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Effects of cold exposure on kidney toxicity of mercuric chloride
Poster
I1-3
Session
II
(6-7
March
1981)
A35
EFFECTS OF COLD EXPOSURE ON KIDNEY TOXICITY OF MERCURIC CHLORIDE.
V. BURGAT , J.P. BRAUN Toxicology Dep t 31076
and
TOULOUSE-CEDEX
, P. BENARD
, A.G. RICO
Biochemistry Dep t
, B. EGHBALI
Veterinary School
FRANCE.
The experiments were carried out in 3 groups of 20 male Sprague Dawley rats Weighing 250 to 350 g. They were given IP, O, 0.50, 1.0 mg Hg++/kg as HgCI 2 in I0 ml/kg saline
; for each dose i0 animals were exposed for 24h to a
I°C - cold stress and the I0 other were housed ~ 20°C.
In the 24h individual
urines the following parameters were measured = volume, Na, K, CI, urea, creatinine, G.G.T.
(EC 2322), P.A.L.
(EC 3131).
In control animals, the cold stress induced an hypotonic polyuria.
In
HgCI 2 treated animals at room temperature, kidney damage is demonstrated mainly by a dramatic increase of P.A.L. and G.G.T.
Cold stress decreases the mercury-
induced kidney damage as shown by relatively slight increase of enzymuria. This decrease of kidney toxicity is consistent with previous observations dealing with the effects of various diuretics.
I1-4
DISTRIBUTION AND NEPHROTOXICITY OF GLAFENINE IN RAT AND MOUSE. JEAN-PIERRE BRAUN, Andr~ Delhon, Patrick B~nard, Viviane Burgat and Andr~ Rico. Lab. de Biochlmle, Ecole Nationale V~t~rinalre, 31076 Toulouse Cedex, FRANCE." Macroscopic autoradiography in mouse demonstrated that 14C G1afenine i s rap i d l y distributed and eliminated : 30 mn after oral administration radioactivity is mainly in liver, kidney and digestive tract ; on the 4th hour, only a low radioactivity is observed in kidney and liver whereas radioactivity persists up to the 12th hours in the intestine. Oral dosage with 500 mg/kg induced an important increase of diuresis up to the 4th day ; Na +, K +, Creatlnine and Proteins daily excretions are lowered mainly on the first day. Daily excretion of urine Gamma-Glutamyl Transferase which is not inhibited by Glafenine is lowered for the two first days ; then it is enhanced on days 3 to 7 ; meanwhile serum urea and creatinine are increased whereas their clearances are decreased. Even if Slafenine Is rapidly cleared from the organism,
it determines both
early kidney functionnal disturbances and delayed tubule lesions.
G.P, 12/2---O
I1-3
Session
II
(6-7
March
1981)
A35
EFFECTS OF COLD EXPOSURE ON KIDNEY TOXICITY OF MERCURIC CHLORIDE.
V. BURGAT , J.P. BRAUN Toxicology Dep t 31076
and
TOULOUSE-CEDEX
, P. BENARD
, A.G. RICO
Biochemistry Dep t
, B. EGHBALI
Veterinary School
FRANCE.
The experiments were carried out in 3 groups of 20 male Sprague Dawley rats Weighing 250 to 350 g. They were given IP, O, 0.50, 1.0 mg Hg++/kg as HgCI 2 in I0 ml/kg saline
; for each dose i0 animals were exposed for 24h to a
I°C - cold stress and the I0 other were housed ~ 20°C.
In the 24h individual
urines the following parameters were measured = volume, Na, K, CI, urea, creatinine, G.G.T.
(EC 2322), P.A.L.
(EC 3131).
In control animals, the cold stress induced an hypotonic polyuria.
In
HgCI 2 treated animals at room temperature, kidney damage is demonstrated mainly by a dramatic increase of P.A.L. and G.G.T.
Cold stress decreases the mercury-
induced kidney damage as shown by relatively slight increase of enzymuria. This decrease of kidney toxicity is consistent with previous observations dealing with the effects of various diuretics.
I1-4
DISTRIBUTION AND NEPHROTOXICITY OF GLAFENINE IN RAT AND MOUSE. JEAN-PIERRE BRAUN, Andr~ Delhon, Patrick B~nard, Viviane Burgat and Andr~ Rico. Lab. de Biochlmle, Ecole Nationale V~t~rinalre, 31076 Toulouse Cedex, FRANCE." Macroscopic autoradiography in mouse demonstrated that 14C G1afenine i s rap i d l y distributed and eliminated : 30 mn after oral administration radioactivity is mainly in liver, kidney and digestive tract ; on the 4th hour, only a low radioactivity is observed in kidney and liver whereas radioactivity persists up to the 12th hours in the intestine. Oral dosage with 500 mg/kg induced an important increase of diuresis up to the 4th day ; Na +, K +, Creatlnine and Proteins daily excretions are lowered mainly on the first day. Daily excretion of urine Gamma-Glutamyl Transferase which is not inhibited by Glafenine is lowered for the two first days ; then it is enhanced on days 3 to 7 ; meanwhile serum urea and creatinine are increased whereas their clearances are decreased. Even if Slafenine Is rapidly cleared from the organism,
it determines both
early kidney functionnal disturbances and delayed tubule lesions.
G.P, 12/2---O