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Effect of acute peripheral IL-1β administration on BDNF mRNA expression in the rat brain structures
32
Abstracts – XIX Congress PTF / Pharmacological Reports 67S (2015) 2–45
observed in PSG (1 and 7 days) and MAMG (1, 7, 14 and 21 days). In turn both PSG and MAMG showed the increase in locomotor activity. Biochemical studies indicate that CORT level was increase in PSG and MAMG (evidence of properly performed animal model of schizophrenia). Moreover decrease of BDNF level in the HIP and PFC was observed in PSG and MAMG which correlate with impaired spatial memory and locomotor activity observed in rats. In both animal models of schizophrenia behavioral and biochemical changes in the examined offspring were observed. As the HIP is structure which participate in spatial memory process, the decrease in BDNF observed in the study could be related to spatial memory deterioration found in PSG and MAMG. Moreover decrease level of BDNF observed in PFC could correlate with increase locomotor activity of rats. Animal models of schizophrenia seem to be an important tool to understand the key theories related to the disorder and can be used to assess efficacy of new drugs as well as create a new directions of research. http://dx.doi.org/10.1016/j.pharep.2015.06.096 Effect of acute peripheral IL-1b administration on BDNF mRNA expression in the rat brain structures Ewa Obuchowicz 1,*, Marta M. Nowacka 1,2, Monika Paul-Samojedny 3, Anna M. Bielecka 1 1 Department of Pharmacology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland 2 Laboratory of Molecular Biology, Faculty of Physiotherapy, The Jerzy Kukuczka Academy of Physical Education, Katowice, Poland 3 Department of Medical Genetics, Faculty of Pharmacy in Sosnowiec, Medical University of Silesia, Katowice, Poland *Corresponding author.
Various medical conditions are associated with an activation of peripheral cell-mediated immune, inflammatory, oxidative and nitrosative stress pathways characterized, among the others, by an enhanced levels of pro-inflammatory cytokines [Maes et al, Neuroendocrinol Lett, 2011]. The present study was designed to assess the influence of an increased IL-1b levels at the periphery on brain derived neurotrophic factor (BDNF) mRNA expression in female and male rats and BDNF mRNA in females exposed to chronic social instability stress (CSIS) for 29–30 days. CSIS paradigm is known to be more stressful for females than males. Male rats or female rats in the estrus phase were given an ip injection of recombinant rat IL-1b (50 mg/kg; R&D System) or sterile, non-pyrogenic 0.9% saline (2 ml/kg) 4 h before decapitation. The BDNF mRNA was determined by the qRT-PCR in the pituitary, hypothalamus, hippocampus and amygdala. In females, a tendency towards a reduction in the expression of BDNF mRNA especially in the pituitary gland and hypothalamus was observed. In male rats, IL-1b induced the stronger effect on the studied growth factor in the limbic structures, particularly in the amygdala. In females previously exposed to CSIS procedure, IL1b significantly decreased the number of BDNF mRNA copies as compared to its effect in the non-stressed rats. The results indicate the differences between female and male rats in the response of BDNF to the enhanced peripheral level of IL-1b and suggest that especially under stress condition the peripheral IL-1b overexpression may influence the CNS function. http://dx.doi.org/10.1016/j.pharep.2015.06.097
Anxiolytic-like properties of asenapine and lurasidone in the rat models of anxiety Anna Partyka 1,*, Anna Wasik 1, Małgorzata Piejko 1, Marcin Kołaczkowski 2, Anna Wesołowska 1 1 Department of Clinical Pharmacy, Jagiellonian University Medical College, Krako´w, Poland 2 Department of Medicinal Chemistry, Jagiellonian University Medical College, Krako´w, Poland *Corresponding author.
Common accompaniments of schizophrenia condition are depression and anxiety [Buckley, Schizophr Bull, 2009]. The second-generation of antipsychotics seem to be a new option for the treatment of depressive/anxiety symptoms in schizophrenia. Lurasidone and asenapine are the antipsychotic drugs which have been recently introduced to the market [Ishibashi, J Pharmacol Exp Ther, 2010; Schatzberg, Nemeroff, Textbook of Psychopharmacology, 2009]. Their antidepressant-like activity in the rat forced swim test has been reported recently [Kołaczkowski, Naunyn Schmiedebergs Arch Pharmacol, 2014]. In the present study we examined their anxiolytic-like properties in two wellestablish anxiety rat models: elevated plus maze (EPM) and Vogel conflict drinking test. Asenapine (0.1–1 mg/kg) at a dose of 1 mg/kg significantly increased the percentage of time spent in the open arms, but simultaneously at doses of 0.3 and 1 mg/kg decreased the number of entries into the open arms without affecting the percentage of the open arms entries. Lurasidone did not change significantly the parameters measured in the EPM. In the Vogel test asenapina administered at a dose of 1 mg/kg and lurasidone at a dose of 10 mg/kg substantially increased the number of shocks accepted by rats and the number of licks. Screening of potential antipsychotics for anxiolytic-like properties is considered to be an important part of modern drug development. Our data suggest that asenapina and lurasidone revealed anxiolytic-like effects in EPM and/or in Vogel conflict drinking test in rats. Supported by Funds for Statutory Activity of Jagiellonian University Medical College, Krako´w, Poland (K/ZDS/004121). http://dx.doi.org/10.1016/j.pharep.2015.06.098 Mesoporous silica nanoflakes – Biocompatibility, cellular uptake and drug transport Magdalena Peruz˙ynska 1,*, Mariola Zbroja 1, Klaudia We˛grzynowska 1, Klaudia Trzeciak 1, Sylwia Szela˛g 1, Dorota Rogin´ska 2, Bogusław Machalin´ski 2, Krzysztof Cendrowski 3, Mateusz Kurzawski 1, Ewa Mijowska 3, Marek Droz´dzik 1 1
Department of Experimental and Clinical Pharmacology, Szczecin, Poland 2 Department of General Pathology, Pomeranian Medical University, Szczecin, Poland 3 Institute of Chemical and Environment Engineering, West Pomeranian University of Technology, Szczecin, Poland *Corresponding author. Mesoporous silica nanoflakes are new promising silica nanomaterials. The studied materials were synthesized on graphene sheets core, that after silica coverage was burned out. In
Abstracts – XIX Congress PTF / Pharmacological Reports 67S (2015) 2–45
observed in PSG (1 and 7 days) and MAMG (1, 7, 14 and 21 days). In turn both PSG and MAMG showed the increase in locomotor activity. Biochemical studies indicate that CORT level was increase in PSG and MAMG (evidence of properly performed animal model of schizophrenia). Moreover decrease of BDNF level in the HIP and PFC was observed in PSG and MAMG which correlate with impaired spatial memory and locomotor activity observed in rats. In both animal models of schizophrenia behavioral and biochemical changes in the examined offspring were observed. As the HIP is structure which participate in spatial memory process, the decrease in BDNF observed in the study could be related to spatial memory deterioration found in PSG and MAMG. Moreover decrease level of BDNF observed in PFC could correlate with increase locomotor activity of rats. Animal models of schizophrenia seem to be an important tool to understand the key theories related to the disorder and can be used to assess efficacy of new drugs as well as create a new directions of research. http://dx.doi.org/10.1016/j.pharep.2015.06.096 Effect of acute peripheral IL-1b administration on BDNF mRNA expression in the rat brain structures Ewa Obuchowicz 1,*, Marta M. Nowacka 1,2, Monika Paul-Samojedny 3, Anna M. Bielecka 1 1 Department of Pharmacology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland 2 Laboratory of Molecular Biology, Faculty of Physiotherapy, The Jerzy Kukuczka Academy of Physical Education, Katowice, Poland 3 Department of Medical Genetics, Faculty of Pharmacy in Sosnowiec, Medical University of Silesia, Katowice, Poland *Corresponding author.
Various medical conditions are associated with an activation of peripheral cell-mediated immune, inflammatory, oxidative and nitrosative stress pathways characterized, among the others, by an enhanced levels of pro-inflammatory cytokines [Maes et al, Neuroendocrinol Lett, 2011]. The present study was designed to assess the influence of an increased IL-1b levels at the periphery on brain derived neurotrophic factor (BDNF) mRNA expression in female and male rats and BDNF mRNA in females exposed to chronic social instability stress (CSIS) for 29–30 days. CSIS paradigm is known to be more stressful for females than males. Male rats or female rats in the estrus phase were given an ip injection of recombinant rat IL-1b (50 mg/kg; R&D System) or sterile, non-pyrogenic 0.9% saline (2 ml/kg) 4 h before decapitation. The BDNF mRNA was determined by the qRT-PCR in the pituitary, hypothalamus, hippocampus and amygdala. In females, a tendency towards a reduction in the expression of BDNF mRNA especially in the pituitary gland and hypothalamus was observed. In male rats, IL-1b induced the stronger effect on the studied growth factor in the limbic structures, particularly in the amygdala. In females previously exposed to CSIS procedure, IL1b significantly decreased the number of BDNF mRNA copies as compared to its effect in the non-stressed rats. The results indicate the differences between female and male rats in the response of BDNF to the enhanced peripheral level of IL-1b and suggest that especially under stress condition the peripheral IL-1b overexpression may influence the CNS function. http://dx.doi.org/10.1016/j.pharep.2015.06.097
Anxiolytic-like properties of asenapine and lurasidone in the rat models of anxiety Anna Partyka 1,*, Anna Wasik 1, Małgorzata Piejko 1, Marcin Kołaczkowski 2, Anna Wesołowska 1 1 Department of Clinical Pharmacy, Jagiellonian University Medical College, Krako´w, Poland 2 Department of Medicinal Chemistry, Jagiellonian University Medical College, Krako´w, Poland *Corresponding author.
Common accompaniments of schizophrenia condition are depression and anxiety [Buckley, Schizophr Bull, 2009]. The second-generation of antipsychotics seem to be a new option for the treatment of depressive/anxiety symptoms in schizophrenia. Lurasidone and asenapine are the antipsychotic drugs which have been recently introduced to the market [Ishibashi, J Pharmacol Exp Ther, 2010; Schatzberg, Nemeroff, Textbook of Psychopharmacology, 2009]. Their antidepressant-like activity in the rat forced swim test has been reported recently [Kołaczkowski, Naunyn Schmiedebergs Arch Pharmacol, 2014]. In the present study we examined their anxiolytic-like properties in two wellestablish anxiety rat models: elevated plus maze (EPM) and Vogel conflict drinking test. Asenapine (0.1–1 mg/kg) at a dose of 1 mg/kg significantly increased the percentage of time spent in the open arms, but simultaneously at doses of 0.3 and 1 mg/kg decreased the number of entries into the open arms without affecting the percentage of the open arms entries. Lurasidone did not change significantly the parameters measured in the EPM. In the Vogel test asenapina administered at a dose of 1 mg/kg and lurasidone at a dose of 10 mg/kg substantially increased the number of shocks accepted by rats and the number of licks. Screening of potential antipsychotics for anxiolytic-like properties is considered to be an important part of modern drug development. Our data suggest that asenapina and lurasidone revealed anxiolytic-like effects in EPM and/or in Vogel conflict drinking test in rats. Supported by Funds for Statutory Activity of Jagiellonian University Medical College, Krako´w, Poland (K/ZDS/004121). http://dx.doi.org/10.1016/j.pharep.2015.06.098 Mesoporous silica nanoflakes – Biocompatibility, cellular uptake and drug transport Magdalena Peruz˙ynska 1,*, Mariola Zbroja 1, Klaudia We˛grzynowska 1, Klaudia Trzeciak 1, Sylwia Szela˛g 1, Dorota Rogin´ska 2, Bogusław Machalin´ski 2, Krzysztof Cendrowski 3, Mateusz Kurzawski 1, Ewa Mijowska 3, Marek Droz´dzik 1 1
Department of Experimental and Clinical Pharmacology, Szczecin, Poland 2 Department of General Pathology, Pomeranian Medical University, Szczecin, Poland 3 Institute of Chemical and Environment Engineering, West Pomeranian University of Technology, Szczecin, Poland *Corresponding author. Mesoporous silica nanoflakes are new promising silica nanomaterials. The studied materials were synthesized on graphene sheets core, that after silica coverage was burned out. In