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Effects of exercise training on serum lipids and accumulation of triacylglycerols in rat liver
366
ANTIPYRINE, AMINOPYRINE AND SE-BILIRUBIN AS PROGNOSTIC INDICES OF F I V E Y E A R S U R V I V A L IN L I V E R C I R R H O S I S . B. R a s m u s s e n , M. D ~ s s i n g , H.E. P o u l s e n ~ N. T y g s t r u p . M e d i c a l d e p a r t m e n t A, R i g s h o s p i t a l e t , Copenhagen, Denmark.
T h e v a l u e of a n t i p y r i n e c l e a r a n c e (APC), 2hr a m i n o p y r i n e b r e a t h test (ABT), and c o n v e n t i o n a l b l o o d b i o c h e m i s t r y w a s d e t e r m i n e d in 44 p a t i e n t s w i t h h e p a t i c c i r r h o s i s . T w e l v e p a t i e n t s had p r i m a r y b i l i a r y c i r r h o s i s , 32 h a d a l c o h o l i c c i r r h o s i s . The p a t i e n t s w e r e e x a m i n e d c o n s e c u t i v e l y a f t e r adm i s s i o n to the d e p a r t m e n t . F i v e y e a r s l a t e r 14 p a t i e n t s had died, 4 w i t h p r i m a r y b l l i a r y c l r r h o s i s , i0 w i t h a l c o h o l i c c i r r h o s i s . D a t a w e r e a n a l y s e d by C o x ' s m o d e l for p r o p o r t i o n a l hazards. In o r d e r to r e d u c e the i n f l u e n c e of a f e w h i g h v a l u e s of s e - b i l l r u b i n (B), a l k a l i n e phosphatases (ALK) and a l a n i n e a m i n o t r a n s f e r a s e (ALA) the l o g a l i t h m of t h e s e v a r i a b l e s w a s used. U s i n g e a c h s i n g l e v a r i a b l e as a c o v a r i a t e the liver f u n c t i o n t e s t s w i t h s i g n i f i c a n t p r o g n o s t i c v a l u e w e r e B, APC, A B T and prothrombin index (PP), and t h o s e w i t h o u t s i g n i f i c a n t p r o g n o s t i c v a l u e w e r e A L A and ALK. F u r t h e r a n a l y s i s s h o w e d t h a t a m o d e l c o n t a i n i n g B and APC as c o v a r i a t e s had s i g n i f i c a n t p r o g n o s t i c value. T h e c o r r e l a t i o n (r) b e t w e e n B and A P C w a s 0.48. We c o n c l u d e t h a t s e - b i l l r u b l n has s i g n i f i c a n t p r o g n o s t i c v a l u e and t h a t f u r t h e r p r o g n o s t i c i n f o r m a t i o n can be o b t a i n e d by e v a l u a t i n g antipyrine clearance.
367
EFFECTS OF EXERCISE TRAINING ON SERUM LIPIDS AND ACCUMULATION OF TRIACYLGLYCEROLS IN RAT LIVER A. Riutto, J. Vuori*, V. Vihko Research Institute for Physical Activity and Health, University Campus, SF-40700 Jyv~skyl~ and Department of Cell Biology, University of Jyv~skyl~, SF-ZK)IOO Jyv~L~kyl~ and *Sports Clinic of the Deaconess Institute of Oulu, SF-90100 Oulu, Finland
Our studies on mice have shown that i) during 8 h rtmning triacylglycerols (TG) accumulate in the hepatocytes in proportion to thelength of the exertion, and that 2) prior running training decreases the exertion-induced TG accumulation, which does not occur after i0 d training. In this study we compared liver TG content and serum lipid levels in prior-trained exhausted and in sedentary exhausted male Wistar rats, aged 4 months. Twelve rats were subjected to daily swimming training 37-39 times (74-?9 h). Six of the trained and six of the sedentary rats were made to run on a motor-driven treadmill at 20 m/rain with 6 ° uphill tracks until exhausted. Training did not affected liver TG. Exhaustion increased liver TG by i07 % in the controls (p <0.05) but not in the trained rats. Training and/or exhaustion did not affect S-HDL-cholesterol, S-f~- or S-pre-f3-1ipoproteins. Exhaustion decreased S - ~ lipoproteins in the control rats (p< 0.01), only. Post-exercise S-FFA and S-f~-hydroxybutyrate concentrations were increased in the control and in the trained groups (p <0.01). A slight training effect (a decrease) occurred in the post-exercise S-FFA (p < 0.05). Training decreased S-cholesterol and S-TG (p <0.05), while exhaustion decreased S-TG in the trained group (p < 0.05), only. The results show that swimming as well as running training prevents exercise-induced fatty liver and has a decreasing effect on S-FFA, S-TG and S-cholesterol in rats.
$189
ANTIPYRINE, AMINOPYRINE AND SE-BILIRUBIN AS PROGNOSTIC INDICES OF F I V E Y E A R S U R V I V A L IN L I V E R C I R R H O S I S . B. R a s m u s s e n , M. D ~ s s i n g , H.E. P o u l s e n ~ N. T y g s t r u p . M e d i c a l d e p a r t m e n t A, R i g s h o s p i t a l e t , Copenhagen, Denmark.
T h e v a l u e of a n t i p y r i n e c l e a r a n c e (APC), 2hr a m i n o p y r i n e b r e a t h test (ABT), and c o n v e n t i o n a l b l o o d b i o c h e m i s t r y w a s d e t e r m i n e d in 44 p a t i e n t s w i t h h e p a t i c c i r r h o s i s . T w e l v e p a t i e n t s had p r i m a r y b i l i a r y c i r r h o s i s , 32 h a d a l c o h o l i c c i r r h o s i s . The p a t i e n t s w e r e e x a m i n e d c o n s e c u t i v e l y a f t e r adm i s s i o n to the d e p a r t m e n t . F i v e y e a r s l a t e r 14 p a t i e n t s had died, 4 w i t h p r i m a r y b l l i a r y c l r r h o s i s , i0 w i t h a l c o h o l i c c i r r h o s i s . D a t a w e r e a n a l y s e d by C o x ' s m o d e l for p r o p o r t i o n a l hazards. In o r d e r to r e d u c e the i n f l u e n c e of a f e w h i g h v a l u e s of s e - b i l l r u b i n (B), a l k a l i n e phosphatases (ALK) and a l a n i n e a m i n o t r a n s f e r a s e (ALA) the l o g a l i t h m of t h e s e v a r i a b l e s w a s used. U s i n g e a c h s i n g l e v a r i a b l e as a c o v a r i a t e the liver f u n c t i o n t e s t s w i t h s i g n i f i c a n t p r o g n o s t i c v a l u e w e r e B, APC, A B T and prothrombin index (PP), and t h o s e w i t h o u t s i g n i f i c a n t p r o g n o s t i c v a l u e w e r e A L A and ALK. F u r t h e r a n a l y s i s s h o w e d t h a t a m o d e l c o n t a i n i n g B and APC as c o v a r i a t e s had s i g n i f i c a n t p r o g n o s t i c value. T h e c o r r e l a t i o n (r) b e t w e e n B and A P C w a s 0.48. We c o n c l u d e t h a t s e - b i l l r u b l n has s i g n i f i c a n t p r o g n o s t i c v a l u e and t h a t f u r t h e r p r o g n o s t i c i n f o r m a t i o n can be o b t a i n e d by e v a l u a t i n g antipyrine clearance.
367
EFFECTS OF EXERCISE TRAINING ON SERUM LIPIDS AND ACCUMULATION OF TRIACYLGLYCEROLS IN RAT LIVER A. Riutto, J. Vuori*, V. Vihko Research Institute for Physical Activity and Health, University Campus, SF-40700 Jyv~skyl~ and Department of Cell Biology, University of Jyv~skyl~, SF-ZK)IOO Jyv~L~kyl~ and *Sports Clinic of the Deaconess Institute of Oulu, SF-90100 Oulu, Finland
Our studies on mice have shown that i) during 8 h rtmning triacylglycerols (TG) accumulate in the hepatocytes in proportion to thelength of the exertion, and that 2) prior running training decreases the exertion-induced TG accumulation, which does not occur after i0 d training. In this study we compared liver TG content and serum lipid levels in prior-trained exhausted and in sedentary exhausted male Wistar rats, aged 4 months. Twelve rats were subjected to daily swimming training 37-39 times (74-?9 h). Six of the trained and six of the sedentary rats were made to run on a motor-driven treadmill at 20 m/rain with 6 ° uphill tracks until exhausted. Training did not affected liver TG. Exhaustion increased liver TG by i07 % in the controls (p <0.05) but not in the trained rats. Training and/or exhaustion did not affect S-HDL-cholesterol, S-f~- or S-pre-f3-1ipoproteins. Exhaustion decreased S - ~ lipoproteins in the control rats (p< 0.01), only. Post-exercise S-FFA and S-f~-hydroxybutyrate concentrations were increased in the control and in the trained groups (p <0.01). A slight training effect (a decrease) occurred in the post-exercise S-FFA (p < 0.05). Training decreased S-cholesterol and S-TG (p <0.05), while exhaustion decreased S-TG in the trained group (p < 0.05), only. The results show that swimming as well as running training prevents exercise-induced fatty liver and has a decreasing effect on S-FFA, S-TG and S-cholesterol in rats.
$189