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Effect of purified saikosaponin derivatives upon antibody response against T-dependent and -independent antigen in mice
146
IMMUNOPHARMACOLOGY
Vol. i0, No. I
MECHANISM OF IMMUNE SUPPRESSION AGAINST SHEEP ERYTHROCYTE BY S O M E SAIKOSAPONIN DERIVATIVES. H. K o h n o , M. T a w a r a , N. Y a m a g u c h i , H. A b e * , S. O d a s h i m a a n d T. S a i t o . K a n a z a w a M e d i c a l u n i v e r s i t y , Uchinada, Ishikawa, and Kinki University, Osaka, Japan. In a f o r m e r e x p e r i m e n t , purified saikosaponin derivatives, especially a a n d d, w e r e o b s e r v e d to s u p p r e s s antibody formation against sheep erythrocytes(T-dependent antigen), but they augmented antibody formation to b a c t e r i a l LPS(T-independent antigen) From the above results, it is c l e a r t h a t t h e s e d r u g s s t i m u l a t e B-cells. W h a t is t h e r e a s o n w h y a n t i b o d y formation by SRBC was not provoked, in s p i t e of e n h a n c e m e n t of B - c e l l s t i m u l a t i o n ? It is i n f e r r e d t h a t s u p p r e s s i v e T-cells are also induced and affected the PFC generation. In o r d e r to t e s t t h i s p o s s i b i l i t y , spleen cells were separated from a drug-treated host and passively transferred into a recipient. Spleen cells from treated host effectively suppressed the anti-SRBC P F C in t h e r e c i p i e n t but not by serum. Then the spleen cells were divided into adherent and nonadherent c e l l s , a n d e a c h of t h e m w a s t r a n s f e r r e d into a recipient Suppressive activity was clearly demonstrated in a n o n - a d h e r e n t cell pupolation. Further characterization of t h e l y m p h o c y t e sub)opulation is u n d e r i n v e s t i g a t i o n . Together with the enhancing e f f e c t on m a c r o p h a g e activity, this China herb, Saikosaponin, augments the activity of m a j o r l y m p h o c y t e subpopulations s u c h as T- a n d B - c e l l s .
EFFECT OF PURIFIED SAIKOSAPONIN DERIVATIVES UPON ANTIBODY RESPONSE AGAINST T-DEPENDENT AND -INDEPENDENT ANTIGEN IN MICE. S. Odashima, H. Kohno, M. Tawara, H. Abe ~, N. Yamaguchi and T. Saito. Kanazawa Medical University, Uehinada, Ishikawa, and Kinki University ~, Osaka, Japan. Effect of the purified derivatives from the root of the plant(Bupletum falcatum L.) upon the immune response of BALB/c mice was investigated against T-dependent(sheep erythrocyte;SRBC) and T-independent antigens(bacterial lipopolysaccharide;LPS). This herb is famous for its anti-inflammation, anti-tumor and the other biological effects, and has been widely used in a solitary or complexed form from ancient times in Oriental countries. The effects on immune responsivenss of these drugs given prior to antigen administration were judged by the number of plaque forming cells(PFC) in the spleen. The purified derivatives, Saikosaponin(SS)a and d, suppressed antiSRBC PFC but enhanced the anti-LPS PFC. The other derivatives, b , b and 2 [,showed no effect on anti-SRBC and anti-LPS PFC. From the experlment establishing an effective dose for anti-SRBC PFC formation in mice, one mg/kg was observed to be optimum for BALB/c mice. Although the phenotypic effect of this drug as judged by PFC against T-dependent antigen was not identified, it is suggested that the purified derivatives SSa and d stimulated both Tand B-cells. It is inferred that suppressive ceTls as-well as helper cells are generated before antigenic stimulation, and the activity of suppressor T-cells is superior to that of helper T-cells.
IMMUNOPHARMACOLOGY
Vol. i0, No. I
MECHANISM OF IMMUNE SUPPRESSION AGAINST SHEEP ERYTHROCYTE BY S O M E SAIKOSAPONIN DERIVATIVES. H. K o h n o , M. T a w a r a , N. Y a m a g u c h i , H. A b e * , S. O d a s h i m a a n d T. S a i t o . K a n a z a w a M e d i c a l u n i v e r s i t y , Uchinada, Ishikawa, and Kinki University, Osaka, Japan. In a f o r m e r e x p e r i m e n t , purified saikosaponin derivatives, especially a a n d d, w e r e o b s e r v e d to s u p p r e s s antibody formation against sheep erythrocytes(T-dependent antigen), but they augmented antibody formation to b a c t e r i a l LPS(T-independent antigen) From the above results, it is c l e a r t h a t t h e s e d r u g s s t i m u l a t e B-cells. W h a t is t h e r e a s o n w h y a n t i b o d y formation by SRBC was not provoked, in s p i t e of e n h a n c e m e n t of B - c e l l s t i m u l a t i o n ? It is i n f e r r e d t h a t s u p p r e s s i v e T-cells are also induced and affected the PFC generation. In o r d e r to t e s t t h i s p o s s i b i l i t y , spleen cells were separated from a drug-treated host and passively transferred into a recipient. Spleen cells from treated host effectively suppressed the anti-SRBC P F C in t h e r e c i p i e n t but not by serum. Then the spleen cells were divided into adherent and nonadherent c e l l s , a n d e a c h of t h e m w a s t r a n s f e r r e d into a recipient Suppressive activity was clearly demonstrated in a n o n - a d h e r e n t cell pupolation. Further characterization of t h e l y m p h o c y t e sub)opulation is u n d e r i n v e s t i g a t i o n . Together with the enhancing e f f e c t on m a c r o p h a g e activity, this China herb, Saikosaponin, augments the activity of m a j o r l y m p h o c y t e subpopulations s u c h as T- a n d B - c e l l s .
EFFECT OF PURIFIED SAIKOSAPONIN DERIVATIVES UPON ANTIBODY RESPONSE AGAINST T-DEPENDENT AND -INDEPENDENT ANTIGEN IN MICE. S. Odashima, H. Kohno, M. Tawara, H. Abe ~, N. Yamaguchi and T. Saito. Kanazawa Medical University, Uehinada, Ishikawa, and Kinki University ~, Osaka, Japan. Effect of the purified derivatives from the root of the plant(Bupletum falcatum L.) upon the immune response of BALB/c mice was investigated against T-dependent(sheep erythrocyte;SRBC) and T-independent antigens(bacterial lipopolysaccharide;LPS). This herb is famous for its anti-inflammation, anti-tumor and the other biological effects, and has been widely used in a solitary or complexed form from ancient times in Oriental countries. The effects on immune responsivenss of these drugs given prior to antigen administration were judged by the number of plaque forming cells(PFC) in the spleen. The purified derivatives, Saikosaponin(SS)a and d, suppressed antiSRBC PFC but enhanced the anti-LPS PFC. The other derivatives, b , b and 2 [,showed no effect on anti-SRBC and anti-LPS PFC. From the experlment establishing an effective dose for anti-SRBC PFC formation in mice, one mg/kg was observed to be optimum for BALB/c mice. Although the phenotypic effect of this drug as judged by PFC against T-dependent antigen was not identified, it is suggested that the purified derivatives SSa and d stimulated both Tand B-cells. It is inferred that suppressive ceTls as-well as helper cells are generated before antigenic stimulation, and the activity of suppressor T-cells is superior to that of helper T-cells.