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Effect of amino acid (AA) infusion on glomerular filtration rate (GFR)
0.3
PROTEIN CATABOLISM IN SNAM-NEMODIALYSIS WITH DIFFERENT BIOCHEMICAL PROPERTIES. Bergstrom. Departments of Renal Medicine and Huddinge University Hospital, Stockholm, Sweden.
(S-HD): THE EFFECT OF MEMBRANES A. Alvestrand, A. Gutierrez, J. Wahren, J. Clinical Physiology, Karolinska Institute,
The purpose of this study was to evaluate whether blood-membrane protein catabolism in muscle and thus contribute to HD-associated protein depletion in HD patients.
interaction may trigger protein breakdown and
The exchange of free amino acids across the leg was measured in healthy subjects undergoing S-HD (i.e. in vivo passage of blood through a dialyzer without circulation of dialysate) using dialyzers with cuprophane, cellulose acetate or polysulphone membranes. Plasma amino acids were determined in blood from a femoral vein and artery, respectively. Leg blood flow was determined by occusion pletysmography. Subjects, in whom S-HD was performed using a cuprophane-dialyzer (r-1=16), showed an increased net release of amino acids, expressed as the sum of all measured amino acids, from 11 lf15 nmol/min/lOO g tissue in the basal state to 207k29 nmol/min/lOO g tissue at 345 min after start of S-HD (p
0.4
EFFECT OF AMINO ACID (AA) INFUSION ON GLOMERULAR FILTRATION RATE (GFR). H.T. Khawaia (I), J.M. Jackson, G. Venkat Raman, H.A. Lee. Departmentsof Surgery (I) and Renal Medicine, St. Mary's Hospitai, Portsmouth.U.K.
It has been suggested that a protein load increases glomerularperfusion and GFRl whereas others have reported no effect of AA infusion on this renal function2.We have investigatedthis by studying 45 patients undergoingelective abdominal surgery for 6 postoperativedays. They were haemodynamicallystable, had normal plasma urea and creatinine in the preceeding48 hrs, were matched for age, sex, anthropometric measurements,volume of infusate,and operative procedureand were randomised into 3 groups. Group A (n=l5) received 4% dextrose-saline(DS) delivering6 kcal kg-lday-l. Group B (n=15) received isotonic amino acid (AA) delivering0.15~ nitrogen kg-lday-1. Group C (n=lS) received a central venous infusion which delivered the same amount of nitrogen as group B and 22.5 non-proteinkcal (50% fat, 50% glucose) kg-lday-l.The calculated creatinine clearances (mean + sem) were: Days l-2 Days 3-4 Days 5-6 82.1 f.6.35 81.7 + 4.56 65.4 + 5.39 GROUP A 94.5 + 6.95 83.6 + 5.49' 96.7 + 5.30*+a GROUP B 111.3 + 6.08"' 90.5 + 5.98"" 115.4 2 5.29** GROUP C Analysis of variance; Significancegroups B and C vs group A: * p
PROTEIN CATABOLISM IN SNAM-NEMODIALYSIS WITH DIFFERENT BIOCHEMICAL PROPERTIES. Bergstrom. Departments of Renal Medicine and Huddinge University Hospital, Stockholm, Sweden.
(S-HD): THE EFFECT OF MEMBRANES A. Alvestrand, A. Gutierrez, J. Wahren, J. Clinical Physiology, Karolinska Institute,
The purpose of this study was to evaluate whether blood-membrane protein catabolism in muscle and thus contribute to HD-associated protein depletion in HD patients.
interaction may trigger protein breakdown and
The exchange of free amino acids across the leg was measured in healthy subjects undergoing S-HD (i.e. in vivo passage of blood through a dialyzer without circulation of dialysate) using dialyzers with cuprophane, cellulose acetate or polysulphone membranes. Plasma amino acids were determined in blood from a femoral vein and artery, respectively. Leg blood flow was determined by occusion pletysmography. Subjects, in whom S-HD was performed using a cuprophane-dialyzer (r-1=16), showed an increased net release of amino acids, expressed as the sum of all measured amino acids, from 11 lf15 nmol/min/lOO g tissue in the basal state to 207k29 nmol/min/lOO g tissue at 345 min after start of S-HD (p
0.4
EFFECT OF AMINO ACID (AA) INFUSION ON GLOMERULAR FILTRATION RATE (GFR). H.T. Khawaia (I), J.M. Jackson, G. Venkat Raman, H.A. Lee. Departmentsof Surgery (I) and Renal Medicine, St. Mary's Hospitai, Portsmouth.U.K.
It has been suggested that a protein load increases glomerularperfusion and GFRl whereas others have reported no effect of AA infusion on this renal function2.We have investigatedthis by studying 45 patients undergoingelective abdominal surgery for 6 postoperativedays. They were haemodynamicallystable, had normal plasma urea and creatinine in the preceeding48 hrs, were matched for age, sex, anthropometric measurements,volume of infusate,and operative procedureand were randomised into 3 groups. Group A (n=l5) received 4% dextrose-saline(DS) delivering6 kcal kg-lday-l. Group B (n=15) received isotonic amino acid (AA) delivering0.15~ nitrogen kg-lday-1. Group C (n=lS) received a central venous infusion which delivered the same amount of nitrogen as group B and 22.5 non-proteinkcal (50% fat, 50% glucose) kg-lday-l.The calculated creatinine clearances (mean + sem) were: Days l-2 Days 3-4 Days 5-6 82.1 f.6.35 81.7 + 4.56 65.4 + 5.39 GROUP A 94.5 + 6.95 83.6 + 5.49' 96.7 + 5.30*+a GROUP B 111.3 + 6.08"' 90.5 + 5.98"" 115.4 2 5.29** GROUP C Analysis of variance; Significancegroups B and C vs group A: * p