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Effect of disodium calcium versenate on iron excretion in man
Research
Society Abstracts
Western
Society
for Clinical
ABSTRACTS OF PAPERS PRESENTED AT THE SEVENTH ANNUAL
Research
MEETING, PORTLAND,
OREGON, JANUARY
29 AND 30, 1954 EFFECT
OF DISODIUM
CALCIUM
VERSENATE
five-day period. No diminution of prothrombin activity was observed. The practicality of the use of this drug to detect abnormal amounts of iron in the body is being investigated.
ON
William G. Figueroa, William S. Adams,‘” Samuel H. Bassett, Leon Rosove and Frances Davis. V. A. Center, and Dept. Medicine, Univ. California Medical Center, Los Angeles, Calif. IRON
EXCRETION
IN MAN.
EFFECT OF CORTISONE ON EXPERIMENTAL
The effect of oral and intravenous administration of disodium calcium versenate (EDTA) on iron excretion was studied in five patients. Two of these patients had primary hemochromatosis, one hypogonadism, one had a gastrectomy for leiomyosarcoma, one was normal. The patients with primary hemochromatosis and the one with hypogonadism were placed on iron balance during the study. The intravenous administration of disodium calcium versenate was found to increase the twenty-four-hour urinary excretion of iron in the patients with primary hemochromatosis from four- to tenfold, depending on dosage. The twenty-four-hour urinary excretion of iron in one of these patients increased from 0.4 mg. to 5.6 mg. when 4 gm. of the drug were administered intravenously. No further increase in urinary output of iron was obtained when the dosage was increased to 8 gm. per day. The patient with hypogonadism also had moderate increase in urinary iron output. The normal subject and the patient with gastrectomy had only a very slight increase in urinary iron excretion, from 0.4 to 0.8 mg. per twenty-four hours. Oral administration of the drug in a dosage of from 5 to 8 gm. per day failed to increase the urinary output of iron. Preliminary data on the patients on balance suggest that oral administration of the drug in this dosage does not materially affect the fecal iron excretion. No toxicity of the drug was noted, with the exception that one of the patients with hemochromatosis developed a slight diarrhea during the last day of study after he had received a total of 40 gm. of the drug intravenously during a * Asterisks JULY,
1954
indicate
HEMO-
Robert S. Evans* and Russell S. Weiser. V. A. Hospital, and the Depts. Medicine and Microbiology, Univ. Washington School of Medicine, Seattle, Wash. LYTIC
ANEMIA.
Cortisone administration diminishes the rate of blood destruction in patients with autoimmune hemolytic anemia. Study of experimental hemolytic anemia in rabbits was undertaken to gain further information on the action of cortisone on the in viuo destruction of red cells by antibodies. An antirabbit red cell serum was produced by injecting washed rabbit red cells into goats. The hyperimmune goat serum was injected intravenously into both cortisonetreated and control rabbits in doses of 0.5 ml., 0.75 ml. and 1 .O ml./kg. body weight. The average percentage drop in hematocrit and the rate of elimination of Cr3’ tagged cells was less in the cortisone-treated animals than in the controls. A greater increase in hypotonic fragility of the red cells in the cortisone-treated group suggested a sparing of abnormal cells from destruction. Sensitization of red cells by goat globulin as demonstrated by the antiglobulin serum technic was equal in amount and duration in the two groups. A ‘
the
hours but sensitization
goat
serum
antiglobulin
antibody
serum
by a
was detected
method
for
five
to
seven days. An unexpected averaging
finding
18 per
cent
the 20 mg. of cortisone istration was
members. 101
of immune
due
to
an
was a fall in hematocrit in the animals
daily
prior
serum. The
increase
in
measured
by Cr3r technic.
on plasma
volume
could
hematocrit
plasma The
receiving
to the admin-
cortisone
be partially
drop
volume
as
effect
reduced
by
Society Abstracts
Western
Society
for Clinical
ABSTRACTS OF PAPERS PRESENTED AT THE SEVENTH ANNUAL
Research
MEETING, PORTLAND,
OREGON, JANUARY
29 AND 30, 1954 EFFECT
OF DISODIUM
CALCIUM
VERSENATE
five-day period. No diminution of prothrombin activity was observed. The practicality of the use of this drug to detect abnormal amounts of iron in the body is being investigated.
ON
William G. Figueroa, William S. Adams,‘” Samuel H. Bassett, Leon Rosove and Frances Davis. V. A. Center, and Dept. Medicine, Univ. California Medical Center, Los Angeles, Calif. IRON
EXCRETION
IN MAN.
EFFECT OF CORTISONE ON EXPERIMENTAL
The effect of oral and intravenous administration of disodium calcium versenate (EDTA) on iron excretion was studied in five patients. Two of these patients had primary hemochromatosis, one hypogonadism, one had a gastrectomy for leiomyosarcoma, one was normal. The patients with primary hemochromatosis and the one with hypogonadism were placed on iron balance during the study. The intravenous administration of disodium calcium versenate was found to increase the twenty-four-hour urinary excretion of iron in the patients with primary hemochromatosis from four- to tenfold, depending on dosage. The twenty-four-hour urinary excretion of iron in one of these patients increased from 0.4 mg. to 5.6 mg. when 4 gm. of the drug were administered intravenously. No further increase in urinary output of iron was obtained when the dosage was increased to 8 gm. per day. The patient with hypogonadism also had moderate increase in urinary iron output. The normal subject and the patient with gastrectomy had only a very slight increase in urinary iron excretion, from 0.4 to 0.8 mg. per twenty-four hours. Oral administration of the drug in a dosage of from 5 to 8 gm. per day failed to increase the urinary output of iron. Preliminary data on the patients on balance suggest that oral administration of the drug in this dosage does not materially affect the fecal iron excretion. No toxicity of the drug was noted, with the exception that one of the patients with hemochromatosis developed a slight diarrhea during the last day of study after he had received a total of 40 gm. of the drug intravenously during a * Asterisks JULY,
1954
indicate
HEMO-
Robert S. Evans* and Russell S. Weiser. V. A. Hospital, and the Depts. Medicine and Microbiology, Univ. Washington School of Medicine, Seattle, Wash. LYTIC
ANEMIA.
Cortisone administration diminishes the rate of blood destruction in patients with autoimmune hemolytic anemia. Study of experimental hemolytic anemia in rabbits was undertaken to gain further information on the action of cortisone on the in viuo destruction of red cells by antibodies. An antirabbit red cell serum was produced by injecting washed rabbit red cells into goats. The hyperimmune goat serum was injected intravenously into both cortisonetreated and control rabbits in doses of 0.5 ml., 0.75 ml. and 1 .O ml./kg. body weight. The average percentage drop in hematocrit and the rate of elimination of Cr3’ tagged cells was less in the cortisone-treated animals than in the controls. A greater increase in hypotonic fragility of the red cells in the cortisone-treated group suggested a sparing of abnormal cells from destruction. Sensitization of red cells by goat globulin as demonstrated by the antiglobulin serum technic was equal in amount and duration in the two groups. A ‘
the
hours but sensitization
goat
serum
antiglobulin
antibody
serum
by a
was detected
method
for
five
to
seven days. An unexpected averaging
finding
18 per
cent
the 20 mg. of cortisone istration was
members. 101
of immune
due
to
an
was a fall in hematocrit in the animals
daily
prior
serum. The
increase
in
measured
by Cr3r technic.
on plasma
volume
could
hematocrit
plasma The
receiving
to the admin-
cortisone
be partially
drop
volume
as
effect
reduced
by