- Email: [email protected]
Effect of equimolar amounts of long chain triglycerides and medium chain triglycerides on small intestinal transit
P.91 Effect of ornithine a-ketoglutarate polymorphonuclear oxidative metabolism the burned rats
on in
immuno-modulating properties which might be beneficial to burn patients by reducing susceptibility to infection.
M. Roth-Arveiller’, J. Fontagne”-z, C. Coudray-Luca?, J. Le Bouche?, J. P. Giroud’, L. Cynobe? Department of Pharmacology, CNRS URA 595(‘)andAssociation Claude Bernard”), Hbpital Cochin, 27 rue du Fg St Jacques, 75014 PARIS-GRENEMH(3J CHlJ Saint-Antoine, 750 12 PARIS
P.92 Effect of equimolar amounts of long chain triglycerides and medium chain triglycerides on small intestinal transit. M. Ledeboer, A. A. M. Masclee, J. M. B. J. Jansen’, C. B. H. W. Lamers Dept. of Gastroenterology and Hepatology. University Hospitals of Leiden and Nijmegen’, The Netherlands
An impairment of polymorphonuclear (PMN) functions is suspected to contribute greatly to increased susceptibility of burn subjects to infection. Ornithine a-ketoglutarate (OKG) improved nutritional status in burn, septic and polytraumatized patients by increasing protein synthesis and/or reducing protein breakdown. Its chemical structure led us to hypothesize that OKG might, as a source of NO, polyamines and/or glutamine, exert immunomodulating effect on burnimmunodepressed subjects. The aim of this study was to investigate the influence of OKG administered enterally on the immunological status of burned rats, Four groups of rats were considered: 1) healthy rats receiving standard diet of 210 Kcal/kg/day supplemented with 5 g/kg/day of OKG, 2) burned rats receiving the same diet, 3) healthy rats receiving standard diet supplemented with glycine in isonitrogenous amount to OKG, 4) burned rats receiving this diet. Two rats of each group were studied in parallel in each experiment repeated four times. Animals were fed three times a day from day 1 until day 3 when they were sacrificed. Thymus and EDL muscles were taken and weighed and PMN harvested in the pleural cavity. PMN oxidative metabolism was measured by means of 02m generation and chemiluminescent response to opsonized zymosan (OZ). The weights of thymus and EDL muscles from healthy rats were similar. Those of burned control rats demonstrated a significant decrease (p < 0.05) whereas those of rats treated by OKG did not. 02- generation by OZ-stimulated PMN were similar (5+0.5 nmol 0,./min/l O6 cells) for non burned rats of the 1st and 3rd group. On the opposite that of burned control rats (group 4) was significantly (p < 0.05) depressed (4+0.2 nmoiO,-/min/l O6cells) whereas that of OKG treated rats (arouo 2) was not (5.1 +0.2 nmol O,~/min/lO’ cells). These studies’showed that OKG supplementation prevented loss of thymus and EDL muscle weight after burn injury and restored PMN reactivity to OZ. Hence, OKG demonstrated
Medium-chain triglycerides (MCT) are increasingly used as a calorie source for patients with impaired digestion or absorption. The use of MCT in diets is however limited by the frequent occurrence of diarrhoea after MCT ingestion, although MCT, unlike long-chain triglycerides (LCT) is readily,digested and absorbed from the intestinal lumen. Previous studies have suggested that CCK accelerates intestinal transit. However, MCT, in contrast to LCT, does not stimulate CCK secretion. We have investigated the effect of equimolar amounts of LCT (corn oil, 93% Cl 6X18) and MCT (CeresMCT dietary oil, 98% C8-ClO) on CCK secretion and small bowel transit time (SBTT) measured with lactulose H2 breath testing. Lactulose (6 g in 70 ml water) was administered direct into the duodenum instead of orally to exclude the influence of gastric emptying. Transit-time was defined as a sustained increase in breath hydrogen concentration at least 10 ppm above basal values. Ten healthy volunteers (7 men; mean age 25 yr, range: 19-39 yr) were studied during continuous intraduodenal infusion for 120 min of 1) saline (control), 2) MCT 20 mmol/h or 3) LCT 20 mmol/h. Plasma CCK was determined at regular intervals. Results: LCT significantly increased CCK secretion (Amax 1.7kO.3 pmol/l) while no alterations in plasma CCK were observed during MCT or saline administration. Mean SBTT during saline infusion was 77+10 min and did not alter during LCT administration (77& 11 min). lntraduodenal administration of MCT significantly accelerated SBTT (59+6 min, p < 0.05) compared to both LCT and saline. It is concluded that intraduodenal infusion of LCT does not affect small intestinal transit, although significantly increasing CCK secretion, whereas MCT accelerates small intestinal transit, independent of CCK.
64
on in
immuno-modulating properties which might be beneficial to burn patients by reducing susceptibility to infection.
M. Roth-Arveiller’, J. Fontagne”-z, C. Coudray-Luca?, J. Le Bouche?, J. P. Giroud’, L. Cynobe? Department of Pharmacology, CNRS URA 595(‘)andAssociation Claude Bernard”), Hbpital Cochin, 27 rue du Fg St Jacques, 75014 PARIS-GRENEMH(3J CHlJ Saint-Antoine, 750 12 PARIS
P.92 Effect of equimolar amounts of long chain triglycerides and medium chain triglycerides on small intestinal transit. M. Ledeboer, A. A. M. Masclee, J. M. B. J. Jansen’, C. B. H. W. Lamers Dept. of Gastroenterology and Hepatology. University Hospitals of Leiden and Nijmegen’, The Netherlands
An impairment of polymorphonuclear (PMN) functions is suspected to contribute greatly to increased susceptibility of burn subjects to infection. Ornithine a-ketoglutarate (OKG) improved nutritional status in burn, septic and polytraumatized patients by increasing protein synthesis and/or reducing protein breakdown. Its chemical structure led us to hypothesize that OKG might, as a source of NO, polyamines and/or glutamine, exert immunomodulating effect on burnimmunodepressed subjects. The aim of this study was to investigate the influence of OKG administered enterally on the immunological status of burned rats, Four groups of rats were considered: 1) healthy rats receiving standard diet of 210 Kcal/kg/day supplemented with 5 g/kg/day of OKG, 2) burned rats receiving the same diet, 3) healthy rats receiving standard diet supplemented with glycine in isonitrogenous amount to OKG, 4) burned rats receiving this diet. Two rats of each group were studied in parallel in each experiment repeated four times. Animals were fed three times a day from day 1 until day 3 when they were sacrificed. Thymus and EDL muscles were taken and weighed and PMN harvested in the pleural cavity. PMN oxidative metabolism was measured by means of 02m generation and chemiluminescent response to opsonized zymosan (OZ). The weights of thymus and EDL muscles from healthy rats were similar. Those of burned control rats demonstrated a significant decrease (p < 0.05) whereas those of rats treated by OKG did not. 02- generation by OZ-stimulated PMN were similar (5+0.5 nmol 0,./min/l O6 cells) for non burned rats of the 1st and 3rd group. On the opposite that of burned control rats (group 4) was significantly (p < 0.05) depressed (4+0.2 nmoiO,-/min/l O6cells) whereas that of OKG treated rats (arouo 2) was not (5.1 +0.2 nmol O,~/min/lO’ cells). These studies’showed that OKG supplementation prevented loss of thymus and EDL muscle weight after burn injury and restored PMN reactivity to OZ. Hence, OKG demonstrated
Medium-chain triglycerides (MCT) are increasingly used as a calorie source for patients with impaired digestion or absorption. The use of MCT in diets is however limited by the frequent occurrence of diarrhoea after MCT ingestion, although MCT, unlike long-chain triglycerides (LCT) is readily,digested and absorbed from the intestinal lumen. Previous studies have suggested that CCK accelerates intestinal transit. However, MCT, in contrast to LCT, does not stimulate CCK secretion. We have investigated the effect of equimolar amounts of LCT (corn oil, 93% Cl 6X18) and MCT (CeresMCT dietary oil, 98% C8-ClO) on CCK secretion and small bowel transit time (SBTT) measured with lactulose H2 breath testing. Lactulose (6 g in 70 ml water) was administered direct into the duodenum instead of orally to exclude the influence of gastric emptying. Transit-time was defined as a sustained increase in breath hydrogen concentration at least 10 ppm above basal values. Ten healthy volunteers (7 men; mean age 25 yr, range: 19-39 yr) were studied during continuous intraduodenal infusion for 120 min of 1) saline (control), 2) MCT 20 mmol/h or 3) LCT 20 mmol/h. Plasma CCK was determined at regular intervals. Results: LCT significantly increased CCK secretion (Amax 1.7kO.3 pmol/l) while no alterations in plasma CCK were observed during MCT or saline administration. Mean SBTT during saline infusion was 77+10 min and did not alter during LCT administration (77& 11 min). lntraduodenal administration of MCT significantly accelerated SBTT (59+6 min, p < 0.05) compared to both LCT and saline. It is concluded that intraduodenal infusion of LCT does not affect small intestinal transit, although significantly increasing CCK secretion, whereas MCT accelerates small intestinal transit, independent of CCK.
64