poly-l -lactic acid material by ultraviolet treatment

349 activity in vitro with low ␮M IC50s and inhibited the growth of oral cancer cell lines. Findings: Among the inhibitors, PiB, had the least nonspecific toxicity. Conclusion: These results suggest that Pin1 inhibitors could be used as a novel type of anticancer drug that acts by blocking cell cycle progression. http://dx.doi.org/10.1016/j.ijom.2017.02.1174 Effect on surface character and mechanical property of unsintered hydroxyapatite/poly-l-lactic acid material by ultraviolet treatment A. Moroi ∗ , H. Ikawa, K. Yoshizawa, K. Ueki University of Yamanashi, Chuo, Japan Background: Materials fabricated with unsintered hydroxyapatite/poly-l-lactic acid (uHA/PLLA) exhibit hydrophobic. This hydrophilicity limits the ability of cells or tissues to adhere to the surface of the material and thereby prevents the materials from exhibiting effective biological activity. Objectives: In this study, we examined the effects of ultraviolet (UV) treatment on the hydrophobicity of uHA/PLLA. Methods: The plate and membrane contained a 40 wt% fraction of raw uHA particles in PLLA (uHA/PLLA; Super-FIXSORB-MX; Takiron Co. Ltd., Osaka, Japan). Materials were treated with UV light (172 nm wavelength, 13 mW/cm2 ) for 8 min; control materials were not treated. The hydrophilic properties were evaluated by measuring the contact angle after dropping the water on the material surface. Bending strength was measured by the threepoint bending test. Evaluating cell attachment ability, cells were seeded in six-well tissue plates. The treated and untreated membranes were placed in the wells 2 h after seeding. Attachment of cells was determined based on crystal violet assays. Findings and Conclusion: The contact angle of UV-treated uHA/PLLA was significantly reduced compared with that of untreated uHA/PLLA. However, there were no differences in mechanical strength. Notably, the ability of cells to adhere to UVtreated uHA/PLLA was significantly increased compared with that of untreated uHA/PLLA. These findings demonstrated that UV irradiation of uHA/PLLA was improved the surface hydrophilicity without changing the mechanical strength of the material; thus, UV treatment of uHA/PLLA may facilitate the use of this material in biomedical applications. http://dx.doi.org/10.1016/j.ijom.2017.02.1175 Third molar position on panoramic radiographs and its location perioperatively J.N. Nogueira Neto ∗ , J.F.C. Dantas, D.M. De Paula, G.M. Paraguassú, R.F. De Souza, V.A. Sarmento Federal University of Bahia, Brazil Background: The Pell and Gregory and Winter classifications in panoramic radiographs are routinely used to determine the position of third molars and to plan surgical procedures. Objectives: This study aimed to relate the position of upper and lower third molars evaluated by panoramic radiographs using the

Pell and Gregory and Winter classifications to their position during extraction surgery. Methods: The placement of 123 upper and lower third molars was assessed radiographically and perioperatively. The data were tabulated and the association of variables was tested by the Kappa (κ) index for an error probability of 5%. Findings: By comparing the correlation between the position on the radiographs and perioperatively, it was observed that for the lower third molars, using the Pell and Gregory classification in relation to the mandibular, and cervical versus the occlusal plane of the second molar and the Winter classification, agreement was acceptable (κ = 0.54, κ = 0.49, κ = 0.41 respectively), and statistically significant (P < 0). Conclusion: Even with an acceptable degree of agreement between the position of third molars on panoramic radiographs using the Pell and Gregory and Winter classifications and perioperatively, the possibility of error in evaluating the spatial position exists. The difficulty of the procedure may be underestimated or surgically overestimated, leading to the need for changes in the surgical plan during the procedure. http://dx.doi.org/10.1016/j.ijom.2017.02.1176 High-fat diet and progression of temporomandibular joint osteoarthritis in mice T. Oguma ∗ , N. Ogi, Y. Sugita, H. Maeda, K. Kurita Aichi Gakuin University, Nagoya, Japan Background: Obesity is a risk factor for osteoarthritis even in nonweight-bearing joints. However, the effects of body-fat factors on progression of temporomandibular joint (TMJ) osteoarthritis have not been investigated. In the current study, TMJ osteoarthritis was experimentally induced with discectomy in mice and compared based on a normal or a high-fat diet. Objectives: We examined association of high-fat diet with progression of TMJ osteoarthritis in mice. Methods: Male C57BL/6j mice at four weeks of age were placed on a normal diet or high-fat diet. Body weight and blood glucose of all the mice were recorded every month. At 12 weeks of age, unilateral discs of TMJ were removed under the stereomicroscope. At 16 weeks post-discectomy, the animals were killed and TMJ were taken out en bloc. TMJ osteoarthritis was evaluated by cartilage histology. The experimental procedures and protocol were approved by the University Ethical Committee. Findings: TMJ of mice fed the high-fat diet showed increased osteoarthritis degeneration compared to mice fed the normal diet. High-fat diet group significantly gained weight more than the normal diet group. There were hyperglycosemia during feeding period in the high-fat diet group. Articular cartilage degeneration was seen in both groups. Articular cartilage thickness became thinner in the high-fat diet group than in the normal diet group. The high-fat diet group tended to exhibit prominent disturbance of chondrocytes and reduction of Safranin-O staining. Conclusion: These results suggest that the obesity induced by high-fat diet increases the severity of cartilage degeneration in the TMJ osteoarthritis model. http://dx.doi.org/10.1016/j.ijom.2017.02.1177