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Effect of nanosilver particles on biodegradation of laundry surfactants in urban wastewaters systems
S186
Abstracts / Toxicology Letters 229S (2014) S40–S252
Materials and methods: Acute toxicity of non polar and polar extracts of A. fragrantissima was evaluated in Albino mice. The anti-inflammatory activity of both extracts was assessed in carrageenan-induced rat paw edema model while their analgesic activity was explored centrally and peripherally using hot plate method and writhing test, respectively. In addition, the possible anti-ulcerogenic effect on colon and gastric tissues was evaluated in Wistar rats. Results: The obtained data revealed that non polar and polar extracts of A. fragrantissima showed promising anti-inflammatory, central and peripheral analgesic activities. Moreover, both fractions revealed protective effect against ulcerative colitis and gastric ulcers in rats. Conclusion: It could be concluded that non polar and polar extracts of A. fragrantissima possess promising anti-inflammatory, central and peripheral analgesic activities in addition to their protective effects to colonic and gastric tissues in varying degrees. http://dx.doi.org/10.1016/j.toxlet.2014.06.893 P11: Nanomaterials P-3.119 Effect of nanosilver particles on biodegradation of laundry surfactants in urban wastewaters systems Adela Krivohlavek 1 , Lea Ulm 1 , Irena Zˇ untar 2,∗ , 1 ˇ Ivana Vinkovic Vrcek 3 , Sandra Sikic Institute of Public Health “Dr. Andrija Sˇ tampar”, Zagreb, Croatia, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia, 3 Institute for Medical Research and Occupational Health, Zagreb, Croatia 1
ent chemical transients and thus their potential bioavailability and toxicity. http://dx.doi.org/10.1016/j.toxlet.2014.06.632 P-3.120 Cytotoxicity and genotoxicity of silver nanoparticles on isolated rat hepatocytes Mohamed Abdelaleem Faculty of Veterinary Medicine, Giza, Egypt Silver nanoparticles (AgNPs) are widely used in industrial, daily life, healthcare and medical products such as water purifiers, toys, sunscreen, wound dressings, urinary catheters and bone prostheses. So we need more studies about AgNPs adverse effects. in this study we discuss the effect of AgNPs (with mean particle size 20 ± 1.2 nm) at the cell level by using primary isolated rat hepatocyte and comparing it with the effect of silver nitrate on the same cells. we highlight the AgNPs cytotoxicity by assessing cell viability through trypan blue exclusion test, determination of enzymatic leakage percent and mitochondrial damage through analysis of ALT,LDH,AST enzymes release, assessment of oxidative stress by determination of reduced glutathione and lipid peroxides, examination of histopathological changes under light and electron microscope, and AgNPs genotoxicity through detection of DNA damage by COMET Assay and apoptosis by DNA fragmentation test. we summarize these effects on the cell which be helpful for risk assessment of AgNPs usage in the future. http://dx.doi.org/10.1016/j.toxlet.2014.06.633
2
Discharge of silver nanoparticles (AgNP) from textiles and cosmetics, todays major application areas for metallic AgNP, into wastewater is inevitable. Transformation in wastewater treatment plants (WWTP) will determine the impact of AgNP on aquatic and terrestrial environments. This study aimed to determine the behavior of AgNP in the sludge at the WWTP by addressing the stability of citrate-coated AgNPs and their interactions with laundry-relevant surfactants (sodium dodecyl sulphate (SDS) and dodecylammonium chloride (DDACl)). The stability of the AgNPs was shown to depend on the charge and concentration of the adsorbed surfactants. The presence of DDACl prevented the formation of AgNP agglomerates even at higher ionic strength. In order to determine the effect of AgNP on biodegradation of laundry surfactants, we employed standardized method for water quality and HRN-EN-ISO-10707:2000 (Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds). Additionally, we evaluated the inhibitory effect of AgNPs on the growth of activated sludge microorganisms (ASMs)). Results showed that AgNPs were toxic to ASMs at very high concentration (>50 mg/L). However, biodegradability of SDS, DDACl and commercial laundry detergent were inhibited by more than 5% and 20% in the presence of 0.1 and 1 mg/L AgNP, respectively. It can be concluded that AgNP discharged to the wastewater stream will influenced overall biodegradability of organic matter at various degrees in the sewer system. Such knowledge is important as it may influence the subsequent transport of AgNPs through differ-
P-3.121 Single particle tracking method for the analysis of intracellular movement of the silica nanoparticles Michihiko Aoyama 1,∗ , Yasuo Yoshioka 1,2 , Yoshiyuki Arai 3 , Rio Ishimoto 1 , Shin-ichi Tsunoda 2,4 , Kazuma Higashisaka 1 , Takeharu Nagai 3 , Yasuo Tsutsumi 1,4 1
Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan, 2 Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Mino, Osaka, Japan, 3 Department of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan, 4 The Center for Advanced Medical Engineering and Informatics, Osaka University, Suita, Osaka, Japan Nanomaterials (NMs) have recently been applied in various kinds of fields. In contrast, it is concerned that NMs might have unknown health risks attributed to their unique pharmacokinetics. Hence, it is needed to collect information on the detail biodistribution and intracellular dynamics of NMs for the production of safe and useful NMs. But, it was difficult to assess the intracellular movements of NMs by the conventional method because of small size. Therefore, we constructed the new system for tracking the intracellular movement of the NMs by highly inclined and laminated optical sheet method (HILO). We tracked the intracellular movement of silica particles (SPs) in the epithelial cell by using HILO. Because it is well known that particle size is an important
Abstracts / Toxicology Letters 229S (2014) S40–S252
Materials and methods: Acute toxicity of non polar and polar extracts of A. fragrantissima was evaluated in Albino mice. The anti-inflammatory activity of both extracts was assessed in carrageenan-induced rat paw edema model while their analgesic activity was explored centrally and peripherally using hot plate method and writhing test, respectively. In addition, the possible anti-ulcerogenic effect on colon and gastric tissues was evaluated in Wistar rats. Results: The obtained data revealed that non polar and polar extracts of A. fragrantissima showed promising anti-inflammatory, central and peripheral analgesic activities. Moreover, both fractions revealed protective effect against ulcerative colitis and gastric ulcers in rats. Conclusion: It could be concluded that non polar and polar extracts of A. fragrantissima possess promising anti-inflammatory, central and peripheral analgesic activities in addition to their protective effects to colonic and gastric tissues in varying degrees. http://dx.doi.org/10.1016/j.toxlet.2014.06.893 P11: Nanomaterials P-3.119 Effect of nanosilver particles on biodegradation of laundry surfactants in urban wastewaters systems Adela Krivohlavek 1 , Lea Ulm 1 , Irena Zˇ untar 2,∗ , 1 ˇ Ivana Vinkovic Vrcek 3 , Sandra Sikic Institute of Public Health “Dr. Andrija Sˇ tampar”, Zagreb, Croatia, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia, 3 Institute for Medical Research and Occupational Health, Zagreb, Croatia 1
ent chemical transients and thus their potential bioavailability and toxicity. http://dx.doi.org/10.1016/j.toxlet.2014.06.632 P-3.120 Cytotoxicity and genotoxicity of silver nanoparticles on isolated rat hepatocytes Mohamed Abdelaleem Faculty of Veterinary Medicine, Giza, Egypt Silver nanoparticles (AgNPs) are widely used in industrial, daily life, healthcare and medical products such as water purifiers, toys, sunscreen, wound dressings, urinary catheters and bone prostheses. So we need more studies about AgNPs adverse effects. in this study we discuss the effect of AgNPs (with mean particle size 20 ± 1.2 nm) at the cell level by using primary isolated rat hepatocyte and comparing it with the effect of silver nitrate on the same cells. we highlight the AgNPs cytotoxicity by assessing cell viability through trypan blue exclusion test, determination of enzymatic leakage percent and mitochondrial damage through analysis of ALT,LDH,AST enzymes release, assessment of oxidative stress by determination of reduced glutathione and lipid peroxides, examination of histopathological changes under light and electron microscope, and AgNPs genotoxicity through detection of DNA damage by COMET Assay and apoptosis by DNA fragmentation test. we summarize these effects on the cell which be helpful for risk assessment of AgNPs usage in the future. http://dx.doi.org/10.1016/j.toxlet.2014.06.633
2
Discharge of silver nanoparticles (AgNP) from textiles and cosmetics, todays major application areas for metallic AgNP, into wastewater is inevitable. Transformation in wastewater treatment plants (WWTP) will determine the impact of AgNP on aquatic and terrestrial environments. This study aimed to determine the behavior of AgNP in the sludge at the WWTP by addressing the stability of citrate-coated AgNPs and their interactions with laundry-relevant surfactants (sodium dodecyl sulphate (SDS) and dodecylammonium chloride (DDACl)). The stability of the AgNPs was shown to depend on the charge and concentration of the adsorbed surfactants. The presence of DDACl prevented the formation of AgNP agglomerates even at higher ionic strength. In order to determine the effect of AgNP on biodegradation of laundry surfactants, we employed standardized method for water quality and HRN-EN-ISO-10707:2000 (Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds). Additionally, we evaluated the inhibitory effect of AgNPs on the growth of activated sludge microorganisms (ASMs)). Results showed that AgNPs were toxic to ASMs at very high concentration (>50 mg/L). However, biodegradability of SDS, DDACl and commercial laundry detergent were inhibited by more than 5% and 20% in the presence of 0.1 and 1 mg/L AgNP, respectively. It can be concluded that AgNP discharged to the wastewater stream will influenced overall biodegradability of organic matter at various degrees in the sewer system. Such knowledge is important as it may influence the subsequent transport of AgNPs through differ-
P-3.121 Single particle tracking method for the analysis of intracellular movement of the silica nanoparticles Michihiko Aoyama 1,∗ , Yasuo Yoshioka 1,2 , Yoshiyuki Arai 3 , Rio Ishimoto 1 , Shin-ichi Tsunoda 2,4 , Kazuma Higashisaka 1 , Takeharu Nagai 3 , Yasuo Tsutsumi 1,4 1
Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan, 2 Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Mino, Osaka, Japan, 3 Department of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan, 4 The Center for Advanced Medical Engineering and Informatics, Osaka University, Suita, Osaka, Japan Nanomaterials (NMs) have recently been applied in various kinds of fields. In contrast, it is concerned that NMs might have unknown health risks attributed to their unique pharmacokinetics. Hence, it is needed to collect information on the detail biodistribution and intracellular dynamics of NMs for the production of safe and useful NMs. But, it was difficult to assess the intracellular movements of NMs by the conventional method because of small size. Therefore, we constructed the new system for tracking the intracellular movement of the NMs by highly inclined and laminated optical sheet method (HILO). We tracked the intracellular movement of silica particles (SPs) in the epithelial cell by using HILO. Because it is well known that particle size is an important