG=2393>the YHG=2393>substrate YHG=2393>show YHG=2402>no YHG=2393>clot YHG=2403>or YHG=2394>precipitate YHG=2393>and YHG=2393>those YHG=2394>in YHG=2393>which " XBD=1072 XBD=138 XBD=349 XBD=476 XBD=564 XBD=677 XBD=729 XBD=855 XBD=954 XHG=86 XHG=152 XHG=364 XHG=493 XHG=580 XHG=692 XHG=744 XHG=969 YBD=2463 YBD=2463 YBD=2455 YBD=2465 YHG=2428>(or YHG=2426>preferably YHG=2427>ether) YHG=2427>and YHG=2427>dried YHG=2427>in YHG=2436>vacuo YHG=2436>over YHG=2428>P2Os’ XBD=1402XHG=871 XBD=1586 XBD=1653 XBD=1831 XBD=1907 XBD=2061 XBD=2194 XBD=2379 XBD=2444 XBD=2518 XHG=1340 XHG=1441 XHG=1624 XHG=1690 XHG=1869 XHG=1943 XHG=2098 XHG=2232 XHG=2408 XHG=2468 YBD=2469 YBD=2462 YBD=2470 YBD=2461 YBD=2460 YHG=2438>enzyme YHG=2433>is YHG=2435>present YHG=2433>in YHG=2443>excess YHG=2433>show YHG=2433>clearing YHG=2432>of YHG=2433>the XBD=184 XHG=829 XBD=308 XBD=393 XBD=530 XBD=698 XBD=805 XBD=937 XBD=1137 XBD=1200 XBD=1280 XHG=128 XHG=208 XHG=332 XHG=416 XHG=552 XHG=722 XHG=961YBD=2495 XHG=1160 XHG=1219 YBD=2503 YBD=2494 YBD=2502 YBD=2495 YBD=2495 YHG=2467>By YHG=2467>using YHG=2467>the YHG=2467>above YHG=2466>method YHG=2466>only YHG=2467>about YHG=2467>one-third YHG=2467>of YHG=2467>the " XBD=2324 XBD=1503 XBD=1623 XBD=1766 XBD=1844 XBD=2046 XBD=2103 XBD=2255 XHG=1340 XHG=1551 XHG=1642 XHG=1786 XHG=1862 XHG=2065 XHG=2122 XHG=2271 YBD=2501 YBD=2510 YBD=2502 YHG=2473>solution. YHG=2472>The YHG=2473>results YHG=2482>are YHG=2474>expressed YHG=2482>as YHG=2473>follows YHG=2484>:― " XBD=225 XBD=279 XBD=445 XBD=489 XBD=675 XBD=757 XBD=802 XBD=844 XBD=929 XBD=1096 XBD=1199 XHG=81 XHG=239 XHG=290 XHG=460 XHG=504 XHG=692 XHG=772 XHG=816 XHG=951 XHG=1117 XHG=1219 YBD=2534 YBD=2536 YBD=2543 YBD=2534 YBD=2534 YBD=2534 YHG=2506>’volume YHG=2506>of YHG=2506>solvents YHG=2508>is YHG=2506>required, YHG=2506>but YHG=2506>it YHG=2506>is YHG=2509>not YHG=2506>claimed YHG=2506>that YHG=2506>the " XBD=1280 XBD=1471XHG=865 XBD=1552 XBD=1602 XBD=1744 XBD=1792 XBD=2089 XBD=2280 XBD=2424 XHG=1421 XHG=1487 XHG=1566 XHG=1614 XHG=1758 XHG=1805 XHG=2137 XHG=2292 YBD=2556 YBD=2560 YBD=2561 YHG=2531>No YHG=2532>clot YHG=2538>or YHG=2530>threads YHG=2531>of YHG=2531>precipitate.. YHG=2532>Enzyme YHG=2530>present. " XBD=188 XBD=423 XBD=482 XBD=552 XBD=669 XBD=739 XBD=807 XBD=897 XBD=1072 XBD=1142 XHG=84 XHG=197 XHG=453 XHG=514 XHG=582 XHG=701 XHG=769 XHG=927 XHG=1102 XHG=1171 YBD=2572 YBD=2581 YBD=2572 YBD=2582 YBD=2573 YBD=2573 YBD=2573 YHG=2544>final. YHG=2545>preparation YHG=2545>is YHG=2554>pure YHG=2554>as YHG=2545>in YHG=2545>the YHG=2545>method YHG=2545>of YHG=2546>direct " XBD=1280 XBD=1562XHG=837 XBD=1610 XBD=2085 XBD=2170 XHG=1421 XHG=1576 XHG=1622 XHG=2140 YBD=2593 YBD=2596 YBD=2594 YHG=2568>Threads YHG=2568>of YHG=2567>precipitate...... YHG=2568>t " XBD=2337 XBD=329 XHG=818 XBD=494 XBD=573 XBD=749 XBD=806 XBD=933 XBD=1112 XHG=83 XHG=344 XHG=512 XHG=591 XHG=765 XHG=948 YBD=2620 YBD=2612 YBD=2613 YBD=2621 YHG=2583>precipitation YHG=2584>without YHG=2585>the YHG=2584>addition YHG=2584>of YHG=2592>serum YHG=2584>proteins. " XBD=2086 XBD=2182 XHG=1420 XHG=2133 XHG=2197 YBD=2631 YBD=2634 YHG=2604>Clot.......... YHG=2605>No YHG=2611>enzyme. " XBD=1074 XBD=542 XBD=820 XBD=953 XHG=295 XHG=564 XHG=846 XHG=978 YBD=2678 YBD=2679 YBD=2685 YBD=2678 YHG=2655>MUCIN-CLOT YHG=2655>PREVENTION YHG=2649>(MCP) YHG=2655>TEST XBD=1458 XHG=2485 XBD=1612 XBD=1779 XBD=1949 XBD=2011 XBD=2099 XBD=2156 XBD=2313 XBD=2357 XBD=2471 XBD=2516 XHG=1384 YBD=2686 XHG=1474 XHG=1629 XHG=1794 XHG=1965 XHG=2029 XHG=2116 XHG=2171 XHG=2328 XHG=2372 YBD=2693 YHG=2665>as YBD=2684 YBD=2692 YBD=2685 YHG=2657>The YHG=2657>highest YHG=2656>dilution YHG=2656>showing YHG=2666>no YHG=2658>clot YHG=2667>or YHG=2657>threads YHG=2658>is YHG=2658>taken " XBD=2515 XBD=1401 XBD=1572 XBD=1764 XBD=1947 XBD=2018 XBD=2108 XBD=2294 XBD=2410 XHG=1339 XHG=1433 XHG=1604 XHG=1796 XHG=1979 XHG=2047 XHG=2140 XHG=2349 XHG=2439 YBD=2733 YBD=2724 YBD=2734 YBD=2725 YBD=2726 YHG=2696>the YHG=2696>highest YHG=2696>effective YHG=2695>dilution YHG=2696>of YHG=2697>the YHG=2696>enzyme. YHG=2696>We YHG=2696>have XBD=228 XHG=874 XBD=329 XBD=522 XBD=654 XBD=743 XBD=850 XBD=964 XBD=1279 XHG=136 XHG=250 XHG=356 XHG=548 XHG=676 XHG=769 XHG=989YBD=2727 YBD=2735 YBD=2736 YBD=2728 YBD=2735 YHG=2696>This YHG=2696>test YHG=2697>depends YHG=2707>upon YHG=2697>the YHG=2697>fact YHG=2697>that YHG=2697>hyaluronidase " XBD=2518 XBD=1526 XBD=1617 XBD=1769 XBD=1834 XBD=1969 XBD=2038 XBD=2123 XBD=2296 XBD=2463 XHG=1340 XHG=1555 XHG=1645 XHG=1798 XHG=1863 XHG=1998 XHG=2062 XHG=2152 XHG=2324 XHG=2486 YBD=2772 YBD=2763 YBD=2773 YBD=2766 YBD=2764 YHG=2736>expressed YHG=2744>our YHG=2735>results YHG=2735>in YHG=2738>terms YHG=2736>of YHG=2736>the YHG=2736>original YHG=2736>dilution YHG=2734>of XBD=185 XHG=755 XBD=254 XBD=422 XBD=492 XBD=578 XBD=732 XBD=926 XBD=1017 XBD=1222 XBD=1281 XHG=85 XHG=203 XHG=272 XHG=449 XHG=517 XHG=606 XHG=952 XHG=1047 XHG=1242 YBD=2766 YBD=2766 YBD=2767 YBD=2774 YBD=2767 YBD=2775 YBD=2767 YHG=2736>from YHG=2736>all YHG=2745>sources YHG=2746>so YHG=2738>far YHG=2736>tested YHG=2737>destroys YHG=2737>the YHG=2737>capacity YHG=2738>of |>" " XBD=1486 XBD=1554 XBD=1728 XBD=1832 XBD=1952 XBD=2204 XBD=2294 XBD=2513 XBD=1640 XHG=1340 XHG=1517 XHG=1669 XHG=1762 XHG=1861 XHG=1983 XHG=2233 XHG=2323 XHG=1585 YBD=2811 YBD=2802 YBD=2803 YBD=2812 YBD=2804 YBD=2813 YBD=2803 YHG=2784>enzyme YHG=2775>in YHG=2775>ml. YHG=2775>and YHG=2774>have YHG=2775>disregarded YHG=2775>the YHG=2776>subsequent YHG=2776>0-5 XBD=110 XBD=527 XBD=643 XBD=844 XBD=918 XBD=1050 XBD=1104 XBD=1280 XHG=86 XHG=141 XHG=556 XHG=673 XHG=874 XHG=951 XHG=1080 XHG=1136 YBD=2804 YBD=2813 YBD=2814 YBD=2806 YBD=2806 YBD=2805 YBD=2814 YHG=2784>a YHG=2775>protein-hyaluronic YHG=2776>acid YHG=2777>complex YHG=2776>to YHG=2776>form YHG=2785>a YHG=2776>typical " XBD=255 XBD=403 XBD=489 XBD=587 XBD=797 XBD=868 XBD=1021 XBD=1150 XHG=90 XHG=288 XHG=438 XHG=520 XHG=620 XHG=900 XHG=1053 XHG=1201 YBD=2843 YBD=2843 YBD=2844 YBD=2845 YBD=2845 YBD=2845 YHG=2814>&dquo; YHG=2813>clot&dquo; YHG=2823>on YHG=2815>the YHG=2815>addition YHG=2815>of YHG=2815>acetic YHG=2814>acid. YHG=2815>The mucin " XBD=1279 XBD=1492XHG=830 XBD=1592 XBD=1786 XBD=1966 XBD=2049 XBD=2097 XBD=2333 XBD=2436 XBD=2481 XBD=2515 XHG=1340 XHG=1506 XHG=1611 XHG=1805 XHG=2013 XHG=2068 XHG=2115 XHG=2350 XHG=2450 XHG=2498 YBD=2843 YBD=2852 YBD=2850 YBD=2844 YBD=2842 YHG=2814>dilution YHG=2813>with YHG=2814>substrate YHG=2814>mixture. YHG=2815>It YHG=2815>is YHG=2814>emphasised YHG=2814>that, YHG=2825>as YHG=2824>a

"|>"XBD=2514 XBD=1797 XBD=1849 XBD=1900 XBD=2051 XBD=2153 XBD=2212 XBD=2335 XBD=2428 XBD=1608 XHG=1629 XHG=1821 XHG=1871 XHG=1921 XHG=2071 XHG=2174 XHG=2232 XHG=2355 XHG=2447 XHG=1338 YBD=2886 YBD=2881 YBD=2880 YBD=2888 YBD=2882 YBD=2883 YBD=2889 YHG=2862>measure, YHG=2854>it YHG=2852>is YHG=2853>always YHG=2853>wise YHG=2857>to YHG=2854>make YHG=2863>sure YHG=2855>that YHG=2847>precautionary XBD=237 XBD=420 XBD=480 XBD=564 XBD=740 XBD=903 XBD=1042 XBD=1139 XBD=1280 XHG=85 XHG=255 XHG=440 XHG=497 XHG=585 XHG=761 XHG=923 XHG=1060 XHG=1156 YBD=2890 YBD=2882 YBD=2883 YBD=2891 YBD=2892 YBD=2883 YBD=2884 YHG=2853>highest YHG=2852>dilution YHG=2853>of YHG=2853>the YHG=2863>enzyme YHG=2854>sample YHG=2854>under YHG=2854>test YHG=2853>which " XBD=2516 XBD=1401 XBD=1737 XBD=1919 XBD=2017 XBD=2116 XBD=2192 XBD=2282 XBD=2460 XHG=1339 XHG=1431 XHG=1766 XHG=1947 XHG=2045 XHG=2146 XHG=2221 XHG=2310 XHG=2484 YBD=2920 YBD=2921 YBD=2922 YHG=2892>the YHG=2891>serum-substrate YHG=2892>mixture YHG=2892>will YHG=2893>clot YHG=2902>on YHG=2893>the YHG=2893>addition YHG=2890>of XBD=161 XHG=772 XBD=323 XBD=426 XBD=750 XBD=871 XBD=1115 XBD=1233 XBD=1280 XHG=83 XHG=183 XHG=345 XHG=451 XHG=893 XHG=1137 XHG=1256 YBD=2923 YBD=2922 YBD=2921 YBD=2923 YBD=2923 YBD=2923 YHG=2892>will YHG=2892>inhibit YHG=2892>this YHG=2892>clot-formation YHG=2893>after YHG=2892>incubation YHG=2893>with YHG=2902>a XBD=1450XHG=830 XBD=1556 XBD=1699 XBD=1854 XBD=1934 XBD=2016 XBD=2141 XBD=2270 XBD=2376 XBD=2474 XBD=2513 XHG=1340 XHG=1478 XHG=1582 XHG=1725 XHG=1880 XHG=1955 XHG=2042 XHG=2187 XHG=2295 XHG=2401 XHG=2495 YBD=2961 YBD=2967 YBD=2975 YBD=2959 YBD=2969 YBD=2962 YBD=2960 YHG=2931>acetic YHG=2931>acid YHG=2930>before YHG=2931>setting YHG=2940>up YHG=2931>the YHG=2934>tests. YHG=2932>This YHG=2939>may YHG=2942>save YHG=2941>a "" XBD=1279 XBD=269 XBD=485 XBD=675 XBD=758 XBD=806 XBD=931 XBD=1045 XHG=84 XHG=293 XHG=510 XHG=699 XHG=781 XHG=952 XHG=1126 YBD=2962 YBD=2961 YBD=2962 YBD=2962 YBD=2970 YHG=2931>standard YHG=2931>substrate YHG=2931>mixture YHG=2933>for YHG=2941>a YHG=2932>fixed YHG=2932>time YHG=2941>propori XBD=1576 XBD=1713 XBD=1783 XBD=1894 XBD=1961 XBD=2052 XBD=2186 XBD=2257 XBD=2352 XHG=1339 XHG=1605 XHG=1744 XHG=1809 XHG=1925 XHG=1990 XHG=2082 XHG=2217 XHG=2282 XHG=2379 YBD=2998 YBD=2999 YBD=3000 YHG=2970>considerable YHG=2973>waste YHG=2970>time YHG=2970>in YHG=2970>the YHG=2973>event YHG=2970>of YHG=2970>this YHG=2972>mixture " XBD=2512 XBD=205 XHG=689 XBD=263 XBD=346 XBD=530 XBD=589 XBD=669 XBD=847 XBD=1018 XHG=81 XHG=220 XHG=280 XHG=364 XHG=549 XHG=603 XHG=862YBD=3001 YBD=3000 YBD=2999 YBD=3008 YBD=3009 YHG=2971>tional YHG=2971>to YHG=2969>potency YHG=2970>of YHG=2970>the YHG=2971>original YHG=2971>sample. "" XBD=2512 XBD=1470 XBD=1584 XBD=1869 XBD=1983 XBD=2079 XBD=2216 XBD=2382 XHG=1338YBD=3066 XHG=1496 XHG=1608 XHG=1892 XHG=2008 XHG=2103 XHG=2264 XHG=2405 YBD=3037YHG=3045>MIXTURE YBD=3045 YBD=3046 YBD=3038 YBD=3040 YHG=3009>having YHG=3009>been YHG=3005>contaminated YHG=3008>from YHG=3018>any YHG=3019>cause. YHG=3010>Serum YHG=3009>should XBD=760 XHG=783 XBD=952 XHG=411 YBD=3068 YHG=3045>SERUM-SUBSTRATE

" XBD=2511 XBD=1446 XHG=2445 XBD=1509 XBD=1615 XBD=1724 XBD=1791 XBD=1962 XBD=2112 XBD=2196 XBD=2286 XBD=2429 XHG=1338 YBD=3078 XHG=1465 XHG=1530 XHG=1634 XHG=1744 XHG=1811 XHG=1983 XHG=2131 XHG=2216 XHG=2305 YBD=3076 YHG=3050>that YBD=3077 YBD=3085 YBD=3079 YHG=3057>never YHG=3048>be YHG=3048>used YHG=3048>from YHG=3057>an YHG=3048>infected YHG=3043>nor YHG=3058>any YHG=3048>animal " XBD=1403 XBD=1511 XBD=1736 XBD=1793 XBD=1882 XBD=1985 XBD=2146 XBD=2509 XHG=1338 XHG=1422 XHG=1530 XHG=1755 XHG=1812 XHG=1901 XHG=2002 XHG=2164 YBD=3115 YBD=3123 YBD=3116 YBD=3124 YHG=3088>has YHG=3088>been YHG=3087>immunised YHG=3096>as YHG=3088>this YHG=3096>may YHG=3088>contain YHG=3088>anti-hyaluronidase. |>" XBD=601 XBD=806 XBD=1051 XBD=1130 XBD=1279 XBD=545 XHG=561 XHG=611 XHG=820 XHG=1062 XHG=1145 XHG=124 YBD=3114 YBD=3123 YBD=3123 YBD=3117 YHG=3086>of YHG=3086>potassium YHG=3087>hyaluronate YHG=3086>Substrate.-A solution YHG=3089>(see YHG=3096>accomXBD=1416XHG=842 XBD=1492 XBD=1652 XBD=1716 XBD=1886 XBD=2011 XBD=2064 XBD=2126 XBD=2510 XHG=1385 XHG=1443 XHG=1506 XHG=1666 XHG=1731 XHG=1899 XHG=2026 XHG=2074 XHG=2139 YBD=3161 YBD=3163 YHG=3129>5. YHG=3127>To YHG=3126>indicate YHG=3126>the YHG=3127>bacterial YHG=3135>source YHG=3126>of YHG=3127>the YHG=3126>hyalurouidase.-This " XBD=1281 XBD=236 XBD=465 XBD=521 XBD=771 XBD=826 XBD=994 XBD=1117 XBD=1160 XHG=80 XHG=252 XHG=481 XHG=532 XHG=789 XHG=1008 XHG=1133 XHG=1177 YBD=3165 YBD=3154 YBD=3163 YBD=3153 YBD=3156 YBD=3155 YBD=3154 YHG=3127>panying YHG=3127>description YHG=3125>of YHG=3126>preparation) YHG=3126>in YHG=3126>distilled YHG=3130>water YHG=3127>is YHG=3125>made " XBD=152 XBD=287 XBD=342 XBD=420 XBD=518 XBD=647 XBD=774 XBD=831 XBD=933 XBD=1011 XBD=1188 XBD=1245 XHG=81 XHG=169 XHG=304 XHG=359 XHG=439 XHG=535 XHG=664 XHG=792 XHG=951 XHG=1029 XHG=1205 XHG=1259 YBD=3194 YBD=3196 YBD=3194 YBD=3193 YBD=3193 YBD=3193 YHG=3167>and YHG=3166>stored YHG=3166>in YHG=3165>the YHG=3165>cold YHG=3165>under YHG=3165>toluol YHG=3174>or YHG=3165>with YHG=3165>the YHG=3165>addition YHG=3164>of YHG=3174>a " XBD=1281 XBD=1407XHG=847 XBD=1474 XBD=1586 XBD=1659 XBD=1816 XBD=2105 XBD=2168 XBD=2248 XBD=2360 XBD=2508 XHG=1339 XHG=1429 XHG=1497 XHG=1610 XHG=1682 XHG=1840 XHG=2128 XHG=2186 XHG=2270 XHG=2382 YBD=3200 YBD=3201 YBD=3193 YBD=3194 YBD=3195 YBD=3203 YHG=3174>can YHG=3165>be YHG=3164>done YHG=3165>by YHG=3165>specific YHG=3161>neutralisation YHG=3165>of YHG=3165>the YHG=3166>MCP YHG=3167>activity

" XBD=2509 XBD=170 XHG=814 XBD=399 XBD=458 XBD=501 XBD=768 XBD=971 XBD=1019 XBD=1233 XBD=1285 XBD=1520 XBD=1658 XBD=1773 XBD=1848 XBD=1957 XBD=2125 XBD=2177 XBD=2293 XBD=2455 XHG=79 XHG=190 XHG=420 XHG=479 XHG=520 XHG=991 XHG=1039 XHG=1254 XHG=1340 XHG=1535 XHG=1674 XHG=1787 XHG=1863 XHG=1973 XHG=2140 XHG=2193 XHG=2306 XHG=2466 YBD=3233 YBD=3233 YBD=3242 YBD=3232 YBD=3231 YBD=3237 YBD=3239 YBD=3232 YBD=3233 YBD=3242 YBD=3234 YHG=3205>little YHG=3205>chloroform YHG=3214>as YHG=3214>a YHG=3204>preservative. YHG=3204>Solution YHG=3204>is YHG=3204>facilitated YHG=3203>if YHG=3204>described YHG=3205>above, YHG=3204>using YHG=3205>the YHG=3213>same YHG=3204>antisera YHG=3214>as YHG=3205>those YHG=3206>supplied YHG=3206>for " XBD=2258 |>" XBD=1401 XBD=1661 XBD=1744 XBD=1852 XBD=1980 XBD=2100 XBD=2516 XHG=1339 XHG=1414 XHG=1675 XHG=1760 XHG=1865 XHG=2027 XHG=2112 XHG=2269 YBD=3278 YBD=3270 YBD=3271 YBD=3274 YHG=3243>the YHG=3242>viscosimetric YHG=3245>test YHG=3243>(vide YHG=3242>infra). YHG=3243>The YHG=3243>method YHG=3244>recommended’ XBD=141 XBD=273 XBD=322 XBD=466 XBD=522 XBD=564 XBD=645 XBD=701 XBD=908 XBD=981 XBD=1065 XBD=1189 XBD=1281 XHG=79 XHG=160 XHG=293 XHG=340 XHG=485 XHG=542 XHG=586 XHG=665 XHG=723 XHG=926 XHG=1001 XHG=1084 XHG=1210 YBD=3272 YBD=3281 YBD=3272 YBD=3271 YBD=3270 YHG=3244>the YHG=3244>bottle YHG=3244>is YHG=3244>placed YHG=3244>in YHG=3253>a YHG=3243>370 YHG=3243>C. YHG=3243>incubator YHG=3243>for YHG=3244>2-3 YHG=3243>hours YHG=3243>and " XBD=2113 XBD=1393 XBD=1604 XBD=1789 XBD=1836 XBD=1893 XBD=2043 XHG=1339 XHG=1414 XHG=1623 XHG=1808 XHG=1855 XHG=1911 XHG=2059 YBD=3316 YBD=3317 YBD=3310 YBD=3309 YHG=3283>for YHG=3281>diagnostic YHG=3290>purposes YHG=3282>is YHG=3291>as YHG=3281>follows YHG=3292>:XBD=214 XHG=857 XBD=478 XBD=601 XBD=784 XBD=847 XBD=919 XBD=1146 XBD=1280 XHG=80 XHG=235 XHG=527 XHG=623 XHG=807 XHG=942YBD=3310 XHG=1168 YBD=3318 YBD=3310 YBD=3317 YBD=3309 YBD=3309 YHG=3282>shaken YHG=3283>occasionally. YHG=3282>The YHG=3281>strength YHG=3280>of YHG=3281>the YHG=3278>solution YHG=3282>deter- is |>" XBD=1458 XBD=1639 XBD=1702 XBD=1906 XBD=2092 XBD=2160 XBD=2301 XBD=2459 XBD=2508 XBD=1558 XHG=1384 XHG=1579 XHG=1663 XHG=1720 XHG=1928 XHG=2115 XHG=2172 XHG=2320 XHG=2477 XHG=1482 YBD=3355 YBD=3349 YBD=3350 YBD=3351 YBD=3349 YHG=3321>Mix YHG=3320>ml. YHG=3320>of’ YHG=3320>ascending YHG=3320>dilutions YHG=3320>of YHG=3320>wound YHG=3322>exudate YHG=3330>or YHG=3321>0-25 " XBD=199 XBD=266 XBD=525 XBD=615 XBD=720 XBD=761 XBD=950 XBD=1190 XBD=1281 XHG=79 XHG=217 XHG=286 XHG=544 XHG=633 XHG=739 XHG=781 XHG=967 XHG=1210 YBD=3348 YBD=3355 YBD=3358 YBD=3350 YBD=3349 YBD=3348 YBD=3356 YBD=3348 YHG=3320>mined YHG=3322>by YHG=3322>comparative YHG=3323>test YHG=3321>with YHG=3330>a YHG=3319>standard YHG=3320>preparation YHG=3320>and to " XBD=1471 XBD=1627 XBD=1730 XBD=1828 XBD=1906 XBD=1967 XBD=2006 XBD=2070 XBD=2127 XBD=2296 XBD=2350 XBD=2463 XBD=2510 XHG=1339 XHG=1491 XHG=1646 XHG=1752 XHG=1847 XHG=1928 XHG=1983 XHG=2029 XHG=2088 XHG=2148 XHG=2314 XHG=2368 XHG=2478 YBD=3387 YBD=3388 YBD=3389 YBD=3390 YHG=3360>muscle YHG=3361>extract YHG=3359>with YHG=3360>0-25 YHG=3360>ml. YHG=3369>a YHG=3360>1 YHG=3361>10 YHG=3360>dilution YHG=3360>in YHG=3361>saline YHG=3359>of |>" XBD=109 XBD=266 XBD=375 XBD=471 XBD=680 XHG=80 XHG=127 XHG=284 XHG=394 XHG=673 YBD=3387 YBD=3395 YBD=3387 YBD=3388 0-25%. YHG=3360>is YHG=3359>usually YHG=3359>from YHG=3361>0-15 YHG=3382>· : tSet-MM.―Normal XBD=565 XBD=696 XBD=776 XBD=881 XBD=983 XBD=1048 XBD=1105 XBD=1189 XBD=1234 XBD=1282 XHG=464 XHG=580 XHG=712 XHG=793 XHG=897 XHG=1000 XHG=1065 XHG=1123 XHG=1206 XHG=1253 YBD=3427 YBD=3426 YBD=3425 YBD=3432 YBD=3425 YHG=3399>horse YHG=3407>serum YHG=3398>has YHG=3398>been YHG=3397>used YHG=3398>by YHG=3406>us YHG=3397>but YHG=3398>it YHG=3398>is " XBD=2506 XBD=1401 XBD=1645 XBD=1825 XBD=1980 XBD=2062 XBD=2360 XHG=1339 XHG=1421 XHG=1665 XHG=1871 XHG=2001 XHG=2082 XHG=2371 YBD=3426 YBD=3435 YBD=3427 YBD=3428 YBD=3429 YHG=3398>appropriate YHG=3399>antisera. YHG=3399>Allow YHG=3399>the YHG=3399>exudate-serum YHG=3400>-mixture " XBD=1287 XBD=248 XBD=347 XBD=484 XBD=618 XBD=752 XBD=815 XBD=972 XBD=1149 XBD=1233 XHG=81 XHG=267 XHG=365 XHG=504 XHG=636 XHG=771 XHG=992 XHG=1196 XHG=1253 YBD=3472 YBD=3465 YBD=3464 YBD=3473 YBD=3465 YBD=3464 YBD=3467 YHG=3435>probable YHG=3437>that YHG=3438>rabbit YHG=3447>serum YHG=3438>would YHG=3437>be YHG=3437>equally YHG=3436>suitable. YHG=3437>It YHG=3437>is XBD=1377XHG=834 XBD=1503 XBD=1559 XBD=1673 XBD=1925 XBD=1995 XBD=2056 XBD=2171 XBD=2275 XBD=2360 XBD=2393 XBD=2462 XBD=2508 XHG=1338 XHG=1396 XHG=1521 XHG=1576 XHG=1691 XHG=1941 XHG=2017 XHG=2074 XHG=2189 XHG=2292 XHG=2380 XHG=2412 XHG=2477 YBD=3474 YBD=3466 YBD=3473 YBD=3468 YHG=3440>to YHG=3437>stand YHG=3439>at YHG=3446>room YHG=3440>temperature YHG=3438>for YHG=3439>]5 YHG=3439>min., YHG=3439>then YHG=3439>add YHG=3440>1 YHG=3439>ml. YHG=3437>of

,

359

" XBD=2569 XBD=1484 XBD=1590 XBD=1652 XBD=1873 XBD=1976 XBD=2040 XBD=2210 XBD=2268 XBD=2333 XBD=2437 XBD=2491 XHG=1411 XHG=1505 XHG=1611 XHG=1668 XHG=1892 XHG=1998 XHG=2060 XHG=2231 XHG=2291 XHG=2353 XHG=2460 XHG=2508 YBD=234 YBD=240 YBD=231 YBD=233 YBD=232 YHG=206>The YHG=207>time YHG=205>of YHG=204>incubation YHG=213>may YHG=203>be YHG=203>reduced YHG=205>to YHG=204>20 YHG=203>min. YHG=203>if YHG=203>the XBD=296 XHG=894 XBD=449 XBD=501 XBD=602 XBD=706 XBD=793 XBD=879 XBD=948 XBD=1256 XBD=1308 XHG=152 XHG=309 XHG=465 XHG=517 XHG=618 XHG=721 XHG=808 XHG=963YBD=235 XHG=1270 YBD=239 YBD=237 YBD=236 YBD=244 YBD=235 YHG=210>substrate YHG=209>mixture YHG=211>to YHG=208>each YHG=208>tube YHG=208>and YHG=210>test YHG=207>for YHG=207>clot-prevention YHG=206>in " XBD=1544 XBD=1624 XBD=1731 XBD=1863 XBD=1918 XBD=1996 XBD=2081 XBD=2183 XBD=2323 XBD=2410 XHG=1366 XHG=1561 XHG=1642 XHG=1748 XHG=1880 XHG=1935 XHG=2013 XHG=2097 XHG=2199 XHG=2338 XHG=2426 YBD=274 YBD=273 YBD=270 YBD=271 YBD=272 YHG=246>substrate YHG=246>has YHG=245>been YHG=244>added YHG=247>to YHG=243>the YHG=243>last YHG=243>tube YHG=242>within YHG=243>this YHG=243>time. " XBD=2523 XBD=199 XHG=701 XBD=393 XBD=627 XBD=687 XBD=845 XBD=1013 XBD=1080 XBD=1253 XBD=1311 XHG=152 XHG=213 XHG=435 XHG=645 XHG=864YBD=282 XHG=1032 XHG=1098 XHG=1271 YBD=277 YBD=284 YBD=276 YBD=274 YBD=282 YBD=282 YBD=274 YHG=251>the YHG=249>u,,3ual.way. YHG=247>Inhibition YHG=246>of YHG=256>enzyme YHG=246>activity YHG=246>bv one YHG=255>any YHG=245>of

" XBD=1309 XBD=198 XHG=815 XBD=347 XBD=401 XBD=605 XBD=712 XBD=794 XBD=964 XBD=1066 XBD=1217 XHG=151 XHG=215 XHG=365 XHG=421 XHG=624 XHG=732 XHG=984YBD=321 XHG=1083 XHG=1237 YBD=317 YBD=316 YBD=315 YBD=323 YBD=314 YBD=313 YBD=313 YBD=313YHG=285>activity YHG=289>the YHG=288>antisera YHG=296>as YHG=286>compared YHG=285>with YHG=286>the YHG=275>of YHG=275>control YHG=288>test a

EXAMPLE

" XBD=271 XBD=460 XBD=643 XBD=726 XBD=868 XBD=929 XBD=1007 XBD=1306 XHG=150 XHG=283 XHG=474 XHG=665 XHG=748 XHG=890 XHG=947 XHG=1028 YBD=357 YBD=354 YBD=353 YBD=352 YBD=352 YBD=360 YHG=327>without YHG=324>antiserum YHG=324>indicates YHG=324>the YHG=333>source YHG=323>of YHG=324>the YHG=323>hyaluronidase. |>" " XBD=1776 XBD=1870 XBD=2002 XBD=2116 XBD=2280 XBD=2362 XBD=2570 XBD=1776 XHG=1368 XHG=1825 XHG=1886 XHG=2072 XHG=2132 XHG=2317 XHG=2376 XHG=1769 YBD=376 YBD=374 YBD=377 YBD=373 YBD=375 YBD=376 YHG=351>Antiserum.... YHG=344>Cl. YHG=349>welchii YHG=348>OZ. YHG=348>septic’U1n YHG=348>Ct. YHG=348>cedematiens YHG=369>. " XBD=1308 XBD=240 XBD=317 XBD=591 XBD=693 XBD=753 XBD=833 XBD=1044 XHG=184 XHG=254 XHG=333 XHG=610 XHG=715 XHG=853 XHG=1064 XHG=1249 YBD=394 YBD=398 YBD=392 YBD=391 YBD=391 YBD=391 YHG=366>For YHG=365>this YHG=364>neutralisation YHG=366>test, YHG=373>as YHG=363>the YHG=363>antiserum YHG=363>dilutions YHG=372>are " XBD=1229 XBD=1462XHG=773 XBD=1641 XBD=1777 XBD=1941 XBD=2207 XBD=2473 XHG=2436 XHG=1369 XHG=1477 XHG=1647 XHG=1907 XHG=2174 YBD=406 YBD=410 YBD=405 YBD=404 YHG=381>Mean YHG=381>flow-time YHG=368>(sec.).. YHG=380>32 YHG=379>72 YHG=380>33

" XBD=1308 XBD=232 XBD=276 XBD=395 XBD=471 XBD=663 XBD=830 XBD=876 XBD=1060 XBD=1222 XHG=150 XHG=243 XHG=287 XHG=412 XHG=485 XHG=678 XHG=846 XHG=890 XHG=1074 XHG=1238 YBD=435 YBD=433 YBD=439 YBD=432 YBD=431 YBD=430 YBD=439 YBD=438 YHG=406>made YHG=405>in YHG=404>saline, YHG=403>the YHG=403>substrate YHG=403>mixture YHG=403>is YHG=402>prepared YHG=403>without YHG=411>any " XBD=1909 XBD=1981 XBD=2032 XBD=2181 XBD=2253 XBD=2299 XHG=2399 XHG=1369 XHG=1923 XHG=1994 XHG=2080 XHG=2196 XHG=2267 YBD=462 YBD=461 YBD=457 YBD=456 YBD=460 YHG=432>Antiserum YHG=432>Gp. YHG=432>A. YHG=431>Strep. YHG=430>Gp. YHG=430>C. YHG=430>Staph. . Strep. "" XBD=2510 XBD=235 XBD=321 XBD=445 XBD=608 XBD=677 XBD=763 XBD=807 XBD=925 XBD=977 XBD=1144 XBD=1293 XHG=150 XHG=249 XHG=336 XHG=460 XHG=622 XHG=692 XHG=779 XHG=820 XHG=993 XHG=1160 YBD=474 YBD=480 YBD=472 YBD=470 YBD=469 YBD=469 YBD=477 YHG=445>saline YHG=444>(i.e., YHG=452>serum YHG=442>dilution YHG=440>for YHG=442>this YHG=442>is YHG=441>made YHG=442>in YHG=441>distilled YHG=442>water). XBD=1465XHG=940 XBD=1942 XBD=2208 XBD=2472 XHG=1370 XHG=1909 XHG=2173 XHG=2435 YBD=487 YBD=486 YHG=462>Mean YHG=464>32 YHG=462>31 YHG=462>35 |>" XBD=356 XBD=457 XBD=1116 XBD=1191 XBD=908 XHG=251 XHG=379 XHG=931 XHG=1141 XHG=478 YBD=552 YBD=549 YBD=549 YHG=529>TESTS YHG=528>FOR YHG=526>ACTIVITY YHG=527>VISCOSITY-REDUCING YHG=526>OF " XBD=1458 XBD=1547 XBD=1700 XBD=1747 XBD=1794 XBD=1905 XBD=2003 XBD=2105 XBD=2175 XBD=2359 XBD=2571 XHG=1414 XHG=1477 XHG=1567 XHG=1719 XHG=1766 XHG=1814 XHG=1923 XHG=2022 XHG=2125 XHG=2195 XHG=2379 YBD=551 YBD=559 YBD=550 YHG=522>In YHG=522>this YHG=523>sample YHG=523>it YHG=523>is YHG=523>clear YHG=523>that YHG=523>only YHG=522>CZ. YHG=522>septicunt YHG=521>antiserum " XBD=1028 XBD=731 XBD=811 XBD=881 XHG=394 XHG=764 XHG=907 YBD=597 YBD=590 YBD=595 YHG=566>HYALURONIDASE YHG=561>(C. YHG=567>W. YHG=561>HALE) XBD=1433XHG=833 XBD=1659 XBD=1734 XBD=1892 XBD=1977 XBD=2162 XBD=2443 XBD=2496 XBD=2571 XHG=1369 XHG=1446 XHG=1673 XHG=1748 XHG=1907 XHG=1991 XHG=2176 XHG=2458 XHG=2510 YBD=590 YBD=598 YBD=591 YBD=599 YBD=589 YHG=561>has YHG=562>neutralised YHG=562>the YHG=572>enzyme YHG=563>and YHG=562>therefore YHG=561>hyaluronidase YHG=561>in YHG=561>the " XBD=1498 XBD=1670 XBD=1720 XBD=1831 XBD=1901 XHG=1368 XHG=1518 XHG=1691 XHG=1739 XHG=1852 XHG=1923 YBD=629 YBD=630 YBD=631 YBD=639 YHG=601>wound YHG=601>exudate YHG=602>is YHG=601>from YHG=602>Cl. YHG=603>septicum. " XBD=2095 XBD=262 XHG=873 XBD=437 XBD=526 XBD=592 XBD=821 XBD=950 XBD=1055 XBD=1109 XBD=1310 XHG=186 XHG=273 XHG=449 XHG=546 XHG=615 XHG=973YBD=643 XHG=1076 XHG=1131 YBD=646 YBD=645 YBD=642 YBD=642 YBD=642 YBD=650 YHG=616>Two YHG=613>methods YHG=613>will YHG=613>be YHG=613>described. YHG=613>The YHG=612>first YHG=613>is YHG=612>designed " XBD=193 XBD=267 XBD=324 XBD=362 XBD=610 XBD=754 XBD=944 XBD=1041 XBD=1310 XHG=147 XHG=209 XHG=285 XHG=339 XHG=381 XHG=631 XHG=776 XHG=963 XHG=1060 YBD=686 YBD=684 YBD=683 YBD=689 YBD=682 YBD=690 YBD=682 YBD=683 YHG=657>for YHG=663>use YHG=655>in YHG=663>a YHG=652>laboratory, YHG=653>where YHG=652>pipettes YHG=652>and " XBD=2569 XBD=1498 XHG=2214 XBD=1663 XBD=1843 XBD=1939 XBD=2009 XBD=2127 XBD=2191 XHG=1420 YBD=701 XHG=1523 XHG=1686 XHG=1867 XHG=1962 XHG=2032 XHG=2149 YBD=693 YHG=651>water-baths YBD=694 YBD=695 YHG=663>The YHG=663>second YHG=663>method YHG=673>can YHG=665>be YHG=664>used YHG=664>in YHG=663>casualty-clearing " XBD=195 XBD=396 XBD=492 XBD=681 XBD=768 XBD=983 XBD=1211 XHG=147 XHG=213 XHG=417 XHG=511 XHG=701 XHG=1004 XHG=1232 YBD=726 YBD=728 YBD=721 YBD=729 YBD=729 YBD=722 YHG=704>are YHG=692>available, YHG=691>and YHG=691>requires YHG=692>the YHG=691>following YHG=691>apparatus YHG=692>and " XBD=1310 XBD=1536XHG=790 XBD=1594 XBD=1721 XBD=1861 XBD=1998 XBD=2191 XBD=2469 XBD=2569 XHG=1366 XHG=1554 XHG=1611 XHG=1736 XHG=1876 XHG=2014 XHG=2206 XHG=2488 YBD=732 YBD=740 YBD=733 YBD=741 YBD=739 YBD=738 YHG=698>stations YHG=712>or YHG=702>other YHG=703>places YHG=704>where YHG=704>pipettes, YHG=703>water-baths, YHG=703>&c., XBD=363 XHG=145 :"" XBD=2568 XBD=1433 XBD=1525 XBD=1690 XBD=1910 XBD=1974 XBD=2104 XBD=2187 XBD=2347 XBD=2407 XHG=1370YBD=769 XHG=1454 XHG=1544 XHG=1710 XHG=1932 XHG=1993 XHG=2120 XHG=2208 XHG=2368 XHG=2426 YBD=779YHG=731>reagents YBD=778 YBD=771 YBD=781 YBD=772 YBD=773 YHG=750>are YHG=742>not YHG=741>readily YHG=742>available, YHG=752>or YHG=743>when YHG=743>the YHG=743>supply YHG=743>of YHG=743>wound

flow-time

,

" XBD=375 XBD=549 XHG=178 XHG=387 YBD=809 YBD=812 YHG=784>Thoma-Zeiss YHG=783>leucocyte " XBD=695 XBD=1536XHG=563 XBD=1593 XBD=1779 XBD=1906 XBD=2132 XBD=2190 XBD=2278 XBD=2407 XBD=2571 XHG=1370YBD=811 XHG=1560 XHG=1616 XHG=1828 XHG=1930 XHG=2156 XHG=2212 XHG=2303 XHG=2432 YBD=810YHG=783>pipette. YBD=811 YBD=819 YBD=812 YBD=820 YHG=781>exudate YHG=781>is YHG=780>limited. YHG=781>The YHG=781>technique YHG=782>is YHG=782>the YHG=791>same YHG=781>except " XBD=521 XBD=361 XHG=488 XBD=408 XBD=475 XHG=178 YBD=837 XHG=373 XHG=423 YBD=839 YHG=813>C. YBD=838 YHG=813>Water-bath YHG=813>at YHG=813>37°

" " " " " " "

XBD=2026 XBD=1429 XBD=1516 XBD=1729 XHG=1370 XHG=1448 XHG=1537 XHG=1748 YBD=850 YBD=857 YBD=851 YHG=820>for YHG=820>the YHG=820>following YHG=819>modifications some XBD=201 XHG=854 XBD=288 XBD=470 XBD=689 XBD=839 XBD=956 XHG=177 XHG=211 XHG=300 XHG=594 XHG=703 YBD=871 YBD=872 YBD=867 YBD=868 YHG=844>A YHG=844>large YHG=842>test-tube YHG=843>small YHG=843>dilution YHG=843>tubes. ; XBD=207 XBD=268 XBD=342 XBD=372 XBD=439 XBD=632 XBD=796 XHG=178 XHG=221 XHG=283 XHG=357 XHG=387 XHG=453 XHG=647 YBD=897 YBD=898 YBD=896 YBD=900 YHG=874>10 YHG=872>and YHG=873>1 YHG=872>ml. YHG=862>graduated YHG=872>pipettes. XBD=1487 XBD=1724 XBD=1831 XBD=1900 XBD=2085 XBD=2158 XBD=2204 XBD=2358 XBD=2422 XBD=2508 XBD=2575 XHG=1414 XHG=1511 XHG=1748 XHG=1856 XHG=1924 XHG=2109 XHG=2183 XHG=2229 XHG=2382 XHG=2446 XHG=2534 YBD=901 YBD=909 YBD=902 YBD=910 YBD=911 YBD=903 YHG=873>The YHG=873>water-bath YHG=882>may YHG=873>be YHG=874>replaced YHG=874>by YHG=884>a YHG=875>beaker YHG=885>or YHG=875>jug YHG=874>of XBD=199 XBD=291 XBD=363 XBD=397 XBD=511 XBD=604 XBD=734 XBD=847 XBD=1042 XBD=1077 XBD=1291 XHG=176 XHG=211 XHG=303 XHG=377 XHG=407 XHG=524 XHG=617 XHG=864 XHG=1057 XHG=1090 YBD=927 YBD=926 YBD=932 YBD=931 YBD=927 YBD=931 YHG=904>A YHG=902>clock YHG=902>and YHG=907>a YHG=901>watch YHG=901>with YHG=902>second YHG=902>hand, YHG=902>preferably YHG=908>a YHG=902>stop-watch. XBD=1479XHG=749 XBD=1508 XBD=1600 XBD=1773 XBD=1877 XBD=1971 XBD=2090 XBD=2138 XBD=2258 XBD=2329 XBD=2381 XBD=2492 XBD=2570 XHG=1369 XHG=1494 XHG=1521 XHG=1610 XHG=1783 XHG=1886 XHG=1981 XHG=2099 XHG=2148 XHG=2270 XHG=2344 XHG=2396 XHG=2502 YBD=950 YBD=941 YBD=942 YBD=944 YBD=951 YHG=913>about YHG=914>1 YHG=913>pint YHG=913>capacity YHG=913>filled YHG=913>with YHG=916>water YHG=916>at YHG=914>about YHG=915>45° YHG=915>C. YHG=915>(113° YHG=915>F.). XBD=273 XBD=415 XBD=464 XBD=597 XBD=661 XHG=177 XHG=284 XHG=429 XHG=476 XHG=611 YBD=956 YHG=931>Buffer YHG=931>solution YHG=936>as YHG=930>follows YHG=938>:-

:-

.

" XBD=1442 XBD=1584 XBD=1749 XBD=1791 XBD=1950 XBD=2001 XBD=2119 XBD=2186 XBD=2220 XBD=2299 XBD=2465 XBD=2572 XHG=1369 XHG=1454 XHG=1596 XHG=1763 XHG=1805 XHG=1962 XHG=2015 XHG=2131 XHG=2199 XHG=2232 XHG=2311 XHG=2480 YBD=981 YBD=982 YHG=953>The YHG=953>wound YHG=953>exudate YHG=953>is YHG=953>allowed YHG=955>to YHG=953>stand YHG=953>for YHG=963>a YHG=953>few YHG=953>minutes YHG=953>after |>|>" " XBD=365 XBD=554 XBD=1022 XBD=1158 XBD=1201 XBD=755 XHG=211 XHG=377 XHG=994 XHG=1070 XHG=1172 XHG=571 YBD=993 YBD=998 YBD=995 YBD=995 YBD=999 YBD=997 YHG=968>Disodium YHG=967>phosphate YHG=987>.. YHG=970>13’75 YHG=977>g. YHG=968>(Na2HP04 anhydrous)

" XBD=2572 XBD=295 XHG=207 XBD=1021 XBD=1201 XBD=1552 XBD=1604 XBD=1745 XBD=1790 XBD=1844 XBD=2051 XBD=2171 XBD=2258 XBD=2302 XBD=2484 XHG=307YBD=1022 XHG=1088 XHG=1368 XHG=1567 XHG=1622 XHG=1761 XHG=1806 XHG=1860 XHG=2098 XHG=2187 XHG=2273 XHG=2317 XHG=2500 YBD=1024 YBD=1029YHG=998>Citric YBD=1020 YBD=1021 YBD=1022 YHG=998>acid YHG=1000>202g. YHG=992>collection YHG=995>to YHG=992>enable YHG=992>it YHG=994>to YHG=992>coagulate. YHG=992>The YHG=987>clot YHG=993>is YHG=993>removed YHG=993>and

" XBD=288 XHG=392 YBD=1052 YBD=1054 YHG=1027>NaCl YHG=1045>............ g. " XBD=1021 XBD=1430XHG=210 XBD=1646 XBD=1756 XBD=1808 XBD=1952 XBD=2023 XBD=2086 XBD=2217 XBD=2251 XBD=2289 XBD=2416 XBD=2486 XBD=2571 XHG=1368 XHG=1453 XHG=1670 XHG=1780 XHG=1832 XHG=1975 XHG=2048 XHG=2110 XHG=2236 XHG=2269 XHG=2313 XHG=2439 XHG=2509 YBD=1068 YBD=1069 YBD=1059 YBD=1044 YBD=1060 YHG=1032>remaining YHG=1032>fluid YHG=1031>is YHG=1032>drawn YHG=1041>up YHG=1034>to YHG=1031>the YHG=1033>1 YHG=1031>&dquo; YHG=1031>mark YHG=1041>on YHG=1032>the &dquo; " XBD=1194 XBD=311 XHG=321 XBD=354 XBD=1105 XHG=209 XHG=1090 XHG=1120 YBD=1082 YBD=1081 YBD=1083 YBD=1084 YHG=1057>Water YHG=1057>to YHG=1060>1 YHG=1059>litre ...........

" XBD=1613 XBD=1782 XBD=1994 XBD=2192 XBD=2244 XBD=2354 XBD=2499 XHG=1369 XHG=1635 XHG=1828 XHG=2016 XHG=2215 XHG=2267 XHG=2377 XHG=2521 YBD=1100 YBD=1109 YHG=1071>Thoma-Zeiss YHG=1071>pipette. YHG=1071>Buffered YHG=1072>substrate YHG=1071>is YHG=1071>then YHG=1071>drawn YHG=1081>up " XBD=2569 XBD=314 XHG=502 XBD=435 XBD=487 XBD=659 XBD=1004 XBD=1164 XHG=176 XHG=325 XHG=449 XHG=786YBD=1123 XHG=1016 YBD=1120 YBD=1119 YBD=1124 YBD=1121 YHG=1093>Reaction YHG=1093>should YHG=1094>be YHG=1094>pH’==7-0. YHG=1087>dyaluronate YHG=1095>solution. " XBD=2572 XBD=1408 XBD=1507 XBD=1672 XBD=1800 XBD=1855 XBD=1894 XBD=2029 XBD=2206 XBD=2289 XBD=2448 XHG=1369 XHG=1430 XHG=1530 XHG=1693 XHG=1818 XHG=1873 XHG=1917 XHG=2052 XHG=2228 XHG=2310 XHG=2469 YBD=1138 YBD=1149 YBD=1123 YBD=1145 YBD=1147 YBD=1139 YBD=1148 YBD=1140 YHG=1114>to YHG=1111>well YHG=1111>beyond YHG=1112>11 YHG=1111>&dquo; YHG=1110>mark, YHG=1111>twisting YHG=1111>the YHG=1111>pipette YHG=1111>while &dquo; |>" " XBD=213 XBD=273 XBD=442 XBD=635 XBD=702 XBD=940 XBD=1311 XBD=1222 XHG=174 XHG=237 XHG=294 XHG=467 XHG=667 XHG=733 XHG=1250 XHG=972 YBD=1155 YBD=1159 YBD=1160 YBD=1156 YBD=1157 YBD=1162 YBD=1162 YHG=1132>To YHG=1131>be YHG=1131>prepared YHG=1132>according YHG=1132>to YHG=1132>instructions YHG=1133>dry YHG=1133>accompanying " XBD=783 XBD=1476XHG=621 XBD=1583 XBD=1723 XBD=1881 XBD=1959 XBD=2061 XBD=2204 XBD=2315 XBD=2359 XBD=2462 XBD=2573 XHG=1371 XHG=1491 XHG=1600 XHG=1738 XHG=1927 XHG=1973 XHG=2076 XHG=2219 XHG=2329 XHG=2374 XHG=2476 YBD=1186 YBD=1187 YBD=1179 YHG=1150>doing YHG=1152>so’to YHG=1144>ensure YHG=1151>mixing. YHG=1151>A YHG=1150>wide YHG=1151>rubber YHG=1150>band YHG=1150>is YHG=1150>then YHG=1150>fixed " XBD=244 XBD=388 XBD=500 XBD=565 XBD=604 XHG=142 XHG=258 XHG=401 XHG=515 XHG=575 YBD=1189 YBD=1186 YBD=1190 YBD=1191 YHG=1160>sample YHG=1160>(usually YHG=1160>about YHG=1162>0 YHG=1162>% YHG=1162>solution). -5 " XBD=2572 XBD=1572 XHG=2509 XBD=1732 XBD=1814 XBD=1971 XBD=2065 XBD=2149 XBD=2281 XBD=2428 XBD=2487 XHG=1366 YBD=1219 XHG=1584 XHG=1753 XHG=1836 XHG=1995 XHG=2088 XHG=2170 XHG=2302 XHG=2449 YBD=1225 YHG=1190>the YBD=1227 YBD=1218 YHG=1190>lengthwise YHG=1190>-around YHG=1190>pipette YHG=1190>and YHG=1190>whole YHG=1190>placed YHG=1190>in " YBD=1265 YHG=1240>TE(’HNIQUE " XBD=818 XBD=1431XHG=604 XBD=1489 XBD=1611 XBD=1737 XBD=1808 XBD=1869 XBD=1970 XHG=1368 XHG=1449 XHG=1502 XHG=1628 XHG=1753 XHG=1827 XHG=1886 YBD=1266 YBD=1257 YBD=1258 YHG=1230>jug YHG=1228>of YHG=1239>warm YHG=1232>water YHG=1230>for YHG=1230>20 YHG=1229>min. " XBD=1487 XBD=1639 XBD=1684 XBD=1864 XBD=1970 XBD=2046 XBD=2170 XBD=2292 XBD=2380 XBD=2517 XHG=1414 XHG=1502 XHG=1655 XHG=1699 XHG=1880 XHG=1985 XHG=2060 XHG=2185 XHG=2308 XHG=2396 XHG=2533 YBD=1306 YBD=1297 YBD=1296 YHG=1269>The YHG=1269>pipette YHG=1270>is YHG=1269>removed YHG=1268>from YHG=1268>the YHG=1277>warm YHG=1271>water YHG=1268>and YHG=1268>cooled YHG=1271>to " XBD=2572 XBD=242 XHG=878 XBD=388 XBD=443 XBD=516 XBD=648 XBD=817 XBD=862 XBD=996 XBD=1097 XBD=1136 XBD=1309 XHG=178 XHG=257 XHG=405 XHG=457 XHG=533 XHG=664 XHG=833 XHG=1011 XHG=1115 XHG=1153 YBD=1313 YBD=1314 YBD=1315 YBD=1316 YHG=1286>One YHG=1285>volume YHG=1285>of YHG=1287>the YHG=1287>buffer YHG=1286>solution YHG=1287>is YHG=1287>mixed YHG=1287>with YHG=1289>4 YHG=1287>volumes " XBD=1468 XBD=1737 XBD=1940 XBD=2023 XBD=2289 XBD=2383 XBD=2573 XHG=1369 XHG=1490 XHG=1784 XHG=1961 XHG=2043 XHG=2310 XHG=2403 YBD=1345 YBD=1336 YBD=1343 YHG=1317>room YHG=1311>temperature. YHG=1309>Remove YHG=1308>the YHG=1307>rubber YHG=1308>and YHG=1307>carefully band " XBD=174 XBD=401 XBD=571 XBD=663 XBD=785 XBD=841 XBD=883 XBD=1113 XBD=1171 XBD=1252 XBD=1308 XHG=141 XHG=188 XHG=420 XHG=591 XHG=682 XHG=805 XHG=862 XHG=901 XHG=1132 XHG=1193 XHG=1272 YBD=1353 YBD=1360 YBD=1355 YBD=1354 YBD=1356 YBD=1356 YHG=1324>of YHG=1324>hyaluronate YHG=1326>solution YHG=1326>and YHG=1326>stood YHG=1327>in YHG=1336>a YHG=1327>water-bath YHG=1329>at YHG=1327>37° " XBD=2574 XBD=1412 XHG=2510 XBD=1496 XBD=1646 XBD=1765 XBD=1885 XBD=1995 XBD=2045 XBD=2154 XBD=2322 XBD=2383 XBD=2485 XHG=1369 YBD=1374 XHG=1435 XHG=1519 XHG=1668 XHG=1812 XHG=1907 XHG=2016 XHG=2067 XHG=2177 XHG=2344 XHG=2406 YBD=1376 YHG=1328>C. YBD=1375 YHG=1348>fit YHG=1348>the YHG=1348>mouth YHG=1348>tube. YHG=1348>The YHG=1347>fluid YHG=1347>is YHG=1347>then YHG=1346>allowed YHG=1349>to YHG=1345>flow YHG=1349>out " XBD=302 XBD=368 XBD=445 XBD=569 XBD=661 XBD=700 XBD=817 XBD=983 XBD=1092 XBD=1189 XBD=1309 XHG=177 XHG=318 XHG=384 XHG=463 XHG=586 XHG=679 XHG=716 XHG=833 XHG=1006 XHG=1117 XHG=1211 YBD=1401 YBD=1393 YBD=1394 YBD=1395 YBD=1395 YBD=1394 YBD=1403 YHG=1364>Pipette YHG=1365>0.1 YHG=1365>ml. YHG=1366>saline YHG=1366>into YHG=1375>a YHG=1365>small YHG=1366>dilution YHG=1367>tube YHG=1366>and YHG=1366>place " XBD=1445 XBD=1487 XBD=1606 XBD=1711 XBD=1876 XBD=1955 XBD=2026 XBD=2087 XBD=2162 XBD=2314 XBD=2518 XHG=1369 XHG=1465 XHG=1505 XHG=1624 XHG=1729 XHG=1895 XHG=1974 XHG=2045 XHG=2101 XHG=2179 XHG=2332 XHG=2536 YBD=1415 YBD=1423 YBD=1413 YBD=1422 YBD=1414 YHG=1387>into YHG=1396>a YHG=1387>small YHG=1388>tube YHG=1386>keeping YHG=1386>the YHG=1386>tip YHG=1385>of YHG=1385>the YHG=1385>pipette YHG=1385>immersed YHG=1386>in " XBD=2574 XBD=171 XHG=866 XBD=247 XBD=483 XBD=646 XBD=726 XBD=840 XBD=951 XBD=1078 XBD=1159 XBD=1245 XBD=1313 XHG=139 XHG=192 XHG=270 XHG=531 XHG=671 XHG=755 XHG=996YBD=1435 XHG=1105 XHG=1186 XHG=1272 YBD=1432 YBD=1431 YBD=1433 YBD=1434 YBD=1435 YBD=1434 YBD=1435 YBD=1436 YHG=1405>in YHG=1404>the YHG=1404>water-bath. YHG=1405>Leave YHG=1405>for YHG=1406>5-10 YHG=1406>min. YHG=1406>Add YHG=1407>0-9 YHG=1406>ml. YHG=1406>of " XBD=1432 XBD=1527 XBD=1611 XBD=1736 XBD=1834 XBD=1930 XBD=2022 XBD=2141 XBD=2245 XBD=2396 XBD=2574 XHG=1371 XHG=1449 XHG=1544 XHG=1631 XHG=1753 XHG=1852 XHG=1949 XHG=2069 XHG=2160 XHG=2263 XHG=2414 YBD=1455 YBD=1463 YBD=1453 YBD=1454 YHG=1427>the YHG=1426>first YHG=1426>few YHG=1427>drops YHG=1425>that YHG=1426>flow YHG=1427>out. YHG=1424>The YHG=1424>time YHG=1424>of YHG=1425>between flow "" XBD=1311 XBD=277 XBD=517 XBD=688 XBD=909 XBD=999 XBD=1088 XBD=1154 XHG=140 XHG=292 XHG=535 XHG=707 XHG=928 XHG=1015 XHG=1105 XHG=1171 YBD=1472 YBD=1479 YBD=1473 YBD=1482 YBD=1474 YBD=1473 YBD=1481 YHG=1443>buffered YHG=1442>hyaluronate YHG=1444>solution YHG=1445>(substrate) YHG=1445>and YHG=1446>mix YHG=1445>by YHG=1445>stirring XBD=1476 XBD=1532 XBD=1708 XBD=1740 XBD=1779 XBD=1919 XBD=1968 XBD=2107 XBD=2290 XBD=2372 XBD=2574 XHG=1370 XHG=1495 XHG=1593 XHG=1726 XHG=1760 XHG=1801 XHG=1939 XHG=1989 XHG=2153 XHG=2310 XHG=2392 YBD=1493 YBD=1477 YBD=1501 YBD=1492 YBD=1494 YHG=1466>the YHG=1466>11 YHG=1466>and YHG=1466>1 YHG=1465>&dquo; YHG=1464>marks YHG=1465>is YHG=1464>noted. YHG=1464>Repeat YHG=1464>the YHG=1465>flow-time &dquo; "" XBD=1311 XBD=199 XBD=348 XBD=409 XBD=498 XBD=619 XBD=721 XBD=760 XBD=955 XBD=1116 XHG=140 XHG=213 XHG=363 XHG=428 XHG=516 XHG=634 XHG=738 XHG=776 XHG=971 XHG=1157 YBD=1519 YBD=1520 YBD=1512 YBD=1513 YBD=1521 YBD=1522 YBD=1521 YHG=1482>and YHG=1483>sucking YHG=1493>up YHG=1485>and YHG=1484>down YHG=1484>with YHG=1494>a YHG=1485>leucocyte YHG=1484>pipette. YHG=1484>Holding XBD=1458 XBD=1512 XBD=1626 XBD=1712 XBD=1808 XBD=1935 XBD=2064 XBD=2182 XBD=2373 XBD=2576 XHG=1372 XHG=1473 XHG=1525 XHG=1641 XHG=1726 XHG=1822 XHG=1982 XHG=2075 XHG=2193 XHG=2387 YBD=1534 YBD=1532 YBD=1533 YBD=1541 YHG=1514>once YHG=1514>or YHG=1504>twice YHG=1506>and YHG=1504>take YHG=1514>mean. YHG=1504>This YHG=1513>mean YHG=1504>flow-time YHG=1504>compared " XBD=190 XBD=252 XBD=305 XBD=375 XBD=524 XBD=613 XBD=741 XBD=817 XBD=968 XBD=1024 XBD=1097 XBD=1209 XBD=1313 XHG=139 XHG=203 XHG=268 XHG=317 XHG=390 XHG=540 XHG=629 XHG=757 XHG=833 XHG=984 XHG=1036 XHG=1112 XHG=1226 YBD=1550 YBD=1559 YBD=1551 YBD=1560 YBD=1552 YBD=1553 YBD=1552 YBD=1558 YBD=1552 YHG=1523>tip YHG=1522>of YHG=1523>the YHG=1523>pipette YHG=1524>just YHG=1524>under YHG=1524>the YHG=1524>surface YHG=1524>of YHG=1523>the YHG=1524>fluid, YHG=1524>suck " XBD=1458 XBD=1552 XBD=1606 XBD=1677 XBD=1826 XBD=1893 XBD=1966 XBD=2112 XBD=2166 XBD=2351 XBD=2452 XBD=2574 XHG=1371 XHG=1471 XHG=1567 XHG=1616 XHG=1690 XHG=1838 XHG=1906 XHG=1980 XHG=2125 XHG=2175 XHG=2364 XHG=2465 YBD=1573 YBD=1580 YBD=1572 YHG=1545>with YHG=1544>that YHG=1545>of YHG=1545>the YHG=1544>normal YHG=1544>for YHG=1544>the YHG=1543>sample YHG=1543>of YHG=1543>substrate YHG=1544>with YHG=1543>saline " XBD=178 XBD=225 XBD=295 XBD=382 XBD=420 XBD=536 XBD=620 XBD=736 XBD=811 XBD=911 XBD=963 XBD=1055 XBD=1133 XBD=1238 XHG=139 XHG=191 XHG=238 XHG=311 XHG=401 XHG=435 XHG=550 XHG=635 XHG=750 XHG=825 XHG=925 XHG=977 XHG=1070 XHG=1146 XHG=1251 YBD=1599 YBD=1590 YBD=1574 YBD=1592 YBD=1591 YBD=1592 YBD=1592 YBD=1591 YHG=1571>up YHG=1564>to YHG=1562>the YHG=1562>&dquo; YHG=1564>mark YHG=1564>and YHG=1563>allow YHG=1563>the YHG=1563>fluid YHG=1566>to YHG=1563>flow YHG=1566>out YHG=1563>until YHG=1563>the 11 "" XBD=1313 XBD=1511 XBD=1567 XBD=1732 XBD=1859 XBD=2005 XBD=2216 XBD=2271 XBD=2386 XBD=2575 XHG=1371 XHG=1527 XHG=1579 XHG=1748 XHG=1875 XHG=2020 XHG=2233 XHG=2287 XHG=2402 YBD=1612 YBD=1611 YBD=1620 YHG=1583>instead YHG=1583>of YHG=1584>exudate YHG=1583>under YHG=1583>similar YHG=1579>conditions YHG=1585>at YHG=1592>room YHG=1586>temperaXBD=290 XHG=851 XBD=452 XBD=542 XBD=592 XBD=805 XBD=950 XBD=1038 XBD=1141 XBD=1209 XBD=1312 XHG=139 XHG=316 XHG=480 XHG=572 XHG=610 XHG=976YBD=1631 XHG=1064 XHG=1169 XHG=1233 YBD=1630 YBD=1615 YBD=1632 YBD=1631 YBD=1631 YHG=1602>meniscus YHG=1602>reaches YHG=1603>the YHG=1603>&dquo; YHG=1602>&dquo;mark. YHG=1602>Time YHG=1603>the YHG=1606>rate YHG=1602>of YHG=1603>flow " XBD=1451 XBD=1580 XBD=1662 XBD=1887 XBD=1960 XBD=2050 XBD=2247 XBD=2320 XBD=2503 XBD=2577 XHG=1371 XHG=1484 XHG=1615 XHG=1695 XHG=1921 XHG=1990 XHG=2084 XHG=2280 XHG=2353 XHG=2537 YBD=1660 YBD=1652 YBD=1651 YHG=1626>ture YHG=1623>gives YHG=1633>an YHG=1623>indication YHG=1623>the YHG=1631>presence YHG=1631>or YHG=1623>absence YHG=1622>of " XBD=277 XBD=392 XBD=484 XBD=635 XBD=820 XBD=928 XBD=991 XBD=1113 XBD=1207 XBD=1313 XHG=139 XHG=298 XHG=413 XHG=507 XHG=683 XHG=843 XHG=952 XHG=1012 XHG=1136 XHG=1229 YBD=1670 YBD=1679 YBD=1671 YBD=1672 YBD=1671 YHG=1643>between YHG=1642>these YHG=1644>two YHG=1642>points. YHG=1643>Repeat YHG=1652>once YHG=1652>or YHG=1644>twice YHG=1642>and YHG=1642>take " XBD=1652 XBD=1731 XBD=1954 XBD=2016 XBD=2097 XBD=2297 XBD=2491 XBD=2575 XHG=1371 XHG=1699 XHG=1753 XHG=1977 XHG=2035 XHG=2118 XHG=2320 XHG=2513 YBD=1700 YBD=1699 YBD=1692 YBD=1691 YBD=1690 YHG=1663>hyaluronidase. YHG=1663>A YHG=1663>shortening YHG=1662>of YHG=1662>flow-time YHG=1662>indicates YHG=1662>the " XBD=188 XBD=302 XBD=358 XBD=442 XBD=631 XBD=761 XBD=876 XBD=957 XBD=1156 XBD=1231 XBD=1312 XHG=138 XHG=205 XHG=319 XHG=382 XHG=462 XHG=679 XHG=780 XHG=896 XHG=978 XHG=1176 XHG=1251 YBD=1718 YBD=1710 YBD=1719 YBD=1711 YBD=1711 YBD=1710 YHG=1691>mean YHG=1681>of YHG=1681>the YHG=1683>readings. YHG=1682>This YHG=1683>gives YHG=1682>the YHG=1682>flow-time YHG=1682>for YHG=1682>the " XBD=1536 XBD=1593 XBD=1668 XBD=1829 XBD=1884 XBD=1961 XBD=2107 XHG=1372 XHG=1554 XHG=1606 XHG=1685 XHG=1847 XHG=1900 XHG=1977 XHG=2123 YBD=1739 YBD=1730 YBD=1731 YHG=1712>presence YHG=1702>of YHG=1705>enzyme YHG=1703>in YHG=1703>the YHG=1702>wound YHG=1702>exudate. " XBD=2285 XBD=304 XHG=611 XBD=507 XBD=592 XBD=843 XBD=1018 XBD=1070 XBD=1179 XBD=1313 XHG=137 XHG=324 XHG=554 XHG=866YBD=1758 XHG=1041 XHG=1092 XHG=1200 YBD=1751 YBD=1750 YBD=1750 YBD=1759 YBD=1750 YHG=1721>untreated YHG=1721>substrate. YHG=1722>If YHG=1722>hyaluronate YHG=1722>solution YHG=1722>is YHG=1722>kept YHG=1721>under " XBD=1313 XBD=232 XBD=288 XBD=330 XBD=567 XBD=651 XBD=854 XBD=965 XBD=1070 XBD=1136 XHG=137 XHG=253 XHG=309 XHG=350 XHG=589 XHG=673 XHG=875 XHG=987 XHG=1093 XHG=1158 YBD=1797 YBD=1789 YBD=1790 YBD=1797 YBD=1789 YHG=1760>toluol YHG=1761>in YHG=1770>a YHG=1760>refrigerator YHG=1761>the YHG=1761>flow-time YHG=1761>need YHG=1761>only YHG=1761>be XBD=1502 XHG=2532 XBD=1646 XBD=1928 XBD=2103 XBD=2187 XBD=2412 XBD=2493 XBD=2574 XHG=1424 YBD=1794 XHG=1542 XHG=1685 XHG=1966 XHG=2140 XHG=2228 XHG=2450 YBD=1801 YHG=1760>checked YHG=1765>The YHG=1773>same YHG=1764>precautions YHG=1764>should YHG=1764>be YHG=1763>observed YHG=1772>as YHG=1764>in " XBD=480 XBD=228 XBD=333 XBD=385 XHG=137 XHG=241 XHG=347 YBD=1836 YBD=1829 YBD=1828 YBD=1830 YHG=1810>every YHG=1801>week YHG=1810>or YHG=1803>two. XBD=1439XHG=400 XBD=1548 XBD=1727 XBD=1844 XBD=1931 XBD=2146 XBD=2439 XHG=1372 XHG=1464 XHG=1569 XHG=1747 XHG=1863 XHG=1953 XHG=2166 YBD=1833 YBD=1841 YBD=1834 YHG=1803>the YHG=1803>first YHG=1803>method YHG=1804>with YHG=1804>the YHG=1803>following YHG=1802>additions :ml. YBD=1868 |>" " XBD=301 XBD=494 XBD=633 XBD=798 XBD=888 XBD=923 XBD=1037 XBD=1200 XBD=1309 XBD=365 XHG=174 XHG=454 XHG=503 XHG=646 XHG=811 XHG=902 XHG=936 XHG=1051 XHG=1213 XHG=314 YBD=1876 YBD=1869 YBD=1868 YBD=1869 YBD=1867 YBD=1868 YHG=1840>Pipette YHG=1840>of YHG=1840>wound YHG=1840>exudate YHG=1840>into YHG=1849>a YHG=1840>small YHG=1841>0YHG=1840>dilution YHG=1840>tube. " XBD=1466 XBD=1587 XBD=1669 XBD=1775 XBD=1835 XBD=1929 XBD=1977 XBD=2035 XBD=2100 XBD=2289 XBD=2346 XBD=2465 XBD=2573 XHG=1418 XHG=1487 XHG=1608 XHG=1689 XHG=1795 XHG=1850 XHG=1948 XHG=1998 XHG=2056 XHG=2119 XHG=2308 XHG=2366 XHG=2485 YBD=1893 YBD=1884 YBD=1886 YBD=1885 YHG=1858>(a) YHG=1857>Since YHG=1856>the YHG=1857>time YHG=1856>of YHG=1857>flow YHG=1857>is YHG=1859>to YHG=1858>be YHG=1857>observed YHG=1860>at YHG=1866>room YHG=1860>tem|>|>" |>" " XBD=352 XBD=438 XBD=588 XBD=715 XBD=890 XBD=1093 XBD=1207 XBD=1312 XBD=509 XBD=235 XHG=249 XHG=368 XHG=527 XHG=605 XHG=732 XHG=907 XHG=1133 XHG=1224 XHG=456 XHG=117 YBD=1908 YBD=1908 YBD=1909 YBD=1907 YBD=1908 YBD=1908 YHG=1880>add YHG=1879>ml. YHG=1888>warm YHG=1880>buffered YHG=1880>substrate. YHG=1880>0-9 YHG=1879>Mix YHG=1878>with YHG=1879>When " XBD=1534 XBD=1619 XBD=1867 XBD=1944 XBD=1979 XBD=2137 XBD=2319 XBD=2380 XBD=2572 XHG=1370 XHG=1548 XHG=1632 XHG=1881 XHG=1958 XHG=1992 XHG=2149 XHG=2333 XHG=2392 YBD=1934 YBD=1924 YBD=1925 YBD=1933 YHG=1899>perature YHG=1896>and YHG=1899>temperature YHG=1896>has YHG=1906>a YHG=1897>marked YHG=1897>influence YHG=1906>on YHG=1897>viscosity, " XBD=187 XBD=251 XBD=307 XBD=340 XBD=452 XBD=535 XBD=687 XBD=753 XBD=909 XBD=996 XBD=1157 XBD=1221 XBD=1312 XHG=135 XHG=202 XHG=269 XHG=318 XHG=355 XHG=469 XHG=552 XHG=704 XHG=769 XHG=926 XHG=1012 XHG=1173 XHG=1239 YBD=1956 YBD=1955 YBD=1947 YBD=1954 YBD=1955 YBD=1946 YHG=1919>the YHG=1918>tip YHG=1919>of YHG=1928>a YHG=1919>clean YHG=1919>dry YHG=1919>pipette YHG=1919>by YHG=1918>stirring YHG=1918>and YHG=1918>sucking YHG=1927>up YHG=1917>and "|>" XBD=1448 XBD=1592 XBD=1654 XBD=1781 XBD=1860 XBD=1916 XBD=2042 XBD=2119 XBD=2271 XBD=2327 XBD=2404 XBD=2573 XHG=1369 XHG=1466 XHG=1610 XHG=1671 XHG=1797 XHG=1878 XHG=1933 XHG=2058 XHG=2135 XHG=2288 XHG=2342 XHG=2421 YBD=1963 YBD=1964 YBD=1973 YBD=1965 YBD=1974 YHG=1945>care YHG=1936>should YHG=1936>be YHG=1936>taken YHG=1939>not YHG=1940>to YHG=1946>warm YHG=1937>pipette YHG=1937>in YHG=1936>the YHG=1933>fingers " XBD=226 XBD=363 XBD=487 XBD=689 XBD=763 XBD=827 XBD=883 XBD=953 XBD=1100 XBD=1186 XBD=1332 XHG=136 XHG=238 XHG=376 XHG=533 XHG=701 XHG=775 XHG=843 XHG=893 XHG=965 XHG=1113 XHG=1199 YBD=1988 YBD=1994 YBD=1987 YBD=1995 YBD=1986 YBD=1994 YBD=1993 YBD=1988 YHG=1959>down YHG=1958>several YHG=1958>times. YHG=1958>Holding YHG=1958>the YHG=1958>tip YHG=1957>of YHG=1957>the YHG=1958>pipette YHG=1957>just YHG=1957>under " XBD=1399 XBD=1543 XBD=1604 XBD=1705 XBD=1863 XBD=1966 XBD=2045 XBD=2186 XBD=2316 XBD=2395 XBD=2509 XHG=1368 XHG=1417 XHG=1561 XHG=1622 XHG=1721 XHG=1880 XHG=1983 XHG=2061 XHG=2204 XHG=2333 XHG=2412 YBD=2003 YBD=2012 YBD=2004 YBD=2005 YHG=1975>it YHG=1975>should YHG=1975>be YHG=1975>held YHG=1976>upright YHG=1976>with YHG=1977>the YHG=1977>fingers YHG=1976>above YHG=1976>the YHG=1970>bulb. " XBD=188 XBD=339 XBD=392 XBD=469 XBD=587 XBD=689 XBD=757 XBD=817 XBD=900 XBD=993 XBD=1027 XBD=1155 XBD=1309 XHG=136 XHG=206 XHG=353 XHG=408 XHG=489 XHG=602 XHG=709 XHG=779 XHG=838 XHG=923 XHG=1008 XHG=1042 XHG=1170 YBD=2027 YBD=2030 YBD=2035 YBD=2026 YBD=2025 YBD=2008 YBD=2032 YBD=2031 YHG=1997>surfa-ce YHG=1997>of YHG=1998>the YHG=1998>fluid, YHG=1997>suck YHG=2007>up YHG=2000>to YHG=1997>the YHG=1997>&dquo; YHG=1997>&dquo; YHG=1998>mark, YHG=1996>release, 11 " XBD=1463 XBD=1592 XBD=1736 XBD=1811 XBD=2001 XBD=2076 XBD=2240 XBD=2381 XBD=2437 XHG=1416 XHG=1477 XHG=1605 XHG=1749 XHG=1824 XHG=2015 XHG=2089 XHG=2254 XHG=2394 XHG=2453 YBD=2051 YBD=2043 YBD=2052 YBD=2044 YHG=2015>(b) YHG=2015>When YHG=2015>adding YHG=2016>substrate YHG=2016>the YHG=2016>mixture YHG=2016>should YHG=2016>be YHG=2016>drawn " XBD=2575 XBD=197 XHG=833 XBD=308 XBD=379 XBD=481 XBD=536 XBD=632 XBD=710 XBD=818 XBD=895 XBD=1083 XBD=1236 XHG=136 XHG=214 XHG=320 XHG=396 XHG=498 XHG=554 XHG=647 XHG=726 XHG=912YBD=2064 XHG=1095 XHG=1246 YBD=2067 YBD=2066 YBD=2065 YBD=2065 YBD=2065 YHG=2038>and YHG=2037>allow YHG=2038>the YHG=2037>fluid YHG=2040>to YHG=2037>flow YHG=2039>out YHG=2036>until YHG=2036>the YHG=2036>meniscus YHG=2036>reaches YHG=2037>the "" XBD=1308 XBD=1418 XBD=1515 XBD=1675 XBD=1796 XBD=2020 XBD=2181 XBD=2222 XBD=2390 XBD=2448 XBD=2575 XHG=1370 XHG=1437 XHG=1533 XHG=1692 XHG=1869 XHG=2039 XHG=2199 XHG=2240 XHG=2409 XHG=2464 YBD=2091 YBD=2090 YBD=2092 YBD=2083 YBD=2084 YHG=2063>up YHG=2046>well YHG=2055>beyond YHG=2055>the YHG=2055>&dquo; YHG=2056>making YHG=2065>a YHG=2055>dilution YHG=2055>of YHG=2056>about &dquo; mark, &dquo; XBD=153 XBD=179 XBD=336 XBD=436 XBD=509 XBD=609 XBD=660 XBD=818 XBD=940 XBD=991 XBD=1083 XBD=1223 XBD=1274 XHG=137 XHG=166 XHG=192 XHG=354 XHG=449 XHG=524 XHG=623 XHG=674 XHG=832 XHG=953 XHG=1004 XHG=1096 XHG=1237 YBD=2089 YBD=2111 YBD=2105 YBD=2104 YBD=2103 YBD=2105 YBD=2103 YHG=2079>1 YHG=2077>&dquo; YHG=2080>note YHG=2077>the YHG=2077>time YHG=2077>in YHG=2076>seconds YHG=2076>taken YHG=2078>to YHG=2076>flow YHG=2074>from YHG=2076>I mark; &dquo; " XBD=1415 XBD=1472 XBD=1633 XBD=1693 XBD=1753 XBD=1822 XBD=1951 XBD=2111 XBD=2153 XBD=2239 XBD=2365 XBD=2423 XBD=2488 XBD=2577 XHG=1373 XHG=1434 XHG=1494 XHG=1652 XHG=1714 XHG=1772 XHG=1869 XHG=1972 XHG=2132 XHG=2172 XHG=2259 XHG=2384 XHG=2444 XHG=2508 YBD=2122 YBD=2124 YBD=2132 YBD=2123 YHG=2095>1 YHG=2095>13 YHG=2094>instead YHG=2093>of YHG=2096>1 YHG=2096>11. YHG=2095>This YHG=2096>enables YHG=2105>a YHG=2095>few YHG=2095>drops YHG=2098>to YHG=2095>be YHG=2104>run : "" XBD=1308 XBD=167 XBD=270 XBD=447 XBD=571 XBD=656 XBD=825 XBD=969 XBD=1031 XBD=1132 XBD=1210 XHG=134 XHG=186 XHG=314 XHG=465 XHG=588 XHG=673 XHG=844 XHG=988 XHG=1048 XHG=1178 XHG=1228 YBD=2145 YBD=2147 YBD=2154 YBD=2144 YBD=2143 YBD=2151 YBD=2145 YBD=2143 YHG=2119>to YHG=2116>‘‘ YHG=2117>Repeat YHG=2124>every YHG=2116>few YHG=2115>minutes YHG=2115>during YHG=2116>20 YHG=2114>min. YHG=2115>A YHG=2114>sub1.&dquo; XBD=1433 XBD=1529 XBD=1572 XBD=1678 XBD=1816 XBD=1958 XBD=2038 XBD=2134 XBD=2296 XBD=2387 XBD=2482 XBD=2575 XHG=1370 XHG=1454 XHG=1551 XHG=1592 XHG=1698 XHG=1835 XHG=1977 XHG=2057 XHG=2153 XHG=2316 XHG=2407 XHG=2503 YBD=2164 YBD=2171 YBD=2163 YBD=2162 YHG=2136>out YHG=2143>a YHG=2134>tube YHG=2134>before YHG=2135>taking YHG=2136>the YHG=2135>first YHG=2135>reading YHG=2135>and YHG=2134>into YHG=2134>this " XBD=265 XBD=348 XBD=486 XBD=566 XBD=619 XBD=815 XBD=999 XBD=1078 XBD=1256 XBD=1314 XHG=135 XHG=280 XHG=363 XHG=503 XHG=582 XHG=634 XHG=831 XHG=1016 XHG=1093 XHG=1274 YBD=2185 YBD=2192 YBD=2184 YBD=2183 YBD=2191 YBD=2182 YHG=2156>stantial YHG=2156>and YHG=2155>steady YHG=2155>fall YHG=2155>in YHG=2155>flow-time YHG=2154>indicates YHG=2154>the YHG=2162>presence YHG=2153>of " XBD=1457 XBD=1537 XBD=1606 XBD=1667 XBD=1743 XBD=1896 XBD=1998 XBD=2059 XBD=2261 XBD=2317 XBD=2464 XHG=1370 XHG=1475 XHG=1555 XHG=1627 XHG=1682 XHG=1760 XHG=1914 XHG=2016 XHG=2078 XHG=2280 XHG=2336 YBD=2201 YBD=2202 YBD=2212 YBD=2211 YBD=2203 YHG=2173>fluid YHG=2173>the YHG=2174>tip YHG=2173>of YHG=2174>the YHG=2175>pipette YHG=2184>may YHG=2175>be YHG=2175>immersed YHG=2183>as YHG=2173>before. " XBD=392 XHG=135 YBD=2232 YHG=2196>hyaluronidase.

&dquo;

11

"" XBD=1311 XBD=669 XBD=817 XBD=958 XBD=1014 XBD=1180 ’Important XHG=596 XHG=682 XHG=831 XHG=971 XHG=1027 XHG=1192 poi.nt8.-((t) YBD=2262 YBD=2270 YBD=2261 YBD=2260 YBD=2261 YBD=2260 YHG=2234>The YHG=2234>pipette YHG=2225>should YHG=2233>be YHG=2233>warmed YHG=2232>before XBD=1764 XBD=1944 XBD=2040 XBD=2134 XBD=2376 XBD=2445 XHG=1498 XHG=1778 XHG=1961 XHG=2054 XHG=2150 XHG=2394 YBD=2282 YBD=2281 YHG=2257>PROVISIONAL YHG=2259>METHOD YHG=2259>FOR YHG=2259>THE YHG=2244>DETECTION YHG=2258>OF " " " " " " " " " " " " " " " " " " " " " " " " "

XBD=1310 XBD=209 XBD=358 XBD=420 XBD=581 XBD=657 XBD=760 XBD=821 XBD=910 XBD=1028 XBD=1129 XBD=1184 XHG=136 XHG=223 XHG=373 XHG=436 XHG=596 XHG=673 XHG=774 XHG=925 XHG=1044 XHG=1145 XHG=1198 YBD=2303 YBD=2310 YBD=2308 YBD=2301 YBD=2309 YBD=2300 YBD=2300 YBD=2299 YBD=2301 YBD=2300 YHG=2275>each YHG=2275>reading YHG=2275>by YHG=2274>sucking YHG=2273>the YHG=2273>fluid YHG=2282>up YHG=2273>and YHG=2272>down YHG=2281>once YHG=2281>or YHG=2271>twice. XBD=1876XHG=839 XBD=1937 XBD=2102 XBD=2331 XHG=1612 XHG=1892 XHG=1952 XHG=2113 YBD=2321 YBD=2320 YHG=2297>LECITHINASE YHG=2298>IN YHG=2299>WOUND YHG=2285>EXUDATES XBD=175 XHG=811 XBD=332 XBD=381 XBD=453 XBD=601 XBD=741 XBD=798 XBD=960 XBD=1011 XBD=1122 XBD=1219 XBD=1311 XHG=136 XHG=188 XHG=345 XHG=394 XHG=466 XHG=615 XHG=754 XHG=974YBD=2340 XHG=1025 XHG=1135 XHG=1232 YBD=2349 YBD=2342 YBD=2341 YBD=2350 YBD=2339 YBD=2339 YBD=2347 YHG=2315>(b) YHG=2314>Bubbles YHG=2314>in YHG=2314>the YHG=2313>pipette YHG=2311>should YHG=2312>be YHG=2311>avoided YHG=2320>as YHG=2311>these YHG=2320>may YHG=2311>give XBD=1515 XBD=1701 XBD=1796 XBD=1919 XBD=2125 XBD=2345 XBD=2416 XBD=2573 XHG=1423 XHG=1540 XHG=1725 XHG=1822 XHG=1946 XHG=2150 XHG=2372 XHG=2445 YBD=2379 YBD=2376 YBD=2383 YBD=2374 YBD=2375 YHG=2345>This YHG=2345>method YHG=2339>has YHG=2345>been YHG=2345>designed YHG=2344>primarily YHG=2344>to YHG=2344>detect XBD=208 XHG=777 XBD=384 XBD=475 XBD=565 XBD=632 XBD=690 XBD=763 XBD=913 XBD=1054 XBD=1112 XBD=1312 XHG=134 XHG=222 XHG=433 XHG=492 XHG=581 XHG=650 XHG=701 XHG=928YBD=2388 XHG=1068 XHG=1127 YBD=2381 YBD=2389 YBD=2380 YBD=2379 YBD=2379 YBD=2378 YHG=2354>false YHG=2354>readings. YHG=2354>(c) YHG=2352>The YHG=2352>tip YHG=2351>of YHG=2351>the YHG=2351>pipette YHG=2350>should YHG=2351>be YHG=2351>immersed XBD=231 XBD=309 XBD=462 XBD=523 XBD=601 XBD=709 XBD=767 XBD=848 XBD=954 XBD=1014 XBD=1142 XBD=1310 XHG=136 XHG=250 XHG=329 XHG=483 XHG=540 XHG=621 XHG=729 XHG=787 XHG=976 XHG=1035 XHG=1162 YBD=2422 YBD=2421 YBD=2418 YBD=2418 YBD=2419 YHG=2392>below YHG=2393>the YHG=2391>surface YHG=2390>of YHG=2392>the YHG=2390>fluid YHG=2391>in YHG=2390>the YHG=2393>to YHG=2390>avoid YHG=2390>surfaceXBD=1430XHG=870 XBD=1591 XBD=1780 XBD=2064 XBD=2125 XBD=2169 XBD=2341 XBD=2417 XBD=2572 XHG=1376YBD=2419 XHG=1453 XHG=1612 XHG=1802 XHG=2084 XHG=2146 XHG=2191 XHG=2364 XHG=2439 YBD=2416YHG=2391>tube YBD=2415 YBD=2423 YBD=2424 YBD=2420 YBD=2421 YBD=2414 YHG=2384>Cl. YHG=2385>welchii YHG=2385>actively YHG=2385>proliferating YHG=2385>in YHG=2394>a YHG=2375>wound, YHG=2384>by YHG=2393>means XBD=1414 XBD=1524 XBD=1791 XBD=1859 XBD=2088 XBD=2249 XBD=2505 XBD=2575 XHG=1374 XHG=1456 XHG=1568 XHG=1825 XHG=1893 XHG=2158 XHG=2282 XHG=2539 YBD=2455 YBD=2462 YBD=2453 YBD=2454 YHG=2423>of YHG=2424>the YHG=2424>lecithinase YHG=2424>it YHG=2425>produces. YHG=2424>This YHG=2423>lecithinase YHG=2423>is XBD=257 XHG=785 XBD=395 XBD=491 XBD=580 XBD=645 XBD=701 XBD=772 XBD=920 XBD=1059 XBD=1114 XBD=1313 XHG=135 XHG=269 XHG=443 XHG=506 XHG=593 XHG=661 XHG=711 XHG=933YBD=2467 XHG=1071 XHG=1128 YBD=2465 YBD=2460 YBD=2466 YBD=2457 YBD=2458 YBD=2458 YBD=2458 YHG=2432>tension YHG=2432>effects. YHG=2431>(d) YHG=2431>The YHG=2430>tip YHG=2429>of YHG=2430>the YHG=2430>pipette YHG=2429>should YHG=2429>be YHG=2430>immersed XBD=1555XHG=887 XBD=1672 XBD=1760 XBD=1906 XBD=2010 XBD=2263 XBD=2365 XBD=2573 XHG=1373 XHG=1575 XHG=1693 XHG=1783 XHG=1955 XHG=2035 XHG=2283 XHG=2387 YBD=2494 YBD=2493 YBD=2492 YBD=2500 YHG=2463>identical YHG=2464>with YHG=2464>the YHG=2464>toxin. YHG=2463>Cl. YHG=2462>mdematiens YHG=2462>also YHG=2463>produces XBD=167 XBD=280 XBD=467 XBD=605 XBD=671 XBD=744 XBD=859 XBD=924 XBD=1071 XBD=1120 XBD=1312 XHG=136 XHG=192 XHG=306 XHG=495 XHG=633 XHG=698 XHG=771 XHG=953 XHG=1098 XHG=1147 YBD=2499 YBD=2506 YBD=2498 YBD=2497 YBD=2498 YBD=2497 YBD=2498 YHG=2473>to YHG=2480>some YHG=2473>constant YHG=2470>depth YHG=2469>in YHG=2469>all YHG=2472>tests YHG=2472>to YHG=2478>secure YHG=2479>a YHG=2472>constant XBD=1484XHG=867 XBD=1710 XBD=1765 XBD=1803 XBD=2039 XBD=2398 XBD=2574 XHG=1373 XHG=1500 XHG=1726 XHG=1779 XHG=1817 XHG=2053 XHG=2415 YBD=2540 YBD=2533 YBD=2539 YBD=2532 YHG=2502>small YHG=2503>quantities YHG=2503>of YHG=2512>a YHG=2502>lecithinase YHG=2502>immunologically YHG=2501>distinct XBD=275 XBD=390 XBD=519 XBD=566 XBD=664 XBD=849 XBD=915 XBD=1131 XBD=1233 XBD=1312 XHG=135 XHG=292 XHG=436 XHG=538 XHG=585 XHG=682 XHG=932 XHG=1150 XHG=1251 YBD=2545 YBD=2539 YBD=2536 YBD=2537 YBD=2544 YBD=2544 YBD=2544 YBD=2537 YHG=2519>pressure YHG=2510>head. YHG=2509>This YHG=2510>is YHG=2509>best YHG=2508>achieved YHG=2509>by YHG=2508>immersing YHG=2509>only YHG=2508>the XBD=1472 XBD=1580 XBD=1642 XBD=1719 XBD=1979 XBD=2030 XBD=2219 XBD=2328 XBD=2573 XHG=1372 XHG=1490 XHG=1602 XHG=1664 XHG=1940 XHG=1998 XHG=2049 XHG=2238 XHG=2347 YBD=2572 YBD=2578 YBD=2570 YBD=2571 YHG=2541>from YHG=2541>that YHG=2542>of YHG=2541>Cl. YHG=2541>It YHG=2541>is YHG=2541>unlikely YHG=2540>that YHG=2541>lecithinase XBD=200 XHG=935 XBD=238 XBD=293 XBD=331 XBD=432 XBD=523 XBD=617 XBD=772 XBD=916 XBD=979 XBD=1079 XBD=1239 XBD=1314 XHG=135 XHG=219 XHG=256 XHG=313 XHG=349 XHG=481 XHG=545 XHG=635 XHG=792 XHG=997YBD=2576 XHG=1097 XHG=1258 YBD=2577 YBD=2575 YBD=2584 YBD=2576 YBD=2584 YBD=2576 YHG=2549>first YHG=2551>4 YHG=2558>or YHG=2551>5 YHG=2557>mm. YHG=2549>(e) YHG=2548>The YHG=2548>pipette YHG=2547>should YHG=2548>be YHG=2548>held YHG=2547>upright YHG=2547>for XBD=187 XBD=294 XBD=442 XHG=136 XHG=206 YBD=2615 YBD=2616 YHG=2588>the YHG=2597>same YHG=2596>reason. XBD=1495XHG=312 XBD=1553 XBD=1709 XBD=1911 XBD=2006 XBD=2209 XBD=2326 XBD=2401 XHG=1372 XHG=1510 XHG=1568 XHG=1726 XHG=1928 XHG=2023 XHG=2227 XHG=2346 YBD=2611 YBD=2617 YBD=2609 YBD=2610 YHG=2580>found YHG=2581>in YHG=2580>wound YHG=2580>exudates YHG=2579>will YHG=2580>originate YHG=2580>from YHG=2579>Cl. XBD=1480 XHG=2421 XBD=1608 XBD=1703 XBD=1912 XBD=2128 XBD=2215 XBD=2394 XBD=2573 XHG=1374 YBD=2650 XHG=1507 XHG=1635 XHG=1730 XHG=1937 XHG=2154 XHG=2243 YBD=2656 YHG=2619>smaller YBD=2651 YBD=2657 YHG=2620>tiens, YHG=2620>since YHG=2621>the YHG=2619>amounts YHG=2619>produced YHG=2629>are YHG=2619>usually XBD=255 XBD=440 XBD=529 XBD=695 XBD=844 XBD=1064 XBD=1220 XHG=213 XHG=270 XHG=457 XHG=546 XHG=861 XHG=1081 YBD=2694 YBD=2695 YBD=2695 YBD=2695 YHG=2675>TO YHG=2675>INDICATE YHG=2675>THE YHG=2674>NATURE YHG=2675>OF YHG=2675>BACTERIAL YHG=2675>SOURCE THE XBD=1471XHG=713 XBD=1568 XBD=1635 XBD=1835 XBD=1909 XBD=1997 XBD=2098 XBD=2325 XBD=2571 XHG=1371 XHG=1490 XHG=1588 XHG=1659 XHG=1856 XHG=1929 XHG=2018 XHG=2119 XHG=2371 YBD=2689 YBD=2696 YBD=2688 YBD=2690 YBD=2697 YHG=2659>than YHG=2660>will YHG=2659>be YHG=2658>detected YHG=2659>by YHG=2659>the YHG=2658>test YHG=2659>described. YHG=2659>Adequate XBD=572 XBD=900 XHG=527 YBD=2734 YBD=2735 YHG=2714>OF YHG=2714>HYALURONIDASE XBD=1554XHG=587 XBD=1755 XBD=1867 XBD=1979 XBD=2117 XBD=2172 XBD=2301 XBD=2392 XBD=2571 XHG=1375 XHG=1568 XHG=1769 XHG=1883 XHG=1991 XHG=2128 XHG=2185 XHG=2313 XHG=2405 YBD=2734 YBD=2727 YBD=2728 YBD=2729 YBD=2737 YBD=2736 YHG=2699>controls, YHG=2699>however, YHG=2698>have YHG=2698>been YHG=2698>added YHG=2699>to YHG=2699>avoid YHG=2708>any YHG=2698>possible XBD=1573 XBD=1762 XBD=1883 XBD=1978 XBD=2247 XHG=1375 YBD=2768 XHG=1589 XHG=1776 XHG=1900 XHG=1995 YBD=2766 YHG=2737>lecithinases. YBD=2767 YHG=2737>confusion YHG=2737>between YHG=2736>these YHG=2737>two 0 XBD=291 XBD=347 XBD=484 XBD=651 XBD=696 XBD=872 XBD=960 XBD=1061 XBD=1117 XBD=1150 XBD=1312 XHG=207 XHG=308 XHG=356 XHG=499 XHG=667 XHG=710 XHG=887 XHG=977YBD=2787 XHG=1077 XHG=1128 XHG=1164 YBD=2788 YBD=2786 YBD=2795 YBD=2787 YBD=2786 YBD=2787 YHG=2759>1 YHG=2757>of YHG=2758>wound YHG=2759>exudate YHG=2759>is YHG=2758>pipetted YHG=2760>into YHG=2759>each YHG=2758>of YHG=2768>a YHG=2759>number ml. ml. XBD=282 XBD=375 XBD=476 XBD=640 XBD=685 XBD=815 XBD=884 XBD=1075 XBD=1313 XHG=136 XHG=327 XHG=391 XHG=491 XHG=656 XHG=701 XHG=831 XHG=977 XHG=1089 YBD=2825 YBD=2826 YBD=2826 YBD=2835 YHG=2796>of YHG=2798>To YHG=2797>each YHG=2798>of YHG=2798>is YHG=2798>added YHG=2798>0-1 YHG=2798>of YHG=2798>appropriate tubes. these an XBD=2177 XHG=1988 YBD=2852 YHG=2828>REQUIRED * XBD=1312XHG=860 XBD=338 XBD=499 XBD=553 XBD=643 XBD=731 XBD=844 XBD=897 XBD=1123 XBD=1193 XBD=1230 XHG=136 XHG=386 XHG=515 XHG=569 XHG=660 XHG=746 XHG=911YBD=2864 XHG=1137 XHG=1208 XHG=1244 YBD=2863 YBD=2873 YBD=2865 YBD=2865 YBD=2866 YHG=2837>antiserum.* YHG=2836>Shake YHG=2839>to YHG=2837>mix YHG=2837>and YHG=2837>place YHG=2837>in YHG=2837>water-bath YHG=2836>for YHG=2846>a YHG=2836>few XBD=270 XBD=323 XBD=456 XBD=616 XBD=742 XBD=831 XBD=885 XBD=963 XBD=1056 XBD=1159 XBD=1233 XBD=1309 XHG=134 XHG=286 XHG=339 XHG=503 XHG=632 XHG=760 XHG=846 XHG=980YBD=2903 XHG=1074 XHG=1178 XHG=1250 YBD=2902 YBD=2904 YBD=2903 YBD=2903 YBD=2905 YHG=2875>minutes YHG=2884>warm. YHG=2875>When YHG=2885>warm YHG=2875>add YHG=2877>to YHG=2875>the YHG=2876>first YHG=2875>tube ; YHG=2877>0-8 YHG=2876>ml. XBD=1446XHG=903 XBD=2113 XBD=2271 XBD=2452 XBD=2574 XHG=1420 XHG=2081 XHG=2139 XHG=2418 XHG=2479 YBD=2908 YBD=2909 YBD=2910 YBD=2901 YHG=2874>1. YHG=2882>g. YHG=2874>borax YHG=2883>g. YHG=2873>boric XBD=1474 XBD=1606 XBD=1669 XBD=1839 XBD=2031 XBD=2180 XBD=2248 XBD=2367 XBD=2457 XBD=2576 XHG=1373 XHG=1507 XHG=1638 XHG=1703 XHG=1869 XHG=2077 XHG=2210 XHG=2282 XHG=2396 XHG=2479 YBD=2944 YBD=2947 YBD=2939 YBD=2942 YBD=2941 YBD=2940 YHG=2911>acid; YHG=2911>0-487 YHG=2919>g. YHG=2910>sodium YHG=2911>chloride. YHG=2912>Make YHG=2915>to YHG=2914>1000 YHG=2913>ml. YHG=2911>’with XBD=169 XHG=875 XBD=286 XBD=462 XBD=662 XBD=758 XBD=852 XBD=962 XBD=1007 XBD=1098 XBD=1211 XBD=1309 XHG=135 XHG=185 XHG=307 XHG=485 XHG=686 XHG=779 XHG=984YBD=2943 XHG=1029 XHG=1121 XHG=1235 YBD=2939 YBD=2941 YBD=2942 YBD=2943 YBD=2950 YHG=2912>of YHG=2922>warm YHG=2914>buffered YHG=2915>substrate YHG=2914>and YHG=2915>mix YHG=2914>with YHG=2924>a YHG=2915>fine YHG=2914>glass YHG=2915>rod. substrate to XBD=782 XBD=926 XBD=1030 XBD=1119 XBD=1219 XBD=1312 XBD=1483 XBD=1649 XBD=1829 XBD=1958 XBD=2089 XBD=2218 XBD=2280 XBD=2466 XBD=2575 XHG=718 XHG=943YBD=2982 XHG=1047 XHG=1135 XHG=1240 XHG=1446 XHG=1501 XHG=1667 XHG=1876 XHG=1976 XHG=2106 XHG=2236 XHG=2297 XHG=2483 YBD=2981 YBD=2981 YBD=2981 YBD=2987 YBD=2982 YBD=2979 YBD=2988 YBD=2980 YBD=2978 YHG=2953>the YHG=2947>second YHG=2953>tube YHG=2953>and YHG=2953>mix, YHG=2954>and YHG=2951>M YHG=2950>calcium YHG=2949>chloride. YHG=2950>This YHG=2951>buffer YHG=2952>might YHG=2953>be YHG=2951>obtained YHG=2949>from XBD=224 XHG=797 XBD=260 XBD=357 XBD=448 XHG=126 XHG=242 XHG=276 XHG=377 YBD=2988 YBD=2980 YHG=2950>After YHG=2953>5 YHG=2952>min. YHG=2952>add XBD=1395 XBD=1578 XBD=1684 XHG=1373 XHG=1411 XHG=1592 YBD=3018 YHG=2998>a YHG=2989>chemical YHG=2989>firm. XBD=167 XHG=849 XBD=231 XBD=326 XBD=424 XBD=609 XBD=800 XBD=835 XBD=933 XBD=1040 XBD=1114 XBD=1312 XHG=135 XHG=180 XHG=245 XHG=339 XHG=437 XHG=624 XHG=817 XHG=949YBD=3021 XHG=1053 XHG=1127 YBD=3017 YBD=3025 YBD=3019 YBD=3028 YBD=3021 YBD=3020 YBD=3028 YHG=2998>so YHG=3000>on, YHG=2991>each YHG=2991>tube YHG=2993>receiving YHG=2993>substrate YHG=2994>5 YHG=2991>min. YHG=2992>after YHG=2992>the YHG=2992>preceding

welchii. cdema-

MATERIALS

Borate-calcizem 0-1

" YBD=3058 YHG=3029>tube. " XBD=219 XBD=1451XHG=135 XBD=2083 XBD=2265 XHG=1420 XHG=1946 XHG=2104 YBD=3056 YBD=3058 YHG=3030>2. YHG=3029>sodium YHG=3030>chloride.

" " " " " " " " " " " " "

’

XBD=2580 XBD=1450 XBD=1636 XBD=1910 XBD=1959 XBD=2095 XBD=2153 XBD=2318 XBD=2520 XHG=1421 XHG=1471 XHG=1655 XHG=1931 XHG=1979 XHG=2115 XHG=2173 XHG=2338 XHG=2539 YBD=3103 YBD=3097 YBD=3105 YBD=3104 YBD=3095 YHG=3069>3. YHG=3067>Egg-yolk YHG=3068>substrate.-It YHG=3069>is YHG=3068>hoped YHG=3072>to YHG=3069>provide YHG=3068>ampoules YHG=3066>of XBD=212 XHG=857 XBD=311 XBD=424 XBD=575 XBD=775 XBD=834 XBD=919 XBD=1016 XBD=1124 XBD=1229 XBD=1312 XHG=175 XHG=231 XHG=334 XHG=445 XHG=597 XHG=797 XHG=939YBD=3098 XHG=1038 XHG=1146 XHG=1251 YBD=3096 YBD=3097 YBD=3106 YBD=3099 YBD=3099 YBD=3099 YBD=3098 YHG=3069>30 YHG=3068>min. YHG=3068>after YHG=3070>adding YHG=3071>substrate YHG=3073>to YHG=3070>the YHG=3071>first YHG=3070>tube YHG=3070>take YHG=3071>the XBD=1473XHG=829 XBD=1688 XBD=1937 XBD=2136 XBD=2257 XBD=2355 XBD=2575 XHG=1375 XHG=1510 XHG=1726 XHG=1975 XHG=2171 XHG=2293 XHG=2391 YBD=3133 YBD=3142 YBD=3143 YBD=3136 YBD=3134 YBD=3141 YHG=3105>dried YHG=3106>substrate YHG=3106>eventually, YHG=3108>together YHG=3107>with YHG=3106>the YHG=3114>necessary XBD=296 XBD=450 XBD=507 XBD=581 XBD=760 XBD=816 XBD=900 XBD=1014 XBD=1210 XBD=1313 XHG=135 XHG=310 XHG=467 XHG=519 XHG=597 XHG=777 XHG=916YBD=3137 XHG=1031 XHG=1227 YBD=3134 YBD=3144 YBD=3136 YBD=3143 YBD=3146YHG=3109>this YBD=3137 YHG=3105>flow-time YHG=3107>reading YHG=3109>the YHG=3111>contents YHG=3109>of YHG=3109>tube, YHG=3109>repeating YHG=3118>once twice and XBD=1573 XBD=1834 XBD=1918 XBD=2112 XBD=2161 XBD=2204 XBD=2437 XBD=2498 XBD=2577 XHG=1376 XHG=1619 XHG=1857 XHG=1938 XHG=2133 XHG=2184 XHG=2227 XHG=2457 XHG=2515 YBD=3172 YBD=3179 YBD=3174 YBD=3181 YBD=3173 YBD=3180 YBD=3171 YHG=3144>directions. YHG=3144>Meanwhile, YHG=3146>the YHG=3146>following YHG=3146>is YHG=3155>a YHG=3144>description YHG=3143>of YHG=3143>the XBD=168 XBD=505 XBD=587 XBD=722 XBD=877 XBD=967 XBD=1052 XBD=1207 XBD=1314 XHG=136 XHG=384 XHG=526 XHG=607 XHG=768 XHG=896 XHG=987 XHG=1072 XHG=1226 YBD=3171 YBD=3182 YBD=3177 YBD=3176 YBD=3183 YBD=3184 YBD=3176 YHG=3152>or YHG=3147>taking YHG=3149>the YHG=3157>mean. YHG=3148>Wash YHG=3148>and YHG=3148>dry YHG=3147>pipette YHG=3147>with XBD=1521 XBD=1597 XBD=1762 XBD=1813 XBD=1926 XBD=2051 XBD=2167 XBD=2297 XBD=2386 XBD=2477 XBD=2575 XHG=1374 XHG=1542 XHG=1618 XHG=1784 XHG=1834 XHG=1947 XHG=2063 XHG=2202 XHG=2320 XHG=2407 XHG=2500 YBD=3212 YBD=3219 YBD=3211 YBD=3210 YBD=3218 YHG=3182>method YHG=3182>for YHG=3184>making YHG=3184>it YHG=3183>from YHG=3184>fresh YHG=3193>eggs YHG=3184>Take YHG=3192>one YHG=3182>hen YHG=3191>egg, : XBD=228 XHG=873 XBD=397 XBD=462 XBD=624 XBD=714 XBD=752 XBD=854 XBD=961 XBD=1061 XBD=1101 XBD=1260 XBD=1317 XHG=135 XHG=243 XHG=414 XHG=479 XHG=643 XHG=733 XHG=770 XHG=978YBD=3214 XHG=1079 XHG=1117 XHG=1277 YBD=3211 YBD=3212 YBD=3221 YBD=3215 YBD=3214 YBD=3222YHG=3186>later YBD=3214 YHG=3186>water YHG=3182>followed YHG=3185>by YHG=3189>acetone YHG=3187>and YHG=3188>5 YHG=3186>min. YHG=3186>take YHG=3195>a YHG=3186>reading YHG=3186>of XBD=1536XHG=893 XBD=1620 XBD=1728 XBD=1839 XBD=1923 XBD=2060 XBD=2156 XBD=2273 XBD=2326 XBD=2433 XBD=2575 XBD=186 XBD=323 XBD=528 XBD=585 XBD=663 XBD=770 XBD=874 XBD=978 XBD=1018 XBD=1122 XBD=1234 XBD=1314 XHG=135 XHG=204 XHG=341 XHG=548 XHG=605 XHG=683 XHG=789 XHG=1375 XHG=1558 XHG=1642 XHG=1748 XHG=1861 XHG=1943 XHG=2086 XHG=2177 XHG=2295 XHG=2345 XHG=2456 XHG=999 XHG=1037 XHG=1143 XHG=1253 YBD=3249 YBD=3250 YBD=3258 YBD=3252 YBD=3256 YBD=3254 YBD=3253 YBD=3253 YBD=3249 YBD=3258 YBD=3251 YBD=3256 YBD=3248 YBD=3250 YBD=3254 YBD=3252 YHG=3221>the YHG=3223>second YHG=3215>tube, YHG=3234>so YHG=3235>on, YHG=3225>with YHG=3225>each YHG=3225>tube YHG=3226>5 YHG=3224>separate YHG=3221>the YHG=3222>yolk YHG=3222>from YHG=3222>white, YHG=3222>and YHG=3221>wash YHG=3221>it YHG=3220>with YHG=3221>saline. YHG=3224>min. YHG=3225>after YHG=3224>the and XBD=1475XHG=772 XBD=1571 XBD=1624 XBD=1860 XBD=1969 XBD=2058 XBD=2141 XBD=2207 XBD=2345 XBD=2443 XBD=2577 XHG=1376 XHG=1497 XHG=1595 XHG=1648 XHG=1882 XHG=1995 XHG=2082 XHG=2168 XHG=2228 XHG=2371 XHG=2466 YBD=3298 YBD=3290 YBD=3289 YBD=3293 YBD=3287 YBD=3288 YHG=3260>Then YHG=3260>mix YHG=3260>it YHG=3261>thoroughly YHG=3262>with YHG=3262>250 YHG=3260>ml. YHG=3260>of YHG=3260>saline, YHG=3260>add YHG=3260>about XBD=300 XBD=394 XBD=535 XBD=643 XBD=750 XBD=800 XBD=1011 XBD=1117 XBD=1314 XHG=135 XHG=320 XHG=440 XHG=557 XHG=664 XHG=820 XHG=1030 XHG=1137 YBD=3298 YBD=3291 YBD=3292 YBD=3292 YBD=3292 YHG=3261>preceding YHG=3271>one. YHG=3263>Thus YHG=3264>each YHG=3264>tube YHG=3264>is YHG=3264>incubated YHG=3264>with YHG=3263>substrate

30, XHG=498 XBD=2576 XBD=1419 XBD=1468 XBD=1529 XBD=1738 XBD=1826 XBD=1933 XBD=2101 XBD=2139 XBD=2332 XBD=2439 XHG=1379 XHG=1436 XHG=1489 XHG=1541 XHG=1754 XHG=1841 XHG=1948 XHG=2118 XHG=2155 XHG=2347 XHG=2455 YBD=3333 YBD=3328 YBD=3334 YBD=3327 YBD=3326 YBD=3335 YHG=3300>10 YHG=3307>g. YHG=3298>of YHG=3300>kieselguhr YHG=3300>and YHG=3300>filter YHG=3299>through YHG=3308>a YHG=3298>hardened YHG=3298>filter YHG=3308>paper. XBD=181 XBD=338 XBD=478 XBD=653 XHG=133 XHG=259 XHG=361 YBD=3326 YBD=3330 YBD=3329 YBD=3340 YHG=3298>for YHG=3300>min. YHG=3301>before YHG=3303>reading. XBD=1457 XHG=2286 XBD=1693 XBD=1745 XBD=1821 XBD=1965 XBD=2038 XBD=2175 XBD=2273 XBD=2576 XHG=1375 YBD=3373 XHG=1470 XHG=1707 XHG=1760 XHG=1834 XHG=1981 XHG=2057 XHG=2188 YBD=3364 YHG=3338>concentration). YBD=3366 YBD=3372 YBD=3370 YBD=3363 YHG=3337>Add: YHG=3335>merthiolate YHG=3341>to YHG=3339>the YHG=3339>filtrate YHG=3339>(1 YHG=3337>10,000 YHG=3337>final XBD=787 XBD=890 XBD=990 XBD=1109 XBD=1166 XBD=1317 Antisera.-Commercial XHG=610 XHG=807 XHG=910 XHG=1009 XHG=1130 XHG=1187 YBD=3401 YBD=3400 YBD=3400 YBD=3399 YHG=3375>antitoxins YHG=3375>have YHG=3376>been YHG=3377>found YHG=3376>to YHG=3375>contain XBD=1459 XBD=1547 XBD=1650 XBD=1726 XBD=1831 XBD=1936 XBD=1994 XBD=2231 XBD=2494 XHG=1376 XHG=1476 XHG=1564 XHG=1671 XHG=1745 XHG=1850 XHG=1955 XHG=2012 XHG=2249 YBD=3403 YBD=3412 YBD=3404 YBD=3405 YBD=3402 YBD=3410 YHG=3375>This YHG=3376>will YHG=3377>keep YHG=3377>for YHG=3378>7-14 YHG=3377>days YHG=3378>at YHG=3375>refrigerator YHG=3377>temperature. XBD=254 XBD=442 XBD=496 XBD=569 XBD=715 XBD=1070 XBD=1147 XBD=1192 XBD=1316 XHG=136 XHG=273 XHG=461 XHG=513 XHG=733 XHG=1113 XHG=1164 XHG=1209 YBD=3434 YBD=3430 YBD=3433 YBD=3433 YBD=3429 YBD=3432 YHG=3405>varying YHG=3404>quantities YHG=3407>of YHG=3405>the YHG=3405>specific YHG=3404>anti-hyaluronidase. YHG=3404>It YHG=3404>is YHG=3404>hoped XBD=1452XHG=589 XBD=1620 XBD=1824 XBD=2120 XBD=2307 XBD=2459 XBD=2577 XHG=1421 XHG=1481 XHG=1642 XHG=1853 XHG=2144 XHG=2330 XHG=2484 YBD=3444 YBD=3449 YBD=3448 YBD=3443 YHG=3416>4. YHG=3415>Welchii YHG=3416>antiserum YHG=3414>(W.A.).-Any YHG=3413>ordinary YHG=3415>2relchii YHG=3414>antiXBD=165 XBD=258 XBD=361 XBD=451 XBD=521 XBD=718 XBD=794 XBD=892 XBD=1101 XBD=1157 XBD=1212 XBD=1318 XHG=135 XHG=181 XHG=276 XHG=381 XHG=469 XHG=540 XHG=736 XHG=812 XHG=912 XHG=1121 XHG=1176 XHG=1232 YBD=3460 YBD=3463 YBD=3458 YBD=3459 YBD=3462 YBD=3458 YBD=3457 YHG=3432>to YHG=3434>issue YHG=3434>these YHG=3441>sera YHG=3436>for YHG=3434>diagnostic YHG=3440>use YHG=3433>with YHG=3434>particulars YHG=3439>as YHG=3434>to YHG=3432>their *

" XBD=1476 XBD=1536 XBD=1662 XBD=1838 XBD=1952 XBD=2033 XBD=2112 XBD=2268 XBD=2331 XHG=1374 XHG=1496 XHG=1553 XHG=1686 XHG=1858 XHG=1972 XHG=2053 XHG=2134 XHG=2292 XHG=2348 YBD=3481 YBD=3487 YBD=3479 YBD=3480 YBD=3482 YHG=3454>toxin YHG=3453>of YHG=3454>about YHG=3455>300-500 YHG=3453>units YHG=3460>per YHG=3452>ml. YHG=3452>diluted YHG=3453>1 YHG=3454>40. : " XBD=2401 XBD=264 XHG=136 YBD=3493 YHG=3463>potency.

buffer.-03184

Normal

salin.e.-0·BWo

2.061

360 antiserum XBD=183 XBD=439 XBD=1074 XBD=1306 XHG=152 XHG=925YBD=226 XHG=1092 YBD=225 YBD=225 YBD=225 YHG=196>5. YHG=195>lEdematiens (O.A.).-Any YHG=196>suitable YHG=196>codematiens " XBD=2463 XBD=2537XHG=210 XBD=1566 XBD=1623 XBD=1771 XBD=1990 XBD=2040 XBD=2260 XHG=1369 XHG=1585 XHG=1641 XHG=1786 XHG=2006 XHG=2059 XHG=2278 XHG=2477 YBD=227 YBD=234 YBD=228 YHG=196>character YHG=197>of YHG=196>mixed YHG=196>infections YHG=198>is YHG=196>obviously YHG=206>necessary YHG=198>and 40. " XBD=1757 XBD=1440 XBD=1560 XHG=1369 XHG=1463 XHG=1579 YBD=266 YBD=273 YHG=236>has YHG=235>been YHG=236>planned. XBD=220 XHG=748 XBD=286 XBD=419 XBD=601 XBD=724 XBD=811 XBD=895 XBD=1059 XBD=1129 XHG=835 XHG=103 XHG=245 XHG=307 XHG=447 XHG=626 XHG=923YBD=264 XHG=1088 YBD=263 YBD=266 YBD=265 YBD=273 YBD=263 YBD=263 YHG=243>serum YHG=235>of YHG=236>about YHG=238>300-500 YHG=237>units YHG=244>per YHG=235>ml. YHG=235>diluted YHG=236>1 : XBD=1308 XHG=1244 YBD=264 YHG=235>We XBD=1496 XBD=1590 XBD=1772 XBD=1855 XBD=2010 XBD=2192 XBD=2309 XBD=2361 XHG=1419YBD=305 XHG=1523 XHG=1621 XHG=1798 XHG=1884 XHG=2038 XHG=2219 XHG=2338 XHG=2388 YBD=304YHG=285>serum. YBD=312 YBD=305 YBD=311 YBD=303 YBD=310 YHG=274>For YHG=274>the YHG=275>present YHG=284>we YHG=275>would YHG=275>suggest YHG=273>that YHG=283>a YHG=266>negative " XBD=2536 XBD=197 XHG=524 XBD=258 XBD=409 XBD=502 XBD=651 XHG=104 XHG=220 XHG=280 XHG=430 YBD=312 YBD=303 YBD=313 YHG=274>hope YHG=278>to YHG=276>supply YHG=276>this

,

" XBD=2411 XBD=2537 XHG=2426 XBD=1491 XBD=1607 XBD=1691 XBD=1974 XBD=2031 XBD=2074 XBD=2232 XHG=1370 YBD=343 XHG=1508 XHG=1624 XHG=1710 XHG=1993 XHG=2050 XHG=2092 XHG=2249 YBD=344 YHG=312>should YBD=342 YHG=313>result YHG=313>from YHG=314>the YHG=313>examination YHG=313>of YHG=323>a YHG=313>wound YHG=312>exudate " XBD=2537 XBD=1443 XBD=1595 XBD=1638 XBD=1749 XBD=1875 XBD=1934 XBD=2117 XBD=2213 XBD=2365 XHG=1371 XHG=1459 XHG=1613 XHG=1656 XHG=1768 XHG=1894 XHG=1951 XHG=2134 XHG=2231 XHG=2382 YBD=383 YBD=382 YBD=389 YBD=381 YBD=388 YHG=352>not YHG=352>induce YHG=362>a YHG=353>false YHG=361>sense YHG=352>of YHG=351>security YHG=351>and YHG=350>should YHG=350>certainly XBD=451 XHG=562 XBD=546 XBD=882 XBD=944 XBD=1118 XHG=899 XHG=298 XHG=468 XHG=958YBD=394 YBD=388 YBD=387 YBD=386 YHG=367>METHOD YHG=366>AND YHG=363>INTERPRETATION YHG=364>OF YHG=361>RESTILTS

" XBD=2538 XBD=1443 XHG=2387 XBD=1516 XBD=1696 XBD=1759 XBD=2094 XBD=2187 XBD=2361 XHG=1372 YBD=419 XHG=1469 XHG=1538 XHG=1716 XHG=1782 XHG=2120 XHG=2212 YBD=423 YHG=389>adminisYBD=421 YBD=422 YBD=420 YHG=391>not YHG=392>be YHG=392>allowed YHG=391>to YHG=390>contra-indicate YHG=390>the YHG=390>routine

" XBD=1311 XBD=225 XHG=882 XBD=398 XBD=448 XBD=616 XBD=674 XBD=777 XBD=861 XBD=952 XBD=1138 XBD=1229 XHG=151 XHG=245 XHG=419 XHG=469 XHG=636 XHG=696 XHG=800 XHG=972YBD=444 XHG=1158 XHG=1249 YBD=442 YBD=441 YBD=439 YBD=445 YBD=438 YBD=445 YBD=439 YHG=412>The YHG=413>exudate YHG=413>is YHG=412>allowed YHG=413>to YHG=410>clot, YHG=409>the YHG=410>clot YHG=411>removed YHG=410>and YHG=410>the

"" XBD=2538 XBD=1521 XBD=1589 XBD=1820 XBD=1897 XBD=2231 XBD=2301 XBD=2437 XBD=2495 XHG=1371 XHG=1550 XHG=1622 XHG=1854 XHG=1931 XHG=2259 XHG=2334 XHG=2459 XHG=2521 YBD=462 YBD=461 YBD=468 YBD=464 YBD=457 YBD=459 YHG=431>tration YHG=431>of YHG=431>antitoxin YHG=440>or YHG=429>sulphonamide, YHG=438>or YHG=428>both, YHG=436>as YHG=438>a XBD=300 XBD=427 XBD=627 XBD=717 XBD=837 XBD=940 XBD=1017 XBD=1135 XHG=104 XHG=315 XHG=443 XHG=643 XHG=734 XHG=853 XHG=956 XHG=1034 XHG=1150 YBD=488 YBD=489 YBD=480 YBD=478 YBD=478 YBD=478 YBD=486 YHG=451>remaining YHG=452>liquid YHG=450>measured YHG=450>into YHG=449>tubes YHG=449>with YHG=449>the YHG=450>other YHG=452>reagents "" XBD=1312 XBD=1666 XBD=1885 XBD=1979 XBD=2195 XBD=2315 XBD=2443 XBD=2538 XHG=1373 XHG=1703 XHG=1944 XHG=2018 XHG=2236 XHG=2357 XHG=2476 YBD=508 YBD=500 YBD=499 YBD=506 YBD=504 YBD=498 YHG=471>precautionary YHG=479>measure. YHG=470>A YHG=469>negative YHG=467>test YHG=476>may YHG=467>also XBD=147 XHG=163 XBD=298 XHG=107 :YBD=519 YHG=491>follows YBD=520 YHG=499>as "" XBD=2539 XBD=1638 XBD=1704 XBD=1910 XBD=1976 XBD=2053 XBD=2238 XBD=2340 XBD=2400 XHG=1372 XHG=1659 XHG=1729 XHG=1935 XHG=2001 XHG=2079 XHG=2258 XHG=2362 XHG=2419 YBD=546 YBD=539 YBD=538 YBD=536 YBD=537 YHG=509>theoretically YHG=509>be YHG=509>obtained YHG=509>in YHG=517>an YHG=507>obvious YHG=516>case YHG=506>of YHG=505>clinical XBD=417 XHG=403 XBD=1046 XHG=1018 YBD=560 YBD=559 YHG=541>I YHG=539>n "" XBD=2539 XBD=1645 XBD=1715 XBD=1808 XBD=1942 XBD=2098 XBD=2238 XBD=2330 XHG=1374 XHG=1673 XHG=1741 XHG=1845 XHG=1972 XHG=2126 XHG=2264 XHG=2359 YBD=586 YBD=579 YBD=578 YBD=585 YBD=577 YBD=575 YBD=582 YHG=557>gas-gangrene YHG=548>in YHG=548>the YHG=548>later YHG=547>stages YHG=547>when YHG=546>the YHG=544>organisms XBD=159 XHG=883 XBD=229 XBD=279 XBD=395 XBD=455 XBD=797 XBD=866 XBD=916 XBD=1033 XBD=1092 XHG=107 XHG=174 XHG=245 XHG=293 XHG=411 XHG=748 XHG=813 XHG=929YBD=595 XHG=1049 YBD=597 YBD=600 YBD=596 YBD=601 YHG=573>0-5 YHG=572>ml. YHG=573>of YHG=572>buffer YHG=573>(1) YHG=571>0-5 YHG=570>ml. YHG=571>of YHG=571>buffer YHG=572>(1) "" XBD=2538 XBD=1438 XBD=1665 XBD=1725 XBD=1958 XBD=2031 XBD=2209 XBD=2346 XBD=2488 XHG=1374 XHG=1460 XHG=1681 XHG=1744 XHG=1997 XHG=2045 XHG=2229 XHG=2365 XHG=2504 YBD=618 YBD=625 YBD=617 YBD=624 YBD=614 YBD=615 YHG=596>are YHG=587>beginning YHG=587>to YHG=587>retrogress. YHG=587>A YHG=585>positive YHG=584>result YHG=583>should YHG=584>be 3-3 XBD=226 XBD=277 XBD=390 XBD=451 XBD=798 XBD=866 XBD=916 XBD=1028 XBD=1088 XHG=172 XHG=244 XHG=291 XHG=406 XHG=747 XHG=813 XHG=882 XHG=930YBD=625 XHG=1044 YBD=626 YBD=630 YBD=624 YBD=625 YBD=630 YHG=603>ml. YHG=603>of YHG=602>saline YHG=602>(2) YHG=600>2-3 YHG=600>ml. YHG=601>of YHG=600>saline YHG=601>(2) XBD=157 XHG=883 XBD=228 XBD=279 XBD=431 XBD=796 XBD=867 XBD=917 XBD=1070 XHG=107 XHG=174 XHG=245 XHG=291 XHG=747 XHG=814 XHG=930YBD=655 YBD=656 YBD=653 YBD=654 YBD=655 YHG=631>0-2 YHG=631>ml. YHG=632>of YHG=631>exudate YHG=630>0-2 YHG=630>ml. YHG=630>exudate " XBD=2539 XBD=1558 XBD=1610 XBD=1647 XBD=1828 XBD=1878 XBD=1954 XBD=2174 XBD=2225 XBD=2360 XHG=1372 XHG=1568 XHG=1623 XHG=1658 XHG=1840 XHG=1888 XHG=1966 XHG=2185 XHG=2236 XHG=2371 YBD=663YHG=631>of YBD=656 YBD=655 YBD=654 YBD=660 YHG=624>regarded YHG=635>as YHG=635>a YHG=625>warning YHG=626>of YHG=626>the YHG=624>likelihood YHG=624>of YHG=624>active YHG=623>infection, |>" " XBD=158 XBD=228 XBD=278 XBD=440 XBD=617 XBD=676 XBD=797 XBD=866 XBD=916 XBD=1077 XBD=1019 XHG=109 XHG=174 XHG=245 XHG=291 XHG=453 XHG=632 XHG=749 XHG=813 XHG=882 XHG=1033 XHG=930 YBD=685 YBD=689 YBD=687 YBD=683 YBD=684 YBD=690 YBD=684 YHG=661>1-0 YHG=661>ml. YHG=661>of YHG=660>egg-yolk YHG=658>substrate YHG=658>(3) YHG=660>1-0 YHG=659>ml. YHG=660>of YHG=661>(4) YHG=659>W.A. " XBD=1447 XBD=1545 XBD=1683 XBD=1737 XBD=1883 XBD=2049 XBD=2150 XBD=2227 XBD=2337 XBD=2463 XBD=2538 XHG=1374 XHG=1477 XHG=1577 XHG=1712 XHG=1768 XHG=1914 XHG=2080 XHG=2181 XHG=2258 XHG=2365 XHG=2487 YBD=702 YBD=695 YBD=693 YBD=700 YBD=694 YHG=665>but YHG=665>too YHG=664>great YHG=674>a YHG=665>stress YHG=665>should YHG=664>not YHG=664>be YHG=663>laid YHG=671>upon YHG=663>the " XBD=1318 XBD=796 XHG=883 XBD=865 XBD=916 XHG=749 XHG=929YBD=715 XHG=1269 YBD=713 YBD=718 YBD=719 YHG=689>1-0 YHG=688>ml. YHG=690>of YHG=688>egg-yolk YHG=685>(3) substrate " XBD=1253 XBD=2044 XBD=2099 XBD=2323 XBD=2430 XBD=2539 XHG=1953 XHG=2062 XHG=2114 XHG=2335 XHG=2441 YBD=741 YBD=733 YBD=740 YBD=732 YBD=734 YHG=703>type YHG=703>of YHG=702>organisms YHG=702>from YHG=702>which XBD=1603XHG=813 XBD=1809 XBD=1861 XHG=1374 XHG=1618 XHG=1823 YBD=737 YHG=703>differential YHG=703>diagnosis

the

I

XBD=430 XHG=389 XBD=1048 XHG=1012 YBD=771 YBD=771 YHG=752>in YHG=752>IV "" XBD=2006 XBD=1440 XBD=1616 XBD=1706 XBD=1823 XHG=1372 XHG=1461 XHG=1637 XHG=1726 XHG=1843 YBD=780 YBD=772 YBD=773 YHG=743>the YHG=752>enzyme YHG=743>has YHG=742>been YHG=743>derived. 0’5 0,5

XBD=228 XHG=882 XBD=278 XBD=396 XBD=456 XBD=866 XBD=916 XBD=1032 XBD=1091 XHG=174 XHG=245 XHG=292 XHG=410 XHG=812 XHG=929YBD=809 XHG=1048 YBD=808 YBD=812 YBD=807 YBD=808 YHG=783>ml. YHG=784>of YHG=783>buffer YHG=784>(1) YHG=784>ml. YHG=785>of YHG=783>buffer YHG=784>(1) " XBD=1584 XBD=1882 XBD=2011 XBD=2068 XBD=2268 XBD=2335 XBD=2539 XHG=1422 XHG=1610 XHG=1907 XHG=2034 XHG=2092 XHG=2292 XHG=2361 YBD=818 YBD=811 YBD=812 YHG=782>Further YHG=781>experimental YHG=782>work YHG=781>is YHG=781>required YHG=781>to YHG=780>determine " |>" XBD=159 XBD=228 XBD=278 XBD=390 XBD=451 XBD=796 XBD=865 XBD=916 XBD=1027 XBD=1091 XBD=1091 XHG=107 XHG=174 XHG=244 XHG=291 XHG=406 XHG=748 XHG=812 XHG=882 XHG=928 XHG=1042 XHG=1077 YBD=841 YBD=837 YBD=838 YBD=837 YBD=842 YBD=842 YHG=813>2’3 YHG=813>ml. YHG=813>of YHG=813>saline YHG=813>(2) YHG=814>3-5 YHG=812>ml. YHG=815>of YHG=813>saline YHG=812>(21 YHG=812>r " XBD=1438 XBD=1708 XBD=1784 XBD=1883 XBD=1994 XBD=2069 XBD=2392 XBD=2462 XBD=2539 XHG=1371 XHG=1473 XHG=1745 XHG=1816 XHG=1917 XHG=2030 XHG=2103 XHG=2424 XHG=2487 YBD=849 YBD=851 YBD=858 YBD=850 YBD=852 YHG=820>importance YHG=820>of YHG=821>the YHG=821>r6le YHG=821>of YHG=821>hyaluronidase YHG=820>in YHG=820>the " XBD=1312 XBD=157 XHG=812 XBD=228 XBD=278 XBD=431 XBD=795 XBD=915 XBD=1076 XBD=1251 XHG=107 XHG=174 XHG=245 XHG=291 XHG=748 XHG=927YBD=867 XHG=1088 XHG=1268 YBD=866 YBD=871 YBD=867 YBD=871 YHG=842>0 YHG=842>ml. YHG=842>of YHG=842>exudate YHG=842>1-0 YHG=842>ml.’of YHG=842>egg-yolk YHG=842>substrate YHG=843>(3) -2 " XBD=433 XBD=1552XHG=389 XBD=1727 XBD=1829 XBD=1938 XBD=2020 XBD=2097 XBD=2287 XBD=2353 XBD=2439 XBD=2539 XHG=1371 XHG=1578 XHG=1751 XHG=1853 XHG=1962 XHG=2045 XHG=2121 XHG=2312 XHG=2375 XHG=2461 YBD=896 YBD=889 YBD=890 YHG=859>infective YHG=868>process YHG=859>and YHG=860>how YHG=860>far YHG=869>an YHG=860>increase YHG=860>in YHG=858>the YHG=861>anti" XBD=158 XBD=229 XBD=279 XBD=371 XHG=111 XHG=175 XHG=245 XHG=294 YBD=896 YBD=900 YHG=872>1-0 YHG=871>ml. YHG=872>of YHG=871>O.A. YHG=872>(5) " XBD=676 XBD=157 XBD=228 XBD=279 XBD=441 XBD=617 XHG=110 XHG=174 XHG=245 XHG=291 XHG=453 XHG=633 YBD=926 YBD=930 YBD=925 YBD=929 YHG=901>1’0 YHG=901>ml. YHG=902>of YHG=901>egg-yolk YHG=900>substrate YHG=900>(3) XBD=1663 XHG=2460 XBD=1865 XBD=1943 XBD=2062 XBD=2227 XBD=2427 XBD=2540 XHG=1370 YBD=931 XHG=1699 XHG=1905 XHG=1980 XHG=2100 XHG=2263 YBD=935 YHG=899>their YBD=936 YBD=928 YBD=930 YBD=929 YHG=898>hyaluronidase YHG=898>activity YHG=899>of YHG=909>sera YHG=899>would YHG=899>enhance " XBD=1739 XBD=1582 XHG=1598 XHG=1371 YBD=974 YHG=937>action. YBD=968 YHG=937>protective 20-40 " XBD=1310 XBD=280 XHG=894 XBD=356 XBD=595 XBD=657 XBD=788 XBD=871 XBD=955 XBD=1052 XHG=106 XHG=301 XHG=509 XHG=619 XHG=680 XHG=813 XHG=990YBD=999 XHG=1074 YBD=993 YBD=994 YBD=995 YBD=994 YBD=1003 YHG=967>Incubate YHG=966>for YHG=966>min. YHG=968>at YHG=966>about YHG=966>37° YHG=966>C. YHG=966>the YHG=969>temperature ; " XBD=197 XBD=277 XBD=337 XBD=516 XBD=606 XBD=705 XBD=764 XBD=912 XBD=976 XBD=1054 XBD=1188 XBD=1309 XHG=107 XHG=213 XHG=294 XHG=354 XHG=534 XHG=621 XHG=722 XHG=780 XHG=928 XHG=992 XHG=1070 XHG=1236 YBD=1034 YBD=1032 YBD=1033 YBD=1041 YBD=1033 YBD=1041 YBD=1034 YHG=1006>need YHG=1009>not YHG=1006>be YHG=1007>accurate YHG=1004>and YHG=1013>may YHG=1005>be YHG=1005>judged YHG=1005>by YHG=1005>the YHG=1006>finger. YHG=1006>The " XBD=1891 XBD=1985 XBD=2269 XHG=1678 XHG=1909 XHG=2006 YBD=1057 YBD=1050 YBD=1051 YHG=1021>Summary YHG=1020>and YHG=1007>Conclusions follows:" XBD=596 XBD=233 XHG=557 XBD=309 XBD=541 XHG=107 YBD=1072 XHG=250 XHG=325 YBD=1074 YBD=1073 YBD=1080 YHG=1045>results YHG=1042>are YHG=1044>interpreted YHG=1052>as

with

"" XBD=2539 XBD=1497 XBD=1662 XBD=1726 XBD=1935 XBD=2214 XHG=1419 XHG=1524 XHG=1688 XHG=1750 XHG=1959 XHG=2359 YBD=1104YHG=1098>Tube YBD=1105 YBD=1113 YHG=1075>The YHG=1075>results YHG=1075>of YHG=1074>infection YHG=1074>experiments YHG=1075>organisms XBD=877 XHG=789 XBD=1025 XHG=890YBD=1122 YBD=1122 YHG=1097>number

" XBD=2540XHG=821 XBD=1411 XBD=1500 XBD=1793 XBD=1935 XBD=2020 XBD=2250 XHG=1372 XHG=1432 XHG=1524 XHG=1816 XHG=1957 XHG=2045 XHG=2299 XHG=2365 YBD=1146 YBD=1152 YBD=1144 YBD=1145 YBD=1147 YHG=1114>of YHG=1113>the YHG=1123>gas-gangrene YHG=1123>group YHG=1124>are YHG=1114>described. YHG=1115>In YHG=1114>infections " XBD=2346 XBD=561 XBD=619 XBD=848 XBD=1078 XBD=1278 XHG=556 XHG=605 XHG=1037 XHG=1242 YBD=1150 YBD=1149 YBD=1151 YBD=1152 YBD=1153 YHG=1143>. YHG=1131>I YHG=1132>n YHG=1132>III YHG=1133>IV " XBD=258 XBD=503 XHG=108 XHG=284 YBD=1184 YHG=1155>Actively YHG=1155>proliferating " XBD=1512 XBD=1598 XBD=1843 XBD=1965 XBD=2163 XBD=2488 XBD=2540 XHG=2516 XHG=1372 XHG=1547 XHG=1633 XHG=1875 XHG=2000 XHG=2199 YBD=1183 YBD=1191 YBD=1186 YHG=1153>caused YHG=1154>by YHG=1154>organisms YHG=1153>that YHG=1154>produce YHG=1154>hyaluronidase, YHG=1156>it trace " XBD=656 XBD=706 XBD=761 XBD=845 XBD=1053 XBD=1102 XBD=1147 XBD=1203 XBD=1267 XHG=622 XHG=679 XHG=733 XHG=829 XHG=915 XHG=1066 XHG=1121 XHG=1174 XHG=1250 YBD=1209 YBD=1212 YBD=1211 YBD=1211 YBD=1211 YBD=1213 YBD=1212 YHG=1184>to YHG=1188>+ YHG=1204>.. YHG=1187>0 YHG=1186>.. YHG=1186>to YHG=1188>+ YHG=1205>.. YHG=1188>0 trace XBD=198 XBD=330 XBD=497 XHG=152 XHG=213 XHG=346 YBD=1210 YHG=1185>Cl. YHG=1185>welchii YHG=1202>.... " XBD=1532 XBD=1639 XBD=1736 XBD=1909 XBD=2001 XBD=2064 XBD=2261 XBD=2318 XBD=2399 XHG=1371 XHG=1549 XHG=1656 XHG=1753 XHG=1929 XHG=2018 XHG=2084 XHG=2277 XHG=2333 XHG=2418 YBD=1225 YBD=1229 YBD=1230 YBD=1222 YBD=1223 YHG=1202>appears YHG=1192>that YHG=1193>this YHG=1202>enzyme YHG=1202>can YHG=1193>be YHG=1192>detected YHG=1193>in YHG=1193>the YHG=1194>oedema " XBD=2539 XBD=259 XHG=285 XBD=504 XHG=109 YBD=1243 YBD=1244 YHG=1215>Actively YHG=1214>proliferating " XBD=1466 XBD=1520 XBD=1628 XBD=1682 XBD=1882 XBD=1967 XBD=2025 XBD=2223 XBD=2294 XHG=1372 XHG=1478 XHG=1534 XHG=1639 XHG=1695 XHG=1896 XHG=1980 XHG=2041 XHG=2235 XHG=2307 YBD=1261 YBD=1262 YBD=1265 YHG=1232>fluid YHG=1241>soon YHG=1240>as YHG=1231>sufficient YHG=1241>can YHG=1233>be YHG=1232>collected YHG=1232>for YHG=1232>examination " XBD=2540 XBD=197 XHG=732 XBD=406 XBD=496 XBD=658 XBD=940 XBD=1065 XBD=1203 XBD=1266 XHG=152 XHG=214 XHG=468 XHG=570 XHG=1048 XHG=1174 XHG=1250 YBD=1270 YBD=1269 YBD=1271 YBD=1271 YBD=1270 YHG=1245>OZ. YHG=1244>cedematiens YHG=1262>.. YHG=1244>trace YHG=1246>.. YHG=1246>0trace.. YHG=1263>.. YHG=1247>0

" XBD=1065 XHG=1048 XHG=1250 YBD=1300 YHG=1276>0 . 0 .. .. XBD=211 XBD=497 XBD=621 XHG=110 XHG=223 YBD=1299 YHG=1274>Other YHG=1273>infections.. YHG=1274>0 " XBD=1266 XBD=1450XHG=605 XBD=1505 XBD=1583 XBD=1738 XBD=1796 XBD=1905 XBD=1959 XBD=2039 XBD=2206 XBD=2306 XBD=2358 XBD=2538 XHG=1370 XHG=1462 XHG=1517 XHG=1598 XHG=1753 XHG=1811 XHG=1917 XHG=1972 XHG=2054 XHG=2220 XHG=2319 XHG=2370 YBD=1300 YBD=1309 YBD=1301 YBD=1305 YHG=1271>and YHG=1271>in YHG=1271>muscle YHG=1280>soon YHG=1280>as YHG=1271>the YHG=1271>earliest YHG=1271>sign YHG=1271>of YHG=1271>infection.

|>" XBD=212 XBD=255 XBD=447 XBD=674 XBD=770 XBD=1008 XBD=1093 XBD=1238 XBD=965 XHG=185 XHG=229 XHG=268 XHG=466 XHG=686 XHG=991 XHG=1019 XHG=1108 XHG=783 YBD=1338 YBD=1330 YBD=1341 YBD=1340 YBD=1336 YBD=1337 YBD=1337 YHG=1314>+ YHG=1321>= YHG=1310>turbidity; YHG=1311>trace==very YHG=1311>faint YHG=1312>0 YHG=1312>turbidity ; YHG=1319>=no YHG=1312>change. " XBD=1834 XBD=1935 XBD=2167 XBD=2242 XBD=2443 XBD=2501 XBD=2539 XHG=1706 XHG=1862 XHG=1961 XHG=2196 XHG=2273 XHG=2466 XHG=2523 YBD=1346 YBD=1339 YBD=1347 YBD=1340 YBD=1341 YBD=1342 YHG=1310>toxin) YHG=1319>can YHG=1310>ordinarily YHG=1311>be YHG=1310>detected YHG=1312>at YHG=1322>a XBD=1634 XHG=1371 YBD=1343 YHG=1309>Lecithinase "" XBD=2338 XBD=1516 XBD=1642 XBD=1702 XBD=1920 XBD=2009 XBD=2071 XBD=2146 XHG=1370 XHG=1534 XHG=1660 XHG=1717 XHG=1938 XHG=2027 XHG=2092 XHG=2166 YBD=1380 YBD=1379 YBD=1387 YHG=1349>similar YHG=1349>stage YHG=1346>in YHG=1349>infections YHG=1349>due YHG=1349>to YHG=1349>Cl. YHG=1350>welchii. XBD=210 XBD=304 XBD=422 XBD=535 XBD=595 XBD=791 XBD=879 XBD=944 XBD=1033 XBD=1087 XBD=1124 XBD=1224 XBD=1312 XHG=152 XHG=224 XHG=319 XHG=438 XHG=550 XHG=611 XHG=806 XHG=896 XHG=961 XHG=1050 XHG=1102 XHG=1139 XHG=1240 YBD=1398 YBD=1400 YBD=1399 YBD=1401 YBD=1408 YBD=1400 YBD=1400 YBD=1401 YHG=1371>All YHG=1370>four YHG=1371>tubes YHG=1374>must YHG=1371>be YHG=1371>inspected YHG=1372>side YHG=1373>by YHG=1371>side YHG=1372>in YHG=1381>a YHG=1373>rack YHG=1372>and " XBD=1457 XBD=1623 XBD=1692 XBD=2015 XBD=2186 XBD=2397 XBD=2501 XBD=2540 XHG=1421 XHG=1484 XHG=1653 XHG=1719 XHG=2045 XHG=2214 XHG=2423 XHG=2523 YBD=1427 YBD=1419 YBD=1420 YBD=1421 YHG=1390>A YHG=1398>survey YHG=1389>of YHG=1389>representative YHG=1389>strains YHG=1389>indicates YHG=1390>that YHG=1400>a " XBD=170 XBD=446 XBD=664 XBD=786 XBD=969 XBD=1030 XBD=1221 XBD=1312 XHG=106 XHG=190 XHG=482 XHG=685 XHG=806 XHG=991 XHG=1047 XHG=1242 YBD=1447 YBD=1439 YBD=1440 YBD=1439 YBD=1448 YBD=1441 YHG=1413>not YHG=1410>individually YHG=1411>otherwise YHG=1411>small YHG=1415>amounts YHG=1411>of YHG=1412>turbidity YHG=1412>will ; " XBD=1473 XBD=1723 XBD=1788 XBD=1870 XBD=2036 XBD=2275 XBD=2394 XBD=2540 XHG=1370 XHG=1501 XHG=1749 XHG=1817 XHG=1899 XHG=2061 XHG=2299 XHG=2419 YBD=1466 YBD=1458 YBD=1459 YBD=1460 YHG=1429>large YHG=1428>proportion YHG=1428>of YHG=1428>Cl. YHG=1428>welchii YHG=1429>associated YHG=1428>with YHG=1429>clinical " XBD=390 XBD=151 XHG=172 XHG=106 YBD=1479 YBD=1478 YHG=1449>overlooked. YHG=1451>be " XBD=2540 XBD=1642 XHG=2513 XBD=1827 XBD=2152 XBD=2261 XBD=2345 XBD=2495 XHG=1371 YBD=1499 XHG=1664 XHG=1849 XHG=2198 XHG=2279 XHG=2366 YBD=1505 YHG=1470>of YBD=1506 YBD=1504 YBD=1497 YBD=1498 YHG=1477>gas-gangrene YHG=1468>produce YHG=1468>hyaluronidase. YHG=1468>All YHG=1467>the YHG=1467>strains " YBD=1535 YHG=1504>Discussion " XBD=831 XBD=1891 XBD=2094 XBD=2215 XBD=2420 XBD=2540 XHG=1688 XHG=1931 XHG=2134 XHG=2254 XHG=2480 YBD=1537 YBD=1536 YBD=1544 YBD=1539 YHG=1507>examined YHG=1507>produce YHG=1506>this YHG=1516>enzyme. YHG=1509>The XBD=1429XHG=601 XHG=1374 YBD=1538 YHG=1507>Cl. " XBD=1541 XBD=1651 XBD=1722 XBD=1969 XBD=2015 XBD=2355 XBD=2452 XHG=1371 XHG=1555 XHG=1670 XHG=1742 XHG=1981 XHG=2031 XHG=2383 XHG=2464 YBD=1576 YBD=1584 YBD=1577 YBD=1578 YHG=1546>position YHG=1546>with YHG=1546>Cl. YHG=1545>cedematiens YHG=1547>is YHG=1546>unsatisfactory; YHG=1546>less YHG=1546>than " XBD=2540 XBD=356 XHG=898 XBD=446 XBD=611 XBD=811 XBD=876 XBD=978 XBD=1118 XBD=1309 XHG=160 XHG=379 XHG=471 XHG=633 XHG=834 XHG=1002YBD=1590 XHG=1142 YBD=1597 YBD=1589 YBD=1598 YBD=1598YHG=1559>this YBD=1590 YHG=1558>Although YHG=1559>the YHG=1560>results YHG=1560>reported YHG=1560>in YHG=1569>paper YHG=1560>indicate " XBD=1453 XBD=1537 XBD=1698 XBD=1756 XBD=1832 XBD=2081 XBD=2301 XBD=2473 XBD=2539 XHG=1370 XHG=1470 XHG=1557 XHG=1717 XHG=1778 XHG=1854 XHG=2098 XHG=2318 XHG=2488 YBD=1624 YBD=1616 YHG=1586>half YHG=1586>strains YHG=1586>of YHG=1585>Cl. YHG=1586>cedemutiens YHG=1586>examined YHG=1586>produce YHG=1586>the " XBD=1309 XBD=199 XHG=917 XBD=395 XBD=457 XBD=661 XBD=805 XBD=896 XBD=962 XBD=1163 XBD=1223 XHG=108 XHG=219 XHG=417 XHG=478 XHG=682 XHG=825 XHG=986YBD=1629 XHG=1181 XHG=1241 YBD=1628 YBD=1636 YBD=1630 YBD=1629 YBD=1630 YHG=1598>that YHG=1608>enzymes YHG=1599>of YHG=1599>bacterial YHG=1600>origin YHG=1609>can YHG=1600>be YHG=1600>detected YHG=1599>in YHG=1599>the " XBD=1528 XBD=1631 XBD=1758 XBD=1815 XBD=1889 XBD=2090 XBD=2204 XBD=2308 XBD=2499 XBD=2540 XHG=1369 XHG=1552 XHG=1652 XHG=1781 XHG=1839 XHG=1913 XHG=2113 XHG=2227 XHG=2332 XHG=2517 YBD=1662 YBD=1655 YHG=1634>enzyme YHG=1626>and YHG=1626>there YHG=1625>is YHG=1635>no YHG=1625>evidence YHG=1625>that YHG=1625>this YHG=1625>property YHG=1626>is " XBD=228 XBD=341 XBD=401 XBD=472 XBD=593 XBD=720 XBD=779 XBD=982 XBD=1077 XBD=1184 XBD=1249 XBD=1310 XHG=107 XHG=248 XHG=358 XHG=419 XHG=489 XHG=612 XHG=740 XHG=796 XHG=997 XHG=1093 XHG=1204 XHG=1271 YBD=1668 YBD=1676 YBD=1669 YBD=1669 YBD=1677 YBD=1668 YBD=1669 YHG=1638>tissue YHG=1638>fluid YHG=1638>at YHG=1647>an YHG=1638>early YHG=1638>stage YHG=1639>of YHG=1638>infection YHG=1639>and YHG=1648>may YHG=1639>be YHG=1639>of " XBD=1580 XBD=1691 XBD=1772 XBD=1979 XBD=2036 XBD=2323 XBD=2412 XBD=2467 XBD=2541 XHG=1371 XHG=1594 XHG=1705 XHG=1788 XHG=1998 XHG=2053 XHG=2341 XHG=2428 XHG=2479 YBD=1695 YBD=1701 YBD=1693 YBD=1694 YHG=1664>correlated YHG=1664>with YHG=1665>the YHG=1664>incidence YHG=1664>of YHG=1673>gas-gangrene YHG=1664>due YHG=1664>to YHG=1665>this " XBD=1309 XBD=323 XHG=786 XBD=593 XBD=644 XBD=767 XBD=832 XBD=1090 XBD=1197 XHG=109 XHG=340 XHG=612 XHG=663 XHG=851YBD=1708 XHG=1107 XHG=1214 YBD=1714 YBD=1715 YBD=1707 YBD=1716 YBD=1708 YBD=1716 YHG=1678>diagnostic YHG=1677>significance, YHG=1678>it YHG=1678>must YHG=1678>be YHG=1678>emphasised YHG=1678>that YHG=1678>they " XBD=1574 XBD=1693 XBD=1930 XBD=2138 XBD=2205 XBD=2277 XBD=2507 XBD=2540 XHG=1369 XHG=1615 XHG=1708 XHG=1946 XHG=2153 XHG=2223 XHG=2295 XHG=2516 YBD=1741 YBD=1734 YBD=1733 YHG=1704>organism. YHG=1704>The YHG=1703>lecithinase YHG=1703>produced YHG=1704>by YHG=1703>Cl. YHG=1703>cedematiens YHG=1704>is " XBD=1308 XBD=208 XHG=774 XBD=287 XBD=412 XBD=623 XBD=748 XBD=959 XBD=1240 XHG=108 XHG=236 XHG=316 XHG=440 XHG=648 XHG=986YBD=1748 XHG=1267 YBD=1747 YBD=1755 YBD=1748 YHG=1717>have YHG=1717>all YHG=1718>been YHG=1717>obtained YHG=1717>from YHG=1717>infection YHG=1717>experiments YHG=1718>in " XBD=1408 XBD=1518 XBD=1603 XBD=1783 XBD=1887 XBD=1933 XBD=1979 XBD=2165 XBD=2223 XBD=2285 XBD=2340 XHG=1368 XHG=1423 XHG=1532 XHG=1619 XHG=1796 XHG=1900 XHG=1944 XHG=1994 XHG=2180 XHG=2239 XHG=2302 XHG=2356 YBD=1774 YBD=1772 YBD=1781 YBD=1773 YBD=1780 YHG=1744>such YHG=1743>low YHG=1743>potency YHG=1743>that YHG=1743>it YHG=1743>is YHG=1743>unlikely YHG=1744>to YHG=1743>be YHG=1743>of YHG=1743>diagnostic " XBD=2540 XBD=271 XHG=926 XBD=385 XBD=509 XBD=583 XBD=776 XBD=837 XBD=903 XBD=947 XBD=1094 XBD=1307 XHG=108 XHG=289 XHG=402 XHG=527 XHG=604 XHG=793 XHG=856 XHG=963YBD=1771 XHG=1116 YBD=1788 YBD=1787 YBD=1795 YBD=1793 YHG=1757>animals YHG=1757>with YHG=1757>what YHG=1767>we YHG=1757>believed YHG=1757>to YHG=1758>be YHG=1758>&dquo; YHG=1757>pure YHG=1757>cultures &dquo; ; " XBD=1307 XBD=1607XHG=763 XBD=1666 XBD=1745 XBD=1880 XBD=2021 XHG=1367 XHG=1624 XHG=1679 XHG=1761 XHG=1898 YBD=1820 YBD=1812 YBD=1813 YHG=1783>significance YHG=1783>in YHG=1783>the YHG=1783>tissue YHG=1782>fluids. " XBD=215 XBD=430 XBD=554 XBD=683 XBD=744 XBD=880 XBD=1060 XBD=1178 XHG=107 XHG=228 XHG=449 XHG=576 XHG=703 XHG=894 XHG=1079 XHG=1196 YBD=1827 YBD=1826 YBD=1834 YBD=1834 YBD=1826 YHG=1796>these YHG=1797>infections YHG=1797>must YHG=1797>differ YHG=1797>in YHG=1806>many YHG=1796>respects YHG=1796>from YHG=1797>those " XBD=1593 XBD=1676 XBD=1763 XBD=1983 XBD=2044 XBD=2359 XBD=2456 XBD=2539 XHG=1417 XHG=1616 XHG=1697 XHG=1790 XHG=2006 XHG=2068 XHG=2382 XHG=2474 YBD=1852 YBD=1859 YBD=1851 YHG=1822>Methods YHG=1822>for YHG=1822>the YHG=1821>detection YHG=1822>of YHG=1821>hyaluronidase YHG=1822>and YHG=1821>leci" XBD=292 XBD=404 XBD=510 XBD=566 XBD=753 XBD=925 XBD=1063 XBD=1140 XHG=103 XHG=305 XHG=420 XHG=525 XHG=581 XHG=767 XHG=939 XHG=1077 XHG=1155 YBD=1874 YBD=1873 YBD=1866 YBD=1866 YBD=1866 YBD=1873 YHG=1836>resulting YHG=1836>from YHG=1836>civil YHG=1845>or YHG=1836>military YHG=1836>wounds YHG=1836>which YHG=1845>are YHG=1836>usually "" XBD=1307 XBD=1520 XBD=1660 XBD=1748 XBD=1808 XBD=1919 XBD=1974 XBD=2054 XBD=2160 XBD=2270 XBD=2350 XBD=2539 XHG=1365 XHG=1535 XHG=1676 XHG=1762 XHG=1824 XHG=1933 XHG=1988 XHG=2072 XHG=2174 XHG=2284 XHG=2366 YBD=1892 YBD=1891 YBD=1890 YHG=1861>thinase YHG=1861>which YHG=1871>can YHG=1861>be YHG=1862>used YHG=1862>in YHG=1862>the YHG=1852>field YHG=1860>with YHG=1861>the YHG=1860>minimum XBD=397 XHG=749 XBD=518 XBD=567 XBD=722 XBD=929 XBD=1060 XBD=1180 XBD=1306 XHG=110 XHG=421 XHG=543 XHG=593 XHG=956YBD=1905 XHG=1112 XHG=1207 YBD=1906 YBD=1907 YBD=1906 YBD=1905 YHG=1875>contaminated YHG=1875>with YHG=1885>a YHG=1875>mixed YHG=1875>bacterial YHG=1875>flora. YHG=1875>We YHG=1876>have " XBD=1407 XBD=1643 XBD=1732 XBD=1967 XBD=2164 XBD=2368 XBD=2433 XBD=2539 XHG=1368 XHG=1434 XHG=1671 XHG=1764 XHG=2022 XHG=2193 XHG=2396 XHG=2458 YBD=1931 YBD=1938 YBD=1930 YBD=1929 YBD=1936 YHG=1900>of YHG=1900>apparatus YHG=1910>are YHG=1901>described. YHG=1900>Simple YHG=1900>methods YHG=1899>of YHG=1899>diag" XBD=1305 XBD=179 XHG=763 XBD=236 XBD=309 XBD=401 XBD=481 XBD=748 XBD=803 XBD=1035 XBD=1124 XHG=108 XHG=193 XHG=250 XHG=322 XHG=414 XHG=498 XHG=817YBD=1945 XHG=1048 XHG=1139 YBD=1952 YBD=1952 YBD=1952 YBD=1945 YHG=1915>not YHG=1924>so YHG=1915>far YHG=1915>had YHG=1915>the YHG=1915>opportunity YHG=1915>of YHG=1914>examining YHG=1924>any YHG=1914>exudate "" XBD=2539 XBD=1504 XBD=1590 XBD=1795 XBD=1945 XBD=2003 XBD=2090 XBD=2289 XBD=2363 XHG=1366 XHG=1524 XHG=1613 XHG=1816 XHG=1965 XHG=2024 XHG=2112 XHG=2311 XHG=2380 YBD=1971 YBD=1970 YBD=1977 YBD=1969 YHG=1940>nosing YHG=1940>bacterial YHG=1949>source YHG=1940>of YHG=1940>the YHG=1947>enzymes YHG=1939>by YHG=1937>means of XBD=153 XHG=866 XBD=307 XBD=475 XBD=588 XBD=759 XBD=851 XBD=938 XBD=1136 XBD=1306 XHG=111 XHG=167 XHG=323 XHG=487 XHG=601 XHG=773 XHG=950YBD=1984 XHG=1152 YBD=1985 YBD=1986 YBD=1984 YBD=1992 YHG=1954>of YHG=1955>muscle YHG=1954>excised YHG=1954>from YHG=1955>wounds YHG=1954>and YHG=1963>can YHG=1953>therefore YHG=1963>express "" XBD=2540 XBD=1516 XBD=1852 XBD=1991 XBD=2130 XBD=2339 XBD=2441 XHG=1365 XHG=1560 XHG=1892 XHG=2032 XHG=2172 XHG=2381 XHG=2479 YBD=2017 YBD=2010 YBD=2009 YBD=2008 YHG=1980>specific YHG=1979>neutralisation YHG=1979>tests YHG=1979>with YHG=1979>antisera YHG=1988>are YHG=1977>also XBD=160 XBD=336 XBD=396 XBD=458 XBD=562 XBD=639 XBD=727 XBD=1018 XBD=1254 XBD=1306 XHG=109 XHG=180 XHG=353 XHG=413 XHG=476 XHG=580 XHG=659 XHG=746 XHG=1035 XHG=1272 YBD=2032 YBD=2025 YBD=2024 YBD=2032 YBD=2031 YBD=2023 YHG=2004>no YHG=1994>opinion YHG=2003>as YHG=1995>to YHG=1995>how YHG=1994>far YHG=2004>our YHG=1993>experimental YHG=1994>experience YHG=1994>is " XBD=1573 XHG=1366 YBD=2051 YHG=2019>described. " XBD=1135 XBD=224 XHG=788 XBD=293 XBD=367 XBD=602 XBD=668 XBD=760 XHG=105 XHG=249 XHG=320 XHG=396 XHG=627 XHG=693 XHG=939YBD=2064 YBD=2072 YBD=2065 YBD=2063 YBD=2071YHG=2033>field. YHG=2034>likely YHG=2034>to YHG=2035>be YHG=2034>confirmed YHG=2034>in YHG=2034>the YHG=2034>Although experi"" XBD=887 XBD=1455 XBD=1507 XBD=1729 XBD=1837 XBD=1962 XBD=2257 XBD=2411 XBD=2541 XHG=1416 XHG=1473 XHG=1527 XHG=1747 XHG=1855 XHG=1982 XHG=2275 XHG=2429 YBD=2089 YBD=2096 YBD=2088 YBD=2097 YBD=2090 YBD=2087 YHG=2059>It YHG=2059>is YHG=2059>suggested YHG=2058>that YHG=2059>these YHG=2059>experimental YHG=2058>results YHG=2056>should XBD=254 XHG=689 XBD=456 XBD=666 XBD=779 XBD=1093 XBD=1196 XBD=1306 XHG=109 XHG=278 XHG=481 XHG=804YBD=2103 XHG=1117 XHG=1219 YBD=2104 YBD=2111 YBD=2103 YHG=2074>mental YHG=2074>evidence YHG=2073>indicates YHG=2073>that YHG=2073>hyaluronidase YHG=2073>and YHG=2073>leci" XBD=1306 XBD=1412XHG=799 XBD=1589 XBD=1653 XBD=1739 XBD=2025 XBD=2085 XBD=2255 XBD=2446 XBD=2542 XHG=1366 XHG=1434 XHG=1609 XHG=1672 XHG=1761 XHG=2046 XHG=2107 XHG=2275 XHG=2463 YBD=2136 YBD=2129 YBD=2128 YBD=2125 YHG=2098>be YHG=2098>applied YHG=2098>to YHG=2099>the YHG=2099>examination YHG=2098>of YHG=2099>clinical YHG=2097>material YHG=2097>from " XBD=259 XBD=356 XBD=424 XBD=625 XBD=687 XBD=773 XBD=945 XBD=1061 XBD=1124 XBD=1241 XHG=106 XHG=283 XHG=378 XHG=449 XHG=646 XHG=707 XHG=968 XHG=1082 XHG=1146 XHG=1262 YBD=2143 YBD=2142 YBD=2143 YBD=2143 YBD=2142 YHG=2113>thinase YHG=2123>can YHG=2113>be YHG=2113>detected YHG=2113>in YHG=2113>the YHG=2113>oedema YHG=2113>fluid YHG=2121>as YHG=2122>soon YHG=2122>as

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seticum

""|>"XBD=2541 XBD=1525 XBD=1602 XBD=1704 XBD=1840 XBD=1965 XBD=2095 XBD=2224 XBD=2408 XHG=1365 XHG=1561 XHG=1637 XHG=1741 XHG=1875 XHG=2001 XHG=2132 XHG=2259 XHG=2440 YBD=2168 YBD=2176 YBD=2175 YBD=2169 YBD=2166 YHG=2137>wounds YHG=2137>in YHG=2138>the YHG=2139>hope YHG=2138>that YHG=2138>they YHG=2147>may YHG=2137>furnish YHG=2135>useful XBD=215 XBD=268 XBD=542 XBD=696 XBD=774 XBD=1067 XBD=1143 XBD=1215 XBD=1307 XBD=478 XHG=107 XHG=235 XHG=497 XHG=564 XHG=716 XHG=794 XHG=1087 XHG=1166 XHG=1236 XHG=289 YBD=2185 YBD=2183 YBD=2182 YBD=2188 YBD=2183 YBD=2182 YHG=2153>there YHG=2153>is YHG=2153>to YHG=2152>collect YHG=2153>for YHG=2152>examination, YHG=2153>sufficient YHG=2161>we YHG=2152>do YHG=2152>not "" XBD=2541 XBD=1581 XBD=1848 XBD=1908 XBD=1977 XBD=2098 XBD=2225 XBD=2284 XBD=2367 XHG=1366 XHG=1599 XHG=1865 XHG=1926 XHG=1995 XHG=2117 XHG=2246 XHG=2301 XHG=2385 YBD=2214 YBD=2215 YBD=2207 YBD=2206 YBD=2208 YHG=2177>diagnostic YHG=2177>information YHG=2178>at YHG=2188>an YHG=2177>early YHG=2177>stage YHG=2177>of YHG=2177>the YHG=2175>infection XBD=222 XHG=849 XBD=321 XBD=438 XBD=559 XBD=748 XBD=839 XBD=990 XBD=1045 XBD=1123 XBD=1307 XHG=110 XHG=235 XHG=334 XHG=451 XHG=573 XHG=761 XHG=1003 XHG=1056 XHG=1137 YBD=2223 YBD=2230 YBD=2222 YBD=2229 YBD=2221 YHG=2192>know YHG=2194>how YHG=2192>early YHG=2192>these YHG=2201>enzymes YHG=2192>will YHG=2201>appear YHG=2192>in YHG=2192>the " XBD=2515 XBD=1444 XHG=2292 XBD=1553 XBD=1687 XBD=1801 XBD=1980 XBD=2076 XBD=2276 XHG=1365 YBD=2246 XHG=1461 XHG=1571 XHG=1704 XHG=1819 XHG=1997 XHG=2092 YBD=2255 YHG=2191>infected YBD=2247 YBD=2254 YHG=2217>thus YHG=2216>assist YHG=2217>both YHG=2217>surgical YHG=2217>and YHG=2218>ancillary YHG=2215>treatments. " XBD=1229 XBD=1305XHG=759 XBD=249 XBD=322 XBD=437 XBD=535 XBD=659 XBD=730 XBD=859 XBD=1121 XHG=108 XHG=280 XHG=352 XHG=467 XHG=564 XHG=692 XHG=889YBD=2260 XHG=1151 XHG=1259 YBD=2263 YBD=2262 YBD=2261 YBD=2268 YBD=2261 YHG=2232>wound YHG=2241>or YHG=2232>how YHG=2232>the YHG=2231>time YHG=2231>of YHG=2231>their YHG=2240>appearance YHG=2231>will YHG=2231>be " XBD=1307 XBD=319 XHG=699 XBD=455 XBD=657 XBD=846 XBD=989 XBD=1070 XHG=886 XHG=110 XHG=359 XHG=498 XHG=1031 XHG=1110 YBD=2300 YBD=2301 YBD=2307 YBD=2300 YBD=2301 YHG=2271>correlated YHG=2271>with YHG=2271>obvious YHG=2271>clinical YHG=2271>signs YHG=2270>of YHG=2270>infection. " XBD=1306 XBD=265 XHG=874 XBD=548 XBD=730 XBD=846 XBD=954 XBD=1014 XBD=1157 XBD=1229 XHG=110 XHG=293 XHG=577 XHG=757 XHG=981YBD=2339 XHG=1041 XHG=1186 XHG=1259 YBD=2340 YBD=2347 YBD=2346 YBD=2339 YBD=2347 YBD=2340 YBD=2339 YHG=2310>Animal YHG=2310>experiments YHG=2309>suggest YHG=2309>that YHG=2309>this YHG=2309>is YHG=2309>likely YHG=2310>to YHG=2309>be

" XBD=1118 XBD=1307XHG=872 XBD=216 XBD=281 XBD=369 XBD=574 XBD=747 XBD=848 XBD=997 XHG=110 XHG=239 XHG=302 XHG=393 XHG=599 XHG=770 XHG=1022 XHG=1143 YBD=2387 YBD=2379 YBD=2386 YBD=2378 YBD=2378 YBD=2385 YHG=2349>early YHG=2349>in YHG=2349>the YHG=2349>infective YHG=2357>process YHG=2349>and YHG=2348>before YHG=2348>local YHG=2349>changes " XBD=1306 XBD=174 XHG=733 XBD=426 XBD=519 XBD=708 XBD=776 XBD=849 XBD=1021 XBD=1213 XHG=804 XHG=109 XHG=203 XHG=480 XHG=544 XHG=877YBD=2417 XHG=1045 XHG=1239 YBD=2418 YBD=2426 YBD=2425 YBD=2418 YBD=2417 YHG=2397>are YHG=2388>diagnostic. YHG=2388>It YHG=2388>remains YHG=2387>to YHG=2388>be YHG=2387>proved YHG=2387>whether YHG=2388>the

REFERENCES

" XBD=1239 XBD=1309XHG=886 XBD=192 XBD=310 XBD=405 XBD=620 XBD=857 XBD=949 XHG=110 XHG=221 XHG=338 XHG=435 XHG=649 XHG=980YBD=2457 XHG=1266 YBD=2458 YBD=2464 YBD=2464 YBD=2456 YHG=2427>fact YHG=2427>that YHG=2427>the YHG=2427>infecting YHG=2427>organisms YHG=2436>are YHG=2427>proliferating YHG=2427>in " XBD=1307 XBD=300 XHG=556 XBD=489 XBD=544 XBD=720 XBD=838 XBD=1026 XBD=1078 XBD=1155 XHG=732 XHG=110 XHG=311 XHG=500 XHG=851YBD=2496 XHG=1037 XHG=1088 XHG=1166 YBD=2497 YBD=2504 YBD=2504 YBD=2495 YBD=2496 YHG=2466>sufficient YHG=2466>numbers YHG=2466>to YHG=2466>produce YHG=2467>these YHG=2475>enzymes YHG=2466>in YHG=2466>the YHG=2466>tissues

1. 2. 3. 4. 5.

" XBD=1308 XBD=372 XHG=785 XBD=475 XBD=617 XBD=757 XBD=851 XBD=1092 XBD=1185 XHG=111 XHG=402 XHG=506 XHG=646 XHG=883YBD=2575 XHG=1122 XHG=1217 YBD=2583 YBD=2582 YBD=2574YHG=2545>the YBD=2582 YHG=2544>proliferation YHG=2554>can YHG=2554>occur YHG=2539>when YHG=2544>organisms YHG=2554>are YHG=2545>only

6. 7. 8.

" XBD=1309 XBD=144 XHG=758 XBD=348 XBD=416 XBD=567 XBD=733 XBD=941 XBD=1008 XBD=1202 XHG=110 XHG=171 XHG=377 XHG=441 XHG=594 XHG=967YBD=2535 XHG=1034 XHG=1227 YBD=2536 YBD=2535 YBD=2536 YHG=2505>is YHG=2505>evidence YHG=2506>of YHG=2506>active YHG=2505>wound YHG=2505>infection YHG=2515>or YHG=2505>whether YHG=2505>this

" XBD=1308 XBD=264 XHG=626 XBD=337 XBD=596 XBD=809 XBD=1136 XHG=112 XHG=295 XHG=368 XHG=840YBD=2614 XHG=1193 YBD=2621 YBD=2613 YBD=2622 YBD=2614 YBD=2614 YHG=2583>present YHG=2593>as YHG=2584>apparently YHG=2584>harmless YHG=2584>contaminants. YHG=2584>From " XBD=1125 XBD=1309XHG=838 XBD=181 XBD=421 XBD=541 XBD=680 XBD=817 XBD=937 XHG=111 XHG=205 XHG=445 XHG=561 XHG=705 XHG=958YBD=2653 XHG=1146 YBD=2660 YBD=2654 YBD=2653 YBD=2654 YHG=2632>our YHG=2623>experience YHG=2624>with YHG=2624>tissue YHG=2624>fluids YHG=2623>from YHG=2623>infected YHG=2624>animals " XBD=1306 XBD=167 XHG=691 XBD=262 XBD=481 XBD=659 XBD=781 XBD=878 XBD=1087 XBD=1197 XHG=813 XHG=110 XHG=202 XHG=296 XHG=516 XHG=911YBD=2692 XHG=1118 XHG=1207 YBD=2697 YBD=2698 YBD=2695 YBD=2693 YBD=2693 YHG=2671>we YHG=2662>do, YHG=2662>however, YHG=2662>believe YHG=2663>that YHG=2663>the YHG=2663>methods YHG=2672>we YHG=2664>have " XBD=1307 XBD=399 XHG=873 XBD=504 XBD=583 XBD=768 XBD=841 XBD=939 XBD=1236 XHG=109 XHG=432 XHG=536 XHG=616 XHG=799 XHG=973YBD=2731 XHG=1268 YBD=2732 YBD=2730 YBD=2738 YBD=2732 YBD=2732 YHG=2701>recommended YHG=2710>can YHG=2702>be YHG=2702>applied YHG=2702>to YHG=2702>the YHG=2701>examination YHG=2702>of " XBD=461 XBD=257 XHG=276 XHG=107 YBD=2769 YBD=2770 YHG=2739>material. YHG=2739>clinical

" " " "

XBD=1306XHG=890 XBD=330 XBD=408 XBD=575 XBD=877 XBD=986 XBD=1024 XBD=1140 XHG=160 XHG=346 XHG=425 XHG=593 XHG=1000YBD=2811 XHG=1039 XHG=1157 YBD=2808 YBD=2807 YBD=2816 YBD=2810 YBD=2809YHG=2780>with YBD=2817 YHG=2777>Wounds YHG=2787>are YHG=2779>usually YHG=2779>contaminated YHG=2789>a YHG=2780>large YHG=2780>variety XBD=150 XHG=880 XBD=388 XBD=484 XBD=565 XBD=712 XBD=861 XBD=919 XBD=1000 XBD=1200 XBD=1306 XHG=110 XHG=167 XHG=405 XHG=498 XHG=583 XHG=727 XHG=934YBD=2849 XHG=1017 XHG=1215 YBD=2854 YBD=2847 YBD=2848 YBD=2849 YBD=2849 YBD=2856 YHG=2817>of YHG=2817>organisms, YHG=2818>and YHG=2818>the YHG=2818>mixed YHG=2819>nature YHG=2819>of YHG=2819>the YHG=2819>infection YHG=2828>may XBD=292 XHG=754 XBD=371 XBD=489 XBD=613 XBD=727 XBD=960 XBD=1210 XBD=1304 XHG=107 XHG=318 XHG=396 XHG=513 XHG=638 XHG=988YBD=2894 XHG=1237 YBD=2885 YBD=2887 YBD=2895 YBD=2888 YHG=2857>influence YHG=2856>its YHG=2856>final YHG=2857>form YHG=2858>and, YHG=2867>moreover, YHG=2858>complicate YHG=2858>the XBD=301 XHG=900 XBD=430 XBD=512 XBD=803 XBD=871 XBD=967 XBD=1176 XBD=1307 XHG=108 XHG=330 XHG=460 XHG=541 XHG=834 XHG=998YBD=2926 XHG=1232 YBD=2934 YBD=2925 YBD=2927 YBD=2928 YBD=2928 YHG=2895>diagnosis YHG=2895>from YHG=2905>an YHG=2896>examination YHG=2898>of YHG=2897>the YHG=2897>exudate. YHG=2897>For

" XBD=1306 XBD=295 XHG=681 XBD=335 XBD=492 XBD=599 XBD=661 XBD=965 XBD=1032 XBD=1072 XHG=108 XHG=312 XHG=351 XHG=507 XHG=615 XHG=980YBD=2966 XHG=1048 XHG=1088 YBD=2973 YBD=2965 YBD=2972 YBD=2964 YBD=2973 YBD=2966YHG=2936>contaminated YBD=2974 YHG=2935>example, YHG=2944>a YHG=2935>wound YHG=2943>may YHG=2935>be YHG=2936>by YHG=2946>a YHG=2930>hyaluroni-

9. 10. 11.

12.

" XBD=1472 XBD=1522 XBD=1673 XBD=1730 XBD=1802 XBD=1912 XBD=1962 XBD=2013 XBD=2086 XBD=2245 XBD=2408 XBD=2454 XHG=1362 XHG=1488 XHG=1539 XHG=1689 XHG=1746 XHG=1815 XHG=1926 XHG=1977 XHG=2029 XHG=2103 XHG=2261 XHG=2423 XHG=2466 YBD=3383 YBD=3384 YBD=3386 YBD=3385 YHG=3360>action YHG=3361>of YHG=3359>calcium YHG=3366>on YHG=3359>the YHG=3350>’toxin YHG=3366>or YHG=3360>to YHG=3366>use YHG=3359>buffered YHG=3360>solutions YHG=3361>in YHG=3361>their " XBD=2529 XBD=272 XHG=685 XBD=386 XBD=477 XBD=660 XBD=763 XBD=1091 XBD=1156 XBD=1305 XHG=104 XHG=296 XHG=409 XHG=503 XHG=787YBD=3394 XHG=1115 XHG=1181 YBD=3392 YBD=3393 YBD=3401 YBD=3399 YBD=3391 YBD=3399 YHG=3362>indicate YHG=3363>that YHG=3365>the YHG=3373>enzyme YHG=3372>was YHG=3362>staphylococcal YHG=3362>in YHG=3362>origin

" XBD=1486 XBD=1599 XBD=1762 XBD=1862 XBD=2052 XBD=2229 XBD=2336 XHG=1361 XHG=1506 XHG=1620 XHG=1783 XHG=1882 XHG=2074 XHG=2272 XHG=2357 YBD=3416 YBD=3412 YBD=3413 YBD=3417 YBD=3414 YBD=3419 YHG=3389>system YHG=3388>when YHG=3388>working YHG=3388>with YHG=3388>undiluted YHG=3388>reagents. YHG=3389>The YHG=3390>non-specific clinical " XBD=2528 XBD=290 XHG=765 XBD=389 XBD=517 XBD=732 XBD=888 XBD=1019 XBD=1120 XHG=103 XHG=322 XHG=425 XHG=549 XHG=922YBD=3438 XHG=1053 YBD=3437 YBD=3432 YBD=3430 YBD=3429 YHG=3401>although YHG=3402>the YHG=3403>final YHG=3403>infection YHG=3401>might YHG=3402>have YHG=3400>the " XBD=2371 XBD=2526 XHG=2397 XBD=1606 XBD=1660 XBD=1853 XBD=1926 XBD=2007 XBD=2195 XBD=2312 XHG=1361 YBD=3445 XHG=1632 XHG=1687 XHG=1881 XHG=1952 XHG=2035 XHG=2222 XHG=2340 YBD=3446 YHG=3419>antitoxin YBD=3441 YBD=3442 YBD=3443 YHG=3417>neutralisation YHG=3417>is YHG=3417>explained YHG=3418>by YHG=3417>the YHG=3417>excessive YHG=3418>doses YHG=3419>of XHG=1362 YBD=3475 YHG=3447>employed. " XBD=1542 XBD=320 XHG=823 XBD=393 XBD=449 XBD=764 XBD=983 XBD=1121 XBD=1204 XBD=1302 XHG=105 XHG=355 XHG=425 XHG=482 XHG=1015 XHG=1154 XHG=1234 YBD=3470 YBD=3478 YBD=3469 YBD=3469 YHG=3439>characters YHG=3441>of YHG=3450>a YHG=3450>gas-gangrene. YHG=3439>Further YHG=3439>work YHG=3448>on YHG=3439>the

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J. Path. Bact. 52, 99. 13. McClean, D. (1930) Ibid, 33, 1045. 14. (1931) Ibid, 34, 459. 15. (1936) Ibid, 42, 477. 16. (1941a) Ibid, 53, 13. 17. (1941b) Ibid, 53, 156. 18. (1943) Biochem. J. in the press. 19. and Hale, C. W. (1941) Ibid, 35, 159. 20. Meyer, K., Chaffee, E., Hobby, G. L. and Dawson, M. H. (1941) J. exp. Med. 73, 309. 21. Oakley, C. L. and Warrack, G. H. (1941) J. Path. Bact. 53, 335. 22. van Heyningen, W. E. (1941) Biochem. J. 35, 1246. 23. Weed, L. A., Minton, S. and Carter, E. (1942) War Med. 2, 952.

" XBD=1304 XBD=426 XHG=872 XBD=614 XBD=750 XBD=833 XBD=938 XHG=110 XHG=466 XHG=657 XHG=790 XHG=980YBD=3005 YBD=3013 YBD=3010 YBD=3004 YBD=3013YHG=2975>the YHG=2974>dase-producing YHG=2975>aerobe, YHG=2975>such YHG=2984>as YHG=2976>staphylococcus, " XBD=1305 XBD=282 XHG=697 XBD=401 XBD=449 XBD=672 XBD=958 XBD=1150 XBD=1214 XHG=107 XHG=305 XHG=425 XHG=472 XHG=983YBD=3044 XHG=1175 XHG=1237 YBD=3051 YBD=3042 YBD=3050 YBD=3044 YBD=3044 YBD=3045 YHG=3014>together YHG=3013>with YHG=3022>a YHG=3012>relatively YHG=3014>non-invasive YHG=3015>member YHG=3014>of YHG=3015>the "" XBD=2533 XBD=378 XHG=857 XBD=527 XBD=688 XBD=781 XBD=841 XBD=923 XBD=1090 XBD=1224 XBD=1305 XHG=107 XHG=395 XHG=570 XHG=703 XHG=795 XHG=940YBD=3082 XHG=1133 XHG=1237 YBD=3091 YBD=3089 YBD=3081 YBD=3083 YBD=3083YHG=3053>the YHG=3062>gas-gangrene YHG=3061>group. YHG=3051>What YHG=3053>will YHG=3053>be YHG=3053>result YHG=3051>Will ? YHG=3053>the XBD=1425 XBD=1531 XBD=1616 XBD=1726 XBD=1812 XBD=1988 XBD=2060 XBD=2288 XBD=2414 XHG=1409 XHG=1439 XHG=1552 XHG=1638 XHG=1746 XHG=1835 XHG=2009 XHG=2081 XHG=2310 XHG=2434 YBD=3097 YBD=3093 YBD=3098 YBD=3099 YBD=3094 YHG=3070>t YHG=3070>Since YHG=3069>this YHG=3070>work YHG=3075>was YHG=3070>prepared YHG=3070>for YHG=3070>publication, YHG=3065>Weed, YHG=3070>Minton XBD=1429 XBD=1691 XBD=1813 XBD=1903 XBD=1971 XBD=2178 XBD=2227 XBD=2261 XBD=2428 XBD=2468 XHG=1365 XHG=1608 XHG=1706 XHG=1828 XHG=1917 XHG=1987 XHG=2193 XHG=2241 XHG=2274 XHG=2439 XHG=2479 YBD=3126 YBD=3122 YBD=3127 YBD=3123 YBD=3128 YHG=3100>and YHG=3098>have YHG=3099>stated YHG=3098>that YHG=3098>the YHG=3099>production YHG=3099>of YHG=3104>a YHG=3099>turbidity YHG=3100>in YHG=3104>egg" XBD=2533 XBD=237 XHG=388 XBD=291 XBD=372 XBD=694 XBD=1016 XBD=1072 XBD=1151 XBD=1306 XHG=103 XHG=252 XHG=305 XHG=709YBD=3129 XHG=1030 XHG=1085 XHG=1165 YBD=3122 YBD=3121 YBD=3128 YBD=3121 YHG=3092>action YHG=3092>of YHG=3092>the YHG=3090>staphylococcal YHG=3091>hyaluronidases YHG=3092>in YHG=3092>the YHG=3091>tissues " XBD=1444 XBD=1649 XBD=1713 XBD=1778 XBD=2058 XBD=2212 XBD=2258 XBD=2335 XBD=2532 XHG=1365 XHG=1463 XHG=1669 XHG=1733 XHG=1935 XHG=2077 XHG=2231 XHG=2277 XHG=2353 YBD=3156 YBD=3154 YBD=3153 YBD=3152 YBD=3155 YHG=3128>yolk YHG=3127>suspension YHG=3128>by YHG=3128>OZ. YHG=3127>culture YHG=3127>filtrates YHG=3128>is YHG=3127>not YHG=3127>attributable " XBD=239 XBD=322 XBD=609 XBD=818 XBD=874 XBD=1041 XBD=1109 XBD=1306 XHG=104 XHG=254 XHG=339 XHG=626 XHG=832 XHG=889 XHG=1057 XHG=1122 YBD=3161 YBD=3169 YBD=3167 YBD=3160 YBD=3161 YBD=3161 YHG=3132>enable YHG=3131>the YHG=3139>gas-gangrene YHG=3130>organism YHG=3130>to YHG=3130>initiate YHG=3140>an YHG=3131>infection " XBD=1400 XBD=1481 XBD=1520 XBD=1644 XBD=1777 XBD=1892 XBD=1988 XBD=2066 XBD=2231 XBD=2278 XBD=2357 XBD=2496 XBD=2532 XHG=1365 XHG=1424 XHG=1504 XHG=1543 XHG=1688 XHG=1799 XHG=1916 XHG=2011 XHG=2088 XHG=2251 XHG=2300 XHG=2378 XHG=2513 YBD=3179 YBD=3185 YBD=3181 YBD=3182 YBD=3188 YBD=3180 YHG=3158>to YHG=3157>the YHG=3167>a YHG=3157>toxin. YHG=3157>They YHG=3157>claim YHG=3156>that YHG=3156>the YHG=3156>reaction YHG=3157>not YHG=3157>specific, YHG=3157>is " XBD=185 XHG=865 XBD=280 XBD=365 XBD=594 XBD=786 XBD=847 XBD=932 XBD=1109 XBD=1303 XHG=104 XHG=204 XHG=298 XHG=387 XHG=613 XHG=809 XHG=953YBD=3199 XHG=1129 YBD=3201 YBD=3200 YBD=3207 YBD=3207 YBD=3207 YHG=3171>and YHG=3171>will YHG=3171>the YHG=3170>continued YHG=3178>presence YHG=3169>of YHG=3170>the YHG=3179>enzyme YHG=3170>promote " XBD=2532 XBD=1527 XBD=1599 XBD=1680 XBD=1829 XBD=1989 XBD=2099 XBD=2224 XBD=2418 XBD=2493 XHG=1364 XHG=1555 XHG=1624 XHG=1706 XHG=1855 XHG=2013 XHG=2124 XHG=2249 XHG=2440 XHG=2512 YBD=3209 YBD=3214 YBD=3215 YBD=3212 YBD=3210 YHG=3187>produced YHG=3186>by YHG=3186>the YHG=3187>culture YHG=3186>filtrates YHG=3186>from YHG=3191>many YHG=3187>organisms YHG=3188>and YHG=3186>is XBD=1558 XBD=1624 XBD=1769 XBD=1974 XBD=2084 XBD=2254 XBD=2306 XBD=2379 XBD=2493 XHG=1364 XHG=1580 XHG=1642 XHG=1790 XHG=2018 XHG=2103 XHG=2272 XHG=2324 XHG=2395 XHG=2507 YBD=3243 YBD=3239 YBD=3241 YBD=3245 YBD=3240 YHG=3215>neutralised YHG=3216>by YHG=3215>various YHG=3215>antitoxins. YHG=3215>The YHG=3215>evidence YHG=3215>in YHG=3216>the YHG=3222>papers YHG=3213>of " XBD=2532 XBD=172 XHG=690 XBD=304 XBD=524 XBD=584 XBD=668 XBD=991 XBD=1090 XBD=1187 XBD=1303 XHG=104 XHG=192 XHG=323 XHG=544 XHG=602 XHG=1038 XHG=1109 XHG=1206 YBD=3246 YBD=3239 YBD=3238 YBD=3245 YBD=3238 YBD=3244 YHG=3209>the YHG=3210>rapid YHG=3210>extension YHG=3209>of YHG=3208>the YHG=3208>gas-gangrene YHG=3209>At YHG=3218>any YHG=3208>rate, ? XBD=1559 XBD=1649 XBD=1837 XHG=1365 XHG=1584 XHG=1676 YBD=3270 YBD=3268 YBD=3273 YHG=3245>Macfarlane YHG=3244>and YHG=3244>Knight,&dquo; |>" " XBD=2529 XBD=2335 XHG=2360 XHG=2109 YBD=3271 YBD=3274 YHG=3245>Macfarlane, YHG=3244>Heyningen,&dquo;2 " XBD=138 XBD=280 XBD=416 XBD=527 XBD=811 XBD=870 XBD=956 XBD=1117 XBD=1304 XHG=104 XHG=160 XHG=300 XHG=436 XHG=548 XHG=833 XHG=890 XHG=978 XHG=1137 YBD=3286 YBD=3278 YBD=3276 YBD=3277 YBD=3277 YBD=3277 YHG=3248>it YHG=3257>seems YHG=3249>likely YHG=3249>that YHG=3247>examination YHG=3247>of YHG=3247>the YHG=3248>wound YHG=3247>exudate " XBD=2530 XBD=1489 XHG=2413 XBD=1575 XBD=1801 XBD=1893 XBD=2045 XBD=2129 XBD=2335 XHG=1366 YBD=3302 XHG=1511 XHG=1596 XHG=1829 XHG=1924 XHG=2065 XHG=2153 XHG=2360 YBD=3300 YHG=3274>however YBD=3297 YBD=3298 YHG=3273>Oakley 21 YHG=3273>Anderson,12 YHG=3263>Oakley YHG=3274>and YHG=3273>Warrack YHG=3275>is " XBD=2387 XBD=233 XHG=706 XBD=383 XBD=476 XBD=677 XBD=745 XBD=1074 XBD=1191 XBD=1303 XHG=104 XHG=257 XHG=408 XHG=502 XHG=771YBD=3316 XHG=1099 XHG=1218 YBD=3317 YBD=3324 YBD=3322 YBD=3315 YBD=3322 YHG=3287>would YHG=3288>reveal YHG=3288>the YHG=3295>presence YHG=3286>of YHG=3286>hyaluronidase, YHG=3286>thus YHG=3295>sug" XBD=1455 XBD=1672 XBD=1774 XBD=1992 XBD=2222 XBD=2335 XBD=2391 XBD=2447 XBD=2529 XHG=1365 XHG=1477 XHG=1708 XHG=1798 XHG=2176 XHG=2240 XHG=2357 XHG=2411 XHG=2465 YBD=3331 YBD=3330 YBD=3326 YBD=3329 YBD=3327 YHG=3302>quite YHG=3302>convincing. YHG=3292>The YHG=3302>conclusions YHG=3302>by YHG=3302>Weed YHG=3303>et YHG=3297>al. YHG=3308>seem " XBD=2529 XBD=1397 XBD=1452 XBD=1538 XBD=1586 XBD=1673 XBD=1720 XBD=1823 XBD=1871 XBD=1969 XBD=2092 XBD=2140 XBD=2244 XBD=2307 XBD=2371 XHG=1361 XHG=1412 XHG=1466 XHG=1552 XHG=1599 XHG=1685 XHG=1732 XHG=1837 XHG=1885 XHG=1980 XHG=2105 XHG=2154 XHG=2256 XHG=2318 XHG=2383 YBD=3358 YBD=3360 YBD=3354 YBD=3355 YBD=3356 YBD=3359 YHG=3332>to YHG=3332>be YHG=3332>due, YHG=3331>in YHG=3331>part YHG=3331>at YHG=3330>least YHG=3330>their YHG=3331>failure YHG=3331>to YHG=3331>allow YHG=3331>for YHG=3331>the YHG=3332>activating XBD=254 XHG=733 XBD=319 XBD=527 XBD=615 XBD=718 XBD=960 XBD=1165 XBD=1305 XHG=105 XHG=267 XHG=331 XHG=544 XHG=628 XHG=974YBD=3354 XHG=1177 YBD=3361 YBD=3355 YBD=3359 YHG=3325>gesting YHG=3334>an YHG=3326>infection, YHG=3326>but YHG=3325>that YHG=3324>differential YHG=3324>diagnosis YHG=3324>would

Boyland, E. and McClean, D. (1935) J. Path. Bact. 41, 553: Duran-Reynals, F. (1933) J. exp. Med. 58, 161. (1939) Ibid, 69, 69. and Stewart, F. W. (1931) Amer. J. Cancer, 15, 2790. Chain, E. and Duthie, E. S. (1940) Brit. J. exp. Path. 21, 324. Claude, A. (1937) J. exp. Med. 66, 353. Crook, E. M. (1942) Brit. J. exp. Path. 23, 37. Hobby, G. L., Dawson, M. H., Meyer, K. and Chaffee, E. (1941) J. exp. Med. 73, 109. Hoffmann, D. C. and Duran-Reynals, F. (1931) Ibid, 53, 387. Macfarlane, M. G. (1942) Biochem. J. 36, proceedings III. and Knight, B. C. J. G. (1941) Ibid, 35, 884. Macfarlane, R. G., Oakley, C. L. and Anderson, C. G. (1941)

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Carter 23 welchii

Crookvan reached

EARLY DIAGNOSIS OF WOUND INFECTION

EARLY DIAGNOSIS OF WOUND INFECTION

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