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Effect of CRF on ethanol-induced gastric lesions in the rat
EFFECT OF CRF ON MUSCLE WOUND HEALING
THE EFFECTS OF LONG TERii HEPARIN APPLICATION ON ACh-INDUCED ISOLATED ILEUM CONTRACTILITY AND STRUCTURE E. Koc’, N. Zaloglu’, E. Konuksever?,
S. A. Polenov, E.T. Wei, N.M. Kruglova, G.M. Nutttillina,
M.V. Lensman, V.A. Zolotarev, D.P. Dvoretsky, G.V Chernjavskaya, N.L. Izvarina. fQrvlo\: hntitute of Physioloa and Pavlov Medical
B. Turan-’
I ,3.Dept of Physiology, Biophysics, Ankara Univ. ;2.Depts of Engineering Science and Biological Science, ME. T U.; TURKEY
Academy, Saint Petersburg, Russia d Cahfornia, Berkeley, USA.
1Jniversity of
The present study was designed to investigate the effects of long term (one and two months), overdoses (1000 and 2000 IU/kg/day) heparin (Liquamine) applications on the isolated ileum contractility and the histopathological alterations in the ileal tissues. The histopathology of tissues was investigated by using light microscopy. Isolated ileum contractility was measured by using the conventional organ bath system with standard and Ca-free Tyrode perfiasion solutions. Ileum preparations were initially contracted with ACh, maximally and these contraction amplitudes were given as % values. The average amplitude of these contractions of all experimental groups were found to be increased significantly w.r.t. control group, in both pe&sion solutions. Under light microscopy, in our preparations applied 1000 IU/kg/day heparin, we have observed edema in ileal mucosa and neutrophil infiltration in villi. In addition, the glandular tissue degeneration was also seen in 2000 m/kg/day group. We can suggest that most probably, the binding of heparin to the receptor on the cell membrane results Ca-release
In anesthetized rats, muscle wound was performed by cutting of the abdominal muscle wall with local infiltration of the muscle wound with human/rat corticotropinreleasing factor (CRF, 100 mcg per wound) or vehicle Within the first postoperative day after the wound was coapted by sutures, histologically the area of muscle injury of control rats was much more than in CRF treated rats. Also, in CRF treated muscle a minimal number of necrotized muscle fibres could be seen, even infiltration by neurotrophiles was observed demonstrating active scavenging and new blood vessels appeared. During the second postoperative day no hemorrhages, increased collagen tissue growth and a lot of new-formed blood vessels and fibroblasts were seen in CRF treated muscle as compared to the control. One may conclude from this study that the process of muscle wound recovery can be accelerated by CRF application (Supported by the CRDF Grant RBI-161)
ENDOTHELIUM DYSFUNCTION-CRITICAL FACTOR OF SYSTEMIC MICROCIRCULATION DISORDERS N. N. Petrishchev, T. D. Vlasov, M. V. Dubina Department of Pathophysiology, St. Petersburg State Pavlov Medical University, Russia
EFFECT OF CRF ON ETHANOL-INDUCED GASTRIC LESIONS IN THE RAT S.A. Polenov, E.T. Wei, L.D. Fedorova, H.M. Kruglova, V A Zolotarev, G.M. NuttfUllina, M.V. Lensman, D.P. Dvoretsky, G.V. Chernjavskaya, N.L. Izvarina.
Pavlov Institute qf Physiology and Pavlov Medical Academy, Saint Petersburg, Russia & CJniversiry of Cirlifornm, Berkeley. (ISA.
Disorders of microcirculation are an important part of pathogenesis of many diseases. We investigated the functional activity of microvessels in rats, specifically permeability, thromboresistance, thrombogenicity, leukocyte/endothelial cell adhesion and circulating endothelial cells during the following pathological processes: postischemic reperfusion, arterial hypertension We found nonspecific systemic and neoplasia. unidirectional changes in the researched parameters of endothelium functional activity. It is possible to conclude three basic mechanisms of system alteration of microcirculation: 1) disorders of central hemodynamics, 2) disturbances of viscosity of blood, 3) structural and functional disorders of endothelium, resulting in increased leukocyte adhesion, thrombosis and increase of microvascular permeability. The mechanisms of systemic disorders of microcirculation are interconnected. Common in all cases is endothelium dysfunction. Accordingly, the significance of this fact may prove the potential benefit of endothelium protectors such as low-molecular-weight heparin.
Neuropeptide corticotropin-releasing factor (CRF) is known to possess anti-inflammatory property. In this study we tested the possible healing effect of CRF on ethanolinduced gastric mucosal damage. Absolute ethanol (lml) administered intragastrically produced severe gastric mucosal damage in all rats tested. Three hours later human/rat CRF (100 mcg in Iml) was administered intragastrically, control rats received vehicle. Animals were sacrificed 6, 12, 24 or 48h after CRF or vehicle. Macroscopically, hemorrhagic lesions occupying 30-50% of oxyntic mucosa were seen in all rats in four control groups. In 50% of CRF treated rats in every group mucosal hemorrhagic lesions were absent, in other 50% of CRF treated rats mucosal lesions were less pronounced both macro- and microscopically as compared to vehicle. Hence, CRF is capable of accelerating the healing of mucosal damage in rats. ethanol-induced gastric (Supported by the CRDF Grant RBI- 16 1)
194
THE EFFECTS OF LONG TERii HEPARIN APPLICATION ON ACh-INDUCED ISOLATED ILEUM CONTRACTILITY AND STRUCTURE E. Koc’, N. Zaloglu’, E. Konuksever?,
S. A. Polenov, E.T. Wei, N.M. Kruglova, G.M. Nutttillina,
M.V. Lensman, V.A. Zolotarev, D.P. Dvoretsky, G.V Chernjavskaya, N.L. Izvarina. fQrvlo\: hntitute of Physioloa and Pavlov Medical
B. Turan-’
I ,3.Dept of Physiology, Biophysics, Ankara Univ. ;2.Depts of Engineering Science and Biological Science, ME. T U.; TURKEY
Academy, Saint Petersburg, Russia d Cahfornia, Berkeley, USA.
1Jniversity of
The present study was designed to investigate the effects of long term (one and two months), overdoses (1000 and 2000 IU/kg/day) heparin (Liquamine) applications on the isolated ileum contractility and the histopathological alterations in the ileal tissues. The histopathology of tissues was investigated by using light microscopy. Isolated ileum contractility was measured by using the conventional organ bath system with standard and Ca-free Tyrode perfiasion solutions. Ileum preparations were initially contracted with ACh, maximally and these contraction amplitudes were given as % values. The average amplitude of these contractions of all experimental groups were found to be increased significantly w.r.t. control group, in both pe&sion solutions. Under light microscopy, in our preparations applied 1000 IU/kg/day heparin, we have observed edema in ileal mucosa and neutrophil infiltration in villi. In addition, the glandular tissue degeneration was also seen in 2000 m/kg/day group. We can suggest that most probably, the binding of heparin to the receptor on the cell membrane results Ca-release
In anesthetized rats, muscle wound was performed by cutting of the abdominal muscle wall with local infiltration of the muscle wound with human/rat corticotropinreleasing factor (CRF, 100 mcg per wound) or vehicle Within the first postoperative day after the wound was coapted by sutures, histologically the area of muscle injury of control rats was much more than in CRF treated rats. Also, in CRF treated muscle a minimal number of necrotized muscle fibres could be seen, even infiltration by neurotrophiles was observed demonstrating active scavenging and new blood vessels appeared. During the second postoperative day no hemorrhages, increased collagen tissue growth and a lot of new-formed blood vessels and fibroblasts were seen in CRF treated muscle as compared to the control. One may conclude from this study that the process of muscle wound recovery can be accelerated by CRF application (Supported by the CRDF Grant RBI-161)
ENDOTHELIUM DYSFUNCTION-CRITICAL FACTOR OF SYSTEMIC MICROCIRCULATION DISORDERS N. N. Petrishchev, T. D. Vlasov, M. V. Dubina Department of Pathophysiology, St. Petersburg State Pavlov Medical University, Russia
EFFECT OF CRF ON ETHANOL-INDUCED GASTRIC LESIONS IN THE RAT S.A. Polenov, E.T. Wei, L.D. Fedorova, H.M. Kruglova, V A Zolotarev, G.M. NuttfUllina, M.V. Lensman, D.P. Dvoretsky, G.V. Chernjavskaya, N.L. Izvarina.
Pavlov Institute qf Physiology and Pavlov Medical Academy, Saint Petersburg, Russia & CJniversiry of Cirlifornm, Berkeley. (ISA.
Disorders of microcirculation are an important part of pathogenesis of many diseases. We investigated the functional activity of microvessels in rats, specifically permeability, thromboresistance, thrombogenicity, leukocyte/endothelial cell adhesion and circulating endothelial cells during the following pathological processes: postischemic reperfusion, arterial hypertension We found nonspecific systemic and neoplasia. unidirectional changes in the researched parameters of endothelium functional activity. It is possible to conclude three basic mechanisms of system alteration of microcirculation: 1) disorders of central hemodynamics, 2) disturbances of viscosity of blood, 3) structural and functional disorders of endothelium, resulting in increased leukocyte adhesion, thrombosis and increase of microvascular permeability. The mechanisms of systemic disorders of microcirculation are interconnected. Common in all cases is endothelium dysfunction. Accordingly, the significance of this fact may prove the potential benefit of endothelium protectors such as low-molecular-weight heparin.
Neuropeptide corticotropin-releasing factor (CRF) is known to possess anti-inflammatory property. In this study we tested the possible healing effect of CRF on ethanolinduced gastric mucosal damage. Absolute ethanol (lml) administered intragastrically produced severe gastric mucosal damage in all rats tested. Three hours later human/rat CRF (100 mcg in Iml) was administered intragastrically, control rats received vehicle. Animals were sacrificed 6, 12, 24 or 48h after CRF or vehicle. Macroscopically, hemorrhagic lesions occupying 30-50% of oxyntic mucosa were seen in all rats in four control groups. In 50% of CRF treated rats in every group mucosal hemorrhagic lesions were absent, in other 50% of CRF treated rats mucosal lesions were less pronounced both macro- and microscopically as compared to vehicle. Hence, CRF is capable of accelerating the healing of mucosal damage in rats. ethanol-induced gastric (Supported by the CRDF Grant RBI- 16 1)
194