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Effects of Antimicrobial Peptide Cathelicidin (Ll-37) by 625 nm Led Irradiation in Porphyromonas Gingivalis Infected Immortalized Gingival Fibroblasts
ORAL AND MAXILLOFACIAL PATHOLOGY e168 Abstracts its distinct histopathological and immunohistochemical features and differential diagnosis. Keywords: Myoepithelioma, salivary gland tumor, cribriform pattern, case report
SEVERE RECURRENT GINGIVAL HYPERPLASIA IN A 7-YEAR-OLD PATIENT Kate Parker, Muhammed Almomen, Peter Ayliffe, Robert Evans, Maxillofacial and Dental Department, Great Ormond Street Hospital, London, UK A 7-year-old boy has been managed at Great Ormond Street Hospital since infancy for severe recurrent gingival hyperplasia. The patient first presented at 18 months of age with severe gingival hyperplasia covering the upper and lower dentition, causing aesthetic and functional concerns. There was no family history of neurofibromatosis or other medical conditions associated with gingival hyperplasia. Medically, the patient has multiple sutural synostoses; however, no causes of the gingival hyperplasia were identified. Multiple gingivetcomies and the exposure of teeth have been performed over a period of 5 years. Histopathological diagnoses have alternated between juvenile hyaline fibromatosis and gingival fibromatosis, with the patient still being investigated for a confirmed aetiology. This case report outlines the diagnostic difficulties of this case and highlights the patient management and histopathological findings over a 5-year period. This is a rare case of paediatric severe recurrent gingival hyperplasia where the aetiology remains unknown. Keywords: Gingival Hyperplaisa, Paediatric, Juvenile Hyaline Fibromatosis
EFFECTS OF ANTIMICROBIAL PEPTIDE CATHELICIDIN (LL-37) BY 625 NM LED IRRADIATION IN PORPHYROMONAS GINGIVALIS INFECTED IMMORTALIZED GINGIVAL FIBROBLASTS Okjoon Kim, Sangwoo Kim, Jisun Kim, Hongran Choi, Department of Oral Pathology, Dental Science Research Institute and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea Objective: Antimicrobial peptide are crucial to the host defense response in maintaining cellular homeostasis against microbial invasion. Light emitting diode (LED) irradiation affects the host defense response against bacterial infections. Study Design: We investigated the host defense response by LED irradiation of P. gingivalis infected immortalized gingival fibroblasts (IGFs). IGFs were exposed to P. gingivalis following LED irradiation at 425, 525 and 625 nm (11 mW/cm2 for 1 h). The ‘Dark 1’ group had IGFs only and the ‘Dark 2’ group contained P. gingivlais infected IGFs without irradiation. Results: LED irradiation showed bactericidal activity via the LL-37 antimicrobial peptide in P. gingivalis infected IGFs. Irradiation at 625 nm decreased inflammatory responses involving prostaglandin E2 release induced by ROS in P. gingivlais infected IGFs. Conclusions: Irradiation at 625 nm is important in the host anti-inflammatory defense response involving an antimicrobial peptide in P. gingivalis infected IGFs. Keywords: Antimicrobial peptide, cathelicidin (LL-37), Porphyromonas gingivalis (P. gingivalis), LED irradiation (425, 525 and 625nm)
PSEUDO-GAUCHER CELLS IN UNDIAGNOSED THALASSEMIA PATIENT: A CASE REPORT Firstine Kelsi Hartanto1, Ajura Abdul Jalil2, Noor Azwani Mat Nawi3,
OOOO March 2015 Lau Shin Hin2, 1Department of Oro-Maxillofacial Surgical & Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia; 2Stomatology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia; 3Oral Surgery Department, Queen Elizabeth Hospital, Kota Kinabalu Sabah, Malaysia Gaucher-like or pseudo-Gaucher cells can occur in many conditions. They may cause diagnostic confusion, but it can be a clue of pathological conditions including chronic myeloid leukemia, type II congenital dyserythropoietic anemia and thalassemia. Histologically, pseudo-Gaucher cells have a lobulated and eccentric nucleus with foamy cytoplasm. We report a 16-year old female who presented with facial bone enlargement and also under follow-up with medical team to rule out b-Thalassemia. Histopathological examination of a biopsy for mandibular bone swelling showed presence of sheets of pseudo-Gaucher cells in the marrow spaces. The finding of these pseudo-Gaucher cells can be helpful to establish diagnosis and further management of this patient. Keywords: Pseudo-Gaucher cells, Thalassemia, mandible
AGE ESTIMATION IN CHILDREN BY MEASUREMENT OF OPEN APICES IN TOOTH ROOTS IN CHINESE POPULATION IN MALAYSIA: A RADIOLOGIC STUDY Renjith Pallivathukal George, Preethy Mary Donald, Sumanth Kumbargere Nagraj, Htoo Htoo Kyawe, Faculty of Dentistry, Melaka Manipal Medical College, Melaka, Malaysia Objective: Age estimation is used in the absence of accurate age data, forensic dentistry in human identification, paediatric endocrinology and in orthodontic treatment. This pilot study is done to test the accuracy of Cameriere’s age estimation method using European formula for Chinese children in Malaysia. Study Design: We evaluated the Orthopantomographs of 45 Chinese children (17 boys and 28 girls) aged between 5 to 18 years using Romexis 3.0.1R software. Dental age was calculated based on the European formula. Results: Pearson correlation between the chronological age and dental age was significant at 0.01 level with P value ¼ 0.909. Mean of difference of chronological age and dental age is 0.5547 years and the mean +/-2SD is 3.3234 to -2.214 years. Conclusions: Dental age calculated using the European formula showed high and significant correlation with the chronological age therefore the formula can be applied to the Chinese population in Malaysia. Keywords: Camriere, open apex, dental age, Chinese, European formula
ANGIOGENESIS OF ORAL LICHEN PLANUS: AN IMMUNOHISTOCHEMICAL STUDY Merva Soluk Tekkes¸in1, Alper Sinanoglu2, Vakur Olgac1, 1Department of Tumour Pathology, Institute of Oncology, Istanbul University, Istanbul, Turkey; 2Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Kocaeli University, Kocaeli, Turkey Objective: Oral lichen planus (OLP) is a relatively common disease and considered to be a chronic inflammatory disease with an autoimmune pathogenesis. Angiogenesis plays an important role in both physiological and pathologic conditions, including chronic inflammatory disorders. The aim of this immunohistochemical study was to investigate the role of angiogenesis in the pathogenesis of OLP.
SEVERE RECURRENT GINGIVAL HYPERPLASIA IN A 7-YEAR-OLD PATIENT Kate Parker, Muhammed Almomen, Peter Ayliffe, Robert Evans, Maxillofacial and Dental Department, Great Ormond Street Hospital, London, UK A 7-year-old boy has been managed at Great Ormond Street Hospital since infancy for severe recurrent gingival hyperplasia. The patient first presented at 18 months of age with severe gingival hyperplasia covering the upper and lower dentition, causing aesthetic and functional concerns. There was no family history of neurofibromatosis or other medical conditions associated with gingival hyperplasia. Medically, the patient has multiple sutural synostoses; however, no causes of the gingival hyperplasia were identified. Multiple gingivetcomies and the exposure of teeth have been performed over a period of 5 years. Histopathological diagnoses have alternated between juvenile hyaline fibromatosis and gingival fibromatosis, with the patient still being investigated for a confirmed aetiology. This case report outlines the diagnostic difficulties of this case and highlights the patient management and histopathological findings over a 5-year period. This is a rare case of paediatric severe recurrent gingival hyperplasia where the aetiology remains unknown. Keywords: Gingival Hyperplaisa, Paediatric, Juvenile Hyaline Fibromatosis
EFFECTS OF ANTIMICROBIAL PEPTIDE CATHELICIDIN (LL-37) BY 625 NM LED IRRADIATION IN PORPHYROMONAS GINGIVALIS INFECTED IMMORTALIZED GINGIVAL FIBROBLASTS Okjoon Kim, Sangwoo Kim, Jisun Kim, Hongran Choi, Department of Oral Pathology, Dental Science Research Institute and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea Objective: Antimicrobial peptide are crucial to the host defense response in maintaining cellular homeostasis against microbial invasion. Light emitting diode (LED) irradiation affects the host defense response against bacterial infections. Study Design: We investigated the host defense response by LED irradiation of P. gingivalis infected immortalized gingival fibroblasts (IGFs). IGFs were exposed to P. gingivalis following LED irradiation at 425, 525 and 625 nm (11 mW/cm2 for 1 h). The ‘Dark 1’ group had IGFs only and the ‘Dark 2’ group contained P. gingivlais infected IGFs without irradiation. Results: LED irradiation showed bactericidal activity via the LL-37 antimicrobial peptide in P. gingivalis infected IGFs. Irradiation at 625 nm decreased inflammatory responses involving prostaglandin E2 release induced by ROS in P. gingivlais infected IGFs. Conclusions: Irradiation at 625 nm is important in the host anti-inflammatory defense response involving an antimicrobial peptide in P. gingivalis infected IGFs. Keywords: Antimicrobial peptide, cathelicidin (LL-37), Porphyromonas gingivalis (P. gingivalis), LED irradiation (425, 525 and 625nm)
PSEUDO-GAUCHER CELLS IN UNDIAGNOSED THALASSEMIA PATIENT: A CASE REPORT Firstine Kelsi Hartanto1, Ajura Abdul Jalil2, Noor Azwani Mat Nawi3,
OOOO March 2015 Lau Shin Hin2, 1Department of Oro-Maxillofacial Surgical & Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia; 2Stomatology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia; 3Oral Surgery Department, Queen Elizabeth Hospital, Kota Kinabalu Sabah, Malaysia Gaucher-like or pseudo-Gaucher cells can occur in many conditions. They may cause diagnostic confusion, but it can be a clue of pathological conditions including chronic myeloid leukemia, type II congenital dyserythropoietic anemia and thalassemia. Histologically, pseudo-Gaucher cells have a lobulated and eccentric nucleus with foamy cytoplasm. We report a 16-year old female who presented with facial bone enlargement and also under follow-up with medical team to rule out b-Thalassemia. Histopathological examination of a biopsy for mandibular bone swelling showed presence of sheets of pseudo-Gaucher cells in the marrow spaces. The finding of these pseudo-Gaucher cells can be helpful to establish diagnosis and further management of this patient. Keywords: Pseudo-Gaucher cells, Thalassemia, mandible
AGE ESTIMATION IN CHILDREN BY MEASUREMENT OF OPEN APICES IN TOOTH ROOTS IN CHINESE POPULATION IN MALAYSIA: A RADIOLOGIC STUDY Renjith Pallivathukal George, Preethy Mary Donald, Sumanth Kumbargere Nagraj, Htoo Htoo Kyawe, Faculty of Dentistry, Melaka Manipal Medical College, Melaka, Malaysia Objective: Age estimation is used in the absence of accurate age data, forensic dentistry in human identification, paediatric endocrinology and in orthodontic treatment. This pilot study is done to test the accuracy of Cameriere’s age estimation method using European formula for Chinese children in Malaysia. Study Design: We evaluated the Orthopantomographs of 45 Chinese children (17 boys and 28 girls) aged between 5 to 18 years using Romexis 3.0.1R software. Dental age was calculated based on the European formula. Results: Pearson correlation between the chronological age and dental age was significant at 0.01 level with P value ¼ 0.909. Mean of difference of chronological age and dental age is 0.5547 years and the mean +/-2SD is 3.3234 to -2.214 years. Conclusions: Dental age calculated using the European formula showed high and significant correlation with the chronological age therefore the formula can be applied to the Chinese population in Malaysia. Keywords: Camriere, open apex, dental age, Chinese, European formula
ANGIOGENESIS OF ORAL LICHEN PLANUS: AN IMMUNOHISTOCHEMICAL STUDY Merva Soluk Tekkes¸in1, Alper Sinanoglu2, Vakur Olgac1, 1Department of Tumour Pathology, Institute of Oncology, Istanbul University, Istanbul, Turkey; 2Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Kocaeli University, Kocaeli, Turkey Objective: Oral lichen planus (OLP) is a relatively common disease and considered to be a chronic inflammatory disease with an autoimmune pathogenesis. Angiogenesis plays an important role in both physiological and pathologic conditions, including chronic inflammatory disorders. The aim of this immunohistochemical study was to investigate the role of angiogenesis in the pathogenesis of OLP.