Effects of growth hormone on the chick embryo

L1=e Sciences Ao . ûaited States .

8, pp" 624-628, 1963.

Per~mon Press, Inc .

Printed in the

aH0lTS HOHI~E ~ SBS C.HICC ~[8RZ0~ L. D~~ovaso - a. aindioi Istituto di lisiologia, IIniversità di Srleste, l1aa1is (BeoeiTed 20 Jbae 1963) Ii: is well estaälis~hed that the rasoval as the hJpop~sis or the adiiaistratioa of sasr~totropio horaone,during poet-natal life, brings about suiting cheats u the growth at the sYeleton ss well u oa the weight - of the boàf os sage o! its organs : but u tss u the prenatal developsuent is oonoeraed, the i~portaaoe o! the hTpoph sis i~as soaroe7,T bees treated . It seea.ed interesting, therefore, to observe the etYeots of the growth horaone on the dsvelopstieat o? heart, spleen sad liver (i .e . of organs of àitterent estbs?ogeaetio origin and tta
Ot

She iajeotioaas were aaàe is twelve-day~old ss~os into the oarionrallantola usabraae . 120 eggs were iajeoted with ß II. (3~ans) os bovine scaeato troplo hos~one (supplied ~ Choy) ;the eggs of the control grasp (120) were injected with the diluting Tolune (0.2 al) o! the saline solution. One week later,ths cabs-~os were eauinsd.shs heart liner sad spleen were oareth] .],7 dissected . She content is BUl sad alV ws~s cheated by the aethoàs of aisohe (t ) and lle~bauad (2 ) respectively; the ooaateat of protein nitrogen was also auasnred . "+r nentiansd abone,aar observations were ~ad~ on 19-daT-old ~ Shis wort ess enpported 8lcerohe (C .w.B.), Boaa.

ia

part ~ Co~nsiglio alssionale

delle

~s oF a~ra ~o~sE ae ~ c~cg ~0

Ho. 8

625

es
I

and

II.

8.11 .49;~a,~d

She >aa boày~wei8ht was renal to be :$ with the gs~owth hos~one g. 11 .82 .

She àata point out railnre or the growth bosons is rsspeot

to the growing or the bow.

Oa the ooatrary,it was round that in the oontrol gron~p

the

wean weights or the spleen (8.0.0069) and hart (8.0.1153) w re clearly interior to the treated .w+~~~ (8.0.010 anà g. 0.1421

respea~tive].y) .~ia eâeet was 3taöag a !!~r as liver is eaaoerned,lbs weight

or

this organ beia8 sinilas lathe aaisaL ore the two grv~s .

She ~l and ~Il content la the spleen sod heat of the treated

ahiets was round to be increased as ooapared to

the

controls,

mile the anoleie soils eontent is the liver appeared not to be aodiried b8 the hos~one .

Disausion

Jocordin8 to Esieh dal. (3), oa~tiraed ~ aa~e or >;s (4), the iarlneaoe or the growth hos~aaa on the developaent or the boa? it vrrT poor in the chi.at etbr~o. She results

or

the present wor]c, elarly pout aait

that

this horaaae is very errioieat is stinnlstiag the eabs~oaic growth or both the spleen and heart bat, a the other hand, it wens to have ao sedan oar the denlopaeat or bogy and ].iva .

She aeohanisa bf which the saeatotropio bosons ay arrest

the growth or the viscera is not the object

of this

iavrstig~

anon ; it wens interesting, however, to note how the respaaias

or

the viscera or the ahiot are is percentage

rres< one another. Shin obsernatiaa~ agrees with previcas

quite

dirrerent

researehes (5)

as

the i>ypopbyseetamd.sed rat treated with sasjatotropio hos~one . Growth or tissue, associated with

an increase

or

all

cellular coastitnents mJ be szpeoted to require as incraad synthesis or mieleoproteins and related eas~annds.

626

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In thi~ worh wr baw obsernd that the inorasao is the weigitt o! the oploos and heart wso ~ollowed ~ an inoreaaed oontent a~ nonolei,o aoids (both nU and Bâl) while the li~r, the sise o~i whiah s~aiaed nrra!leoted, ahowed no sodi~ioatia~nai to its noraal aaaaat oi Dl~l and HHI. ~tereaoer 7) Z . Dieohe - =i]crooheaie . ~, 4 (193~) 2 ) 1f. Ye~ban~ - Z .Pb~iol. ~tet. ~, 117 (1939) 3) â.Y. Hsieh, l.Y. 11hnE aad B.S . 8ina~ttLsl-~g.~,298 (1952) 4) L.D~~ooaso, S .Polaaio and P.Zatti - 7s~ori de1l~Istitn~ di orda e Qhiaica Biolsaioa - IIai~rsità di rrieste (1959) ~, 151 (1949) 5) ]E.E.Sia~san, 8.]Lilnans aad O .H.Id -