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Effect of hyaluronan tetrasaccharides on epidermal differentiation and skin barrier function
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JSID Abstracts / Journal of Dermatological Science 69 (2013) e47–e93
lesion followed by respiratory allergy implicating allergic march. Application of acidic cream improved not only gross appearance, skin barrier functions, epidermal differentiation and allergic skin inflammation, but also airway allergic reaction compared with neutralized cream and vehicle. Our results presents that allergic march murine model could be made by Dp application on Ox induced AD model and an acidification of SC by acidic cream could prevents the allergic march formed by respiratory allergic reaction following AD skin lesions. These results indicate that a novel allergic march animal model was developed by application of house dust mite on Ox induced AD murine model and acidification of SC could be a novel intervention method to block allergic march. http://dx.doi.org/10.1016/j.jdermsci.2012.11.453 P05-31 Bilateral systematized porokeratotic eccrine ostial and dermal duct nevus showing aberrant keratin and profilaggrin expression Naoki Oiso 1,∗ , Ichiro Kurokawa 2 , Akira Kawada 1 1
Deaprtment of Dermatology, Kinki University Faculty of Medicine Department of Dermatology, Acne Clinical and Research Center, Meiwa Hospital 2
Porokeratotic eccrine ostial and dermal duct nevus (PEODDN) or porokeratotic eccrine nevus is a rare nevus characterized by hyperkeratotic streaks following the lines of Blaschko. It is recently shown to be a mosaic form of keratitis-ichthyosis-deafness syndrome caused by a mutation in the GJB2 gene encoding the gap junction protein connexin-26. We studied keratins, profilaggrin and filaggrin expression in a 1-year-old Japanese boy with bilateral systematized PEODDN. Immunohistochemical stains illustrated aberrant keratin10 and keratin 1 expression as early differentiation markers, and possible unusual excess profilaggrin expression as a terminal differentiation marker. Our results suggested that cell growth and differentiation were unregulated from early to terminal. The uncontrolled keratinization in PEODDN might be caused by the aberrant function in the gap junction that mediates intercellular communication such as cell growth and differentiation.
others expression. The interrelation of these two proteins seems to be programmed to protect skin against sunburn. http://dx.doi.org/10.1016/j.jdermsci.2012.11.455 P05-33 Effect of hyaluronan tetrasaccharides on epidermal differentiation and skin barrier function Madoka Kage ∗ , Yoshihiro Tokudome, Fumie Hashimoto Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan Hyaluronan plays a role in keratinocyte proliferation and differentiation. It has been reported that hyaluronan-mediated CD44 activation regulates keratinocyte differentiation. In this study, we aimed at investigating the influence of hyaluronan tetrasaccharides (HA4, 776.3 Da) on the regulation of keratinocyte differentiation in human keratinocytes, and compared HA4 with high molecular weight hyaluronan (HA, >1,200 kDa). In addition, the effects of HA4 on in vivo skin barrier function were examined. Human keratinocytes were treated at doses of 1 g/mL HA or HA4. Each differentiation marker mRNA expression and proteins level were detected by real-time PCR and western blotting. Hairless mice were topically treated with either 0.1% HA or HA4 in 70% ethanol or 70% ethanol alone after UVA irradiation (22.3 J/cm2 /d). Hairless mice were sacrificed by cervical dislocation after the last measurement of transepidermal water loss (TEWL). Hairless mice skin sections were stained with haematoxylin and eosin, and analyzed for structural differences using light microscopy. On day 9, late differentiation marker mRNA and protein levels were increased with HA and HA4 treatment. In addition, in the control group, TEWL and epidermal thickness were increased with UVA irradiation than the normal group. However, TEWL and epidermal thickness were decreased in the HA4 group than those of the control and HA groups. These results show that HA4 enhanced keratinocyte differentiation. In addition, HA4 improved skin functional recovery after UVA irradiation by skin penetration of HA4. That epidermal differentiation was enhanced by HA4 may be one of the factors for skin functional recovery after UVA irradiation. http://dx.doi.org/10.1016/j.jdermsci.2012.11.456
http://dx.doi.org/10.1016/j.jdermsci.2012.11.454 P05-32 Galectin-7 Interacts with HMG-CoA Synthase 1 while Inducing Its Expression Norihiro Fujimoto ∗ , Minoru Akiyama, Shingo Tajima, Takahiro Satoh Departments of Dermatology, National Defense Medical College Galectin-7 is a -galactoside binding protein specifically expressed in stratified epithelia and especially in epidermis. In this study, yeast two-hybrid genetic system was employed to identify interacted protein with human galetin-7. Three-hydroxy3-methylglutaryl coenzyme A (HMG-CoA) synthase, cytoplasmic (HMGCS1) was detected first by yeast two-hybrid genetic system, followed by confirmation with -galactosidase assay and co-immunoprecipitation. Distinct interactive site of HMGCS1 with galetin-7 was detected to reside at Phe-26 using yeast two-hybrid system and verified by His-pull down and biosensor assay. The expression of HMGCS1 was proved to be up-regulated by exogenous galectin-7 and down-regulated by galectin-7 siRNA in vitro. Moreover, Galectin-7 and HMGCS1 were revealed to enhance each
P05-34 Facial sebaceous gland size determined by three-dimensional ultrasound tomography correlates with skin elasticity Tomoko Sugawara 1,∗ , Noriaki Yoshifumi Saijo 2 , Shingo Sakai 1
Nakagawa 1 , Norio
Shimizu 1 ,
1
Innovative Beauty Science Laboratory, Kanebo cosmetics Inc. Biomedical Imaging Laboratory, Graduate School of Biomedical Engineering, Tohoku University 2
Facial skin is rich in large sebaceous glands (SGs), which change size with age. Because SGs account for a large portion of facial skin, a morphological change in them may affect the physical properties of the skin. However, the role played by SGs in the skin’s physical properties remain unclear. Three-dimensional ultrasound microscopy with a central frequency of 120 MHz is an in vivo imaging technique for human skin structure and achieves a visualization of the SG morphology non-invasively. In this study, we evaluated the relation between SG morphology and the skin’s physical properties using this technique to reveal the potential role of SGs. Sebum level, skin surface hydration, TEWL, and visco-elasticity in the cheeks of 32 healthy male subjects (mean 32 ± 3 y) were measured using Sebumeter, Skicon-200, Tewameter and Cutometer
JSID Abstracts / Journal of Dermatological Science 69 (2013) e47–e93
lesion followed by respiratory allergy implicating allergic march. Application of acidic cream improved not only gross appearance, skin barrier functions, epidermal differentiation and allergic skin inflammation, but also airway allergic reaction compared with neutralized cream and vehicle. Our results presents that allergic march murine model could be made by Dp application on Ox induced AD model and an acidification of SC by acidic cream could prevents the allergic march formed by respiratory allergic reaction following AD skin lesions. These results indicate that a novel allergic march animal model was developed by application of house dust mite on Ox induced AD murine model and acidification of SC could be a novel intervention method to block allergic march. http://dx.doi.org/10.1016/j.jdermsci.2012.11.453 P05-31 Bilateral systematized porokeratotic eccrine ostial and dermal duct nevus showing aberrant keratin and profilaggrin expression Naoki Oiso 1,∗ , Ichiro Kurokawa 2 , Akira Kawada 1 1
Deaprtment of Dermatology, Kinki University Faculty of Medicine Department of Dermatology, Acne Clinical and Research Center, Meiwa Hospital 2
Porokeratotic eccrine ostial and dermal duct nevus (PEODDN) or porokeratotic eccrine nevus is a rare nevus characterized by hyperkeratotic streaks following the lines of Blaschko. It is recently shown to be a mosaic form of keratitis-ichthyosis-deafness syndrome caused by a mutation in the GJB2 gene encoding the gap junction protein connexin-26. We studied keratins, profilaggrin and filaggrin expression in a 1-year-old Japanese boy with bilateral systematized PEODDN. Immunohistochemical stains illustrated aberrant keratin10 and keratin 1 expression as early differentiation markers, and possible unusual excess profilaggrin expression as a terminal differentiation marker. Our results suggested that cell growth and differentiation were unregulated from early to terminal. The uncontrolled keratinization in PEODDN might be caused by the aberrant function in the gap junction that mediates intercellular communication such as cell growth and differentiation.
others expression. The interrelation of these two proteins seems to be programmed to protect skin against sunburn. http://dx.doi.org/10.1016/j.jdermsci.2012.11.455 P05-33 Effect of hyaluronan tetrasaccharides on epidermal differentiation and skin barrier function Madoka Kage ∗ , Yoshihiro Tokudome, Fumie Hashimoto Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan Hyaluronan plays a role in keratinocyte proliferation and differentiation. It has been reported that hyaluronan-mediated CD44 activation regulates keratinocyte differentiation. In this study, we aimed at investigating the influence of hyaluronan tetrasaccharides (HA4, 776.3 Da) on the regulation of keratinocyte differentiation in human keratinocytes, and compared HA4 with high molecular weight hyaluronan (HA, >1,200 kDa). In addition, the effects of HA4 on in vivo skin barrier function were examined. Human keratinocytes were treated at doses of 1 g/mL HA or HA4. Each differentiation marker mRNA expression and proteins level were detected by real-time PCR and western blotting. Hairless mice were topically treated with either 0.1% HA or HA4 in 70% ethanol or 70% ethanol alone after UVA irradiation (22.3 J/cm2 /d). Hairless mice were sacrificed by cervical dislocation after the last measurement of transepidermal water loss (TEWL). Hairless mice skin sections were stained with haematoxylin and eosin, and analyzed for structural differences using light microscopy. On day 9, late differentiation marker mRNA and protein levels were increased with HA and HA4 treatment. In addition, in the control group, TEWL and epidermal thickness were increased with UVA irradiation than the normal group. However, TEWL and epidermal thickness were decreased in the HA4 group than those of the control and HA groups. These results show that HA4 enhanced keratinocyte differentiation. In addition, HA4 improved skin functional recovery after UVA irradiation by skin penetration of HA4. That epidermal differentiation was enhanced by HA4 may be one of the factors for skin functional recovery after UVA irradiation. http://dx.doi.org/10.1016/j.jdermsci.2012.11.456
http://dx.doi.org/10.1016/j.jdermsci.2012.11.454 P05-32 Galectin-7 Interacts with HMG-CoA Synthase 1 while Inducing Its Expression Norihiro Fujimoto ∗ , Minoru Akiyama, Shingo Tajima, Takahiro Satoh Departments of Dermatology, National Defense Medical College Galectin-7 is a -galactoside binding protein specifically expressed in stratified epithelia and especially in epidermis. In this study, yeast two-hybrid genetic system was employed to identify interacted protein with human galetin-7. Three-hydroxy3-methylglutaryl coenzyme A (HMG-CoA) synthase, cytoplasmic (HMGCS1) was detected first by yeast two-hybrid genetic system, followed by confirmation with -galactosidase assay and co-immunoprecipitation. Distinct interactive site of HMGCS1 with galetin-7 was detected to reside at Phe-26 using yeast two-hybrid system and verified by His-pull down and biosensor assay. The expression of HMGCS1 was proved to be up-regulated by exogenous galectin-7 and down-regulated by galectin-7 siRNA in vitro. Moreover, Galectin-7 and HMGCS1 were revealed to enhance each
P05-34 Facial sebaceous gland size determined by three-dimensional ultrasound tomography correlates with skin elasticity Tomoko Sugawara 1,∗ , Noriaki Yoshifumi Saijo 2 , Shingo Sakai 1
Nakagawa 1 , Norio
Shimizu 1 ,
1
Innovative Beauty Science Laboratory, Kanebo cosmetics Inc. Biomedical Imaging Laboratory, Graduate School of Biomedical Engineering, Tohoku University 2
Facial skin is rich in large sebaceous glands (SGs), which change size with age. Because SGs account for a large portion of facial skin, a morphological change in them may affect the physical properties of the skin. However, the role played by SGs in the skin’s physical properties remain unclear. Three-dimensional ultrasound microscopy with a central frequency of 120 MHz is an in vivo imaging technique for human skin structure and achieves a visualization of the SG morphology non-invasively. In this study, we evaluated the relation between SG morphology and the skin’s physical properties using this technique to reveal the potential role of SGs. Sebum level, skin surface hydration, TEWL, and visco-elasticity in the cheeks of 32 healthy male subjects (mean 32 ± 3 y) were measured using Sebumeter, Skicon-200, Tewameter and Cutometer