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Effect of colchicine on human ovulation
830
Communications
in
brief
the uteri. In 5 uteri, the uterine opening l”‘rand cl& sistcd l+ith living fetuses in 1 uterus, or absorbed fctllscs in 2 uteri. In 2 mice. ahdominal pregnancies with living fctuscs wrf: found. The placentas in these gcstarions wcrt’ attached in part to the uterus and in part to abdominal structures. (Figs. 1 and 2.1 Experimental abdominal pregnancy may 1~ helpful in studies of trophoblastic growth with relation to the host and the fctLls but indcpendent trom the uterine factory. Improved opc,rative tcchniquc and possibf! trials in other animals may cnhanc.c production of abdominal pregnancy. Ijility of \\,hich has been sholvn in Fig. 1. Mouse placenta. The arrow indicates the area of transition from intrauterine to extrautcrinc attachment of placenta. A hyalinc structure containing giant cells is seen in the area of transition and separates the placenta from its attachment to the extrauterine fatty tissue. (:~100.1
the rate ot the fcasi01tr clspcri-
rill‘nt.
REFERENCES
I, 2.
Bardawil, W. :I., and Toy, B. I,.: Ann. NC!% York Acad. SC. 80: 197, 1959. Hreshchyshyn. M. M., Bogen, B.. and I,oughran, C. H.: AN. .J. ORST. S: G~XEC. 81: .?OL’. 1961.
Effect of colchicine on human ovulation JOHN
Fig. 2. Mouse placenta. The arrows indicate one of the small vascular formations and a Iarge sinus in the placenta at the site of its extrauterine attachment. The peritoneal fatty tissue to which the placenta is attached shows no inflammatory reaction. ( *30. i
scvcnth only, \vcrc of 2 opening One other
to ninth days of gestation. ilt laparotgestational sacculations of one of the uteri incised in a linear fashion for a distance to 3 mm. with careful avoidance of the of the gestational sac. mouse died from peritonitis and ancscapcd. Th(a remaining 11 mice were sacrificed OII the scvcntcenth to the twentieth days of gcstntion and the uteri examined. All unopcratcd and undisturbrd uteri contained
A.
BOARD,
M.D.
Department of Obstetrics and G’~~~~ecoloy)~, Medical Colle2qe of Virginia, Richmond, Vzrginia. This investigation was supported by o grnn! from A. H. Robins Co., Inc., Richmond, Virginia. COLCI~ICINE, a standard medication fol gout, also has the effect of inhibiting cell mitosis.1 When adult male rabbits were given injections of colchicine for 15 weeks, atrophy and various degenerative changes in the testes \vpr(’ noted, with loss of differentiation from spcrmato,qonia to spermatozoa.* Elcvcn regularly menstruating, nonpregnant prisoners at the Virginia State Industrial Farm for Women volunteered to participate in a stud) designed to determine if colchicine, given durin; ,mcl mrnstrual cycle in the usual maintenance dosage rcromnlcndcd for gout, will supprc\s ~~vulation. A11 of the volunteers had a preliminary cndomelrial biopq during the last half of a mcnstrual cycle, and 2 participants were cxcluderl
II(T~IIW
prolifcrntivc
rndomctrirlrn
~vas
found.
Communications
CASE
PRELIMINARY SCREENING
PHASE I
in brief
831
PHASE II
PROLIFERATIVE SECRETORY
I
SXRETORY
SECRETORY
m
PROLIFERATIVE
PKOLIFERATIVE SECRETORY
5 ECRETORY
SECRETORY
SECRETORY
Fig.
I
SECRETOKY
t.ey: 0
Cycle
In @hich
Colchicine
Cycle
In Which
Placebo
1. Endometrial
_
I
SECRETORY K
$+ _
response
SECRETORY
l
SECRETORY SECRETORY
l
SECRETORY
Was Taken War Taken
@
* *
to colchicine
1 SECRETORY
Withdrew From Study Become “Hair Fell Out” While Taking *
Develyped Abdominal Taktng Placebo
or
placebo
The remaining 9 women were given daily a capsule which contained either colchicine 1.2 mg. or a placebo, according to a double-blind crossover study plan. The medication was given from the first day of a menstrual cycle until vaginal bleeding again began, and endometrial biopsies were performed during the last half of each cycle to determine if ovulation had occurred. Results are outlined in Fig. 1. Ovulation did not occttr in 1 woman who was taking colchicine.
0
1
Placebo.
Pain While
administration.
In no instance was placebo administered associated with anovulation, but all “side-effects” occurred during placebo administration. REFERENCES
1.
2.
Goodman, L. S., and Gilman, A.: The Pharmacological Basis of Therapeutics, ed. 2, New York, 1956, The Macmillan Company, pp. 304-307. Barsoum, H.: J. Pharmacol. & Exper. Thcrap. 115: 319, 1955.
Communications
in
brief
the uteri. In 5 uteri, the uterine opening l”‘rand cl& sistcd l+ith living fetuses in 1 uterus, or absorbed fctllscs in 2 uteri. In 2 mice. ahdominal pregnancies with living fctuscs wrf: found. The placentas in these gcstarions wcrt’ attached in part to the uterus and in part to abdominal structures. (Figs. 1 and 2.1 Experimental abdominal pregnancy may 1~ helpful in studies of trophoblastic growth with relation to the host and the fctLls but indcpendent trom the uterine factory. Improved opc,rative tcchniquc and possibf! trials in other animals may cnhanc.c production of abdominal pregnancy. Ijility of \\,hich has been sholvn in Fig. 1. Mouse placenta. The arrow indicates the area of transition from intrauterine to extrautcrinc attachment of placenta. A hyalinc structure containing giant cells is seen in the area of transition and separates the placenta from its attachment to the extrauterine fatty tissue. (:~100.1
the rate ot the fcasi01tr clspcri-
rill‘nt.
REFERENCES
I, 2.
Bardawil, W. :I., and Toy, B. I,.: Ann. NC!% York Acad. SC. 80: 197, 1959. Hreshchyshyn. M. M., Bogen, B.. and I,oughran, C. H.: AN. .J. ORST. S: G~XEC. 81: .?OL’. 1961.
Effect of colchicine on human ovulation JOHN
Fig. 2. Mouse placenta. The arrows indicate one of the small vascular formations and a Iarge sinus in the placenta at the site of its extrauterine attachment. The peritoneal fatty tissue to which the placenta is attached shows no inflammatory reaction. ( *30. i
scvcnth only, \vcrc of 2 opening One other
to ninth days of gestation. ilt laparotgestational sacculations of one of the uteri incised in a linear fashion for a distance to 3 mm. with careful avoidance of the of the gestational sac. mouse died from peritonitis and ancscapcd. Th(a remaining 11 mice were sacrificed OII the scvcntcenth to the twentieth days of gcstntion and the uteri examined. All unopcratcd and undisturbrd uteri contained
A.
BOARD,
M.D.
Department of Obstetrics and G’~~~~ecoloy)~, Medical Colle2qe of Virginia, Richmond, Vzrginia. This investigation was supported by o grnn! from A. H. Robins Co., Inc., Richmond, Virginia. COLCI~ICINE, a standard medication fol gout, also has the effect of inhibiting cell mitosis.1 When adult male rabbits were given injections of colchicine for 15 weeks, atrophy and various degenerative changes in the testes \vpr(’ noted, with loss of differentiation from spcrmato,qonia to spermatozoa.* Elcvcn regularly menstruating, nonpregnant prisoners at the Virginia State Industrial Farm for Women volunteered to participate in a stud) designed to determine if colchicine, given durin; ,mcl mrnstrual cycle in the usual maintenance dosage rcromnlcndcd for gout, will supprc\s ~~vulation. A11 of the volunteers had a preliminary cndomelrial biopq during the last half of a mcnstrual cycle, and 2 participants were cxcluderl
II(T~IIW
prolifcrntivc
rndomctrirlrn
~vas
found.
Communications
CASE
PRELIMINARY SCREENING
PHASE I
in brief
831
PHASE II
PROLIFERATIVE SECRETORY
I
SXRETORY
SECRETORY
m
PROLIFERATIVE
PKOLIFERATIVE SECRETORY
5 ECRETORY
SECRETORY
SECRETORY
Fig.
I
SECRETOKY
t.ey: 0
Cycle
In @hich
Colchicine
Cycle
In Which
Placebo
1. Endometrial
_
I
SECRETORY K
$+ _
response
SECRETORY
l
SECRETORY SECRETORY
l
SECRETORY
Was Taken War Taken
@
* *
to colchicine
1 SECRETORY
Withdrew From Study Become “Hair Fell Out” While Taking *
Develyped Abdominal Taktng Placebo
or
placebo
The remaining 9 women were given daily a capsule which contained either colchicine 1.2 mg. or a placebo, according to a double-blind crossover study plan. The medication was given from the first day of a menstrual cycle until vaginal bleeding again began, and endometrial biopsies were performed during the last half of each cycle to determine if ovulation had occurred. Results are outlined in Fig. 1. Ovulation did not occttr in 1 woman who was taking colchicine.
0
1
Placebo.
Pain While
administration.
In no instance was placebo administered associated with anovulation, but all “side-effects” occurred during placebo administration. REFERENCES
1.
2.
Goodman, L. S., and Gilman, A.: The Pharmacological Basis of Therapeutics, ed. 2, New York, 1956, The Macmillan Company, pp. 304-307. Barsoum, H.: J. Pharmacol. & Exper. Thcrap. 115: 319, 1955.