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Effect of selenopiran on immune system under stress conditions
STRE3SLLMlTING AND IMMUNOMODULATING EFFECT OF MENTAL TRAINING P. Bundzen, E. Gavrilova, V. Isakov and L. - E. Unestahl
ROLE OF VIRAL INFJXTIONS DURING THE COURSE OF COMPLICATED LEUKEMIA 0. Botzova, L. Vissokovskaya, V. Zotova, E. Polevichenko, L. Fisenko, E. Efremova.
Research Phy,sical Culture Institute, S-Petersburg, Russia, Scandinavian International (Jniversity, Orehro, Sweden
Regional ChiIdren Hospital, Medical Umversity Rostov - on - Don. Russia
The purpose of investigation was to study the physiological effects of mental training. Experimental group (EG, 28 subjects) used the Russian version of Swedish model of mental training. The total duration of the course formed 8 weeks. The control group (CG, 10 subjects) listened to relaxation music only. The study of the program included the test POMS (MC Nair, 1992), the determination of cortisol and P-endorphin levels in blood serum by radioimmunological method and the level of free fat acids (FFA) by the photocolorimetrical method. There determined the level of immunoglobulins A, G, M and the state of cellular immunity. In EG there occurs a considerable reduce of the level of anxiety (p
Viral infection is one of major cause for the deteriorating course of leukemia in the immunocompromised patients. 166 children with acute lymphoblastic leukemia (ALL) were examined for immune status and level of antibodies to HCV, CMV, HSV and HBS antigen. Examination of Tlymphocyte subpopulations indicated their redistribution: decrease of CD16+, CD3+ and CD4+ cells and increase of CDS+ cells (CD4+/CDS+ = 0,87). During the therapy counts of T-lymphocytes were significantly increased (p
BLOCKING OF PHOSPHOLIPASE C AND AI1 HAEMOLYTIC ACTIVITY WITH THE HELP OF TNF-a AND C-REACTIVE PROTEIN A. Butyugov, P. Nazarov, Y. Lyubimov
EFFECT OF SELENOPIRAN ON IMMUNE SYSTEM UNDER STRESS CONDITIONS G. E. Boryaev, A. F. Blinokhvatov The Penza State Agricultural Academy, Penza, Russia
Department of Immunology, Institute for Experimental Medicine and Institute for Toxicology, St. Petersburg, Russia
The experiments reported here indicate the effect of selenopiran (9-phenil-symmetricoctahydroselenoxanthene) synthesised by the authors on immune system under stress conditions. Selenopiran was intramuscular injected to 3month old weaning lambs at dose levels of 0,l mg of Se/kg of live weight. The changes in the lambs’ immune systems were studied. IgG and IgM were found in in blood serum by radial immunodiffusion with application of antichain serum and monoclonal antibodies; IgA was found by enzyme multiplied immunoassay. Bactericidal activity of the serum and granulocytes phagocytic activity were also determined. Postweaning period in a lamb life s characterised by stress condition resulting in decreasing IgM and IgA concentrations. The experiment showed that injection of selenopiran sharply reduced negative stress effect on immune system. It also indicated that serum IgM concentration decreased by 0.28 mg/ccm. and serum IgA concentration decreased by 0.49 mg/ccm. in control group of lambs on the 10” day after weaning, while in experimental group of animals - by 0.06 mg/ccm. and 0.01 mg/ccm. respectively. The bactericidal activity decreased by 8% (and by 28% in control group of animals). The protective effect of selenopiran is supposed to be :onnected with reducing stress effect by free radical oxidation adjusting. Sodium selenite had no such effect.
Several toxic shock syndromes are associated with elevated serum concentrations of different types of phospholipases, that may serve as toxic stress factors. TNF-a and C-reactive protein (CRP) play a key role in developing of immune response during early phase of infection. The aim of the study was to assess the ability of CRP and recombinant human TNF-a to modify haemolytic activity of phospholipase C (PLC) from Clperfringens and phospholipase All (PLAII) from Apis mellifera. Different concentrations of CRP and TNF-a were incubated with PLC and PLAII during 30 mm at 37’C, and then, 1% suspension of human red blood cells was added in reaction mixtures. Results obtained showed that CRP inhibits haemolytic activity of PLs, with K,=233 y/ml and 132,3 y/ml for PLAII and PLC, correspondingly. TNF-a displayed inhibition of haemolytic activity of PLAlI, but it was concentrations depended: enhancement of haemolysis at 450 U/ml and higher and inhibition at lower concentration of TNF-a.The results indicate that inhibition of haemolytic activity of PLs by CRP have stable and non-competitive mode, in contrast with TNF-c( effect.
148
ROLE OF VIRAL INFJXTIONS DURING THE COURSE OF COMPLICATED LEUKEMIA 0. Botzova, L. Vissokovskaya, V. Zotova, E. Polevichenko, L. Fisenko, E. Efremova.
Research Phy,sical Culture Institute, S-Petersburg, Russia, Scandinavian International (Jniversity, Orehro, Sweden
Regional ChiIdren Hospital, Medical Umversity Rostov - on - Don. Russia
The purpose of investigation was to study the physiological effects of mental training. Experimental group (EG, 28 subjects) used the Russian version of Swedish model of mental training. The total duration of the course formed 8 weeks. The control group (CG, 10 subjects) listened to relaxation music only. The study of the program included the test POMS (MC Nair, 1992), the determination of cortisol and P-endorphin levels in blood serum by radioimmunological method and the level of free fat acids (FFA) by the photocolorimetrical method. There determined the level of immunoglobulins A, G, M and the state of cellular immunity. In EG there occurs a considerable reduce of the level of anxiety (p
Viral infection is one of major cause for the deteriorating course of leukemia in the immunocompromised patients. 166 children with acute lymphoblastic leukemia (ALL) were examined for immune status and level of antibodies to HCV, CMV, HSV and HBS antigen. Examination of Tlymphocyte subpopulations indicated their redistribution: decrease of CD16+, CD3+ and CD4+ cells and increase of CDS+ cells (CD4+/CDS+ = 0,87). During the therapy counts of T-lymphocytes were significantly increased (p
BLOCKING OF PHOSPHOLIPASE C AND AI1 HAEMOLYTIC ACTIVITY WITH THE HELP OF TNF-a AND C-REACTIVE PROTEIN A. Butyugov, P. Nazarov, Y. Lyubimov
EFFECT OF SELENOPIRAN ON IMMUNE SYSTEM UNDER STRESS CONDITIONS G. E. Boryaev, A. F. Blinokhvatov The Penza State Agricultural Academy, Penza, Russia
Department of Immunology, Institute for Experimental Medicine and Institute for Toxicology, St. Petersburg, Russia
The experiments reported here indicate the effect of selenopiran (9-phenil-symmetricoctahydroselenoxanthene) synthesised by the authors on immune system under stress conditions. Selenopiran was intramuscular injected to 3month old weaning lambs at dose levels of 0,l mg of Se/kg of live weight. The changes in the lambs’ immune systems were studied. IgG and IgM were found in in blood serum by radial immunodiffusion with application of antichain serum and monoclonal antibodies; IgA was found by enzyme multiplied immunoassay. Bactericidal activity of the serum and granulocytes phagocytic activity were also determined. Postweaning period in a lamb life s characterised by stress condition resulting in decreasing IgM and IgA concentrations. The experiment showed that injection of selenopiran sharply reduced negative stress effect on immune system. It also indicated that serum IgM concentration decreased by 0.28 mg/ccm. and serum IgA concentration decreased by 0.49 mg/ccm. in control group of lambs on the 10” day after weaning, while in experimental group of animals - by 0.06 mg/ccm. and 0.01 mg/ccm. respectively. The bactericidal activity decreased by 8% (and by 28% in control group of animals). The protective effect of selenopiran is supposed to be :onnected with reducing stress effect by free radical oxidation adjusting. Sodium selenite had no such effect.
Several toxic shock syndromes are associated with elevated serum concentrations of different types of phospholipases, that may serve as toxic stress factors. TNF-a and C-reactive protein (CRP) play a key role in developing of immune response during early phase of infection. The aim of the study was to assess the ability of CRP and recombinant human TNF-a to modify haemolytic activity of phospholipase C (PLC) from Clperfringens and phospholipase All (PLAII) from Apis mellifera. Different concentrations of CRP and TNF-a were incubated with PLC and PLAII during 30 mm at 37’C, and then, 1% suspension of human red blood cells was added in reaction mixtures. Results obtained showed that CRP inhibits haemolytic activity of PLs, with K,=233 y/ml and 132,3 y/ml for PLAII and PLC, correspondingly. TNF-a displayed inhibition of haemolytic activity of PLAlI, but it was concentrations depended: enhancement of haemolysis at 450 U/ml and higher and inhibition at lower concentration of TNF-a.The results indicate that inhibition of haemolytic activity of PLs by CRP have stable and non-competitive mode, in contrast with TNF-c( effect.
148