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Effect of growth factors IGFI, TGF-α, egf and pdgf on development of in vitro produced bovine blastocysts
291
Theriogenology EFFECT OF GROWTH FACTORS IGFI, TGF-a, EGF and PDGF ON DEVELOPMENT OF IN VITRO PRODUCED BOVINE BLASTOCYSTS G.A. Palma and G. Brem’ Bay KG, 85764 OberschleiBheim, Germany; ‘Institute of Animal Breeeding and Genetics, Veterinary University of Viemra, 1030 Vienna, Austria
The objective of this experiment was to determine the effect of growth factors (GF) Insulin like Growth Factor (IGFI, Serva, Germany), Transforming Growth Factor-alpha (TGF-a, Sigma, USA), Epidermal Growth Factor (EGF, Sigma, USA), Platelet derived Growth Factor (PDGF, ICN, USA), lOngim1 each in: 1) medium supplemented with serum, and, 2) defined culture medium without serum (mod&d Parker’s medium, MPM, Pavlok 1988, An Reprod. Sci 16:207-213) on the blastocyst development ofin vitro produced embryos. After IVM and IVF the oocytes (n = 1761) were denuded by 1 min vortexing and randomly assigned to 4 culture treatments (30 fertilized oocytes/400 ul drop): 1) MPM, 2) MPM + GF, 3) MPM + 10% estrus cow serum (ECS) and 4) MPM + GF + 10% ECS. This experiment was replicated 7 to 9 times. The embryos were observed at Day 9 and Day 13 alter fertilization to determine the number of blastocysts and hatched blastocysts. The results are shown in Table 1 Table 1. Development of IVF embryos (% of total oocytes) using different culture media, growth factors and estrus cow serum Treatment
No. of Oocytes
No of blastocysts (%)
No. of hatched blastocysts (%)
MPM
285
41 (14.4)a
88 (3.1)a
MPM+GF
574
110 (19 2)a
(s4;9’
MPM+ECS
478
139 (29 0)
98 (20 5)
MPM+ECS+GF
424
111 (26.2)b
(ll:,C
a.b,c
Numbers within the same column with different superscripts differ by Chi Square analysis (p
The results of this experiment indicate that supplementation with growth factors is beneficial to blastocyst hatching rate in absence of serum. The treatment groups supplemented with 10% ECS and 10% ECS + growth factors had the highest percentage of blastocysts and hatched blastocysts. The addition of growth factors to the MPM medium + 10% ECS had no beneficial effect on the blastocyst development rate.
Theriogenology EFFECT OF GROWTH FACTORS IGFI, TGF-a, EGF and PDGF ON DEVELOPMENT OF IN VITRO PRODUCED BOVINE BLASTOCYSTS G.A. Palma and G. Brem’ Bay KG, 85764 OberschleiBheim, Germany; ‘Institute of Animal Breeeding and Genetics, Veterinary University of Viemra, 1030 Vienna, Austria
The objective of this experiment was to determine the effect of growth factors (GF) Insulin like Growth Factor (IGFI, Serva, Germany), Transforming Growth Factor-alpha (TGF-a, Sigma, USA), Epidermal Growth Factor (EGF, Sigma, USA), Platelet derived Growth Factor (PDGF, ICN, USA), lOngim1 each in: 1) medium supplemented with serum, and, 2) defined culture medium without serum (mod&d Parker’s medium, MPM, Pavlok 1988, An Reprod. Sci 16:207-213) on the blastocyst development ofin vitro produced embryos. After IVM and IVF the oocytes (n = 1761) were denuded by 1 min vortexing and randomly assigned to 4 culture treatments (30 fertilized oocytes/400 ul drop): 1) MPM, 2) MPM + GF, 3) MPM + 10% estrus cow serum (ECS) and 4) MPM + GF + 10% ECS. This experiment was replicated 7 to 9 times. The embryos were observed at Day 9 and Day 13 alter fertilization to determine the number of blastocysts and hatched blastocysts. The results are shown in Table 1 Table 1. Development of IVF embryos (% of total oocytes) using different culture media, growth factors and estrus cow serum Treatment
No. of Oocytes
No of blastocysts (%)
No. of hatched blastocysts (%)
MPM
285
41 (14.4)a
88 (3.1)a
MPM+GF
574
110 (19 2)a
(s4;9’
MPM+ECS
478
139 (29 0)
98 (20 5)
MPM+ECS+GF
424
111 (26.2)b
(ll:,C
a.b,c
Numbers within the same column with different superscripts differ by Chi Square analysis (p
The results of this experiment indicate that supplementation with growth factors is beneficial to blastocyst hatching rate in absence of serum. The treatment groups supplemented with 10% ECS and 10% ECS + growth factors had the highest percentage of blastocysts and hatched blastocysts. The addition of growth factors to the MPM medium + 10% ECS had no beneficial effect on the blastocyst development rate.