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Effect of successive application of secondary sludge from pulp mill in the soil-plant ecosystem
New Biotechnology · Volume 29S · September 2012
an issue of major concern. Heavy metals are the major toxicants found in industrial waste water. In this study, cobalt-tolerant bacteria were isolated from various locations of Kızılırmak along the city Kırıkkale, Turkey. Cobalt-tolerant isolate with a minimal inhibitory concentration of 750 mg L−1 was isolated. Identification of the bacterium was carried out using biochemical tests and 16S rDNA sequencing. The isolate was shown to be resistant to other heavy metals like cadmium, aluminum, lithium, barium, manganese, lead, silver, tin and nickel. Plasmid DNA analysis results revealed that the cobalt resistance ability of this river isolate was chromosome-encoded. Outer membrane and total protein isolation results descriptively showed that not only membrane porins but also other proteins were effective in cobalt resistance. Keywords: Cobalt-tolerant bacteria; Molecular characterization; 16S DNA sequencing; Heavy metal resistance http://dx.doi.org/10.1016/j.nbt.2012.08.652 Poster 5.0.213 Effect of successive application of secondary sludge from pulp mill in the soil-plant ecosystem F. Gallardo∗ , C. Oyarce, M.C. Diez Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, PO Box 54-D, Temuco, Chile Secondary sludge is produced in wastewater treatment plant of cellulose mill that need be disposed without risk for the environment. This sludge has a high organic matter content, macro and micronutrients, providing a material with a high quality to be used as fertilizer in agriculture. Sludge application has been proven in international studies; however, we have not experience in Chile about its application at field scale. Therefore, we studied the effect of repeated application of this sludge on soil chemical and biological properties and in vegetal production of Lolium perenne L. var Banquet. We used soils derived from volcanic ashes localized in Southern Chile. For the experimental design, 12 plots of 6 m × 2 m were establish and sludge was added in the dosage of 0, 10, 20 and 30 Mg ha−1 , with three repetitions and four applications every 90 days. Monthly cuts were done to extract the vegetable samples in field. Then, samples were analyzed to measure P-Olsen’s level of the soil, the productivity and the level of P in foliar and radical biomass of L. perenne L. The successive application of sludge increased the Olsen-P levels in soil in comparison to the control as well as the vegetable production of L. perenne L. Also, levels of P in biomass increased, because the high amount of this nutrient available to the plant. In addition, L. perenne L., improved coverage on the meadow increasing the number of clump/m2 . Besides, sludge application improved biological and chemical properties of the soil. Acknowledgements: This research was financed by FONDECYT Project 1080427, DIUFRO DI11-1001, and INNOVA-CORFO 09FC02-6021. http://dx.doi.org/10.1016/j.nbt.2012.08.653
Poster 5.0.214 Ethic assessments on biosafety for Turkey Melike Baran Ekinci∗ , Bedia Simsek Isparta Food Control Laboratory, Turkey There are three important issues related to ethics on biosafety; one of them is that there is not ethic non-labeling product, if there is GM product, the other is that non-ethics approaches on gene transfer and GM products are used for food aid. GM labeling came into force in all EU Member States. The GM Food and Feed Regulation (EC) No. 1829/2003 lays down rules to cover all GM food and animal feed, regardless of the presence of any GM material in the final product. This means products such as flour, oils and glucose syrups will have to label as GM if they are from a GM source. But products such as meat, milk and eggs from animals fed on GM animal feed will also not need to be labeled. Products produced with GM technology (cheese produced with GM enzymes, for example) will not have to be labeled too. Any intentional use of GM ingredients at any level must be labeled. But there is no need for small amounts of GM ingredients (below 0.9% for approved GM varieties and 0.5% for unapproved GM varieties that have received a favorable assessment from an EC scientific committee) that are accidentally present in a food to be labeled. Turkey regulation was harmonized with EU directives. According to Biosafety Law (No.5977, 2010), a ethic sub-committee was also built by the biosafety committee to determination ethics situation in Turkey. Keywords: Labeling; Ethics; Biosafety http://dx.doi.org/10.1016/j.nbt.2012.08.654 Poster 5.0.215 Molecular characterization of zinc-resistant bacteria isolated from industrial effluents Akbulut Sevilay, Orman Nazlı∗ , Icgen Bulent Kırıkkale University, Department of Biology, 71450-Kırıkkale, Turkey Zinc resistance was investigated in strains isolated from the river Kızılırmak which has been affected by industrial and agricultural heavy metal pollution from the surrounding facilities and domestic effluents from the city Kırıkkale, Turkey. Zinc-resistant isolate with a minimal inhibitory concentration of 825 mg L−1 was isolated. Identification of the bacteria was carried out using biochemical tests and 16S rDNA sequencing. The isolate was shown to be resistant to other heavy metals like aluminum, lithium, copper, silver, nickel and strontium and resistance to the antibiotics like gentamicin, trimeth-sulfa and pefloxacin. Plasmid DNA analysis results revealed that the zinc resistance ability of this river isolate was chromosome-encoded. Outer membrane and total protein isolation results descriptively showed that not only membrane porins but also other proteins were effective in zinc resistance. Keywords: Zinc-resistant bacteria; Molecular characterization; 16S rDNA sequencing; Heavy metal resistance http://dx.doi.org/10.1016/j.nbt.2012.08.655
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www.elsevier.com/locate/nbt
an issue of major concern. Heavy metals are the major toxicants found in industrial waste water. In this study, cobalt-tolerant bacteria were isolated from various locations of Kızılırmak along the city Kırıkkale, Turkey. Cobalt-tolerant isolate with a minimal inhibitory concentration of 750 mg L−1 was isolated. Identification of the bacterium was carried out using biochemical tests and 16S rDNA sequencing. The isolate was shown to be resistant to other heavy metals like cadmium, aluminum, lithium, barium, manganese, lead, silver, tin and nickel. Plasmid DNA analysis results revealed that the cobalt resistance ability of this river isolate was chromosome-encoded. Outer membrane and total protein isolation results descriptively showed that not only membrane porins but also other proteins were effective in cobalt resistance. Keywords: Cobalt-tolerant bacteria; Molecular characterization; 16S DNA sequencing; Heavy metal resistance http://dx.doi.org/10.1016/j.nbt.2012.08.652 Poster 5.0.213 Effect of successive application of secondary sludge from pulp mill in the soil-plant ecosystem F. Gallardo∗ , C. Oyarce, M.C. Diez Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, PO Box 54-D, Temuco, Chile Secondary sludge is produced in wastewater treatment plant of cellulose mill that need be disposed without risk for the environment. This sludge has a high organic matter content, macro and micronutrients, providing a material with a high quality to be used as fertilizer in agriculture. Sludge application has been proven in international studies; however, we have not experience in Chile about its application at field scale. Therefore, we studied the effect of repeated application of this sludge on soil chemical and biological properties and in vegetal production of Lolium perenne L. var Banquet. We used soils derived from volcanic ashes localized in Southern Chile. For the experimental design, 12 plots of 6 m × 2 m were establish and sludge was added in the dosage of 0, 10, 20 and 30 Mg ha−1 , with three repetitions and four applications every 90 days. Monthly cuts were done to extract the vegetable samples in field. Then, samples were analyzed to measure P-Olsen’s level of the soil, the productivity and the level of P in foliar and radical biomass of L. perenne L. The successive application of sludge increased the Olsen-P levels in soil in comparison to the control as well as the vegetable production of L. perenne L. Also, levels of P in biomass increased, because the high amount of this nutrient available to the plant. In addition, L. perenne L., improved coverage on the meadow increasing the number of clump/m2 . Besides, sludge application improved biological and chemical properties of the soil. Acknowledgements: This research was financed by FONDECYT Project 1080427, DIUFRO DI11-1001, and INNOVA-CORFO 09FC02-6021. http://dx.doi.org/10.1016/j.nbt.2012.08.653
Poster 5.0.214 Ethic assessments on biosafety for Turkey Melike Baran Ekinci∗ , Bedia Simsek Isparta Food Control Laboratory, Turkey There are three important issues related to ethics on biosafety; one of them is that there is not ethic non-labeling product, if there is GM product, the other is that non-ethics approaches on gene transfer and GM products are used for food aid. GM labeling came into force in all EU Member States. The GM Food and Feed Regulation (EC) No. 1829/2003 lays down rules to cover all GM food and animal feed, regardless of the presence of any GM material in the final product. This means products such as flour, oils and glucose syrups will have to label as GM if they are from a GM source. But products such as meat, milk and eggs from animals fed on GM animal feed will also not need to be labeled. Products produced with GM technology (cheese produced with GM enzymes, for example) will not have to be labeled too. Any intentional use of GM ingredients at any level must be labeled. But there is no need for small amounts of GM ingredients (below 0.9% for approved GM varieties and 0.5% for unapproved GM varieties that have received a favorable assessment from an EC scientific committee) that are accidentally present in a food to be labeled. Turkey regulation was harmonized with EU directives. According to Biosafety Law (No.5977, 2010), a ethic sub-committee was also built by the biosafety committee to determination ethics situation in Turkey. Keywords: Labeling; Ethics; Biosafety http://dx.doi.org/10.1016/j.nbt.2012.08.654 Poster 5.0.215 Molecular characterization of zinc-resistant bacteria isolated from industrial effluents Akbulut Sevilay, Orman Nazlı∗ , Icgen Bulent Kırıkkale University, Department of Biology, 71450-Kırıkkale, Turkey Zinc resistance was investigated in strains isolated from the river Kızılırmak which has been affected by industrial and agricultural heavy metal pollution from the surrounding facilities and domestic effluents from the city Kırıkkale, Turkey. Zinc-resistant isolate with a minimal inhibitory concentration of 825 mg L−1 was isolated. Identification of the bacteria was carried out using biochemical tests and 16S rDNA sequencing. The isolate was shown to be resistant to other heavy metals like aluminum, lithium, copper, silver, nickel and strontium and resistance to the antibiotics like gentamicin, trimeth-sulfa and pefloxacin. Plasmid DNA analysis results revealed that the zinc resistance ability of this river isolate was chromosome-encoded. Outer membrane and total protein isolation results descriptively showed that not only membrane porins but also other proteins were effective in zinc resistance. Keywords: Zinc-resistant bacteria; Molecular characterization; 16S rDNA sequencing; Heavy metal resistance http://dx.doi.org/10.1016/j.nbt.2012.08.655
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www.elsevier.com/locate/nbt