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Effect of titanium surface modification by nitriding on osteoblastic cell adhesion and spreading
Abstracts that strengthen and support many tissues, including cartilage and bone, so the high expression of these genes in healthy pulp tissue, suggest their possible involvement in guided bone regeneration using stem cells isolated from dental pulp. Conflict of interest: None declared.
383 Inducing human bone marrow to a safety autologous bioreactor suitable for bone regeneration
doi:10.1016/j.ijom.2011.07.644
Introduction: Stem cells based therapies are the most promising and powerful method to provide patients specific tissue substitutes for the regeneration or reconstruction of tissue and organs. Aim: Using bone marrow stimulation for maxillofacial bone reconstruction surgery. Design: We have developed and original process to awake the bone marrow in humans, the last being considered as a physiological bioreactor to produce autologous stem cells. Patient and method: We injected a combination of autologous platelet richplasma solution added with recombinant human soluble tissue factor (Rhstf) in the posterior eliac crest. Applied on 13 patients. Results: Our trial showed the feasibility and harmless of the procedure. Whereas no modification of stem cell phenotype was noticed, an increase of medullar cellularity was observed on the day 3 after injection. Endothelial progenitor cells (EPC) were mobilized to the bloodstream without stimulation of hematopoietic stem cells (HSC). Mesenchymal stem cells (Msc) count in bone marrow increased with a specific commitment to preosteoblastic cell population both in vivo and in vitro. Conclusion: Human bone marrow can be safety used as a bioreactors for focused stem cells production. Conflict of interest: None declared.
382 Effect of titanium surface modification by nitriding on osteoblastic cell adhesion and spreading E. Ferraz 1,∗ , A. Sverzut 1 , P.T. De Oliveira 1 , C. Alves 2 , A.L. Rosa 1 1 Universidade de São Paulo – USP, Ribeirão Preto, Brazil 2 Universidade Federal do Rio Grande do Norte, Natal, Brazil
Introduction: Titanium surface modifications can influence the osseointegration process, the final goal being accelerate bone formation around implants. Purpose: This study aimed at evaluating the effect of Ti surface modification by nitriding on osteoblastic cell adhesion and spreading. Material and methods: Plasma nitriding treatments were carried out in Ti discs using atmosphere with 20% N2 and 80% H2 at 450 ◦ C and 3 different conditions of pressure and time (Group 1: 1.5 mbar for 1 h; Group 2: 2.6 mbar for 1 h; Group3: 2.6 mbar for 3 h). Untreated discs were used as control. Osteoblastic cells derived from human alveolar bone were cultured on Ti discs for 2 h, fixed and stained with phalloidin (cytoskeleton) and DAPI (nuclei). Under fluorescence microscopy, cell adhesion was evaluated by counting the number of cells in 10 fields under 20× of magnification and cell spreading was evaluated by measuring the area of 40 cells, for each Ti surface. Data were compared by ANOVA means followed by Tuckey test (p < 0.05). Results: Both cell adhesion and spreading were significantly lower in Group 3 while Groups 1 and 2 displayed values similar to control. Conclusion: These results suggest that nitriding under higher pressure for periods longer than 1 hour produces Ti surfaces that disturb the earlier osteoblastic cell behaviors such as adhesion and spreading. Financial support: FAPESP and CNPq. Conflict of interest: None declared.
P.R. Philippart Maxillo Facial, IRIS South Public Hospital of Brussels, Brussels, Belgium
doi:10.1016/j.ijom.2011.07.646
384 Surgical anatomy of the swine face for maxillofacial surgery R. Sasaki 1,2,∗ , Y. Watanabe 2,3 , H. Matsumine 4 , M. Yamato 2 , T. Okano 2 , T. Ando 1 1 Department of Oral and Maxillofacial Surgery and Global Center of Excellence (COE) Program, Japan 2 Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Japan 3 Department of Plastic and Reconstructive Surgery, Tokyo Metropolitan Police Hospital, Tokyo, Japan 4 Department of Plastic Surgery, Yachiyo Medical Center, Tokyo Women’s Medical University, Chiba, Japan
Objective: Animal models offer an unprecedented opportunity to preclinically evaluate the efficacy and safety of newly developed human surgical therapies. In order to develop maxillofacial surgical research including tissue engineering and face transplantation, the surgical anatomy of the miniature pig’s face was investigated using identical procedures that were previously described by Ellis and Zide for humans. Methods: A modified Blair incision was initially made through the facial skin and subcutaneous tissues. The underlying tissues were then dissected in order to progressively expose the platysma muscle, the superficial layer of deep cervical fascia, the facial artery and vein, the facial nerve, the mental nerve, and the facial skeleton. Results: The marginal mandibular branch of the facial nerve has an upper and lower division. Stimulation of the facial nerve and its branches showed that the upper division of marginal mandibular branch innervates muscles and tissues in the upper lip and nose region, and the lower division innervates muscles and tissues in the lower lip region. The condyle of mandible has only one head and no condylar process. Orbit has no orbital floor and lateral wall. Conclusion: The gross anatomy of the maxillofacial region in the pigs was found to be similar to that of humans. Although the distributions of the marginal mandibular branch of the facial nerve, the condyle and the orbit are different from that of humans, we concluded that miniature pigs are suitable experimental model for the preclinical development of tissue engineering applications and face transplantation in maxillofacial surgery. Conflict of interest: None declared. doi:10.1016/j.ijom.2011.07.647
doi:10.1016/j.ijom.2011.07.645
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383 Inducing human bone marrow to a safety autologous bioreactor suitable for bone regeneration
doi:10.1016/j.ijom.2011.07.644
Introduction: Stem cells based therapies are the most promising and powerful method to provide patients specific tissue substitutes for the regeneration or reconstruction of tissue and organs. Aim: Using bone marrow stimulation for maxillofacial bone reconstruction surgery. Design: We have developed and original process to awake the bone marrow in humans, the last being considered as a physiological bioreactor to produce autologous stem cells. Patient and method: We injected a combination of autologous platelet richplasma solution added with recombinant human soluble tissue factor (Rhstf) in the posterior eliac crest. Applied on 13 patients. Results: Our trial showed the feasibility and harmless of the procedure. Whereas no modification of stem cell phenotype was noticed, an increase of medullar cellularity was observed on the day 3 after injection. Endothelial progenitor cells (EPC) were mobilized to the bloodstream without stimulation of hematopoietic stem cells (HSC). Mesenchymal stem cells (Msc) count in bone marrow increased with a specific commitment to preosteoblastic cell population both in vivo and in vitro. Conclusion: Human bone marrow can be safety used as a bioreactors for focused stem cells production. Conflict of interest: None declared.
382 Effect of titanium surface modification by nitriding on osteoblastic cell adhesion and spreading E. Ferraz 1,∗ , A. Sverzut 1 , P.T. De Oliveira 1 , C. Alves 2 , A.L. Rosa 1 1 Universidade de São Paulo – USP, Ribeirão Preto, Brazil 2 Universidade Federal do Rio Grande do Norte, Natal, Brazil
Introduction: Titanium surface modifications can influence the osseointegration process, the final goal being accelerate bone formation around implants. Purpose: This study aimed at evaluating the effect of Ti surface modification by nitriding on osteoblastic cell adhesion and spreading. Material and methods: Plasma nitriding treatments were carried out in Ti discs using atmosphere with 20% N2 and 80% H2 at 450 ◦ C and 3 different conditions of pressure and time (Group 1: 1.5 mbar for 1 h; Group 2: 2.6 mbar for 1 h; Group3: 2.6 mbar for 3 h). Untreated discs were used as control. Osteoblastic cells derived from human alveolar bone were cultured on Ti discs for 2 h, fixed and stained with phalloidin (cytoskeleton) and DAPI (nuclei). Under fluorescence microscopy, cell adhesion was evaluated by counting the number of cells in 10 fields under 20× of magnification and cell spreading was evaluated by measuring the area of 40 cells, for each Ti surface. Data were compared by ANOVA means followed by Tuckey test (p < 0.05). Results: Both cell adhesion and spreading were significantly lower in Group 3 while Groups 1 and 2 displayed values similar to control. Conclusion: These results suggest that nitriding under higher pressure for periods longer than 1 hour produces Ti surfaces that disturb the earlier osteoblastic cell behaviors such as adhesion and spreading. Financial support: FAPESP and CNPq. Conflict of interest: None declared.
P.R. Philippart Maxillo Facial, IRIS South Public Hospital of Brussels, Brussels, Belgium
doi:10.1016/j.ijom.2011.07.646
384 Surgical anatomy of the swine face for maxillofacial surgery R. Sasaki 1,2,∗ , Y. Watanabe 2,3 , H. Matsumine 4 , M. Yamato 2 , T. Okano 2 , T. Ando 1 1 Department of Oral and Maxillofacial Surgery and Global Center of Excellence (COE) Program, Japan 2 Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Japan 3 Department of Plastic and Reconstructive Surgery, Tokyo Metropolitan Police Hospital, Tokyo, Japan 4 Department of Plastic Surgery, Yachiyo Medical Center, Tokyo Women’s Medical University, Chiba, Japan
Objective: Animal models offer an unprecedented opportunity to preclinically evaluate the efficacy and safety of newly developed human surgical therapies. In order to develop maxillofacial surgical research including tissue engineering and face transplantation, the surgical anatomy of the miniature pig’s face was investigated using identical procedures that were previously described by Ellis and Zide for humans. Methods: A modified Blair incision was initially made through the facial skin and subcutaneous tissues. The underlying tissues were then dissected in order to progressively expose the platysma muscle, the superficial layer of deep cervical fascia, the facial artery and vein, the facial nerve, the mental nerve, and the facial skeleton. Results: The marginal mandibular branch of the facial nerve has an upper and lower division. Stimulation of the facial nerve and its branches showed that the upper division of marginal mandibular branch innervates muscles and tissues in the upper lip and nose region, and the lower division innervates muscles and tissues in the lower lip region. The condyle of mandible has only one head and no condylar process. Orbit has no orbital floor and lateral wall. Conclusion: The gross anatomy of the maxillofacial region in the pigs was found to be similar to that of humans. Although the distributions of the marginal mandibular branch of the facial nerve, the condyle and the orbit are different from that of humans, we concluded that miniature pigs are suitable experimental model for the preclinical development of tissue engineering applications and face transplantation in maxillofacial surgery. Conflict of interest: None declared. doi:10.1016/j.ijom.2011.07.647
doi:10.1016/j.ijom.2011.07.645
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