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Effect of potentiated interferon alpha on tumour cell growth
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Effect of potentiated interferon alpha on tumour cell growth M. BARTHEL The study was carried out in the Department of Applied Microbiology, University of Lublin, Poland, by Martina Kandefer, Esbieta Malarczyk and Michael Barthel. Like other cytokines interferons are physiologically produced in very low concentrations in the body and have a multiple effect on practically all cells, e.g. growth regulation, immune functions or resistance to tumour development. The antiproliferative effect on normal and tumour cells is various and is achieved by inhibiting the formation of growth factor-inducible genes. The aim of the in-vitro study was to investigate the effect of very high dilutions of human interferon alpha 88 produced from Escherichia coli (IFN) on the growth of the human lung carcinoma cell line A 549. The antiviral and antiproliferating activity of the IFN was evaluated by counting the number of cells and the protein content. Interferon can stimulate and inhibit the antiviral resistance of cells, as was shown in our experiments with dilutions of up to 1 : 1080. The results also show the importance of vigorous shaking for the activity of very high dilutions in comparison with merely using a pipette without shaking, in which case no activity was observed with higher dilutions than 1 : 104.
British Homoeopathic Journal
was still conspicuous presence of cartilage and also some fibrous tissue at the fracture site. In the animals which received either of the two dilutions of Arnica and Symphytum, and irrespective of the mode of administration, both X-rays and histology showed completely mineralized new bone through the entire fracture site in 33% of the fractures. The experiments were repeated 3 times, with the same result.
Effect of in-ovo administration of high dilutions of bursin in bursectomized chicks B.J.YOUBICIER-SIMO, EBoUDARD, M.GUELLATI,J.D BAYLI~,M.BASTIDE
Early embryonic surgical removal of the Fabricius bursa (normal site of B lymphocyte differentiation in birds) alters basal and stress-induced adrenocortical activity in newly hatched chicks. Chicks bursectomized as early embryos can produce different classes of immunoglobulins (IgM, IgG and IgA) but are unable to generate specific antibodies in response to antigenic stimulation. The aim was to study the effect of high dilutions of bursin, a tripeptide synthesized by the bursal epithelium, in restoring hormonal and specific humoral immune responses in bursectomized chicks. Controls and treated chicks received 3 injections of porcine thyroglobulin (Tg) as antigen on days 21, 30 and 39 after hatching. 18 of 240 bursectomized chickens survived. Plasma corticosterone levels progressively increased on days 29 and 38 (8 days after the 1st and 2nd injections of Tg) and then Effect of Arnica montana and Symphytum returned to basal values. No variation of cortiofficinalis on bone healing in guinea pigs costerone concentrations could be detected M. OBERBAUM,E. YAKOVLEV,D. KAUFMAN, after repeated immunizations of bursectoS. SHOSHAN mized chickens treated with saline, whereas The present preliminary study was designed chicks injected with bursin as embryos to test our working hypothesis according to responded to Tg injections. Anti-Tg antibody which the administration of both Arnica and levels were very low on day 20 in all groups, Symphytum to guinea pigs, following experi- before the first immunization. Low IgG anti mentally induced bone fractures, would both Tg values were found in bursectomized chickaccelerate and improve healing. ens after immunization. Sham operated chickThe ulnae of 35 guinea pigs were fractured ens exhibited very high IgG anti Tg titres. In under general anaesthesia. The fracture site ovo administration of bursin to bursectowas then covered by the retracted muscles. mized embryos re-established immune reacThe incision was sutured and the wound tivity in adult birds no matter what amounts of sprayed with Nobecutane. The intact radius tripeptide bursin were injected. The speciserved as an immobilization splint. ficity of IgG antibodies was assessed by incuThe results showed that by X-ray criteria bation of the sera with various antigens such none of the untreated controls or saline- as OVA, BSA, MYO, A C T and Tg. Then the treated fractures were completely mineral- residual activity anti-Tg was tested again by ized. Histological examination revealed there indirect E L I S A using Tg as the antigen. No
Effect of potentiated interferon alpha on tumour cell growth M. BARTHEL The study was carried out in the Department of Applied Microbiology, University of Lublin, Poland, by Martina Kandefer, Esbieta Malarczyk and Michael Barthel. Like other cytokines interferons are physiologically produced in very low concentrations in the body and have a multiple effect on practically all cells, e.g. growth regulation, immune functions or resistance to tumour development. The antiproliferative effect on normal and tumour cells is various and is achieved by inhibiting the formation of growth factor-inducible genes. The aim of the in-vitro study was to investigate the effect of very high dilutions of human interferon alpha 88 produced from Escherichia coli (IFN) on the growth of the human lung carcinoma cell line A 549. The antiviral and antiproliferating activity of the IFN was evaluated by counting the number of cells and the protein content. Interferon can stimulate and inhibit the antiviral resistance of cells, as was shown in our experiments with dilutions of up to 1 : 1080. The results also show the importance of vigorous shaking for the activity of very high dilutions in comparison with merely using a pipette without shaking, in which case no activity was observed with higher dilutions than 1 : 104.
British Homoeopathic Journal
was still conspicuous presence of cartilage and also some fibrous tissue at the fracture site. In the animals which received either of the two dilutions of Arnica and Symphytum, and irrespective of the mode of administration, both X-rays and histology showed completely mineralized new bone through the entire fracture site in 33% of the fractures. The experiments were repeated 3 times, with the same result.
Effect of in-ovo administration of high dilutions of bursin in bursectomized chicks B.J.YOUBICIER-SIMO, EBoUDARD, M.GUELLATI,J.D BAYLI~,M.BASTIDE
Early embryonic surgical removal of the Fabricius bursa (normal site of B lymphocyte differentiation in birds) alters basal and stress-induced adrenocortical activity in newly hatched chicks. Chicks bursectomized as early embryos can produce different classes of immunoglobulins (IgM, IgG and IgA) but are unable to generate specific antibodies in response to antigenic stimulation. The aim was to study the effect of high dilutions of bursin, a tripeptide synthesized by the bursal epithelium, in restoring hormonal and specific humoral immune responses in bursectomized chicks. Controls and treated chicks received 3 injections of porcine thyroglobulin (Tg) as antigen on days 21, 30 and 39 after hatching. 18 of 240 bursectomized chickens survived. Plasma corticosterone levels progressively increased on days 29 and 38 (8 days after the 1st and 2nd injections of Tg) and then Effect of Arnica montana and Symphytum returned to basal values. No variation of cortiofficinalis on bone healing in guinea pigs costerone concentrations could be detected M. OBERBAUM,E. YAKOVLEV,D. KAUFMAN, after repeated immunizations of bursectoS. SHOSHAN mized chickens treated with saline, whereas The present preliminary study was designed chicks injected with bursin as embryos to test our working hypothesis according to responded to Tg injections. Anti-Tg antibody which the administration of both Arnica and levels were very low on day 20 in all groups, Symphytum to guinea pigs, following experi- before the first immunization. Low IgG anti mentally induced bone fractures, would both Tg values were found in bursectomized chickaccelerate and improve healing. ens after immunization. Sham operated chickThe ulnae of 35 guinea pigs were fractured ens exhibited very high IgG anti Tg titres. In under general anaesthesia. The fracture site ovo administration of bursin to bursectowas then covered by the retracted muscles. mized embryos re-established immune reacThe incision was sutured and the wound tivity in adult birds no matter what amounts of sprayed with Nobecutane. The intact radius tripeptide bursin were injected. The speciserved as an immobilization splint. ficity of IgG antibodies was assessed by incuThe results showed that by X-ray criteria bation of the sera with various antigens such none of the untreated controls or saline- as OVA, BSA, MYO, A C T and Tg. Then the treated fractures were completely mineral- residual activity anti-Tg was tested again by ized. Histological examination revealed there indirect E L I S A using Tg as the antigen. No