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Early diagnostics and an approach to treatment MS
EARLY DIAGNOSTICS TREATMENT MS
AND
AN
APPROACH
MEASUREMENTOFIL-12,TNF-aANDTGF-PINSERAANDCSF
TO
OF MS PATIENTS
Dovh&kc&i.,Kuzmenko V.*. lnstttute of Physics, Ac.Sc. Biophysical Division,Kiev, *-Medical and Technical Center ” Medical ecology”,
DrulovicJ.,
Ukraine, Ktev. Ukraine
lnstltute
In the report we would suggest a new approaches to diagnostics and treatment of MS early stages. Diagnostic method is based on the immunological abnormal/ty found in the MS-volnurable people [I] and our previous investigations [2). We propose usmg infrared (IR) spectroscopy of T-and B-lymphocytes and total lymphocyte fraction. IR spectroscopy IS successfully applied for biochemical study of complex biological molecules and cells, identification of viruses and bacterium strains and sometimes for diagnostic purposes in medicine. IR spectroscopy of elements of biological cells and tissues. taken from human body, gives knowledges about structure changes in organism as result of homeostas disfunction. Total and differential fraction of T-and B-lymphocytes are selected from venous blood by special methods. Purity of T-and Bfraction consist In about 90%. The blood are taken from healthy donors and MS patient before and after cause of treatment. The samples are prepared from fresh blood and casted on trasparent in IR range substrates (KRS-S,Si) IR bands in the range of valent and deformatlonal vibrations of CH.CO,NH,PO groups (400-4OOOOcm -’ ) are registered. The most drastic changes are expected to be found in the 2800-3000cm-’ range (corresponding to CH vibrations). The founded correlations may be used as diagnostic criterion As treetment method we used millimeter resonance therapy with tradItIonal medicine. Millimeter resonance therapy is method which uses weak electromagnetic fields of millimeter range, applied to the biological active zones or points of accupunctures of a human body. A number of cases of MS under the method gives symptomatic positive results. I. W. Ritchie Russell, Multiple Sclerosis. 1976,Pergamon Press. 2. Dovbeshko G.l.,Litviniv G.S.,Gridina N.Ya. Application of T-and Blymphocytes infrared spectroscopy for diagnostic purposes,m V Int.Conf. spectr. btol. molecules,271 -272.1993, Kluwer Academic Publishers.
EPIDEMIOLOGY FOREST-STEPPE
E. DubenKo. UnlversltY,
0F MULTIPLE SCLEROSIS ZONE OF UKRAINE
7 Dzhepa: KharkOV,
KharKov UKralne.
IN
Stat0
NORTH
Mostarica-StojkoviC M., StojsavljcviC SokiC D., campie A., Levi6 Z.
of Neurology,
Centre
of Serbia,
Belgrade.
V.,
Yugoslavia
There IS considerable evidence implicatmg the involvement of cytokines in the immunopathogenesis of multiple sclerosis (MS). In OUT study, we assayed mterleukin-12 (IL-12). proinflammatory cytokine tumor nccros~s factor-u (TNF-(I) and immune response-downregulating cytokine transforming growth factor-p
(TGF-P)
in cerehrospinal
fluid
(CSF)
and serum of MS patients. IL-12, a recently of interferon-gama and TNF-a, effective
discovered cytokine, is a potent stimulant initiator of Thl dependent cell-mediated key regulators in EAE. To our knowledge.
immunity and consequently one of the this is the first report on IL-12 in MS.
All 25 patients had definite MS by Poser’s criteria (1983). Ten patients were in acute relapse, 10 were in remission. and 5 patients had chronic progressive MS. Assessment of disability was made in all patients using the Kurtzke Expanded Disability Score (1983). Control samples were obtained from 15 patients with other non-inflammatory neurological diseases (ONND). Concentrations of cytokines in CSF and sera were measured using commercial ELBA kits for K-12. TNF-a and TGF-!31 from R&D Systems, Minneapolis, Minn., USA. For statistical analysis Mann-Whitney test, Student’s t test, Fisher’s test and chi-square analysis were used. In MS patients, 36%. 16% and 0% had elevated serum levels of IL-12, TNF-a and TGF-P, respectively. In CSF, IL-12 was detected in 32% of MS patients, TNF-a in 36% and TGF-P in 37% of MS cases. All three cytokines were also detected in CSF of pahents with ONND. Analysis of IL-l& TNF-a and TGF-P concentrations in CSF and scra revealed no significant differences between MS patients and controls. There was no correlation between the concentration of any cytokine and clinical parameters of disease activity, disability and disease duration. TNF-a was detected m the CSF of 8 MS patients with clinically active disease and only one in remission, hut this difference did not reach statistical significance. According to our results. measurement of IL-12, TNF-a and TGF-fi in CSF and sera cannot he used for monitoring disease activity m majority of MS pattents.
Title -A FOLLOW-UP STUDY OF MS PATIENTS BITIS RETINAE OF SHEATING AROUND RETINAE 15 YEARS AFTER THE INITIAL
OF
WITH PERIPHLEVEINS IN THE DIAGNOSIS.
Medl~al @ME
OF AilTHOR
Tine Engell, MD, Hospital, Denmark.
&:i.amiiiat ion way ivrf9rrtleil WI th muitlple sclerosis
Clinical
N., Pravica
ori 46 3 Patlent
Eye
Department,
Ssnderborg
Abstract Perlphlebltls retlnae(PS) 1s seen ophthalmoscoplcally as white cuffs around veins in retina. It 1.5 asymptomatlc, peripherally located and therefore seen only through dilated pupils. The frequency and duration of PR Indicates that It may be a central symptom In MS (Engell, 1982) PR may drsappear completely or leave residual sheathlng. The present study 1s a follow-up study of 26 earlier published cases (Engell 1986) reexamined after 15 years. None had PR at the time of reexamlnatlon. 9 patients had died, 6 patients showed no changes after PR, 11 patients had resldual sheathing. The patients had en ocular examlnatlon including Goldmanns three mirror contact lens. 12 unpublished cases of retinal sheathlng were also reexamined. No cases of PR were found. All had some degree of sheathlng. A follow-up study of this length has not been performed. Pathoanatomlcally PR is cuffs of lymphocytes and plasmacells slmllar to changes found In the central nervous system in Ms. PR may be part of a dlssemlnated lmmunoloylcal reactlon In the ENS leadlny to plaques formatlon, which 1s not possible In retina due to the lack of myelln at this site.
Who reside In the north elf IJKralne. where there are a lot Of forests (UP to 211 of the terrltorY) and mclist climate prevaie3. Disease lncldence ln that reslon ranged from 21 tO 39 per 100 000. In forest and swamp areas there were more cases (31-35 per 100 000) than in steppe zones (z3-27 Per 100 000). A trend to increasing lncldence among residents who live near a large river (36 Per 100 000) was found. 61% of the Patients reslded in rural regions where the r1SK of SUP@rCOollnB was much higher. No sl8niflcant difference between the disease incidence In males and females was found. 16% Of the Patients fell 111 before 20. 51% before 30. The PrOBreSSlVe tYPe Of the disease was found ln 46. 5% of the Patients, the remittent-progressive one was in 21.27. Thus, tilp investlBated region is characterized by ?li(:?PaSed rlsK factors of multiple sclerosis in Iccmparlson with Other reelons csf UKraine.
-33-
AND
AN
APPROACH
MEASUREMENTOFIL-12,TNF-aANDTGF-PINSERAANDCSF
TO
OF MS PATIENTS
Dovh&kc&i.,Kuzmenko V.*. lnstttute of Physics, Ac.Sc. Biophysical Division,Kiev, *-Medical and Technical Center ” Medical ecology”,
DrulovicJ.,
Ukraine, Ktev. Ukraine
lnstltute
In the report we would suggest a new approaches to diagnostics and treatment of MS early stages. Diagnostic method is based on the immunological abnormal/ty found in the MS-volnurable people [I] and our previous investigations [2). We propose usmg infrared (IR) spectroscopy of T-and B-lymphocytes and total lymphocyte fraction. IR spectroscopy IS successfully applied for biochemical study of complex biological molecules and cells, identification of viruses and bacterium strains and sometimes for diagnostic purposes in medicine. IR spectroscopy of elements of biological cells and tissues. taken from human body, gives knowledges about structure changes in organism as result of homeostas disfunction. Total and differential fraction of T-and B-lymphocytes are selected from venous blood by special methods. Purity of T-and Bfraction consist In about 90%. The blood are taken from healthy donors and MS patient before and after cause of treatment. The samples are prepared from fresh blood and casted on trasparent in IR range substrates (KRS-S,Si) IR bands in the range of valent and deformatlonal vibrations of CH.CO,NH,PO groups (400-4OOOOcm -’ ) are registered. The most drastic changes are expected to be found in the 2800-3000cm-’ range (corresponding to CH vibrations). The founded correlations may be used as diagnostic criterion As treetment method we used millimeter resonance therapy with tradItIonal medicine. Millimeter resonance therapy is method which uses weak electromagnetic fields of millimeter range, applied to the biological active zones or points of accupunctures of a human body. A number of cases of MS under the method gives symptomatic positive results. I. W. Ritchie Russell, Multiple Sclerosis. 1976,Pergamon Press. 2. Dovbeshko G.l.,Litviniv G.S.,Gridina N.Ya. Application of T-and Blymphocytes infrared spectroscopy for diagnostic purposes,m V Int.Conf. spectr. btol. molecules,271 -272.1993, Kluwer Academic Publishers.
EPIDEMIOLOGY FOREST-STEPPE
E. DubenKo. UnlversltY,
0F MULTIPLE SCLEROSIS ZONE OF UKRAINE
7 Dzhepa: KharkOV,
KharKov UKralne.
IN
Stat0
NORTH
Mostarica-StojkoviC M., StojsavljcviC SokiC D., campie A., Levi6 Z.
of Neurology,
Centre
of Serbia,
Belgrade.
V.,
Yugoslavia
There IS considerable evidence implicatmg the involvement of cytokines in the immunopathogenesis of multiple sclerosis (MS). In OUT study, we assayed mterleukin-12 (IL-12). proinflammatory cytokine tumor nccros~s factor-u (TNF-(I) and immune response-downregulating cytokine transforming growth factor-p
(TGF-P)
in cerehrospinal
fluid
(CSF)
and serum of MS patients. IL-12, a recently of interferon-gama and TNF-a, effective
discovered cytokine, is a potent stimulant initiator of Thl dependent cell-mediated key regulators in EAE. To our knowledge.
immunity and consequently one of the this is the first report on IL-12 in MS.
All 25 patients had definite MS by Poser’s criteria (1983). Ten patients were in acute relapse, 10 were in remission. and 5 patients had chronic progressive MS. Assessment of disability was made in all patients using the Kurtzke Expanded Disability Score (1983). Control samples were obtained from 15 patients with other non-inflammatory neurological diseases (ONND). Concentrations of cytokines in CSF and sera were measured using commercial ELBA kits for K-12. TNF-a and TGF-!31 from R&D Systems, Minneapolis, Minn., USA. For statistical analysis Mann-Whitney test, Student’s t test, Fisher’s test and chi-square analysis were used. In MS patients, 36%. 16% and 0% had elevated serum levels of IL-12, TNF-a and TGF-P, respectively. In CSF, IL-12 was detected in 32% of MS patients, TNF-a in 36% and TGF-P in 37% of MS cases. All three cytokines were also detected in CSF of pahents with ONND. Analysis of IL-l& TNF-a and TGF-P concentrations in CSF and scra revealed no significant differences between MS patients and controls. There was no correlation between the concentration of any cytokine and clinical parameters of disease activity, disability and disease duration. TNF-a was detected m the CSF of 8 MS patients with clinically active disease and only one in remission, hut this difference did not reach statistical significance. According to our results. measurement of IL-12, TNF-a and TGF-fi in CSF and sera cannot he used for monitoring disease activity m majority of MS pattents.
Title -A FOLLOW-UP STUDY OF MS PATIENTS BITIS RETINAE OF SHEATING AROUND RETINAE 15 YEARS AFTER THE INITIAL
OF
WITH PERIPHLEVEINS IN THE DIAGNOSIS.
Medl~al @ME
OF AilTHOR
Tine Engell, MD, Hospital, Denmark.
&:i.amiiiat ion way ivrf9rrtleil WI th muitlple sclerosis
Clinical
N., Pravica
ori 46 3 Patlent
Eye
Department,
Ssnderborg
Abstract Perlphlebltls retlnae(PS) 1s seen ophthalmoscoplcally as white cuffs around veins in retina. It 1.5 asymptomatlc, peripherally located and therefore seen only through dilated pupils. The frequency and duration of PR Indicates that It may be a central symptom In MS (Engell, 1982) PR may drsappear completely or leave residual sheathlng. The present study 1s a follow-up study of 26 earlier published cases (Engell 1986) reexamined after 15 years. None had PR at the time of reexamlnatlon. 9 patients had died, 6 patients showed no changes after PR, 11 patients had resldual sheathing. The patients had en ocular examlnatlon including Goldmanns three mirror contact lens. 12 unpublished cases of retinal sheathlng were also reexamined. No cases of PR were found. All had some degree of sheathlng. A follow-up study of this length has not been performed. Pathoanatomlcally PR is cuffs of lymphocytes and plasmacells slmllar to changes found In the central nervous system in Ms. PR may be part of a dlssemlnated lmmunoloylcal reactlon In the ENS leadlny to plaques formatlon, which 1s not possible In retina due to the lack of myelln at this site.
Who reside In the north elf IJKralne. where there are a lot Of forests (UP to 211 of the terrltorY) and mclist climate prevaie3. Disease lncldence ln that reslon ranged from 21 tO 39 per 100 000. In forest and swamp areas there were more cases (31-35 per 100 000) than in steppe zones (z3-27 Per 100 000). A trend to increasing lncldence among residents who live near a large river (36 Per 100 000) was found. 61% of the Patients reslded in rural regions where the r1SK of SUP@rCOollnB was much higher. No sl8niflcant difference between the disease incidence In males and females was found. 16% Of the Patients fell 111 before 20. 51% before 30. The PrOBreSSlVe tYPe Of the disease was found ln 46. 5% of the Patients, the remittent-progressive one was in 21.27. Thus, tilp investlBated region is characterized by ?li(:?PaSed rlsK factors of multiple sclerosis in Iccmparlson with Other reelons csf UKraine.
-33-