Effect of preexposure to endotoxins in cat allergic asthmatics

S58 Abstracts

J ALLERGY CLIN IMMUNOL FEBRUARY 2004

SATURDAY 133

Effect of Preexposure to Endotoxins in Cat Allergic Asthmatics

C. Sohy1, F. Lieutier-Colas1, A. Casset1, P. Meyer2, G. Pauli1, F. de Blay1; 1Hôpitaux Universitaires de Strasbourg, Service de Pneumologie, Strasbourg, FRANCE, 2Faculté de Médecine, Service de Biostatistiques et Informatique Médicale, Strasbourg, FRANCE. RATIONALE: Inhalation of 200 µg of lipopolysaccharide (LPS) in nor-

mal subjects and 20 µg in asthmatics induces a decrease FEV1 and an increase level of the baseline nonspecific bronchial hyperresponsiveness. The aim was to determine the effect of prior inhalation of lower dose of LPS (corresponding to domestic airborne exposure in Strasbourg) on immediate bronchial response in mild cat allergic asthmatics. METHODS: Twenty subjects with an intermittent asthma sensitized to cat were included. Each subjects underwent 2 randomized cat allergen bronchial challenge (CABCT) test sessions precede (24 h), in double blind, by a “prior” nebulization of saline solution or 2 µg of LPS. RESULTS: There were no significantly difference between the parameters (FEV1, PEF, FEF 25-75) measured during the CABCTs when test performed after saline solution or LPS (p=0.173, 0.428, 0.839, respectively). There was no significant differences between PD20 Fel d1 after LPS versus saline solution exposure (p=0.42). No significant difference was seen in late bronchial response. CONCLUSIONS: Our study demonstrated that exposure to low level of LPS (approximate the domestic airborne exposure in Strasbourg) did not enhance significantly airway responsiveness to cat allergen in cat allergic asthmatics. Funding: Grant Monies

Abstracts S59

SATURDAY

J ALLERGY CLIN IMMUNOL VOLUME 113, NUMBER 2