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Effect of whey proteins and their constitutive peptides or aminoacids mix on growth steatorrhea and nitrogen balance after acute starvation in the rat
P.19
EARLY ENTERAL SUPPORT MAINTAINS MUCOSAL INTEGRITY AFTER BURNS. H.C. Sax, 0. Trocki H. Arita, C.K. Ogle, J.W. Alexander (Department of Surgery, University of Cincinnati and Shriners Hospital for Crippled Children, Burn Unit, Cincinnati, Ohio, USA). The post-burn period is marked by dramatic increases in metabolic rate. Recent work has suggested that the institution of early (within 2 hours) enteral feeds significantly blunts this increase, perhaps by maintaining gut mucosal integrity. The escape of enteric bacterial products may be helpful. Female Hartley guinea pigs underwent gastrostomy, followed 7 days later by 30% flame burn. The groups were subdivided into those fed immediately after burn (IF) or those whose feedings were delayed 24 hours (DF). The tube feeding was infused at 175 kcal/kg/ day. It contained 12% fat, 20% protein, and 68% carbohydrate. An additional 1% of the amino acid arginine was included. On post-burn day (PBD) 1, 3 and 7 animals were sacrificed. Gut weight was determined and portal blood was quantitatively assayed for endotoxin, utilizing a chromogenic substrate. Portal vein endotoxin (pg/ml + SEM) was: control (n=lO) 4.4kO.9; PBDl, DF (n=7) 5.120.9; PBD3, IF (n=6) 7.623.2, OF (n=9) 12.252.8, ~~0.05 vs. all groups; PBD7, IF (n=7) 5.1t1.5, DF (n=8) 5.3~0.8. Gut mucosal weight in ileum (g/10 cm) was: control 0.40+0.02; PBDl 0.32iO.04; PBD3, IF 0.40?0.03, DF 0.31?0.03; PBD7, IF 0.39kO.03, DF 0.3620.06. There was a transient increase in portal endotoxin 3 days post-burn in the animals starved for 24 hours post-injury. Although gut mucosal weight appears low in this group, significance was not reached (p=O.17). Gut mucosal weight returned toward control values by PBD7, with lower portal endotoxin concentrations. Preliminary data reveals that direct portal infusion of endotoxin in intact animals induces a mild hypermetabolism. We hypothesize that the transient escape of endotoxin from the gut may be the trigger mechanism in post-burn hypermetabolism.
P.20
EFFECT OF WHEY PROTEINS AND THEIR CONSTITUTIVE PEPTIDES OR AMINOACIDS MIX ON GROWTH STEATORRHEA AND NITROGEN BALANCE AFTER ACUTE STARVATION IN THE RAT. M.G. Poullain*, L. Roger***, F. Mendy***,JG# Gobert**, J.P. Cbzard* (+INSERM U.120, Paris ***Sopharga RU, France **Lab. Coprologie, Fer a Moulin, Paris A different jejunal adaptation (morphology and disaccharidases) has been observed when whey proteins were given as whole proteins (P) or controled trypsin and chymotrypsin peptides hydrolysate (PH) or as amino-acids mix (Ah) after acute starvation (60h) in the rat. In this study we determined their effects on growth (G), steatorrhea (S) fecal and urinary nitrogen excretion (FN, UN) in 36 Wistar rats (250-300gr) after 60h of S and 24, 48, 72 and 96h of realimentation (R). The three diets had the same caloric, nitrogen carbohydrate, lipid, vitamin and mineral contents. Rats had free access to the liquid diet but had to eat the appropriate quantity (90 calories+lO%/day) to be included in the study. No difference was observed for steatorrhea and fecal nitrogen with an absorption rate over 95% during the 96h of R with the 3 liquid synthetic diets. In contrast weight increase after 60h S (-13%) was statistically better with PH (+9% after 96h R) as compare to P (+5%) and AA (+2%). This was associated with a statistically higher nitrogen balance for PH, a consequence of a lower nitrogen urinary excretion (table). R 96 h R 48 h R 72 h R 24 h S Control P
416351x
1:
,,"~~5~
P PH Nitrogen balance AA (mg/24h) x, xx p
461254 825+10x 472238
Nitrogen urinary excretion (mg/24h)
215255
322263
326+96 68+-23x 3042122 567+94 831+23x 577+125
375285 82+32x 434+109 516+86 818+32x 463+110
386+102 107+21x 7o4+83xx 5142120 792Z21X 19O+84xx
451+90 116+-37x 676+63xx 439290 744+35x 209+64"
The lower urinary nitrogen excretion with PH determine a better growth and protein anabolism. This could be correlated to a better intestinal absorption kinetic and bioavaibility of amino-acids with PH as compare to P or AA mix.
106
EARLY ENTERAL SUPPORT MAINTAINS MUCOSAL INTEGRITY AFTER BURNS. H.C. Sax, 0. Trocki H. Arita, C.K. Ogle, J.W. Alexander (Department of Surgery, University of Cincinnati and Shriners Hospital for Crippled Children, Burn Unit, Cincinnati, Ohio, USA). The post-burn period is marked by dramatic increases in metabolic rate. Recent work has suggested that the institution of early (within 2 hours) enteral feeds significantly blunts this increase, perhaps by maintaining gut mucosal integrity. The escape of enteric bacterial products may be helpful. Female Hartley guinea pigs underwent gastrostomy, followed 7 days later by 30% flame burn. The groups were subdivided into those fed immediately after burn (IF) or those whose feedings were delayed 24 hours (DF). The tube feeding was infused at 175 kcal/kg/ day. It contained 12% fat, 20% protein, and 68% carbohydrate. An additional 1% of the amino acid arginine was included. On post-burn day (PBD) 1, 3 and 7 animals were sacrificed. Gut weight was determined and portal blood was quantitatively assayed for endotoxin, utilizing a chromogenic substrate. Portal vein endotoxin (pg/ml + SEM) was: control (n=lO) 4.4kO.9; PBDl, DF (n=7) 5.120.9; PBD3, IF (n=6) 7.623.2, OF (n=9) 12.252.8, ~~0.05 vs. all groups; PBD7, IF (n=7) 5.1t1.5, DF (n=8) 5.3~0.8. Gut mucosal weight in ileum (g/10 cm) was: control 0.40+0.02; PBDl 0.32iO.04; PBD3, IF 0.40?0.03, DF 0.31?0.03; PBD7, IF 0.39kO.03, DF 0.3620.06. There was a transient increase in portal endotoxin 3 days post-burn in the animals starved for 24 hours post-injury. Although gut mucosal weight appears low in this group, significance was not reached (p=O.17). Gut mucosal weight returned toward control values by PBD7, with lower portal endotoxin concentrations. Preliminary data reveals that direct portal infusion of endotoxin in intact animals induces a mild hypermetabolism. We hypothesize that the transient escape of endotoxin from the gut may be the trigger mechanism in post-burn hypermetabolism.
P.20
EFFECT OF WHEY PROTEINS AND THEIR CONSTITUTIVE PEPTIDES OR AMINOACIDS MIX ON GROWTH STEATORRHEA AND NITROGEN BALANCE AFTER ACUTE STARVATION IN THE RAT. M.G. Poullain*, L. Roger***, F. Mendy***,JG# Gobert**, J.P. Cbzard* (+INSERM U.120, Paris ***Sopharga RU, France **Lab. Coprologie, Fer a Moulin, Paris A different jejunal adaptation (morphology and disaccharidases) has been observed when whey proteins were given as whole proteins (P) or controled trypsin and chymotrypsin peptides hydrolysate (PH) or as amino-acids mix (Ah) after acute starvation (60h) in the rat. In this study we determined their effects on growth (G), steatorrhea (S) fecal and urinary nitrogen excretion (FN, UN) in 36 Wistar rats (250-300gr) after 60h of S and 24, 48, 72 and 96h of realimentation (R). The three diets had the same caloric, nitrogen carbohydrate, lipid, vitamin and mineral contents. Rats had free access to the liquid diet but had to eat the appropriate quantity (90 calories+lO%/day) to be included in the study. No difference was observed for steatorrhea and fecal nitrogen with an absorption rate over 95% during the 96h of R with the 3 liquid synthetic diets. In contrast weight increase after 60h S (-13%) was statistically better with PH (+9% after 96h R) as compare to P (+5%) and AA (+2%). This was associated with a statistically higher nitrogen balance for PH, a consequence of a lower nitrogen urinary excretion (table). R 96 h R 48 h R 72 h R 24 h S Control P
416351x
1:
,,"~~5~
P PH Nitrogen balance AA (mg/24h) x, xx p
461254 825+10x 472238
Nitrogen urinary excretion (mg/24h)
215255
322263
326+96 68+-23x 3042122 567+94 831+23x 577+125
375285 82+32x 434+109 516+86 818+32x 463+110
386+102 107+21x 7o4+83xx 5142120 792Z21X 19O+84xx
451+90 116+-37x 676+63xx 439290 744+35x 209+64"
The lower urinary nitrogen excretion with PH determine a better growth and protein anabolism. This could be correlated to a better intestinal absorption kinetic and bioavaibility of amino-acids with PH as compare to P or AA mix.
106