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Effects of oxytocin and steroid horomones on the metabolism of arachidonic acid in the fetal membrane
A.16
Placenta
LOCALIZATION OF HUMAN PLACENTAL GLUCOSE TRANSPORTER 1 (GLUT11 AND GLUCOSE TRANSPORTER 3 (GLUT31 DURING EARLY PREGNANCY. Chikae Tadokoro’, Yasuhiro Yoshimoto*, Masato Yamazaki’, Masahiro Sakata3, Hirohisa Kurach?, Akira Miyake3. Department of Obstetrics and Gynecology, Osaka Rousai Hospital I, Otemae Hospital’and Osaka University Medical SchooF. Objective: To elucidate the potential roles of GLUT1 (Gl) and GLUT3 (G3) in human placenta during early pregnancy, we examined the localization of Gl and G3 by immunohistochemical method. Methods: Cryostat sections of placental tissues at 5-10 weeks of gestational stages wereted with anti Gl or G3 antibody and stained with FITC-labeled streptoavidin. Then they were analyzed with a conforcal lazer microscope. Localization of Gl was further investigated by electron microscopic analysis. Results : Specific staining for Gl was localized on the apical brush border and basal plasma membrane of syncytiotrophoblast (S). The cytotrophoblasts (C) seemed to show immunoreactivity for Gl along the cell membranes at the lightmicroscopic level. However, electron-microscopic analysis revealed that specific staining for Gl was hardly observed on the C. On the other hand, specific staining for G3 was localized on the plasma membrane of C. Conclusions : These findings suggest that Gl may play a role in glucose transporti for fetal growth and that G3 may play a role in glucose transport for the growth of placenta itself.
EFFECTS OF OXYTOCIN AND STEROID HOROMONES ON THE METABOLISM OF ARACHIDONIC ACID IN THE FETAL MEMBRANE. Meijin Tochigi, Buichi Tochigi*, Noriko Takahashi, Haruki Yoshinaga, Miki Nagaoka, Yoshimi Hashimoto, Satoshi Hayakawa and Kazuo Satoh, Department of Obstetrics and Gynecolom, Nihon University School of Medicine, Itabashi, Tokyo, *Department of Obstetrics and Gynecology, Kawaguchi Municipal Medical Center, Kawagichi, Saitama, Japan. In the fetal membranes(FM) oxytocin(OX) and steroid hormones(Es,R) are not well understood with respect to their effects on the process of prostaglandin(PG) production nor for the presence of the mRNA for COX I & II in human parturition. Therefore, using FM and amnion(AM), the effects of OX, E2 and P4 on the up-take of arachidonic acid&A) and the presence of mRNA for COX I &II was investigated. A flask containing MEM with BSA was sealed by FM and soaked in MEM with 14C-AA (0.2 y Ci) added to the fetal side of the membrane. 0X(0.4-4pg/ml), E2 and P4tO.4~ g/ml) were added to the medium and incubated for 1 hr. The up-take of AA by PL in AM after separation by TLC was 29.6 pmol/hr in the controls. OX caused an increase by 20%. In intact FM, the up-take of AA was 61 pmobbr. E2 caused a decrease by 23 %, but, no effect could be demonstrated by OX and P4. COX I & II mRNA by RT-PCR was detected in FM, however their expression did not seem infuluenced by the incubation with OX, E2 and P4. OX accelerated the up-take of AA in AM, but E2 suppressed AM and FM. These findings suggest that the effect of OX and E2 on the production of PG may be mainly a preparatory step for the accumlation of AA in PL in the membranes rather than having a direct action on PG production.
(1995j1. Vol16
Placenta
LOCALIZATION OF HUMAN PLACENTAL GLUCOSE TRANSPORTER 1 (GLUT11 AND GLUCOSE TRANSPORTER 3 (GLUT31 DURING EARLY PREGNANCY. Chikae Tadokoro’, Yasuhiro Yoshimoto*, Masato Yamazaki’, Masahiro Sakata3, Hirohisa Kurach?, Akira Miyake3. Department of Obstetrics and Gynecology, Osaka Rousai Hospital I, Otemae Hospital’and Osaka University Medical SchooF. Objective: To elucidate the potential roles of GLUT1 (Gl) and GLUT3 (G3) in human placenta during early pregnancy, we examined the localization of Gl and G3 by immunohistochemical method. Methods: Cryostat sections of placental tissues at 5-10 weeks of gestational stages wereted with anti Gl or G3 antibody and stained with FITC-labeled streptoavidin. Then they were analyzed with a conforcal lazer microscope. Localization of Gl was further investigated by electron microscopic analysis. Results : Specific staining for Gl was localized on the apical brush border and basal plasma membrane of syncytiotrophoblast (S). The cytotrophoblasts (C) seemed to show immunoreactivity for Gl along the cell membranes at the lightmicroscopic level. However, electron-microscopic analysis revealed that specific staining for Gl was hardly observed on the C. On the other hand, specific staining for G3 was localized on the plasma membrane of C. Conclusions : These findings suggest that Gl may play a role in glucose transporti for fetal growth and that G3 may play a role in glucose transport for the growth of placenta itself.
EFFECTS OF OXYTOCIN AND STEROID HOROMONES ON THE METABOLISM OF ARACHIDONIC ACID IN THE FETAL MEMBRANE. Meijin Tochigi, Buichi Tochigi*, Noriko Takahashi, Haruki Yoshinaga, Miki Nagaoka, Yoshimi Hashimoto, Satoshi Hayakawa and Kazuo Satoh, Department of Obstetrics and Gynecolom, Nihon University School of Medicine, Itabashi, Tokyo, *Department of Obstetrics and Gynecology, Kawaguchi Municipal Medical Center, Kawagichi, Saitama, Japan. In the fetal membranes(FM) oxytocin(OX) and steroid hormones(Es,R) are not well understood with respect to their effects on the process of prostaglandin(PG) production nor for the presence of the mRNA for COX I & II in human parturition. Therefore, using FM and amnion(AM), the effects of OX, E2 and P4 on the up-take of arachidonic acid&A) and the presence of mRNA for COX I &II was investigated. A flask containing MEM with BSA was sealed by FM and soaked in MEM with 14C-AA (0.2 y Ci) added to the fetal side of the membrane. 0X(0.4-4pg/ml), E2 and P4tO.4~ g/ml) were added to the medium and incubated for 1 hr. The up-take of AA by PL in AM after separation by TLC was 29.6 pmol/hr in the controls. OX caused an increase by 20%. In intact FM, the up-take of AA was 61 pmobbr. E2 caused a decrease by 23 %, but, no effect could be demonstrated by OX and P4. COX I & II mRNA by RT-PCR was detected in FM, however their expression did not seem infuluenced by the incubation with OX, E2 and P4. OX accelerated the up-take of AA in AM, but E2 suppressed AM and FM. These findings suggest that the effect of OX and E2 on the production of PG may be mainly a preparatory step for the accumlation of AA in PL in the membranes rather than having a direct action on PG production.
(1995j1. Vol16