- Email: [email protected]
Effects of magnesium and calcium cations on biofilm formation by Sphingomonas paucimobilis from an industrial environment
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Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
the predominant hydrogen producer present in the fermentation broth at the optimum condition was Megaspheara sp.
using RAPD-PCR technique can be provide fast and more useful differentiation at the strain level, comparing to the numerical analysis of protein profiles.
http://dx.doi.org/10.1016/j.copbio.2013.05.182 http://dx.doi.org/10.1016/j.copbio.2013.05.184 Improving the quality of hydrogen sulfide contaminated biogas by bioscrubber Jitrutai Tosati, Siriwat Jinsiriwanit Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand E-mail address: [email protected] (S. Jinsiriwanit). The objective of this study is to develop a process to remove hydrogen sulfide (H2 S) from biogas by bioscrubber. The laboratory scale bioscrubber system in this study was a 0.05 L absorption tray tower with a 1.5 L biological oxidation reactor inoculated with sulfur oxidizer from a local waste water treatment plant. The performances of biosrubber were studied in different inlet H2 S concentrations and different empty bed residence times (EBRT). H2 S concentrations were varied from 407 to 4153 ppmv at EBRT of 63 s corresponded to inlet H2 S loading rate of 32–326 g H2 S m−3 h−1 . The maximum removal efficiency of the bioscrubber was 80% at 37 g H2 S m−3 h−1 inlet loading rate. The maximum elimination capacity of the bioscrubber was 160 g H2 S m m−3 h−1 at an inlet loading rate of 320 g H2 S m−3 h−1 . The maximum removal efficiency of 1000 ppmv H2 S biogas was 75, 89, 93 and 99% at EBRT of 63, 79, 90 and 105 s respectively. The bioscrubber also had little effects on carbon dioxide removal. About 2% of CO2 was also removed from the biogas stream while the CO2 concentration of untreated biogas was around 10–12%. Overall, the bioscrubber may be another environmental friendly mean to improve the quality of H2 S contaminated biogas without the necessity to use hazardous chemicals.
Effects of magnesium and calcium cations on biofilm formation by Sphingomonas paucimobilis from an industrial environment Nur Ceyhan Guvensen 1 , Serdar Demir 2 , Guven Ozdemir 3 1
Department of Biology, Faculty of Science, Mu˘gla Sıtkı Koc¸man University, Mu˘gla, Turkey 2 Department of Statistics, Faculty of Science, Mu˘ gla Sıtkı Koc¸man University, Mu˘gla, Turkey 3 Department of Biology, Faculty of Science, Ege University, I˙ zmir, Turkey E-mail address: [email protected] (N.C. Guvensen).
Molecular characterization of Escherichia coli strains isolated from sea water and black mussel of Sinop Peninsula, Turkey
Bacterial biofilms may form on all surface-associated natural and many industrial environments. Biofilm formation requires particular notice due to its associated risks for human health and its impact on environmental contamination and pollution. In this work, we investigated the effects of Mg2+ and Ca2+ ions on biofilm formation by Sphingomonas paucimobilis from an industrial environment. The biofilm formation on coupons within nutrient broth medium was significantly enhanced after addition of 0, 100, 250 and 500 M Mg2+ . Similarly, the addition of Ca2+ caused a significant increase in S. paucimobilis biofilm formation when the above concentration levels for Ca2+ were tested. In contrast, the same concentrations of these ions had no effect on growth of free-living (planktonic) S. paucimobilis cells in the medium. Hence, Mg2+ and Ca2+ increased the biofilm formation as adherent-cells on the coupons. Both ion types were significantly effective on S. paucimobilis biofilm formation, particularly at 100 and 250 M (P ≤ 0.05). These firstly reported data for S. paucimobilis biofilms are important in the elucidation of the roles of divalent cations, such as Mg2+ and Ca2+ , in bacterial adhesion to the environmental surfaces for biofilm formation, and prevention of environmental contamination by this bacterium.
Cumhur Avs¸ar, Ismet Berber
http://dx.doi.org/10.1016/j.copbio.2013.05.185
Department of Biology, Faculty of Arts and Sciences, Sinop University, Sinop, Turkey E-mail address: [email protected] (C. Avs¸ar).
Phosphorus dynamics in a grassland ecosystem after mineral fertilization
http://dx.doi.org/10.1016/j.copbio.2013.05.183
Escherichia coli is one of the most reliable indicator of fecal pollution in seawater and seafood safety. Therefore, the identification of E. coli is important at the species or strain level. The purpose of the study was to characterize a total of 41 E. coli strains isolated from sea water and mussel according to protein and DNA profiles obtained by using SDS-PAGE and RAPD-PCR methods. Wholecell and extracellular proteins were extracted from each strain according to the modified methods of Berber et al. (2010). Polyacrylamide gel electrophoresis of proteins was performed. Genomic DNA extraction was carried out. Amplified DNA in PCR was subjected to electrophoresis on a 1% agarose gel and stained with ethidium bromide for photography under UV illuminator. Hierarchical cluster analysis of protein and DNA profiles was performed by using UPGMA method based on Jaccard similarity coefficient. The findings showed that the SDS-PAGE of whole-cell and extracellular protein was to good typing tool for the differentiation of E. coli strains at species level, not strain level. In addition, it was pointed out that extracellular protein profiles provided better distinction than whole cell protein profiles. On the contrary, the study supposed that the numerical analysis of DNA profiles produced by
Ciprian Stroia 1 , Adina Berbecea 2 , Marius Stroia 2 , Florin Crista 2 , Narcis Baghina 3 , Ioan Gaica 3 1
Department Biology, Banat’s University of Agricultural Science and Veterinary Medicine From Timisoara, Timisoara, Romania 2 Department Soil Science, Banat’s University of Agricultural Science and Veterinary Medicine from Timisoara, Timisoara, Romania 3 Department Environmental Engeeniring and Sustainable Developement, Banat’s University of Agricultural Science and Veterinary Medicine From Timisoara, Timisoara, Romania E-mail address: [email protected] (C. Stroia). This paper aims to improve the functioning of nutrient cycle and especially phosphorus in the context of sustainable agriculture for seeking a realization of environmental resources taking into account economic and environmental constraints. We propose evaluating the bioavailability of phosphorus in a grassland ecosystem located in the village Borlova, Caras-Severin. Were applied fertilizer nitrogen and phosphorus as follows: N0P0, N1P0, N0P1 and N1P1, where N0P0 unfertilized control, N1 = 150 kg N/ha was applied split and P1 = 50 kg P2 O5 /ha. P was applied once before
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
the predominant hydrogen producer present in the fermentation broth at the optimum condition was Megaspheara sp.
using RAPD-PCR technique can be provide fast and more useful differentiation at the strain level, comparing to the numerical analysis of protein profiles.
http://dx.doi.org/10.1016/j.copbio.2013.05.182 http://dx.doi.org/10.1016/j.copbio.2013.05.184 Improving the quality of hydrogen sulfide contaminated biogas by bioscrubber Jitrutai Tosati, Siriwat Jinsiriwanit Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand E-mail address: [email protected] (S. Jinsiriwanit). The objective of this study is to develop a process to remove hydrogen sulfide (H2 S) from biogas by bioscrubber. The laboratory scale bioscrubber system in this study was a 0.05 L absorption tray tower with a 1.5 L biological oxidation reactor inoculated with sulfur oxidizer from a local waste water treatment plant. The performances of biosrubber were studied in different inlet H2 S concentrations and different empty bed residence times (EBRT). H2 S concentrations were varied from 407 to 4153 ppmv at EBRT of 63 s corresponded to inlet H2 S loading rate of 32–326 g H2 S m−3 h−1 . The maximum removal efficiency of the bioscrubber was 80% at 37 g H2 S m−3 h−1 inlet loading rate. The maximum elimination capacity of the bioscrubber was 160 g H2 S m m−3 h−1 at an inlet loading rate of 320 g H2 S m−3 h−1 . The maximum removal efficiency of 1000 ppmv H2 S biogas was 75, 89, 93 and 99% at EBRT of 63, 79, 90 and 105 s respectively. The bioscrubber also had little effects on carbon dioxide removal. About 2% of CO2 was also removed from the biogas stream while the CO2 concentration of untreated biogas was around 10–12%. Overall, the bioscrubber may be another environmental friendly mean to improve the quality of H2 S contaminated biogas without the necessity to use hazardous chemicals.
Effects of magnesium and calcium cations on biofilm formation by Sphingomonas paucimobilis from an industrial environment Nur Ceyhan Guvensen 1 , Serdar Demir 2 , Guven Ozdemir 3 1
Department of Biology, Faculty of Science, Mu˘gla Sıtkı Koc¸man University, Mu˘gla, Turkey 2 Department of Statistics, Faculty of Science, Mu˘ gla Sıtkı Koc¸man University, Mu˘gla, Turkey 3 Department of Biology, Faculty of Science, Ege University, I˙ zmir, Turkey E-mail address: [email protected] (N.C. Guvensen).
Molecular characterization of Escherichia coli strains isolated from sea water and black mussel of Sinop Peninsula, Turkey
Bacterial biofilms may form on all surface-associated natural and many industrial environments. Biofilm formation requires particular notice due to its associated risks for human health and its impact on environmental contamination and pollution. In this work, we investigated the effects of Mg2+ and Ca2+ ions on biofilm formation by Sphingomonas paucimobilis from an industrial environment. The biofilm formation on coupons within nutrient broth medium was significantly enhanced after addition of 0, 100, 250 and 500 M Mg2+ . Similarly, the addition of Ca2+ caused a significant increase in S. paucimobilis biofilm formation when the above concentration levels for Ca2+ were tested. In contrast, the same concentrations of these ions had no effect on growth of free-living (planktonic) S. paucimobilis cells in the medium. Hence, Mg2+ and Ca2+ increased the biofilm formation as adherent-cells on the coupons. Both ion types were significantly effective on S. paucimobilis biofilm formation, particularly at 100 and 250 M (P ≤ 0.05). These firstly reported data for S. paucimobilis biofilms are important in the elucidation of the roles of divalent cations, such as Mg2+ and Ca2+ , in bacterial adhesion to the environmental surfaces for biofilm formation, and prevention of environmental contamination by this bacterium.
Cumhur Avs¸ar, Ismet Berber
http://dx.doi.org/10.1016/j.copbio.2013.05.185
Department of Biology, Faculty of Arts and Sciences, Sinop University, Sinop, Turkey E-mail address: [email protected] (C. Avs¸ar).
Phosphorus dynamics in a grassland ecosystem after mineral fertilization
http://dx.doi.org/10.1016/j.copbio.2013.05.183
Escherichia coli is one of the most reliable indicator of fecal pollution in seawater and seafood safety. Therefore, the identification of E. coli is important at the species or strain level. The purpose of the study was to characterize a total of 41 E. coli strains isolated from sea water and mussel according to protein and DNA profiles obtained by using SDS-PAGE and RAPD-PCR methods. Wholecell and extracellular proteins were extracted from each strain according to the modified methods of Berber et al. (2010). Polyacrylamide gel electrophoresis of proteins was performed. Genomic DNA extraction was carried out. Amplified DNA in PCR was subjected to electrophoresis on a 1% agarose gel and stained with ethidium bromide for photography under UV illuminator. Hierarchical cluster analysis of protein and DNA profiles was performed by using UPGMA method based on Jaccard similarity coefficient. The findings showed that the SDS-PAGE of whole-cell and extracellular protein was to good typing tool for the differentiation of E. coli strains at species level, not strain level. In addition, it was pointed out that extracellular protein profiles provided better distinction than whole cell protein profiles. On the contrary, the study supposed that the numerical analysis of DNA profiles produced by
Ciprian Stroia 1 , Adina Berbecea 2 , Marius Stroia 2 , Florin Crista 2 , Narcis Baghina 3 , Ioan Gaica 3 1
Department Biology, Banat’s University of Agricultural Science and Veterinary Medicine From Timisoara, Timisoara, Romania 2 Department Soil Science, Banat’s University of Agricultural Science and Veterinary Medicine from Timisoara, Timisoara, Romania 3 Department Environmental Engeeniring and Sustainable Developement, Banat’s University of Agricultural Science and Veterinary Medicine From Timisoara, Timisoara, Romania E-mail address: [email protected] (C. Stroia). This paper aims to improve the functioning of nutrient cycle and especially phosphorus in the context of sustainable agriculture for seeking a realization of environmental resources taking into account economic and environmental constraints. We propose evaluating the bioavailability of phosphorus in a grassland ecosystem located in the village Borlova, Caras-Severin. Were applied fertilizer nitrogen and phosphorus as follows: N0P0, N1P0, N0P1 and N1P1, where N0P0 unfertilized control, N1 = 150 kg N/ha was applied split and P1 = 50 kg P2 O5 /ha. P was applied once before