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Effect of dystocia on serum haptoglobin in Awassi ewes
ELSEVIER
EFFECT
OF DYSTOCIA
ON SERUM HAPTOCLOBIN
IN AWA!31
EM3
D. M. Aziz and M. B. Taha Department of Surgery and Obstetrics College of Veterinary Medicine University of Mosul, Mosul, Iraq Received for publication: November 25, 1996 Accepted: April 8, 1997 ABSTRACT Serum haptoglobin concentration was determined In 102 Iraqi Awassl ewes. Blood samples were collected from 82 ewes before the correction of dystocla, 10 ewes with eutocia 2 to 4 h after parturition and 10 nonpregnant ewes during the seasonal anestrus phase. me mean serum haptoglobin concentration was significantly higher (P
0093-691w97/$17.00 PII 80093891x(97p0273-2
Theriogenology
560
April 1995. These ewes were compared with 10 healthy ewes with eutocla and 10 healthy nonpregnant cues which served as controls. The age of ewes ranged between 2 to 5 yr. Blood samples were collected prior to correction of dystocla, 2 to 4 h after parturition in ewes with eutocia, and during the seasonal anestrus phase from nonpregnant ewes. Serum was separated by centrlfugatlon at 900 x g for 5 min. All serum samples were free of hemolysls. Haptoglobin was measured by determining the binding capacity of serum and quantified against standard by the method of Owen et al. (31, using spectrophotometer (PYEUNICAM PU 8600/England) at 470 nm.
hemoglobin a prepared a a wave length of
Standard sera were prepared by the method of Jones and Mould (2). Analysis of variance among means was used for statistical evaluation of the data (7). RESULTS Results of haptoglobln determination for all animals are shown In Table 1. The serum haptoglobln concentrations were significantly higher In the ewes with all types of dystocla than In ewes with eutocla and the nonpregnant ewes. There was no difference between dystocla Induced by various causes (Table 1). Table 1. Comparison of serum haptoglobln concentrations (mean f SD) lh ewes with various types of dystocla before treatment was begun, with eutocla 2 to 4 hours after parturition and In nonpregnant ewes during the seasonal anestrus phase Condition All ewes with dystocla Ewes with rlngwomb Ewes with fetal oversize Abnormal posture Ewes with eutocla Non-pregnant ewes "b
Ewes n a2 32 16 34 10 10
Haptoglobln (g/L) 0.434 0.499 0.396 0.358 0.266 0.197
f * + + + f
0.195a 0.187a 0.262a 0.166a 0.027b 0.015b
Values with different superscripts differ (PCO.01).
There was a positive relationship between serum haptoglobin concentration and the Interval between onset of dystocla and beginning of treatment. A significant (P
561
Theriogenology
section (n = 38) resulting in 0.503 + 0.207 g/L and manually assisted delivery (n - 44) In 0.0383 + 0.151 g/L (mean + SD; P 48
"b
Ewes n 16 25 22 19
Haptoglobln (g/L) 0.360 0.367 0.475 0.565
+_0.145a +_0.153a + 0.208b f 0.245b
Values with different superscripts differ (P
The elevated serum haptoglobin in ewes with dystocia is probably due to damage to the walls of birth canal cl), which occurs to a similar degree regardless of which type of dystocla is present. Our findings are similar to those reported by Scott et al. (4). The degree of tissue damage, however, seems to be related to the haptoglobin concentration, as Indicated by our finding that the duration of dystocia and surgical invasion of the abdominal cavity are associated with elevated haptoglobin. The results of our study suggest that haptoglobin concentrations are indicative of both the presence and degree of tissue damage which occurs during dystocia and its management, and that early intervention in dystocia decreases tissue damage and post partum complications. REFERENCES 1. Eckersal PD, Par-ton H, Conner JG, Nash AS, Watson T, Douglas
2. 3. 4. 5.
TA. The future. In: Blackmore DJ, Eckersall PD, Evans GO, Sommer H, Stonard D, Woodman DD (eds), Animal Clinical Biochemistry. Cambridge: Cambridge University press, 1988;225-226. Jones GE, Mould DL. Adaptation of the guaiacol (peroxidase) test for haptoglobins to a microtitration plate system. Res Vet Sci 1984;37:87-92. Owen JA, Better FC, Hoban J. A simple method for the determination of serum haptoglobins. J Clin Path 1960;13: 163-164. Scott PR, Murray LD, Penny CD. A preliminary study of serum haptoglobin concentration as a prognostic indicator of ovine dystocia cases. Br Vet J 1992;148:341-355. Skinner JG, Brown RALI, Roberts L. Bovine haptoglobin response in clinically defined field conditions. Vet Ret 1991;128:147-14
562
Theriqpm/ogy
6. Skinner JG, Roberts L. Haptoglobinas an lndlcatorof Infection In sheep. Vet Ret 1994;134:33-36. 7. Steel RG, Torrle JH. Principlesand Procedures of Statistic. New York: McGraw Hill, 1980.
EFFECT
OF DYSTOCIA
ON SERUM HAPTOCLOBIN
IN AWA!31
EM3
D. M. Aziz and M. B. Taha Department of Surgery and Obstetrics College of Veterinary Medicine University of Mosul, Mosul, Iraq Received for publication: November 25, 1996 Accepted: April 8, 1997 ABSTRACT Serum haptoglobin concentration was determined In 102 Iraqi Awassl ewes. Blood samples were collected from 82 ewes before the correction of dystocla, 10 ewes with eutocia 2 to 4 h after parturition and 10 nonpregnant ewes during the seasonal anestrus phase. me mean serum haptoglobin concentration was significantly higher (P
0093-691w97/$17.00 PII 80093891x(97p0273-2
Theriogenology
560
April 1995. These ewes were compared with 10 healthy ewes with eutocla and 10 healthy nonpregnant cues which served as controls. The age of ewes ranged between 2 to 5 yr. Blood samples were collected prior to correction of dystocla, 2 to 4 h after parturition in ewes with eutocia, and during the seasonal anestrus phase from nonpregnant ewes. Serum was separated by centrlfugatlon at 900 x g for 5 min. All serum samples were free of hemolysls. Haptoglobin was measured by determining the binding capacity of serum and quantified against standard by the method of Owen et al. (31, using spectrophotometer (PYEUNICAM PU 8600/England) at 470 nm.
hemoglobin a prepared a a wave length of
Standard sera were prepared by the method of Jones and Mould (2). Analysis of variance among means was used for statistical evaluation of the data (7). RESULTS Results of haptoglobln determination for all animals are shown In Table 1. The serum haptoglobln concentrations were significantly higher In the ewes with all types of dystocla than In ewes with eutocla and the nonpregnant ewes. There was no difference between dystocla Induced by various causes (Table 1). Table 1. Comparison of serum haptoglobln concentrations (mean f SD) lh ewes with various types of dystocla before treatment was begun, with eutocla 2 to 4 hours after parturition and In nonpregnant ewes during the seasonal anestrus phase Condition All ewes with dystocla Ewes with rlngwomb Ewes with fetal oversize Abnormal posture Ewes with eutocla Non-pregnant ewes "b
Ewes n a2 32 16 34 10 10
Haptoglobln (g/L) 0.434 0.499 0.396 0.358 0.266 0.197
f * + + + f
0.195a 0.187a 0.262a 0.166a 0.027b 0.015b
Values with different superscripts differ (PCO.01).
There was a positive relationship between serum haptoglobin concentration and the Interval between onset of dystocla and beginning of treatment. A significant (P
561
Theriogenology
section (n = 38) resulting in 0.503 + 0.207 g/L and manually assisted delivery (n - 44) In 0.0383 + 0.151 g/L (mean + SD; P
"b
Ewes n 16 25 22 19
Haptoglobln (g/L) 0.360 0.367 0.475 0.565
+_0.145a +_0.153a + 0.208b f 0.245b
Values with different superscripts differ (P
The elevated serum haptoglobin in ewes with dystocia is probably due to damage to the walls of birth canal cl), which occurs to a similar degree regardless of which type of dystocla is present. Our findings are similar to those reported by Scott et al. (4). The degree of tissue damage, however, seems to be related to the haptoglobin concentration, as Indicated by our finding that the duration of dystocia and surgical invasion of the abdominal cavity are associated with elevated haptoglobin. The results of our study suggest that haptoglobin concentrations are indicative of both the presence and degree of tissue damage which occurs during dystocia and its management, and that early intervention in dystocia decreases tissue damage and post partum complications. REFERENCES 1. Eckersal PD, Par-ton H, Conner JG, Nash AS, Watson T, Douglas
2. 3. 4. 5.
TA. The future. In: Blackmore DJ, Eckersall PD, Evans GO, Sommer H, Stonard D, Woodman DD (eds), Animal Clinical Biochemistry. Cambridge: Cambridge University press, 1988;225-226. Jones GE, Mould DL. Adaptation of the guaiacol (peroxidase) test for haptoglobins to a microtitration plate system. Res Vet Sci 1984;37:87-92. Owen JA, Better FC, Hoban J. A simple method for the determination of serum haptoglobins. J Clin Path 1960;13: 163-164. Scott PR, Murray LD, Penny CD. A preliminary study of serum haptoglobin concentration as a prognostic indicator of ovine dystocia cases. Br Vet J 1992;148:341-355. Skinner JG, Brown RALI, Roberts L. Bovine haptoglobin response in clinically defined field conditions. Vet Ret 1991;128:147-14
562
Theriqpm/ogy
6. Skinner JG, Roberts L. Haptoglobinas an lndlcatorof Infection In sheep. Vet Ret 1994;134:33-36. 7. Steel RG, Torrle JH. Principlesand Procedures of Statistic. New York: McGraw Hill, 1980.