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Effect of diet on the ultrastructure cardiomyocytes
S50
Abstracts / Journal of Biotechnology 185S (2014) S37–S125
The toxicity studies of the “renaissance” pharmacological compound
Using biologically active additive to increase resistance stomach of rats in gamma irradiation
Zhannat Bazarbayeva ∗ , Zura Yessimsiitova, Nurzhanat Ablaikhanova, Sabir Nurtazin, Sultan Tuleukhanov, Galia Alpysbayeva
Akerke Seisenbayeva 1,∗ , Zura Yessimsiitova 1 , Zhannat Bazarbayeva 1 , Arailym Beisbekova 2
Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan E-mail address: [email protected] (Z. Bazarbayeva). Iodine-containing pharmacological compound “Renaissance” (RNS) has anti-inflammatory, detoxifying, immune stimulating effect and can be used in the production of therapeutic food additives as well as for the prevention of iodine deficiency. In the experiment studying toxicity of the pharmacological compound albino rats with a mean body weight of 200 g were used. The following drug dosing have been injected intraperitoneally: 25 ml/kg (5 ml) was 20 ml/kg (4 ml + 1 ml of distilled water), 10 ml/kg (2 ml + 3 ml distilled water), 1 ml/kg (0.2 ml + 4.8 ml distilled water). In all tested doses deaths were not observed. After 14 days of experiment the internal organs were examined. After exposure to RNS at 25 ml/kg macroscopic examination of internal organs revealed a general toxic effect noticed through myocardial damage of liver, kidney, brain and stomach. These dosages have irritant effect on the mucous membranes, which leads to the development of gastritis. At a dose of 1 ml/kg for rats RNS had no toxic effect when used once and did not cause histological changes in internal organs. Thus, the “Renaissance” refers to a category of low-toxic substances when administered once only. http://dx.doi.org/10.1016/j.jbiotec.2014.07.167 Effect of diet on the ultrastructure cardiomyocytes Zura Yessimsiitova ∗ , Zhannat Bazarbayeva, Sabir Nurtazin, Nurzhanat Ablayhanova Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan E-mail address: [email protected] (Z. Yessimsiitova). It is known that one of the characteristics of the overwhelming majority power (80%) of the world’s population, is the lack of quality of products and there are still assessed only on functional parameters. Studies were carried out on 30 albino female rats three months of age with an initial body weight of 150–160 g. Object histological and electron microscopic studies were major populations of myocardial cells intact and experimental rats. With high-calorie food, an increase in the number of mitochondria, the appearances of intracellular edema and microcenters myocytolysis that can undoubtedly further affect the contractile function of the myocardium. In the region of the mitochondria with signs of incipient energy depletion and degradation of membranes developed swelling hyaloplasm, destruction and sarcoplasmic reticulum membranes myelination, fragmentation and lysis of myofibrils, the disappearance of glycogen granules. A lowcalorie diet promotes vitaminized cardiomyocytes on experimental rats expressed compensatory-adaptive reactions, energy provided primarily hypertrophy mitochondria. Energy-dense fat diet causes in cardiomyocytes of rats experienced a partial destruction of mitochondria and sarcoplasmic reticulum. Dietary factor plays an important role in the prevention of the body from the most common nowadays diseases of the cardiovascular system. http://dx.doi.org/10.1016/j.jbiotec.2014.07.168
1 Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan 2 Kazakh National Medical University named after S.D. Asfendiyarov, Almaty, Kazakhstan
E-mail address: [email protected] (A. Seisenbayeva). Modern human activity is carried out in conditions of increasing intensity and duration of adverse physical and chemical environmental factors. The material for this work was the stomach of adult rats – male Wistar three months of age, weighing 180–200 grams. Morphological description and photos do with a microscope Zeiss Axiostar plus. Histological examination of the stomach of rats of the first group showed the usual structure is normal. The results of the histological study of the experimental animals, which were subjected to gamma irradiation without the use of biologically active additives, showed a significant increase in the thickness of the gastric mucosa due to swelling. In the surface epithelium showed signs of degeneration, and in some parts of the surface epithelium desquamation pockets. As a result of histological studies have shown that the study of special product “Kausar” has certain immunomodulatory properties. Identified set of adaptogenic properties of the special product “Kausar” leads us to expect that it is similar to other class of radioprotective agents and immunomodulators correctors tissue metabolism, will be effective as a means of increasing radioresistance during irradiation, which opens up new perspective for the application of this preparation. http://dx.doi.org/10.1016/j.jbiotec.2014.07.169 Associated factors to mastitis in cows voluntary milking system Ioan Hutu 1,∗ , Codruta Chis 2 , Camelia Tulcan 3 , Calin Mircu 4 1 Department of Animal Production and Public Veterinary Health, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 2 Department of Exact Science, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 3 Department of Preclinical Science, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 4 Department of Clinical Science, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania
E-mail address: [email protected] (I. Hutu). Background: In voluntary milking system (VMS) the variables are daily registered; the statistical screening can show the association with the somatic cells count (SCC) or with clinical mastitis (CM). Methods: In a dairy farm with 5.5% mastitis prevalence, the retrospective epidemiological study on 40 variables was carried on to identify the possible risk factor of CM. The milk production (Mp), month (Mo), weight variation (Bw), number of rejections per day (Dr), lactation month (Lm), lactose content (Lc), were included in models to find the risk factors associated with CM
Abstracts / Journal of Biotechnology 185S (2014) S37–S125
The toxicity studies of the “renaissance” pharmacological compound
Using biologically active additive to increase resistance stomach of rats in gamma irradiation
Zhannat Bazarbayeva ∗ , Zura Yessimsiitova, Nurzhanat Ablaikhanova, Sabir Nurtazin, Sultan Tuleukhanov, Galia Alpysbayeva
Akerke Seisenbayeva 1,∗ , Zura Yessimsiitova 1 , Zhannat Bazarbayeva 1 , Arailym Beisbekova 2
Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan E-mail address: [email protected] (Z. Bazarbayeva). Iodine-containing pharmacological compound “Renaissance” (RNS) has anti-inflammatory, detoxifying, immune stimulating effect and can be used in the production of therapeutic food additives as well as for the prevention of iodine deficiency. In the experiment studying toxicity of the pharmacological compound albino rats with a mean body weight of 200 g were used. The following drug dosing have been injected intraperitoneally: 25 ml/kg (5 ml) was 20 ml/kg (4 ml + 1 ml of distilled water), 10 ml/kg (2 ml + 3 ml distilled water), 1 ml/kg (0.2 ml + 4.8 ml distilled water). In all tested doses deaths were not observed. After 14 days of experiment the internal organs were examined. After exposure to RNS at 25 ml/kg macroscopic examination of internal organs revealed a general toxic effect noticed through myocardial damage of liver, kidney, brain and stomach. These dosages have irritant effect on the mucous membranes, which leads to the development of gastritis. At a dose of 1 ml/kg for rats RNS had no toxic effect when used once and did not cause histological changes in internal organs. Thus, the “Renaissance” refers to a category of low-toxic substances when administered once only. http://dx.doi.org/10.1016/j.jbiotec.2014.07.167 Effect of diet on the ultrastructure cardiomyocytes Zura Yessimsiitova ∗ , Zhannat Bazarbayeva, Sabir Nurtazin, Nurzhanat Ablayhanova Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan E-mail address: [email protected] (Z. Yessimsiitova). It is known that one of the characteristics of the overwhelming majority power (80%) of the world’s population, is the lack of quality of products and there are still assessed only on functional parameters. Studies were carried out on 30 albino female rats three months of age with an initial body weight of 150–160 g. Object histological and electron microscopic studies were major populations of myocardial cells intact and experimental rats. With high-calorie food, an increase in the number of mitochondria, the appearances of intracellular edema and microcenters myocytolysis that can undoubtedly further affect the contractile function of the myocardium. In the region of the mitochondria with signs of incipient energy depletion and degradation of membranes developed swelling hyaloplasm, destruction and sarcoplasmic reticulum membranes myelination, fragmentation and lysis of myofibrils, the disappearance of glycogen granules. A lowcalorie diet promotes vitaminized cardiomyocytes on experimental rats expressed compensatory-adaptive reactions, energy provided primarily hypertrophy mitochondria. Energy-dense fat diet causes in cardiomyocytes of rats experienced a partial destruction of mitochondria and sarcoplasmic reticulum. Dietary factor plays an important role in the prevention of the body from the most common nowadays diseases of the cardiovascular system. http://dx.doi.org/10.1016/j.jbiotec.2014.07.168
1 Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan 2 Kazakh National Medical University named after S.D. Asfendiyarov, Almaty, Kazakhstan
E-mail address: [email protected] (A. Seisenbayeva). Modern human activity is carried out in conditions of increasing intensity and duration of adverse physical and chemical environmental factors. The material for this work was the stomach of adult rats – male Wistar three months of age, weighing 180–200 grams. Morphological description and photos do with a microscope Zeiss Axiostar plus. Histological examination of the stomach of rats of the first group showed the usual structure is normal. The results of the histological study of the experimental animals, which were subjected to gamma irradiation without the use of biologically active additives, showed a significant increase in the thickness of the gastric mucosa due to swelling. In the surface epithelium showed signs of degeneration, and in some parts of the surface epithelium desquamation pockets. As a result of histological studies have shown that the study of special product “Kausar” has certain immunomodulatory properties. Identified set of adaptogenic properties of the special product “Kausar” leads us to expect that it is similar to other class of radioprotective agents and immunomodulators correctors tissue metabolism, will be effective as a means of increasing radioresistance during irradiation, which opens up new perspective for the application of this preparation. http://dx.doi.org/10.1016/j.jbiotec.2014.07.169 Associated factors to mastitis in cows voluntary milking system Ioan Hutu 1,∗ , Codruta Chis 2 , Camelia Tulcan 3 , Calin Mircu 4 1 Department of Animal Production and Public Veterinary Health, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 2 Department of Exact Science, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 3 Department of Preclinical Science, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 4 Department of Clinical Science, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania
E-mail address: [email protected] (I. Hutu). Background: In voluntary milking system (VMS) the variables are daily registered; the statistical screening can show the association with the somatic cells count (SCC) or with clinical mastitis (CM). Methods: In a dairy farm with 5.5% mastitis prevalence, the retrospective epidemiological study on 40 variables was carried on to identify the possible risk factor of CM. The milk production (Mp), month (Mo), weight variation (Bw), number of rejections per day (Dr), lactation month (Lm), lactose content (Lc), were included in models to find the risk factors associated with CM