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Effect of electromagnetic field exposure on blood lymphocyte membrane fluidity
EFFECT OF ELECTROMAGNETIC FIELD EXPOSURE ON BLOOD LYMPHOCYTE MEMBRANE FLUIDITY I. A. Goroshmskaya, Yu. N. Bordyushkov, E. I. Gorlo, I.
BIOCHEMICAL CRITERIA OF ECOTOXICANTS CYTOPATHOGENIC EFFECT E.Davletov, A.Goloshchapov, L.Kalimullina, E.Novosyolova, V.Shchepansky Bashkir State University, Ufa, Bashkir Medical University, Ufa and Research Genetic Laboratory, Sterlitamak, Russia
V. Nescubina Research Oncology Institute, Rostov-on-Don, Russia The significance of membrane structures in biological including adaptive effects of electromagnetic fields (Em) is supposed but its mechanism needs overall investigation. The key role of membrane fluidity (MF) in regulation of membrane processes and oxidative stress sensitivity induced us to research the influence of low frequency EMF (100 HZ, 50 mT, during 20 and 40 min) on lymphocyte MF of lipid-protein contact zones and lipid bilayer in blood of rats and relatively healthy persons by means of fluorescent probe pyrene. The increase of h@ was observed under 20 min exposure of rat lymphocytes to EMF. The study of person lymphocytes revealed opposed changes permitting to divide all persons into two groups according their answer to EMF exposure. In the first group MF decreased by 19-23% under 20 min and by 23-29% under 40 min exposure. In the second group the increase of MF was observed amounting to 47-48% under 40 min exposure. The first group differed from the second by statistically higher initial level of MF. As our previous research has found out decreased level of lymphocyte h4F fluidity in cancer patients, EMF caused increase of this index in rats and persons with initially low MF level may contribute to understanding of positive EMF effect in anticancer therapy.
The analysis of markers enzymes activity changes of subcellular organells in pre-school children residing in the chemical centre - the town of Sterlitamak, was carried out in ecotoxicants effect condition. The increased level of free malatedehidrogenase activity in relation to the control (by 2 - 2,5 times), microsomal acethylesterase (by 1,4 times), P-galactosidase (by 3 - 6 times) was detected in the blood of the children living closest to the industrial area. The investigation has shown lipoperoxidation induction in urine (malondialdehyde content was increased by 1,3 - 2,2 times as well). On the whole the changes revealed show membranotropic effect and cytopathogenic action of chemical factors on the organism. The latter support the results of cytogenetic analyses of the adult population: increased chromosome aberations frequency - 4,6 % among the employees of chemical plants (15 people) and 3,9 % (10 people) in the control group which testifies to increased genotoxicity level (top border limit being about 3 %).
SYNTHESIS AND BLOACTIVITY STUDIES OF MANNICH BASES DERIVED FROM ACETOPHENONE Hahse Inci Gull.‘, Jouko Vepslllinen2, Mustafa GuI’.~, TarJa Ojanen’, Osmo HPnninen’ I: Department of Pharmaceutical Chemistry, Ege Universiry, Izmir, 4. Department of Physiology, Adnan Menderes Universiv, Aydin,
DISRUPTIONS IN NEUROENDOCRINE SYSTEM CAUSED BY LONG-TIME INTAKE OF LOW DOSE OF CESIUM-W7 L. Derevyanko Scientijc Center of Radiation Medicine of the Academy of Medical Sciences of Ukraine, Kiev, Ukraine
TURKEY. 2: Department of Chemistry, and 3: Department of Physrology. Giversi@ of Kuopro, 5: Department of Clmical Mtcrobiologv, Kuopio Universify Hospital, Kuopio. FINLAND.
Goal of the study is to investigate the long-time impact of incorporated cesium-137 on hormonal status and ultrastructure of neuroendocrine organs. Experiments were conducted with mature rats which received daily with food Cs-137 in dose of 600 Bq during 1,3,6,9 and 18 months. It was shown that by indices investigated there are some statistically reliable changes in hypothalamus - hypophysis - thyroid, hypothalamus -hypophysis - adrenal and hypothalamus - hypophysis - gonade system. By data of electronic microscopy disruptions have been established for penetration of membranes of endocrine organs, Destructive-dystrophic changes of different degree of pronunciation of parenchyma of organs investigated were observed. They were connected with disruption of intraorgan blood circulation. The most pronounced disruptions of intraorgan blood circulation were observed in brain with prevalent disruption of hypothalamus section, hypophysis, thyroid, gonads.
Mannich bases of acetophenones have shown activit] against various turnours. The objectives of our study were: First, to synthesize some mono-Mannich bases, and to modify them to their corresponding bis-Mannich bases. Quatemary derivatives of selected mono and bis-Mannich bases were also synthesized. Second, to examme their bioactivies. Compounds were synthesized by using dlmethylamine, plperidin and morphohne as an amine component in the Mannich reaction. Bioactivity tests were realised as antimicrobial and cytotoxic activities. AntImIcrobial activities were exammed against some gram negative and positive bacteria and some fungi. Cytotoxicity was tested against renal carcinoma cells of the mouse by using inhibition of colony formation assay. Morphohne contaming mono-Manmch bases and quatemary Mannich bases demonstrated antifungal activity m the concentrahon range of 2-128 pg/ml, wlnle the compounds synthesized did not show antlbactenal activity m this range. According to cytotoxlclty results, ICSO values of compounds were in 0.44-57 FM concentration range. Conversion of mono derlvatlves to bls Increased cytotoxicity 33- 124 times. compounds the Quatemisation procedure Increased antifungal activity, but did not affect the cytotox&. Morpholine containing monoManmch bases and quaternary Mannich bases with antifungal activity and bls-Manmch bases with cytotoxicity seemed to be promlsmg compounds for further drug design.
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BIOCHEMICAL CRITERIA OF ECOTOXICANTS CYTOPATHOGENIC EFFECT E.Davletov, A.Goloshchapov, L.Kalimullina, E.Novosyolova, V.Shchepansky Bashkir State University, Ufa, Bashkir Medical University, Ufa and Research Genetic Laboratory, Sterlitamak, Russia
V. Nescubina Research Oncology Institute, Rostov-on-Don, Russia The significance of membrane structures in biological including adaptive effects of electromagnetic fields (Em) is supposed but its mechanism needs overall investigation. The key role of membrane fluidity (MF) in regulation of membrane processes and oxidative stress sensitivity induced us to research the influence of low frequency EMF (100 HZ, 50 mT, during 20 and 40 min) on lymphocyte MF of lipid-protein contact zones and lipid bilayer in blood of rats and relatively healthy persons by means of fluorescent probe pyrene. The increase of h@ was observed under 20 min exposure of rat lymphocytes to EMF. The study of person lymphocytes revealed opposed changes permitting to divide all persons into two groups according their answer to EMF exposure. In the first group MF decreased by 19-23% under 20 min and by 23-29% under 40 min exposure. In the second group the increase of MF was observed amounting to 47-48% under 40 min exposure. The first group differed from the second by statistically higher initial level of MF. As our previous research has found out decreased level of lymphocyte h4F fluidity in cancer patients, EMF caused increase of this index in rats and persons with initially low MF level may contribute to understanding of positive EMF effect in anticancer therapy.
The analysis of markers enzymes activity changes of subcellular organells in pre-school children residing in the chemical centre - the town of Sterlitamak, was carried out in ecotoxicants effect condition. The increased level of free malatedehidrogenase activity in relation to the control (by 2 - 2,5 times), microsomal acethylesterase (by 1,4 times), P-galactosidase (by 3 - 6 times) was detected in the blood of the children living closest to the industrial area. The investigation has shown lipoperoxidation induction in urine (malondialdehyde content was increased by 1,3 - 2,2 times as well). On the whole the changes revealed show membranotropic effect and cytopathogenic action of chemical factors on the organism. The latter support the results of cytogenetic analyses of the adult population: increased chromosome aberations frequency - 4,6 % among the employees of chemical plants (15 people) and 3,9 % (10 people) in the control group which testifies to increased genotoxicity level (top border limit being about 3 %).
SYNTHESIS AND BLOACTIVITY STUDIES OF MANNICH BASES DERIVED FROM ACETOPHENONE Hahse Inci Gull.‘, Jouko Vepslllinen2, Mustafa GuI’.~, TarJa Ojanen’, Osmo HPnninen’ I: Department of Pharmaceutical Chemistry, Ege Universiry, Izmir, 4. Department of Physiology, Adnan Menderes Universiv, Aydin,
DISRUPTIONS IN NEUROENDOCRINE SYSTEM CAUSED BY LONG-TIME INTAKE OF LOW DOSE OF CESIUM-W7 L. Derevyanko Scientijc Center of Radiation Medicine of the Academy of Medical Sciences of Ukraine, Kiev, Ukraine
TURKEY. 2: Department of Chemistry, and 3: Department of Physrology. Giversi@ of Kuopro, 5: Department of Clmical Mtcrobiologv, Kuopio Universify Hospital, Kuopio. FINLAND.
Goal of the study is to investigate the long-time impact of incorporated cesium-137 on hormonal status and ultrastructure of neuroendocrine organs. Experiments were conducted with mature rats which received daily with food Cs-137 in dose of 600 Bq during 1,3,6,9 and 18 months. It was shown that by indices investigated there are some statistically reliable changes in hypothalamus - hypophysis - thyroid, hypothalamus -hypophysis - adrenal and hypothalamus - hypophysis - gonade system. By data of electronic microscopy disruptions have been established for penetration of membranes of endocrine organs, Destructive-dystrophic changes of different degree of pronunciation of parenchyma of organs investigated were observed. They were connected with disruption of intraorgan blood circulation. The most pronounced disruptions of intraorgan blood circulation were observed in brain with prevalent disruption of hypothalamus section, hypophysis, thyroid, gonads.
Mannich bases of acetophenones have shown activit] against various turnours. The objectives of our study were: First, to synthesize some mono-Mannich bases, and to modify them to their corresponding bis-Mannich bases. Quatemary derivatives of selected mono and bis-Mannich bases were also synthesized. Second, to examme their bioactivies. Compounds were synthesized by using dlmethylamine, plperidin and morphohne as an amine component in the Mannich reaction. Bioactivity tests were realised as antimicrobial and cytotoxic activities. AntImIcrobial activities were exammed against some gram negative and positive bacteria and some fungi. Cytotoxicity was tested against renal carcinoma cells of the mouse by using inhibition of colony formation assay. Morphohne contaming mono-Manmch bases and quatemary Mannich bases demonstrated antifungal activity m the concentrahon range of 2-128 pg/ml, wlnle the compounds synthesized did not show antlbactenal activity m this range. According to cytotoxlclty results, ICSO values of compounds were in 0.44-57 FM concentration range. Conversion of mono derlvatlves to bls Increased cytotoxicity 33- 124 times. compounds the Quatemisation procedure Increased antifungal activity, but did not affect the cytotox&. Morpholine containing monoManmch bases and quaternary Mannich bases with antifungal activity and bls-Manmch bases with cytotoxicity seemed to be promlsmg compounds for further drug design.
142