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Effects of ginseng on lipid profile and oxidative stress in dyslipidemic patients
S344
Abstracts
extract as reducing power showed low inhibition compared to ascorbic acid, α-tocopherol, and BHT with values of 53.5 ± 1.51, 93.3 ± 1.13%, 83.7 ± 1.65%, and 93.1 ± 3.46% at a concentration of 100 μg/mL respectively. Even though the antioxidant activity of the methanolic extract was moderate as compared to the positive controls but still it could be applied as a source of natural antioxidant in food, pharmaceutical and cosmetic industries and its biological activities could be due to the presence of phenolics and flavonoids as bioactive compounds present in the seed. Keywords: Alhagi maurorum, Antioxidant activity, Phenolic, Flavonoid, Bioactive compounds
doi:10.1016/j.clinbiochem.2011.08.856
Poster – [A-10-851-1] Effects of ginseng on lipid profile and oxidative stress in dyslipidemic patients Manavifar Mortezaa, Fatehi Hedayatollahb, Hasanzadeh Dalooei Mahdib, Ghayour Mobarhan Majidc, Akhlaghi Saeedd, Amini Marale, Tavallaei Shimaf a Quaem Hospital, Ahmadabad St., Mashhad, Iran b Cardiovascular Research Centre, Quaem Hospital, Mashhad, Iran c Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran d Faculty of Biostatistics in Health Sciences School, Mashhad University of Medical Science, Mashhad, Iran e Young Researchers Club, Mashhad Islamic Azad University, Mashhad Branch, Mashhad, Iran f Bou-Ali Research Centre, Mashhad, Iran E-mail addresses: [email protected] (M. Morteza), [email protected] (F. Hedayatollah), [email protected] (H.D. Mahdi), [email protected] (G.M. Majid), [email protected] (A. Saeed), [email protected] (A. Maral), [email protected] (T. Shima) Introduction: Dyslipidemia is one of the major risk factors for coronary heart disease (CHD). In recent years “novel atherosclerotic risk factors” also have been denoted like hsCRP and oxidative stress, which can become the target of preventive measures. Some studies have reported positive effect of ginseng on cardiovascular risk factors; although, there have been few studies, especially randomised trials, about its effect on human lipid profile, hsCRP and oxidative stress and the results are inconsistent. This study was designed to determine the effect of ginseng capsules on serum lipids and also on hsCRP and prooxidant anti-oxidant balance (PAB). Materials and methods: This was a double blind randomised clinical trial. Forty patients who were eligible for primary prevention of hyperlipidemia and met the rule in criteria for our study were selected and randomly assigned to case or control group and took ginseng capsules 500 mg twice daily for 8 weeks in case group, or placebo in control group, in addition to life style modification (diet and exercise) in both groups. The results were analysed with Repeated Measures ANOVA. Results: Serum levels of Total cholesterol (TC), LDL cholesterol (LDL-c), Triglyceride(TG), and Total cholesterol to HDL (TC/HDL) ratio were significantly decreased in both groups. But there was no statistically important difference between the two groups for Total cholesterol (p = 0.210), LDL-c (p = 0.245), HDL-c (p = 0.827), TC/HDL ratio (p = 0.427), hsCRP (p = 0.880) and PAB (p = 0.545). Discussion: Nutrition and exercise have positive effects on lipid profile, but ginseng does not improve serum levels of Total cholesterol, LDLc, HDLc, Trigliceride, hsCRP and anti-oxidants.
Keywords: Ginseng, Lipid profile, hsCRP, Pro-oxidant anti-oxidant balance doi:10.1016/j.clinbiochem.2011.08.857
Poster — [A-10-875-1] Cloning of tropinone reductase-II (TR-II) gene in Agrobacterium tumefacience Mitra Parsaab, Ghasemali Garoosia, Raheem Haddada a Department of Biotechnology, International University of Imam Khomein, Iran b Department of Plant Biology, Institute of Applied Science, Shahid Beheshti Univercity, Tehran, Iran E-mail addresses: [email protected] (M. Parsa), [email protected] (G. Garoosi), [email protected] (R. Haddad) Introduction: Two tropane alkaloids hyoscine (scopolamine) and hyoscyamine are the valuable medicines, which are extracted from solanaceae plant family such as Hyoscyamus niger. These alkalioids are used for pertussis and chronic bronchitis remedy because of their anti tension pain effects and impression on parasampatic nervous system. In tropane alkaloids synthesis pathway, tropinone as intermediate subestrate is converted to hyoscyamine and hyoscyne by tropinone reductase-I (TR-I), whereas tropinone reductase-II (TR-II) converts it compitantly to calystegines, a nontropand alkaloid. Material and method: In this research to reduce synthesis of TR-II comparative to TR-I enzyme, tr-II gene from Hyoscyamus niger was cloned in anti-sense direction in binary vector and transferred to Agrobacterium tumefacience. In this way, young roots from germinated Hyoscyamus niger seeds on B5 medium were propagated in same liquid medium supplemented with 1 μM/L IBA. Following total RNA isolation from proliferated roots, cDNA of interest gene was made and after specific PCR amplification was ligated into pBluescript phagmid. Rcombinant phagmid was transferred to E. coli, strain DH5α. Recombinant vector, following extraction from bacteria was confirmed with 3 methods, digestion, PCR and DNA sequencing. Result and conclusion: cDNA sequencing and deduced amino acid sequence of target gene from Iranian native Hyoscyamus niger indicated that it has respectively 99% and 100% similarity with recorded NCBI one's. Subsequently, interest gene was cloned in binary vector pBI121 in anti-sense direction and recombinant vector introduced into Agrobacterium tumefacience strain GV3101. Keywords: Cloning, Hyoscyamine, tr-II gene, Hyoscyamus niger, Tropinone reductase-II doi:10.1016/j.clinbiochem.2011.08.858
Poster — [A-10-894-1] Dose asafoetida (Ferula assafoetida oleo-gum-resin) extract has relaxant effects on guinea-pig tracheal chains Zahra Gholamnezhada, Mohammad Hossein Boskabadya, Mehrdad Iranshahib, Goltaj Bayramia a Dept. of Physiology and Pharmaceutical Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b Department of Pharmacognosy, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (Z. Gholamnezhad), [email protected] (M.H. Boskabady), [email protected] (M. Iranshahi), [email protected] (G. Bayrami) Introduction: Ferula assafoetida oleo-gum-resin (asafoetida) has been used as a folk phytomedicine for centuries in asthma treatment
Abstracts
extract as reducing power showed low inhibition compared to ascorbic acid, α-tocopherol, and BHT with values of 53.5 ± 1.51, 93.3 ± 1.13%, 83.7 ± 1.65%, and 93.1 ± 3.46% at a concentration of 100 μg/mL respectively. Even though the antioxidant activity of the methanolic extract was moderate as compared to the positive controls but still it could be applied as a source of natural antioxidant in food, pharmaceutical and cosmetic industries and its biological activities could be due to the presence of phenolics and flavonoids as bioactive compounds present in the seed. Keywords: Alhagi maurorum, Antioxidant activity, Phenolic, Flavonoid, Bioactive compounds
doi:10.1016/j.clinbiochem.2011.08.856
Poster – [A-10-851-1] Effects of ginseng on lipid profile and oxidative stress in dyslipidemic patients Manavifar Mortezaa, Fatehi Hedayatollahb, Hasanzadeh Dalooei Mahdib, Ghayour Mobarhan Majidc, Akhlaghi Saeedd, Amini Marale, Tavallaei Shimaf a Quaem Hospital, Ahmadabad St., Mashhad, Iran b Cardiovascular Research Centre, Quaem Hospital, Mashhad, Iran c Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran d Faculty of Biostatistics in Health Sciences School, Mashhad University of Medical Science, Mashhad, Iran e Young Researchers Club, Mashhad Islamic Azad University, Mashhad Branch, Mashhad, Iran f Bou-Ali Research Centre, Mashhad, Iran E-mail addresses: [email protected] (M. Morteza), [email protected] (F. Hedayatollah), [email protected] (H.D. Mahdi), [email protected] (G.M. Majid), [email protected] (A. Saeed), [email protected] (A. Maral), [email protected] (T. Shima) Introduction: Dyslipidemia is one of the major risk factors for coronary heart disease (CHD). In recent years “novel atherosclerotic risk factors” also have been denoted like hsCRP and oxidative stress, which can become the target of preventive measures. Some studies have reported positive effect of ginseng on cardiovascular risk factors; although, there have been few studies, especially randomised trials, about its effect on human lipid profile, hsCRP and oxidative stress and the results are inconsistent. This study was designed to determine the effect of ginseng capsules on serum lipids and also on hsCRP and prooxidant anti-oxidant balance (PAB). Materials and methods: This was a double blind randomised clinical trial. Forty patients who were eligible for primary prevention of hyperlipidemia and met the rule in criteria for our study were selected and randomly assigned to case or control group and took ginseng capsules 500 mg twice daily for 8 weeks in case group, or placebo in control group, in addition to life style modification (diet and exercise) in both groups. The results were analysed with Repeated Measures ANOVA. Results: Serum levels of Total cholesterol (TC), LDL cholesterol (LDL-c), Triglyceride(TG), and Total cholesterol to HDL (TC/HDL) ratio were significantly decreased in both groups. But there was no statistically important difference between the two groups for Total cholesterol (p = 0.210), LDL-c (p = 0.245), HDL-c (p = 0.827), TC/HDL ratio (p = 0.427), hsCRP (p = 0.880) and PAB (p = 0.545). Discussion: Nutrition and exercise have positive effects on lipid profile, but ginseng does not improve serum levels of Total cholesterol, LDLc, HDLc, Trigliceride, hsCRP and anti-oxidants.
Keywords: Ginseng, Lipid profile, hsCRP, Pro-oxidant anti-oxidant balance doi:10.1016/j.clinbiochem.2011.08.857
Poster — [A-10-875-1] Cloning of tropinone reductase-II (TR-II) gene in Agrobacterium tumefacience Mitra Parsaab, Ghasemali Garoosia, Raheem Haddada a Department of Biotechnology, International University of Imam Khomein, Iran b Department of Plant Biology, Institute of Applied Science, Shahid Beheshti Univercity, Tehran, Iran E-mail addresses: [email protected] (M. Parsa), [email protected] (G. Garoosi), [email protected] (R. Haddad) Introduction: Two tropane alkaloids hyoscine (scopolamine) and hyoscyamine are the valuable medicines, which are extracted from solanaceae plant family such as Hyoscyamus niger. These alkalioids are used for pertussis and chronic bronchitis remedy because of their anti tension pain effects and impression on parasampatic nervous system. In tropane alkaloids synthesis pathway, tropinone as intermediate subestrate is converted to hyoscyamine and hyoscyne by tropinone reductase-I (TR-I), whereas tropinone reductase-II (TR-II) converts it compitantly to calystegines, a nontropand alkaloid. Material and method: In this research to reduce synthesis of TR-II comparative to TR-I enzyme, tr-II gene from Hyoscyamus niger was cloned in anti-sense direction in binary vector and transferred to Agrobacterium tumefacience. In this way, young roots from germinated Hyoscyamus niger seeds on B5 medium were propagated in same liquid medium supplemented with 1 μM/L IBA. Following total RNA isolation from proliferated roots, cDNA of interest gene was made and after specific PCR amplification was ligated into pBluescript phagmid. Rcombinant phagmid was transferred to E. coli, strain DH5α. Recombinant vector, following extraction from bacteria was confirmed with 3 methods, digestion, PCR and DNA sequencing. Result and conclusion: cDNA sequencing and deduced amino acid sequence of target gene from Iranian native Hyoscyamus niger indicated that it has respectively 99% and 100% similarity with recorded NCBI one's. Subsequently, interest gene was cloned in binary vector pBI121 in anti-sense direction and recombinant vector introduced into Agrobacterium tumefacience strain GV3101. Keywords: Cloning, Hyoscyamine, tr-II gene, Hyoscyamus niger, Tropinone reductase-II doi:10.1016/j.clinbiochem.2011.08.858
Poster — [A-10-894-1] Dose asafoetida (Ferula assafoetida oleo-gum-resin) extract has relaxant effects on guinea-pig tracheal chains Zahra Gholamnezhada, Mohammad Hossein Boskabadya, Mehrdad Iranshahib, Goltaj Bayramia a Dept. of Physiology and Pharmaceutical Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b Department of Pharmacognosy, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (Z. Gholamnezhad), [email protected] (M.H. Boskabady), [email protected] (M. Iranshahi), [email protected] (G. Bayrami) Introduction: Ferula assafoetida oleo-gum-resin (asafoetida) has been used as a folk phytomedicine for centuries in asthma treatment