- Email: [email protected]
Effects of metal salts at sub-toxic concentrations
20
Abstracts / European Journal of Integrative Medicine 8S (2016) 1–66
46
47
Effects of metal salts at sub-toxic concentrations
Impact of neural therapy (NT) as an integrative therapy in head and neck
Carmen Steinborn ∗ , Christoph Diegel, Manuel Garcia-Käufer, Carsten Gründemann, Roman Huber
Fischer Gregor 1,∗ , Jünger Heinz 1,2
Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Breisacher Str. 115B, 79111 Freiburg, Germany E-mail address: [email protected] (C. Steinborn). Introduction: Because different metals are used in complementary medicine for the treatment of diseases related to a dysfunction of the immune system, this study aimed at determining the immunomodulatory potential of Pb(NO3 )2 , AuCl3 , Cu(NO3 )2 , HgCl2 , AgNO3 , SnCl2 , AsCl3 and SbCl3 at sub-toxic concentrations. Methods: The influence of the metal salts on primary human mononuclear cells was analyzed by measuring cell viability with WST assay, apoptosis and necrosis induction by annexin V/propidium iodide staining and proliferation by carboxyfluorescein diacetate succinimidyl ester (CFSE) staining and flow cytometry. Effects on T-cell activation were assessed with CD69 and CD25 expression whereas CD83, CD86 and CD14 expression was measured to evaluate the influence on dendritic cell maturation. Alterations of IL-2 and IFN-␥ secretion were detected by ELISA and genotoxic effects were analyzed using the comet assay. Results: Except for silver nitrate all metal salts showed immunomodulatory effects at for PBMC sub-toxic concentrations. Retardation of cell proliferation was caused by Pb(NO3 )2 , AuCl3 and Cu(NO3 )2 without affecting cell viability. Inhibitory effects on IL-2 secretion were measured after incubation with Pb(NO3 )2 , AuCl3 , Cu(NO3 )2 , HgCl2 and AsCl3 at for the cells sub-toxic concentrations. Conclusion: Cu(NO3 )2 was immunomodulatory at physiological concentrations but all other metal salts showed effects only at dosages at least 10 fold above serum reference ranges. Toxic side-effects of low-concentrated metal preparations are unlikely, because the daily oral intake of metals does not exceed the upper limits which are set in the drinking water ordinance or, in case of AsCl3 , the duration of its application is regulated. http://dx.doi.org/10.1016/j.eujim.2016.08.049
1 ENT
Department Karl Landsteiner Privatuniversity, Mitterweg 10, 3552 Krems Austria 2 Austrian Medial Society for Neural Therapy an Regulation Research (ÖNR) Tannenweg 5, 2451 Hof am Leithaberge Austria E-mail address: [email protected] (F. Gregor). Introduction: Neural therapy is a method of diagnosing and treating illness and pain. NT uses local aesthetics (LA) for local therapy, segmental therapy or finding disturbance fields. Methods: Several Case reports show the benefit of NT in the treatment of functional syndromes and of chronic or acute pain syndromes. In any case, diagnosis is the first step, if infection or cancer isn’t found the next step is treatment. The main topic of NT is pain treatment using LA for local therapy or trigger point therapy. A disturbance field like chr.infektions, scars, teeth has to be found if local treatment fails. In some cases surgery can be a solution for therapy of disturbance fields. Result: There are a lot of functional disorders in the head and neck area like Hyoidtendinopathy, Otalgia and others which can be treated with NT. This lecture will show the principles of NT and my approach as an ENT-specialist in hospital and in a private office. Conclusion: Neural therapy is a safe and simple method of treating many medical problems in head an neck, as an integrative therapy. http://dx.doi.org/10.1016/j.eujim.2016.08.050 48 Integrative approach in diagnosis and treatment of autistic children Momir Dunjic 1,∗ , Zlata Jovanovic Ignjatic 2 , Slaviˇsa Staniˇsi´c 1 , Dejan Krstic 3 , Miodrag Stanisic 2 1 School
of Medicine, University of Pristina, Farmaceutical Phaculty, European University Belgrade, University Alma Mater Europea-ECM (Maribor-Salzburg), Bulevar Despota Stefana 6, 11103 Belgrade, Serbia 2 Serbian Association of Integrative Medicine, Knjeginje Zorke 30, 11000 Belgrade, Serbia 3 Faculty of Occupational Safety, Carnojevi´ ˇ ca 10 a, 18000 Niˇs, Serbia E-mail address: [email protected] (M. Dunjic). Introduction: Autism is connected with brain and liver infection mixed with heavy metal deposit in hyppocampal region as with liver and pancreas enzyme disorders and intolerance of food.
Abstracts / European Journal of Integrative Medicine 8S (2016) 1–66
46
47
Effects of metal salts at sub-toxic concentrations
Impact of neural therapy (NT) as an integrative therapy in head and neck
Carmen Steinborn ∗ , Christoph Diegel, Manuel Garcia-Käufer, Carsten Gründemann, Roman Huber
Fischer Gregor 1,∗ , Jünger Heinz 1,2
Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Breisacher Str. 115B, 79111 Freiburg, Germany E-mail address: [email protected] (C. Steinborn). Introduction: Because different metals are used in complementary medicine for the treatment of diseases related to a dysfunction of the immune system, this study aimed at determining the immunomodulatory potential of Pb(NO3 )2 , AuCl3 , Cu(NO3 )2 , HgCl2 , AgNO3 , SnCl2 , AsCl3 and SbCl3 at sub-toxic concentrations. Methods: The influence of the metal salts on primary human mononuclear cells was analyzed by measuring cell viability with WST assay, apoptosis and necrosis induction by annexin V/propidium iodide staining and proliferation by carboxyfluorescein diacetate succinimidyl ester (CFSE) staining and flow cytometry. Effects on T-cell activation were assessed with CD69 and CD25 expression whereas CD83, CD86 and CD14 expression was measured to evaluate the influence on dendritic cell maturation. Alterations of IL-2 and IFN-␥ secretion were detected by ELISA and genotoxic effects were analyzed using the comet assay. Results: Except for silver nitrate all metal salts showed immunomodulatory effects at for PBMC sub-toxic concentrations. Retardation of cell proliferation was caused by Pb(NO3 )2 , AuCl3 and Cu(NO3 )2 without affecting cell viability. Inhibitory effects on IL-2 secretion were measured after incubation with Pb(NO3 )2 , AuCl3 , Cu(NO3 )2 , HgCl2 and AsCl3 at for the cells sub-toxic concentrations. Conclusion: Cu(NO3 )2 was immunomodulatory at physiological concentrations but all other metal salts showed effects only at dosages at least 10 fold above serum reference ranges. Toxic side-effects of low-concentrated metal preparations are unlikely, because the daily oral intake of metals does not exceed the upper limits which are set in the drinking water ordinance or, in case of AsCl3 , the duration of its application is regulated. http://dx.doi.org/10.1016/j.eujim.2016.08.049
1 ENT
Department Karl Landsteiner Privatuniversity, Mitterweg 10, 3552 Krems Austria 2 Austrian Medial Society for Neural Therapy an Regulation Research (ÖNR) Tannenweg 5, 2451 Hof am Leithaberge Austria E-mail address: [email protected] (F. Gregor). Introduction: Neural therapy is a method of diagnosing and treating illness and pain. NT uses local aesthetics (LA) for local therapy, segmental therapy or finding disturbance fields. Methods: Several Case reports show the benefit of NT in the treatment of functional syndromes and of chronic or acute pain syndromes. In any case, diagnosis is the first step, if infection or cancer isn’t found the next step is treatment. The main topic of NT is pain treatment using LA for local therapy or trigger point therapy. A disturbance field like chr.infektions, scars, teeth has to be found if local treatment fails. In some cases surgery can be a solution for therapy of disturbance fields. Result: There are a lot of functional disorders in the head and neck area like Hyoidtendinopathy, Otalgia and others which can be treated with NT. This lecture will show the principles of NT and my approach as an ENT-specialist in hospital and in a private office. Conclusion: Neural therapy is a safe and simple method of treating many medical problems in head an neck, as an integrative therapy. http://dx.doi.org/10.1016/j.eujim.2016.08.050 48 Integrative approach in diagnosis and treatment of autistic children Momir Dunjic 1,∗ , Zlata Jovanovic Ignjatic 2 , Slaviˇsa Staniˇsi´c 1 , Dejan Krstic 3 , Miodrag Stanisic 2 1 School
of Medicine, University of Pristina, Farmaceutical Phaculty, European University Belgrade, University Alma Mater Europea-ECM (Maribor-Salzburg), Bulevar Despota Stefana 6, 11103 Belgrade, Serbia 2 Serbian Association of Integrative Medicine, Knjeginje Zorke 30, 11000 Belgrade, Serbia 3 Faculty of Occupational Safety, Carnojevi´ ˇ ca 10 a, 18000 Niˇs, Serbia E-mail address: [email protected] (M. Dunjic). Introduction: Autism is connected with brain and liver infection mixed with heavy metal deposit in hyppocampal region as with liver and pancreas enzyme disorders and intolerance of food.