- Email: [email protected]
Effect of flaking on some physicochemical and functional properties of wheat and barley
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Abstracts / Journal of Biotechnology 231S (2016) S4–S109
Selective separation of lactic acid after fermentation process using novel polymeric ion-exchange resin Magdalena Legan ∗ , Andrzej Witold Trochimczuk Department of Chemistry, Wroclaw University of Technology, Poland
From idea to working prototype, these activities will engage students in practical, real-world biotechnology challenges and open-ended problem solving. Acknowledgement: This research was supported by “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania, Contract 30891/30.12.2015. http://dx.doi.org/10.1016/j.jbiotec.2016.05.211
E-mail address: [email protected] (M. Legan). Production of lactic acid will be growing rapidly in next decades as it can be used for the synthesis of biodegradable plastic, for the production of biopolymers used in medicine, food and textile industry. Currently, the world production of lactic acid is 80,000 tons per year of which 90% in fermentation processes. Separation process takes a large part of the total cost in lactic acid production. In order to reduce the cost, many studies concerning lactic acid separation have been conducted using different separation techniques such as membrane filtration, ion exchange separation and electrodialysis. Adsorption on ion exchange resin is a practical and economical method often used in industry. The aim of this work was to obtain and characterize the selectivity of a new type of ion-exchanger – porous, highly basic monolith with HIPE (High Internal Phase Emulsion) structure. Hydrophilic monolith of bis-vinylimidazolium salts with HIPE structure was prepared in the water in oil in water (w/o/w) emulsion. Porous structure was checked using SEM. Ionexchange selectivity of lactic acid was tested from multicomponent solutions. Composition of the solutions was: lactic acid, citric acid, acetaldehyde, succinic acid, acetic acid. The concentration of lactic acid before and after the sorption was measured using HPLC. New, hydrophilic monolithic material presented in this work, can be used in separation lactic acid from the fermentation solutions. http://dx.doi.org/10.1016/j.jbiotec.2016.05.210 Using hands-on robotic projects to engage undergraduate students in collaborative problem solving on biotechnology issues Gabriela Gladiola Andruseac Department of Biomedical Science, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania E-mail address: [email protected]. This paper discusses the use of robotic technology to enhance teaching of biotechnology concepts to undergraduate students at the Department of Biomedical Science, Faculty of Medical Bioengineering, Iasi, Romania. Teams of students are challenged to design and build their own robot using hardware elements including: microcontrollers (Arduino, Mindstorm EV3), actuators, sensors (pH, temperature, oxygen) and mechanical parts (gears, wheels). Using a graphical programming environment such as RoboLab they program their robot to perform autonomous tasks: monitoring and real-time Bluetooth transmission of parameters that came from sensors; takes decisions based on information received from the sensors regarding on its future actions. Thus, the robot responds to sensor inputs and use outputs (actuators/sounds/lights) to control the environment. Moreover, in the dynamics of bioanalytical processes the robotic platform allows the student to do a ‘smart’ collection of data: only when this is rapidly changing or when a certain sensor is in a certain field of variation of the monitored parameters. Additionally, the students can manipulate the robot remotely if it is in a potentially dangerous environment or they can demand the robot to act autonomously.
Effect of flaking on some physicochemical and functional properties of wheat and barley Adrian Caprita 1,∗ , Rodica Caprita 2 1
Food Technologies Department, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 2 Department of Biotechnologies, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Romania E-mail address: [email protected] (A. Caprita). Dietary fiber (DF) includes non-starch polysaccharides resistant to digestion in the small intestine and fermentable in the large intestine: arabinoxylan, inulin, pectin, bran, cellulose, -glucan and resistant starch. DF has an important role in improving human health. Consuming adequate quantities of DF have beneficial physiological effects: improving gastrointestinal health, decreasing cholesterolemia and glycemia, and preventing heart disease and cancer. The physiological parameters are influenced by the physicochemical properties, especially by the soluble DF fraction, as this is responsible of the changes in intestinal viscosity. Increased intestinal viscosity delays nutrient absorption, rate of passage and increases bile acid excretion. Food processing, such as flaking, has great effects on DF, especially on the molecular weight distributions. The experiments showed that on flaking, the water binding capacities and swelling capacities remained almost the same. Cereal flakes had much higher aqueous extract viscosity (AEV) than raw cereals, caused by the structural and physicochemical changes. Although total fiber values of the flakes are comparable to those of raw cereals, a redistribution of insoluble to soluble fiber occurred. Flaking resulted in an increased degradation of the molecular mass of DF. This caused a reduction in the content of insoluble DF fraction and an increase in that of the soluble fiber fraction. While increasing the solubility of -glucans and arabinoxylans, the most important water-extractable polysaccharides, an increase in AEV was also observed: 3.48 times in wheat and 4.56 times in barley. The viscosity measurement prior to ingestion can be used to predict intestinal viscosity of processed cereals. http://dx.doi.org/10.1016/j.jbiotec.2016.05.212
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
Selective separation of lactic acid after fermentation process using novel polymeric ion-exchange resin Magdalena Legan ∗ , Andrzej Witold Trochimczuk Department of Chemistry, Wroclaw University of Technology, Poland
From idea to working prototype, these activities will engage students in practical, real-world biotechnology challenges and open-ended problem solving. Acknowledgement: This research was supported by “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania, Contract 30891/30.12.2015. http://dx.doi.org/10.1016/j.jbiotec.2016.05.211
E-mail address: [email protected] (M. Legan). Production of lactic acid will be growing rapidly in next decades as it can be used for the synthesis of biodegradable plastic, for the production of biopolymers used in medicine, food and textile industry. Currently, the world production of lactic acid is 80,000 tons per year of which 90% in fermentation processes. Separation process takes a large part of the total cost in lactic acid production. In order to reduce the cost, many studies concerning lactic acid separation have been conducted using different separation techniques such as membrane filtration, ion exchange separation and electrodialysis. Adsorption on ion exchange resin is a practical and economical method often used in industry. The aim of this work was to obtain and characterize the selectivity of a new type of ion-exchanger – porous, highly basic monolith with HIPE (High Internal Phase Emulsion) structure. Hydrophilic monolith of bis-vinylimidazolium salts with HIPE structure was prepared in the water in oil in water (w/o/w) emulsion. Porous structure was checked using SEM. Ionexchange selectivity of lactic acid was tested from multicomponent solutions. Composition of the solutions was: lactic acid, citric acid, acetaldehyde, succinic acid, acetic acid. The concentration of lactic acid before and after the sorption was measured using HPLC. New, hydrophilic monolithic material presented in this work, can be used in separation lactic acid from the fermentation solutions. http://dx.doi.org/10.1016/j.jbiotec.2016.05.210 Using hands-on robotic projects to engage undergraduate students in collaborative problem solving on biotechnology issues Gabriela Gladiola Andruseac Department of Biomedical Science, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania E-mail address: [email protected]. This paper discusses the use of robotic technology to enhance teaching of biotechnology concepts to undergraduate students at the Department of Biomedical Science, Faculty of Medical Bioengineering, Iasi, Romania. Teams of students are challenged to design and build their own robot using hardware elements including: microcontrollers (Arduino, Mindstorm EV3), actuators, sensors (pH, temperature, oxygen) and mechanical parts (gears, wheels). Using a graphical programming environment such as RoboLab they program their robot to perform autonomous tasks: monitoring and real-time Bluetooth transmission of parameters that came from sensors; takes decisions based on information received from the sensors regarding on its future actions. Thus, the robot responds to sensor inputs and use outputs (actuators/sounds/lights) to control the environment. Moreover, in the dynamics of bioanalytical processes the robotic platform allows the student to do a ‘smart’ collection of data: only when this is rapidly changing or when a certain sensor is in a certain field of variation of the monitored parameters. Additionally, the students can manipulate the robot remotely if it is in a potentially dangerous environment or they can demand the robot to act autonomously.
Effect of flaking on some physicochemical and functional properties of wheat and barley Adrian Caprita 1,∗ , Rodica Caprita 2 1
Food Technologies Department, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania 2 Department of Biotechnologies, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Romania E-mail address: [email protected] (A. Caprita). Dietary fiber (DF) includes non-starch polysaccharides resistant to digestion in the small intestine and fermentable in the large intestine: arabinoxylan, inulin, pectin, bran, cellulose, -glucan and resistant starch. DF has an important role in improving human health. Consuming adequate quantities of DF have beneficial physiological effects: improving gastrointestinal health, decreasing cholesterolemia and glycemia, and preventing heart disease and cancer. The physiological parameters are influenced by the physicochemical properties, especially by the soluble DF fraction, as this is responsible of the changes in intestinal viscosity. Increased intestinal viscosity delays nutrient absorption, rate of passage and increases bile acid excretion. Food processing, such as flaking, has great effects on DF, especially on the molecular weight distributions. The experiments showed that on flaking, the water binding capacities and swelling capacities remained almost the same. Cereal flakes had much higher aqueous extract viscosity (AEV) than raw cereals, caused by the structural and physicochemical changes. Although total fiber values of the flakes are comparable to those of raw cereals, a redistribution of insoluble to soluble fiber occurred. Flaking resulted in an increased degradation of the molecular mass of DF. This caused a reduction in the content of insoluble DF fraction and an increase in that of the soluble fiber fraction. While increasing the solubility of -glucans and arabinoxylans, the most important water-extractable polysaccharides, an increase in AEV was also observed: 3.48 times in wheat and 4.56 times in barley. The viscosity measurement prior to ingestion can be used to predict intestinal viscosity of processed cereals. http://dx.doi.org/10.1016/j.jbiotec.2016.05.212